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There is intense interest in understanding the degree of damage caused by a pathogen. However, despite abundant evidence that effects of stressors can carry across generations, studies of pathogen virulence have focused almost exclusively on a single generation. Here, we describe transgenerational virulence, where parental exposure to a pathogen reduces offspring fitness.Daphniahost exposure to a microsporidian pathogen significantly reduced lifespan, and in some cases fecundity, of unexposed offspring. These effects carried to the granddaughters, which were smaller if the grandmother had pathogen exposure. A parameterized mathematical model translated these impacts to the population level, finding that transgenerational virulence often reduces host density. Given widespread prior evidence for transgenerational effects of predators, resources, and other factors, we propose that transgenerational virulence may be common.One-Sentence SummaryVirulent effects of pathogens can carry across generations, exacerbating population-level impacts of pathogen outbreaks.

The capacity of arthropod populations to adapt to long-term climatic warming is uncertain. Here, we combine theory and extensive data on diverse arthropod taxa to show that their rate of thermal adaptation to climatic warming will be constrained in two fundamental ways. First, the rate of thermal adaptation is predicted to be limited by the rate of shift in the temperature of peak performance of four life-history traits in a specific order: juvenile development, adult fecundity, juvenile mortality, and adult mortality. Second, thermal adaptation will be constrained due to differences in the temperature of peak performance among these four traits, which are expected to persist because of trade-offs. By compiling a new global dataset of 61 diverse arthropod species, we find strong evidence that contemporary populations have indeed evolved under these constraints. Our results provide a basis for using relatively feasible trait measurements to predict the adaptive capacity of diverse arthropod populations to climatic warming.

Functional responses describe how the proportion of prey consumed by a predator changes as prey density changes. For predators consuming a single prey species, functional responses are determined by two parameters: attack rate and handling time. These parameters may be influenced by morphological and behavioral differences in prey stemming from interspecific or environmentally-driven processes. Here we investigate how interspecific morphological differences and changes in movement rate impact a predator’s functional response. Using a flatworm predator (Stenostomum virginianum) consuming either Paramecium aurelia or P. multimicronucleatum we show that movement rate changes significantly with temperature, leading to changes in attack rate. We also show how body size affects the amount of time predators require to handle prey. We fit a mechanistic functional response model to demonstrate how changes in attack rate and handling time affect overall rates of predation. Our results demonstrate that S. virginianum attack rates are greater for P. aurelia than P. multimicronucleatum. In addition, higher temperature increases S. virginianum attack rates on both species, and reduces the time needed to handle P. aurelia. These differences in predation rate appear related to prey species’ traits, and the temperature-mediated changes in these traits, highlighting the complex processes that underpin predator–prey interactions.

Radiological emergency response may require responders to operate in contaminated environments. To provide more realistic training to these individuals, it has been proposed to disperse low amounts of short-lived radioactive material in simulated emergency scenarios. To demonstrate the applicability and safety of such activities, a limited exercise was conducted where 18F was sprayed in a small area and survey activities were executed. A pre-job external radiation exposure dose assessment was performed in preparation for this training. The research presented here compares participant external recorded doses to assessment results in order to validate the dose estimates. Two individuals were used during the dispersion, search, and survey activities. First, a radiation worker mixed 200 MBq Fludeoxyglucose 18F with 470 mL H2O in a weed sprayer and distributed it over a 3 m × 3 m area. After evaporation, an exercise participant performed search and survey activities in the area. Actual whole-body doses measured with optically stimulated luminescence dosimeters were 10 ± 1 μSv for both personnel. Whole-body digital dosimeters read 4.3 ± 0.2 μSv and 3.3 ± 0.5 μSv for the radiation worker and exercise participant, respectively. Actual extremity doses were below the dosimeters' minimum detectable limits for the radiation worker (thermoluminescence dosimeter) and exercise participant (optically stimulated luminescence dosimeter). The dose assessment-predicted whole-body doses were 2.8 ± 0.4 μSv and 3.2 ± 0.1 μSv for the radiation worker and exercise participant, respectively. The estimated dose to the radiation worker's hand was 21.8 ± 3.8 μSv, and the estimated dose to the exercise participant's knee was 13.4 ± 0.6 μSv. The study provided substantial evidence for the validity of the dose assessment method, supporting its use for a larger training exercise.

Research and theory in nonverbal communication have made great advances toward understanding the patterns and functions of nonverbal behavior in social settings. Progress has been hindered, we argue, by presumptions about nonverbal behavior that follow from both received wisdom and faulty evidence. In this article, we document four persistent misconceptions about nonverbal communication—namely, that people communicate using decodable body language; that they have a stable personal space by which they regulate contact with others; that they express emotion using universal, evolved, iconic, categorical facial expressions; and that they can deceive and detect deception, using dependable telltale clues. We show how these misconceptions permeate research as well as the practices of popular behavior experts, with consequences that extend from intimate relationships to the boardroom and courtroom and even to the arena of international security. Notwithstanding these misconceptions, existing frameworks of nonverbal communication are being challenged by more comprehensive systems approaches and by virtual technologies that ambiguate the roles and identities of interactants and the contexts of interaction.

The overall aim of the project is to identify methods to reduce dog attacks and dog control issues as well as provide evidence-based recommendations to promote responsible dog ownership amongst owners with dog control issues. The project examined contemporary \ud enforcement practice and also explored risk factors related to dog attacks. \ud \ud The project considered both primary and secondary data using a qualitative methodology to: \ud identify what has been published on dangerous dog problems and the factors contributing to the risk of dog bites, strikes and attacks. Empirical research was conducted (primarily qualitative interviews and analysis of enforcement data) to establish (i) if the police and local \ud authorities were using new anti-social behaviour powers and (ii) how effective they were in addressing dog control issues.

