62 results on '"Mabrouk Elsabagh"'
Search Results
2. Fruit processing by‐products in the aquafeed industry: A feasible strategy for aquaculture sustainability
- Author
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Mahmoud A. O. Dawood, Ola A. E. Habotta, Mabrouk Elsabagh, Mohamad Nor Azra, Hien Van Doan, Zulhisyam Abdul Kari, and Hani Sewilam
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Ecology ,Management, Monitoring, Policy and Law ,Aquatic Science - Published
- 2022
3. Seroepidemiological survey of the Crimean-Congo Hemorrhagic Fever Virus (CCHFV) infection amongst domestic ruminants in Adana province, East Mediterranean, Turkey
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Bilge Kaan TEKELİOĞLU, Emre OZAN, Armağan Erdem ÜTÜK, Ayça Hatice ATLI, Harun ALBAYRAK, Mabrouk ELSABAGH, Mahmut GÖKÇE, Nevin TURUT, Ömer Memduh ESENDAL, and Mehmet ÇELİK
- Subjects
General Medicine - Abstract
Crimean-Congo hemorrhagic fever virus (CCHFV) has been observing since 2002 and 2015 in Turkey and Adana, respectively. However, there were no previous seroepidemiological data on CCHFV in Adana. This study, thus, aimed to investigate the seroepidemiological status of Adana province for CCHFV in ruminants, according to species, breed, age, gender and geographical location. The study was carried out in 15 districts from sea level to plateaus (0-1472 m), within a 14,030 km² area. 485 samples were tested with modified anti-CCHFV IgG ELISA. A human-based IgG ELISA was adapted to recognize cattle, sheep and goats IgG antibodies. The conjugation (anti-human IgG peroxidase) was replaced with appropriate conjugate in each species. Statistical analyses indicated a significant relationship between antibody presence and animal age, species, breed and location but there was no relation with animal gender. Out of 485 samples, 154 were seropositive (31.8%). It was determined that the statistical cluster varied between 5.8% and 74.3% according to location and seropositivity. The most important variable associated with antibody presence was geographic location. While high seropositivity is found at altitudes above 555 meters, it is found to be 7% and 3% at sea level. The seropositivity reached 100% in the village where human deaths were seen in 2015, and 74.3% in the Tufanbeyli district where this village is located. The results indicate that Adana is an endemic province for CCHFV infection, especially in high altitude regions.
- Published
- 2021
4. Dietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction
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Hao Zhang, Ying Zhang, Yi Ma, Mengzhi Wang, Mabrouk Elsabagh, and Hongrong Wang
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medicine.medical_specialty ,medicine.medical_treatment ,L-arginine ,Intrauterine growth restriction ,SF1-1100 ,Liver inflammation ,chemistry.chemical_compound ,Food Animals ,Internal medicine ,N-carbamylglutamate ,medicine ,Original Research Article ,Fetus ,Triglyceride ,Cholesterol ,business.industry ,Interleukin ,medicine.disease ,Animal culture ,Endocrinology ,Cytokine ,chemistry ,Gestation ,Animal Science and Zoology ,Fetal sheep ,business ,Transforming growth factor - Abstract
This study aimed to explore whether dietary rumen-protected L-arginine (RP-Arg) or N-carbamylglutamate (NCG) supplementation to feed-restricted pregnant ewes counteracts fetal hepatic inflammation and innate immune dysfunction associated with intrauterine growth retardation (IUGR) in ovine fetuses. On d 35 of pregnancy, twin-bearing Hu ewes (n = 32) were randomly assigned to 4 treatment groups (8 ewes and 16 fetuses per group) and fed diets containing 100% of the NRC requirements (CON), 50% of the NRC requirements (RES), RES + RP-Arg (20 g/d) (RESA), or RES + NCG (5 g/d) (RESN). At 08:00 on d 110 of gestation, fetal blood and liver tissue samples were collected. The levels of triglyceride, free fatty acid, cholesterol and β-hydroxybutyrate in the fetal blood of RESA and RESN groups were lower (P
- Published
- 2021
5. Dietary L-Arginine or N-Carbamylglutamate Alleviates Colonic Barrier Injury, Oxidative Stress, and Inflammation by Modulation of Intestinal Microbiota in Intrauterine Growth-Retarded Suckling Lambs
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Hao Zhang, Yi Zheng, Xia Zha, Yi Ma, Xiaoyun Liu, Mabrouk Elsabagh, Hongrong Wang, and Mengzhi Wang
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intrauterine growth restriction ,Physiology ,inflammation ,Clinical Biochemistry ,N-carbamylglutamate ,L-arginine ,suckling lambs ,Cell Biology ,Molecular Biology ,Biochemistry ,colonic barrier function - Abstract
Our previous studies have revealed that dietary N-carbamylglutamate (NCG) and L-arginine (Arg) supplementation improves redox status and suppresses apoptosis in the colon of suckling Hu lambs with intrauterine growth retardation (IUGR). However, no studies have reported the function of Arg or NCG in the colonic microbial communities, barrier function, and inflammation in IUGR-suckling lambs. This work aimed to further investigate how dietary Arg or NCG influences the microbiota, barrier function, and inflammation in the colon of IUGR lambs. Forty-eight newborn Hu lambs of 7 d old were assigned to four treatment groups (n = 12 per group; six male, six female) as follows: CON (normal birth weight, 4.25 ± 0.14 kg), IUGR (3.01 ± 0.12 kg), IUGR + Arg (2.99 ± 0.13 kg), and IUGR + NCG (3.03 ± 0.11 kg). A total of 1% Arg or 0.1% NCG was supplemented in a basal diet of milk replacer, respectively. Lambs were fed the milk replacer for 21 d until 28 d after birth. Compared to the non-supplemented IUGR lambs, the transepithelial electrical resistance (TER) was higher, while fluorescein isothiocyanate dextran 4 kDa (FD4) was lower in the colon of the NCG- or Arg-supplemented IUGR lambs (p < 0.05). The IUGR lambs exhibited higher (p < 0.05) colonic interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, reactive oxygen species (ROS), and malondialdehyde (MDA) levels than the CON lambs; the detrimental effects of IUGR on colonic proinflammatory cytokine concentrations and redox status were counteracted by dietary Arg or NCG supplementation. Both IUGR + Arg and IUGR + NCG lambs exhibited an elevated protein and mRNA expression of Occludin, Claudin-1, and zonula occludens-1 (ZO-1) compared to the IUGR lambs (p < 0.05). Additionally, the lipopolysaccharide (LPS) concentration was decreased while the levels of acetate, butyrate, and propionate were increased in IUGR + Arg and IUGR + NCG lambs compared to the IUGR lambs (p < 0.05). The relative abundance of Clostridium, Lactobacillus, and Streptococcus was lower in the colonic mucosa of the IUGR lambs than in the CON lambs (p < 0.05) but was restored upon the dietary supplementation of Arg or NCG to the IUGR lambs (p < 0.05). Both Arg and NCG can alleviate colonic barrier injury, oxidative stress (OS), and inflammation by the modulation of colonic microbiota in IUGR-suckling lambs. This work contributes to improving knowledge about the crosstalk among gut microbiota, immunity, OS, and barrier function and emphasizes the potential of Arg or NCG in health enhancement as feed additives in the early life nutrition of ruminants.
- Published
- 2022
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6. Effect of phytol in forage on phytanic acid content in cow’s milk
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Yuzo Kurokawa, Mabrouk Elsabagh, Taketo Obitsu, Toshihisa Sugino, and Renlong Lv
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phytanic acid ,milk ,General Veterinary ,Phytanic acid ,Physiology ,Silage ,food and beverages ,Total mixed ration ,Article ,Ruminant Nutrition and Forage Utilization ,chemistry.chemical_compound ,Rumen ,Phytol ,phytol ,Animal science ,chemistry ,QL1-991 ,Genetics ,Animal Science and Zoology ,Dry matter ,Lactose ,silage ,Somatic cell count ,Zoology ,Food Science - Abstract
Objective: Bioactive compounds in ruminant products are related to functional compounds in their diets. Therefore, this study aimed to explore the effect of forage sources, Italian ryegrass (IR) silage vs corn silage (CS) in the total mixed ration (TMR), on milk production, milk composition, and phytanic acid content in milk, as well as on the extent of conversion of dietary phytol to milk phytanic acid.Methods: Phytanic acid content in milk was investigated for cows fed a TMR containing either IR silage or CS using 17 cows over three periods of 21 days each. In periods 1 and 3, cows were fed CS-based TMR (30% CS), while in period 2, cows were fed IR silage-based TMR (20% IR silage and10% CS).Results: The results showed that there were no differences in fat, protein, lactose, solidsnot-fat, somatic cell count, and fatty acid composition of milk among the three experimental periods. There were no differences in the plasma concentration of glucose, triglycerides, total cholesterol, and nonesterified fatty acids among the three experimental periods, while the blood urea nitrogen was higher (p
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- 2021
7. Dietary N-carbamylglutamate or l-arginine supplementation improves hepatic energy status and mitochondrial function and inhibits the AMP-activated protein kinase-peroxisome proliferator-activated receptor γ coactivator-1α-transcription factor A pathway in intrauterine-growth-retarded suckling lambs
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Shengnan Ren, Hao Zhang, Hongrong Wang, Xiaoyun Liu, Mabrouk Elsabagh, and Mengzhi Wang
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l-Arginine ,medicine.medical_specialty ,Arginine ,Peroxisome proliferator-activated receptor ,Intrauterine growth restriction ,SF1-1100 ,Food Animals ,AMP-activated protein kinase ,Internal medicine ,medicine ,N-carbamylglutamate ,Citrate synthase ,Original Research Article ,Receptor ,Energy status ,chemistry.chemical_classification ,biology ,TFAM ,Adenosine ,Animal culture ,Isocitrate dehydrogenase ,Endocrinology ,chemistry ,Liver ,biology.protein ,Animal Science and Zoology ,Mitochondrial function ,medicine.drug - Abstract
The objective of this study was to investigate the effects of dietary administration of l-arginine (Arg) or N-carbamylglutamate (NCG) on hepatic energy status and mitochondrial functions in suckling Hu lambs with intrauterine growth retardation (IUGR). Forty-eight newborn Hu lambs of 7 d old were allocated into 4 treatment groups of 12 lambs each, in triplicate with 4 lambs per replicate (2 males and 2 females) as follows: CON (lambs of normal birth weight, 4.25 ± 0.14 kg), IUGR (3.01 ± 0.12 kg), IUGR + 1% Arg (2.99 ± 0.13 kg), or IUGR + 0.1% NCG (3.03 ± 0.11 kg). The experiment lasted for 21 d, until d 28 after birth, and all lambs were fed milk replacer as a basal diet. Compared with IUGR lambs, NCG or Arg administration increased (P
- Published
- 2021
8. Effects of formic acid and corn flour supplementation of banana pseudostem silages on nutritional quality of silage, growth, digestion, rumen fermentation and cellulolytic bacterial community of Nubian black goats
- Author
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Bo Lin, Hongrong Wang, Xuan Cheng, Hao Zhang, and Mabrouk Elsabagh
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0106 biological sciences ,Silage ,Formic acid ,Agriculture (General) ,Plant Science ,01 natural sciences ,Biochemistry ,S1-972 ,nutrient digestion ,chemistry.chemical_compound ,Rumen ,Animal science ,Food Animals ,Dry matter ,banana pseudostem ,Completely randomized design ,Ecology ,Chemistry ,goat ,food and beverages ,04 agricultural and veterinary sciences ,ruminal cellulolytic bacteria ,Carbohydrate ,rumen fermentation ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Animal Science and Zoology ,Fermentation ,Digestion ,silage ,Agronomy and Crop Science ,010606 plant biology & botany ,Food Science - Abstract
The objective of this study was to evaluate the effects of adding formic acid and corn flour supplementation to banana pseudostem silages on the nutritional quality of these silages, growth, digestion, rumen fermentation and cellulolytic bacterial community of Nubian black goats fed these silages. Banana pseudostem silage was prepared either conventionally without any additives (CON) or mixed with 0.6% formic acid (F), 10% corn flour (C), or both (F+C). Four experimental diets containing 40% of the corresponding silages were designed with roughage to concentrate ratio of 50:50 (dry matter (DM) basis). A total of 48 Nubian black castrated goats (body weight (BW), (22.64±1.82) kg; 4-mon-old) were randomized into one of the four treatment groups with 12 replicates of one castrated goat per replicate for each treatment in a completely randomized design. Each group was fed on one of the four experimental diets for 40 days. A factorial arrangement of treatments of 2 (formic acid levels: 0 and 0.6%)×2 (corn flour: 0 and 10%) was adopted. Formic acid supplementation increased (P0.05) for any of the other indices except for the apparent digestibility of non-fibrous carbohydrate (NFC) (P
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- 2021
9. Effects of the Gut Microbiota and Barrier Function on Melatonin Efficacy in Alleviating Liver Injury
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Hao Zhang, Xiaoyun Liu, Mabrouk Elsabagh, Ying Zhang, Yi Ma, Yaqian Jin, Mengzhi Wang, Hongrong Wang, and Honghua Jiang
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Physiology ,Clinical Biochemistry ,cadmium ,melatonin ,intestinal microbiota ,liver injury ,gut microbiota transplantation ,Cell Biology ,Molecular Biology ,Biochemistry - Abstract
Environmental cadmium (Cd) exposure has been associated with severe liver injury. In contrast, melatonin (Mel) is a candidate drug therapy for Cd-induced liver injury due to its diverse hepatoprotective activities. However, the precise molecular mechanism by which Mel alleviates the Cd-induced liver injury, as well as the Mel–gut microbiota interaction in liver health, remains unknown. In this study, mice were given oral gavage CdCl2 and Mel for 10 weeks before the collection of liver tissues and colonic contents. The role of the gut microbiota in Mel’s efficacy in alleviating the Cd-induced liver injury was evaluated by the gut microbiota depletion technique in the presence of antibiotic treatment and gut microbiota transplantation (GMT). Our results revealed that the oral administration of Mel supplementation mitigated liver inflammation, endoplasmic reticulum (ER) stress and mitophagy, improved the oxidation of fatty acids, and counteracted intestinal microbial dysbiosis in mice suffering from liver injury. It was interesting to find that neither Mel nor Cd administration induced any changes in the liver of antibiotic-treated mice. By adopting the GMT approach where gut microbiota collected from mice in the control (CON), Cd, or Mel + Cd treatment groups was colonized in mice, it was found that gut microbiota was involved in Cd-induced liver injury. Therefore, the gut microbiota is involved in the Mel-mediated mitigation of ER stress, liver inflammation and mitophagy, and the improved oxidation of fatty acids in mice suffering from Cd-induced liver injury.
