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Trabajo presentado al: 7th EAAP International Symposium on Energy and Protein Metabolism and Nutrition (ISEP 2022). In: Animal Science Proceedings. Vol. 13 (3), p. 410-411. Granada (Spain). 12-15 septiembre., Funding from the Spanish State Research Agency (AEI) and the European Regional Development Fund is gratefully acknowledged (Project AGL2016-75322-C2-2-R). Alba Martín gratefully acknowledges the receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033, El FSE invierte en tu futuro)

2 páginas.--Trabajo presentado al: 7th EAAP International Symposium on Energy and Protein Metabolism and Nutrition (ISEP 2022). In: Animal Science Proceedings., Project AGL2016-75322-C2-2-R from the Spanish Research Agency and the European Regional Development Fund.

Trabajo presentado al: 73rd Annual Meeting of the European Federation of Animal Science (EAAP). Oporto (Portugal), 5-9 de septiembre. 2022

Trabajo presentado al: 73rd Annual Meeting of the European Federation of Animal Science (EAAP), Oporto (Portugal), 5-9 de septiembre., Grant AGL2016-75322-C2-2-R funded by MCIN/AEI/ 10.13039/501100011033 and by the European Union ‘ERDF A way of making Europe'

This article explores the relationship between the growth of irrigation works, environmental change, and rural migration in western Mexico from 1940 to 1964. It begins by analyzing how Mexi...

7 páginas, 6 tablas., Early feed restriction of lambs promotes a permanent mitochondrial dysfunction that impairs β-oxidation of fatty acids along the whole life. Therefore, dietary L-carnitine might help to improve the mitochondrial function of these lambs, thus modifying lipid metabolism and meat quality traits. In order to test this hypothesis an experiment was carried out with 22 Merino lambs that were subjected to an early feed restriction during the suckling period. Once weaned, the lambs were allocated to a control group (CTRL, n = 11) being fed ad libitum a complete pelleted diet during the fattening phase, whereas the second group (CARN, n = 11) received the same diet formulated with 3 g/kg of L-carnitine. Carcass characteristics were not affected (P > 0.05) by treatment. However, lambs fed L-carnitine showed higher amounts of intramuscular fat (26.5 vs. 33.6 g/kg fresh matter; P = 0.047) with a lower ratio between polyunsaturated and saturated fatty acids (0.425 vs 0.333; P = 0.023) and a higher atherogenic (0.507 vs 0.597; P < 0.001) and thrombogenic index (1.23 vs 1.42; P < 0.001). An increase in lightness (P < 0.05) and a tendency to improved oxidative stability in cooked meat (P = 0.066) were also observed in the CARN group. Consequently, dietary L-carnitine supplied during the fattening period of early feed restricted lambs modifies meat quality traits thus increasing lightness, oxidative stability and intramuscular fat content, but worsening the fatty acid profile., This work was funded by Ministerio de Ciencia e Innovacio´n (RTI2018–099329-B-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”). Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019–089288) from Ministerio de Ciencia e Innovacio´n (MCIN/AEI/10.13039/501100011033, “El FSE invierte en tu futuro”).

Trabajo presentado al: 7th EAAP International Symposium on Energy and Protein Metabolism and Nutrition (ISEP 2022). In: Animal Science Proceedings. Vol. 13 (3), p. 418-419. Granada (Spain). 12-15 septiembre., Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033. ‘‘El FSE invierte en tu futuro”); This work was funded by Ministerio de Ciencia e Innovación (RTI2018-099329-B-I00. MCIN/AEI/10.13039/501100011033. ‘‘FEDER. Una manera de hacer Europa”).

Venous thromboembolism (VTE) is a serious complication in hematologic neoplasms, so finding adequate prevention strategies is an urgent requirement. However, prospective studies with large enough cohorts are scarce, limiting the development of evidence-based thromboprophylaxis guidelines. The present position paper is addressed to all hematologists treating patients affected by hematologic neoplasms with the aim to provide clinicians with a useful tool for the prevention of VTE.

This study was intended to determine the effect of adding flax seeds to a concentrate for lamb fattening on the fatty acid composition of the omental fat depot in Pramenka lambs reared indoors. A total of 63 lambs (10±3 kg of live body weight, 30±7 days of age, 30 males and 33 females) were used. They were divided into two groups: a control (CON) fed on hay, ewe’s milk, and a 300-g daily ration of a commercial concentrate, and an experimental group (FS) fed on hay, ewe’s milk and 300 g/day of the concentrate enriched with 5% of flax seeds. After a 60-day fattening period for each group, 10 lambs (5 males and 5 females) were selected and omental fat samples were analysed for fatty acid composition. Highly significant differences (p

Póster presentado en el International Sheep Veterinary Association (ISVA). Organized together with Sociedad Española de Ovinotécnia y Caprinotecnia (SEOC). 23-35 november. Virtual meeting., MCI/AEI/FEDER, EU; RTI2018-099329-B-100.

Genomic instability is a hallmark of cancer, and often is the result of altered DNA repair capacities in tumour cells. DNA damage repair defects are common in different cancer types; these alterations can also induce tumour-specific vulnerabilities that can be exploited therapeutically. In 2009, a first-in-man clinical trial of the poly(ADP-ribose) polymerase (PARP) inhibitor olaparib clinically validated the synthetic lethal interaction between inhibition of PARP1, a key sensor of DNA damage, and BRCA1/BRCA2 deficiency. In this review, we summarize a decade of PARP inhibitor clinical development, a work that has resulted in the registration of several PARP inhibitors in breast (olaparib and talazoparib) and ovarian cancer (olaparib, niraparib and rucaparib, either alone or following platinum chemotherapy as maintenance therapy). Over the past 10 years, our knowledge on the mechanism of action of PARP inhibitor as well as how tumours become resistant has been extended, and we summarise this work here. We also discuss opportunities for expanding the precision medicine approach with PARP inhibitors, identifying a wider population who could benefit from this drug class. This includes developing and validating better predictive biomarkers for patient stratification, mainly based on homologous recombination defects beyond BRCA1/BRCA2 mutations, identifying DNA repair deficient tumours in other cancer types such as prostate or pancreatic cancer, or by designing combination therapies with PARP inhibitors.