Recent improvements to GPU hardware and the symplectic N-body code GENGA allow for unprecedented resolution in simulations of planet formation. In this paper, we report results from high-resolution N-body simulations of terrestrial planet formation that are mostly direct continuation of our previous 10 Myr simulations ( Woo et al., 2021a ) until 150 Myr. By assuming that Jupiter and Saturn have always maintained their current eccentric orbits (EJS), we are able to achieve a reasonably good match to the current inner solar system architecture. However, due to the strong radial mixing that occurs in the EJS scenario, it has difficulties in explaining the known isotopic differences between bodies in the inner solar system, most notably between Earth and Mars. On the other hand, assuming initially circular orbits for Jupiter and Saturn (CJS) can reproduce the observed low degree of radial mixing in the inner solar system, while failing to reproduce the current architecture of the inner solar system. These outcomes suggest a possible paradox between dynamical structure and cosmochemical data for the terrestrial planets within the classical formation scenario. In addition, we use our high-resolution simulations to study the assembly of Earth in unprecedented detail, focusing on its collisional history. We find that more than 90% of giant impacts (GIs) on Earth occur within 80 Myr in the EJS simulations, matching the possible early timing of the Moon-forming GI based on the ages recorded by various meteoritic samples. However, the CJS and EJS scenarios both result in a leftover planetesimal mass that is 1–2 orders of magnitude greater than what currently exists in the solar system. Our overall results strongly suggest the need to consider alternative initial conditions for terrestrial planet formation in the solar system. For example, it may be necessary to assume a lower initial mass in the asteroid belt in order to lower the subsequent effect of radial mixing in the EJS scenario, as well as reduce the mass of leftover planetesimals. Similarly, in the CJS scenario, including an early giant planet instability within 80 Myr could make the scenario dynamically feasible.

We present an instrument that combines high-resolution optical tweezers and multicolor confocal fluorescence spectroscopy along with automated single-molecule assembly. The multicolor allows the simultaneous observation of multiple molecules or multiple degrees of freedom, which allows, for example, the observation of multiple proteins simultaneously within a complex. The instrument incorporates three fluorescence excitation lasers, with a reliable alignment scheme, which will allow three independent fluorescent probe or FRET measurements and also increases flexibility in the choice of fluorescent molecules. We demonstrate the ability to simultaneously measure angstrom-scale changes in tether extension and fluorescence signals. Simultaneous tweezers and fluorescence measurement are particularly challenging because of fluorophore photobleaching, even more so if multiple fluorophores are to be measured. Therefore, (1) fluorescence excitation and detection is interlaced with time-shared dual optical traps. (2) We investigated the photostability of common fluorophores. The mean number of photons emitted before bleaching was unaffected by the trap laser and decreased only slightly with increasing excitation laser intensity. Surprisingly, we found that Cy5 outperforms other commonly used fluorophores by more than fivefold. (3) We devised computer-controlled automation, which conserves fluorophore lifetime by quickly detecting fluorophore-labeled molecule binding, turning off lasers, and moving to add the next fluorophore-labeled component. The single-molecule assembly line enables the precise assembly of multimolecule complexes while preserving fluorophores.

Functional model inks were formulated and printed using flexography in order to assess the influence of ink extensional elasticity and print velocity on the morphological and electrical properties of printed layers. Increased extensional elasticity and higher print velocity resulted in the printing of more isotropic prints, both morphologically and electronically. Furthermore, a correlation between the prints’ morphological and electrical anisotropy strongly suggests that print uniformity has a considerable influence on functionality and that ink rheology may be used to control such characteristics.

Fluid-driven grain flow through a confined channel filled with non-buoyant grains is herein observed to exist in three regimes according to total imposed flow rate. (1) At low imposed flow rates, no grain flow occurs as the fluid stress is insufficient to mobilise the grains and Darcy flow is observed. (2) At a sufficient imposed flow rate, grains begin to flow at the top of the channel with self-similar Gaussian velocity profiles that become faster and encroach deeper into the channel with increased flow rate. (3) At high flow rates, significant grain flow occurs at the base of the channel, distorting the Gaussian profile, resulting in a gradual transition towards a more symmetric, full-channel flow. Each regime, and the transitions between them, is discussed in relation to experimental grain velocity measurements.

Silo discharge has been extensively studied for decades although questions remain regarding the nature of the velocity field, particularly for submerged systems. In this work, fluid-driven granular drainage was performed in a quasi-two-dimensional silo with grains submerged in fluid. While the observed Gaussian velocity profiles were generally consistent with current diffusion models, the diffusion length was found to significantly decrease with height in contrast to the increases previously seen in dry silos. We propose a phenomenological anomalous diffusion model for the spreading of the flow upwards in the cell, with the fluid-driven flows we study here falling in the category of subdiffusive behavior. As the viscous characteristics of the system were amplified, the diffusion length increased and the shape of the flowing zone in the silo changed, deviating further from the parabolic form predicted by traditional normal diffusion models, in effect becoming more subdiffusive as quantified by a decreasing diffusion exponent.

On 11 March 2020, the World Health Organization officially declared COVID-19 a pandemic. The new physical distancing rules have had many consequences, some of which are felt throughout the justice system. Courts across the world limited their operations. Nonetheless, given that justice delayed is justice denied, many jurisdictions have turned to technologies for urgent matters. This paper offers an evidence-based comment and caution for lawyers and judges who could be inclined, for concerns such as cost and time saving, to permanently step aside from in-person trials. Using nonverbal communication research, in conjunction with American and Canadian legal principles, we argue that such a decision could harm the integrity of the justice system.