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- 2022
10. Changes of photosynthetic pigments and phytol content at different levels of nitrogen fertilizer in Italian ryegrass fresh herbage and hay
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Taketo Obitsu, Mabrouk Elsabagh, Renlong Lv, Yuzo Kurokawa, Toshihisa Sugino, and Kensuke Kawamura
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chemistry.chemical_element ,Plant Science ,Photosynthesis ,Nitrogen ,Pigment ,Phytol ,chemistry.chemical_compound ,Nitrogen fertilizer ,chemistry ,Agronomy ,visual_art ,Chlorophyll ,visual_art.visual_art_medium ,Hay ,Agronomy and Crop Science ,Ecology, Evolution, Behavior and Systematics - Published
- 2021
11. The protective effects of 18β-glycyrrhetinic acid against acrylamide-induced cellular damage in diabetic rats
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Mabrouk Elsabagh, Mohamed Emam, Mohamed M. Abdel-Daim, Ibtesam S. Alanazi, and Saad Alkahtani
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musculoskeletal diseases ,medicine.medical_specialty ,Antioxidant ,endocrine system diseases ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,chemistry.chemical_compound ,immune system diseases ,Internal medicine ,Diabetes mellitus ,medicine ,Environmental Chemistry ,skin and connective tissue diseases ,0105 earth and related environmental sciences ,Creatinine ,Cholesterol ,business.industry ,nutritional and metabolic diseases ,General Medicine ,Glutathione ,Streptozotocin ,medicine.disease ,Pollution ,Endocrinology ,chemistry ,Acrylamide ,business ,Oxidative stress ,medicine.drug - Abstract
This study was aimed at elucidating the protective effects of 18β-glycyrrhetinic acid (18βGA) against acrylamide (Acr)-induced cellular damage in diabetic rats. Rats were randomly assigned into eight groups (n = 8) following 12 h of fasting: control group, a single dose of 50 mg/kg streptozotocin (STZ) intraperitoneally (diabetic group), 50 mg/kg 18βGA orally after 2 weeks from STZ injection (18βGA group), 20 mg/kg Acr after 1month from STZ injection (Acr group), STZ plus Acr (STZ-Acr group), STZ plus 18βGA (STZ-18βGA group), Acr plus 18βGA (Acr-18βGA group), or STZ plus Acr plus 18βGA (STZ-Acr-18βGA group). Administration of 18βGA alone increased GSH, GSH-PX, SOD, and CAT in both liver and kidneys. While STZ injection was associated with diabetic and oxidative stress changes as indicated by the higher serum glucose, cholesterol, creatinine, IL-1β, IL-6, TNF-α, and antioxidant enzyme activities, together with increased lipid peroxides and decreased antioxidant biomarkers in the liver and kidneys. Similarly, the co-administration of STZ and Acr was associated with similar, more augmented effects, compared to STZ alone. The administration of 18βGA normalized STZ and Acr-induced elevations in oxidative defense variables in the liver and kidney tissues and blood biomarkers. Thus, our study demonstrated that the damaging effects of Acr were more exaggerated in diabetic rats. Furthermore, it showed the ability of 18βGA to inhibit reactive oxygen species generation and restore the antioxidant defenses in diabetic rats with Acr-induced liver and kidney cytotoxicity.
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- 2021
12. The Incidence of Heat Stress on the Quality of Food Crops in the Era of the Changing Climate
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Ayman EL Sabagh, Celaleddin Barutçular, Akbar Hossain, Fatih Çiğ, Murat Erman, Emine Karademir, Mohammad Sohidul Islam, Swapan Kumar Paul, Allah Wasaya, Muhammad Aamir Iqbal, Sytar Oksana, Ntsomboh-Ntsefong Godswill, Shah Fahad, Muhammad Mubeen, Hamid Majeed, Wajid Nasim, and Mabrouk Elsabagh
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- 2022
13. Current Anatolian Water Buffalo (Bubalus bubalis) Husbandry Practices at Igdir Province, Turkey
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Onur Şahin, Mabrouk Elsabagh, and Isa Yilmaz
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Veterinary medicine ,Geography ,biology ,Milk intake ,Water buffalo ,Herd ,Weaning ,Colostrum ,Bubalus ,Animal husbandry ,biology.organism_classification ,Insemination - Abstract
This study was conducted as a survey study in the 2017 (from September to December) to analyze the current status and husbandry practices of Anatolian water buffalo enterprises in Igdir, Turkey. Using the Simple Random Sampling procedure, a sample of 98 enterprises was selected among 319 Anatolian water buffalo producers to apply a questionnaire as a primary source of data collection. The data revealed that the average age of the owners was 56.87±1.27 years and they are literate and have mixed enterprises. The first age of the breeding of buffalo, postpartum service internal, insemination after estrus detection, lactation length and the number of inseminations per gestation were 28.20±0.67 months, 72.47±2.82 days, 2.91±0.23 hours, 236.74 days and 1.29±0.06 times, respectively. The average intake time of first colostrum, the amount of milk intake within the first two hours after birth and the weaning age in the Anatolian water buffalo calves were 1.59±0.72 hours, 2.71±0.97 kg and 208.42±48.25, respectively. Implementation of effective policies to develop the breeding of Anatolian water buffalo in Igdir is mandatory. Such policies include extension services (on nutrition, veterinary care, product processing…etc.), increasing the herd numbers, and establishing a union for Anatolian Water Buffalo Breeders in Igdir province.
- Published
- 2021
14. Autophagy attenuates placental apoptosis, oxidative stress and fetal growth restriction in pregnant ewes
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Hao Zhang, Yi Zheng, Xiaoyun Liu, Xia Zha, Mabrouk Elsabagh, Yi Ma, Honghua Jiang, Hongrong Wang, and Mengzhi Wang
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General Environmental Science - Published
- 2023
15. Efficacy of Bacillus probiotic mixture on the immunological responses and histopathological changes of Nile tilapia ( Oreochromis niloticus , L) challenged with Streptococcus iniae
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Mahmoud Eltholth, Eman M. Moustafa, Olivier Decamp, Ahmad Hamza, Khalid Shahin, Mahmoud A.O. Dawood, Amira A. Omar, Foad Farrag, Mabrouk Elsabagh, and Radi A. Mohamed
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Bacillus (shape) ,biology ,business.industry ,Aquatic Science ,biology.organism_classification ,Microbiology ,law.invention ,Oreochromis ,Nile tilapia ,Probiotic ,Aquaculture ,law ,Streptococcus iniae ,business - Abstract
Isolates of Streptococcus iniae were recovered from the diseased fish during summer season, identified and characterized using different laboratory techniques. Three hundred and sixty Nile tilapia (100 ± 5.0 g average weight) were divided into two subgroups in triplicates with 20 fish per replicate and received either sterile PBS (B0, B1 and B2, respectively) or S. iniae (B0+ S. iniae, B1+ S. iniae and B2 S. iniae, respectively). At 15 days post‐challenge, results showed low mortality rates in probiotic‐fed groups. The mortalities ranged from 6.67% in B2 to 81.67% in B0 following S. iniae challenge. The haematological parameters showed a significant increase (p < 0.05) in haemoglobin, red blood cells and white blood cells in B1, B1+S. iniae, B2 and B2+ S. iniae groups compared to B0+ S. iniae group. The alanine aminotransferase (ALT) level was elevated (p < 0.05) in tilapia challenged with S. iniae compared to the control groups. Glucose and creatinine levels were elevated (p < 0.05) in all groups compared with B0. Both albumin and globulin levels were significantly low (p < 0.05) in B0+ S. iniae compared to other groups. The total protein level, phagocytosis and phagocytic index were significantly lower (p < 0.05) in B0+ S. iniae than other groups. Histological analysis showed that the hepatopancreas of B0, B1 and B2 groups represents normal hepatocyte architecture, while the infected tilapia showed severe diffused necrosis, mononuclear cell infiltration and loss of the normal architecture. Spleen of PBS control groups did not show any pathological changes, while that of infected tilapia revealed several necrotic areas in addition to marked depletion of lymphoid cells. A loss of skin layers was observed in infected tilapia with marked interstitial oedema and leucocytic cell infiltration which was not observed in the probiotic‐fed fish. The current study highlights the immunomodulatory effect of Bacillus probiotic mixture against S. iniae infection in tilapia that can be further applied to control the disease in farms.
