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We characterized the world’s second case with ascertained extreme resilience to autosomal dominant Alzheimer’s disease (ADAD). Side-by-side comparisons of this male case and the previously reported female case with ADAD homozygote for the APOE3 Christchurch (APOECh) variant allowed us to discern common features. The male remained cognitively intact until 67 years of age despite carrying a PSEN1-E280A mutation. Like the APOECh carrier, he had extremely elevated amyloid plaque burden and limited entorhinal Tau tangle burden. He did not carry the APOECh variant but was heterozygous for a rare variant in RELN (H3447R, termed COLBOS after the Colombia–Boston biomarker research study), a ligand that like apolipoprotein E binds to the VLDLr and APOEr2 receptors. RELN-COLBOS is a gain-of-function variant showing stronger ability to activate its canonical protein target Dab1 and reduce human Tau phosphorylation in a knockin mouse. A genetic variant in a case protected from ADAD suggests a role for RELN signaling in resilience to dementia.

Tendo como desafio ampliar as relações da universidade com a comunidade, o curso de graduação em Jornalismo passou a incluir atividades que propõem o protagonismo dos alunos. Na disciplina Oficina de Jornalismo em Comunidades, do curso de Jornalismo da Universidade do Vale do Taquari (Univates), os estudantes foram convidados a realizar intervenções comunitárias junto a quatro grupos sociais, usando a linguagem audiovisual para abordar temas ambientais. O objetivo do estudo é analisar a potência das práticas de jornalismo comunitário para fazerem emergir gestos de criação. Problematiza-se como se dinamizaram as intervenções nos diferentes grupos sociais, como se caracterizam as abordagens dadas aos temas ambientais nos audiovisuais e que aproximações e distanciamentos observam-se em relação aos modos de fazer que emergiram. Trata-se de um estudo qualitativo, exploratório, descritivo e de cunho intervencionista. With the challenge of expanding the university’s relations with the community, the undergraduate course in Journalism started to include activities that propose the students’ prota-gonism. In the course called Workshop on Journalism in Communities, part of the Journalism Program at the Universidade do Vale do Taquari (Univates), students were invited to carry out a communit y inter vention with four social groups, using audiovisual language to address environmental issues. The aim of the study is to analyze the power of community journalism practices of making gestures of creation emerge. Here we ask how the interventions in the different social groups were dynamized, how the approaches given to environmental themes are characterized in audiovisuals and what approximations and distances are observed in relation to the ways of doing that have emerged. It is a qualitative, explorator y, descriptive and inter ventionist study. Fil: Mazzarino, Jane. Universidade do Vale do Taquari; Brasil. Fil: Zanatta, Lilian. Universidade do Vale do Taquari; Brasil.

Este artigo busca investigar a atual insurreição do movimento feminista no Brasil e a influência das redes sociais neste fenômeno. Para isso, relata a evolução do movimento através das quatro ondas, suas reivindicações e conquistas. A metodologia se apoia em estudos bibliográficos sobre movimentos sociais e sobre o feminismo, bem como no estudo de caso do coletivo feminista brasileiro Não Me Kahlo, e na análise qualitativa das publicações que tiveram como tema a violência, em sua página na rede social Facebook, durante os meses de janeiro e junho de 2019. Desta forma, o artigo considera que a quarta e atual fase do movimento feminista surge por meio de uma indignação feminina coletiva em razão dos crescentes casos de violência de gênero, e se impulsiona e populariza através das redes sociais.

O objetivo do artigo é investigar processos de comunicação e Educação Ambiental em resíduos sólidos domésticos no âmbito do Consórcio Público Intermunicipal para Assuntos Estratégicos do G8 (CIPAE G8), especialmente no que diz respeito às apropriações das metodologias de intervenção usadas na formação de multiplicadores. O estudo de caso foi realizado entre 2019 e 2020 e se utiliza de metodologia qualitativa, de base exploratória, descritiva e aplicada, apoiado em estudo bibliográfico, documental e de campo. O estudo, que se vincula diretamente ao Objetivo de Desenvolvimento Sustentável 11, demonstrou que o processo engendrou processos de comunicação ambiental comunitária, gerou diferentes formas de engajamento, foi beneficiado pelo contexto de proximidade e adequado às características dos multiplicadores e dos grupos de intervenção.

A presente pesquisa objetivou analisar artigos sobre a potência das hortas escolares para a formação de professores de Ciências da Natureza em Educação Ambiental, disponibilizados no Portal de Periódicos da CAPES, nos últimos 10 anos. Para tanto, foram utilizadas as palavras-chave “Educação Ambiental” E “formação de professores”; “educomunicação” E “formação de professores”; “hortas escolares” E “Educação Ambiental”; “hortas escolares”. Identificaram-se três grupos de estudos: a) Formação de Professores e Educação Ambiental; b) Educomunicação e Formação de Professores; c) Hortas escolares e Educação Ambiental. Os resultados apontaram que a escola é um espaço privilegiado para a Educação Ambiental, bem como para a Educação Alimentar e Nutricional, e que a horta é um laboratório vivo, com a educomunicação constituindo-se em um elemento fundamental para construir ecossistemas comunicativos.

To investigate the feasibility and preliminary effects of Pilates exercises in primigravida women.Single-blind randomized controlled feasibility trial.Community Pilates classes.Low-risk pregnant women.Pregnant women were randomly assigned to Pilates exercises (experimental) group for 6 consecutive weeks or usual antenatal care, the control group.The primary outcome was feasibility of Pilates classes. Secondary outcomes included quality of life, pain, and mobility.21 women were recruited to the trial. Eleven were randomly allocated to the experimental group and 10 to the control group. Retention of participants was excellent for the Pilates group (100%) compared to 70% in the control group. There were no adverse events. The Pilates group showed greater gains in quality of life on the SF-12 from the pre-test (M = 81.0, SD = 11.8) to the post-test (M = 83.3, SD = 8.52) compared to the control group (pre-test M = 69.78, SD = 15.9) (post-test M = 68.1, SD = 16.05) (Wald Chi-Square = 5.597, p = 0.018). Although the duration of labour was shorter in the Pilates group (Mdn = 215, IQR: 279 min) than usual care (Mdn = 458.5, IQR: 305 min), the difference was not statistically significant. There were no significant differences between groups for pain, mobility, abdominal separation, urinary continence, analgesia, or the mode of birth.Modified Pilates appears feasible and safe for low-risk pregnant women. Further research is needed in on this topic.