Recent theoretical and experimental work suggests a frictionless-frictional transition with increasing inter-particle pressure explains the extreme solid-like response of discontinuous shear thickening suspensions. However, analysis of macroscopic discontinuous shear thickening flow in geometries other than the standard rheometry tools remain scarce. Here we use a Hele-Shaw cell geometry to visualise gas-driven invasion patterns in discontinuous shear thickening cornstarch suspensions. We plot quantitative results from pattern analysis in a volume fraction-pressure phase diagram and explain them in context of rheological measurements. We observe three distinct pattern morphologies: viscous fingering, dendritic fracturing, and system-wide fracturing, which correspond to the same packing fraction ranges as weak shear thickening, discontinuous shear thickening, and shear-jammed regimes. The microscopic mechanisms underlying the discontinuous shear thickening transition in dense granular systems are still under debate. Here, the authors explore this transition by characterizing the shape of invasion patterns in Hele-Shaw cell experiments with confined cornstarch suspensions.

This article discusses a new systems model of dyadic nonverbal interaction. The model builds on earlier theories by integrating partners’ parallel sending and receiving nonverbal processes into a broader, dynamic ecological system. It does so in two ways. First, it moves the level of description beyond the individual level to the coordination of both partners’ contributions to the interaction. Second, it recognizes that the relationships between (a) individuals’ characteristics and processes and (b) the social ecology of the interaction setting are reciprocal and best analyzed at the systems level. Thus, the systems model attempts to describe and explain the dynamic interplay among individual, dyadic, and environmental processes in nonverbal interactions. The potential utility and the limitations of the systems model are discussed and the implications for future research considered. Although the systems model is focused explicitly on face-to-face nonverbal communication, it has considerable relevance for digital communication. Specifically, this model provides a useful framework for examining the social effects of mobile device use and as a template for studying human–robot interactions.

In the late stages of terrestrial planet formation, pairwise collisions between planetary-sized bodies act as the fundamental agent of planet growth. These collisions can lead to either growth or disruption of the bodies involved and are largely responsible for shaping the final characteristics of the planets. Despite their critical role in planet formation, an accurate treatment of collisions has yet to be realized. While semi-analytic methods have been proposed, they remain limited to a narrow set of post-impact properties and have only achieved relatively low accuracies. However, the rise of machine learning and access to increased computing power have enabled novel data-driven approaches. In this work, we show that data-driven emulation techniques are capable of classifying and predicting the outcome of collisions with high accuracy and are generalizable to any quantifiable post-impact quantity. In particular, we focus on the dataset requirements, training pipeline, and classification and regression performance for four distinct data-driven techniques from machine learning (ensemble methods and neural networks) and uncertainty quantification (Gaussian processes and polynomial chaos expansion). We compare these methods to existing analytic and semi-analytic methods. Such data-driven emulators are poised to replace the methods currently used in N-body simulations, while avoiding the cost of direct simulation. This work is based on a new set of 14,856 SPH simulations of pairwise collisions between rotating, differentiated bodies at all possible mutual orientations., Computational Astrophysics and Cosmology, 7 (1), ISSN:2197-7909

For security and justice professionals, the thousands of peer-reviewed articles on nonverbal communication represent important sources of knowledge. However, despite the scope of the scientific work carried out on this subject, professionals can turn to programs, methods and approaches that fail to reflect the state of science. The objective of this article is to examine (i) concepts of nonverbal communication conveyed by these programs, methods and approaches, but also (ii) the consequences of their use. To achieve this objective, we describe the scope of scientific research on nonverbal communication. A program (SPOT; “Screening of Passengers by Observation Techniques”), a method (the BAI; “Behavior Analysis Interview”) and an approach (synergology) that each run counter to the state of science are examined. Finally, we outline five hypotheses to explain why some organizations in the fields of security and justice are turning to pseudoscience and pseudoscientific techniques.

Over the past two decades, one of the standard models of protein folding has been the "two-state" model, in which a protein only resides in the folded or fully unfolded states with a single pathway between them. Recent advances in spatial and temporal resolution of biophysical measurements have revealed "beyond-two-state" complexity in protein folding, even for small, single-domain proteins. In this work, we used high-resolution optical tweezers to investigate the folding/unfolding kinetics of the B1 domain of immunoglobulin-binding protein G (GB1), a well-studied model system. Experiments were performed for GB1 both in and out of equilibrium using force spectroscopy. When the force was gradually ramped, simple single-peak folding force distributions were observed, while multiple rupture peaks were seen in the unfolding force distributions, consistent with multiple force-dependent parallel unfolding pathways. Force-dependent folding and unfolding rate constants were directly determined by both force-jump and fixed-trap measurements. Monte Carlo modeling using these rate constants was in good agreement with the force ramp data. The unfolding rate constants exhibited two different behaviors at low vs high force. At high force, the unfolding rate constant increased with increasing force, as previously reported by high force, high pulling speed force ramp measurements. However, at low force, the situation reversed and the unfolding rate constant decreased with increasing force. Taken together, these data indicate that this small protein has multiple distinct pathways to the native state on the free energy landscape.

Confined fluid-driven granular flows are present in a plethora of natural and industrial settings, yet even the most fundamental of these is not completely understood. While widely studied grain flows such as bed load and density-matched Poiseuille flows have been observed to exhibit exponential and Bingham style velocity profiles, respectively, this work finds that a fluid-driven bed of non-buoyant grains filling a narrow horizontal channel—confined both from the sides and above—exhibits self-similar Gaussian velocity profiles. As the imposed flow rate is increased and the grain velocity increases, the Gaussian flow profiles penetrate deeper into the packing of the channel. Filling fractions were observed to be also self-similar and qualitatively consistent with granular theory relating to the viscous number I, which at a given position on the self-similar Gaussian curve is found to be generally constant regardless of the imposed flow rate or velocity magnitude. An empirical description of the flow is proposed, and local velocity and filling fraction measurements were used to obtain the local grain flux and accurately recover a total grain flow rate.