- Published
- 2020
16. Use of Poultry By-product and Plant Protein Sources in Diets of Redclaw Crayfish (Cherax quadricarinatus)
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Orhan Tufan Eroldogan, Mabrouk Elsabagh, Hüseyin Sevgili, Brett Glencross, Marina Paolucci, Metin Kumlu, Enes Kınay, Ece Evliyaoğlu, Hatice Asuman Yılmaz, and Merve Sarıipek
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food and beverages ,Animal Science and Zoology ,Aquatic Science - Abstract
A total of 300 juvenile crayfish (13.0±0.03 g) were randomly distributed among 5 dietary groups (n=60, 3 replicates) held within 15×500 L-1 fiberglass tanks connected to a recirculation system (RAS), at 20 crayfish per tank. Each group was fed for 12 weeks one of five experimental diets where the main protein sources were: 1) control, fish-meal-based diet (FM, 48% of the diet); 2) 10% FM + 52.5% poultry by-product meal (PoM); 3) 34.5% soybean meal + 34.5% corn gluten meal (Pmix); 4) 34.5% PoM + 32.soybean/corn gluten meal mix (PoM/Pmix); and 5) 10% FM + 27.5% soybean + 27.5% corn gluten meal (FM/Pmix). The results demonstrated that there were no significant differences among diets in terms of growth and feed utilization efficiency. Muscle amino acid profile of redclaw crayfish fed the FM diet had the highest level of total essential amino acids, followed by FM/PMix, Pmix, PoM/Pmix, and PoM diets. Particularly, in all experimental groups, the highest essential amino acids (EAA) were lysine, arginine, and leucine. Based on these findings, we conclude that redclaw can perform well with FM-free vegetable diets and PoM-based diets although more research is needed into the total composition of EAA and FA in muscle.
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- 2022
17. Determination of the trace minerals requirements for maintenance and growth of 35–50 kg Dorper × Hu crossbred ram lambs
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Feng Wang, Mabrouk Elsabagh, Along Peng, Nthanda C. Amini, Juan J. Loor, Ying Zhang, Haitao Nie, and Hao Zhang
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040301 veterinary sciences ,Trace mineral ,growth ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Biology ,Body weight ,SF1-1100 ,040201 dairy & animal science ,Crossbreed ,maintenance ,Animal culture ,0403 veterinary science ,Animal science ,Trace Minerals ,trace mineral ,Animal Science and Zoology ,comparative slaughter ,ram lambs - Abstract
This study aimed at estimating the trace minerals net requirements for maintenance and growth of Dorper × Hu ram lambs using the comparative slaughter techniques in 35 lambs of 35–50 kg body weight (BW). Seven lambs were slaughtered at the initial BW (34.93 ± 0.37 kg) to determine the basal whole-body composition. Another seven lambs were fed ad libitum (AL) and then slaughtered when it reached 41.73 ± 0.53 kg BW. The remained 21 lambs were assigned into three treatment groups, seven animals each, and fed 100, 70 or 40% of AL intake, respectively, and were slaughtered when they reached a BW of 49.93 ± 1.03 kg. The net requirements of maintenance were 5.1, 429.3, 94.0 and 48.8 µg/kg empty BW (EBW) for Mn, Fe, Zn and Cu, respectively. The net requirements of growth at 35 kg BW were 0.86, 70.41, 33.46 and 4.31 mg/kg EBW for Mn, Fe, Zn and Cu, respectively. At a BW of 50 kg, the net growth requirements were 0.93, 68.40, 35.20 and 4.15 mg/kg EBW for Mn, Fe, Zn and Cu, respectively. Our data indicated that the Mn and Zn requirements increase, whereas those of Fe and Cu decrease when BW increases. In addition, the net Cu, Mn and Fe requirements for maintenance and Zn, Cu, Mn and Fe requirements for growth of Dorper × Hu hybrid ram lambs were higher than those reported in the NRC but Zn requirements for maintenance matched that of the NRC.Highlights Trace minerals requirements for maintenance and growth vary with sheep genotype or breed. The comparative slaughter method could be a reliable tool for estimating the net requirements of trace minerals in Dorper × Hu ram lambs. Our estimated values for maintenance (Cu, Mn and Fe) and growth (Zn, Cu, Mn and Fe) requirements were higher than those reported in the NRC ().
- Published
- 2020
18. Dietary
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Hao, Zhang, Yi, Zheng, Xia, Zha, Xiaoyun, Liu, Yi, Ma, Juan J, Loor, Mabrouk, Elsabagh, Mengzhi, Wang, Hongrong, Wang, and Honghua, Jiang
- Abstract
Previous studies have revealed that dietary
- Published
- 2021
19. Effect of unconventional by-product on growth performance, digestibility, carcass characteristics, blood profile and cecal microbial activity in New Zealand white rabbits
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Yasmin M.M. Mahmoud, Mabrouk Elsabagh, and Abd El-Kader Mahmoud Kholif
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Animal science ,By-product ,New zealand white ,Biology - Abstract
This study addressed the effect of unconventional by-products on growth performance, carcass characteristics, some blood parameters, and caecal activities in New Zealand White (NZW) rabbits. A total of 48 weaned NZW rabbits (748 ± 9.50 g BW, 6 weeks old), randomly assigned into three treatment groups (16 each, individually housed), were fed a basal diet containing BBP at 0.00 (Control), 1.00 (Broc1) or 3.00 (Broc2) % on a dry matter (DM) basis as feed additives, for 8 weeks. The Broc2 rabbits showed the highest (P < 0.05) body weight gain and feed intake followed by the Broc1 group compared to the control one. Feed conversion ratio and performance index did not differ (P > 0.05) among treatments. Nutrient digestibility was higher (P < 0.05) for Broc1 and Broc2 rabbits compared to the control. Both Broc1 and Broc2 rabbits showed higher (P < 0.05) empty edible carcass, giblets, dressing, head, liver, heart and kidney weights, but showed a lower non-edible carcass weight compared to the control. Blood proteins and liver function enzymes were increased (P < 0.05) whereas the glucose, total cholesterol, uric acid and creatinine were decreased (P < 0.05) in Broc1 and Broc2 rabbits compared to the control. The caecal concentration of total volatile fatty acids was increased (P < 0.05) but that of ammonia was decreased (P < 0.05) with Broc1 and Broc2 groups of rabbits compared to those of control. Thus, dietary supplementation of BBP at 3% of DM in rabbits’ diets could improve their growth performance, carcass traits, liver function and blood profile as well as the caecal fermentation parameters.
- Published
- 2021
20. Recent advances in the utilization of insects as an ingredient in aquafeeds: A review
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Sahya Maulu, Sandra Langi, Oliver J. Hasimuna, Dagoudo Missinhoun, Brian P. Munganga, Buumba M. Hampuwo, Ndakalimwe Naftal Gabriel, Mabrouk Elsabagh, Hien Van Doan, Zulhisyam Abdul Kari, and Mahmoud A.O. Dawood
- Subjects
Food Animals ,Animal Science and Zoology - Abstract
The aquafeed industry continues to expand in response to the rapidly growing aquaculture sector. However, the identification of alternative protein sources in aquatic animal diets to replace conventional sources due to cost and sustainability issues remains a major challenge. Recently, insects have shown tremendous results as potential replacers of fishmeal in aquafeed. The present study aimed to review the utilization of insects in aquafeeds and their effects on aquatic animals' growth and feed utilization, immune response and disease resistance, and fish flesh quality and safety. While many insect species have been investigated in aquaculture, the black soldier fly (
- Published
- 2021
21. World Nations Priorities on Climate Change and Food Security
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Hafiz Muhammad Rashad Javeed, Wajid Nasim, Muhammad Anjum Ali, Muhammad Tahir, Nazim Hussain, Ghulam Mustafa Shah, Sajjad Hussain, M. Ali, Mabrouk Elsabagh, Saeed Ahmad Qaisrani, Ashfaq Ahmad, Muhammad Sami Ul Din, Muhammad Mubeen, Muhammad Anwar-ul-Haq, and Ayman El Sabagh
- Subjects
Food chain ,Geography ,Food security ,Natural resource economics ,Food systems ,Climate change ,World population ,Dependant ,Livelihood ,Water scarcity - Abstract
The present food system (including production, transportation, processing, packaging, storing, retail, and consumption) is a source of nutrition for the great majority of the world population in addition to supporting the livelihoods of about 200 million people. Food supply per capita has increased by more than 30% since 1961, this is accompanied by more use of nitrogen fertilizers (showing an increase of about 800%) and water resources for irrigation (with an increase of more than 100%). Global food security will continue to be an international concern for the coming 50 years and even beyond. Crop yield has fallen in many areas recently due to decreasing investments in infrastructure and research, as well as due to growing water scarcity. Climate change is a global concern irrespective of borders. The poor nations are highly vulnerable to climate change and are at high risk. Food security is directly dependant on the food chain and the associated food system process. All dimensions of food security could be affected by climate change in complex ways. Approximately, 15 countries are highly vulnerable to food insecurity due to climate change, from Asia and Africa. Most of these nations are not able to cope with or counter the impact of climate change on an urgent basis. However, some countries have developed their national strategies and adaptation plans to alleviate the negative impacts of climate change.
- Published
- 2021
22. Evaluating the rumen‐protected lysine stability in forage‐based total mixed rations in vitro and determining the lysine Brix value
- Author
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Taketo Obitsu, Atsushi Haruno, Maki Nakamura, Mabrouk Elsabagh, Seiichi Saiki, Izuru Shinzato, Hidetada Funo, Toshihisa Sugino, and Saki Ishimaru
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0303 health sciences ,Brix ,Rumen ,Time Factors ,Chemistry ,Lysine ,Temperature ,0402 animal and dairy science ,Water ,04 agricultural and veterinary sciences ,General Medicine ,Total mixed ration ,In Vitro Techniques ,Animal Feed ,040201 dairy & animal science ,In vitro ,Diet ,03 medical and health sciences ,Animal science ,Animals ,Dry matter ,General Agricultural and Biological Sciences ,030304 developmental biology - Abstract
Three rumen-protected lysine (RPL) products (AjiPro® -L, LysiPEARLTM , and Feedtech Bypass LysineTM : A, B, and C, respectively) were tested for stability in two forage-based total mixed rations (TMR1, 41.3% dry matter (DM), and TMR2, 49.5% DM) (experiment 1) and for Brix value (experiment 2). In experiment 1, each RPL product (2 g each) and TMR diet (200 g) were mixed and stored in plastic bags at 20°C for 0, 1, 3, 6, 12, 24, and 48 hr. In experiment 2, each RPL product (2 g) was dispensed into ion-exchanged water (20 ml) and kept at 20°C for 0, 0.5, 1, 3, 6, 12, 24, and 48 hr. At each time point, free lysine (Lys) content and Brix values of extracts were measured, and Lys release (LR, %) was calculated. All RPL products LR% varied with varying diets DM and increased with increasing of time exposed to diets; it was highest in C, followed by B, and then A. Water LR% positively correlated with that from diets and with Brix values of Lys dissociated in water. Our results indicated that Lys dissociation from RPL products is affected by diet DM content. Brix value may be used as a potential marker for RPL protection efficacy.
