151 results on '"Miao, Lei"'
Search Results
2. Thiourea-Assisted Facile Fabrication of High-Quality CsPbBr3 Perovskite Films for High-Performance Solar Cells
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Jiayu Bi, Jiarun Chang, Miao Lei, Wei Zhang, Fanning Meng, and Guiqiang Wang
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General Materials Science - Published
- 2022
3. Optimal wireless rate and power control in the presence of jammers using reinforcement learning
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Raji, Fadlullah and Miao, Lei
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Computer Science - Networking and Internet Architecture ,Networking and Internet Architecture (cs.NI) ,FOS: Computer and information sciences ,FOS: Electrical engineering, electronic engineering, information engineering ,Systems and Control (eess.SY) ,Electrical Engineering and Systems Science - Systems and Control - Abstract
Future wireless networks require high throughput and energy efficiency. This paper studies using Reinforcement Learning (RL) to do transmission rate and power control for maximizing a joint reward function consisting of both throughput and energy consumption. We design the system state to include factors that reflect packet queue length, interference from other nodes, quality of the wireless channel, battery status, etc. The reward function is normalized and does not involve unit conversion. It can be used to train three different types of agents: throughput-critical, energy-critical, and throughput and energy balanced. Using the NS-3 network simulation software, we implement and train these agents in an 802.11ac network with the presence of a jammer. We then test the agents with two jamming nodes interfering with the packets received at the receiver. We compare the performance of our RL optimal policies with the popular Minstrel rate adaptation algorithm: our approach can achieve (i) higher throughput when using the throughput-critical reward function; (ii) lower energy consumption when using the energy-critical reward function; and (iii) higher throughput and slightly higher energy when using the throughput and energy balanced reward function. Although our discussion is focused on 802.11ac networks, our method is readily applicable to other types of wireless networks., Comment: Published in International Telecommunication Union, Journal on Future and Evolving Technologies (ITU J-FET) 2022
- Published
- 2022
4. Electrical Signal Initiates Kinetic Assembly of Collagen to Construct Optically Transparent and Geometry Customized Artificial Cornea Substitutes
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Miao Lei, Shaohua Zhang, Hang Zhou, Haoran Wan, Yi Lu, Shaoliang Lin, Jianguo Sun, Xue Qu, and Changsheng Liu
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Cornea ,Wound Healing ,Optics and Photonics ,Corneal Stroma ,General Engineering ,Animals ,Humans ,General Physics and Astronomy ,General Materials Science ,Rabbits ,Collagen ,Prostheses and Implants - Abstract
Corneal transplantation is an effective treatment for reconstructing injured corneas but is very limited due to insufficient donors, which has led to a growing demand for development of artificial corneal substitutes (ACSs). Collagen is a potential building block for ACS fabrication, whereas technically there are limited capabilities to control the collagen assembly for creating highly transparent collagen ACSs. Here, we report an electro-assembly technique to kinetically control collagen assembly on the nanoscale that allows the yielding collagen ACSs with structure determined superior optics. Structurally, the kinetically electro-assembled collagen (KEA-Col) is composed of partially aligned microfibrils (∼10 nm in diameter) with compacted lamellar organization. Optical analysis reveals that such microstructure is directly responsible for its optimal light transmittance by reducing light scattering. Moreover, this method allows the creation of complex three-dimensional geometries and thus is convenient to customize collagen ACSs with specific curvatures to meet refractive power requirements. Available properties (e.g., optics and mechanics) of cross-linked KEA-Cols were studied to meet the clinical requirement as ACSs, and
- Published
- 2022
5. Job Burnout amongst University Administrative Staff Members in China—A Perspective on Sustainable Development Goals (SDGs)
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Hassan, Miao Lei, Gazi Mahabubul Alam, and Aminuddin bin
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burnout ,emotion regulation ,SDGs ,sustainable education ,university administrative staff - Abstract
It is widely accepted that administrative staff, as important components of a university’s workforce, play a critical role in realizing the United Nations’ Sustainable Development Goals (SDGs). The worth of administrative staff is based on their productivity, and this has a significant impact on the viability of universities. Based on the job demands–resources model, this study investigates the antecedents of job burnout among administrative staff from both emotional and interpersonal perspectives, taking into account SDGs 3, 4, and 8. In this paper, a quantitative research method using descriptive and inferential analyses explores the complex interplay between job autonomy, emotional job demands, colleague support, and job burnout, with a particular emphasis on the role of emotion regulation. A questionnaire was answered anonymously by 1009 administrative staff members in China, and the results conclude that job autonomy was negatively associated with job burnout, while emotional job demands were positively linked to burnout. Moreover, leader support emerged as more beneficial to workers than colleague support. Emotion regulation strategies such as reappraisal function as an important personal resource that buffers the negative effects of job demands and enhances job resources, leading to lower levels of burnout. Furthermore, this study examines how the SDGs can be achieved through reducing job burnout. The important implications for university administrative staff and policymakers, as well as the sustainable development of universities, are discussed.
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- 2023
- Full Text
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6. Electro-Biofabrication. Coupling Electrochemical and Biomolecular Methods to Create Functional Bio-Based Hydrogels
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Yi Liu, Eunkyoung Kim, Miao Lei, Si Wu, Kun Yan, Jana Shen, William E. Bentley, Xiaowen Shi, Xue Qu, and Gregory F. Payne
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Biomaterials ,Polymers and Plastics ,Materials Chemistry ,Bioengineering - Published
- 2023
7. Variant rs8400 enhances ALKBH5 expression through disrupting miR-186 binding and promotes neuroblastoma progression
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Guan, Qian, Lin, Huiran, Hua, Wenfeng, Lin, Lei, Liu, Jiabin, Deng, Linqing, Zhang, Jiao, Cheng, Jiwen, Yang, Zhonghua, Li, Yong, Bian, Jun, Zhou, Haixia, Li, Suhong, Li, Li, Miao, Lei, Xia, Huimin, He, Jing, and Zhuo, Zhenjian
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Original Article - Abstract
OBJECTIVE: AlkB homolog 5 (ALKBH5) has been proven to be closely related to tumors. However, the role and molecular mechanism of ALKBH5 in neuroblastomas have rarely been reported. METHODS: The potential functional single-nucleotide polymorphisms (SNPs) in ALKBH5 were identified by National Center for Biotechnology Information (NCBI) dbSNP screening and SNPinfo software. TaqMan probes were used for genotyping. A multiple logistic regression model was used to evaluate the effects of different SNP loci on the risk of neuroblastoma. The expression of ALKBH5 in neuroblastoma was evaluated by Western blotting and immunohistochemistry (IHC). Cell counting kit-8 (CCK-8), plate colony formation and 5-ethynyl-2’-deoxyuridine (EdU) incorporation assays were used to evaluate cell proliferation. Wound healing and Transwell assays were used to compare cell migration and invasion. Thermodynamic modelling was performed to predict the ability of miRNAs to bind to ALKBH5 with the rs8400 G/A polymorphism. RNA sequencing, N6-methyladenosine (m(6)A) sequencing, m(6)A methylated RNA immunoprecipitation (MeRIP) and a luciferase assay were used to identify the targeting effect of ALKBH5 on SPP1. RESULTS: ALKBH5 was highly expressed in neuroblastoma. Knocking down ALKBH5 inhibited the proliferation, migration and invasion of cancer cells. miR-186-3p negatively regulates the expression of ALKBH5, and this ability is affected by the rs8400 polymorphism. When the G nucleotide was mutated to A, the ability of miR-186-3p to bind to the 3’-UTR of ALKBH5 decreased, resulting in upregulation of ALKBH5. SPP1 is the downstream target gene of the ALKBH5 oncogene. Knocking down SPP1 partially restored the inhibitory effect of ALKBH5 downregulation on neuroblastoma. Downregulation of ALKBH5 can improve the therapeutic efficacy of carboplatin and etoposide in neuroblastoma. CONCLUSIONS: We first found that the rs8400 G>A polymorphism in the m(6)A demethylase-encoding gene ALKBH5 increases neuroblastoma susceptibility and determines the related mechanisms. The aberrant regulation of ALKBH5 by miR-186-3p caused by this genetic variation in ALKBH5 promotes the occurrence and development of neuroblastoma through the ALKBH5-SPP1 axis.