[EN]For security and justice professionals (e.g., police officers, lawyers, judges), the thousands of peer-reviewed articles on nonverbal communication represent important sources of knowledge. However, despite the scope of the scientific work carried out on this subject, professionals can turn to programs, methods, and approaches that fail to reflect the state of science. The objective of this article is to examine (i) concepts of nonverbal communication conveyed by these programs, methods, and approaches, but also (ii) the consequences of their use (e.g., on the life or liberty of individuals). To achieve this objective, we describe the scope of scientific research on nonverbal communication. A program (SPOT; Screening of Passengers by Observation Techniques), a method (the BAI; Behavior Analysis Interview) and an approach (synergology) that each run counter to the state of science are examined. Finally, we outline five hypotheses to explain why some organizations in the fields of security and justice are turning to pseudoscience and pseudoscientific techniques. We conclude the article by inviting these organizations to work with the international community of scholars who have scientific expertise in nonverbal communication and lie (and truth) detection to implement evidence-based practices., [ES]Para los profesionales de la seguridad y la justicia (policías, abogados, jueces), los miles de artículos revisados por pares sobre comunicación no verbal representan fuentes importantes de conocimiento. Sin embargo, a pesar del alcance del trabajo científico realizado sobre este tema, los profesionales pueden recurrir a programas, métodos y enfoques que no reflejan el estado real de la ciencia. El objetivo de este artículo es examinar (i) los conceptos de comunicación no verbal transmitidos por estos programas, métodos y enfoques, pero también (ii) las consecuencias de su uso (por ejemplo, sobre la vida o la libertad de las personas). Para lograr estos objetivos, describimos el alcance de la investigación científica sobre la comunicación no verbal. Se examina un programa (SPOT: Evaluación de pasajeros mediante técnicas de observación), un método (BAI: Entrevista de análisis de conducta) y un enfoque (sinergología) que contradicen el estado de la ciencia. Finalmente, presentamos cinco hipótesis para explicar por qué algunas organizaciones en los campos de la seguridad y la justicia están recurriendo a la pseudociencia y a las técnicas pseudocientíficas. Concluimos el artículo invitando a estas organizaciones a trabajar con la comunidad académica internacional especializada en la investigación sobre comunicación no verbal y detección de mentiras (y verdad) para implementar prácticas basadas en la evidencia.

Toxigenic Vibrio cholerae O1 Inaba, resembling the epidemic Latin American strains (C6706, C6707), was recovered from oysters taken from Mobile Bay, Alabama, on five separate occasions between July 1991 and September 1992. Levels of toxigenic V. cholerae in the oysters, estimated by the most probable number procedure, ranged from 101 to 107 per 100 g. Isolates characterized by pulsed field gel electrophoresis resembled isolates previously recovered from five cargo ships docked at Gulf of Mexico ports. This study details the first reported isolation of toxigenic V. cholerae O1 from oysters in U.S. coastal waters. As with the Gulf Coast strain, the occurrence of the epidemic strain seems to be sporadic and essentially limited to the warmer months.

Oysters and seawater collected from the southeastern United States were examined for fecal coliforms and Escherichia coli , using the current procedure of the American Public Health Association (APHA) and the fluorogenic 4-methylumbelliferyl-β-D-glucuronide (MUG) modified APHA procedure. After the presence of E. coli in both methods was confirmed by conventional IMViC procedures, there was no significant difference between method means at the α = 0.05 level. In oysters, low confirmation rates of 67 and 77% were observed by the APHA and the MUG methods, respectively. Seawater had the greatest confirmation rates (95%) by the MUG method. The MUG method may be a suitable alternative to the current APHA method for the microbiological evaluation of oysters and seawater.

A total of 227 samples, including oysters, shrimp, and water, was collected along the U.S. Gulf Coast and examined to determine the presence of Listeria spp. Listeria spp. were recovered more frequently from shrimp than from water but were not recovered from oysters. Recovery of Listeria spp. from shrimp and waters was improved at temperatures ≤20°C; however, recovery was not affected by salinity or related to the fecal coliform standard for shellfish-growing waters. Although only 5% of the test samples were positive for L. monocytogenes , all Listeria positive shrimp contained L. monocytogenes . The incidence of Listeria spp. in shrimp was low; nevertheless, shrimp represent a potential source of Listeria contamination to processing plants and their products.

Vibrio cholerae non-O1 was found throughout the Apalachicola, FL, estuary. V. cholerae O1 was isolated primarily at the City of Apalachicola sewage treatment plant, Scipio Creek and the north shore of St. George Island. Highest concentrations of both serogroups occurred in August and November. Concentrations were lowest in February and increased substantially in May. A cholera toxin-like toxin was not detected in any of the V. cholerae cultures by the Y-1 mouse adrenal cell assay or the enzyme-linked immunosorbent assay (ELISA). However, 35% of the V. cholerae O1 and 22% of the V. cholerae non-O1 cultures selected for testing caused diarrhea in infant rabbits. The proportion of V. cholerae O1 and non-O1 isolates pathogenic to infant rabbits increased as water temperature decreased. Fecal coliforms appeared to be more useful than total coliforms as indicators of the numbers of V. cholerae in water, but neither of those National Shellfish Sanitation Program indicator groups ensured against the presence of pathogenic V. cholerae in shellfish-growing areas.

Three international methods were evaluated for enumerating Escherichia coli in estuarine waters, oysters ( Crassostrea virginica ), mussels ( Mytilus edilus ) and clams ( Mercenaria mercenaria ). Results of the French most probable number (MPN) method, a modification of the MacKenzie, Taylor and Gilbert (1948) method, were obtained within 48 h and compared favorably with those obtained by the standard American Public Health Association (APHA) MPN procedure in all sample types. Results of the Australian Anderson and Baird-Parker plate count method, obtained within 24 h, were significantly lower than those obtained with the standard APHA procedure for all sample types. Results of the British roll tube method, a 24-h direct count method, compared favorably with the standard APHA procedure only for mussels and waters.