- Published
- 2019
23. Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens
- Author
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Azza Hafez, Elsayed Hegazi, Abdelnasser Bakr, Mabrouk Elsabagh, Mohamed Fahmy, and Eldsokey Nassef
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Cellular immunity ,Antioxidant ,Health, Toxicology and Mutagenesis ,Lymphocyte ,medicine.medical_treatment ,010501 environmental sciences ,01 natural sciences ,Antioxidants ,Superoxide dismutase ,chemistry.chemical_compound ,Immune system ,Animal science ,medicine ,Animals ,Environmental Chemistry ,0105 earth and related environmental sciences ,biology ,Broiler ,General Medicine ,Malondialdehyde ,Animal Feed ,Pollution ,Diet ,medicine.anatomical_structure ,chemistry ,Dietary Supplements ,biology.protein ,Immunoglobulin Y ,Zinc Oxide ,Chickens - Abstract
This study aimed to elucidate the response of broiler chickens to the dietary nano-zinc supplementation in terms of immune response and antioxidant activity. Ninety-one-day-old chicks (Ross 308) were randomly assigned to one of three dietary treatments in three replicates, in a feeding trial that lasted for 5 weeks. Birds were fed a basal diet supplemented with inorganic zinc oxide at 40 mg/kg diet (control), zinc oxide nanoparticles (ZnONPs) at 40 mg/kg diet (ZN1), or ZnONPs at 80 mg/kg diet (ZN2). Birds were injected with DNP-KLH at the 7th and 21st days from the beginning of the experiment, and blood samples were collected on days 7, 14, 21, 28, and 35 to determine the levels of immunoglobulin Y (IgY) and malondialdehyde as well as the antioxidant enzyme activities. Cellular immunity was assayed by estimation of phagocytic percentage and index of peripheral monocytes of blood and estimation of the T lymphocyte activity using a lymphocyte transformation test. The results showed that feeding broiler chickens a diet supplemented with ZnONPs increased (p
- Published
- 2019
24. A comparative study of four rumen buffering agents on productive performance, rumen fermentation and meat quality in growing lambs fed a total mixed ration
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Ibrahim A. Alhidary, Mabrouk Elsabagh, and Mutassim M. Abdelrahman
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Male ,sheep ,Rumen ,040301 veterinary sciences ,Total mixed ration ,Buffers ,ruminal health ,SF1-1100 ,Feed conversion ratio ,0403 veterinary science ,Butyric acid ,Awassi ,Eating ,chemistry.chemical_compound ,diet buffering capacity ,Animal science ,carcass traits ,Ruminant ,feed efficiency ,Animals ,Sodium bicarbonate ,biology ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Hydrogen-Ion Concentration ,Fatty Acids, Volatile ,biology.organism_classification ,Animal Feed ,040201 dairy & animal science ,Diet ,Animal culture ,Red Meat ,Sodium Bicarbonate ,chemistry ,Buffering agent ,Dietary Supplements ,Fermentation ,Animal Science and Zoology - Abstract
Controlling rumen fermentation using buffering agents could contribute to enhancing ruminant productivity and performance. This study was realized to investigate the effect of dietary supplementation of AcidBuf, sodium bicarbonate, calseapowder and WMC seaweed (Utva Lactuca extra) on the animal performance, volatile fatty acids, rumen pH, rumen histology and carcass characteristics of growing male Awassi lambs. A total of 60 lambs was divided into five groups. One group served as a control and fed only on a concentrate diet without any buffering, whereas the other four groups were fed the concentrate diet supplemented with 0.4% AcidBuf (Buf1), 0.4% AcidBuf plus sodium bicarbonate, 50 : 50 (Buf2), 0.4% calseapowder (Buf3) or 0.4% WMC Seaweed (Buf4) for 98 days. The feed conversion ratio was (P
- Published
- 2019
25. Effects of the maternal gut microbiome and gut-placental axis on melatonin efficacy in alleviating cadmium-induced fetal growth restriction
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Hao Zhang, Xiaoyun Liu, Yi Zheng, Xia Zha, Mabrouk Elsabagh, Ying Zhang, Yi Ma, Juan J. Loor, Mengzhi Wang, and Hongrong Wang
- Subjects
Fetal Growth Retardation ,Placenta ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,Pollution ,Gastrointestinal Microbiome ,Mice ,Pregnancy ,Animals ,Humans ,Female ,Cadmium ,Melatonin - Abstract
Cadmium (Cd) is a major environmental stressor that induces fetal growth restriction (FGR). Also, changes in gut microbiome diversity-which can be modulated positively by melatonin (Mel) have implications on fetal development and placental functions. Therefore, this study aimed to explore whether the role of Mel in counteracting the Cd-induced FGR by regulating placental barrier injury, endoplasmic reticulum stress (ERS) and mitophagy in pregnant mice is mediated-in part- via the gut microbiota modulations. Pregnant mice were intraperitoneally injected with CdCl
- Published
- 2022
26. Maize Adaptability to Heat Stress under Changing Climate
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Muhammad Ikram, Mohammad Sohidul Islam, Marian Brestic, Oksana Sytar, Allah Wasaya, Shah Fahad, Fatih Cig, Sobhy Sorour, Ram Swaroop Meena, Ömer Konuşkan, Mabrouk Elsabagh, Hirofumi Saneoka, Hakki Akdeniz, Wajid Nasim, Ayman El Sabagh, Muhammad Mubeen, Liyun Liu, Murat Erman, Akbar Hossain, Muhammad Rizwan, Muhammad Aamir Iqbal, Faraz Azeem, Ferhat Kizilgeçi, Habib-ur-Rehman Athar, Celaleddin Barutçular, Tasmiya Jabeen, Akihiro Ueda, and Maham Asif Bukhari
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0106 biological sciences ,media_common.quotation_subject ,InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL ,food and beverages ,04 agricultural and veterinary sciences ,Agricultural engineering ,Biology ,01 natural sciences ,Adaptability ,Heat stress ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,010606 plant biology & botany ,media_common - Abstract
The rapidly increasing human population is an alarming issue and would need more food production under changing climate. Abiotic stresses like heat stress and temperature fluctuation are becoming key issues to be addressed for boosting crop production. Maize growth and productivity are sensitive to temperature fluctuations. Grain yield losses in maize from heat stress are expected to increase owing to higher temperatures during the growing season. This situation demands the development of maize hybrids tolerant to heat and drought stresses without compromising grain yield under stress conditions. The chapter aimed to assess the updates on the influence of high-temperature stress (HTS) on the physio-biochemical processes in plants and to draw an association between yield components and heat stress on maize. Moreover, exogenous applications of protectants, antioxidants, and signaling molecules induce HTS tolerance in maize plants and could help the plants cope with HTS by scavenging reactive oxygen species, upregulation of antioxidant enzymes, and protection of cellular membranes by the accrual of compatible osmolytes. It is expected that a better thought of the physiological basis of HTS tolerance in maize plants will help to develop HTS maize cultivars. Developing HTS-tolerant maize varieties may ensure crops production sustainability along with promoting food and feed security under changing climate.
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- 2021
27. The protective effects of 18β-glycyrrhetinic acid against acrylamide-induced cellular damage in diabetic rats
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Ibtesam S, Alanazi, Mohamed, Emam, Mabrouk, Elsabagh, Saad, Alkahtani, and Mohamed M, Abdel-Daim
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Acrylamide ,Oxidative Stress ,Animals ,Glycyrrhetinic Acid ,Antioxidants ,Diabetes Mellitus, Experimental ,Rats - Abstract
This study was aimed at elucidating the protective effects of 18β-glycyrrhetinic acid (18βGA) against acrylamide (Acr)-induced cellular damage in diabetic rats. Rats were randomly assigned into eight groups (n = 8) following 12 h of fasting: control group, a single dose of 50 mg/kg streptozotocin (STZ) intraperitoneally (diabetic group), 50 mg/kg 18βGA orally after 2 weeks from STZ injection (18βGA group), 20 mg/kg Acr after 1month from STZ injection (Acr group), STZ plus Acr (STZ-Acr group), STZ plus 18βGA (STZ-18βGA group), Acr plus 18βGA (Acr-18βGA group), or STZ plus Acr plus 18βGA (STZ-Acr-18βGA group). Administration of 18βGA alone increased GSH, GSH-PX, SOD, and CAT in both liver and kidneys. While STZ injection was associated with diabetic and oxidative stress changes as indicated by the higher serum glucose, cholesterol, creatinine, IL-1β, IL-6, TNF-α, and antioxidant enzyme activities, together with increased lipid peroxides and decreased antioxidant biomarkers in the liver and kidneys. Similarly, the co-administration of STZ and Acr was associated with similar, more augmented effects, compared to STZ alone. The administration of 18βGA normalized STZ and Acr-induced elevations in oxidative defense variables in the liver and kidney tissues and blood biomarkers. Thus, our study demonstrated that the damaging effects of Acr were more exaggerated in diabetic rats. Furthermore, it showed the ability of 18βGA to inhibit reactive oxygen species generation and restore the antioxidant defenses in diabetic rats with Acr-induced liver and kidney cytotoxicity.
- Published
- 2020
28. β-Glucan improved the immunity, hepato-renal, and histopathology disorders induced by chlorpyrifos in Nile tilapia
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Mohamed Aboubakr, Ehab Yahya Abdelhiee, Mabrouk Elsabagh, Ali A. Soliman, Mahmoud A.O. Dawood, Mahmoud S. Gewaily, Bilal Ahamad Paray, Hani Sewilam, Hien Van Doan, Mohammed F. El Basuini, and Nagwa I. Abdel-Razik
- Subjects
medicine.medical_specialty ,Sustainable aquaculture ,Aquatic toxicology ,Aquatic Science ,Feed conversion ratio ,lcsh:Aquaculture. Fisheries. Angling ,03 medical and health sciences ,Nile tilapia ,chemistry.chemical_compound ,Internal medicine ,medicine ,ddc:630 ,030304 developmental biology ,Inflammation ,lcsh:SH1-691 ,0303 health sciences ,biology ,Immuno-toxicity ,04 agricultural and veterinary sciences ,biology.organism_classification ,Endocrinology ,chemistry ,Chlorpyrifos ,Toxicity ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Alkaline phosphatase ,Animal Science and Zoology ,Histopathology ,medicine.symptom ,Lysozyme ,Weight gain ,Functional feeds - Abstract
Aquaculture Reports 18, 100549 (2020). doi:10.1016/j.aqrep.2020.100549, Published by Elsevier, Amsterdam [u.a.]