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- 2023
8. Interface Modification via a Hydrophobic Polymer Interlayer for Highly Efficient and Stable Carbon-Based Inorganic Perovskite Solar Cells
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Guiqiang Wang, Jiayu Bi, Miao Lei, Jiarun Chang, and Fanning Meng
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Fuel Technology ,General Chemical Engineering ,Energy Engineering and Power Technology - Published
- 2022
9. Microstructure and mechanical properties of VAlN/Si3N4 nano-multilayer coatings
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Xian-cheng Tao, Yu-min Lou, Miao-lei Li, Ning-ning Zhao, Xiu-zhi Tang, Hai-long Hu, Xiao-zhong Huang, and Jian-ling Yue
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Metals and Alloys ,General Engineering - Published
- 2022
10. An experimental study of redistribution of income:People's opinions and Their guess
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Sobei H. Oda and Miao, Lei
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Economics ,FOS: Law ,Social and Behavioral Sciences ,Law - Abstract
Even in a single society, different people may have different opinions on fairness, of which a few may be considered to be legitimate. In income redistribution, people will choose one of them to claim that a redistribution is fair, considering their own interests. Underprivileged would demand equal distribution, while privileged people would assert the present distribution is so fair that no distribution is necessary. In short, though they may refer only to how the present income distribution is determined to support their opinions on fairness, people take account of what concept of fairness is favorable to them in their mind. Consider purely, eliminating the effect of how to get the initial income such as effort ,talent and luck and something else, we just focus on even subjects get the money occasionally, they still respect the received money, and lead to an effect to their fairness consideration, then show an different redistribution behavior. We have already conduct some experiments from 2018 to 2019. Due to the Covid-19, it's hard to recruit subjects to attend experiments in experimental Lab. But now for the policies and environment of Covid-19 is changed. It's possible for us to conduct the Lab experiments. Therefore, we would like to do the further researches on the same research problem. Forever, check wether peoples' options have changed after the experience of Covid-19.
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- 2023
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11. Rapid and Effective Adsorptive Removal of Antimony(Ⅴ) from Water by Calcined Ni-Al Layered Double Hydroxide
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Yuchen Guo, Ye Li, Yi Ke, Bolin Li, Yan Fu, Dongxue Liu, Chang Liu, and Miao Lei
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- 2023
12. A Suture-Free, Shape Self-Adaptive and Bioactive PEG-Lysozyme Implant for Corneal Stroma Defect Repair and Rapid Vision Restoration
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Hang Zhou, Shaohua Zhang, Miao Lei, Yixin Cai, Honglei Wang, Jianguo Sun, Jingyuan Cui, Changsheng Liu, and Xue Qu
- Published
- 2023
13. Advances and applications in liver organoid technology
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Li LIN, Miao LEI, JiaMan LIN, and WenXiang HU
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Pharmacology (medical) - Published
- 2022
14. Bandgap tuning of a CsPbBr3 perovskite with synergistically improved quality via Sn2+ doping for high-performance carbon-based inorganic perovskite solar cells
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Guiqiang Wang, Jiayu Bi, Jiarun Chang, Miao Lei, Huiling Zheng, and Yu Yan
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Inorganic Chemistry - Abstract
Partially substituting Pb2+ with an appropriate amount of Sn2+ simultaneously reduces the bandgap and improves the quality and morphology of a CsPbBr3 perovskite film.
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- 2022
15. Constructing of a stable Ti3C2 MXenes nano-traps with ultrahigh and selectively uranyl encapsulation capability by polyethyleneimine intercalation
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Chang Liu, Ye Li, Dongxue Liu, Miao Lei, Bolin Li, Chengbin Fu, and Jun Zhang
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General Chemical Engineering ,Environmental Chemistry ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2023
16. Osteofibrous dysplasia-like adamantinoma: A case report and literature review
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Li, Jian-Wei, Miao, Lei, Zhao, Zhen-Guo, Yang, Lin, Shi, Zhuo, and Li, Meng
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Cancer Research ,Oncology - Published
- 2022
- Full Text
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17. Thiourea-Assisted Facile Fabrication of High-Quality CsPbBr
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Jiayu, Bi, Jiarun, Chang, Miao, Lei, Wei, Zhang, Fanning, Meng, and Guiqiang, Wang
- Abstract
Inorganic CsPbBr
- Published
- 2022
18. Research on the Multi-Element Synthetic Aperture Focusing Technique in Breast Ultrasound Imaging, Based on the Ring Array
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Yang Wu, Wendong Zhang, Xingling Shao, Yuhua Yang, Tian Zhang, Miao Lei, Zhihao Wang, Bizhen Gao, and Shumin Hu
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Control and Systems Engineering ,Mechanical Engineering ,ultrasonography ,breast imaging ,ring array ,synthetic aperture ,multi-element synthetic aperture focusing ,Electrical and Electronic Engineering - Abstract
As a widely clinical detection method, ultrasonography (US) has been applied to the diagnosis of breast cancer. In this paper, the multi-element synthetic aperture focusing (M-SAF) is applied to the ring array of breast ultrasonography (US) imaging, which addresses the problem of low imaging quality due to the single active element for each emission and the reception in the synthetic aperture focusing. In order to determine the optimal sub-aperture size, the formula is derived for calculating the internal sound pressure of the ring array with a 200 mm diameter, and the sound pressure distribution is analyzed. The ring array with 1024 elements (1024 ring array) is established in COMSOL Multiphysics 5.6, and the optimal sub-aperture size is 16 elements, according to the sound field beam simulation and the directivity research. Based on the existing experimental conditions, the ring array with 256 elements (256 ring array) is simulated and verified by experiments. The simulation has a spatial resolution evaluation in the k-Wave toolbox, and the experiment uses nylon rope and breast model imaging. The results show that if the sub-aperture size has four elements, the imaging quality is the highest. Specifically, the spatial resolution is the best, and the sound pressure amplitude and signal-to-noise ratio (SNR) are maintained at a high level in the reconstructed image. The optimal sub-aperture theory is verified by the two kinds of ring arrays, which also provide a theoretical basis for the application of the multi-element synthetic aperture focusing technology (M-SAF) in ring arrays.
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- 2022
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19. Inconsistent Frequency Trends Between Hourly and Daily Precipitation During Warm Season in Mainland of China
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Miao Lei, Jiming Li, Lijie Zhang, Cong Deng, Yarong Li, and Jianjun He
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Geophysics ,General Earth and Planetary Sciences - Published
- 2022
20. Hierarchical porous carbon obtained from directly carbonizing carex meyeriana for high-performance supercapacitors
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Wei Zhang, Guiqiang Wang, Jieqiong Liu, Miao Lei, and Jiayu Bi
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Supercapacitor ,Materials science ,Carbonization ,chemistry.chemical_element ,Electrolyte ,Condensed Matter Physics ,Capacitance ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,chemistry ,Chemical engineering ,Electrical and Electronic Engineering ,Mesoporous material ,Carbon ,Current density ,Power density - Abstract
Hierarchical porous carbon materials with high surface area are facilely prepared by directly carbonizing carex meyeriana without any extra activation procedure. The as-prepared porous carbon samples possess high Brunauer–Emmett–Teller (BET) surface areas (in the ~ 518–742 m2 g−1 range) and unique hierarchical porous structure containing macropore channels and mesopores and micropores developed in the wall of macropores. These intriguing characteristics make the as-prepared hierarchical porous carbon samples a promising electrode material for supercapacitors. The capacitive performance was measured in the three-electrode system with 6 M KOH electrolyte. The hierarchical porous carbon prepared at the carbonization temperature of 1000 °C presents a high specific capacitance of 178.6 F g−1 at a current density of 0.5 A g−1, a good rate performance ( about 65.2% retention ratio at the current density of 20 A g−1), and an excellent cycling stability (no obvious performance fading after 10,000 cycles). In addition, the fabricated two-electrode device achieves an energy density of 4.33 Wh kg−1 at a high power density of 5 kW kg−1. These results provide a green and facile method to synthesize the electrode material from biomass for high-performance supercapacitors.