Sigma-1 receptor (S1R) is a unique pluripotent modulator of living systems and has been reported to be associated with a number of neurological diseases including pathological pain. Intrathecal administration of S1R antagonists attenuates the pain behavior of rodents in both inflammatory and neuropathic pain models. However, the S1R localization in the spinal cord shows a selective ventral horn motor neuron distribution, suggesting the high likelihood of S1R in the dorsal root ganglion (DRG) mediating the pain relief by intrathecally administered drugs. Since primary afferents are the major component in the pain pathway, we examined the mouse and rat DRGs for the presence of the S1R. At both mRNA and protein levels, quantitative RT-PCR (qRT-PCR) and Western confirmed that the DRG contains greater S1R expression in comparison to spinal cord, cortex, or lung but less than liver. Using a custom-made highly specific antibody, we demonstrated the presence of a strong S1R immuno-fluorescence in all rat and mouse DRG neurons co-localizing with the Neuron-Specific Enolase (NSE) marker, but not in neural processes or GFAP-positive glial satellite cells. In addition, S1R was absent in afferent terminals in the skin and in the dorsal horn of the spinal cord. Using immuno-electron microscopy, we showed that S1R is detected in the nuclear envelope and endoplasmic reticulum (ER) of DRG cells. In contrast to other cells, S1R is also located directly at the plasma membrane of the DRG neurons. The presence of S1R in the nuclear envelope of all DRG neurons suggests an exciting potential role of S1R as a regulator of neuronal nuclear activities and/or gene expression, which may provide insight toward new molecular targets for modulating nociception at the level of primary afferent neurons.

Inequalities between men and women are common and well-documented. Objective indexes show that men are better positioned than women in societal hierarchies—there is no single country in the world without a gender gap. In contrast, researchers have found that the women-are-wonderful effect—that women are evaluated more positively than men overall—is also common. Cross-cultural studies on gender equality reveal that the more gender egalitarian the society is, the less prevalent explicit gender stereotypes are. Yet, because self-reported gender stereotypes may differ from implicit attitudes towards each gender, we reanalysed data collected across 44 cultures, and (a) confirmed that societal gender egalitarianism reduces the women-are-wonderful effect when it is measured more implicitly (i.e. rating the personality of men and women presented in images) and (b) documented that the social perception of men benefits more from gender egalitarianism than that of women. info:eu-repo/semantics/acceptedVersion

Acetylcholine (ACh)-synthesizing neurons are major components of the enteric nervous system (ENS). They release ACh and peptidergic neurotransmitters onto enteric neurons and muscle. However, pharmacological interrogation has proven inadequate to demonstrate an essential role for ACh. Our objective was to determine whether elimination of ACh synthesis during embryogenesis alters prenatal viability, intestinal function, the neurotransmitter complement, and the microbiome. Conditional deletion of choline acetyltransferase ( ChAT), the ACh synthetic enzyme, in neural crest-derived neurons ( ChAT-Null) was performed. Survival, ChAT activity, gut motility, and the microbiome were studied. ChAT was conditionally deleted in ENS neural crest-derived cells. Despite ChAT absence, mice were born live and survived the first 2 wk. They failed to gain significant weight in the third postnatal week, dying between postnatal d 18 and 30. Small intestinal transit of carmine red was 50% slower in ChAT-Nulls vs. WT and ChAT- Het. The colons of many neonatal ChAT-Null mice contained compacted feces, suggesting dysmotility. Microbiome analysis revealed dysbiosis in ChAT-Null mice. Developmental deletion of ChAT activity in enteric neurons results in proximal gastrointestinal tract dysmotility, critically diminished colonic transit, failure to thrive, intestinal dysbiosis, and death. ACh is necessary for sustained gut motility and survival of neonatal mice after weaning.-Johnson, C. D., Barlow-Anacker, A. J., Pierre, J. F., Touw, K., Erickson, C. S., Furness, J. B., Epstein, M. L., Gosain, A. Deletion of choline acetyltransferase in enteric neurons results in postnatal intestinal dysmotility and dysbiosis.

Acousto-optic (AO) devices have been used extensively in optical tweezers because of their flexibility and speed; however, these devices have trap positioning inaccuracies that limit their usefulness, especially for high-resolution applications. We show that these inaccuracies are due to interference patterns within the AO device sound fields. We have devised a method that removes these inaccuracies by reducing the coherence of the sound fields by directly controlling and randomizing the phase of the radio frequency voltage input signal. We demonstrate that the trapping inaccuracies are eliminated, for both constant trap position and force-ramp measurements, and that no additional noise is added. We show that this random phase method is applicable to both acousto-optic modulator and deflector type devices and can be easily integrated via software upgrade into existing instruments.

During their formation and early evolution, rocky planets undergo multiple global melting events due to accretionary collisions with other protoplanets. The detection and characterization of their post-collision afterglows (magma oceans) can yield important clues about the origin and evolution of the solar and extrasolar planet population. Here, we quantitatively assess the observational prospects to detect the radiative signature of forming planets covered by such collision-induced magma oceans in nearby young stellar associations with future direct imaging facilities. We have compared performance estimates for near- and mid-infrared instruments to be installed at ESO's Extremely Large Telescope (ELT), and a potential space-based mission called Large Interferometer for Exoplanets (LIFE). We modelled the frequency and timing of energetic collisions using \textit{N}-body models of planet formation for different stellar types, and determine the cooling of the resulting magma oceans with an insulating atmosphere. We find that the probability of detecting at least one magma ocean planet depends on the observing duration and the distribution of atmospheric properties among rocky protoplanets. However, the prospects for detection significantly increase for young and close stellar targets, which show the highest frequencies of giant impacts. For intensive reconnaissance with a K band (2.2 $\mu m$) ELT filter or a 5.6 $\mu m$ LIFE filter, the $\beta$ Pictoris, Columba, TW Hydrae, and Tucana-Horologium associations represent promising candidates for detecting a molten protoplanet. Our results motivate the exploration of magma ocean planets using the ELT and underline the importance of space-based direct imaging facilities to investigate and characterize planet formation and evolution in the solar vicinity., Comment: 12 pages, 7 figures, 6 tables; accepted for publication in Astronomy & Astrophysics; associated blog article at https://medium.com/@irene.bonati/spotting-protoplanet-collision-afterglows-in-the-stellar-neighbourhood-f3b65572ec2