Invasive fusariosis has a global increasing incidence and has emerged as a severe infection in nonneutropenic patients., Invasive fusariosis (IF) is associated with severe neutropenia in patients with concurrent hematologic conditions. We conducted a retrospective observational study to characterize the epidemiology of IF in 18 Spanish hospitals during 2000–2015. In that time, the frequency of IF in nonneutropenic patients increased from 0.08 cases per 100,000 admissions in 2000–2009 to 0.22 cases per 100,000 admissions in 2010–2015. Nonneutropenic IF patients often had nonhematologic conditions, such as chronic cardiac or lung disease, rheumatoid arthritis, history of solid organ transplantation, or localized fusariosis. The 90-day death rate among nonneutropenic patients (28.6%) and patients with resolved neutropenia (38.1%) was similar. However, the death rate among patients with persistent neutropenia (91.3%) was significantly higher. We used a multivariate Cox regression analysis to characterize risk factors for death: persistent neutropenia was the only risk factor for death, regardless of antifungal therapy.

Hemostasis is crucial for reducing bleeding during surgical procedures. The points-of-care based on the platelet function test could be useful to minimize the complications related to chronic antiplatelet therapy during surgery. The present study is aimed at comparing two point-of-care platelet function devices-Platelet Function Analyzer PFA-100(R) (Siemens Canada, Mississauga, ON, Canada) and Plateletworks(R)(Helena Laboratories, Beaumont, TX, USA). Our objective is to evaluate if they provide comparable and useful information to manage anti-aggregate patients before surgery. We included patients with a femoral fracture receiving chronic antiplatelet therapy and a median age of 89 years (range from 70 to 98). A platelet function evaluation was performed on all patients before surgery using both devices-Plateletworks(R) and PFA-100(R). The correlation between Plateletworks(R) and PFA-100(R) was performed using Cohen's Kappa coefficient. Twenty consecutive patients participated in the trial; 16 patients were under treatment with 75 mg/day of clopidogrel, three with >300 mg/day of acetylsalicylic acid (ASA), and only one was in treatment with both antiplatelet agents. Cohen's Kappa coefficient was 0.327 comparing PFA-100(R)-ADP (adenosine diphosphate) and Plateletworks(R) and, 0.200 comparing PFA-100(R)-EPI (epinephrine) and Plateletworks(R). In conclusion, we found a weak concordance comparing PFA-100(R) and Plateletworks(R). This could partially be due to the advanced age of the included patients. However, given the limited sample size, more studies are necessary to confirm these results.

[No abstract available]

Trabajo presentado al: 71st Annual Meeting of the European Federation of Animal Science (EAAP), 239. 1-4 de diciembre, Virtual meeting (2020).

Treatment of carbapenemase‐producing Enterobacterales bloodstream infections in solid organ transplant recipients is challenging. The objective of this study was to develop a specific score to predict mortality in solid organ transplant recipients with carbapenemase‐producing Enterobacterales bloodstream infections. A multinational, retrospective (2004‐2016) cohort study (INCREMENT‐SOT, ClinicalTrials.gov NCT02852902) was performed. The main outcome variable was 30‐day all‐cause mortality. The INCREMENT‐SOT‐CPE score was developed using logistic regression. The global cohort included 216 patients. The final logistic regression model included the following variables: INCREMENT‐CPE mortality score ≥8 (8 points), no source control (3 points), inappropriate empirical therapy (2 points), cytomegalovirus disease (7 points), lymphopenia (4 points), and the interaction between INCREMENT‐CPE score ≥8 and CMV disease (minus 7 points). This score showed an area under the receiver operating characteristic curve of 0.82 (95% confidence interval [CI] 0.76‐0.88) and classified patients into 3 strata: 0‐7 (low mortality), 8‐11 (high mortality), and 12‐17 (very‐high mortality). We performed a stratified analysis of the effect of monotherapy vs combination therapy among 165 patients who received appropriate therapy. Monotherapy was associated with higher mortality only in the very‐high (adjusted hazard ratio [HR] 2.82, 95% CI 1.13‐7.06, P = .03) and high (HR 9.93, 95% CI 2.08‐47.40, P = .004) mortality risk strata. A score‐based algorithm is provided for therapy guidance., This work was supported by Plan Nacional de I+D+i 2013‐2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Ciencia, Innovación y Universidades, Spanish Network for Research in Infectious Diseases [REIPI RD16/0016/0008; RD16/0016/0001, RD16/0016/0002, RD16/0016/00010] ‐ co‐financed by European Development Regional Fund “A way to achieve Europe”, Operative program Intelligent Growth 2014‐2020; ESCMID Study Group for Infections in Compromised Hosts [ESGICH grant to JMA]; Sociedad Andaluza de Trasplante de Órgano Sólido [SATOT grant to LMM]; ESCMID Study Group for Bloodstream Infections and Sepsis (ESGBIS); and ESCMID Study Group for Antimicrobial Resistance Surveillance (ESGARS).

