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Background: It is well known that the best nutritional option for infants is human milk, and that when breastfeeding is not possible, human milk banks are a possible alternative. However, in the case of infants with fat transport disorder like chylothorax, defatting of human milk is mandatory. Research Aim: The aim of the study was to reduce milk fat content without reducing other nutrients, increasing oxidative stress, or introducing harmful microorganisms. Methods: In this prospective, cross-sectional, observational study, we examined the influence of defatting and pasteurization of 50 donor samples on fat, macro- and micronutrients, as well as on oxidative stress markers. Results: Low-temperature centrifugation proved to be very efficient in defatting, reducing the concentration of triglycerides by 85% and cholesterol by 50%. The macronutrients (proteins, albumin, and Immunoglobulin A) did not undergo significant changes due to defatting and pasteurization procedures, while iron decreased by 36%. However, as the majority of iron is retained, this result does not remarkably change the milk composition. Furthermore, oxidative stress markers and antioxidant levels were unchanged, and the milk result was microbiologically safe. Conclusions: Cold milk centrifugation proved to be an effective technique that allows the reduction of human milk lipids. The determination of triglycerides and cholesterol can be used as an indicator of skimming. This procedure is not accompanied by substantial modifications of other components present in the milk.

Biosensors detect specific bio-analytes by generating a measurable signal from the interaction between the sensing element and the target molecule. Surface acoustic wave (SAW) biosensors offer unique advantages due to their high sensitivity, real-time response capability, and label-free detection. The typical SAW modes are the Rayleigh mode and the shear-horizontal mode. Both present pros and cons for biosensing applications and generally need different substrates and device geometries to be efficiently generated. This study investigates and characterizes ultra-high-frequency (UHF-) SAW resonator biosensors. It reveals the simultaneous presence of the two typical SAW modes, clearly separated in frequency, called slow and fast. The two modes are studied by numerical simulations and biosensing experiments with the glial-fibrillary-acidic-protein (GFAP) biomarker. The slow mode is generally more sensitive to changes in surface properties, such as temperature and mass changes, by a factor of about 1.4 with respect to the fast mode.

The analysis of the time profile of electrical signals produced by energy depositions in germanium detectors allows discrimination of events with different topologies. This is especially relevant for experiments searching for the neutrinoless double beta decay of $$^{76}$$ 76 Ge to distinguish the sought-after signal from other background sources. The standard calibration procedures used to tune the selection criteria for double-beta decay events use a $$^{228}$$ 228 Th source, because it provides samples of signal-like events. These samples exhibit energy spatial distributions with subtle different topologies compared to neutrinoless double-beta decay events. In this work, we will characterize these topological differences and, with the support of a $$^{56}$$ 56 Co source, evaluate biases and precision of calibration techniques which use such event samples. Our results will be particularly relevant for future experiments in which a solid estimation of the efficiency is required.

We evaluate the discovery probability of a combined analysis of proposed neutrinoless double-beta decay experiments in a scenario with normal ordered neutrino masses. The discovery probability strongly depends on the value of the lightest neutrino mass, ranging from zero in case of vanishing masses and up to 80-90\% for values just below the current constraints. We study the discovery probability in different scenarios, focusing on the exciting prospect in which cosmological surveys will measure the sum of neutrino masses. Uncertainties in nuclear matrix element calculations partially compensate each other when data from different isotopes are available. Although a discovery is not granted, the theoretical motivations for these searches and the presence of scenarios with high discovery probability strongly motivates the proposed international, multi-isotope experimental program.

Background: The objective of this study was to establish the age and sex-dependent reference intervals for coagulation assays evaluated in healthy children, ranging from 0 days to 16 years old. Methods: PT, aPTT, Fibrinogen (functional), Antithrombin activity, Protein C anticoagulant activity, Protein S free antigen, Thrombin time, D-Dimer, Von Willebrand Factor antigen, Lupus anticoagulant (screening), extrinsic and intrinsic pathway factors, and activated Protein C resistance were evaluated using STA-R Max2. Results: A total of 1280 subjects (671 males and 609 females) were divided into five groups, according to their age: 0–15 days (n = 280, 174 M and 106 F), 15–30 days (n = 208, 101 M and 107 F), 1–6 months (n = 369, 178 M and 191 F), 6–12 months (n = 214, 110 M and 104 F), and 1–16 years (n = 209, 108 M and 101 F). The 95% reference intervals and the 90% CI were established using the Harrell–Davis bootstrap method and the bootstrap percentile method, respectively. Conclusions: The present study supports the concept that adult and pediatric subjects should be evaluated using different reference intervals, at least for some coagulation tests, to avoid misdiagnosis, which can potentially lead to serious consequences for patients and their families, and ultimately the healthcare system.

A rationally designed gold-functionalized surface capable of capturing a target protein is presented using the biotin–streptavidin pair as a proof-of-concept. We carried out multiscale simulations to shed light on the binding mechanism of streptavidin on four differently biotinylated surfaces. Brownian Dynamics simulations were used to reveal the preferred initial orientation of streptavidin over the surfaces, whereas classical molecular dynamics was used to refine the binding poses and to investigate the fundamental forces involved in binding, and the binding kinetics. We assessed the binding events and the stability of the streptavidin attachment through a quartz crystal microbalance with dissipation monitoring (QCM-D). The sensing element comprises of biotinylated polyethylene glycol chains grafted on the sensor’s gold surface via thiol-Au chemistry. Finally, we compared the results from experiments and simulations. We found that the confined biotin moieties can specifically capture streptavidin from the liquid phase and provide guidelines on how to exploit the microscopic parameters obtained from simulations to guide the design of further biosensors with enhanced sensitivity.