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- 2021
21. In suit establishment of Ti3C2 MXenes exchanger via Na+, K+ and NH4+ intercalation: exceptionally UO22+ loading capability and hijacking mechanism
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Chang Liu, Ye Li, Dongxue Liu, Miao Lei, Bolin Li, Chengbin Fu, and Jun Zhang
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General Chemical Engineering ,Environmental Chemistry ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2023
22. Yes-associated protein inhibition ameliorates liver fibrosis and acute and chronic liver failure by decreasing ferroptosis and necroptosis
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Wen Zhao, Miao Lei, Jinfeng Li, Hailin Zhang, Hongkun Zhang, Yuxin Han, Zhiwei Ba, Manli Zhang, Dongdong Li, and Chuanmiao Liu
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Multidisciplinary - Published
- 2023
23. Acute-on-chronic liver failure in patients with metabolic associated fatty liver disease: Past, Present, and Future
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MIAO Lei
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RC799-869 ,Diseases of the digestive system. Gastroenterology - Abstract
Metabolic associated fatty liver disease (MAFLD) is a hotspot in the field of fatty liver disease at present and it has become the most common chronic liver disease around the world. It is predicted that the incidence rates of MAFLD and related liver cirrhosis will continue to grow in the next 20 years and that they will become new global health issues. Acute-on-chronic liver failure (ACLF) is defined as a clinical syndrome of acute or subacute liver function decompensation within a short period of time in the presence of existing chronic liver diseases, with the main clinical manifestations of ascites, jaundice, coagulation disorder, and hepatic encephalopathy. Based on the existing data, this article discusses the epidemiology, pathogenesis, treatment and management strategies, and future prospects of MAFLD-ACLF.
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- 2021
24. Water Resistance Properties of Lime Soil Ground under Long-Term Soaking Environment
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Xin-hao Yuan, Miao-lei Hou, Tian-shun Hou, Xin Wang, Zhi-zhong Zhang, You-dong Ceng, and Xuan Zeng
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021110 strategic, defence & security studies ,Toughness ,Materials science ,Absorption of water ,Soil test ,0211 other engineering and technologies ,02 engineering and technology ,engineering.material ,Geotechnical Engineering and Engineering Geology ,Brittleness ,Surface-area-to-volume ratio ,Immersion (virtual reality) ,engineering ,Geotechnical engineering ,Ductility ,021101 geological & geomatics engineering ,Lime - Abstract
To explore the water resistance properties of lime soil ground, the physical and mechanical properties of lime soil with 5%, 10%, 15% and 20% lime content after long-term water immersion were studied by conducting unconfined compressive tests, density tests and water absorption tests. The results show that the stress–strain relationship curves of the lime soil during immersion are of the strain-softening type. The curves are multistage and nonlinear with evident peak values, and the lime soil achieves ductility and toughness with an increase in immersion time. The failure modes of the lime soil samples mainly include single slope shear failures, brittle splitting failures and conjugate shear failures, which are mainly affected by the lime content and immersion time. The strength of the lime soil decreases sharply in a short time while interacting with water. The strength slowly increases to 50.07–69.15% of the initial strength after immersion for a long time, i.e., still far less than the initial strength without immersion. Under long-term immersion, the water absorption of the lime soil increases gradually at the initial stage of immersion and then increases to a stable value of 16.76–21.67%. The change law of the wet density is essentially controlled by the water absorption. The strength of lime soil decreases significantly, and the water resistance capacity is poor after soaking; lime soil with 15% lime content has better engineering properties. It is recommended to apply lime soil with 15% lime content in practical engineering with a volume ratio of lime to soil of 3:7 and to avoid using lime soil materials in immersed or other dry-humid environments.
- Published
- 2021
25. Bifunctional Lewis Base‐Assisted Fabrication of High‐Quality CsPbIBr 2 Perovskite for Efficient and Stable Solar Cells
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Jiayu Bi, Jiarun Chang, Miao Lei, Fanning Meng, and Guiqiang Wang
- Subjects
General Energy - Published
- 2023
26. Variation Characteristics of Pavement Temperature in Winter and Its Nowcasting for Xianyang Airport Expressway, China
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Feng, Lei, Tian, Hua, Yuan, Xiaoyu, Miao, Lei, and Lin, Mingyu
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Atmospheric Science ,forecast validation ,nowcasting ,Environmental Science (miscellaneous) ,pavement temperature ,variation characteristics - Abstract
Based on the pavement temperature observation data of the transportation meteorological stations along the Xianyang Airport Expressway, China, as well as the datasets of precipitation and sunshine hours obtained from the nearby weather stations, the variation characteristics of local pavement temperatures are investigated for winter in this study. Results indicate that during the daytime, the pavement temperatures are always higher on sunny and cloudy days than those on rainy and snowy days, while during the nighttime, the temperatures on sunny and cloudy days are higher than those on the days with freezing rain and snow, and with the temperatures on rainy and snowy days without icing being further higher. In general, the pavement temperatures in winter features significant periodic oscillations with cycles of roughly 24 h, 12 h, 8 h, 6 h, 5 h and 4 h, which differ slightly at different times for different stations. Moreover, the nowcasting experiments on the local pavement temperatures are also carried out using a regression model via extracting the corresponding periodic features. It shows the mean absolute errors of about 0.6 °C, 1.2 °C, and 1.5 °C for lead times of 1 h, 2 h, and 3 h, respectively. The nowcasting skills are higher on rainy and snowy days, while are inferior on sunny days. For nowcasting cases initialized at nighttime (daytime), the mean absolute errors are 0.4 °C (0.7 °C) and 0.9 °C (1.4 °C) for lead times of 1 h and 2 h. Examinations suggest that the nowcasting system could be well utilized in plain areas of China, whereas it shows relatively larger biases in plateau areas with complex terrain.
- Published
- 2023
27. Compressed sensing approach for CMUT sparse array in multi-element synthetic transmit aperture
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Tian Zhang, Wendong Zhang, Xingling Shao, Yuhua Yang, Yang Wu, Miao Lei, and Zhihao Wang
- Subjects
Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
28. Old and new classes of glucose-lowering agents as treatments for non-alcoholic fatty liver disease: a narrative review
- Author
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Miao, Lei, Xu, Jing, Targher, Giovanni, Byrne, Christopher, and Zheng, Ming-Hua
- Subjects
endocrine system diseases ,nutritional and metabolic diseases ,digestive system ,digestive system diseases - Abstract
Non-alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease with a global prevalence of about 55% in people with type 2 diabetes mellitus (T2DM). T2DM, obesity and NAFLD are three closely inter-related pathological conditions. In addition, T2DM is one of the strongest clinical risk factors for the faster progression of NAFLD to non-alcoholic steatohepatitis (NASH), cirrhosis and hepatocellular carcinoma. Increasing evidence suggests that newer classes of glucose-lowering drugs, such as peroxisome proliferator-activated receptor agonists, glucagon-like peptide-1 receptor agonists, dipeptidyl peptidase-4 inhibitors or sodium-glucose cotransporter-2 inhibitors, could reduce the rates of NAFLD progression. This narrative review aims to briefly summarize the recent results from randomized controlled trials testing the efficacy and safety of old and new glucose-lowering drugs for the treatment of NAFLD or NASH in adults both with and without coexisting T2DM.
- Published
- 2022
29. Electro-assembly of a dynamically adaptive molten fibril state for collagen
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Miao Lei, Xue Qu, Haoran Wan, Dawei Jin, Shijia Wang, Zhiling Zhao, Meng Yin, Gregory F. Payne, and Changsheng Liu
- Subjects
Multidisciplinary - Abstract
Collagen is a biological building block that is hierarchically assembled into diverse morphological structures that, in some cases, is dynamically adaptive in response to external cues and in other cases forms static terminal structures. Technically, there is limited capabilities to guide the emergence of collagen’s hierarchical organization to recapitulate the richness of biological structure and function. Here, we report an electro-assembly pathway to create a dynamically adaptive intermediate molten fibril state for collagen. Structurally, this intermediate state is composed of partially aligned and reversibly associating fibrils with limited hierarchical structure. These molten fibrils can be reversibly reconfigured to offer dynamic properties such as stimuli-stiffening, stimuli-contracting, self-healing, and self-shaping. Also, molten fibrils can be guided to further assemble to recapitulate the characteristic hierarchical structural features of native collagen (e.g., aligned fibers with D-banding). We envision that the electro-assembly of collagen fibrils will provide previously unidentified opportunities for tailored collagen-based biomedical materials.