Model elastic inks were formulated, rheologically characterised in shear and extension, and printed via flexography to assess the impact of ink elasticity on print uniformity. Flexography is a roll-to-roll printing process with great potential in the mass production of printed electronics for which understanding layer uniformity and the influence of rheology is of critical importance. A new set of flexo-printable Boger fluids was formulated by blending polyvinyl alcohol and high molecular weight polyacrylamide to provide inks of varying elasticity. During print trials, the phenomenon of viscous fingering was observed in all prints, with those of the Newtonian ink exhibiting a continuous striping in the printing direction. Increasing elasticity significantly influenced this continuity, disrupting it and leading to a quantifiable decrease in the overall relative size of the printed finger features. As such, ink elasticity was seen to have a profound effect on flexographic printing uniformity, showing the rheological tuning of inks may be a route to obtaining specific printed features.

Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease affecting spinal cord motoneurons (MN) with an associative connection to Frontotemporal Lobar Dementia (FTLD). The endoplasmic reticulum (ER) bound Sigma-1 Receptor (S1R) chaperone protein localizes to specialized ER cisternae within 10 nm of the plasma membrane in spinal cord ventral horn cholinergic post synaptic C-terminals. Removal of the S1R gene in the Superoxide Dismutase-1 (SOD-1) mouse model of ALS exacerbated the neurodegenerative condition and resulted in a significantly reduced longevity when compared to the SOD-1/S1R wild type (WT) mouse. The proposed amelioration of the ALS phenotype by the S1R is likely due to a “brake” on excitation of the MN as evidenced by a reduction in action potential generation in the MN of the WT when compared to the S1R KO mouse MN. Although the precise signal transduction pathway(s) regulated by the S1R in the MN has/have not been elucidated at present, it is likely that direct or indirect functional interactions occur between the S1R in the ER cisternae with voltage gated potassium channels and/or with muscarinic M2 receptor signaling in the post synaptic plasma membrane. Possible mechanisms for regulation of MN excitability by S1R are discussed.

The membrane bound 223 amino acid Sigma-1 Receptor (S1R) serves as a molecular chaperone and functional regulator of many signaling proteins. Spinal cord motor neuron activation occurs, in part, via large ventral horn cholinergic synapses called C-boutons/C-terminals. Chronic excitation of motor neurons and alterations in C-terminals has been associated with Amyotrophic Lateral Sclerosis (ALS ). The S1R has an important role in regulating motor neuron function. High levels of the S1R are localized in postsynaptic endoplasmic reticulum (ER) subsurface cisternae within 10-20 nm of the plasma membrane that contain muscarinic type 2 acetylcholine receptors (M2AChR), calcium activated potassium channels (Kv2.1) and slow potassium (SK) channels. An increase in action potentials in the S1R KO mouse motor neurons indicates a critical role for the S1R as a "brake" on motor neuron function possibly via calcium dependent hyperpolarization mechanisms involving the aforementioned potassium channels. The longevity of SOD-1/S1R KO ALS mice is significantly reduced compared to SOD-1/WT ALS controls. The S1R colocalizes in C-terminals with Indole(ethyl)amine-N-methyl transferase (INMT ), the enzyme that produces the S1R agonist , N,N'- dimethyltryptamine (DMT). INMT methylation can additionally neutralize endogenous toxic sulfur and selenium derivatives thus providing functional synergism with DMT to reduce oxidative stress in motor neurons . Small molecule activation of the S1R and INMT thus provides a possible therapeutic strategy to treat ALS .

// Timur A. Mavlyutov 1, * , Huan Yang 1, * , Miles L. Epstein 2 , Arnold E. Ruoho 2 , Jay Yang 3 and Lian-Wang Guo 1, 4, 5, 6 1 Department of Surgery, Wisconsin Institute for Medical Research, University of Wisconsin, Madison, WI 53705, USA 2 Department of Neuroscience, University of Wisconsin, Madison, WI 53706, USA 3 Department of Anesthesiology, Wisconsin Institute for Medical Research, University of Wisconsin, Madison, WI 53705, USA 4 McPherson Eye Research Institute, University of Wisconsin, Madison, WI 53705, USA 5 Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA 6 Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA * These authors contributed equally to this work Correspondence to: Lian-Wang Guo, email: Lianwang.Guo@osumc.edu Keywords: the sigma-1 receptor, APEX2-enhanced electron microscopy, nucleoplasmic reticulum, nuclear envelope, serial sectioning Abbreviations: Sig1R-sigma-1 receptor, ER-endoplasmic reticulum, NR-nucleoplasmic reticulum, NE-nuclear envelope, EM-electron microscopy Received: March 02, 2017 Accepted: May 04, 2017 Published: May 16, 2017 ABSTRACT The sigma-1 receptor (Sig1R) is an endoplasmic reticulum chaperonin that is attracting tremendous interest as a potential anti-neurodegenerative target. While this membrane protein is known to reside in the inner nuclear envelope (NE) and influences transcription, apparent Sig1R presence in the nucleoplasm is often observed, seemingly contradicting its NE localization. We addressed this confounding issue by applying an antibody-free approach of electron microscopy (EM) to define Sig1R nuclear localization. We expressed APEX2 peroxidase fused to Sig1R-GFP in a Sig1R-null NSC34 neuronal cell line generated with CRISPR-Cas9. APEX2-catalyzed gold/silver precipitation markedly improved EM clarity and confirmed an apparent intra-nuclear presence of Sig1R. However, serial sectioning combined with APEX2-enhanced EM revealed that Sig1R actually resided in the nucleoplasmic reticulum (NR), a specialized nuclear compartment formed via NE invagination into the nucleoplasm. NR cross-sections also indicated Sig1R in ring-shaped NR membranes. Thus, this study distinguishes Sig1R in the NR which could otherwise appear localized in the nucleoplasm if detected with low-resolution methods. Our finding is important for uncovering potential Sig1R regulations in the nucleus.