SPIDER is the full-scale prototype of the ion source of the ITER Heating Neutral Beam Injector, where negative ions of Hydrogen or Deuterium are produced by a RF generated plasma and accelerated with a set of grids up to ~100 keV. The Power Supply System is composed of high voltage dc power supplies capable of handling frequent grid breakdowns, high current dc generators for the magnetic filter field and RF generators for the plasma generation. During the first 3 years of SPIDER operation different electrical issues were discovered, understood and addressed thanks to deep analyses of the experimental results supported by modelling activities. The paper gives an overview on the observed phenomena and relevant analyses to understand them, on the effectiveness of the short-term modifications provided to SPIDER to face the encountered issues and on the design principle of long-term solutions to be introduced during the currently ongoing long shutdown., 8 pages, 12 figures. Presented at SOFT 2022

At the International Thermonuclear Experimental Reactor (ITER) Neutral Beam Test Facility in Padua (Italy), the ion source full-scale prototype--SPIDER--has been in operation since June 2018 and, as of March 2021, has begun to explore the range of beam energies exceeding 30 keV. This has revealed a number of novel issues with the power supplies of ion source and extractor, mainly associated with grid breakdowns. Those power supplies include a set of high current single quadrant resonant converters, known as ion source support power supplies (ISSSs) and a high voltage pulse step modulator feeding the extraction gap. Grid breakdowns at beam energies exceeding 30 keV have shown the capability to upset the output measurements of the ISSSs, limiting operation and reducing reliability of the whole system. This article describes work undertaken first to identify the issues, found to belong to two distinct categories: general failures of all ISSSs measurements and trips from the output voltage measurements of the extraction gap power supply. Further investigation has associated the failures of the ISSS measurements to transient voltage affecting the electronics during grid breakdowns. A number of modifications to both power circuit and electronics have been undertaken and successfully tested, achieving immunity down to the full SPIDER operating voltage (-96 kV). The extraction gap power supply overvoltage has been quantified and a temporary modification deployed, able to mitigate it significantly. A deeper redesign will be required in this case and is planned during the 2022 shutdown of the experiment.

Counterintuitive experimental evidences have been observed during High Voltage Direct Current (HVDC) tests of two concave, axial-symmetric, electrodes insulated by large vacuum gaps of 3 and 7 cm with voltages from 150 to 370 kVdc. The dissipation of microdischarge power during the conditioning procedure occurs mostly on the anodic side in a region close to the axis of the system where the electric field is at a minimum, far from the positions where the breakdowns have been observed. The analyses of the phenomena are carried out by comparing the temporal evolution of voltages, currents, pressure, measurements of x-ray energy spectra, and images from infrared and visible light cameras. Numerical simulations, based on ray-tracing algorithm, correctly identify the positions where the power dissipation of microdischarges occurs. A mutual exchange of charged particles in the electrostatic field between electrodes seems a reasonable physical mechanism to interpret the observations. These findings suggest a new perspective to review the current literature and interpret new results considering geometric details which were so far omitted: the areas with the most intense electric field, typically located on the surfaces of the electrodes under test, are not necessarily the sole surfaces involved in the HVDC conditioning in high vacuum.

This paper shows the optical photoresponse in the IR-Vis-UV range of a AlN-based piezoelectric surface acoustic wave (SAW) delay-line device. The piezoelectric aluminum nitride (AlN) thin film has been sputtered on both silicon rigid substrate and flexible polyethylene naphthalate (PEN) substrate. Both devices have been investigated in their electroacoustic response, by measuring the transfer function S-21 and by laser Doppler vibrometer characterization. The silicon based SAW devices, stimulated by the IR-Vis-UV light, are strongly affected in the out-of-band insertion loss due to the photovoltaic effect. A mathematical model has been implemented to correlate the out-of-band loss with the material's electrical admittance change. In contrast PEN based SAW devices, due to the polymeric nature of the substrate, did not show any variation in the out-of-band loss. Moreover, when exposed to UV light, a frequency downshift of the Rayleigh and Lamb resonances modes have been observed in all the devices, due screening of the photoinduced electrons in the AlN piezoelectric layer which induces an acoustic wave velocity reduction. To the best of our knowledge, this is the first photoresponse study exploiting SAW in the range IR-Vis-UV, suggesting a new detection mode of UV light by a flexible AlN based SAW device. Further development of these devices can lead to a new class of light sensors from UV to IR, based on remote SAW devices.