We characterized the world's second case with ascertained extreme resilience to autosomal dominant Alzheimer's disease (ADAD). Side-by-side comparisons of this male case and the previously reported female case with ADAD homozygote for the APOE3 Christchurch (APOECh) variant allowed us to discern common features. The male remained cognitively intact until 67 years of age despite carrying a PSEN1-E280A mutation. Like the APOECh carrier, he had extremely elevated amyloid plaque burden and limited entorhinal Tau tangle burden. He did not carry the APOECh variant but was heterozygous for a rare variant in RELN (H3447R, termed COLBOS after the Colombia-Boston biomarker research study), a ligand that like apolipoprotein E binds to the VLDLr and APOEr2 receptors. RELN-COLBOS is a gain-of-function variant showing stronger ability to activate its canonical protein target Dab1 and reduce human Tau phosphorylation in a knockin mouse. A genetic variant in a case protected from ADAD suggests a role for RELN signaling in resilience to dementia.

Purpose: Prostate cancer remains the second most frequent cause of tumor-related deaths in the Western world. Additional markers for the identification of prostate cancer development and progression are needed. Osteopontin (OPN), which activates matrix metalloproteinases (MMP), is considered a prognostic biomarker in several cancers. “In silico” and experimental approaches were used to determine whether OPN-mediated MMP activation may be a signal of prostate cancer progression.Experimental Design: Pearson correlation coefficients were computed for each OPN/MMP pair across seven publicly available prostate cancer gene expression data sets. Using Gene Set Enrichment Analysis, 101 cancer-related gene sets were analyzed for association with OPN and MMP-9 expression. OPN, MMP-9, MMP-2 tissue inhibitor of metalloproteinase-1 plasma levels, and MMP gelatinase activity were measured by ELISA and zymography in 96 and 92 patients with prostate cancer and benign prostatic hyperplasia, respectively, and 125 age-matched healthy men.Results: Computational analyses identified a significant correlation only between MMP-9 and OPN, and showed significant enrichment scores in “cell proliferation”, “genes constituting the phosphoinositide-3-kinase predictor”, “proliferation signature”, and “tumor metastasis” gene sets in association with both OPN and MMP-9. Plasma analyses revealed a significant increase in OPN and MMP-9 levels and activity in patients with prostate cancer in association with clinical variables (prostate-specific antigen >4 ng/mL and Gleason score >7). Significant correlation between OPN and MMP-9 levels were also observed. Mean plasma levels of OPN and MMP-9 decreased in patients with prostate cancer within 6 months after prostatectomy.Conclusions: The concordant computational and experimental data indicate that the extent of OPN pathway activation correlates with prostate cancer progression.

Supplementary Data from Activation of the Osteopontin/Matrix Metalloproteinase-9 Pathway Correlates with Prostate Cancer Progression

Numerous scientific evidences show that people with a mental disorder suffer not only from the disorder itself, but also, and perhaps above all, for the social and relational consequences that the disorder produces and which manifest themselves in the form of stigma. The improvement of mental health, with a view to Recovery, is in fact based on the possibility of taking advantage of socio-professional contexts in which users and operators have the opportunity to live meaningful relationships, and this can translate into a form of existence as much as possible rewarding and satisfying. The main purpose of the work was to investigate how much the "Innovative Technologies for Social Inclusion" project allowed to combine the promotion of the territory and the social inclusion of people with severe mental health problems. It was possible to analyze this element through the FPS Scale (personal and social functioning) and two evaluation grids administered. The New Communication and Information Technologies (ICT) have made it possible to rethink a new relationship between Digital Technology and the world of Disability; in fact they have allowed the patients of the Psychosocial Ria Community of Campolieto a more satisfactory quality of life and a better enhancement of the territory. These results offer promising preliminary evidence that the use of ICT provides an effective tool for promoting the social inclusion of discriminated groups of the population such as people with severe mental disorders.

Background: Amyloid-β (Aβ) is a principal cleavage product of amyloid-β protein precursor (AβPP) and is widely recognized as a key pathogenic player in Alzheimer’s disease (AD). Yet, there is increasing evidence of a neurotoxic role for the AβPP intracellular domain (AICD) which has been proposed to occur through its nuclear function. Intriguingly, there is a γ-secretase resident at the mitochondria which could produce AICD locally. Objective: We examined the potential of AICD to induce neuronal apoptosis when targeted specifically to the mitochondria and compared its mechanism of neurotoxicity to that of Aβ. Methods: We utilized transient transfection of HT22 neuronal cells with bicistronic plasmids coding for DsRed and either empty vector (Ires), Aβ, AICD59, or mitochondrial-targeted AICD (mitoAICD) in combination with various inhibitors of pathways involved in apoptosis. Results: AICD induced significant neuronal apoptosis only when targeted to the mitochondria. Apoptosis required functional mitochondria as neither Aβ nor mitoAICD induced significant toxicity in cells devoid of mitochondrial DNA. Both glutathione and a Bax inhibitor protected HT22 cells from either peptide. However, inhibition of the mitochondrial permeability transition pore only protected from Aβ, while pan-caspase inhibitors uniquely rescued cells from mitoAICD. Conclusion: Our results show that AICD displays a novel neurotoxic function when targeted to mitochondria. Moreover, mitoAICD induces apoptosis via a mechanism that is distinct from that of Aβ. These findings suggest that AICD produced locally at mitochondria via organelle-specific γ-secretase could act in a synergistic manner with Aβ to cause mitochondrial dysfunction and neuronal death in AD.

ResumoEste artigo busca contextualizar a criação da Universidade da Integração Internacional da Lusofonia Afro-Brasileira (UNILAB), seus objetivos e princípios com os principais aspectos relacionados à colaboração técnica internacional. A pesquisa realizada foi descritiva, qualitativa e bibliográfica. Após a coleta e análise dos dados, se buscou extrair as principais ideias apresentadas, de modo a alcançar os objetivos propostos neste trabalho. Ao final da pesquisa, foi possível perceber que a UNILAB possui como objetivo formar pessoas para contribuir com os países membros da Comunidade dos Países de Língua Portuguesa (CPLP), especialmente as nações africanas. Os cursos da UNILAB são ministrados principalmente em áreas (formação de professores, desenvolvimento agrário, gestão, saúde pública, dentre outros) de interesse mútuo entre os países participantes. A cooperação técnica internacional é um importante elemento para a promoção do desenvolvimento social, econômico e cultural, bem como no compartilhamento de conhecimentos, experiências e boas-práticas. Neste contexto, a UNILAB possui, desde sua criação, um caráter voltado para a cooperação internacional solidária e compromissada com a interculturalidade. Palavras-chave: Colaboração Técnica. Internacionalização do Ensino. Interculturalidade. AbstractThis article seeks to contextualize the creation of the University of International Integration of Afro-Brazilian Lusophony (UNILAB), its objectives and principles with the main aspects related to international technical collaboration. The research carried out was descriptive, qualitative and bibliographic. After collecting and analyzing the data, we sought to extract the main ideas presented, in order to achieve the objectives proposed in this work. At the end of the research, it was possible to see that UNILAB aims to train people to contribute to the member countries of the Community of Portuguese Speaking Countries, especially African nations. UNILAB courses are taught mainly in areas (teacher training, agricultural development, management, public health, among others) of mutual interest between the participating countries. International technical cooperation is an important element in promoting social, economic and cultural development, as well as in the sharing of knowledge, experiences and good practices. In this context, UNILAB has, since its creation, a character focused on international solidarity cooperation and committed to interculturality. Keywords: Technical Collaboration. Internationalization of Teaching. Interculturality.