- Published
- 2022
30. Additional file 1 of Prediction of the therapeutic efficacy of epirubicin combined with ifosfamide in patients with lung metastases from soft tissue sarcoma based on contrast-enhanced CT radiomics features
- Author
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Miao, Lei, Ma, Shu-Tao, Jiang, Xu, Zhang, Huan-Huan, Wang, Yan-Mei, and Li, Meng
- Abstract
Additional file 1. Supplementary Material.
- Published
- 2022
- Full Text
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31. Hierarchical Porous Carbon Arrays Derived from Directly Carbonizing Carex Meyeriana for Counter Electrodes of High-Efficiency Dye-Sensitized Solar Cells
- Author
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Miao Lei, Wei Zhang, Guiqiang Wang, Jieqiong Liu, and Weinan Dong
- Subjects
Materials science ,General Chemical Engineering ,Energy Engineering and Power Technology ,chemistry.chemical_element ,02 engineering and technology ,Electrolyte ,021001 nanoscience & nanotechnology ,Electrochemistry ,Tin oxide ,law.invention ,Dye-sensitized solar cell ,Fuel Technology ,020401 chemical engineering ,chemistry ,Chemical engineering ,law ,Specific surface area ,Electrode ,Solar cell ,0204 chemical engineering ,0210 nano-technology ,Carbon - Abstract
Hierarchical porous carbon arrays (HPCAs) are facilely produced by directly carbonizing Carex meyeriana through a self-activated and self-template process. The morphology analysis and nitrogen sorption measurements reveal that the obtained HPCAs have a large specific surface area and unique hierarchical pore structure comprising macropore channel arrays and micropores and mesopores developed on the wall of macropore channels. The HPCA electrodes are subsequently prepared by depositing HPCAs to the fluorine-doped tin oxide conductive substrate. Electrochemical tests demonstrate that the HPCA electrode shows a superior electrocatalytic activity for the regeneration of the I–/I3– electrolyte. The superior electrocatalytic activity of HPCA electrodes is mainly due to the combined effect of unique hierarchical pore architecture and large surface area, which facilitates the electrolyte diffusion and provides the abundant effective electrocatalytic active sites. Under 1 sun illumination, the HPCA electrode-based dye-sensitized solar cell displays a gratifying conversion efficiency of 6.45%, which is close to that based on commonly used Pt electrodes.
- Published
- 2020
32. Simple and green fabrication of a biomass-derived N and O self-doped hierarchical porous carbon via a self-activation route for supercapacitor application
- Author
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He Jingjing, Wang Kunjie, Li Hongxia, Ren Ruiye, Yang Binbin, Zhang Deyi, Wang Yuling, Wang Yi, Miao Lei, and Xie Mei
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Fabrication ,Materials science ,Energy Engineering and Power Technology ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Capacitance ,Inorganic Chemistry ,Specific surface area ,Materials Chemistry ,medicine ,Supercapacitor ,Renewable Energy, Sustainability and the Environment ,Process Chemistry and Technology ,Organic Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Chemical engineering ,chemistry ,Ceramics and Composites ,Degradation (geology) ,0210 nano-technology ,Current density ,Carbon ,Activated carbon ,medicine.drug - Abstract
To meet the increased performance and cost requirements of commercial supercapacitor, a N and O self-doped hierarchical porous carbon is fabricated via a green and simple self-activation route utilizing leaves of wild hollyhock as raw materials. Comparing to commercial activated carbon, the reported material exhibits some marked merits, such as simple and green fabrication process, low cost, and superior capacitance performance. The specific surface area of the obtained N and O co-doped hierarchical porous carbon arrives 954 m2 g−1, and the content of the self-doped nitrogen and oxygen reaches 2.64 at.% and 7.38 at.%, respectively. The specific capacitance of the obtained material reaches 226 F g−1 while the specific capacitance of the symmetric supercapacitor arrives 47.3 F g−1. Meanwhile, more than 90.3% of initial specific capacitance is kept under a current density of 20 A g−1, and no arresting degradation is observed for capacitance after 5000 times cycle, perfectly demonstrating the excellent cycle and rate capability of the obtained material. The obtained N and O co-doped hierarchical porous carbon are expected to be an ideal substitution for commercial activated carbon.
- Published
- 2020
33. Tag-Free Site-Specific BMP-2 Immobilization with Long-Acting Bioactivities via a Simple Sugar–Lectin Interaction
- Author
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Linyong Zhu, Chunyan Bao, Shaoliang Lin, Honglei Wang, Changsheng Liu, Joachim Kohn, Zheng Zhang, Xue Qu, Haoqi Tan, and Miao Lei
- Subjects
Glycosylation ,medicine.medical_treatment ,0206 medical engineering ,Biomedical Engineering ,Bone Morphogenetic Protein 2 ,Biocompatible Materials ,02 engineering and technology ,Bone morphogenetic protein 2 ,law.invention ,Biomaterials ,chemistry.chemical_compound ,law ,Lectins ,Concanavalin A ,medicine ,Animals ,chemistry.chemical_classification ,biology ,Chemistry ,Growth factor ,Monosaccharides ,Lectin ,Oligosaccharide ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Recombinant Proteins ,Biochemistry ,biology.protein ,Recombinant DNA ,0210 nano-technology ,Glycoprotein - Abstract
The construction of a biomaterial matrix with biological properties is of great importance to developing functional materials for clinical use. However, the site-specific immobilization of growth factors to endow materials with bioactivities has been a challenge to date. Considering the wide existence of glycosylation in mammalian proteins or recombinant proteins, we establish a bioaffinity-based protein immobilization strategy (bioanchoring method) utilizing the native sugar-lectin interaction between concanavalin A (Con A) and the oligosaccharide chain on glycosylated bone morphogenetic protein-2 (GBMP-2). The interaction realizes the site-specific immobilization of GBMP-2 to a substrate modified with Con A while preserving its bioactivity in a sustained and highly efficient way, as evidenced by its enhanced ability to induce osteodifferentiation compared with that of the soluble GBMP-2. Moreover, the surface with Con A-bioanchored GBMP-2 can be reused to stimulate multiple batches of C2C12 cells to differentiate almost to the same degree. Even after 4 month storage at 4 °C in phosphate-buffered saline (PBS), the Con A-bioanchored GBMP-2 still maintains the bioactivity to stimulate the differentiation of C2C12 cells. Furthermore, the ectopic ossification test proves the in vivo bioactivity of bioanchored GBMP-2. Overall, our results demonstrate that the tag-free and site (i.e., sugar chain)-specific protein immobilization strategy represents a simple and generic alternative, which is promising to apply for other glycoprotein immobilization and application. It should be noted that although the lectin we utilized can only bind to d-mannose/d-glucose, the diversity of the lectin family assures that a specific lectin could be offered for other sugar types, thus expanding the applicable scope further.