High-energy astrophysical neutrinos, discovered by IceCube, are now regularly observed, albeit at a low rate due to their low flux. As a result, open questions about high-energy neutrino astrophysics and particle physics remain limited by statistics at best, or unanswered at worst. Fortunately, this situation will improve soon: in the next few years, a host of new neutrino telescopes, currently under planning and construction, will come online. It is natural to combine their collected observing power: we propose the Planetary Neutrino Monitoring System (PLE$\nu$M), a concept for a global repository of high-energy neutrino observations, in order to finally give firm answers to open questions. PLE$\nu$M will reach up to four times the exposure available today by combining the exposures of current and future neutrino telescopes distributed around the world -- IceCube, IceCube-Gen2, Baikal-GVD, KM3NeT, and P-ONE. Depending on the declination and spectral index, PLE$\nu$M will improve the sensitivity to astrophysical neutrinos by up to two orders of magnitude. We present first estimates on the capability of PLE$\nu$M to discover Galactic and extragalactic sources of astrophysical neutrinos and to characterize the diffuse flux of high-energy neutrinos in unprecedented detail., Comment: Presented at the 37th International Cosmic Ray Conference (ICRC 2021). 8 pages, 5 figures

Many approaches to diagnostic testing remain decades old. Well-established biosensing technologies (e.g., enzyme-linked immunosorbent assays and radio-immunoassays) typically cannot fulfill the requirements of portability and ease of use necessary for point-of-care purposes. Several alternatives have been proposed (e.g., quartz-crystal-microbalances, electrochemical sensors, cantilevers, and surface-plasmon-resonance sensors) but often lack high performance or still necessitate bulk ancillary instruments to operate. Here we present a highly sensitive, versatile, and easily integrable microfluidic lab-on-a-chip (LoC) for biosensing, fully based on surface acoustic waves (SAWs). By using ultra-high-frequency resonator-biosensors, we show that it is possible to perform highly sensitive assays in complex media. This all-electrical readout platform is benchmarked with the biotin-streptavidin binding in presence of non-specific binding proteins (serum albumin) at physiological concentration. The benchmark experiments were performed with the idea of mimicking a biological fluid, in which other molecular species at high concentration are present together with the analytes. We demonstrate that this LoC can detect sub-nanomolar concentrations of analytes in complex media. As a comparison with similar acoustic-wave-based systems, this full-SAW platform outperforms the standard commercial gravimetric sensors (i.e., quartz-crystal-microbalances) and the more common Love-SAW biosensors. This full-SAW LoC could be further developed for the detection of biomarkers in biological fluids.

Gorham-Stout disease (GSD) is a very rare disease characterized by increased bone erosion with angiomatous proliferation. The mechanisms underlying this disorder have not been deeply investigated. Due to its rarity, no guidelines are currently available for treatment and management of GSD. We recently evaluated the cellular alterations of the bone remodeling in patients showing that osteoclast precursors displayed increased ability to differentiate into osteoclasts and that affected osteoclasts resorb bone more actively than control cells. Moreover, osteoblasts isolated from a patient showed a defective ability to form mineralized nodules. In this paper, we investigated the molecular pathways involved in the cellular defects of GSD bone cells. For this study, we recruited nine patients and performed miRNome analysis of bone cells. Between the 178 miRNAs robustly expressed in GSD osteoclasts, significant modulation of three miRNAs (miR-1246, miR-1-3p, and miR-137-3p) involved in the regulation of osteoclast formation and activity or in the angiomatous proliferation was found in patients' cells. Interestingly, miR-1246 was also up-regulated in serum exosomes from patients. Analysis of miRNAs from patient osteoblasts suggested alteration of miR-204a-5p, miR-615-3p and miR-378a-3p regulating osteoblast function and differentiation. The resulting miRNA pattern may help to understand better the mechanisms involved in GSD and to identify new potential therapeutic targets for this rare disease.

To reach fusion conditions and control the plasma configuration in ITER, the next step in tokamak fusion research, two neutral beam injectors (NBIs) will supply 16.5 MW each, by neutralizing accelerated negative hydrogen or deuterium ions. The requirements of ITER NBIs (40A/1 MeV D- ions for ≤1 h, 46A/870 keV H- ions for ≤1000 s) have never been simultaneously attained. So in the Neutral Beam Test Facility (NBTF, Consorzio RFX, Italy) the operation of the full-scale ITER NBI prototype (MITICA) will be tested and optimised up to full performances, focussing on accelerator (including voltage holding), beam optics, neutralisation, residual ion removal. The NBTF includes also the full-scale prototype of the ITER NBI source with 100 keV particle energy (SPIDER), for early investigation of: negative ion production and extraction, source uniformity, negative ion current density and beam optics. This paper will describe the main results of the first two years of SPIDER operation, devoted to characterizing plasma and beam parameters, including investigation of RF-plasma coupling efficiency and magnetic filter field effectiveness in reducing co-extracted electrons. SPIDER is progressing towards the first caesium injection, which aims at increasing the negative ion density. A major shutdown, planned for 2021, to solve the issues identified during the operation and to carry out programmed modifications, will be outlined. The installation of each MITICA power supply and auxiliary system is completed; in-vessel mechanical components are under procurement by Fusion for Energy (F4E). Integration, commissioning and test of the power supplies, procured by F4E and QST, as the Japanese Domestic Agency (JADA), will be presented. In particular, 1.0MV insulating tests were carried out step-by-step and successfully completed. In 2020 integrated tests of the power supplies on the accelerator dummy load started, including the assessment of their resilience to accelerator grid breakdowns using a short-circuit device located in vacuum. The aggressive programme, to validate the NBI design at NBTF and to meet ITER schedule (requiring NBIs in operation in 2032), will be outlined. Unfortunately, in 2020 the coronavirus disease infection affected the NBTF activities. A solution to proceed with integrated power tests despite the coronavirus is presented.