Focal cortical dysplasias (FCDs) are a frequent cause of epilepsy. It has been reported that up to 40% of them cannot be visualized with conventional magnetic resonance imaging (MRI). The main objective of this work was to evaluate by means of a retrospective descriptive observational study whether the automated brain segmentation is useful for detecting rm. One hundred and fifty-five patients, who underwent surgery between the years 2009 and 2016, were reviewed. Twenty patients with FCD confirmed by histology and a preoperative segmentation study, with ages ranging from 3 to 43 years (14 men), were analyzed. Three expert neuroradiologists visually analyzed conventional and advanced MRI with automated segmentation. They were classified into positive and negative concerning visualization of FCD by consensus. Of the 20 patients evaluated with conventional MR1,12 were positive for FCD. Of the negative studies for FCD with conventional MRI, 2 (25%) were positive when they were analyzed with automated segmentation. In 13 of the 20 patients (with positive segmentation for FCD), cortical thickening was observed in 5 (38.5%), while pseudothickening was observed in the rest of patients (8, 61.5%) in the anatomical region of the brain corresponding to the dysplasia. This work demonstrated that automated brain segmentation helps to increase detection of FCDs that are unable to be visualized in conventional MRI images. (C) 2019 Elsevier Inc. All rights reserved.

Treatment of carbapenemase-producing Enterobacterales bloodstream infections in solid organ transplant recipients is challenging. The objective of this study was to develop a specific score to predict mortality in solid organ transplant recipients with carbapenemase-producing Enterobacterales bloodstream infections. A multinational, retrospective (2004-2016) cohort study (INCREMENT-SOT, ClinicalTrials.gov NCT02852902) was performed. The main outcome variable was 30-day all-cause mortality. The INCREMENT-SOT-CPE score was developed using logistic regression. The global cohort included 216 patients. The final logistic regression model included the following variables: INCREMENT-CPE mortality score >= 8 (8 points), no source control (3 points), inappropriate empirical therapy (2 points), cytomegalovirus disease (7 points), lymphopenia (4 points), and the interaction between INCREMENT-CPE score >= 8 and CMV disease (minus 7 points). This score showed an area under the receiver operating characteristic curve of 0.82 (95% confidence interval [CI] 0.76-0.88) and classified patients into 3 strata: 0-7 (low mortality), 8-11 (high mortality), and 12-17 (very-high mortality). We performed a stratified analysis of the effect of monotherapy vs combination therapy among 165 patients who received appropriate therapy. Monotherapy was associated with higher mortality only in the very-high (adjusted hazard ratio [HR] 2.82, 95% CI 1.13-7.06, P = .03) and high (HR 9.93, 95% CI 2.08-47.40, P = .004) mortality risk strata. A score-based algorithm is provided for therapy guidance.

6 páginas, 3 figuras., Fresh chicken meat is a highly perishable product. The effect of immersing chicken breast in an aqueous hop extract (2 g/L) for 18 h at 2°C prior to being vacuum packed, on the growth of spoilage bacteria during a 35-day refrigerated storage period was evaluated in triplicate. The hop extract immersion treatment resulted in a significant decrease (p < 0.05) in lactic acid bacteria counts during the first storage weeks and in Brochothrix thermosphacta counts from day 21 to 35. No effect could be observed on the Enterobacteriaceae growth. A triangle test analysis (54 consumers) tasting hop-extract-immersed vs water-immersed chicken breast showed no differences in cooked chicken flavor. The use of hop extract could increase the shelf life of vacuum-packed fresh chicken without affecting palatability., This work was supported by the CONACYT [MEX/Ref. 288189].

ESMO Virtual Congress -- SEP 19-OCT 18, 2020 -- ELECTR NETWORK WOS:000573469100608 [No abstract available] European Soc Med Oncol AstraZenecaAstraZeneca; MerckMerck & Company AstraZeneca and Merck.

Objective: We set out to analyze the incidence and predictive factors of pulmonary embolism (PE) in hospitalized patients with Covid-19. Methods: We prospectively collected data from all consecutive patients with laboratory-confirmed Covid-19 admitted to the Hospital de la Santa Creu i Sant Pau, a university hospital in Barcelona, between March 9 and April 15, 2020. Patients with suspected PE, according to standardized guidelines, underwent CT pulmonary angiography (CTPA). Results: A total of 1,275 patients with Covid-19 were admitted to hospital. CTPA was performed on 76 inpatients, and a diagnosis of PE was made in 32 (2.6% [95%CI 1.7–3.5%]). Patients with PE were older, and they exhibited lower PaO2:FiO2 ratios and higher levels of D-dimer and C-reactive protein (CRP). They more often required admission to ICU and mechanical ventilation, and they often had longer hospital stays, although in-hospital mortality was no greater than in patients without PE. High CRP and D-dimer levels at admission (=150 mg/L and =1,000 ng/ml, respectively) and a peak D-dimer =6,000 ng/ml during hospital stay were independent factors associated with PE. Prophylactic low molecular weight heparin did not appear to prevent PE. Increased CRP levels correlated with increased D-dimer levels and both correlated with a lower PaO2:FiO2. Conclusions: The 2.6% incidence of PE in Covid-19 hospitalized patients is clearly high. Higher doses of thromboprophylaxis may be required to prevent PE, particularly in patients at increased risk, such as those with high levels of CRP and D-dimer at admission. These findings should be validated in future studies. © Copyright © 2020 Benito, Filella, Mateo, Fortuna, Gutierrez-Alliende, Hernandez, Gimenez, Pomar, Castellvi, Corominas, Casademont and Domingo.