- Published
- 2020
29. l-Arginine Alleviates Hydrogen Peroxide-Induced Oxidative Damage in Ovine Intestinal Epithelial Cells by Regulating Apoptosis, Mitochondrial Function, and Autophagy
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Hao Zhang, Mabrouk Elsabagh, Juan J. Loor, Yaotian Fan, Xiaoyun Liu, Along Peng, Hongrong Wang, and Yin Yu
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0301 basic medicine ,Arginine ,Medicine (miscellaneous) ,Apoptosis ,Mitochondrion ,medicine.disease_cause ,Protective Agents ,DNA, Mitochondrial ,03 medical and health sciences ,0302 clinical medicine ,Adenosine Triphosphate ,Mitophagy ,medicine ,Autophagy ,Animals ,Viability assay ,RNA, Messenger ,Intestinal Mucosa ,Cells, Cultured ,chemistry.chemical_classification ,Membrane Potential, Mitochondrial ,Reactive oxygen species ,Nutrition and Dietetics ,Sheep ,biology ,Cytochrome c ,Proteins ,Hydrogen Peroxide ,Molecular biology ,Mitochondria ,Oxidative Stress ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Dietary Supplements ,biology.protein ,Reactive Oxygen Species ,Oxidative stress - Abstract
BACKGROUND Previous studies demonstrated that dietary l-arginine (Arg) alters the equilibrium between reactive oxygen species (ROS) generation and biological defenses to resist oxidant-induced toxicity. Whether supplying Arg can protect ovine intestinal epithelial cells (OIECs) from hydrogen peroxide (H2O2)-induced oxidative damage is unclear. OBJECTIVES The current study aimed to examine the effect of Arg on mitophagy, mitochondrial dysfunction, and apoptosis induced by H2O2 in OIECs. METHODS The OIECs were incubated in Arg-free DMEM supplemented with 100 μM Arg (CON) or 350 μM Arg (ARG) alone or with 150 μM H2O2 (CON + H2O2, ARG + H2O2) for 24 h. Cellular apoptosis, mitochondrial function, autophagy, and the related categories of genes and proteins were determined. All data were analyzed by ANOVA using the general linear model procedures of SAS (SAS Institute) for a 2 × 2 factorial design. RESULTS Relative to the CON and ARG groups, H2O2 administration resulted in 44.9% and 26.5% lower (P
- Published
- 2020
30. Dietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction
- Author
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Hao Zhang, Mengzhi Wang, Yi Ma, Mabrouk Elsabagh, Hongrong Wang, and Juan J. Loor
- Subjects
0301 basic medicine ,medicine.medical_specialty ,GPX1 ,Arginine ,SOD2 ,Inflammation ,Superoxide dismutase ,03 medical and health sciences ,Random Allocation ,0302 clinical medicine ,Glutamates ,Enos ,Internal medicine ,medicine ,Animals ,Receptor ,Barrier function ,Fetal Growth Retardation ,Sheep ,biology ,Chemistry ,General Medicine ,biology.organism_classification ,Animal Feed ,Animals, Suckling ,030104 developmental biology ,Endocrinology ,030220 oncology & carcinogenesis ,embryonic structures ,Dietary Supplements ,biology.protein ,medicine.symptom ,Food Science - Abstract
The current work aimed at investigating the effects of the dietary supplementation of N-carbamylglutamate (NCG) or l-arginine (Arg) on the duodenal mitophagy, mitochondrial function, inflammation, and barrier function in suckling lambs suffering from intrauterine-growth-retardation (IUGR). Forty-eight neonate Hu lambs were used in this study: 12 lambs with normal birth weight (NBW: 4.25 ± 0.14 kg) and 36 lambs with IUGR (3.01 ± 0.13 kg). Seven day old lambs were assigned to 4 treatment groups (12 lambs in each group) as follows: control group (CON), IUGR group, IUGR + Arg, and IUGR + NCG. Lambs were fed the experimental diets for 21 days from 7 days to 28 days of age. Compared with IUGR lambs, the Arg or NCG-treated IUGR lambs had a markedly higher duodenal transepithelial electrical resistance (TER) and lower fluorescein isothiocyanate dextran (FD4) (P < 0.05), respectively. The duodenal mitochondrial membrane potential change (ΔΨm), relative mitochondrial DNA (mtDNA) content, adenosine triphosphate (ATP) level, together with the activities of the respiratory complexes I, III, and IV were markedly higher in Arg or NCG-treated IUGR lambs than those in non-supplemented IUGR lambs (P < 0.05). The expressions of the integrity-related proteins (occludin and zonula occludens-1 (ZO-1)), antioxidant- and apoptosis-related proteins (B-cell lymphoma/leukaemia 2 (Bcl2), superoxide dismutase 2 (SOD2), catalase (CAT), and glutathione peroxidase 1 (GPx1)), and the nitric oxide-dependent pathway-related proteins (epithelial NO synthase (eNOS) and inducible NO synthase (iNOS)) were higher in NCG or Arg-supplemented IUGR lambs than those in nontreated IUGR lambs (P < 0.05). The duodenal expressions of the mitophagy-related proteins (microtubule-associated protein light chain 3 (LC3) I, LC3 II, Belin1, PTEN induced putative kinase 1 (PINK1), and Parkin) and the immune function-related proteins (myeloid differentiation factor 88 (MyD88), IL-6, nuclear factor kappa B (p65), toll-like receptor (TLR4) and TNF-α) were reduced (P < 0.05) in NCG or Arg-supplemented IUGR lambs compared with non-supplemented IUGR lambs. These results demonstrated that the dietary supplementation of Arg or NCG enhanced the duodenal barrier function and mitochondrial function, mitigated duodenal inflammation, and suppressed mitophagy in suckling lambs suffering from IUGR.
- Published
- 2020
31. l-Arginine Inhibits Apoptosis of Ovine Intestinal Epithelial Cells through the l-Arginine-Nitric Oxide Pathway
- Author
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Along Peng, Hongrong Wang, Fangfang Zhao, Mabrouk Elsabagh, Mengzhi Wang, Hao Zhang, Shuang Guo, and Juan J. Loor
- Subjects
0301 basic medicine ,Arginine ,Medicine (miscellaneous) ,Apoptosis ,Mitochondrion ,Nitric Oxide ,Nitric oxide ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Animals ,Intestinal Mucosa ,Cells, Cultured ,chemistry.chemical_classification ,Reactive oxygen species ,Nutrition and Dietetics ,Sheep ,biology ,Chemistry ,Epithelial Cells ,Molecular biology ,Nitric oxide synthase ,030104 developmental biology ,NG-Nitroarginine Methyl Ester ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Nitric Oxide Pathway ,biology.protein - Abstract
BACKGROUND In nonruminants, many of the biological roles of l-arginine (Arg) at the intestinal level are mediated through the Arg-nitric oxide (Arg-NO) pathway. Whether the Arg-NO pathway is involved in controlling the immune response and viability in ovine intestinal epithelial cells (IOECs) is unclear. OBJECTIVES The current study aimed to examine the role of the Arg-NO pathway in apoptosis, antioxidant capacity, and mitochondrial function of IOECs. METHODS The IOECs were incubated in Arg-free DMEM supplemented with 150 μM Arg (CON) or 300 μM Arg (ARG) alone or with 350 μM Nw-nitro-l-arginine methyl ester hydrochloride (l-NAME) (CON + NAME, ARG + NAME) for 24 h. The reactive oxygen species (ROS) concentration, antioxidant capacity, and cell apoptotic percentage were determined. RESULTS Arg supplementation decreased (P
- Published
- 2020
32. Corrigendum
- Author
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Mabrouk Elsabagh
- Subjects
General Medicine ,General Agricultural and Biological Sciences - Published
- 2020
33. Dietary Supplementation of L-Arginine and N-Carbamylglutamate Attenuated the Hepatic Inflammatory Response and Apoptosis in Suckling Lambs with Intrauterine Growth Retardation
- Author
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Yaotian Fan, Mengzhi Wang, Honghua Jiang, Hao Zhang, Shuang Guo, and Mabrouk Elsabagh
- Subjects
0301 basic medicine ,Arginine ,Intrauterine growth restriction ,Apoptosis ,Endogeny ,Glutamates ,Pregnancy ,Pathology ,Medicine ,RB1-214 ,reproductive and urinary physiology ,Caspase 8 ,Fetal Growth Retardation ,biology ,Caspase 3 ,Reverse Transcriptase Polymerase Chain Reaction ,Cytochrome c ,NF-kappa B ,Cytochromes c ,Caspase 9 ,female genital diseases and pregnancy complications ,Liver ,Female ,Tumor necrosis factor alpha ,Research Article ,medicine.medical_specialty ,congenital, hereditary, and neonatal diseases and abnormalities ,Article Subject ,Blotting, Western ,Immunology ,Radioimmunoassay ,Proinflammatory cytokine ,03 medical and health sciences ,Internal medicine ,In Situ Nick-End Labeling ,Animals ,Sheep ,030109 nutrition & dietetics ,Tumor Necrosis Factor-alpha ,business.industry ,Body Weight ,Cell Biology ,medicine.disease ,030104 developmental biology ,Endocrinology ,Hepatocytes ,Hepatic stellate cell ,biology.protein ,business - Abstract
L-arginine (Arg) is a semiessential amino acid with several physiological functions. N-Carbamylglutamate (NCG) can promote the synthesis of endogenous Arg in mammals. However, the roles of Arg or NCG on hepatic inflammation and apoptosis in suckling lambs suffering from intrauterine growth restriction (IUGR) are still unclear. The current work is aimed at examining the effects of dietary Arg and NCG on inflammatory and hepatocyte apoptosis in IUGR suckling lambs. On day 7 after birth, 48 newborn Hu lambs were selected from a cohort of 432 twin lambs. Normal-birthweight and IUGR Hu lambs were allocated randomly (n=12/group) to control (CON), IUGR, IUGR+1% Arg, or IUGR+0.1% NCG groups. Lambs were fed for 21 days from 7 to 28 days old. Compared with CON lambs, relative protein 53 (P53), apoptosis antigen 1 (Fas), Bcl-2-associated X protein (Bax), caspase-3, cytochrome C, tumor necrosis factor alpha (TNF-α), nuclear factor kappa-B (NF-κB) p65, and NF-κB pp65 protein levels were higher (P<0.05) in liver from IUGR lambs, whereas those in liver from IUGR lambs under Arg or NCG treatment were lower than those in IUGR lambs. These findings indicated that supplementing Arg or NCG reduced the contents of proinflammatory cytokines at the same time when the apoptosis-related pathway was being suppressed, thus suppressing the IUGR-induced apoptosis of hepatic cells.
- Published
- 2020
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34. Anaerobic fermentation of rice bran using rumen liquor for desirable chemical changes as animal feed
- Author
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Khan Islam, Mabrouk Elsabagh, Renlong Lv, Hoang Dang, Toshihisa Sugino, and Taketo Obitsu
- Subjects
General Veterinary ,Animal Science and Zoology - Abstract
Objective: The objectives of this research are to overcome the limitations of rice bran (RB) and de-oiled rice bran (DORB) by fermentation anaerobically using inoculum from the rumen of a canulated sheep for desirable chemical changes. Materials and Methods: Initially, RB and DORB were fermented by 10% rumen liquor for 12 h at 39°C at different moisture levels (10, 20, 30, 40, 50, and 60% phosphate buffer). Again, DORB was fermented for 24, 48, and 72 h at 39°C using 10% rumen liquor at different moisture levels (10, 20, 30, 40, 50, and 60% phosphate buffer). Before and after fermentation, RB and DORB were analyzed for pH, proximate components, neutral detergent fiber (NDF), total-P, inorganic-P, and phytate-P. Results: Fermentation of RB and DORB for 12 h reduced (p < 0.05) pH, crude fiber (CF),NDF, and phytate-P, but increased (p < 0.05) the content of inorganic-P. Subsequent fermentation of DORB for 24, 48, and 72 h reduced pH, CF, and NDF. Total-P of fermented DORB remained similar till 72 h fermentation (p < 0.05). But, inorganic-P increased with the increasing duration (24, 48, and 72 h) of fermentation and increased (30, 40, and 50) moisture level (p < 0.05). Alternatively, phytate-P decreased with increasing duration and moisture level (p < 0.05). Conclusion: Inoculation of rumen microbes and incubation of RB (12 h) and DORB (24 h) at room temperature reduced phytate-P and fiber content (CF and NDF) when the moisture level was up to 50%; those are the indicators to reduce the limitation of RB and DORB to use as feed for non-ruminant animals like poultry and pigs.