SPIDER, the full-size prototype of the negative ion source for future ITER Heating and Current Drive, is equipped with a tomographic diagnostic to characterize the negative hydrogen (and deuterium) beam. The main goal of the tomographic system is the reconstruction of the beam emission pattern, which is proportional to the beam density, by assuming a uniform background gas, through the measurement of the beam emitted light along a sufficiently large number of lines-of-sight (LoSs), to study the beam homogeneity and divergence. Currently, 11 2D visible cameras observe the beam on a plane at a distance of 400 mm from the last grid of the accelerator, all around the beam; sensors of different sizes with different lenses are used, to maximize the resolution despite the limited number of points of view. The cameras are calibrated both in intensity and spatially and their fans of LoSs have been reconstructed. Once calibrated, the experimental data are compared with the simulated ones, simultaneously for all the cameras in order to have a complete profile of the extracted beam. Simultaneous Algebraic Reconstruction Technique (SART) algorithm as inversion technique is used to reconstruct for the first time the 2D beam emissivity profile, and it is applied to study the spatial uniformity of the beam as a function of the filter field and the extraction voltage.

Neutral beam injection (NBI) systems are required for heating and current drive in the next generation fusion experiment ITER, and strict requirements are placed on the beamlet core divergence (

The GERmanium Detector Array (GERDA) collaboration searched for neutrinoless double-$\beta$ decay in $^{76}$Ge with an array of about 40 high-purity isotopically-enriched germanium detectors. The experimental signature of the decay is a monoenergetic signal at Q$_{\beta\beta}$ = 2039.061(7)keV in the measured summed energy spectrum of the two emitted electrons. Both the energy reconstruction and resolution of the germanium detectors are crucial to separate a potential signal from various backgrounds, such as neutrino-accompanied double-$\beta$ decays allowed by the Standard Model. The energy resolution and stability were determined and monitored as a function of time using data from regular $^{228}$Th calibrations. In this work, we describe the calibration process and associated data analysis of the full GERDA dataset, tailored to preserve the excellent resolution of the individual germanium detectors when combining data over several years., Comment: 12 pages, 7 figures

Glial-fibrillary-acidic-protein (GFAP) has recently drawn significant attention from the clinical environment as a promising biomarker. The pathologies which can be linked to the presence of GFAP in blood severely affect the human central nervous system. These pathologies are glioblastoma multiforme (GBM), traumatic brain injuries (TBIs), multiple sclerosis (MS), intracerebral hemorrhage (ICH), and neuromyelitis optica (NMO). Here, we develop three different detection strategies for GFAP, among the most popular in the biosensing field and never examined side by side within the experimental frame. We compare their capability of detecting GFAP in a clean-buffer and serum-matrix by using gold-coated quartz-crystal-microbalance (QCM) sensors. All the three detection strategies are based on antibodies, and each of them focuses on a key aspect of the biosensing process. The first is based on a polyethylene glycol (PEG) chain for antifouling, the second on a protein-G linker for controlling antibody-orientation, and the third on antibody-splitting and direct surface immobilization for high-surface coverage. Then, we select the best-performing protocol and validate its detection performance with an ultra-high-frequency (UHF) surface-acoustic-wave (SAW) based lab-on-chip (LoC). GFAP successful detection is demonstrated in a clean-buffer and serum-matrix at a concentration of 35 pM. This GFAP level is compatible with clinical diagnostics. This result suggests the use of our technology for the realization of a point-of-care biosensing platform for the detection of multiple brain-pathology biomarkers.

Gorham-Stout disease (GSD) is a very rare syndrome displaying excessive bone erosion and vascular lesion. Due to the rarity of the disease and to the limited studies, its etiopathogenesis is not entirely known. The involvement of immune system in the progressive osteolysis was recently suggested. Indeed, extensive reciprocal interactions between the immune and skeletal systems have been demonstrated. This study aimed to evaluate alterations of immune cells in GSD. An increase of CD8+ cells and reduction of CD4+ and CD4+CD25+CD127low cells was revealed in patients. Interestingly, patients’ regulatory T cells maintain the ability to respond to extracellular stimuli and to regulate osteoclastogenesis; GSD cells proliferate under aCD3/CD28 signal reaching similar levels to those observed in control culture and exert their immunomodulatory activity on effector T cells. GSD Treg cells preserved their inhibitory effects on the osteoclastogenesis. These results suggest that stimulation of Treg cells could open the way for the identification and testing of new therapeutic approaches for patients affected by GSD.

We discuss the impact of the cosmological measurements on the predictions of the Majorana mass of the neutrinos, the parameter probed by neutrinoless double-beta decay experiments. Using a minimal set of assumptions, we quantify the probabilities of discovering neutrinoless double-beta decay and introduce a new graphical representation that could be of interest for the community.