Uma escola pública, uma área verde, um grupo de alunos de 11 e 12 anos e um grupo de pesquisa que tinha como objetivo experienciar possibilidades de sensibilização ambiental baseadas em um método que explora atividades ao ar livre, visando à produção de uma subjetividade ambiental. Estes elementos foram geradores de encontros semanais que duraram três meses, optando-se por avaliar um processo de educação ambiental em continuidade, a fim de se estabelecer um vínculo com o grupo e observar com profundidade processos que os pesquisadores só investigavam em encontros esporádicos. A estratégia do grupo de pesquisa foi baseada no método do Aprendizado Sequencial de Joseph Cornell e na proposta ecosófica de Felix Guattari e suas três ecologias: subjetiva, social e ambiental. O objetivo foi investigar possibilidades de produção de subjetividade ambiental a partir da proposta ecosófica e de um método de educação ambiental ao ar livre. Como elemento conclusivo fundamental consideramos a evidência de que o contato coletivo com a natureza afeta as relações sociais, favorecendo a percepção do outro na sua singularidade. Ou seja, a natureza atua como facilitadora da abertura para o outro. A ecologia social e subjetiva ou mental, no caso em estudo, foram favorecidas pela ecologia do ambiente.

Alzheimer’s disease (AD) is the most common cause of dementia among older adults.APOE3Christchurch (R136S,APOE3Ch) variant homozygosity was reported in an individual with extreme resistance to autosomal dominant AD due to thePSEN1E280A mutation. This subject had a delayed clinical age at onset and resistance to tauopathy and neurodegeneration despite extremely high amyloid plaque burden. We established induced pluripotent stem (iPS) cell-derived cerebral organoids from this resistant case and from a non-protected kindred control (withPSEN1E280A andAPOE3/3). We used CRISPR/Cas9 gene editing to successfully remove theAPOE3Chto wild type in iPS cells from the protected case and to introduce theAPOE3Chas homozygote in iPS cells from the non-protected case to examine causality. We found significant reduction of tau phosphorylation (pTau 202/205 and pTau396) in cerebral organoids with theAPOE3Chvariant, consistent with the strikingly reduced tau pathology found in the resistant case. We identified Cadherin and Wnt pathways as signaling mechanisms regulated by theAPOE3Chvariant through single cell RNA sequencing in cerebral organoids. We also identified elevated β-catenin protein, a regulator of tau phosphorylation, as a candidate mediator ofAPOE3Chresistance to tauopathy. Our findings show thatAPOE3Chis necessary and sufficient to confer resistance to tauopathy in an experimentalex-vivomodel establishing a foundation for the development of novel, protected case-inspired therapeutics for tauopathies, including Alzheimer’s.

A typical characteristic of the wine supply chain in the Old World is the significant share of cooperatives in wine-making that coexists with investor-owned firms and on-farm wine-makers. This paper analyzes the determinants of whether grape growers deliver their grapes to a cooperative winery of which they are members, sell their grapes to outside wineries, or make their own wine on their farm. Our analysis is based on data from a typical wine-producing area in Northern Italy. The explanatory variables comprise the potential prices linked to the different grape allocations and various farmer and farm characteristics. The high share of farmers delivering their grapes to cooperatives can, to a large extent, be explained by their higher price relative to one of the spot markets. On-farm wine-making is favored by larger farms and more educated farmers.

O presente artigo busca analisar como o uso das redes sociais contribui para o desenvolvimento da Quarta Onda Feminista no Brasil. Dados como o aumento do número de pesquisas em plataformas de busca online e a multiplicação de páginas de conteúdo feminista em redes sociais destacam o crescente debate sobre o assunto, e justificam a relevância do estudo. O artigo relata o desenvolvimento do movimento feminista, do seu surgimento até a atual insurreição, e a maneira como este se formou. Para isso, utiliza como metodologia estudos bibliográficos sobre feminismo e sobre o uso do espaço virtual por parte de movimentos sociais, bem como o estudo de caso do coletivo feminista brasileiro Não Me Kahlo. Também foi realizada a entrevista com uma das suas fundadoras e a análise quantitativa das publicações na página do coletivo na rede social Facebook, durante os meses de janeiro e junho de 2019. A partir desta metodologia, o estudo identifica a associação direta entre a atual insurreição feminista e o uso das redes sociais que são utilizadas como ferramentas de comunicação, organização epopularização do conteúdo feminista. A pesquisa também aponta os benefícios e as dificuldades que o uso das redes sociais acrescenta ao atual cenário da militância feminista.

Mexedrone is a synthetic cathinone structurally related to mephedrone, which belongs to the class of N-alkyl cathinone derivatives, whose metabolic profile has not been fully clarified yet. This study considers the in vitro phase I metabolism of mexedrone, to pre-select the most appropriate marker(s) of intake. Mexedrone was incubated in the presence of either human liver microsomes or single recombinant CYP450 isoforms. The metabolic profile was outlined by ultra-high-performance liquid chromatography coupled to both high- and low-resolution mass spectrometry. In detail, the phase I metabolic profile of mexedrone was initially defined by a time-of-flight analyzer, while the chemical structures of the detected metabolites and the potential presence of minor metabolites were subsequently studied by tandem mass spectrometry, using a triple quadrupole analyzer. The main phase I metabolic reactions were hydroxylation and N- and O-dealkylation. The CYP450 isoforms most involved were CYP2C19, responsible for the formation of both hydroxylated and dealkylated metabolites, followed by CYP2D6 and CYP1A2, involved in the hydroxylation reactions only. Finally, a significant fraction of mexedrone unchanged was also detected. Based on this evidence, the most appropriate markers of intake are mexedrone unchanged and the hydroxylated metabolites.

