1. Draw-fill batch culture mode for production of xylanases by Cellulomonas flavigena on sugar cane bagasse
- Author
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J. Vega-Estrada, Montes-Horcasitas C, Ignacio Magaña-Plaza, Santiago A, and Luis B. Flores-Cotera
- Subjects
Sugar cane ,Applied Microbiology and Biotechnology ,Substrate Specificity ,Industrial Microbiology ,Actinomycetales ,Food science ,Biomass ,Cane ,Sugar ,Cellulose ,biology ,business.industry ,Chemistry ,Cellulomonadaceae ,General Medicine ,biology.organism_classification ,Biotechnology ,Xylan Endo-1,3-beta-Xylosidase ,Xylosidases ,Fermentation ,Xylanase ,Subculture (biology) ,business ,Bagasse - Abstract
Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64 IU mg(-1) protein) and volumetric (166 IU ml(-1)) xylanase activities were obtained by harvesting 50-55% of broth. Values were 3.4 and 3.8 times greater than those obtained in batch cultures carried out under the same conditions. Enzyme productivity of 4.2 IU ml(-1) h(-1) was significantly greater than that obtained in continuous cultures (2.4 IU ml(-1) h(-1)) (P0.05).
- Published
- 2001