International audience; The queen conch, Strombus (Lobatus) gigas (Linnaeus 1758) is a marine gastropod mollusk. This is an endemic species from the Caribbean Sea, it is present in the water of 37 countries of the Caribbean Sea. It is a valuable marine benthic invertebrate of significant commercial importance in the Caribbean, with an important export trade worth millions of US dollars. The main international markets for conch meat are the USA (79% of the trade) and the French West Indies, with 20%). Also, conch is also consumed locally and its shell and other products sold as curios to visiting tourists in the Caribbean. However, populations have been depleted throughout the Wider Caribbean by overfishing. This species is indexed in Apendix II of CITES, the Convention on International Trade in Endangered Species of Wild Fauna and Flora). The aim of this work was to show results of a scientific collaboration in the Caribbean with various Universities, showing the effect of climatic change on abundance of larvae in this region, in the process of shell calcification and concentration of pollution by microplastics in various sites from the Caribbean.

Shiga toxin-producing Escherichia coli O157: H7 is a food-borne pathogen and the major cause of hemorrhagic colitis. Pseudomonas is the genus most frequent psychrotrophic spoilage microorganisms present in milk. Two-species bacterial systems with Escherichia coli O157: H7, non-pathogenic Escherichia coli, and Pseudomonas fluorescens in skimmed milk at 7, 13, 19, or 25 C was studied. Bacterial interactions were modelled after applying a Bayesian approach. No direct correlation between Pseudomonas fluorescent's growth rate and its effect on the maximum population densities of Escherichia coli species was found. The results show the complexity of the interactions between two species into a food model. The use of natural microbiota members to control foodborne pathogens could be useful to improve food safety during the processing and storage of refrigerated foods., 39 pages, 3 tables, 4 figures, 4 supplement figures

2 páginas, 1 figura.

The queen conch, Strombus (Lobatus) gigas (Linnaeus 1758) is a marine gastropod mollusk. This is an endemic species from the Caribbean Sea, it is present in the water of 37 countries of the Caribbean Sea. It is a valuable marine benthic invertebrate of significant commercial importance in the Caribbean, with an important export trade worth millions of US dollars. The main international markets for conch meat are the USA (79% of the trade) and the French West Indies, with 20%). Also, conch is also consumed locally and its shell and other products sold as curios to visiting tourists in the Caribbean. However, populations have been depleted throughout the Wider Caribbean by overfishing. This species is indexed in Apendix II of CITES, the Convention on International Trade in Endangered Species of Wild Fauna and Flora). The aim of this work was to show results of a scientific collaboration in the Caribbean with various Universities, showing the effect of climatic change on abundance of larvae in this region, in the process of shell calcification and concentration of pollution by microplastics in various sites from the Caribbean.

Diagnosis of von Willebrand disease (VWD) depends on personal and family history of bleeding and confirmatory laboratory testing. Currently available phenotypic tests for VWD contain potential sources for error that may distort results. Despite an exponential growth of information about the von Willebrand factor gene ( VWF ), the role of molecular diagnosis in VWD is still controversial. Due to the complexity and high cost of conventional molecular analyses, some investigators have recommended limiting this approach to distinguish suspected type 2N VWD from hemophilia A, type 2B from platelet-type VWD, and the exploration of type 3 VWD. New genetic methodologies and approaches are becoming available, but there is still some reluctance for their implementation in VWD diagnosis. This article discusses the pros and cons of molecular testing in VWD considering the experience obtained through the multicenter project "Molecular and Clinical Profile of VWD in Spain (PCM-EVW-ES)."

Diagnosis of von Willebrand disease (VWD) depends on personal and family history of bleeding and confirmatory laboratory testing. Currently available phenotypic tests for VWD contain potential sources for error that may distort results. Despite an exponential growth of information about the von Willebrand factor gene ( VWF ), the role of molecular diagnosis in VWD is still controversial. Due to the complexity and high cost of conventional molecular analyses, some investigators have recommended limiting this approach to distinguish suspected type 2N VWD from hemophilia A, type 2B from platelet-type VWD, and the exploration of type 3 VWD. New genetic methodologies and approaches are becoming available, but there is still some reluctance for their implementation in VWD diagnosis. This article discusses the pros and cons of molecular testing in VWD considering the experience obtained through the multicenter project "Molecular and Clinical Profile of VWD in Spain (PCM-EVW-ES)."

3 páginas, 3 tablas.- Trabajo presentado al: XVIII Jornadas sobre Producción Animal AIDA. Zaragoza, España, 7-8 mayo 2019., Proyecto CSI042-P17 Junta de Castilla y León. Cristina Saro disfrutó de un contrato postdoctoral financiado por el Fondo Europeo de Desarrollo Regional.

Trabajo presentado al X Congreso Nacional CYTA/CESIA. León, España, 15-17 mayo, 2019., Se compararon las características de la canal y de la carne de corderos pesados de raza assaf con diferente eficiencia alimentaria, parámetro relacionado con la cantidad de alimento que consume cada cordero para alcanzar una determinada ganancia de peso. Los resultados obtenidos sugieren que, en las condiciones del presente estudio, la eficiencia con que los animales utilizan el mismo alimento no guarda relación ni con la composición de la canal ni de la carne, pero sí influye negativamente en el rendimiento comercial., Proyecto CSI047 P17 de la Junta de Castilla y León. Cristina Saro disfrutó de un contrato posdoctoral financiado por el Fondo Europeo de Desarrollo Regional.

