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A new species of the genus Neopheosia Matsumura, 1920, N. pseudofasciata sp. nov. is described and illustrated from Fujian province. The species resembles N. fasciata (Moore, 1888) but differs in a forewing pattern in M3 cell; diagnostic differences are observed in genitalia characters as well. DNA barcode data of the new species and its relatives are deposited in GenBank. A key to the Neopheosia species based on morphology is presented. The holotype is deposited in the Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou.

BackgroundUniversal COVID-19 vaccination programs are now recommended in several countries and represent the most effective preventive measure against COVID-19. However, some reports suggest that vaccination may cause infertility or have adverse effects on pregnancy. Conflicting reports have led to vaccine hesitancy in women planning pregnancy.PurposeTo determine whether vaccination against COVID-19 affects in vitro fertilization (IVF) outcomes, we conducted a meta-analysis.MethodA systematic search was conducted using PubMed, Embase, MEDLINE, and Web of Science databases for all published literature on COVID-19 vaccines and outcomes of IVF. International Prospective Register of Systematic Reviews registration was completed on September 13, 2022 (CRD42022359771).ResultsWe analyzed 20 studies totaling 18,877 individual cases undergoing IVF. COVID-19 vaccination had significant effect on clinical and ongoing pregnancy rate (risk ratio (RR): 0.97; 95% confidence interval (CI): 0.94–0.99; RR: 0.93; 95% CI: 0.87–0.99). These outcomes did not differ between vaccinated and unvaccinated individuals: biochemical pregnancy rate (RR: 0.95; 95% CI: 0.88–1.03), implantation rate (RR: 1.02; 95%CI: 0.97–1.07; P = 0.41), the number of oocytes (mean difference (MD): 0.12; 95% CI: −0.65–0.88) and MII/mature oocytes recovered (MD: 0.27; 95% CI: −0.36–0.90), blastocysts rate (MD: 0.01; 95% CI: −0.04, 0.06), and fertilization rate (MD: 1.08; 95% CI: −0.57, 2.73).ConclusionOur findings suggest that vaccination against COVID-19 does not adversely affect the biochemical pregnancy rates; number of oocytes and MII/mature oocytes obtained; implantation, blastocysts; and fertilization rates in women undergoing IVF treatment. Subgroup analysis showed that the mRNA vaccine had no statistical significance on all indexes (clinical, biochemical, or ongoing pregnancy rates; implantation, blastocysts, or fertilization rates; and the number of oocytes and MII/mature oocytes). The findings of this meta-analysis are anticipated to increase the willingness of women planning IVF treatment to receive COVID-19 vaccination and provide evidence-based medical guidance for the development and implementation of guidelines.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42022359771.

Xiao-Min Zhang, Ming-Hui Lun, Wei Du, Fang Ma, Ze-Qing Huang Department of Anesthesiology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institution, NO.44 Xiaoheyan Road, Dadong District, Shenyang, Liaoning Province, 110042, People’s Republic of ChinaCorrespondence: Xiao-Min Zhang; Ze-Qing Huang Department of Anesthesiology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institution, No.44 Xiaoheyan Road, Dadong District, Shenyang, Liaoning Province, 110042, People’s Republic of China Email zhangxiaomin.99@163.com; huangzeqing0@163.comObjective: To observe the ameliorative effect of kappa opioid receptor (KOR) agonist on rats with neuropathic pain (NP) and investigate the mechanism of action of the calcium ion (Ca2+)/calcium/calmodulin-dependent protein kinase II (CaMKII)/cyclic AMP response element-binding protein (CREB) pathway.Methods: A total of 40 Sprague Dawley rats were randomly divided into four groups: sham-operation group (Sham group), NP model group (NP group), NP + KOR agonist U50488H group (NU group) and NP + specific CaMKII antagonist (KN93) + U50488H group (NKU group). The thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) of each group of rats were determined. ELISA was applied to examine the changes in inflammatory factors and oxidative stress factors, and the apoptotic rate in dorsal root ganglia was observed using TUNEL staining. Ca2+ concentration, content of oxidative stress index ROS and the release of calcitonin gene-related peptide (CGRP) and N-methyl-D-aspartate receptor (NMDAR) in the dorsal root ganglia were measured by the immunofluorescence assay. Finally, Western blotting was performed to detect expression changes in the Ca2+/CaMKII/CREB pathway.Results: The KOR agonist U50488H could improve the values of TWL and MWT of NP the rats, inhibit inflammatory responses and relieve oxidative stress injury. Its mechanisms of action were associated with U50488H repression of Ca2+ influx, reduction of CGRP and NMDAR releases in the dorsal root ganglia and decreases in CaMKII and CREB phosphorylations in NP rats.Conclusion: The KOR agonist ameliorates NP through suppressing the activity of the Ca2+/CaMKII/CREB pathway.Keywords: KORs, neuropathic pain, Ca2+, CaMKII, CREB