The GERmanium Detector Array (GERDA) experiment searched for the lepton-number-violating neutrinoless double-$\beta$ ($0\nu\beta\beta$) decay of $^{76}$Ge, whose discovery would have far-reaching implications in cosmology and particle physics. By operating bare germanium diodes, enriched in $^{76}$Ge, in an active liquid argon shield, GERDA achieved an unprecedently low background index of $5.2\times10^{-4}$ counts/(keV$\cdot$kg$\cdot$yr) in the signal region and met the design goal to collect an exposure of 100 kg$\cdot$yr in a background-free regime. When combined with the result of Phase I, no signal is observed after 127.2 kg$\cdot$yr of total exposure. A limit on the half-life of $0\nu\beta\beta$ decay in $^{76}$Ge is set at $T_{1/2}>1.8\times10^{26}$ yr at 90% C.L., which coincides with the sensitivity assuming no signal., Comment: 7 pages, 3 figures, submitted to Physical Review Letters

The Standard Model of Particle Physics predicts the double-$\beta$ decay of certain nuclei with the emission of two active neutrinos. In this letter, we argue that double-$\beta$ decay experiments could be used to probe models with light exotic fermions through the search for spectral distortions in the electron spectrum with respect to the Standard Model expectations. We consider two concrete examples: models with light sterile neutrinos, singly produced in the double-$\beta$ decay, and models with a light $Z_2$-odd fermion, pair produced due to a $Z_2$ symmetry. We estimate the discovery potential of a selection of double-$\beta$ decay experiments and find that future searches will test for the first time a new part of the parameter space of interest at the MeV-mass scale., Comment: 9 pages, 3 figures. Matches published version

Surface acoustic waves (SAWs) have the potential to become the basis for a wide gamut of lab-on-a-chips (LoCs). These mechanical waves are among the most promising physics that can be exploited for fulfilling all the requirements of commercially appealing devices that aim to replace–or help–laboratory facilities. These requirements are low processing cost of the devices, scalable production, controllable physics, large flexibility of tasks to perform, easy device miniaturization. To date, SAWs are among the small set of technologies able to both manipulate and analyze biological liquids with high performance. Therefore, they address the main needs of microfluidics and biosensing. To this purpose, the use of high-frequency SAWs is key. In the ultra-high-frequency regime (UHF, 300 MHz—3 GHz) SAWs exhibit large sensitivities to molecule adsorption and unparalleled fluid manipulation capabilities, together with overall device miniaturization. The UHF-SAW technology is expected to be the realm for the development of complex, reliable, fully automated, high-performance LoCs. In this review, we present the most recent works on UHF-SAWs for microfluidics and biosensing, with a particular focus on the LoC application. We derive the relevant scale laws, useful formulas, fabrication guidelines, current limitations of the technology, and future developments.

We present the first search for bosonic superweakly interacting massive particles (super-WIMPs) as keV-scale dark matter candidates performed with the GERDA experiment. GERDA is a neutrinoless double-s decay experiment which operates high-purity germanium detectors enriched in ^{76}Ge in an ultralow background environment at the Laboratori Nazionali del Gran Sasso (LNGS) of INFN in Italy. Searches were performed for pseudoscalar and vector particles in the mass region from 60 keV/c^{2} to 1 MeV/c^{2}. No evidence for a dark matter signal was observed, and the most stringent constraints on the couplings of super-WIMPs with masses above 120 keV/c^{2} have been set. As an example, at a mass of 150 keV/c^{2} the most stringent direct limits on the dimensionless couplings of axionlike particles and dark photons to electrons of g_{ae}

The GERDA experiment investigates lepton-number violation and whether neutrinos have a Majorana nature by searching for a rare nuclear transition called neutrinoless double beta decay (0vbb). The statistical challenges that arise in the search for rare-processes: evaluating the discovery power, the limit setting power on the signal rate and the goodness of fit of the background for models with data sets consisting of a small number of events are discussed. The topics selected include the systematic variations in the results due to the choice of fit models. This is investigated through Bayesian Hierarchical modelling. Order statistics and the Bayes factor are used as tools to study the background goodness of fit and the signal discovery power.

We report on an improved measurement of the $^8$B solar neutrino interaction rate with the Borexino experiment at the Laboratori Nazionali del Gran Sasso. Neutrinos are detected via their elastic scattering on electrons in a large volume of liquid scintillator. The measured rate of scattered electrons above 3 MeV of energy is $0.223\substack{+0.015 \\ -0.016}\,(stat)\,\substack{+0.006 \\ -0.006}\,(syst)$ cpd/100 t, which corresponds to an observed solar neutrino flux assuming no neutrino flavor conversion of $\Phi\substack{\rm ES \\ ^8\rm B}=2.57\substack{+0.17 \\ -0.18}(stat)\substack{+0.07\\ -0.07}(syst)\times$10$^6$ cm$^{-2}\,$s$^{-1}$. This measurement exploits the active volume of the detector in almost its entirety for the first time, and takes advantage of a reduced radioactive background following the 2011 scintillator purification campaign and of novel analysis tools providing a more precise modeling of the background. Additionally, we set a new limit on the interaction rate of solar $hep$ neutrinos, searched via their elastic scattering on electrons as well as their neutral current-mediated inelastic scattering on carbon, $^{12}$C($\nu,\nu'$)$^{12}$C* ($E_{\gamma}$= 15.1 MeV).