Purpose : The association between mitochondrial DNA (mtDNA) haplogroup and the risk of neovascular age-related macular degeneration (nAMD) has been reported. This prospective, observational clinical study analyzes the anatomical and functional outcomes after one year of intravitreal ranibizumab therapy in nAMD patients according to the mt-DNA haplogroup. Methods : Ninety-eight eyes corresponding to 91 patients with nAMD were treated with three consecutive monthly intravitreal ranibizumab 0.5 mg followed by a flexible pro re nata (PRN). Retreatment criteria were: loss =5 ETDRS letters; increased central foveal thickness (CFT) >100 µm; intra or subretinal fluid on OCT; new or persistent macular haemorrhages. Exclusion criteria were previous treatment with photodynamic therapy or anti-VEGF drug; vitrectomy or any other retinal disease; corneal or lens opacities. Patients were classified into four groups according to their mtDNA haplogroup determined by PCR (Hg H- m.7028C>T; Hg HV- m.14766T>C; Hg JT- m.421 6T>C and Hg U- m.12308A>G). Chi-square, Kolmogorov-Smirnov, ANOVA, Mann-Whitney, Kruskal-Wallis, analysis of variance, Wilcoxon, and Friedman tests were used. SPSS 21.0 program. A p value < 0.05 was statistically significant. Results : Gender, age, risk factors (smoking, diabetes, arterial hypertension and dyslipidemia) and neovascular lesion type were equally distributed among four groups. There were no significant differences between groups in baseline or after loading fase in best corrected visual acuity (BCVA) or CFT. At 4 months, BCVA was significantly higher than baseline in haplogroups HV (p = 0.02), JT (p = 0.002) and U (p = 0.002). CFT was significantly lower in all haplogroups (HV, p

Trabajo presentado al 2nd. Food Chemistry Conference. Sevilla, España, 17-19 septiembre, 2019., Fresh sausages are commonly packaged in anaerobic modified atmospheres and stored under refrigeration in order to achieve a long shelf life, i.e. few weeks. During storage and due to the internal conditions of fresh sausages (pH, aw, nutrient availability) there is a progressive development of facultative anaerobic bacteria, i.e. lactic acid bacteria or enterobacteria, which in the course of time results in product spoilage. The main reason for this is the production volatile compounds from microbial metabolism which can lead on the generation of undesirable flavours. In this study, the volatile compounds in a fresh Bosnian-style lamb sausage during refrigerated storage under 20% CO2 and 80% N2 atmosphere were weekly monitored for 35 days. Sausages were prepared in triplicate and volatiles were determined by head space solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry. The microbial origin volatiles detected at day 0 (stuffing into casings) were diacetyl, hexanol and ethylhexanol. At day 7 of storage appeared 3-methyl-butanal, 3-methyl-butanol and acetoin, at day 14 ethyl acetate, ethylhexanoate and benzene acetaldehyde, and at day 28 butanediol. Most of them tended to increase during storage until a point where their concentrations stabilise. Relevant increases were observed for diacetyl from day 0 to 7, 1-hexanol from day 14 to 21 (then tended to decrease) and acetoin from day 21 to 28. Moreover, ethyl acetate, acetoin, 3-methyl-butanol, ethylhexanoate and ethylhexanol increased from the day where they were detected to day 28. Meanwhile, butanediol and benzene acetaldehyde increased until the end of storage. Volatiles presumably originated from lipid oxidation, such as hexanal, 1-octen-3-ol or heptanal, were also detected and their concentration tended to be constant or decrease with time. Changes in volatiles due to microbial metabolism were already observed at day 7 of storage; however, the major changes were observed from days 14 to 28.

3 páginas, 3 tablas.- Trabajo presentado al: XVIII Jornadas sobre Producción Animal AIDA. Zaragoza, España, 7-8 mayo 2019. Proyecto CSI042-P17 Junta de Castilla y León. Cristina Saro disfrutó de un contrato postdoctoral financiado por el Fondo Europeo de Desarrollo Regional.

Molluscs are subjected to intense harvesting in many areas around the world. Conservation measures have been developed to preserve populations of an overexploited gastropod species, the abalone Haliotis tuberculata coccinea. This species was surveyed in subtidal localities throughout coastal Tenerife, Canary Islands over the last two decades (1994–2014). A clear indicator of non-recovery was observed in the decrease of mean size throughout the last two decades, even after the inclusion of this species in the Regional Catalogue of Endangered Species. The mean size of abalones decreased from 33.5 mm (1994) to 28–29 mm (2002 and 2014), corresponding to sub-adult individuals. The structure of size classes was typical of an overexploited species, with reduced occurrence of large individuals (>50 mm). Several factors might explain this pattern such as illegal harvesting, proliferation of featureless benthos and a decrease of suitable habitats for colonization and settlement. Complementary conservation actions are urgently needed to preserve this species in the area studied.

Contains fulltext : 171291.pdf (Publisher’s version ) (Open Access) BACKGROUND: The availability of multiple new treatments for metastatic castration-resistant prostate cancer (mCRPC) mandates earlier treatment switches in the absence of a response. A decline in prostate-specific antigen (PSA) is widely used to monitor treatment response, but is not validated as an intermediate endpoint for overall survival (OS). OBJECTIVE: To evaluate the association between early PSA decline and OS following abiraterone acetate (AA) treatment. DESIGN, SETTING, AND PARTICIPANTS: We identified mCRPC patients treated with AA before or after docetaxel at the Royal Marsden NHS Foundation Trust between 2006 and 2014. Early PSA decline was defined as a 30% decrease in PSA at 4 wk relative to baseline, and early PSA rise as a 25% increase. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Association with OS was analyzed using multivariate Cox regression and log-rank analyses. Spearman's rho correlation coefficient (r) was calculated to evaluate the association between PSA changes at 4 wk and 12 wk. RESULTS AND LIMITATIONS: There were 274 patients eligible for this analysis. A 30% PSA decline at 4 wk was associated with longer OS (25.8 vs 15.1 mo; hazard ratio [HR] 0.47, p