Mobile communication technology plays an increasingly pervasive role in everyday life. This study examined one aspect of this role, specifically, the effects of mobile device use on the micro-interactions of pedestrians as they approached and passed a confederate. Over 400 participants were observed in a 2 (group: mobile device vs. control) × 3 [condition: look-only (L); look and smile (LS); look, smile, and greeting (LSG)] factorial design study measuring participants’ looks, smiles, nods, and greetings toward the confederates. Log-linear analyses of the dependent measures provided qualified support for the predicted decreased responsiveness from mobile device users. Specifically, a group by condition interaction on smiles showed that significantly fewer mobile device users than controls smiled at the confederates in the LSG condition. In addition, a group by sex of participant interaction on greetings indicated that significantly fewer female mobile device users offered greetings than males and females in the other conditions. The processes potentially mediating these effects are discussed and the broader influence of mobile devices on the micro-interactions of pedestrians is considered.

Nonverbal communication is a pervasive influence in every life. Furthermore, in most setting, nonverbal communication has a greater impact on interactions than does verbal communication. In this article, we first describe the basic processes involved in nonverbal communication and then briefly examine the factors affecting patterns of nonverbal communication - biology, culture, gender, and personality. Finally, we discuss the role of nonverbal communication in health care settings. Nonverbal communication is an important part of the social skills needed by physicians, nurses, and staff in caring for patients. Specifically, when health care workers attend carefully to the nonverbal behavior of their patients and manage their own behavior to promote rapport, both patients and health care workers benefit.

This article offers a brief, selective review of some important trends and issues in nonverbal research over the last 50 years. Although the volume and range of research have increased dramatically over that period, an adequate integration of this extensive body of work is lacking. In response to this concern, the article proposes that nonverbal communication might be framed in terms of an adaptive and efficient system of managing our social worlds. Several basic characteristics of this system are outlined and their utility in organizing and facilitating research is discussed.

Parenteral nutrition (PN) is a necessary therapy used to feed patients with gastrointestinal dysfunction. Unfortunately, PN results in intestinal atrophy and changes to host immune function. PN may also induce additional effects on gut motility that we hypothesized would result from changes in the enteric nervous system.Mice received an intravenous (i.v.) catheter and were randomized to chow (n = 5), i.v. PN (n = 6), or i.v. PN + bombesin (BBS, 15 μg/kg, 3×/d) (n = 6) for 5 d. Colons were removed and dissected to measure the length and circumference. Enteric neuronal density and neurotransmitter expression were determined by co-immunostaining whole-mount tissue with Hu and neuronal nitric oxide synthase (nNOS).The number of myenteric neurons expressing Hu and nNOS increased per unit length in the mid-colon during PN treatment compared with chow. This increase was abrogated by the addition of BBS to the PN regimen. However, the percentage of nNOS-expressing neurons was not significantly altered by PN. Morphometric analysis revealed a decrease in the length and circumference of the colon during PN administration that was partially normalized by supplementation of PN with BBS. A significant reduction in total fecal output was observed in PN animals compared with chow and was increased by mice receiving BBS in addition to PN.PN causes a constriction of the bowel wall, reducing not only the length but also the circumference of the colon. These changes cause a condensation of enteric neurons but no difference in neurotransmitter expression. BBS supplementation partially restores the constriction and increases the fecal output during PN treatment compared with PN treatment alone.

The membrane bound 223 amino acid Sigma-1 Receptor (S1R) serves as a molecular chaperone and functional regulator of many signaling proteins. Spinal cord motor neuron activation occurs, in part, via large ventral horn cholinergic synapses called C-boutons/C-terminals. Chronic excitation of motor neurons and alterations in C-terminals has been associated with Amyotrophic Lateral Sclerosis (ALS ). The S1R has an important role in regulating motor neuron function. High levels of the S1R are localized in postsynaptic endoplasmic reticulum (ER) subsurface cisternae within 10–20 nm of the plasma membrane that contain muscarinic type 2 acetylcholine receptors (M2AChR), calcium activated potassium channels (Kv2.1) and slow potassium (SK) channels. An increase in action potentials in the S1R KO mouse motor neurons indicates a critical role for the S1R as a “brake” on motor neuron function possibly via calcium dependent hyperpolarization mechanisms involving the aforementioned potassium channels. The longevity of SOD-1/S1R KO ALS mice is significantly reduced compared to SOD-1/WT ALS controls. The S1R colocalizes in C-terminals with Indole(ethyl)amine-N-methyl transferase (INMT ), the enzyme that produces the S1R agonist , N,N′- dimethyltryptamine (DMT). INMT methylation can additionally neutralize endogenous toxic sulfur and selenium derivatives thus providing functional synergism with DMT to reduce oxidative stress in motor neurons . Small molecule activation of the S1R and INMT thus provides a possible therapeutic strategy to treat ALS .