For the ITER fusion experiment, two neutral beam injectors are required for plasma heating and current drive. Each injector supplies a power of about 17 MW, obtained from neutralization of 40 A (46 A), 1 MeV (0.87 MeV) negative deuterium (hydrogen) ions. The full beam is composed of 1280 beamlets, formed in 16 beamlet groups, and strict requirements apply to the beamlet core divergence (

The Borexino liquid scintillator neutrino observatory has a unique capability to perform high-precision solar neutrino observations thanks to its exceptional radiopurity and good energy resolution (5% at 1 MeV). A comprehensive study of the pp-chain neutrinos was presented that includes the direct measurements of 7Be, pp and pep neutrino fluxes with the highest precision ever achieved (down to 2.8% in the 7Be component), the 8B with the lowest energy threshold, the best limit on CNO neutrinos and the first Borexino limit on hep neutrinos. These results are important to validate the MSW-LMA oscillation paradigm across the full solar energy range and to exclude possible Non-Standard neutrino Interactions (NSIs). In particular the effects of neutrino-flavor-diagonal Neutral-Current (NC) interactions that modify the vee and vτe couplings while preserving their chiral and flavor structures, have been investigated. At detection, the shape of the electron-recoil spectrum is affected by changes in the vee and vτe couplings, quantified by the parameters ε e L / R and ε τ L / R . New bounds to all four parameters were obtained, quite stringent compared to the global ones. In particular, the best constraint to-date on ε e L was achieved. A comprehensive summary of all the recent results on solar neutrinos from Borexino is reported in the present paper.

The dynamics of turbulent filaments in L and ELM-free H-mode in the RFX-mod device operating as a tokamak is discussed in the present work. L-H transition and sustained H-mode have been achieved in the RFX-mod with the aid of an external electrode biasing (Spolaore et al 2017 Nucl. Fusion 57 116039). Through advanced statistical techniques, turbulent filaments are detected and tracked from the edge to the scrape-off layer (SOL) in a two-dimension floating potential map (measured with a 2D array of Langmuir probes) related with extreme events of a fixed ion saturation current signal in the SOL. While in L-mode filaments travel almost freely, their motion becomes restricted to the near SOL during the induced biasing H-mode. In this region, the background shear decorrelation time becomes shorter than the convective filament time, favouring its suppression. However, the experimental observation of a nearly 'trapped' monopole potential points out the possible role of the vortex selection mechanism. The sign and magnitude of the turbulent parallel vorticity are consistent with the E × B background vorticity in the SOL in this regime, while in L-mode, the inferred turbulent vorticity is always above the background one. The sparse filamentary transport in the far SOL during H-mode is further confirmed from the measurements of a poloidally symmetric array of electrostatic sensors on the wall. Lastly, the radial velocity of filaments in both regimes was discussed in the framework of analytical scalings. Filaments in L-mode exhibit a reasonable agreement with the sheath-limited regime in the cold ion approximation, with a non-negligible associated parallel current density measured with a set of 3-axial pick-up coils installed in the probe head. In H-mode, the collisionality increases near the separatrix while filaments seem to follow the inertial regime before become trapped.

A thermal calorimetric apparatus was designed, built and calibrated for measuring the activity of the artificial 144 Ce —144 Pr antineutrino source. This measurement will be performed at the Laboratori Nazionali del Gran Sasso in Italy, just before the source insertion in the tunnel under the Borexino detector and a precision better than 1% is required for a disappearance technique measurement in the SOX (Short distance neutrino Oscillation with BoreXino) project. In this work the apparatus is described and the most important results from the calibration measurements are shown, where the final precision of few per thousand is demonstrated.

The NIO1 (Negative Ion Optimization phase 1) source can provide continuous beam operation, which is convenient for systematic parameter and equipment studies. Even in the pure volume production regime, the source yield was found to depend on conditioning procedures. Magnetic configuration tests continued adding magnets to the existing setup; the filter field component Bx has been progressively extended to span the −12 to 5 mT range, and as a trend, source performances improved with |Bx|. The progress of camera beam diagnostics and of the quality of the volume-produced H− beam is also shown. The status, off-line results, and reliability of a first NIO1 cesium oven are discussed; other upgrades in preparation (cavity ring down spectrometer, the end calorimeter, and conceptual tests of the energy recovery system) are also listed.The NIO1 (Negative Ion Optimization phase 1) source can provide continuous beam operation, which is convenient for systematic parameter and equipment studies. Even in the pure volume production regime, the source yield was found to depend on conditioning procedures. Magnetic configuration tests continued adding magnets to the existing setup; the filter field component Bx has been progressively extended to span the −12 to 5 mT range, and as a trend, source performances improved with |Bx|. The progress of camera beam diagnostics and of the quality of the volume-produced H− beam is also shown. The status, off-line results, and reliability of a first NIO1 cesium oven are discussed; other upgrades in preparation (cavity ring down spectrometer, the end calorimeter, and conceptual tests of the energy recovery system) are also listed.

