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82 results on '"Martano, Manuela"'

7. Microwave Radiation for Plant Protection: the Red Palm Weevil Pest Control Case Study

Author

MASSA RITA, MIGLIORE MD, PANARIELLO G, PINCHERA D, SCHETTINO F, CAPRIO EMILIO, MARTANO MANUELA, MAIOLINO PAOLA, PAGANO IA, GRIFFO RAFFAELE, IV CONV NAZIONALE ICEMB (INTERAZIONI CAMPI ELETTROMAGNETICI E SISTEMI BIOLOGICI), Massa, Rita, Migliore, Md, Panariello, G, Pinchera, D, Schettino, F, Caprio, Emilio, Martano, Manuela, Maiolino, Paola, Pagano, Ia, and Griffo, Raffaele

Published
2016

10. Histopathology as diagnostic tool for honey bee diseases

Author

MARTANO, MANUELA, RESTUCCI, BRUNELLA, CRINGOLI, GIUSEPPE, RINALDI, LAURA, MAIOLINO, PAOLA, Iafigliola L, De Leva G, Società Italiana delle Scienze Veterinarie, Martano, Manuela, Restucci, Brunella, Iafigliola, L, Cringoli, Giuseppe, De Leva, G, Rinaldi, Laura, and Maiolino, Paola

Published
2013

14. PILOMATRICOMA MALIGNO CON METASTASI OSSEA IN UN CANE

Author

MARTANO, MANUELA, NAVAS, LUIGI, MAIOLINO, PAOLA, Falleti J., Ilsami R., Meomartino L.e., Martano, Manuela, Navas, Luigi, Falleti, J., Ilsami, R., Meomartino, L. e., and Maiolino, Paola

Published
2010

17. STUDIO ISTOPATOLOGICO SUL MUSCOLO DI CANI ANZIANI

Author

Costagliola A., Pagano T. B., TRAPANI, FRANCESCA, MARTANO, MANUELA, PAPPARELLA, SERENELLA, PACIELLO, ORLANDO, g. De Vico, Costagliola, A., Pagano, T. B., Trapani, Francesca, Martano, Manuela, Papparella, Serenella, and Paciello, Orlando

Subjects
sarcopenia, muscolo, cane
Published
2009

21. Miopatie congenite nel puledro

Author

PACIELLO, ORLANDO, PASOLINI, MARIA PIA, MARTANO, MANUELA, PAPPARELLA, SERENELLA, F. Trapani, Atti II Meeting AIPVet, Paciello, Orlando, Pasolini, MARIA PIA, Martano, Manuela, F., Trapani, and Papparella, Serenella

Published
2005

24. Vascular tumours associated with Dirofilaria repens infestion in a dog

Author

MARTANO, MANUELA, VENEZIANO, VINCENZO, PACIELLO, ORLANDO, RUSSO, VALERIA, MAIOLINO, PAOLA, M. Santaniello, S. Carbone, M. Cataldi, Martano, Manuela, Veneziano, Vincenzo, M., Santaniello, S., Carbone, Paciello, Orlando, M., Cataldi, Russo, Valeria, and Maiolino, Paola

Published
2004

36. Evaluation of angiogenesis in canine mammary tumours

Author

RESTUCCI B, PAPPARELLA, SERENELLA, MAIOLINO, PAOLA, MARTANO, MANUELA, DE VICO, GIONATA, Restucci, B, Maiolino, Paola, Martano, Manuela, DE VICO, Gionata, and Papparella, Serenella

Published
2002

37. Indicatori biologici di inquinamento ambientale

Author

PACIELLO, ORLANDO, RESTUCCI B, MAIOLINO P. E. PAPPARELLA S., MARTANO, MANUELA, Paciello, Orlando, Martano, Manuela, Restucci, B, and Maiolino, P. E. PAPPARELLA S.

Published
2002

38. Expression of beta-catenin, E-cadherin and APC in canine mammary tumors

Author

Restucci, Brunella, Maiolino, Paola, Martano, Manuela, Esposito, Giuseppe, Filippis, Daniele, Borzacchiello, Giuseppe, Lorenzo Lo Muzio, Restucci, Brunella, Maiolino, Paola, Martano, Manuela, Esposito, G, De Filippis, D, Borzacchiello, Giuseppe, and Lo Muzio, L.