The global sustainability indices most used by companies (FTSE4Good, CDP, MSCI, Goldman Sachs, SUSTAINALYTICS, DJSI) dedicate part of their analysis to corporate governance aspects, as the correlation between the governance dimension and the success of sustainability has been empirical demonstrated.Despite this unanimity on including governance aspects, the indices do not share a common methodology, questionnaire or objectives. It is, therefore, difficult to obtain an overall vision of what aspects relating to corporate governance have greater impact on sustainability.The opportunity for research lies in conducting a critical review of the perspective that sustainability indices currently offer on the corporate governance dimension in order to find out, due to the absence of a common methodology, if questions are being asked on all of the variables that should be considered, or if some are being omitted. Los índices globales de sostenibilidad más utilizados por las empresas (FTSE4Good, CDP, MSCI, Goldman Sachs, SUSTAINALYTICS, DJSI), dedican una parte de su análisis a los aspectos del gobierno corporativo, ya que se ha demostrado empíricamente la existencia de una correlación entre la dimensión del gobierno y el éxito de la sostenibilidad.Sin embargo, a pesar de esta unanimidad en incluir los aspectos de gobierno, los índices no comparten ni objetivos, ni metodología, ni un cuestionario común, por lo que es complejo tener una visión global sobre qué aspectos vinculados al gobierno corporativo tienen mayor impacto en la sostenibilidad.La oportunidad de la investigación radica en hacer una revisión crítica sobre el tratamiento que ofrecen actualmente los índices de sostenibilidad a la dimensión gobierno corporativo, para conocer si, como consecuencia de la ausencia de una metodología común, se está preguntando por todas las variables que se deberían tener en cuenta, o están obviando algunas.

This paper continues the taxonomic revision of the species collected during the campaign of 2015 in the National Park of Ordesa and Monte Perdido. 409 taxa are added to the previous inventory, some of them from alpine-subalpine ecology, of which 76% correspond to phylum Basidiomycota and 22.2% to Ascomycota. They are presented in the form of a check-list, followed by 20 selected taxonomic descriptions of interesting, infrequent species, and those though to be new in the peninsular territory. Among the species determined, six species were included in the proposals for the inventory of protected species of the Iberian Peninsula and/or Aragon. This paper presents a first approximation, as a platform for later evaluations, of the beech conservation degree in some forests from the Park through the occurrence of indicator species. En este trabajo se continúa con la revisión taxonómica de las especies recolectadas durante la campaña de 2015 en el Parque Nacional de Ordesa y Monte Perdido. Se añaden al catálogo previo 409 taxones, algunos de ellos de ecología alpina-subalpina, de los cuales el 76% corresponde al phylum Basidiomycota y el 22,2% al Ascomycota. Se presentan en forma de listado, y a continuación una selección de 20 descripciones taxonómicas completas, que corresponden con especies interesantes, poco frecuentes y posiblemente nuevas en el territorio peninsular. De las especies estudiadas se han encontrado seis que han sido propuestas para el inventario de especies protegidas de la península ibérica y/o Aragón. Se presenta en este trabajo una primera aproximación, a modo de punta de lanza para evaluaciones posteriores, del grado de conservación de algunos hayedos del Parque a través de la ocurrencia de especies indicadoras.

CONTEXT: For more precise, personalized care in prostate cancer (PC), a new classification based on molecular features relevant for prognostication and treatment stratification is needed. Genomic aberrations in the DNA damage repair pathway are common in PC, particularly in late-stage disease, and may be relevant for treatment stratification. OBJECTIVE: To review current knowledge on the prevalence and clinical significance of aberrations in DNA repair genes in PC, particularly in metastatic disease. EVIDENCE ACQUISITION: A literature search up to July 2016 was conducted, including clinical trials and preclinical basic research studies. Keywords included DNA repair, BRCA, ATM, CRPC, prostate cancer, PARP, platinum, predictive biomarkers, and hereditary cancer. EVIDENCE SYNTHESIS: We review how the DNA repair pathway is relevant to prostate carcinogenesis and progression. Data on how this may be relevant to hereditary cancer and genetic counseling are included, as well as data from clinical trials of PARP inhibitors and platinum therapeutics in PC. CONCLUSIONS: Relevant studies have identified genomic defects in DNA repair in PCs in 20-30% of advanced castration-resistant PC cases, a proportion of which are germline aberrations and heritable. Phase 1/2 clinical trial data, and other supporting clinical data, support the development of PARP inhibitors and DNA-damaging agents in this molecularly defined subgroup of PC following success in other cancer types. These studies may be an opportunity to improve patient care with personalized therapeutic strategies. PATIENT SUMMARY: Key literature on how genomic defects in the DNA damage repair pathway are relevant for prostate cancer biology and clinical management is reviewed. Potential implications for future changes in patient care are discussed.

Micro-Abstract We retrospectively evaluated the effect of locoregional treatment (LRT) on overall survival (OS) in 300 metastatic at diagnosis (M1) prostate cancer patients. LRT was associated in univariate and multivariate analysis with longer OS, which remained significant for radiotherapy but not for transurethral prostatectomy. These data support further prospective evaluation of the benefit of local control in this patient population., Background The optimal management of the primary tumor in metastatic at diagnosis (M1) prostate cancer (PCa) patients is not yet established. We retrospectively evaluated the effect of locoregional treatment (LRT) on overall survival (OS) hypothesizing that this could improve outcome through better local disease control and the induction of an antitumor immune response (abscopal effect). Patients and Methods M1 at diagnosis PCa patients referred to the Prostate Targeted Therapy Group at the Royal Marsden between June 2003 and December 2013 were identified. LRT was defined as either surgery, radiotherapy (RT) or transurethral prostatectomy (TURP) administered to the primary tumor at any time point from diagnosis to death. Kaplan–Meier analyses generated OS data. The association between LRT and OS was evaluated in univariate (UV) and multivariate (MV) Cox regression models. Results Overall 300 patients were identified; 192 patients (64%) experienced local symptoms at some point during their disease course; 72 patients received LRT (56.9% TURP, 52.7% RT). None of the patients were treated with prostatectomy. LRT was more frequently performed in patients with low volume disease (35.4% vs. 16.2%; P < .001), lower prostate-specific antigen (PSA) level at diagnosis (median PSA: 75 vs. 184 ng/mL; P = .005) and local symptoms (34.2% vs. 4.8%; P < .001). LRT was associated in UV and MV analysis with longer OS (62.1 vs. 55.8 months; hazard ratio [HR], 0.74; P = .044), which remained significant for RT (69.4 vs. 55.1 months; HR, 0.54; P = .002) but not for TURP. RT was associated with better OS independent of disease volume at diagnosis. Conclusion These data support the conduct of randomized phase III trials to evaluate the benefit of local control in patients with M1 disease at diagnosis.