- Published
- 2020
34. Redox-Channeling Polydopamine-Ferrocene (PDA-Fc) Coating To Confer Context-Dependent and Photothermal Antimicrobial Activities
- Author
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Changsheng Liu, Zhanyi Chen, Artem A. Antoshin, Xue Qu, Gregory F. Payne, Haoqi Tan, Jialin Song, Huan Liu, and Miao Lei
- Subjects
Male ,Indoles ,Metallocenes ,Polymers ,02 engineering and technology ,Microscopy, Atomic Force ,medicine.disease_cause ,01 natural sciences ,Rats, Sprague-Dawley ,Mice ,Coated Materials, Biocompatible ,X-Ray Diffraction ,General Materials Science ,Titanium ,Nanotubes ,biology ,Photothermal effect ,Temperature ,3T3 Cells ,Prostheses and Implants ,021001 nanoscience & nanotechnology ,Antimicrobial ,Anti-Bacterial Agents ,0210 nano-technology ,Oxidation-Reduction ,Staphylococcus aureus ,Materials science ,education ,Context (language use) ,Microbial Sensitivity Tests ,010402 general chemistry ,Microscopy, Electron, Transmission ,Escherichia coli ,medicine ,Animals ,Ferrous Compounds ,Wound Healing ,Biofilm ,Phototherapy ,Photothermal therapy ,biology.organism_classification ,Rats ,0104 chemical sciences ,Biofilms ,Microscopy, Electron, Scanning ,Biophysics ,Surface modification ,Reactive Oxygen Species ,Bacteria - Abstract
Microbial disinfection associated with medical device surfaces has been an increasing need, and surface modification strategies such as antibacterial coatings have gained great interest. Here, we report the development of polydopamine-ferrocene (PDA-Fc)-functionalized TiO2 nanorods (Ti-Nd-PDA-Fc) as a context-dependent antibacterial system on implant to combat bacterial infection and hinder biofilm formation. In this work, two synergistic antimicrobial mechanisms of the PDA-Fc coating are proposed. First, the PDA-Fc coating is redox-active and can be locally activated to release antibacterial reactive oxygen species (ROS), especially ·OH in response to the acidic microenvironment induced by bacteria colonization and host immune responses. The results demonstrate that redox-based antimicrobial activity of Ti-Nd-PDA-Fc offers antibacterial efficacy of over 95 and 92% against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli), respectively. Second, the photothermal effect of PDA can enhance the antibacterial capability upon near-infrared (NIR) irradiation, with over 99% killing efficacy against MRSA and E. coli, and even suppress the formation of biofilm through both localized hyperthermia and enhanced ·OH generation. Additionally, Ti-Nd-PDA-Fc is biocompatible when tested with model pre-osteoblast MC-3T3 E1 cells and promotes cell adhesion and spreading presumably due to its nanotopographical features. The MRSA-infected wound model also indicates that Ti-Nd-PDA-Fc with NIR irradiation can effectively eliminate bacterial infection and suppress host inflammatory responses. We believe that this study demonstrates a simple means to create biocompatible redox-active coatings that confer context-dependent antibacterial activities to implant surfaces.
- Published
- 2020
35. A novel MEF2C mutation in lymphoid neoplasm diffuse large B-cell lymphoma promotes tumorigenesis by increasing c-JUN expression
- Author
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Zhao Jingjing, Yuan Chunluan, Miao Lei, Cao Sha, Hu Yapeng, Zhang Weimin, and Zhang Jie
- Subjects
0301 basic medicine ,Proto-Oncogene Proteins c-jun ,Mice, Nude ,Biology ,Malignancy ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,immune system diseases ,hemic and lymphatic diseases ,Cell Adhesion ,medicine ,Animals ,Humans ,Cell Proliferation ,Pharmacology ,Mutation ,MEF2 Transcription Factors ,Genetic heterogeneity ,Point mutation ,c-jun ,General Medicine ,medicine.disease ,Tumor Burden ,Up-Regulation ,Lymphoma ,Gene Expression Regulation, Neoplastic ,HEK293 Cells ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Lymphoma, Large B-Cell, Diffuse ,Carcinogenesis ,Diffuse large B-cell lymphoma ,Signal Transduction - Abstract
Diffuse large B-cell lymphoma (DLBCL) is the most aggressive non-Hodgkin lymphoma (NHL), accounting for about 31% of the newly diagnosed NHL worldwide. Although approximately 60% of patients who initially received a standard R-CHOP treatment likely have a 3-year event-free survival, many patients become refractory or relapsed due to the genetic heterogeneity of this malignancy. Hence, new treatment strategies are urgently needed. MEF2C, a member of the MEF2 transcription factor family gene, plays great important roles involved in the development of various tissues and the pathogenesis of lymphoma. However, the exact functions and molecular mechanisms of MEF2C in DLBCL are not fully investigated. By Sanger sequencing, we identified a novel point mutation of MEF2C at the p.N389 site in DLBCL patient, which was further validated by several DLBCL cell lines. Intriguingly, we found that the p.N389S mutation did not influence MEF2C expression, protein stability, and subcellular distribution, but enhanced its transcriptional activity. Furthermore, we demonstrated that MEF2C p.N389S mutation promotes DLBCL cell proliferation, cellular adhesion, and tumor formation in nude mice. On mechanism, our data revealed that MEF2C p.N389S mutation increases c-JUN expression, and c-JUN regulation mediated the oncogenic function of MEF2C p.N389S mutation on DLBCL cells. Our finding may provide a significant insight into the DLBCL and a compelling therapy target for this disease treatment.
- Published
- 2020
36. Diselenide-crosslinked zwitterionic nanogels with dual redox-labile properties for controlled drug release
- Author
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Feifei An, Yefei Tian, Luke Yan, and Miao Lei
- Subjects
chemistry.chemical_classification ,Polymers and Plastics ,Chemistry ,Phosphorylcholine ,Organic Chemistry ,technology, industry, and agriculture ,Bioengineering ,Polymer ,Biochemistry ,Controlled release ,Combinatorial chemistry ,Diselenide ,Drug delivery ,Precipitation polymerization ,Copolymer ,Nanogel - Abstract
Drug delivery systems developed from zwitterionic polymers have intriguing antifouling properties. Combining these with stimuli-responsiveness, we fabricated a new type of zwitterionic nanogel with dual redox-labile properties by copolymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and a diselenide bond-containing crosslinker through reflux precipitation polymerization. Poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)-based nanogels show high protein adsorption resistibility and colloidal stability at high salt concentrations. By incorporation of diselenide bonds into polymer networks, the nanogels are endowed with unique dual redox-labile properties that lead to their efficient degradation into short polymers either in a reducing environment (GSH) or in an oxidative environment (H2O2). Nanogels loaded with anticancer drugs (doxorubicin, DOX) display a finely controlled release behavior and low leakage of DOX under physiological conditions (15.0% in 24 h), but rapid and sufficient release under reducing conditions (88.3% in 24 h) or under oxidative conditions (82.2% in 24 h). Cell viability assays reveal that the blank nanogels have no cytotoxicity within a wide concentration range, while DOX-loaded nanogels present a significant inhibitive effect against tumor cells.
- Published
- 2020
37. A multifunctional CuSCN interlayer in carbon electrode-based CsPbIBr2 all-inorganic perovskite solar cells for boosting the efficiency and stability
- Author
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Miao Lei, Wei Zhang, Guiqiang Wang, and Jieqiong Liu
- Subjects
Fuel Technology ,Materials science ,Fabrication ,Chemical engineering ,Renewable Energy, Sustainability and the Environment ,Energy conversion efficiency ,Electrode ,Energy Engineering and Power Technology ,Carbon layer ,Surface energy - Abstract
Carbon electrode-based all-inorganic perovskite solar cells (PSCs) without hole-transport materials are attracting extensive interest due to their low cost, simple fabrication process, and high stability. Nevertheless, the conversion efficiency of carbon electrode-based PSCs is far from satisfactory owing to serious charge recombination at the inorganic perovskite/carbon interface, which mainly derives from the mismatched energy level between the inorganic perovskite film and carbon layer. Herein, a hydrophobic CuSCN film is introduced into carbon electrode-based CsPbIBr2 all-inorganic PSCs as a multifunctional interlayer between the CsPbIBr2 perovskite film and carbon electrode to form a favorable interfacial energy level alignment and protect the CsPbIBr2 perovskite from ambient moisture. It is found that introducing a CuSCN interlayer can not only enhance the hole extraction and suppress the charge recombination in carbon electrode-based CsPbIBr2 all-inorganic PSCs, but also improve the stability of the cell. Moreover, the very strong interaction between SCN−1 and Pb2+ remarkably reduces the surface defects of the CsPbIBr2 perovskite film. Consequently, the device with the CuSCN interlayer displays an improved power conversion efficiency of 7.30% in comparison with 5.19% for the device without the CuSCN interlayer and an excellent long-term stability under the ambient conditions.