Background Hirschsprung's disease (HSCR) is a congenital condition in which enteric ganglia, formed from neural crest cells (NCC), are absent from the terminal bowel. Dysmotility and constipation are common features of HSCR that persist following surgical intervention. This persistence suggests that the portion of the colon that remains postoperatively is not able to support normal bowel function. To elucidate the defects that underlie this condition, we utilized a murine model of HSCR. Methods Mice with NCC-specific deletion of Ednrb were used to measure the neuronal density and neurotransmitter expression in ganglia. Key Results At the site located proximal to the aganglionic region of P21 Ednrb null mice, the neuronal density is significantly decreased and the expression of neurotransmitters is altered compared with het animals. The ganglia in this colonic region are smaller and more isolated while the size of neuronal cell bodies is increased. The percentage of neurons expressing neuronal nNOS and VIP is significantly increased in Ednrb nulls. Conversely, the percentage of choline acetyltransferase (ChAT) expressing neurons is decreased, while Substance P is unchanged between the two genotypes. These changes are limited to the colon and are not detected in the ileum. Conclusions & Inferences We demonstrate changes in neuronal density and alterations in the balance of expression of neurotransmitters in the colon proximal to the aganglionic region in Ednrb null mice. The reduced neuronal density and complementary changes in nNOS and ChAT expression may account for the dysmotility seen in HSCR.

Both vagal and sacral neural crest cells contribute to the enteric nervous system in the hindgut. Because it is difficult to visualize sacral crest cells independently of vagal crest, the nature and extent of the sacral crest contribution to the enteric nervous system are not well established in rodents. To overcome this problem we generated mice in which only the fluorescent protein-labeled sacral crest are present in the terminal colon. We found that sacral crest cells were associated with extrinsic nerve fibers. We investigated the source, time of appearance, and characteristics of the extrinsic nerve fibers found in the aganglionic colon. We observed that the pelvic ganglion neurons contributed a number of extrinsic fibers that travel within the hindgut between circular and longitudinal muscles and within the submucosa and serosa. Sacral crest-derived cells along these fibers diminished in number from fetal to post-natal stages. A small number of sacral crest-derived cells were found between the muscle layers and expressed the neuronal marker Hu. We conclude that sacral crest cells enter the hindgut by advancing on extrinsic fibers and, in aganglionic preparations, they form a small number of neurons at sites normally occupied by myenteric ganglia. We also examined the colons of ganglionated preparations and found sacral crest-derived cells associated with both extrinsic nerve fibers and nascent ganglia. Extrinsic nerve fibers serve as a route of entry for both rodent and avian sacral crest into the hindgut.

In recent years, the decline of behavioral research in personality and social psychology has attracted renewed attention. The decreased incidence of behavioral research over the past few decades has been documented in the Journal of Personality and Social Psychology and in Personality and Social Psychology Bulletin .This article examined whether this trend was also characteristic of two interdisciplinary language and communication journals ( Human Communication Research and the Journal of Language and Social Psychology) that publish experimental research on interpersonal processes. In contrast to the two personality—social psychology journals, the language and communication journals showed no decrease in behavioral studies over the past two decades. Possible reasons for the contrasting trends in the two types of journals are discussed, including the pattern of increasing numbers of studies per article over time in the personality— social psychology journals, but not in the language and communication journals. Finally, the implications of these differences for research strategies are considered.

The present study examined how communication format, specifically computer-mediated communication vs. face-to-face communication, affected distributive and procedural fairness judgments. Specifically, it was expected that procedural information would have a stronger influence on fairness judgments in face-to-face communication than in computer-mediated communication. This hypothesis was not, however, supported by a significant Mode of Communication × Procedure interaction. We also hypothesized that face-to-face communication, compared with computer-mediated communication, would increase the impact of the distributive information on fairness judgments. As predicted, this hypothesis was supported by a significant Mode of Communication × Outcome interaction. The processes potentially mediating these differences, including psychological distance and evaluation apprehension, are discussed.

Sixty hours of direct measurements of fluorescence were collected from six bowhead whales (Balaena mysticetus) instrumented with fluorometers in Greenland in April 2005 and 2006. The data were used to (1) characterize the three-dimensional spatial pattern of chlorophyll-a (Chl-a) in the water column, (2) to examine the relationships between whale foraging areas and productive zones, and (3) to examine the correlation between whale-derived in situ values of Chl-a and those from concurrent satellite images using the NASA MODIS (Moderate Resolution Imaging Spectroradiometer) EOS-AQUA satellite (MOD21, SeaWifs analogue OC3M and SST MOD37). Bowhead whales traversed 1600 km2, providing information on diving, Chl-a structure and temperature profiles to depths below 200 m. Feeding dives frequently passed through surface waters (>50 m) and targeted depths close to the bottom, and whales did not always target patches of high concentrations of Chl-a in the upper 50 m. Five satellite images were available wi...

The enteric nervous system (ENS) develops from neural crest cells (NCCs)that enter the foregut and hindgut to become enteric neural-crest-derived cells (ENCCs). When these cells of neural crest origin fail to colonize the terminal hindgut, this aganglionic region becomes non-functional and results in a condition in humans known as Hirschsprung's disease (HSCR). One of the genes associated with HSCR is endothelin receptor type B (Ednrb). To study the development of colonic aganglionosis we have utilized a novel knockout mouse (Ednrbflex3/flex3), in which the expression of a null Ednrb allele and YFP is confined to NCCs. We have identified two primary cellular defects related to defective EDNRB signaling. First, ENCC advance in Ednrbflex3/flex3 embryos is delayed shortly after NCCs enter the gut. Apart from this early delay, Ednrbflex3/flex3 ENCCs advance normally until reaching the proximal colon. Second, as Ednrbflex3/flex3 ENCCs reach the colon at E14.5, they display migratory defects, including altered trajectories and reduced speed, that are not dependent on proliferation or differentiation. We constructed grafts to test the ability of donor ENCCs to invade a recipient piece of aganglionic colon. Our results indicate that the age of the recipient, and not the age or genotype of donor ENCCs, determines whether the colon is invaded. We identify changes in laminin expression that are associated with the failure of ENCCs to invade recipient tissue. Together,our data suggest that a defect in pre-enteric Ednrbflex3/flex3 NCCs results in delayed colonic arrival,which, due to environment changes in the colon, is sufficient to cause aganglionosis.