1. Blood-derived integration-free iPS cell line UKBi011-A from a diagnosed male Alzheimer's disease patient with APOE ɛ4/ɛ4 genotype
- Author
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Klaus Fliessbach, Oliver Brüstle, Catrin Cornelia Thiele, Monika Veltel, Michael Peitz, Alfredo Ramirez, Melanie Bloschies, and Tamara Bechler
- Subjects
Male ,0301 basic medicine ,Apolipoprotein E4 ,metabolism [Blood Cells] ,genetics [Alzheimer Disease] ,pathology [Alzheimer Disease] ,0302 clinical medicine ,neurodegenerative disease ,Genotype ,iPS cell line ,Cellular Reprogramming Techniques ,Vector (molecular biology) ,Induced pluripotent stem cell ,lcsh:QH301-705.5 ,genetics [Apolipoprotein E4] ,Aged, 80 and over ,diagnosis [Alzheimer Disease] ,General Medicine ,genetics [Transcription Factors] ,Alzheimer's disease ,pathology [Induced Pluripotent Stem Cells] ,3. Good health ,metabolism [Induced Pluripotent Stem Cells] ,pathology [Blood Cells] ,KLF4 ,embryonic structures ,Reprogramming ,metabolism [Alzheimer Disease] ,APOE ,Induced Pluripotent Stem Cells ,Biology ,Polymorphism, Single Nucleotide ,biosynthesis [Transcription Factors] ,Kruppel-Like Factor 4 ,03 medical and health sciences ,metabolism [Apolipoprotein E4] ,SOX2 ,Alzheimer Disease ,ddc:570 ,Humans ,SNP ,Allele ,Blood Cells ,Cell Biology ,030104 developmental biology ,lcsh:Biology (General) ,genotyping ,Immunology ,030217 neurology & neurosurgery ,Transcription Factors ,Developmental Biology ,dementia - Abstract
Alzheimer's disease (AD) is most the frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for late-onset AD. Here, we present an iPSC line generated from peripheral blood cells of a male AD patient employing Sendai virus vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The characterized iPSC line expresses typical human pluripotency markers and shows differentiation into all three germ layers, complete reprogramming vector clearance, a normal SNP genotype and maintenance of the APOE ε4/ε4 allele.
- Published
- 2018
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