- Published
- 2020
38. LncRNA HCP5 promotes LAML progression via PSMB8-mediated PI3K/AKT pathway activation
- Author
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Zhao Jingjing, Zhu Yuanxin, Mao Jianping, Miao Lei, Wei Jifeng, Wang Ying, Jia Tao, Xue Lianguo, and Zhao Lidong
- Subjects
Adult ,Male ,0301 basic medicine ,Proteasome Endopeptidase Complex ,Phosphatidylinositol 3-Kinases ,03 medical and health sciences ,0302 clinical medicine ,hemic and lymphatic diseases ,Humans ,Medicine ,PI3K/AKT/mTOR pathway ,Aged ,Cell Proliferation ,Aged, 80 and over ,Pharmacology ,Gene knockdown ,business.industry ,Myeloid leukemia ,PSMB8 ,General Medicine ,Middle Aged ,Long non-coding RNA ,Leukemia, Myeloid, Acute ,030104 developmental biology ,Proteasome ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Biomarker (medicine) ,Female ,RNA, Long Noncoding ,business ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Acute myeloid leukemia is an aggressive myeloid malignancy, characterized by rapid cellular proliferation and generally high mortality. Due to the lack of a complete understanding of AML, its clinical outcomes are still not satisfactory. In this study, we examined the function of the long non-coding RNA-HLA complex P5 (HCP5) on AML by analyzing the clinical samples, TCGA data, and by shRNA-mediated HCP5 deficiency in vitro. Our results showed that HCP5 is highly expressed in AML and is positive associated with poor prognosis, and HCP5 knockdown was significantly suppressing AML cell line proliferation and inducing G1/S arrest in vitro. In mechanism, the proteasome subunit beta type 8 (PSMB8) expression was dramatically inhibited in HCP5 knockdown cells while increased in HCP5 overexpression cells. PSMB8 was also highly expressed in AML and with poor prognosis. Furthermore, HCP5 regulates PI3K/AKT pathway activation depending on PSMB8. Our results showed a promoting function of HCP5 on AML and may provide a compelling biomarker and therapy target for AML.
- Published
- 2019
39. Research progress in 3D printing technology for carbon nanotubes composites
- Author
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LU Hao, LI Nan, WANG Hai-bo, LIAO Bang-quan, JIANG Ya-ming, JING Miao-lei, XU Zhi-wei, CHEN Li, and ZHANG Xing-xiang
- Subjects
mechanical property ,carbon nanotubes ,3d printing ,polymer composites ,electrical property ,lcsh:TA401-492 ,lcsh:Materials of engineering and construction. Mechanics of materials - Abstract
3D printing technology is one of the additive manufacturing technologies for rapid process and manufacture of complex geometric components. Based on the three-dimensional data model, the material is accumulated layer by layer controlled by computer, and eventually forms the solid object. Compared to the traditional manufacturing methods, 3D printing technology has many advantages, such as time saving, low cost, easy operation, no mold and strong controllability of component geometry, etc. Along with the development of this technology, several types of 3D printing technologies, such as fused deposition modeling, selective laser sintering, stereo lithography, digital light processing and solvent casting molding, have been produced according to the core of the printing technology, applicable materials and equipment composition. The principles and characteristics of the four most representative 3D printing forming processes were introduced in this paper, and the research progress of carbon nanotubes to reinforce polymer composites with different types of 3D printing forming processes applied in recent years was summarized. At the same time, it was predicted that the 3D printing molding technology will be developed towards the direction of high precision, industrialization, popularization and high integration in this field, and the research and development of 3D printing materials will also have more prospects.
- Published
- 2019
40. Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment
- Author
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Xiaoli Liu, Miao Lei, Xue Qu, Changsheng Liu, Shijia Wang, and Junjie Sun
- Subjects
Chronic wound ,Staphylococcus aureus ,Materials science ,Alginates ,Cell Survival ,Metal ions in aqueous solution ,Biomedical Engineering ,Biophysics ,chemistry.chemical_element ,Bioengineering ,Biomaterials ,Mice ,Alginate dressing ,Anti-Infective Agents ,Escherichia coli ,Human Umbilical Vein Endothelial Cells ,Medical technology ,medicine ,Animals ,Humans ,R855-855.5 ,Materials of engineering and construction. Mechanics of materials ,Antimicrobial properties of copper ,Electrochemical Techniques ,Biomaterials Synthesis and Characterization ,Bandages ,Copper ,Controlled release ,Anode ,chemistry ,Chemical engineering ,Wound Infection ,TA401-492 ,medicine.symptom ,Antibacterial activity - Abstract
The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing. There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions. Alginate hydrogel is a common dressing for wound treatment. Copper is one of the trace elements in human body with inherent antibacterial activity. Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions. In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode. The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition. In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities. Film’s cytocompatibility is well controlled by the imposed charges for Cu2+-Alg fabrication. The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses. We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment. More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.
- Published
- 2021
41. Characteristics of airborne bacterial communities and antibiotic resistance genes under different air quality levels
- Author
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Xu Yan, Jiahui Ma, Xinqing Chen, Miao Lei, Tianning Li, and Yunping Han
- Subjects
Air quality levels ,Bacteria ,Redundancy analysis ,Air Microbiology ,Drug Resistance, Microbial ,Anti-Bacterial Agents ,Airborne antibiotic resistance genes ,Environmental sciences ,Genes, Bacterial ,Pathogenic bacteria ,Air Pollution ,Airborne bacterial community ,Humans ,GE1-350 ,General Environmental Science ,Bioaerosol ,Environmental Monitoring - Abstract
Pathogenic bacteria and antibiotic resistance genes (ARGs) in bioaerosols are major threats to human health. In this study, the microbial community structure and ARG distribution characteristics of airborne bacteria in total suspended particulates (TSP) and PM2.5 were investigated under different air quality levels in Xinxiang, Central China. The results revealed that with the deterioration of air quality, the concentrations of airborne bacteria in both TSP and PM2.5 decreased; however, the relative amounts of pathogenic bacteria increased. The predominant genera in pathogenic bacteria of Bacillus, Sphingomonas, Corynebacterium, Rhodococcus, and Staphylococcus were identified in both TSP and PM2.5. Although the airborne bacteria concentrations and absolute abundances of ARGs in TSP were higher than those in PM2.5 under identical air quality conditions, the bacterial community structure and relative amounts of pathogenic bacteria were similar. In addition, the relationship between environmental factors of ions, metal elements, and meteorological parameters and the community structures of airborne bacteria and pathogenic bacteria were also analyzed. The effects of soluble ions and metal elements on several dominant genera of total bacteria and pathogenic bacteria differed, probably due to the strong tolerance of pathogenic bacteria to harsh atmospheric environments Different subtypes of ARGs showed various distribution characteristics with variations in air quality. The deterioration of air quality can inhibit the dissemination of ARGs, as the minimum values of all ARGs and class 1 integrase intI1 were observed under Severely Polluted conditions. This study provides a comprehensive understanding of the effect of air pollution levels on the airborne bacteria community composition and ARG distribution.
- Published
- 2021
42. Single Step Assembly of Janus Porous Biomaterial by Sub‐Ambient Temperature Electrodeposition (Small 48/2022)
- Author
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Miao Lei, Haitao Liao, Shijia Wang, Hang Zhou, Zhiling Zhao, Gregory F. Payne, Xue Qu, and Changsheng Liu
- Subjects
Biomaterials ,General Materials Science ,General Chemistry ,Biotechnology - Published
- 2022
43. Single Step Assembly of Janus Porous Biomaterial by Sub‐Ambient Temperature Electrodeposition
- Author
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Miao Lei, Haitao Liao, Shijia Wang, Hang Zhou, Zhiling Zhao, Gregory F. Payne, Xue Qu, and Changsheng Liu
- Subjects
Biomaterials ,Temperature ,Animals ,Biocompatible Materials ,General Materials Science ,General Chemistry ,Regenerative Medicine ,Porosity ,Electroplating ,Rats ,Biotechnology - Abstract
Janus porous biomaterials are gaining increasing attention and there are considerable efforts to develop simple, rapid, and scalable methods capable of tuning micro- and macro-structures. Here, a single-step electro-fabrication method to create a Janus porous film by the electrodeposition of the amino-polysaccharide chitosan is reported. Specifically, a Janus structure emerges spontaneously when electrodeposition is performed at sub-ambient temperature (0-5 °C). Sub-ambient temperature electrodeposition experiments show that: a Janus microstructure emerges (potentially as the result of a subtle alteration of the intermolecular interactions responsible for self-assembly); important microstructural features (pore size, porosity, and thicknesses) can be tuned by conditions; and this method is readily scalable (vs serial printing) and can yield complex tubular structures with Janus faces. In vitro studies demonstrate anisotropic cell guidance, and in vivo studies using a rat calvarial defect model further confirm the beneficial features of such Janus porous film for guided bone regeneration. In summary, these results further demonstrate that electro-fabrication provides a simple and scalable platform technology for the controlled functional structures of soft matter for applications in regenerative medicine.