Borexino is a 280-ton liquid scintillator detector located at the Laboratori Nazionali del Gran Sasso (LNGS), Italy and is one of the two detectors that has measured geoneutrinos so far. The unprecedented radio-purity of the scintillator, the shielding with highly purified water, and the placement of the detector at a 3800 m w.e. depth have resulted in very low background levels and have made Borexino an excellent apparatus for geoneutrino measurements. The analysis techniques of the latest geoneutrino results with Borexino were presented using the data obtained from December 2007 to April 2019, corresponding to an exposure of (1.12 ± 0.05) × 1032 protons × yr. Enhanced analysis techniques, such as an increased fiducial volume, improved veto for cosmogenic backgrounds, extended energy and coincidence time windows, as well as a more efficient α/β particle discrimination have been adopted in this measurement. The updated statistics and these elaborate resulted in a geoneutrino signal of 47.0 − 7.7 + 8.4 ( stat ) − 1.9 + 2.4 ( sys ) TNU with − 17.2 + 18.3 % total precision.

Polyphenols are a family of compounds present in grapes, musts, and wines. Their dosage is associated with the grape ripening, correct must fermentation, and final wine properties. Owing to their anti-inflammatory properties, they are also relevant for health applications. To date, such compounds are detected mainly via standard chemical analysis, which is costly for constant monitoring and requires a specialized laboratory. Cheap and portable sensors would be desirable to reduce costs and speed up measurements. This paper illustrates the development of strategies for sensor surface chemical functionalization for polyphenol detection. We perform measurements by using a commercial quartz crystal microbalance with dissipation monitoring apparatus. Chemical functionalizations are based on proteins (bovine serum albumin and gelatin type A) or customized peptides derived from istatine-5 and murine salivary protein-5. Commercial oenological additives containing pure gallic tannins or proanthocyanidins, dissolved in water or commercial wine, are used for the analysis. Results indicate that selected functionalizations enable the detection of the two different tannin families, suggesting a relationship between the recorded signal and concentration. Gelatin A also demonstrates the ability to discriminate gallic tannins from proanthocyanidins. Outcomes are promising and pave the way for the exploitation of such devices for precision oenology.

In the last few decades, new types of cell cultures have been introduced to provide better cell survival and development, with micro- and nanoenvironmental physicochemical conditions aimed at mimicking those present in vivo. However, despite the efforts made, the systems available to date are often difficult to replicate and use. Here, an easy-to-use surface-acoustic-wave (SAW)-based platform is presented for realizing dynamic cell cultures that is compatible with standard optical microscopes, incubators, and cell-culture dishes. The SAW chip is coupled to a standard Petri dish via a polydimethylsiloxane (PDMS) disc and consists of a lithium niobate (LN) substrate on which gold interdigital transducers (IDTs) are patterned to generate the SAWs and induce acoustic streaming in the dish. SAW excitation is verified and characterized by laser Doppler vibrometry, and the fluid dynamics is studied by microparticle image velocimetry (?PIV). Heating is measured by an infrared (IR) thermal camera. We finally tested this device with the U-937 monocyte cell line for viability and proliferation and cell-morphological analysis. The data demonstrate that it is possible to induce significant fluid recirculation within the Petri dish while maintaining negligible heating. Remarkably, cell proliferation in this condition was enhanced by 36 ± 12% with respect to those of standard static cultures. Finally, we show that cell death does not increase and that cell morphology is not altered in the presence of SAWs. This device is the first demonstration that SAW-induced streaming can mechanically improve cell proliferation and further supports the great versatility and biocompatibility of the SAW technology for cell manipulation.

A label-free sub-nanomolar Rayleigh surface acoustic wave (R-SAW)-based resonator biosensor is demonstrated for biomolecular detection in liquid after drying. The biosensor comprises two interdigital transducers for R-SAW generation and two positive and negative reflectors to confine the acoustic energy in the sensitive area. We benchmark this biosensor against biotin-streptavidin binding, which is a standard, well-known model for a variety of biosensing processes. The experiments demonstrate a limit of detection of 104 pM and a normalized sensitivity of −296 m2 kg−1. As a comparison with similar acoustic-wave based systems, both sensitivity and limit of detection are better than that of standard commercial gravimetric sensors (i.e., quartz-crystal-microbalances) and generally better than that of more common Love-SAW biosensors. Our biosensor has a dynamic range potentially compatible with several health- and safety-related assays, among all cancer biomarker detection.

The SPIDER ion source has been designed to generate a current of H- ions up to 60 A and accelerate them up to 100 keV. In addition, the ratio between the co-extracted electrons and negative ions has to be below 1, and a beam inhomogeneity within 10 % has to be attained. A magnetic filter field is produced by a current flowing through the plasma electrode in the vertical direction with the aim of reducing electron temperature and density in the extraction region, so as to enhance the survival probability of H- ions while reducing the co-extracted electrons. Another strategy to reduce the co-extracted electrons is to apply a small bias voltage to the plasma grid with respect to the ion source body. In addition to this, a so-called bias plate is installed in the vicinity of the plasma grid, which can also be biased with respect to source body. However, the application of a bias voltage affects the beam inhomogeneity by modifying the ExB plasma drift within the ion source. Recently, the filter field configuration of SPIDER was modified so as to improve the plasma diffusion towards the extraction region. In addition, the voltage ratings of the bias power supplies were increased. Both these modifications permit to efficiently lower the electron co-extracted current. The present contribution describes such improvements while focusing on the effects they have on the beam inhomogeneity.