The transition to a low-carbon society will completely change the structure of energy systems from a standalone hierarchical centralised vision to cooperative and distributed Multi-Energy Systems. The analysis of these complex systems requires the collaboration of researchers from different disciplines in the energy, ICT, social, economic, and political sectors. Combining such disparate disciplines into a single tool for modeling and analyzing such a complex environment as a Multi-Energy System requires tremendous effort. Researchers have overcome this effort by using co-simulation techniques that give the possibility of integrating existing domain-specific simulators in a single environment. Co-simulation frameworks, such as Mosaik and HELICS, have been developed to ease such integration. In this context, an additional challenge is the different temporal and spatial scales that are involved in the real world and that must be addressed during co-simulation. In particular, the huge number of heterogeneous actors populating the system makes it difficult to represent the system as a whole. In this paper, we propose a comparison of the scalability performance of two major co-simulation frameworks (i.e. HELICS and Mosaik) and a particular implementation of a well-known multi-agent systems library (i.e. AIOMAS). After describing a generic co-simulation framework infrastructure and its related challenges in managing a distributed co-simulation environment, the three selected frameworks are introduced and compared with each other to highlight their principal structure. Then, the scalability problem of co-simulation frameworks is introduced presenting four benchmark configurations to test their ability to scale in terms of a number of running instances. To carry out this comparison, a simplified multi-model energy scenario was used as a common testing environment. This work helps to understand which of the three frameworks and four configurations to select depending on the scenario to analyse. Experimental results show that a Multi-processing configuration of HELICS reaches the best performance in terms of KPIs defined to assess the scalability among the co-simulation frameworks.

Atualmente verifica-se uma ligação cada vez mais humanizada entre os animais de companhia e o ser humano, e isso reflete na alimentação fornecida aos animais. Há uma tendência por parte dos tutores em querer oferecer uma dieta mais próxima da sua alimentação (alimentação cozida) ou fornecer uma dieta mais próxima da ancestralidade canina (alimentação crua). Sendo assim, o objetivo da presente revisão bibliográfica é apresentar os pontos positivos e negativos da alimentação fornecida crua ou cozida para cães e gatos. Foi realizada uma busca de artigos científicos publicados de 2009 até 2022. As pesquisas foram realizadas nas seguintes plataformas: CAPES, PUBMED e GOOGLE ACADÊMICO. Os artigos relacionados à dieta crua demonstraram a existência de componentes com potencial zoonótico que pode afetar tanto o animal quanto o tutor. Já em relação à dieta cozida não se verificou a presença de patógenos na dieta, no entanto, muitas destas não atendem as exigências nutricionais dos animais. Sendo assim, destaca-se o papel do tutor em buscar informações junto aos profissionais da área de nutrição para que a dieta não ofereça riscos ao animal e nem ao tutor, além disso, que atenda as exigências nutricionais. Currently, there is an increasingly humanized connection between companion animals, and this is reflected in the food provided to the animals. There is a tendency on the part of tutors to want to offer a diet closer to their food (cooked food) or to provide a diet closer to canine ancestry (raw food). Therefore, the objective of this literature review is to present the positive and negative points of food provided raw or cooked for dogs and cats. A search of scientific publications published from 2009 to 2022 was carried out. How the following platforms were carried out: CAPES, PUBMED and ACADEMIC GOOGLE. Diet-related zoonotic articles can exist with both the animal and the tutor potential. In relation to the cooked diet, they do not identify with the presence of animals, often do not meet the nutritional requirements. Thus, to seek information from the tutor to professionals so that the diet together are not risks of animal nutrition and neither to the tutor, be it of that, as nutritional requirements. Actualmente, existe una conexión cada vez más humanizada entre los animales de compañía y esto se refleja en la alimentación que se les proporciona a los animales. Hay una tendencia por parte de los tutores a querer ofrecer una alimentación más cercana a su alimentación (comida cocinada) o a proporcionar una alimentación más cercana a la ascendencia canina (comida cruda). Por lo tanto, el objetivo de esta revisión bibliográfica es presentar los puntos positivos y negativos de los alimentos proporcionados crudos o cocinados para perros y gatos. Se realizó una búsqueda de publicaciones científicas publicadas desde 2009 hasta 2022. Cómo se realizaron las siguientes plataformas: CAPES, PUBMED y GOOGLE ACADÉMICO. Los artículos zoonóticos relacionados con la dieta pueden existir tanto con el animal como con el potencial tutor. En relación con la dieta cocinada, no se identifican con la presencia de animales, muchas veces no cumplen con los requerimientos nutricionales. Así, buscar información del tutor a los profesionales para que la dieta en conjunto no sean riesgos de la nutrición animal y tampoco al tutor, sea de eso, como requerimientos nutricionales.

Este artigo tem como objetivo descrever[A1] duas experiências de vivências com a natureza: um no município do Crato, estado do Ceará, Região Nordeste do Brasil, outro em Coimbra, na província da Beira Litoral, Região do Centro, Portugal. Os projetos têm suas singularidades, mas também convergências, o que possibilita a realização do estudo. Observaram-se as crianças participantes, registrando-se as ocorrências e analisando-se o que sucede em decorrência das atividades. As análises são compostas por três dimensões: ambientes, mediadores e métodos. Os dados empíricos apontam que, mesmo que de forma diferenciada, ambas as experiências favorecem o desenvolvimento infantil em seus diversos aspectos, conforme o foco dos métodos. Enquanto nas vivências com a natureza a interação com o contexto natural esteve focada em gerar experiências que exploravam os sentidos, no Limites Invisíveis buscava-se provocar experiências de aprendizagem. Em ambas as propostas o ambiente natural parece despertar nas crianças o que está latente, que não emerge, muitas vezes, por falta deste contato primordial com ambientes naturais.

O artigo problematiza o panorama da formação de professores em Educação Ambiental (EA) entre desafios e potências. Tem-se por objetivo mapear e analisar a produção acadêmica e científica entre 1992 e 2017 em língua espanhola e em língua portuguesa disponível no Portal de Periódicos da Capes referente à formação de professores em EA. A metodologia utilizada foi quanti-qualitativa, bibliográfica e documental a partir da Análise Integrativa. Os resultados demonstram desafios infraestruturais, pessoais, institucionais e ideológicos que atravancam o desenvolvimento de formações significativas e potências quando se utiliza diferentes espaços, agentes e instituições para gerar experimentações através de metodologias plurais e diversificadas no fazer EA através da docência.

Cochliomyia hominivorax is the dipteran that causes the worst and most serious types of myiasis in animals and humans in the Americas. They are parasitic affections caused by the massive infestation of larvae of this species on the tissues of living mammals. The routine treatment uses chemosynthetic products that have high toxicity and can cause several injuries to animal and human health, besides environmental contamination. The development of more friendly alternatives to the treatment of this disease is an important knowledge gap nowadays. Citronella (Cymbopogon winterianus) is the most widely cultivated medicinal plant in tropical and subtropical regions. Essential oils have a recognised repellent and larvicidal activity against a wide variety of insects. This work aimed to evaluate the repellent, ovicidal and larvicidal efficacy of a topical commercial formulation composed of 15% (w/v) nanoemulsified Citronella essencial oil (Nano Citronela Plus®) in in vitro trials on specimens of C. hominivorax created under laboratory conditions. Nano Citronela Plus® is an oil-in-water nanoemulsion obtained by high-pressure homogenization method. The composition of tested nanoemulsion was Cymbopogon winterianus essential oil, vanillin, caprylic/capric triglyceride, polysorbate 80, sorbitan oleate, tocopheryl acetate, sodium benzoate and water. Nano Citronela Plus® demonstrated 100% lethality on eggs and first and second stage larvae of C. hominivorax. Mortality in the larvae of third stage was 69.33% and inhibited the oviposition of C. hominivorax females. Nano Citronela Plus® proved to be a possible herbal alternative for the control and treatment of myiasis caused by C. hominivorax. In vivo studies may test this hypothesis.