- Published
- 2022
44. Fenton Reaction Induced by Fe-Based Nanoparticles for Tumor Therapy
- Author
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Jian Zhou, Lu-Ke Yan, Xue-Liang Peng, Miao Lei, and Dai-Xu Wei
- Subjects
Fenton reaction ,Tumor microenvironment ,Chemistry ,Iron ,Biomedical Engineering ,Cancer therapy ,Pharmaceutical Science ,Medicine (miscellaneous) ,Tumor therapy ,Nanoparticle ,Bioengineering ,Hydrogen Peroxide ,Combinatorial chemistry ,chemistry.chemical_compound ,Neoplasms ,Cancer cell ,Tumor Microenvironment ,Humans ,Nanoparticles ,General Materials Science ,Fe based ,Hydrogen peroxide - Abstract
Fenton reaction, a typical inorganic reaction, is broadly utilized in the field of wastewater treatment. Recently In case of its ability to inhibit the growth of cancer cells, it has been frequently reported in cancer treatment. Using the unique tumor microenvironment in cancer cells, many iron-based nanoparticles have been developed to release iron ions in cancer cells to induce Fenton reaction. In this mini review, we outline several different types of iron-based nanoparticles and several main means to enhance Fenton reaction in cancer cells. Finally, we discussed the advantages and disadvantages of iron-based nanoparticles for cancer therapy, prospected the future development of iron-based nanoparticles. It is believed that iron-based nanoparticles can make certain contribution to the cause of human cancer in the future.
- Published
- 2021
45. Concentrations, Size Distribution, and Community Structure Characteristics of Culturable Airborne Antibiotic-Resistant Bacteria in Xinxiang, Central China
- Author
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Miao Lei, Chun Chen, Dezhi Qiu, Yunping Han, Xinqing Chen, Mengjia Cui, Xu Yan, Tianning Li, Jingyuan Ren, Li Guo, and Jiahui Ma
- Subjects
Atmospheric Science ,Veterinary medicine ,biology ,Macrococcus ,atmospheric pollution ,distribution characteristic ,Indoor bioaerosol ,culturable airborne bacteria ,Micrococcus ,Bacillus ,pathogenic ,opportunistic pathogenic bacteria ,Environmental Science (miscellaneous) ,biology.organism_classification ,Antibiotic resistance ,Enterococcus ,Meteorology. Climatology ,health risk assessment ,Stenotrophomonas ,QC851-999 ,antibiotic-resistance bacteria ,community structure ,Bacteria - Abstract
Antimicrobial resistance is considered an important threat to global health and has recently attracted significant attention from the public. In this study, the concentrations and size distribution characteristics of culturable airborne total bacteria (TB) and four antibiotic-resistant bacteria (tetracycline-resistant bacteria (TRB), ciprofloxacin-resistant bacteria (CRB), erythromycin-resistant bacteria (ERB), and ampicillin-resistant bacteria (ARB)) were investigated for approximately one year to explore their variations under different seasons, diurnal periods, and air quality levels. The concentrations of TB and four antibiotic-resistant bacteria in winter and night were higher than during other seasons and diurnal periods. Their maximum concentrations were detected from air under moderate pollution or heavy pollution. PM2.5, PM10, SO2, and NO2 were positively related to TB and four antibiotic-resistant bacteria (p <, 0.01), whereas O3 and wind speed were negatively related to them (p <, 0.05). The particle size of TB and four antibiotic-resistant bacteria were mainly distributed in stage V (1.1–2.2 µm). Bacillus was the dominant genus of ARB (75.97%) and CRB (25.67%). Staphylococcus and Macrococcus were the dominant genera of TRB (46.05%) and ERB (47.67%), respectively. The opportunistic pathogens of Micrococcus, Sphingomonas, Enterococcus, Rhodococcus, and Stenotrophomonas were also identified. This study provides important references for understanding the threat of bioaerosols to human health.
- Published
- 2021
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46. Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate
- Author
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Gang Liu, Fangcheng Jiang, Qingrong Huang, Dengfeng Peng, Weiping Jin, Bei Zhang, Wenjing Huang, and Miao Lei
- Subjects
Whey protein ,Health (social science) ,nanoemulsion ,TP1-1185 ,02 engineering and technology ,Plant Science ,Health Professions (miscellaneous) ,Microbiology ,Article ,Whey protein isolate ,symbols.namesake ,chemistry.chemical_compound ,0404 agricultural biotechnology ,conjugate ,casticin ,Lactose ,whey protein isolated ,Chromatography ,biology ,Chemistry ,Chemical technology ,Pinocytosis ,cellular uptake ,04 agricultural and veterinary sciences ,021001 nanoscience & nanotechnology ,040401 food science ,Maillard reaction ,Cancer cell ,symbols ,Casticin ,biology.protein ,0210 nano-technology ,Food Science ,Conjugate - Abstract
Casticin has wide-ranging functional activities, but its water solubility is poor in food products. Here, a nanoemulsion stabilized by Maillard whey protein isolate conjugates (MWPI) was fabricated to encapsulate casticin. The nanoemulsion, with an average diameter of 200 nm, possessed the capability to load 700 μg/g casticin. MWPI-stabilized nanoemulsion showed better stability than that of the WPI nanoemulsion during 4 weeks of storage. Both the inhibition effects of the casticin-loaded nanoemulsion on cancer cells and the process of cellular uptake were studied. Results revealed that the casticin-loaded nanoemulsion had better inhibitory activity in HepG2 and MCF-7 cells than free casticin. Cellular uptake of the nanoemulsion displayed a time-dependent manner. After the nanoemulsion passed into HepG2 and MCF-7 cells, it would locate in the lysosome but not in the nucleus. The main pathway for the nanoemulsion to enter HepG2 cells was pinocytosis, whereas, it entered MCF-7 predominantly through the clathrin-mediated pit. This work implies that MWPI-stabilized nanoemulsions could be utilized as an effective delivery system to load casticin and have the potential to be applied in the food and pharmaceutical industries.
- Published
- 2021
47. A novel way to facilely degrade organic pollutants with the tail-gas derived from PHP (phosphoric acid plus hydrogen peroxide) pretreatment of lignocellulose
- Author
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Li Zhao, Fei Shen, Miao Lei, Jinguang Hu, Jianmei Zou, Mei Huang, Shihuai Deng, Dong Tian, Yongmei Zeng, and Gang Yang
- Subjects
Pollutant ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Resorcinol ,Hydrogen Peroxide ,Pollution ,Lignin ,chemistry.chemical_compound ,chemistry ,Methyl orange ,Rhodamine B ,Environmental Chemistry ,Degradation (geology) ,Environmental Pollutants ,Phosphoric Acids ,Malachite green ,Hydrogen peroxide ,Waste Management and Disposal ,Phosphoric acid ,Nuclear chemistry - Abstract
The abundantly released tail-gas from lignocellulose pretreatment with phosphoric acid plus hydrogen peroxide (PHP) was found to accelerate the aging of latex/silicone textural accessories of the pretreatment device. Inspired by this, tail-gas was utilized to control organic pollutants. Methylene blue (MB), as a model pollutant, was rapidly decolorized by the tail-gas, and oxidative degradation was substantially proven by full-wavelength scanning with a UV–visible spectrometer. The tail-gas from six typical lignocellulosic feedstocks produced 68.0–98.3% MB degradation, suggesting its wide feedstock compatibility. Three other dyes, including rhodamine B, methyl orange and malachite green, obtained 97.5–99.5% degradation; moreover, tetracycline, resorcinol and hexachlorobenzene achieved 73.8–93.7% degradation, suggesting a superior pollutant compatibility. In a cytotoxicity assessment, the survival rate of the degraded MB was 103.5% compared with 80.4% for the untreated MB, implying almost no cytotoxicity after MB degradation. Mechanism investigations indicated that the self-exothermic reaction in PHP pretreatment drove the self-generated peroxy acids into tail-gas. Moreover, it heated the pollutant solution and thermally activated peroxy acids as free radicals for efficient pollutant degradation. Here, a brand-new technique for degrading organic pollutants with a “Win–Win–Win” concept was purposed for lignocellulose valorization, pollutant control by waste tail-gas, and biofuel production.