- Published
- 2022
35. IMPACT OF SPIRULINA PLATENSIS ALGAE AND VITAMIN A SUPPLEMENTATION TO LATE PREGNANT EWES ON THEIR LAMB’S SURVIVABILITY AND PERFORMANCE
- Author
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Hamada Mahboub, Sameh Ramadan, Mabrouk Attia Abd Eldaim, and Mabrouk Elsabagh
- Subjects
Vitamin ,Creatinine ,Pregnancy ,business.industry ,Domestic sheep reproduction ,Retinol ,Micronutrient ,medicine.disease ,chemistry.chemical_compound ,Animal science ,chemistry ,Medicine ,Spirulina (dietary supplement) ,business ,Intramuscular injection - Abstract
Micronutrient supplementation during late gestation can enhance the metabolic profile and physiological wellbeing of ewes and their lambs. This study was carried out to evaluate the impact of Spirulina platensis (SP) and vitamin A supplementation during late gestation on the pregnant ewes’ health and their newly born lambs’ viability and performance. One month before lambing, thirty six pregnant ewes (47.4±0.52 kg BW) were randomly assigned into three experimental groups (n=6 with 2 replicates for each treatment) and received one of three treatments: intramuscular injection of 1 mL saline per ewe twice a week (control); 1 g/10 kg BW of ewes /day of Spirulina platensis powder (SP) was added to the concentrate mixture; and intramuscular injection of 1 mL vitamin A (50,000 IU) per ewe twice a week. Results revealed that significantly (P< 0.05) decreased in the ewes’ serum alanine aminotransferase (ALT) activity and increased the serum creatinine level of control group after lambing. However, supplementation with SP and vitamin A normalized serum ALT activity and creatinine level after lambing. Also, SP enhanced serum levels of glucose, triacylglycerol and total cholesterol after lambing. In addition, supplementation of late pregnant ewes with SPincreased total leucocytes count and serum vitamin A concentration of their newly born lambs. Furthermore, SP and vitamin A supplementation to the pregnant ewes increased newly born lambs’ birth weights and body temperatures, while they decreased the stillbirth by 56% (11.1%) and 43% (14.3%), respectively compared to those of the control group (25%). Finally, SP and vitamin A ameliorated the lambing-induced stress in ewes and lambs represented by reducing serum levels of tumor necrosis factor alpha in both ewes and their lambs. Thus, supplementation of pregnant ewes with SP and vitamin A improved their health at lambing and enhanced their lambs’ survivability and performance.
- Published
- 2018
36. Assessing the impact ofBacillusstrains mixture probiotic on water quality, growth performance, blood profile and intestinal morphology of Nile tilapia,Oreochromis niloticus
- Author
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Ahmad Hamza, Radi A. Mohamed, Mahmoud A.O. Dawood, Foad Farrag, Mabrouk Elsabagh, Eman M. Moustafa, Olivier Decamp, and Mahmoud Eltholth
- Subjects
0301 basic medicine ,Bacillus (shape) ,biology ,04 agricultural and veterinary sciences ,Aquatic Science ,Intestinal morphology ,biology.organism_classification ,law.invention ,03 medical and health sciences ,Nile tilapia ,Oreochromis ,Probiotic ,030104 developmental biology ,law ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Water quality ,Food science - Published
- 2018
37. Effects of nitrogen fertilizer and harvesting stage on photosynthetic pigments and phytol contents of Italian ryegrass silage
- Author
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Yuzo Kurokawa, Taketo Obitsu, Renlong Lv, Toshihisa Sugino, Kensuke Kawamura, and Mabrouk Elsabagh
- Subjects
Chlorophyll ,0106 biological sciences ,Pheophytin ,Lutein ,Nitrogen ,Silage ,engineering.material ,Biology ,Photosynthesis ,01 natural sciences ,Phytol ,chemistry.chemical_compound ,Animal science ,Lolium ,Fertilizers ,Carotenoid ,chemistry.chemical_classification ,Pheophytins ,0402 animal and dairy science ,food and beverages ,Pigments, Biological ,04 agricultural and veterinary sciences ,General Medicine ,beta Carotene ,040201 dairy & animal science ,chemistry ,Agronomy ,engineering ,Fertilizer ,General Agricultural and Biological Sciences ,010606 plant biology & botany - Abstract
This study aimed to investigate the effects of nitrogen (N) fertilizer application and harvesting stage on the contents of chlorophyll, phytol and carotenoids (β-carotene and lutein) in Italian ryegrass herbage before and after ensiling, and the extent of phytol preservation after ensiling. Three rates of N fertilizer (0, 60 and 120 kg N/ha) were applied by top-dressing as an additional fertilizer. The herbage harvested at booting stage (27 weeks of age) or heading stage (29 weeks of age) were wilted for 1 day, then ensiled for 60 days using a small-scale pouch system. In the pre-ensiled herbages, increasing N fertilizer application increased the contents of crude protein and photosynthetic pigments, and these contents were also higher at the booting stage compared with the heading stage. In the silage, increasing N fertilizer application also increased the contents of crude protein, the photosynthetic pigments and their derivatives (pheophytin and pheophorbide), while harvesting stage did not affect the contents of β-carotene, chlorophylls or pheophorbide. Nitrogen fertilizer application and early harvesting of herbage increased lutein and phytol contents in Italian ryegrass silage. Lutein and phytol in Italian ryegrass herbage are indicated to be well preserved during ensiling.
- Published
- 2017
38. Changes in the growth, humoral and mucosal immune responses following β-glucan and vitamin C administration in red sea bream, Pagrus major
- Author
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Mabrouk Elsabagh, Mahmoud A.O. Dawood, Md. Masum Billah, Mohamed M. Zayed, Shunsuke Koshio, Amr I. Zaineldin, and Amira A. Omar
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,Protein efficiency ratio ,biology ,Vitamin C ,Triglyceride ,04 agricultural and veterinary sciences ,Aquatic Science ,biology.organism_classification ,Malondialdehyde ,Pagrus major ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Animal science ,Immune system ,chemistry ,Biochemistry ,040102 fisheries ,medicine ,0401 agriculture, forestry, and fisheries ,medicine.symptom ,Weight gain ,Glucan - Abstract
In order to study the effects of dietary β-glucan (BG), vitamin C (VC) and their combination (BG/VC) on growth, humoral and mucosal immune responses of red sea bream (Pagrus major) and its resistance to low water salinity stress, 240 healthy fish with initial body weight of 1.98 ± 0.01 g were randomly divided into four groups: a control group fed with basal diet and three treated groups fed with basal diets supplemented with 1000 mg kg− 1 BG, 800 mg kg− 1 VC, and the combination of 500 mg kg− 1 BG + 400 mg kg− 1 VC, respectively. The obtained results showed that, compared with the control, the final body weight, weight gain and specific growth rate, feed and protein efficiency ratio, body protein content, nitro blue tetrazolium (NBT), total serum protein and tolerance against low salinity stress increased in fish fed BG or/and VC supplementations with the highest being in fish fed both BG and VC supplements while body moisture content, plasma glucose, triglyceride and malondialdehyde (MDA) contents decreased (P Statement of relevance A combination of β-glucan and vitamin C presented an additive effect on inducing the growth, immune responses and oxidative status of red sea bream. We recommend that treatment of 500 mg of β-glucan and 400 mg of vitamin C per kg diet is the appropriate concentration of inducing the red sea bream immune response.
- Published
- 2017
39. Nutritional quality of maize in response to drought stress during grain-filling stages in mediterranean climate condition
- Author
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Shohidul Islam, Tulin Sahin, A. El-Sabagh, Celaleddin Barutçular, Mabrouk Elsabagh, Halef Dizlek, and Çukurova Üniversitesi
- Subjects
0106 biological sciences ,Mediterranean climate ,Drought stress ,Grain filling stage ,General Veterinary ,Agroforestry ,food and beverages ,Starch ,04 agricultural and veterinary sciences ,Nutritional quality ,Grain filling ,Biology ,Zea mays ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Oil and protein content ,Agronomy ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Deficit irrigation ,General Agricultural and Biological Sciences ,010606 plant biology & botany - Abstract
WOS: 000389137600006 Maize is considered one of the most essential dietary components in human food and animal feeding. The objectives of the present study were to quantify the effects of drought stress on qualitative traits of maize at grain-filling stages. Hybrids maize seeds were grown by applying full and water stress conditions during the grain filling stage. Various nutritional properties (crude oil, starch, grain protein content) were determined in 2014 and 2015 at the second crop growing season in Adana, Turkey. Based on the results of this study, genotype and environment were found to influence all quality traits significantly. Further, result of study suggest that water stress caused a significant reduction in major quality traits. Grain weight and grain quality yield as well crude oil, protein and ash yield were significantly decreased due to water deficit condition in the both growing seasons. Significant differences were observed among hybrids in respect of all measurements due to irrigation regimes. The genotypes, Sancia and Calgary were tolerant by producing higher grain weight. Accordingly, grain qualities of 71May69, Aaccel and Calgary maize hybrids were less affected under drought stress.
- Published
- 2016
40. Exposure to blue LED light before the onset of darkness under a long-day photoperiod alters melatonin secretion, feeding behaviour and growth in female dairy calves
- Author
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Watanabe Takashi, Shiro Kushibiki, Taketo Obitsu, Yui Takao, Mabrouk Elsabagh, Akiko Shinoda, Toshihisa Sugino, and Mon Mamiko
- Subjects
Light ,Color ,Biology ,Body weight ,Long day ,Melatonin ,03 medical and health sciences ,Animal science ,medicine ,Animals ,Melatonin secretion ,030304 developmental biology ,Blue light ,photoperiodism ,0303 health sciences ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,Feeding Behavior ,Darkness ,040201 dairy & animal science ,Circadian Rhythm ,Hay ,Cattle ,Female ,General Agricultural and Biological Sciences ,medicine.drug - Abstract
The effect of blue LED on melatonin secretion, feeding behaviour and growth was addressed in Holstein female dairy calves. In Exp.1, six animals (8 weeks old, 97 ± 4.1 kg BW) were exposed to yellow or blue LED for 2 hr before darkness over 7 days under a long-day photoperiod (LDPP). In Exp. 2, six animals (8 weeks old, 88.5 ± 4.8 kg BW) were exposed to blue light from a white LED all daytime or a yellow LED for 2 hr before the darkness of LDPP (blue light cut) over 3 weeks. In Exp. 1, blue light mildly suppressed melatonin secretion during the 2-hr treatment but did not affect the timing of the nightly melatonin rise. However, the rise in nighty melatonin levels was higher with yellow than blue LED. In Exp. 2, white LED completely suppressed melatonin secretion during the 2-hr treatment, but plasma melatonin concentrations were similar during the darkness. Grass hay intake, rumination time, frequency of water intake and body weight gain were higher in animals exposed to the yellow rather than the white LED. Overall results indicate that exposure to blue light from white LEDs under an LDPP suppresses melatonin secretion and might negatively impact the development of female dairy calves.