Despite their relatively low prevalence, vascular diseases of the liver represent a significant health problem in the field of liver disease. A common characteristic shared by many such diseases is their propensity to cause portal hypertension together with increased morbidity and mortality. These diseases are often diagnosed in young patients and their delayed diagnosis and/or inappropriate treatment can greatly reduce life expectancy. This article reviews the current body of evidence concerning Budd-Chiari syndrome, non-cirrhotic portal vein thrombosis, idiopathic portal hypertension, sinusoidal obstruction syndrome, hepatic vascular malformations in hereditary haemorrhagic telangiectasia, cirrhotic portal vein thrombosis and other rarer vascular diseases including arterioportal fistulas. It also includes a section on the diagnostic imaging of vascular diseases of the liver and their treatment from a haematological standpoint (study of thrombotic diathesis and anticoagulation therapy). All recommendations are based on published studies extracted from PubMed. The quality of evidence and strength of recommendations were rated in accordance with the GRADE system (Grading of Recommendations, Assessment Development and Evaluation). In the absence of sufficient evidence, recommendations were based on the opinion of the committee that produced the guide., Las enfermedades vasculares hepáticas, a pesar de su relativamente baja prevalencia, representan un problema de salud importante en el campo de las enfermedades hepáticas. Una característica común a muchas de estas enfermedades es que pueden causar hipertensión portal, con la elevada morbimortalidad que ello conlleva. Con frecuencia estas enfermedades se diagnostican en pacientes jóvenes y el retraso en su diagnóstico y/o un tratamiento inadecuado pueden reducir de forma importante la esperanza de vida. El presente artículo revisa la evidencia actual en el síndrome de Budd-Chiari, la trombosis venosa portal en pacientes no cirróticos, la hipertensión portal idiopática, el síndrome de obstrucción sinusoidal, las malformaciones vasculares hepáticas en la telangiectasia hemorrágica hereditaria, la trombosis portal en la cirrosis, otras patologías vasculares menos frecuentes como las fístulas arterioportales, así como un apartado sobre el diagnóstico por imagen de las enfermedades vasculares hepáticas y su tratamiento desde el punto de vista hematológico (estudio de la diátesis trombótica y tratamiento anticoagulante). Las recomendaciones se han realizado de acuerdo a los estudios publicados extraídos de Pubmed. La calidad de la evidencia y la intensidad de las recomendaciones fueron graduadas de acuerdo al sistema Grading of Recommendations Assessment Development and Evaluation (GRADE). Cuando no existían evidencias suficientes, las recomendaciones se basaron en la opinión del comité que redactó la guía.

Trabajo presentado al 8th International UHMWPE Meeting, celebrado en Torino (Italia) del 19 al 20 de octubre de 2017., DurationTM Stabilized Polyethylene (Stryker Howmedica) has been the precursor of modern highly crosslinked polyethylenes in total hip joint arthroplasty. DurationTM Polyethylene was subjected to gamma sterilization in low-oxygen blisters and post-irradiation annealing to promote crosslinking and free radical recombination. Thus, DurationTM components were anticipated to exhibit enhanced oxidative stability and increased crosslink density -and therefore superior wear resistance- than historical, gamma-irradiated in air, components. Previous studies have reported lower in vitro and radiographic wear rates for DurationTM acetabular inserts. However, it is unclear whether the wear reduction, with its potential benefit to bone stock, and the oxidative stability of DurationTM are maintained after long-term implantation. Our objective was to assess the clinical, wear and oxidative performance of DurationTM polyethylene acetabular inserts retrieved after long-term implantation., This study was funded by University of Zaragoza (Project JIUZ-2014-TEC-10) and Diputación General de Aragón (Project T40-Grupo Consolidado de Investigación en Biomateriales).

5 páginas, 3 tablas.--Trabajo presentado a las: XLI Jornadas Científicas y XVII Jornadas Internacionales de Ovinotecnia y Caprinotecnia. (Talavera de la Reina, Toledo, España, 14-16 septiembre, 2016)., Proyecto Intramural Especial del CSIC (201540E084). Carolina Blanco es beneficiaria de un contrato predoctoral de la Junta de Castilla y León y el Fondo Social Europeo