We demonstrate that megaton-mass neutrino telescopes are able to observe the signal from long-lived particles beyond the Standard Model, in particular the stau, the supersymmetric partner of the tau lepton. Its signature is an excess of charged particle tracks with horizontal arrival directions and energy deposits between 0.1 and 1 TeV inside the detector. We exploit this previously-overlooked signature to search for stau particles in the publicly available IceCube data. The data shows no evidence of physics beyond the Standard Model. We derive a new lower limit on the stau mass of 320 GeV (95% C.L.) and estimate that this new approach, when applied to the full data set available to the IceCube collaboration, will reach word-leading sensitivity to the stau mass (mτ˜=450GeV).

The GERmanium Detector Array (Gerda) experiment at the Gran Sasso underground laboratory (LNGS) of INFN is searching for neutrinoless double-beta (0νββ) decay of 76Ge. The technological challenge of Gerda is to operate in a “background-free” regime in the region of interest (ROI) after analysis cuts for the full 100 kg·yr target exposure of the experiment. A careful modeling and decomposition of the full-range energy spectrum is essential to predict the shape and composition of events in the ROI around Qββ for the 0νββ search, to extract a precise measurement of the half-life of the double-beta decay mode with neutrinos (2νββ) and in order to identify the location of residual impurities. The latter will permit future experiments to build strategies in order to further lower the background and achieve even better sensitivities. In this article the background decomposition prior to analysis cuts is presented for Gerda Phase II. The background model fit yields a flat spectrum in the ROI with a background index (BI) of $$ {16.04}_{-0.85}^{+0.78}\cdotp {10}^{-3} $$ 16.04 − 0.85 + 0.78 · 10 − 3 cts/(keV·kg·yr) for the enriched BEGe data set and $$ {14.68}_{-0.52}^{+0.47}\cdotp {10}^{-3} $$ 14.68 − 0.52 + 0.47 · 10 − 3 cts/(keV·kg·yr) for the enriched coaxial data set. These values are similar to the one of Phase I despite a much larger number of detectors and hence radioactive hardware components.

We compare the current statistical methods applied to short-baseline neutrino oscillation experiments looking for light sterile neutrinos. The interpretation of the reconstructed confidence regions differs because of the different definition of the alternative hypothesis in the hypothesis test. When the alternative hypothesis extends over the whole physical allowed parameter space of the oscillation parameters, proper confidence regions are obtained both in case of a limit and a signal. Since the different definition of the alternative hypothesis prevents from a direct comparison of the results, a standardized analysis is proposed.

We report on searches for neutrinos and antineutrinos from astrophysical sources performed with the Borexino detector at the Laboratori Nazionali del Gran Sasso in Italy. Electron antineutrinos ($\bar{\nu}_e$) are detected in an organic liquid scintillator through the inverse $\beta$-decay reaction. In the present work we set model-independent upper limits in the energy range 1.8-16.8 MeV on neutrino fluxes from unknown sources that improve our previous results, on average, by a factor 2.5. Using the same data set, we first obtain experimental constraints on the diffuse supernova $\bar{\nu}_e$ fluxes in the previously unexplored region below 8 MeV. A search for $\bar{\nu}_e$ in the solar neutrino flux is also presented: the presence of $\bar{\nu}_e$ would be a manifestation of a non-zero anomalous magnetic moment of the neutrino, making possible its conversion to antineutrinos in the strong magnetic field of the Sun. We obtain a limit for a solar $\bar{\nu}_e$ flux of 384 cm$^{-2}$s$^{-1}$ (90% C.L.), assuming an undistorted solar $^{8}$B neutrinos energy spectrum, that corresponds to a transition probability $p_{ \nu_e \rightarrow \bar\nu_{e}}$ 1.8 MeV. At lower energies, by investigating the spectral shape of elastic scattering events, we obtain a new limit on solar $^{7}$Be-$\nu_e$ conversion into $\bar{\nu}_e$ of $p_{ \nu_e \rightarrow \bar \nu_{e}}, Comment: 18 pages, 8 figures, 4 tables, 73 references

Borexino is running at the INFN Gran Sasso National Laboraries, Italy since 2007. Its technical distinctive feature is the unprecedented ultralow background of the inner scintillating core, which is the foundation for the outstanding achievements accumulated by this experiment. In this contribution, after recalling the main features of the detector, the impressive solar data gathered so far by Borexino will be summarized. Special emphasis will be given to the illustration of the recent release of the fluxes as stemming from the simultaneous real time spectroscopy of the neutrinos from the entire $pp$ nuclear fusion chain in the Sun, opening with the remarkable 2.7% accuracy of the ⁷Be flux the era of precision measurements also in the realm of the sub-MeV solar neutrinos. Such results put Borexino in the unique situation of being the only detector able to perform solar neutrino spectroscopy over the entire solar spectrum; the counterpart of this peculiar status, in the flavor conversion interpretation of the solar neutrino data, is the capability of Borexino alone to perform the full validation across the solar energy range of the MSW-LMA paradigm. This contribution will be concluded with an account of the Borexino accomplishments in the geo-neutrino field, marked by the detection of the geo-neutrino signal with a significance as high as $5.9\sigma$.