Subjects
Microscopy, Confocal, adhesion molecules, breast, carcinogenesis, dog, catenin, cadherin, APC, Adenomatous Polyposis Coli Protein, Blotting, Western, Fluorescent Antibody Technique, Mammary Neoplasms, Animal, Adenocarcinoma, Cadherins, Dogs, Animals, Dog Diseases, beta Catenin
Abstract

Mammary tumors are a very common neoplasm in the dog and show histological features and biological behaviour similar to human mammary carcinomas. Recently, a pathway named Wnt-1, involving beta-catenin and APC protein, has emerged as an important player in many human tumor types, including mammary neoplasms.Thirty-five samples of canine mammary tumors (10 benign and 25 malignant) were studied in order to evaluate the co-expression of beta-catenin, APC protein and E cadherin with confocal laser microscopical observation, by western blot analysis and by correlating data obtained with the histological grade of tumours.A progressive decrease of E-cadherin together with disruption of beta-catenin expression was observed in less differentiated malignant tumors. In addition, a loss of beta-catenin membranous distribution and a cytoplasmic accumulation was often coexpressed with disrupted expression of APC protein. Western blot analysis showed a progressive increase of beta-catenin in malignant tumors, which could be the expression of disrupted /-catenin catabolism leading to cytoplasmic accumulation. In some less differentiated malignant tumors, a marked beta-catenin decrease was also observed. This feature could be linked to mutations in beta-catenin gene coding for a truncated and lighter protein.These results may indicate the multifunctional role played by beta-catenin in canine mammary oncogenesis.

39. Microwaves Induce Histological Alteration of Ovaries and Testis in Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae)

Author

Manuela Martano, Rita Massa, Brunella Restucci, Emilio Caprio, Raffaele Griffo, Karen Power, Paola Maiolino, Martano, Manuela, Massa, Rita, Restucci, Brunella, Caprio, Emilio, Griffo, Raffaele, Power, Karen, and Maiolino, Paola

Subjects
histology, Keywords: RPW, microwave, RPW, Agronomy and Crop Science, microwaves, reproductive system
Abstract

The Red Palm Weevil (RPW) is one of the major pests of palms, frequently leading to the plants death. Action plans and the development of bio/physical strategies to contrast RPW diffusions are strongly recommended due to the serious concerns related to environmental pollution and insects’ resistance to chemicals. In the present study, we investigated morphological alterations of the ovaries and testes in adult RPW exposed to 2.45 GHz for 5, 15, and 30 s. During these treatments, the relative increase in temperature and the days of survival after irradiation were monitored. Then, RPWs were processed for macroscopical and microscopical analysis. Histological lesions of the ovaries and testes were characterized by the degeneration and necrosis of germinal cells, which increased with the increase in the time of irradiation and the temperature. By the same token, an increase in the temperature of irradiated insects was associated with a decrease in their survival time. These observations lead us to conclude that MWs could represent a useful tool for reducing or eliminating the reproductive capacity of this dreaded insect.

Published
2023
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40. Prevalence of Eucoleus garfiai in Wild Boars Hunted at Different Altitudes in the Campania and Latium Regions (Italy)

Author

Karen Power, Manuela Martano, Nadia Piscopo, Paolo Viola, Gennaro Altamura, Vincenzo Veneziano, Ana Carvajal Urueña, Luigi Esposito, Power, Karen, Martano, Manuela, Piscopo, Nadia, Viola, Paolo, Altamura, Gennaro, Veneziano, Vincenzo, Carvajal Urueña, Ana, and Esposito, Luigi

Subjects
General Veterinary, nematode, histopathology, bioindicator, Animal Science and Zoology, soil quality, wild boar
Abstract

Recent reports of Eucoleus garfiai in wild boars in southern Italy have highlighted the need for collecting epidemiological data on the presence of this parasite and understanding the role of possible interactions between wild boars, E. garfiai, and the environment. This study analyses, using histopathological and biomolecular techniques, the presence of E. garfiai in tongue samples of wild boars hunted in four provinces of the Campania and Latium regions (Italy), in areas located above and below 900 m above sea level (asl). Histopathological examinations revealed the presence of adults and eggs of nematodes, which were subsequently identified as E. garfiai by biomolecular analysis, in the tongue epithelium. The detection of the parasite was more frequent in samples collected from hunting areas located above 900 m asl than in those collected from areas located below 900 m asl (66.67% vs. 38.09%; p < 0.01). Some species of earthworms are intermediate hosts of E. garfiai and it is well known that earthworms are more present in high quality soils. Therefore, we can suggest that the higher prevalence of E. garfiai at higher altitudes is probably linked to a greater presence of earthworms in the soil, due to its higher quality in these areas.