12 páginas, 6 tablas., Thirty-two lambs were used to study the effect of sunflower soap stocks (SS), a by-product from the vegetable oil refinery industry, on meat chemical composition, fatty acid profile, volatile compounds, and consumer acceptability. Lambs were finished (average length of fattening period 35 ± 7.3 d, 26.8 ± 0.09 kg final BW) on a pelleted total mixed ration (TMR) with no SS (00SS) or including 15, 30 or 60 g SS/kg (15SS, 30SS, and 60SS, respectively). Sunflower soap stocks decreased the percentage of SFA, increased the proportion of trans-MUFA (P < 0.05), and modified the levels of several odor-active lipid-derived volatile compounds (P < 0.05). Consumers were able to distinguish between control and 15SS meat samples in a triangular test (P < 0.05), but a well-defined preference for meat of any of these treatments was not exhibited (P > 0.05). Atherogenicity and saturation indexes decreased by 31% and 27%, respectively, in SS groups compared to control (linear P < 0.05). However, certain volatile compounds (benzene and toluene) and 10t-18:1 fatty acid, known potential hazards for human health, were increased in meat from lambs fed TMR with SS. For this reason, only inclusion rates up to 15 g SS/kg TMR seem to sustain a satisfactory balance between beneficial and detrimental effects on lamb meat composition and quality., Financial support was received from the Spanish Ministry of Science and Innovation, Project AGL2010-19094. L. Morán was supported by a “JAE-Predoc” grant contract under the program “Junta para la Ampliación de Estudios” (CSIC-European Social Fund); R. Bodas had a subprogram “Doc-INIA” contract, and C. Blanco is supported by a contract for young researchers (Council of Castille and Leon and European Social Fund). L. H. Villalobos was supported by the “Programa para el Mejoramiento del Profesorado” (PROMED, México).

The behavior of a downstream fixed-bed of porous particles (a mixture of activated alumina and charcoal) was investigated as a low-cost alternative for upgrading the pyrolysis gas released from a biochar production process (slow pyrolysis in a fixed-bed reactor). Results showed that both pressure and bed composition (in terms of charcoal mass fraction) had significant effects on the yield of producer gas, as well as on its composition. Both porous materials exhibited a relatively good performance in terms of tar removal. The best results (highest yield of gas and maximum H2 content) were obtained when the fixed-bed was composed entirely of charcoal and the absolute pressure was increased from 0.1 to 1.0 MPa., Proceedings of the 22nd European Biomass Conference and Exhibition, 23-26 June 2014, Hamburg, Germany, pp. 1155-1159

Background-Genetics plays an important role in venous thromboembolism (VTE). Factor V Leiden (FVL or rs6025) and prothrombin gene G20210A (PT or rs1799963) are the genetic variants currently tested for VTE risk assessment. We hypothesized that primary VTE risk assessment can be improved by using genetic risk scores with more genetic markers than just FVL-rs6025 and prothrombin gene PT-rs1799963. To this end, we have designed a new genetic risk score called Thrombo inCode (TiC). Methods and Results-TiC was evaluated in terms of discrimination ( of the area under the receiver operating characteristic curve) and reclassification (integrated discrimination improvement and net reclassification improvement). This evaluation was performed using 2 age- and sex-matched case-control populations: SANTPAU (248 cases, 249 controls) and the Marseille Thrombosis Association study (MARTHA; 477 cases, 477 controls). TiC was compared with other literature-based genetic risk scores. TiC including F5 rs6025/rs118203906/rs118203905, F2 rs1799963, F12 rs1801020, F13 rs5985, SERPINC1 rs121909548, and SERPINA10 rs2232698 plus the A1 blood group (rs8176719, rs7853989, rs8176743, rs8176750) improved the area under the curve compared with a model based only on F5-rs6025 and F2-rs1799963 in SANTPAU (0.677 versus 0.575, P

The sea urchin Diadema antillarum was the most important herbivore on Caribbean reefs until 1983, when mass mortality reduced its populations by more than 97%. Knowledge of its past demography is essential to reconstruct reef ecology as it was before human impact, which has been implicated as having caused high pre–mortality Diadema abundance. To determine the history of its population size, we sequenced the ATPase 6 and 8 region of mitochondrial DNA from populations in the Caribbean and in the eastern Atlantic (which was not affected by the mass mortality), as well as from the eastern Pacific D. mexicanum . The Caribbean population harbours an order of magnitude more molecular diversity than those of the eastern Pacific or the eastern Atlantic and, despite the recent mass mortality, its DNA sequences bear the genetic signature of a previous population expansion. By estimating mutation rates from divergence between D. antillarum and D. mexicanum , that were separated at a known time by the Isthmus of Panama, and by using estimates of effective population size derived from mismatch distributions and a maximum likelihood coalescence algorithm, we date the expansion as having occurred no more recently than 100 000 years before the present. Thus, Diadema was abundant in the Caribbean long before humans could have affected ecological processes; the genetic data contain no evidence of a recent, anthropogenically caused, population increase.

3 páginas, 1 tabla, 1 figura.-- Trabajo presentado a las XV Jornadas sobre Producción Animal AIDA (Zaragoza, 14 al 15 de mayo, 2013)., Assaf suckling lambs (32) fed milk replacer (MR) ad libitum were supplemented with: no additives (CTRL group n=8), carnosic acid at 0,096 g kg-1 live weight (LW) (CARN group n= 8), or vitamin E at 0.024 g kg-1 LW (VITE group n=8) were used to assess the effect of these antioxidant compounds on oxidation stability. The animals were slaughtered at reached intended body weight and gluteus medius muscles were extracted for thiobarbituric acid reactive substances (TBARs), cholesterol oxidation products or oxysterols (OXs) determination, sliced, packed and kept at 4 ºC for 14 days. Results indicated that the VITE group showed significatly lower levels of malondialdehide (at day 14 of storage) and the 7 - hydroxycholesterol production when compared to the CTRL group, showing CARN group intermediate levels., al Ministerio de Economía y Competitividad y Consejo Superior de Investigaciones Científicas por la financiación aportada (Proyecto 201240E105) y al programa del CSIC Junta para la Ampliación de Estudios por la concesión de la Beca JAEPredoc a Lara Morán.