Recently, automation is gaining an even more important role in the port and maritime industry. In particular, several technological innovations are changing both the freight and passenger transport sector. The introduction of these technologies in port terminals (smart ports) require involved stakeholders to adapt their asset and organisations in order to improve the economic competi-tiveness in global markets. The geographical context where new technologies are put in place can also influence their deployment and foreseen impacts. Hence, in order to take the proper decisions at a strategic level and maximize the positive effects in a selected scenario, a feasibility analysis is essential. In the present study, this challenge is addressed for the Adriatic region by proposing a proce-dure for evaluating and selecting the most promising innovations. Several rele-vant stakeholders from the selected area are inquired to assess the relevance and deployment difficulties for a set of new technologies dealing with automation in port areas. Then, the impacts on technical operation and labour market are as-sessed, thus, providing valuable information to support the regional organisations in facing the change and deploying procedures to be potentially replicated in other geographical areas.

Big data technologies offer new opportunities for the port and maritime industry in both freight and passengers transport. In order to ensure the success of a new big data project in a specific environment, it is essential to assess the benefits and the efforts required by its implementation. The present work addresses this topic for a specific area: the Adriatic region. Several relevant stakeholders from the selected area have been inquired to assess the relevance and deployment difficulties for a set of promising big data technologies. The study provides a picture of the current state and the future outlook for the Adriatic region, being useful for local operators and a valuable example to be replicated in other contexts.

Environmental sustainability and energy efficiency are some of the most challenging objectives to be pursued in port areas. In this context, the SUSPORT project aims to provide its contribution, affecting the Adriatic area. In the initial phase, before applying new technologies/solutions to enhance port sustainability, the baseline status shall be assessed in order to evaluate the impact of tested measures. To this end, a review of the peculiarities of the main ports of the Adriatic Sea (Italian and Croatian), including the evaluation of their carbon footprint should be carried out. The present work reports the results of this phase, focusing on the main statistics of the involved ports and their greenhouse gases inventory at an aggregated level.

This article reports the results obtained from the investigation of the influence of miconazole administration on the physiological fluctuation of the markers of the steroid profile included in the “steroidal module” of the Athlete Biological Passport. Urines collected from male Caucasian subjects before, during, and after either systemic (i.e., oral and buccal) or topical (i.e., dermal) treatment with miconazole were analyzed according to validated procedures based on gas chromatography coupled to tandem mass spectrometry (GC–MS/MS) (to determine the markers of the steroid profile) or liquid chromatography coupled to MS/MS (LC–MS/MS) (to determine miconazole urinary levels). The results indicate that only after systemic administration, the markers of the steroid profile were significantly altered. After oral and buccal administration, we have registered (i) a significant increase of the 5α‐androstane‐3α,17β‐diol/5β‐androstane‐3α,17β‐diol ratio and (ii) a significant decrease of the concentration of androsterone, etiocholanolone, 5β‐androstane‐3α,17β‐diol, and 5α‐androstane‐3α,17β‐diol and of the androsterone/etiocholanolone, androsterone/testosterone, and 5α‐androstane‐3α,17β‐diol/epitestosterone ratios. Limited effects were instead measured after dermal intake. Indeed, the levels of miconazole after systemic administration were in the range of 0.1–12.5 μg/ml, whereas after dermal administration were below the limit of quantification (50 ng/ml). Significant alteration started to be registered at concentrations of miconazole higher than 0.5 μg/ml. These findings were primarily explained by the ability of miconazole in altering the kinetic/efficacy of deglucuronidation of the endogenous steroids by the enzyme β‐glucuronidase during the sample preparation process. The increase of both incubation time and amount of β‐glucuronidase was demonstrated to be effective countermeasures in the presence of miconazole to reduce the risk of uncorrected interpretation of the results., The results obtained from the investigation of the influence of miconazole administration on the parameters included in the “steroidal module” of the Athlete Biological Passport were presented. After oral and buccal administration of miconazole, the levels of the markers of the steroid profile were significantly altered, whereas no effects were registered after topical administration. These findings were primarily explained by the effect of miconazole on the kinetics/efficacy of the enzymatic hydrolysis during the sample preparation process.

Esse estudo analisou a relevância da experiência docente na formação inicial de professores de Educação Física para atuar com o tema da Educação Ambiental. A pesquisa caracterizou-se como qualitativa. Ela foi desenvolvida tendo como sujeitos três acadêmicos de Educação Física – Licenciatura. Eles participaram de uma formação ambiental por meio de vivências com a natureza ministrada por um professor/pesquisador universitário. A partir dessa experiência, os acadêmicos planejaram aulas com a temática Educação Ambiental e as ministraram para estudantes no Ensino Fundamental. Após a experiência docente, os três acadêmicos foram entrevistados. Constatou-se que experimentar vivências com a natureza foi fundamental para os acadêmicos vislumbrarem possibilidades de articulação da Educação Física com a Educação Ambiental. A necessidade de “se colocar no lugar de professor” é percebida no decorrer da pesquisa como fundamental no processo de “constituir-se” professor. No exercício cotidiano da docência o professor constrói a sua identidade docente, ressignifica saberes e torna-se autor do seu fazer pedagógico.

3-(1-Naphthalenylmethyl)-1-pentyl-1H-indole (JWH-175) is a synthetic cannabinoid illegally marketed for its psychoactive cannabis-like effects. This study aimed to investigate and compare in vitro and in vivo pharmacodynamic activity of JWH-175 with that of 1-naphthalenyl (1-pentyl-1H-indol-3-yl)-methanone (JWH-018), as well as evaluate the in vitro (human liver microsomes) and in vivo (urine and plasma of CD-1 male mice) metabolic profile of JWH-175. In vitro binding studies showed that JWH-175 is a cannabinoid receptor agonist less potent than JWH-018 on mouse and human CB1 and CB2 receptors. In agreement with in vitro data, JWH-175 reduced the fESPS in brain hippocampal slices of mice less effectively than JWH-018. Similarly, in vivo behavioral studies showed that JWH-175 impaired sensorimotor responses, reduced breath rate and motor activity, and increased pain threshold to mechanical stimuli less potently than JWH-018. Metabolic studies demonstrated that JWH-175 is rapidly bioactivated to JWH-018 in mice blood, suggesting that in vivo effects of JWH-175 are also due to JWH-018 formation. The pharmaco-toxicological profile of JWH-175 was characterized for the first time, proving its in vivo bio-activation to the more potent agonist JWH-018. Thus, it highlighted the great importance of investigating the in vivo metabolism of synthetic cannabinoids for both clinical toxicology and forensic purposes.