Published
2023

41. Beclin 1, LC3 and P62 Expression in Equine Sarcoids

Author

Manuela Martano, Gennaro Altamura, Karen Power, Pierluigi Liguori, Brunella Restucci, Giuseppe Borzacchiello, Paola Maiolino, Martano, Manuela, Altamura, Gennaro, Power, Karen, Liguori, Pierluigi, Restucci, Brunella, Borzacchiello, Giuseppe, and Maiolino, Paola

Subjects
autophagy, General Veterinary, QL1-991, Veterinary medicine, SF600-1100, equine sarcoid, Animal Science and Zoology, equine sarcoids, BPV, Zoology, Article
Abstract

Simple Summary Equine sarcoids, caused by bovine papillomaviruses, are equine skin tumors of fibroblastic origin. It is well known that bovine papillomaviruses are able to interfere with the survival and proliferation of cells by regulating autophagy, a mechanism implicated in the breakdown and reuse of old and damaged cellular material. The present study focused on the evaluation in equine sarcoids and normal skins of the expression level of some of the main proteins involved in the autophagic pathway, such as Beclin 1, LC3 and P62, by immunohistochemical and biochemical techniques. Results obtained in equine sarcoids suggested an alteration of the autophagic process which could lead to a predominance of a particular population of fibroblast. Those fibroblasts could survive longer in a hypoxic microenvironment and produce more and/or altered collagen, giving an origin to the equine sarcoid. Abstract Background: It is well known that δ-bovine papillomaviruses (BPV-1, BPV-2 and BPV-13) are one of the major causative agents of equine sarcoids, the most common equine skin tumors. Different viruses, including papillomaviruses, evolved ingenious strategies to modulate autophagy, a complex process involved in degradation and recycling of old and damaged material. Methods: The aim of this study was to evaluate, by immunohistochemistry (IHC) and Western blot (WB) analysis, the expression of the main related autophagy proteins (Beclin 1, protein light chain 3 (LC3) and P62), in 35 BPV1/2 positive equine sarcoids and 5 BPV negative normal skin samples. Results: Sarcoid samples showed from strong-to-moderate cytoplasmic immunostaining, respectively, for Beclin 1 and P62 in >60% of neoplastic fibroblasts, while LC3 immunostaining was weak to moderate in ≤60% of neoplastic fibroblasts. Western blot analysis confirmed the specificity of the antibodies and revealed no activation of autophagic flux despite Beclin 1 overexpression in sarcoid samples. Conclusion: Results could suggest the activation of the initial phase of autophagy in equine sarcoids, and its impairment during the following steps. The impairment of autophagy could lead to a selection of a quiescent population of fibroblasts, which survive longer in a hypoxic microenvironment and produced more and/or altered collagen.

Published
2022

42. Detection of Honeybee Viruses in Vespa orientalis

Author

Karen Power, Gennaro Altamura, Manuela Martano, Paola Maiolino, Power, Karen, Altamura, Gennaro, Martano, Manuela, and Maiolino, Paola

Subjects
Microbiology (medical), Infectious Diseases, Immunology, Microbiology
Abstract

The Oriental hornet (Vespa orientalis) is spreading across the Italian territory threatening the health and wellbeing of honeybees by feeding on adult individuals and larvae and by plundering hive resources. Considering the capacity of other hornets in harboring honeybee viruses, the aim of this study was to identify the possible role of the Oriental hornet as a vector for honeybee viruses. Adult hornets were subjected to macroscopical examination to identify the presence of lesions, and to biomolecular investigation to detect the presence of six honeybee viruses: Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Deformed Wing Virus (DWV), Kashmir Bee Virus (KBV), Sac Brood Virus (SBV). No macroscopical alterations were found while biomolecular results showed that DWV was the most detected virus (25/30), followed by ABPV (19/30), BQCV (13/30), KBV (1/30) and SBV (1/30). No sample was found positive for CBPV. In 20/30 samples several co-infections were identified. The most frequent (17/30) was the association between DWV and ABPV, often associated to BQCV (9/17). One sample (1/30) showed the presence of four different viruses namely DWV, ABPV, BQCV and KBV. The detected viruses are the most widespread in apiaries across the Italian territory suggesting the possible passage from honeybees to V. orientalis, by predation of infected adult honeybees and larvae, and cannibalization of their carcasses. However, to date, it is still not clear if these viruses are replicative but we can suggest a role as mechanical vector of V. orientalis in spreading these viruses.

