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The main goal of this study was to examine some of the underlying mechanisms of protection by ferulic acid (FA) from damage caused by free radicals, mainly mechanisms involving enzymes to suppress formation of free radicals and the sequestration of transition metal ions that catalyse free radical production. Hence, potential of FA to act as antioxidant via suppressing •OH radical formation by sequestering the catalytic Fe2+ ion, and by inhibiting prooxidative lipoxygenase (LOX) enzyme, i.e., by suppressing formation of free radical precursors, was studied. The M06-2X functional and 6-311++G(d, p) basis set implemented in the Gaussian 09 program package [1] was used for geometry optimisations and frequency calculations. All calculations were performed in gas-phase at 298.15 K. The AutoDock 4.0 software package [2] was used to predict the binding mode between LOX and investigated compounds, FA and 5- hydroxyferulic acid (5-OHFA). The crystal structure of LOX was taken from Protein Data Bank. Discovery Studio 4.0 [3] was used to prepare protein structures and for analysis of the obtained results. Polar hydrogen atoms were added using the hydrogen module in AutoDockTools (ADT) graphical interface. Grid maps were determined using the AutoGrid module with grid box of dimension 60×60×60 with point separated by 0.375 Å. The Lamarckian Genetic Algorithm (LGA) was implemented for rigid-flexible docking simulation. Results of molecular docking calculations indicate favourable binding interactions of FA and 5OHFA with LOX active site. Exergonicity of chelation reactions of catalytic Fe2+ ion with FA and 5OHFA indicate potency of these chelators to prevent the formation of •OH radicals via Fenton-like reactions. Based on obtained results, inhibition of LOX could be more relevant mechanisms of protection against oxidative stress than iron chelation.