Fil: Leconte, María Corina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Mazzarino, María Julia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. El compostaje es un proceso por el que se logra reutilizar y valorizar residuos de origen orgánico, obteniendo materia orgánica estable y madura, libre de patógenos y semillas de malezas, que aplicada al suelo mejora sus propiedades físicas, químicas y biológicas. Existe mucha información sobre este proceso, pero algunas son erróneas o no del todo correctas y se han transformado en mitos. Intentaremos esclarecer algunos de ellos.

Carvacrol has shown antimicrobial activity against a wide range of Gram-positive and Gram-negative bacteria. However, its hydrophobic nature, volatility, and susceptibility to oxidation mediated by light or heat limit its use. Nanoencapsulation provides a protective barrier against degradation and volatilization processes and improves the dispersibility of bioactive compounds. A simple, rapid, and sensitive analytical method was developed and validated by UV spectrophotometry to quantify carvacrol in a nanoemulsion obtained by self-nanoemulsification, using acetonitrile as a solvent to recover the analyte from the nanoemulsion. The UV scanning spectrum of carvacrol (240–300 nm) in acetonitrile showed maximum absorbance at wavelength 275 nm, typical of this monoterpene. The calibration curve was linear from 15 to 65 μg/ml, with a determination coefficient of 0.999. The detection and quantification limits found were 1.6455 and 4.9863 μg/ml, respectively. The validation results confirmed that the method developed is specific, linear, accurate, and precise and it can be successfully applied for the determination of encapsulation efficiency and the content of carvacrol present in a nanoemulsion.

In this work, we address the beneficial role of noise in two different contexts, the human brain and financial markets. In particular, the similitude between the ability of financial markets to maintain in equilibrium asset prices is compared with the ability of the human nervous system to balance a stick on a fingertip. Numerical simulations of the human stick balancing phenomenon show that after the introduction of a small quantity of noise and a proper calibration of the main control parameters, intermittent changes in the angular velocity of the stick are able to reproduce the most basilar stylized facts involving price returns in financial markets. These results could also shed light on the relevance of the idea of the “planetary nervous system”, already introduced elsewhere, in the financial context.

Photobiomodulation (PBM) is rapidly gaining traction as a valuable tool in dermatology for treating many inflammatory skin conditions using low levels of visible light or near-infrared radiation. However, the physiological regulatory pathways responsible for the anti-inflammatory effect of PBM have not been well defined. Since previous studies showed that nuclear factor-erythroid 2 like 2 (Nrf2) is a master regulator of the skin inflammatory response, we have addressed its role in controlling inflammation by PBM. Primary human keratinocytes (KCs) stimulated with 2,4-dinitrochlorobenzene (DNCB) to mimic pro-inflammatory stress were illuminated with two wavelengths: 660 nm or 520 nm. Both lights significantly reduced the mRNA expression of the DNCB-triggered TNF-α, IL-6, and IL-8 cytokines in KCs, while they enhanced Nrf2 pathway activation. PBM-induced Nrf2 is a key regulator of the inflammatory response in KCs since its absence abolished the regulatory effect of light on cytokines production. Further investigations of the mechanisms contributing to the immunoregulatory effect of PBM in inflamed human skin explants showed that 660 nm light prevented Langerhans cells migration into the dermis, preserving their dendricity, and decreased pro-inflammatory cytokine production compared to the DNCB-treated group. This study is the first to report that the PBM-mediated anti-inflammatory response in KCs is Nrf2-dependent and further support the role of PBM in skin immunomodulation. Therefore, PBM should be considered a promising alternative or complementary therapeutic approach for treating skin-related inflammatory diseases.

Background Pilates has growing appeal to pregnant women, as a form of exercise and relaxation. It is purported to benefit lumbo-pelvic stability, as well as motor control, strength and endurance. Some suggest that modified Pilates exercises may assist low risk pregnant women to enjoy a healthier pregnancy and prepare for the physical demands of labour and birth. The feasibility and safety of Pilates during pregnancy is poorly understood. We describe the protocol for a feasibility study designed to compare a midwife-led 6-week community-based Pilates intervention with standard antenatal care. Methods A convenience sample of 30 low-risk pregnant women will be recruited from private obstetric clinics in Melbourne, Australia. Participants shall be randomly allocated to a six-week, 1-h weekly Pilates exercises group session or to usual care. The Pilates exercise class will have a warm-up phase, Pilates exercises, breathing exercises, and a cool down phase. Exercises have been designed to prepare for active birth. The primary outcome will be feasibility of implementation, determined by recruitment, retention, adherence and safety. Secondary outcomes include women's health (quality of life, pain, mobility for daily activities, lower extremity performance, abdominal separation, continence) and labour and birth outcomes (duration of first stage and second stage labour, analgesia used, mode of birth). Validated questionnaires will include the Quality of life 12-item short form survey; Pregnancy Mobility Index, and International Consultation on Incontinence Questionnaire. Lower extremity performance and abdominal separation will also be measured. Discussion This trial will provide preliminary data regarding the feasibility and safety of Pilates exercise in healthy pregnant women. It will also provide preliminary outcome data used to inform the design of a future large scale, multi-centre RCT. Trial registration This clinical trial has been registered with the Australian and New Zealand Clinical Trials Registry 2016 (ACTRN12616000809437).

O objetivo é investigar um processo de formação continuada em Educação Ambiental vivencial com professores de Educação Infantil, no qual utilizou-se o Método do Aprendizado Sequencial como forma de explorar as possibilidades dos pátios das escolas. Partindo de uma abordagem qualitativa, de caráter descritivo, adotou-se a perspectiva intervencionista (pesquisa-ação), baseada nas pesquisas bibliográfica, documental e de campo. Fez-se uso da observação, registros (em diários de bordo e fotografias) e entrevistas individuais e em grupo. Os resultados referem-se a dois momentos do processo: o planejamento e a primeira etapa da formação continuada e as implicações desta na prática das professoras que participaram da segunda etapa do programa.