- Published
- 2019
41. Effects of direct-fed microbial supplementation on growth performance and metabolic profile of newborn lambs weaned at different ages
- Author
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Ibrahim A. Alhidary, Riyadh S. Aljumaah, Abdullah N. Alowaimer, Mabrouk Elsabagh, Mutassim M. Abdelrahman, and Ramzi A. Amran
- Subjects
Oral dose ,Creatinine ,General Veterinary ,Triglyceride ,business.industry ,animal diseases ,Mortality rate ,respiratory system ,Body weight ,chemistry.chemical_compound ,Animal science ,chemistry ,parasitic diseases ,Medicine ,Weaning ,Animal Science and Zoology ,business ,Metabolic profile ,Serum cortisol - Abstract
The aim of this study was to investigate the effects of direct fed microbial (DFM) supplementation on growth performance, metabolic profile and mortality rate of newborn lambs at different weaning ages. Five days old sixty Najdi male lambs (5.40 ± 0.10 kg BW) were randomly assigned to 1 of 4 treatments (15 lambs each): lambs received either no DFM and weaned at 60 days of age (control) or oral dose of DFM (5 ml) at 5, 10 and 15 days old, and weaned at 30 (DFM30), 45 (DFM45) or 60 (DFM60) days old, respectively. Final body weight was higher (P=0.05) and tended to be higher (P=0.09) in DFM30 and DFM45 lambs, respectively whereas body weight gain was higher (P=0.01) in DFM45 and DFM60 lambs. The mortality rate was declined from 33.3s% to 6.67% in DFM30 and DFM45 lambs compared to control ones. Serum cortisol and creatinine levels tended to be reduced (P=0.07 and 0.11, respectively) in DFM30 and DFM45 lambs. The serum total cholesterol was highest (P=0.02) in DFM60 lambs and lowest in both DFM45 and control lambs. Similarly, the DFM30 and DFM45 lambs showed a declined serum triglyceride compared with control and DFM60 lambs (P=0.02). Serum phosphorus declined (P=0.04) in DFM30 treated lambs while serum zinc and copper levels decreased (P=0.02 and 0.05, respectively) in all treatments compared to control lambs. These results indicated that DFM supplementation may enhance the growth and health of early weaned lambs.
- Published
- 2019
42. An evaluation of dietary selenium nanoparticles for red sea bream (Pagrus major) aquaculture: growth, tissue bioaccumulation, and antioxidative responses
- Author
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Shunsuke Koshio, Amr I. Zaineldin, Hamada A. Ahmed, Mabrouk Elsabagh, Mahmoud A.O. Dawood, Hien Van Doan, and Mohamed M. Abdel-Daim
- Subjects
Fish Proteins ,Antioxidant ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,chemistry.chemical_element ,Aquaculture ,010501 environmental sciences ,01 natural sciences ,Feed conversion ratio ,Antioxidants ,Pagrus major ,chemistry.chemical_compound ,Selenium ,Animal science ,medicine ,Environmental Chemistry ,Animals ,Tissue Distribution ,0105 earth and related environmental sciences ,biology ,Triglyceride ,Chemistry ,Cholesterol ,General Medicine ,biology.organism_classification ,Pollution ,Animal Feed ,Bioaccumulation ,Sea Bream ,Hematocrit ,Dietary Supplements ,Nanoparticles ,Composition (visual arts) ,Peptide Hydrolases - Abstract
Selenium nanoparticles (Se-NPs) were added at 0, 0.5, 1, and 2 mg per kg diet to assess its effects on the performance, Se bioaccumulation, blood health, and antioxidant status of red sea bream. After 45 days, Se-NPs positively impacted the growth and feed efficiency of red sea bream especially by 1 mg per kg diet. No significant (P > 0.05) changes in survival and somatic indices were noticed among groups. Dietary Se-NPs significantly (P < 0.05) increased the protein, lipid, and Se contents in the whole body, muscle, and liver tissues, whereas decreasing the whole-body moisture content of treated groups compared with the Se-NP-free group. Using of Se-NPs at 2 mg per kg diet resulted in the highest Se content in the complete body, muscle, and liver. Significantly enhanced intestine protease activity and hematocrit levels accompanied with low cholesterol and triglyceride were observed in fish fed Se-NP-enriched diets. Fish fed on Se-NPs at 0.5, 1, and 2 mg Se-NPs per kg diet exhibited significantly higher values of biological antioxidant potential than the control group (P < 0.05). Therefore, the obtained results recommends adding 1 mg Se-NPs per kg diet to improve the growth, feed efficiency, blood health, and antioxidant defense system of red sea bream.
- Published
- 2019
43. Effect of varying fermentation conditions with ensiling period and inoculum on photosynthetic pigments and phytol content in Italian ryegrass (Lolium multiflorum Lam.) silage
- Author
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Kensuke Kawamura, Mabrouk Elsabagh, Taketo Obitsu, Toshihisa Sugino, Renlong Lv, and Yuzo Kurokawa
- Subjects
Chlorophyll ,Lutein ,Time Factors ,Silage ,chemistry.chemical_compound ,Phytol ,Animal science ,Lactobacillales ,Lolium ,Lactic Acid ,Photosynthesis ,Carotenoid ,chemistry.chemical_classification ,biology ,Chemistry ,food and beverages ,General Medicine ,Lolium multiflorum ,Hydrogen-Ion Concentration ,biology.organism_classification ,Carotenoids ,Lactic acid ,Fermentation ,sense organs ,General Agricultural and Biological Sciences - Abstract
This study aimed to investigate the effect of an ensiling period (Experiment 1) and adding lactic acid bacteria (LAB, Experiment 2) on the changes in carotenoid, chlorophyll, and phytol in ensiled Italian ryegrass (IR, Lolium multiflorum Lam.). In Experiment 1, the IR herbage ensiled into plastic bags was analyzed for the contents of photosynthetic pigments and phytol over a 5-week period. During the ensiling process, the β-carotene content decreased (p < .05), whereas the lutein content did not change. Although the chlorophyll content decreased (p < .05) after ensiling, the phytol content barely changed until week 5. In Experiment 2, IR herbage was ensiled without additive, as a Control, or with LAB for 60 days. The pH was lower (p < .05) and lactic acid content was higher (p < .05) for the LAB silage than for the Control. The chlorophyll content in silage was not affected by the LAB; however, the β-carotene content was higher (p < .05) for the LAB silage than for the Control. Phytol and lutein contents in the herbage did not change after ensiling. These results indicate that phytol and lutein in IR herbage can be preserved well in silage, irrespective of their fermentation condition.
- Published
- 2019
44. YIELD AND NUTRITIVE VALUE OF ITALIAN RYEGRASS (LOLIUM ITALICUM L.) IS INFLUENCED BY DIFFERENT LEVELS OF NITROGENOUS FERTILIZER
- Author
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Akdeniz, Hakki, Bozkurt, Mehmet Ali, Hosaflioglu, Ibrahim, Islam, Mohammad Sohidul, Hossain, Akbar, Mabrouk Elsabagh, Iqbal, Muhammad Aamir, and El Sabagh, Ayman
- Abstract
Italian ryegrass is often fertilized with high levels of nitrogen (N) in order to ensure a maximum biomass yield. While overdose of any fertilizer is neither cost-effective nor environmentally sound. In this context, the study was carried out at the agricultural experimental area of Igdir University during 2013, to find out the optimum dose of nitrogenous fertilizers for maximizing the biomass yield as well as nutrients content of Italian ryegrass. Treatments were five levels of nitrogen viz., 0 (N-o), 2 (N-2), 4 (N-4), 6 (N-6) or 8 (N-8) kg da(-1), respectively. Treatments were arranged in a randomized completed block design with three replications. Data on green herbage and dry biomass yield as well as nutrients content such as Calcium (Ca), Magnesium (Mg), potassium (K), phosphorus (P), Crude protein (CP), Crude ash and Tetany ratio (K/(Ca+Mg)) were examined under the different levels of N. Results of the study showed that increasing rate of N fertilizer increased the green herbage as well as dry biomass yield of Italian ryegrass. The highest green herbage and dry biomass yield were recorded for the 8 kg N da(-1) (N-8) (1142.7 and 473.7 kg da(-1)) treated grass followed by those treated with a 6 kg N da(-1) (N-6) (1070.2 and 444.8 kg da(-1)). Similarly, the maximum seed yield was also recorded in the treatment of 8 kg N da(-1), followed by the treatment of 6 kg N da(-1). Considering the nutritional values, the levels of N and K in the ryegrass were decreased from the initial growth stages to seed stages. While, CP, Mg, P and Tetany ratios were decreased as growth stages of plant increased. Therefore, the application of 6 to 8 kg N da(-1) could be recommended for higher green herbage, dry biomass yield and also improving the nutrients content of Italian ryegrass under the condition of Igdir-Turkey.
- Published
- 2019
45. Probiotics as an environment-friendly approach to enhance red sea bream, Pagrus major growth, immune response and oxidative status
- Author
-
M. Ángeles Esteban, Manabu Ishikawa, Mabrouk Elsabagh, Mahmoud A.O. Dawood, Shunsuke Koshio, and Amr I. Zaineldin
- Subjects
0301 basic medicine ,medicine.medical_treatment ,Aquatic Science ,Gut flora ,law.invention ,Microbiology ,Pagrus major ,Superoxide dismutase ,Random Allocation ,03 medical and health sciences ,Probiotic ,Immune system ,Aquaculture ,Lactobacillus rhamnosus ,law ,medicine ,Animals ,Environmental Chemistry ,Food science ,Protease ,biology ,Lacticaseibacillus rhamnosus ,business.industry ,Probiotics ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Animal Feed ,Immunity, Innate ,Diet ,Gastrointestinal Microbiome ,Perciformes ,Oxidative Stress ,030104 developmental biology ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,business - Abstract
A usual strategy in modern aquaculture to combat production bottlenecks associated with intensification is preventive health care through the use of consumer and environment-friendly alternatives including probiotics. The current study evaluates the influence of Lactobacillus rhamnosus (LR), a lyophilized probiotic bacterium, on health status and performance of red sea bream (Pagrus major). Probiotics were incorporated in the diets at four different concentrations: 0 (control diet, LR0), 10(2) (LR1), 10(4) (LR2) and 10(6) (LR3) cells g(-1) and diets were administered to the fish for a period of 8 weeks. After the feeding trial, final body weight, body weight gain, specific growth rate, protease activity, protein digestibility, Lactobacillus sp. intestinal count, and superoxide dismutase were significantly higher in all probiotic-fed groups (P 0.05). In addition, lipid and dry matter digestibility, reactive oxygen metabolites, biological antioxidant potential, and humoral and mucosal immune parameters including (total serum protein, alternative complement pathway, bactericidal and peroxidase activities) were also significantly elevated in fish fed probiotic supplementations being the effects dose-dependent. All growth, feed utilization, immune and oxidative parameters were significantly improved following probiotic administration. Present results revealed that L. rhamnosus is a promising probiotic candidate employed to help red sea bream protect themselves, thus promoting safe farming that would be less dependent on chemotherapy against infectious diseases.