- Published
- 2021
48. Spatio-temporal dynamics in soil water storage reveals effects of nitrogen inputs on soil water consumption at different growth stages of winter wheat
- Author
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Yan Deng, Jian-Fu Xue, Ren Aixia, Zhenping Yang, Wen Lin, Ling-zhu Xue, Zhiqiang Gao, Pei-ru Wang, Miao-miao Lei, and Min Sun
- Subjects
0208 environmental biotechnology ,Soil Science ,04 agricultural and veterinary sciences ,02 engineering and technology ,Plastic mulch ,020801 environmental engineering ,Summer fallow ,Agronomy ,Evapotranspiration ,Soil water ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Soil horizon ,Environmental science ,Water-use efficiency ,Agronomy and Crop Science ,Mulch ,Water use ,Earth-Surface Processes ,Water Science and Technology - Abstract
Water availability is a major constraint for wheat production in the southeast of the Loess Plateau, China. Plastic mulch exhibits greater potential in rainwater harvesting while nitrogen (N) fertilizer has a strong effect on soil water utilization. Most of annual precipitation in the Loess Plateau occurs in summer. Rainwater retention in summer fallow becomes very important for winter wheat production in the dryland of the Loess Plateau. Straw mulching in summer fallow are commonly used to prevent water evaporation from soil surface and retain rainwater. Recently plastic mulching has also been used. Nitrogen inputs have also a significant impact on soil water utilization and yield production. However, there is little information available for the effects of full coverage of plots with plastic mulch (PM) in summer fallow on soil water storage at sowing and N rates interact with the soil water utilization in winter wheat yield, water use efficiency (WUE) and grain protein concentration (GPC). Therefore, field experiments were conducted in three consecutive years with variable rainfall. The results showed that PM in summer fallow increased soil water storage at sowing by at least 50 mm, and water storage efficiency was the highest (56.5%) in the dry season. Spatio-temporal dynamics in the soil water storage of 0–300 cm depth exhibited a downward movement with crop development. Approximately half of soil water consumption from flowering to maturity was derived from the 200–300 cm soil layers. The optimal N rate with PM increased grain yield by 27% in the wet season, 36% in the normal season, and 41% in the dry season. PM in combination with optimized N could improve WUE only when annual rainfall is equal to or above the yearly average. Furthermore, evapotranspiration is negatively correlated with GPC although the soil water consumption from 200 to 300 cm depth is positively correlated with post-anthesis N uptake. This suggests that the additional N is needed to further increase GPC. In the season with soil water storage of 550–600 mm at sowing, the N rate of 225 kg ha−1 with PM could achieve the highest yield and 13.2% of GPC. In the season with soil water storage of 450–550 mm at sowing, the N rate of 150 kg ha−1with PM could achieve the highest yield, and 14.3% of GPC. When the season with soil water storage of approximately 400 mm, the N rate of 75 kg ha−1 with PM achieved the highest yield, and 15.0% of GPC. Our results indicate that adjusting N fertilizer inputs based on seasonal variation in summer rainfall could enhance wheat yield and GPC in the southeast region of the Loess Plateau.
- Published
- 2019
49. Circulating solution for the degumming and modification of hemp fiber by the laccase–2,2,6,6-tetramethylpiperidine-1-oxyl radical–hemicellulase system
- Author
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Jing Miao-lei, Liu Liu, Fang Bin, Xiang Yeping, Yu Jianyong, Zhang Ruiyun, and Ji Feng
- Subjects
Laccase ,010407 polymers ,Materials science ,Polymers and Plastics ,Hemp fiber ,One-Step ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,2 2 6 6 tetramethylpiperidine 1 oxyl ,Chemical engineering ,Chemical Engineering (miscellaneous) ,Textile fiber ,0210 nano-technology - Abstract
Hemp fiber, as a kind of renewable textile fiber, has evoked much interest due to its excellent properties in various fields. It has been investigated in just one step to finish the process of chemo-enzymatic degumming and modification of hemp fiber, which is done in acetic acidsodium acetate buffer solution containing laccase (6.0 g), hemicellulase (0.3 g) and 2,2,6,6-tetramethylpiperidine-1-oxyl radical (0.3 g) under a mild condition (pH 5, 50C). The degumming effects of different batches of raw hemp fibers were also discussed. We found that the residual rate, fineness, length and tensile strength of degummed fiber were 15–27%, 9.00–24.00 dtex, 14.00–19.00 cm and 3.8–5.1 cN/dtex, respectively. Meanwhile, some of the oxidized functional groups were introduced into degummed fiber, which improved the moisture absorption and water retention value of the hemp fiber. The hemp fiber treated by this process could be further improved and then applied in the textile industry.
- Published
- 2019
50. Optimization of sowing date and seeding rate for high winter wheat yield based on pre-winter plant development and soil water usage in the Loess Plateau, China
- Author
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Pei-ru Wang, Ling-zhu Xue, Jian-Fu Xue, Ren Aixia, Zhenping Yang, Zhiqiang Gao, Min Sun, and Miao-miao Lei
- Subjects
0106 biological sciences ,dry-land ,accumulated temperature ,Agriculture (General) ,Field experiment ,sowing date ,soil water ,Growing season ,Plant Science ,seeding rate ,01 natural sciences ,Biochemistry ,S1-972 ,Summer fallow ,Food Animals ,Anthesis ,Ecology ,Sowing ,04 agricultural and veterinary sciences ,winter wheat ,Agronomy ,Soil water ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Dormancy ,Environmental science ,Animal Science and Zoology ,Seeding ,Agronomy and Crop Science ,010606 plant biology & botany ,Food Science - Abstract
Sowing date and seeding rate are critical for productivity of winter wheat (Triticum aestivum L.). A three-year field experiment was conducted with three sowing dates (20 September (SD1), 1 October (SD2), and 10 October (SD3)) and three seeding rates (SR67.5, SR90, and SR112.5) to determine suitable sowing date and seeding rate for high wheat yield. A large seasonal variation in accumulated temperature from sowing to winter dormancy was observed among three growing seasons. Suitable sowing dates for strong seedlings before winter varied with the seasons, that was SD2 in 2012–2013, SD3 in 2013–2014, and SD2 as well as SD1 in 2014–2015. Seasonal variation in precipitation during summer fallow also had substantial effects on soil water storage, and consequently influenced grain yield through soil water consumption from winter dormancy to maturity stages. Lower consumption of soil water from winter dormancy to booting stages could make more water available for productive growth from anthesis to maturity stages, leading to higher grain yield. SD2 combined with SR90 had the lowest soil water consumption from winter dormancy to booting stages in 2012–2013 and 2014–2015; while in 2013–2014, it was close to that with SR67.5 or SR112.5. For productive growth from anthesis to maturity stages, SD2 with SR90 had the highest soil water consumption in all three seasons. The highest water consumption in the productive growth period resulted in the best grain yield in both low and high rainfall years. Ear number largely contributed to the seasonal variation in grain yield, while grain number per ear and 1 000-grain weight also contributed to grain yield, especially when soil water storage was high. Our results indicate that sowing date and seeding rate affect grain yield through seedling development before winter and also affect soil water consumption in different growth periods. By selecting the suitable sowing date (1 October) in combination with the proper seeding rate of 90 kg ha–1, the best yield was achieved. Based on these results, we recommend that the current sowing date be delayed from 22 or 23 September to 1 October.
- Published
- 2019
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