We present a quick and simple multi-targeted analytical workflow based on ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry for the screening in dried blood spots and dried plasma spots of a wide variety of drugs with different chemical properties. Seven different microsampling devices were evaluated in view of their application for the detection of the selected target analytes in the framework of doping control analysis. The extraction of the analytes was optimized by assessing the efficacy of protocols based on ultrasonication with aqueous buffers and/or organic solvents of different polarities. Optimal recoveries were obtained by using pure methanol or mixtures of methanol/acetonitrile and methanol/isopropanol, depending on both the device and the target analytes. The method was fully validated according to both ISO17025 and the requirements of the World Anti-Doping Agency: all the analytes were clearly distinguishable from the matrix, with limits of detection in the range of 0.1-3.0 ng mL

O objetivo é investigar a potência de intervenções em educação ambiental provocadoras de experiências ecosóficas para ampliar o envolvimento de mulheres rurais com suas paisagens. Trata-se de uma pesquisa de cunho intervencionista, apoiada em estudo bibliográfico, documental e de campo. O estudo delimita-se a um grupo de nove mulheres rurais do município de Lagoão, Rio Grande do Sul. As análises foram organizadas em duas categorias que emergiram ao longo do estudo: a) a proposta de intervenção, suas intenções e potências; b) ressignificações da paisagem de vida. Como resultados, identificou-se que as metodologias participativas e as vivências na natureza promoveram processos sensibilizadores capazes de provocar transformações ecológicas na vida das mulheres rurais; e que as paisagens socioambientais, com as intervenções, ficaram mais claras e assumiram algumas novas nuances.

The aim of the paper presented herein is the description and safety evaluation of the process of dissolution of an 86-microneedle patch composed of hyaluronic acid, when applied topically to human abdominal skin explants. Such explants were chosen to replace the inability of obtaining periorbital skin. In order to evaluate penetration and dissolution of the microneedles, we employed histochemical methods and a fluorescent dye FITC (fluorescein isothiocyanate).Abdominoplasty human skin explants were treated with square microneedle patches with a 1.5-cmThe application of the MN patch to excised skin explants showed these microneedles to be non-invasive into the dermis of the skin. Skin puncturing with MN patches revealed 17 different sites of microneedle penetration immediately afterwards and 4 sites, 2 h later. Although there were some variances in the epidermal depth of penetration, these variances did not impact on cell viability. The hyaluronic acid-based microneedles having 450 µm in length penetrated the epidermis at an averaged depth by 26 µm without disrupting skin cell viability and without causing an inflammatory response. Hyaluronic acid could be detected in most of these penetration sites, with no diffusion into the dermis, which is important for cosmetic applications. FITC analysis uncovered fluorescein isothiocyanate distribution within microneedle insertion site, which remained steady after 2 and 6 h of experimentation.Using ex vivo tracer staining studies, we have shown that the evaluated microneedle applicator is capable of penetrating the skin epidermis and delivering substances embedded in the needle polymer matrix. In addition, the tested product was shown to be safe, which provides a broad perspective for delivering cosmetic and pharmaceutic agents.Le but de cet article est de décrire et évaluer l’innocuité du processus de dissolution d'un patch de 86 micro-aiguilles composé d'acide hyaluronique, lorsqu'il est appliqué par voie topique sur des explants de peau abdominale humaine. De tels explants ont été choisis pour palier à l'impossibilité d'obtenir une peau périorbitaire. Afin d'évaluer la pénétration et la dissolution des micro-aiguilles, nous avons utilisé des méthodes histochimiques et un marqueur fluorescent FITC (isothiocyanate de fluorescéine). MÉTHODES: Les explants de la peau humaine de l'abdominoplastie ont été traités avec des patchs à microaiguilles, carrés d'une surface de 1,5 cm2, contenant 86 micro-aiguilles et ayant 450 ± 23,5 μm de hauteur avec 1 mm d'espacement entre les micro-aiguilles. Un traitement histologique et des colorations ont été réalisée pour observer la viabilité cellulaire et les glycosaminoglycanes. La diffusion de FITC a été observée en épifluorescence. Le pourcentage de surface colorée pour chaque traitement a été comparé à des échantillons témoins non traités à différents temps de cinétique. Un test de Mann-Whitney a été utilisé pour identifier la différence entre deux populations (sites de pénétration des micro-aiguilles et peau normale) avec une limite de significativité statistique de P0.05. RÉSULTATS: L'application du patch MN sur les explants de peau a montré que ces micro-aiguilles ne Pénétraient pas dans le derme de la peau. L’application cutanée des patchs MN a révélé 17 sites différents de pénétration de micro-aiguille immédiatement après l’application et 4 sites, 2 heures plus tard. Bien qu'il y ait eu quelques variations dans la profondeur de pénétration épidermique, ces variations n'ont pas eu d'impact sur la viabilité cellulaire. Les micro-aiguilles à base d'acide hyaluronique d'une longueur de 450 μm ont pénétré l'épiderme à une profondeur moyenne de 26 μm sans perturber la viabilité des cellules de la peau et sans provoquer de réponse inflammatoire. L'acide hyaluronique composant les micro-aiguilles a été détecté sur la plupart de ces sites de pénétration, sans diffusion dans le derme, ce qui est important pour les applications cosmétiques. L'analyse FTIC a révélé une distribution d'isothiocyanate de fluorescéine dans le site d'insertion de micro-aiguille qui est restée stable après 2 et 6 heures d'expérimentation.En utilisant des études de coloration de traceurs sur explant de peau humaine ex vivo, nous avons montré que l'applicateur de micro-aiguille est capable de pénétrer l'épiderme et de délivrer des substances incorporées dans la matrice polymère de l'aiguille. De plus, le produit testé s'est avéré sûr et bien toléré, ce qui ouvre une large perspective pour l'administration d'agents cosmétiques et pharmaceutique.

Twenty-two pharmaceutical formulations containing prednisolone or prednisone commercially available in Italy, Belgium, Spain, Brazil, and India were analyzed through a specific gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) method. All of them showed typical non-endogenous δ13 C values, except for the Belgian nasal spray, Sofrasolone®, with a less depleted 13 C content (-17.84 ± 0.18‰). Observational studies were performed on two volunteers in therapy with Sofrasolone® to confirm the applicability of the method and to suggest adequate interpretation criteria also in the case of drugs with less negative δ13 C values. Urine samples were collected before, during, and within the 36 hours after the administration of the spray. Both δ13 C values and urinary concentrations of prednisolone and prednisone were evaluated. All samples were subjected to an adequate pre-treatment (enzymatic hydrolysis, liquid/liquid extraction, and two sequential HPLC steps) before injection to the GC-C-IRMS instrument, according to the method recently developed and validated in our laboratory. Pregnanediol (PD), tetrahydro-11-deoxycortisol (THS), and pregnanetriol (PT) were selected as endogenous reference compounds (ERC). The excretion profile was estimated through liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method used routinely for the quali-quantitative detection of glucocorticoids. δ13 C values and urinary levels of prednisolone and prednisone were also determined after the intake of one single vial of Sintredius®, a prednisolone oral formulation with a conventional more negative δ13 C value (-29.28 ± 0.25‰). Finally, the potential masking effect that combined therapy with Sofrasolone® and Sintredius® could induce on the IRMS findings was investigated.