- Published
- 2016
46. Physiological response, blood chemistry profile and mucus secretion of red sea bream (Pagrus major) fed diets supplemented with Lactobacillus rhamnosus under low salinity stress
- Author
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Saichiro Yokoyama, Mabrouk Elsabagh, Manabu Ishikawa, Adissin Olivier, Shunsuke Koshio, Zhang Yukun, Weilong Wang, and Mahmoud A.O. Dawood
- Subjects
Blood Glucose ,Fish Proteins ,0301 basic medicine ,Salinity ,Hydrocortisone ,Physiology ,Bilirubin ,Aquatic Science ,Biochemistry ,Blood Urea Nitrogen ,Microbiology ,Pagrus major ,03 medical and health sciences ,chemistry.chemical_compound ,Animal science ,Immune system ,Lactobacillus rhamnosus ,Stress, Physiological ,Animals ,Triglycerides ,Peroxidase ,biology ,Triglyceride ,Lacticaseibacillus rhamnosus ,Probiotics ,Blood Proteins ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Mucus ,Sea Bream ,Cholesterol ,030104 developmental biology ,chemistry ,Blood chemistry ,Myeloperoxidase ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries - Abstract
Environmental stressors caused by inadequate aquaculture management strategies suppress the immune response of fish and make them more susceptible to diseases. Therefore, efforts have been made to relieve stress in fish by using various functional feed additives in the diet, including probiotics. The present work evaluates the effects of Lactobacillus rhamnosus (LR) on physiological stress response, blood chemistry and mucus secretion of red sea bream (Pagrus major) under low salinity stress. Fish were fed four diets supplemented with LR at [0 (LR0), 1 × 102 (LR1), 1 × 104 (LR2) and 1 × 106 (LR3) cells g−1] for 56 days. Before stress, blood cortisol, urea nitrogen (BUN) and total bilirubin (T-BIL) showed no significant difference (P > 0.05), whereas plasma glucose and triglyceride (TG) of fish-fed LR2 and LR3 diets were significantly lower (P 0.05). In addition, the fish that received LR-supplemented diets showed significantly higher tolerance against low salinity stress than the fish-fed LR-free diet (P
- Published
- 2016
47. Metabolomic profiling reveals differential effects of glucagon-like peptide-1 and insulin on nutrient partitioning in ovine liver
- Author
-
Dai Taniguchi, Toshihisa Sugino, Taketo Obitsu, and Mabrouk Elsabagh
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Insulin ,medicine.medical_treatment ,General Medicine ,Metabolism ,Biology ,Glucagon-like peptide-1 ,03 medical and health sciences ,030104 developmental biology ,Endocrinology ,Gluconeogenesis ,Internal medicine ,Lipogenesis ,Ketogenesis ,medicine ,Glycolysis ,General Agricultural and Biological Sciences ,Hormone - Abstract
This study was conducted to identify the insulin-independent actions of glucagon-like peptide-1 (GLP-1 (7-36 amide)) in partitioning nutrient metabolism in ovine liver. Four Suffolk wethers (60.0 ± 6.7 kg body weight (BW)) were used in a repeated-measure design under euglycemic--hyperinsulinemic and hyper -GLP-1 clamps for 150 min with intravenous infusion of insulin (0.5 mU/kg BW/min; from 0 to 90 min), GLP-1 (0.5 µg/kg BW/min; from 60 to 150 min) and both hormones co-administered from 60 to 90 min. Liver biopsies were collected at 0, 60, 90 and 150 min to represent the metabolomic profiling of baseline, insulin, insulin plus GLP-1, and GLP-1, respectively, and were analyzed for metabolites using Capillary Electrophoresis Time-of-Flight Mass Spectrometer. Metabolomics analysis reveals 51 metabolites as being significantly altered (P
- Published
- 2016
48. Postruminal supply of amino acids enhances ghrelin secretion and lipid metabolism in feed-deprived sheep
- Author
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Taketo Obitsu, Atsushi Haruno, Takeshi Fujieda, Makoto Miura, Motomi Ishikake, Yasuaki Sakamoto, Toshihisa Sugino, and Mabrouk Elsabagh
- Subjects
0301 basic medicine ,Blood Glucose ,Male ,medicine.medical_specialty ,Duodenum ,medicine.medical_treatment ,Glucagon ,03 medical and health sciences ,NEFA ,Valine ,Internal medicine ,medicine ,Animals ,Insulin ,Amino Acids ,Insulin-Like Growth Factor I ,chemistry.chemical_classification ,Sheep ,Chemistry ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,Lipid Metabolism ,040201 dairy & animal science ,Animal Feed ,Ghrelin ,Amino acid ,Glutamine ,030104 developmental biology ,Endocrinology ,General Agricultural and Biological Sciences ,Food Deprivation ,Ghrelin secretion - Abstract
Single amino acids (AA) feeding is gaining more attention for their functional roles beyond nutritional needs. This study aimed to describe the endocrine and metabolic responses to a single AA administration (at 10% of MP for maintenance) in 48 hr fasted sheep (n = 4) receiving, over continued 4 hr, a duodenal infusate of saline (control), glutamate (Glu), glutamine (Gln), lysine HCl (Lys), threonine (Thr), or valine (Val) in a 4 by 6 Youden square design with weekly intervals. Blood samples were collected at 0, 15, 30, 60, 120, 180, and 240 min relative to the infusion onset, and plasma AA, glucose, β-hydroxybutyric acid (BHBA), nonesterified fatty acids (NEFA), ghrelin, insulin, glucagon, and insulin-like growth factor 1 (IGF-1) concentrations were measured. The results showed that the duodenal supply of Glu, Gln, Lys, Thr, and Val enhanced ghrelin release. Administration of Glu, Gln, and Val declined plasma BHBA concentrations, whereas plasma NEFA levels were decreased with Gln and Thr. Insulin concentration was greater with Thr, glucagon levels were increased with Lys, Thr, Val, and Glu, whereas IGF-1 levels were enhanced with Gln, Lys, and Thr supply. Thus, selective AA feeding can positively adjust the endocrine status and counteract the feed restriction-induced lipid mobilization.
- Published
- 2018
49. Circadian feeding schedules in gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax): A comparative approach towards improving dietary fish oil utilization and n-3 LC-PUFA metabolism
- Author
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Orhan Tufan Eroldoğan, Durali Eraslan, Yılmaz Emre, Ece Evliyaoğlu, Giovanni M. Turchini, Hatice Asuman Yilmaz, Mabrouk Elsabagh, Nesrin Emre, and Çukurova Üniversitesi
- Subjects
0301 basic medicine ,Dietary lipid ,Canola oil ,Aquatic Science ,Fish oil ,Feed conversion ratio ,03 medical and health sciences ,Animal science ,Aquaculture ,Clock gene ,Circadian rhythms ,Sea bass ,European sea bass ,chemistry.chemical_classification ,Meal ,biology ,business.industry ,Aquafeed ,04 agricultural and veterinary sciences ,biology.organism_classification ,Fatty acid ,030104 developmental biology ,chemistry ,Gilthead sea bream ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Mixed feeding schedule ,Dicentrarchus ,business ,Polyunsaturated fatty acid - Abstract
The objective of this study was to test the potential the alternation of fish oil- and canola oil- based diets offered in a circadian alternating schedule, in gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), two commercially important marine species in Mediterranean aquaculture. The two species were kept separately, juvenile European sea bass and gilthead sea bream were randomly distributed each into 4 triplicate groups at 40 fish per tank (n = 3, N = 12; per species). Two experimental extruded diets differing only in the added dietary lipid sources, either 100% fish oil (FO-D) or 100% canola oil (CO-D), but having the same formulation, were manufactured. The experimental feeding schedules adopted were: CO-D in the first meal with FO-D in the second meal (COam), and FO-D in first meal with CO-D in the second meal (COpm), and two control treatments: a positive control treatment, fed FO-D in both meals (posCT), and a negative control treatment, fed CO-D in both meals (negCT). Test diets were fed twice a day at a 2% body weight per day for 60 days. Gilthead sea bream and European sea bass fed the negCT diets exhibited the poorest performance. The COpm diet worked while the COam diet tended to work equally well as the fish oil diet (posCT) in terms of fish performances and feed efficiency in gilthead sea bream but the posCT diet tended to outperform both COam and COpm diets in European sea bass. Important differences in the whole body nutrient composition were recorded across treatments, as well as in their fatty acid composition. The whole-body contents of n-3 polyunsaturated fatty acids and in particular DHA, but not EPA, were highest with COpm diets followed by COam, posCT and negCT diets, in gilthead sea bream, while were highest with posCT and lowest with negCT diets with a similar response with CO-am and CO-pm diets in European sea bass. This study showed that the daily alternation of fish oil- and canola oil- based diets in comparison to the continuous feeding with either fish oil- or canola oil- based diets can enhance growth performance and feed intake and can promote EPA and DHA content in the fatty acid profile of gilthead sea bream. Clear species-specific responses were also observed where the effect of alternate oil feeding on growth performance and feed efficiency as well as on fatty acid profile was less marked in European sea bass. Mixed feeding schedules, based on at hoc circadian patterns, may be instrumental to optimise fish oil use in aquafeeds, maintain the targeted n-3 fatty acids in fish tissues and ultimately support the further growth of an environmental sustainable and economically viable aquaculture industry. © 2018 Elsevier B.V.
- Published
- 2018
50. Effects of fat-enriched diet and methionine on insulin sensitivity in lactating cows1
- Author
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Naoko Moriya, Yoshihisa Hasegawa, K. Yamaji, Yoshikazu Nagao, Taketo Obitsu, H. Kobayashi, Hiroyuki Shingu, Kohzo Taniguchi, Mabrouk Elsabagh, Kenji Kangawa, Shiro Kushibiki, Rika Fukumori, Masayasu Kojima, and Toshihisa Sugino
- Subjects
Methionine ,Cholesterol ,Insulin ,medicine.medical_treatment ,Phospholipid ,Lipid metabolism ,General Medicine ,chemistry.chemical_compound ,Animal science ,chemistry ,Latin square ,Jugular vein ,Genetics ,medicine ,Animal Science and Zoology ,Steady state (chemistry) ,Food Science - Abstract
The hyperinsulinemic-euglycemic clamp (EGC) technique was used to investigate the effects of calcium salts of long-chain fatty acids (LCFA-Ca) and rumen-protected Met (RPM) on insulin sensitivity in the peripheral tissues of lactating cows. Six multiparous Holstein cows were used in a 3 × 3 Latin square experiment in each 14-d period. Dietary treatments were 0 (RPM0), 20 (RPM20), and 60 (RPM60) g/d of RPM, supplemented with a diet containing 1.5% of LCFA-Ca equal to 110% of the cows' ME requirement. And as a control for the 3 LCFA-Ca-containing diets, a dietary treatment without LCFA-Ca (Con) was also included. After a 10-d adaptation period, milk samples were collected for 4 d, and EGC experiments were performed on d 14 of each treatment period. Insulin solution was infused through a jugular vein catheter at a rate of 0.1, 0.2, 0.3, and 0.4 milliunits·kg BW-1·min-1 for 30 min and then at a rate of 0.5 milliunits·kg BW-1·min-1 for 60 min. Glucose solution was variably infused to maintain plasma glucose at steady state through the same catheter. Blood samples for measurements were taken using the contralateral catheter. Plasma total cholesterol, cholesterol ester, free cholesterol, and phospholipid concentrations in RPM0 and RPM20 were higher than those in Con, whereas the concentrations in RPM60 were low at the same degree of those in RPM0 (P < 0.05). Plasma Met concentration was greatest in RPM60 (P < 0.05). In the EGC experiment, the glucose infusion rate was greater in RPM60 than in RPM0 and RPM20 and an effective concentration of insulin resulting in 50% maximal glucose infusion rate was lower in RPM60 compared with RPM0 (P < 0.05), indicating that insulin sensitivity was intensified in RPM60. Although the insulin sensitivity evaluated from the EGC data in RPM0, RPM20, and RPM60 was not different from Con, a slight decline was observed in RPM0 and insulin sensitivity in RPM60 was higher than Con. Our results from the EGC experiment demonstrated that the feeding RPM lead to increased insulin sensitivity, which suggests that dietary Met affects lipid metabolism via insulin action in lactating dairy cows fed a LCFA-Ca-containing diet.
- Published
- 2015
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