Published
2022
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43. Detection of <scp> Felis catus </scp> papillomavirus type‐2 DNA and viral gene expression suggest active infection in feline oral squamous cell carcinoma

Author

Leticia Elisa Bartolomé Del Pino, Antonella Cersini, Elisabetta Razzuoli, Claudia Eleni, Giuseppe Borzacchiello, Cristiano Cocumelli, Giusy Cardeti, Gennaro Altamura, Manuela Martano, Paola Maiolino, Altamura, Gennaro, Cardeti, Giusy, Cersini, Antonella, Eleni, Claudia, Cocumelli, Cristiano, Bartolomé Del Pino, Leticia Elisa, Razzuoli, Elisabetta, Martano, Manuela, Maiolino, Paola, and Borzacchiello, Giuseppe

Subjects
squamous cell carcinoma, 040301 veterinary sciences, mucosal, cat, Biology, Cat Diseases, Real-Time Polymerase Chain Reaction, 0403 veterinary science, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, oncogene, Gene expression, Animals, viral tropism, RNA, Messenger, Papillomaviridae, Gene, Messenger RNA, General Veterinary, Squamous Cell Carcinoma of Head and Neck, Papillomavirus Infections, 04 agricultural and veterinary sciences, Molecular biology, infection, Feline Oral Squamous Cell Carcinoma, chemistry, 030220 oncology & carcinogenesis, DNA, Viral, Cats, Tissue tropism, Etiology, Mouth Neoplasms, Viral load, DNA
Abstract

Papillomavirus (PV) infection is associated with development of epithelial cancer in different species, including domestic cat (Felis catus). Felis catus PV type-2 (FcaPV-2) is considered the causative agent of a proportion of feline cutaneous squamous cell carcinoma (SCC), through the transforming properties of its E6 and E7 oncogenes. However, the possible role of FcaPVs in the aetiology of feline oral SCC (FOSCC) is still unclear. The aim of this study was to assess the presence and gene expression of FcaPV-2 in FOSCC samples. We detected FcaPV-2 DNA in 10/32 (31%) of the analysed FOSCC by the use of PCR methods. Importantly, viral mRNA was detected by RT-PCR in 7/10 (70%) of DNA positive samples. In particular, FcaPV-2 L1, E2 and E6E7 genes were found to be expressed in 5/10 (50%), 3/10 (33%) and 5/10 (50%) samples, respectively. Viral DNA was also detected in non neoplastic oral ulcerative lesions (ULs) (4/11, 36%); qPCR suggested a difference in viral load between ULs and FOSCCs, particularly in those expressing E6E7, although it was not statistically significant. These data suggest, but do not definively prove, a possible role of FcaPV-2 in the development of a proportion of FOSCC. Moreover, L1 and E2 gene expression results indicate that FcaPV-2 infection associated with these tumours may possibly be productive.

Published
2020
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46. VESPA ORIENTALIS: A POSSIBLE VECTOR FOR HONEYBEE PATHOGENS

Author

KAREN POWER, TECLA TOSCANO, GENNARO ALTAMURA, PATRIZIO CATALANO, MANUELA MARTANO, PAOLA MAIOLINO, Parco Nazionale Pantelleria, Power, Karen, Toscano, Tecla, Altamura, Gennaro, Catalano, Patrizio, Martano, Manuela, and Maiolino, Paola

Published
2022

50. Why Shouldn't Veterinary Pathologists Be Interested in Honeybee Pathology?

Author

Paola Maiolino, Karen Power, Manuela Martano, Power, Karen, Martano, Manuela, and Maiolino, Paola

Subjects
Pathologists, medicine.medical_specialty, General Veterinary, Family medicine, medicine, MEDLINE, Animals, Humans, RNA Viruses, Biology, Bees, Infections, Veterinarians
Published
2020

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