This article presents newly developed screening and confirmation analytical procedures to detect the misuse of nine prolyl-hydroxylase inhibitors of the hypoxia-inducible factor: daprodustat, desidustat, FG2216, IOX2, IOX4, JNJ-42041935, molidustat, roxadustat and vadadustat, targeting either the parent drugs and/or their main metabolite(s). For the sample pretreatment, different extraction protocols and technologies were evaluated. The instrumental analysis was performed by ultra-high-performance liquid chromatography coupled to either high- or low-resolution mass spectrometry. The chromatographic separation was performed on a C18 column, employing water and acetonitrile, both containing 0.1% formic acid, as mobile phase. Detection was achieved using as analyzer either a triple quadrupole or an Orbitrap, with positive and negative electrospray ionization and different acquisition modes. Validation of the procedures was performed according to the ISO 17025 and World Anti-Doping Agency guidelines. The methods do not show any significant interference at the retention times of the analytes of interest. The extraction efficiency was estimated to be greater than 75% for all analytes and the matrix effect smaller than 35%. Detection capability was determined in the range of 0.25–2.0 for the screening procedure and in the range of 0.5–2.0 ng/mL for the confirmation procedure, that is, in a range of concentration small enough to reveal the abuse of the compounds considered, in case they are used as performance-enhancing agents. The repeatability of the relative retention times (CV%

A internet possibilita às organizações sociais o papel de protagonistas na consecução do direito à informação, de modo a estimular a participação cidadã no debate público sobre as mais diversas questões, dentre as quais a hídrica. Este estudo analisa notícias veiculadas por duas organizações governamentais responsáveis pela gestão das águas no contexto brasileiro: uma nacional e outra do estado de São Paulo. Discute-se se suas práticas comunicativas atendem aos pressupostos legais do direito à informação no processo de agendamento e enquadramento da crise hídrica de São Paulo. O estudo é descritivo e analítico, qualitativo, de base bibliográfica e documental. A amostra é não probabilística, por tipicidade e acessibilidade. Emergiram quatro categorias de análise a partir de uma diversidade de elementos que compuseram a macrocategoria enquadramento: fontes, conflitos, responsabilidade e soluções. Concluiu-se que a organização estadual enquadra a crise como uma situação “sob controle”, enquanto a organização nacional busca explicar tecnicamente os mecanismos para o seu enfrentamento. De modo geral, e considerando-se a relevância do problema, as duas organizações publicaram pouco e informaram predominantemente a partir de interesses institucionais e não do interesse público. Por fim, verifica-se que o papel das organizações de caráter ambiental deve ser ampliado em se tratando da implementação do direito à informação ambiental.

In Brazil, the National Policy on Solid Waste (Law No. 12,305) brought new guidelines for waste management and incorporated into its text principles that were previously only in the doctrine, especially the one that deals with the systemic view, which considers the variables environmental, social, economic, cultural, technological and public health. This article analyzes the advantages and challenges of inter-municipal consortium for the integrated management of domestic solid waste, in the light of the principle of systemic vision, since it presents itself as a possibility of facing the difficulties that present themselves. This is a bibliographical study. It is concluded that public consortia can be a viable and advantageous alternative in the search for joining efforts to solve common problems to meet PNRS requirements since they have advantages in all dimensions related to the principle of systemic vision.

O avanço dos meios de comunicação abre perspectiva para a participação cidadã e para o pleno exercício do direito à informação. Tal prerrogativa, contudo, depende da implementação das premissas legais relativas a tal direito expressas na Constituição Federal de 1988 e nas leis que a seguiram. A internet possibilita aos Comitês de Bacias Hidrográficas serem protagonistas da consecução do direito à informação, de modo a estimular a participação cidadã no debate público sobre a questão hídrica. Este estudo analisa a informação hídrica veiculada pelos comitês de bacias hidrográficas do RS no ambiente virtual, a fim de discutir de que modo suas práticas comunicativas atendem aos pressupostos legais do direito à informação. O estudo é descritivo e analítico, quanti-qualitativo, de base bibliográfica e documental. A amostra incluiu 25 comitês de bacias hidrográficas do Rio Grande do Sul, onde foram analisadas três categorias: acesso, informação ofertada e redes sociais.

As Tecnologias de Informação e Comunicação (TICs) constroem cenários novos e complexos, que medeiam processos de ensino e de aprendizagem. A partir desse panorama e apoiando-se na educomunicação, realizou-se a produção de um audiovisual com alunos da rede pública de ensino da Escola Estadual de Ensino Médio Paverama/RS. No processo de construção do audiovisual participaram agricultores do município de Paverama e um professor. O objetivo é verificar proximidades e potencialidades possíveis entre a educomunicação e o ensino de história. A pesquisa tem viés metodológico e intervencionista, caracterizando-se como aplicada, bibliográfica, documental e de campo. Explora-se a pesquisa-ação e o olhar etnográfico através de diários de campo. A produção audiovisual demonstrou que é possível criar e compartilhar conhecimento através da educomunicação, que a criação de ecossistemas comunicativos abre um leque de possibilidades e que a experimentação audiovisual pode dinamizar aprendizagens.

This paper seeks to understand the relations between the human being and the environment where he/she is inserted, starting from an analysis focusing the Upper-Uruguay region, in the state of Rio Grande do Sul, Brazil. We adopt the point of view that men and nature are essentially interrelated, being both of them active. Thus, beyond men interfer in the environment, nature is also in constant motion, intervenes and transforms. We will analyze the movements of the european settlers to adapt the environment to their needs and the effects of interactions with the environment over the perception of it. To achieve this purpose, it will be identified the grants given to the european settlers, changes provoked in natural landscape and the consequences in the perception of descendants who inhabit the region today. The research method is composed by two parts: ethnographic and documentary research., Este artigo apresenta análises históricas e ambientais a partir de um estudo documental e etnográfico realizado na região denominada Alto Uruguai, Rio Grande do Sul, Brasil. Este trabalho partiu da perspectiva da história ambiental a fim de contextualizar as mudanças e transformações na paisagem da região do Alto Uruguai, identificando os incentivos aos colonizadores europeus, as alterações na fisionomia natural e os reflexos na atual percepção dos moradores da região. A metodologia da pesquisa teve inspiração etnográfica. Para realizá-la, usou-se a pesquisa exploratória e analítica em documentos coletados no Arquivo Histórico Juarez Illa Font, da cidade de Erechim. Após a análise do material descrito, ensaios etnográficos foram realizados com alguns agricultores locais. Os dados da pesquisa foram destinados à compreensão de ações e do comportamento sociocultural e da relação do homem com a natureza. As conclusões apontam que a fisionomia natural da região do Alto Uruguai foi modificada de forma acelerada durante o início do processo de colonização (1900 a 1960). Os ideais políticos do período da colonização e a relação de domínio sobre a natureza dos primeiros colonizadores estão enraizados na cultura local e vêm influenciando a relação dos atuais moradores com o meio ambiente, o que confirma a necessidade de um trabalho de educação ambiental na região.