The aggregation behavior of hydrogen (H) isotopes after low-energy plasma irradiation is particularly important for tungsten (W) in future fusion devices. Here, using the first-principles calculation, we demonstrated an interesting strain effect that may drive the planar aggregation of interstitial H atoms in W. Although there are attractive interactions between H atoms, the spontaneous nucleation of these platelet-like H clusters in strain-free W appears impossible even at high H concentrations (up to 0.1 at.%) because of the extremely low configurational entropy. However, applied uniaxial strain significantly increased the binding energy of H platelets and enabled planar H clusters to form. These results suggest that uniaxial strain enhances the nucleation and growth of H platelets in W, regardless of whether it is a compressive or tensile strain. Moreover, the binding energy of one-layer H clusters was lower than that of parallel H platelets, implying that the formation of multi-layer H clusters in W and their stability is also promoted by uniaxial strain. Meanwhile, the presence of planar H clusters dramatically reduced the vacancy formation energy in W, which in turn provided an extra trapping site to accommodate excessive H atoms. These results provide an important reference for understanding the H evolution in W-PFMs.

Prompt tuning learns soft prompts to condition frozen Pre-trained Language Models (PLMs) for performing downstream tasks in a parameter-efficient manner. While prompt tuning has gradually reached the performance level of fine-tuning as the model scale increases, there is still a large performance gap between prompt tuning and fine-tuning for models of moderate and small scales (typically less than 11B parameters). In this paper, we empirically show that the trained prompt tokens can have a negative impact on a downstream task and thus degrade its performance. To bridge the gap, we propose a novel Prompt tuning model with an eXtremely small scale (XPrompt) under the regime of lottery tickets hypothesis. Specifically, XPrompt eliminates the negative prompt tokens at different granularity levels through a hierarchical structured pruning, yielding a more parameter-efficient prompt yet with a competitive performance. Comprehensive experiments are carried out on SuperGLUE tasks, and the extensive results indicate that XPrompt is able to close the performance gap at smaller model scales., 15 pages, accepted to EMNLP 2022 main conference

Escherichia coli is a leading cause of bovine mastitis worldwide. The bacteria can rapidly grow in milk and elicit a strong lipopolysaccharide (LPS)/toll-like receptor-4 (TLR4)-dependent inflammatory response. Recently, the long polar fimbriae (LPF) were identified as a promising virulence factor candidate widely distributed in mammary pathogenic E. coli (MPEC) strains. Mammary pathogenic E. coli possess 2 lpf loci encoding LPF1 and LPF2, respectively. By deleting the major fimbrial subunit gene, lpfA, we found that both LPF1 and LPF2 contribute to MPEC adhesion, invasion, and biofilm formation in vitro. The lpf1A and lpf2A mutants showed reduced cytotoxicity in our in vitro cell infection model. Furthermore, we observed that LPF2 induced a mild TLR4-independent proinflammatory response. The median lethal dose (LD50) of both ∆lpf2A and ∆lpf1A∆lpf2A mutants to BALB/c mice increased by 0.38 and 0.15 logs, respectively, whereas that of wild-type strain MPJS13 was 8.69 logs. In contrast, LPF1 deficiency significantly enhanced the LPS/TLR4-mediated inflammatory response in mammary epithelial cells, and the LD50 of the mutant decreased to 8.18 logs. In conclusion, our data suggested that LPF are important in MPEC colonization of mammary cells and may provide a benefit to bacterial intracellular survival that induces persistent bovine mastitis.

Structural bias has recently been exploited for aspect sentiment triplet extraction (ASTE) and led to improved performance. On the other hand, it is recognized that explicitly incorporating structural bias would have a negative impact on efficiency, whereas pretrained language models (PLMs) can already capture implicit structures. Thus, a natural question arises: Is structural bias still a necessity in the context of PLMs? To answer the question, we propose to address the efficiency issues by using an adapter to integrate structural bias in the PLM and using a cheap-to-compute relative position structure in place of the syntactic dependency structure. Benchmarking evaluation is conducted on the SemEval datasets. The results show that our proposed structural adapter is beneficial to PLMs and achieves state-of-the-art performance over a range of strong baselines, yet with a light parameter demand and low latency. Meanwhile, we give rise to the concern that the current evaluation default with data of small scale is under-confident. Consequently, we release a large-scale dataset for ASTE. The results on the new dataset hint that the structural adapter is confidently effective and efficient to a large scale. Overall, we draw the conclusion that structural bias shall still be a necessity even with PLMs., 10 pages, 4 figures, 5 tables, accepted to COLING 2022, code is available at https://github.com/GeneZC/StructBias

Key to species of Neopheosia 1 Male genitalia lack of gnathos........................................................................... 2 - Male genitalia with a gnathos............................................................................ 4 2 Socii shorter than uncus................................................................... N. mandschurica - Socii longer than uncus................................................................................ 3 3 The costal valval process is long and narrow......................................................... N. atrifusa - The costal valval process is thicker and shorter....................................................... N. mariae 4 Forewing ground colour ash grey, medial fasciae light grey; valval shape resembles rectangular, tip has a small flap; sacculus well developed.............................................................................. N. albiplaga - Forewing ground colour orange-brown, apex with a blackish mark.............................................. 5 5 Vein M2, M3 of forewing are with more or less blackish mark; socii in male genitalia slender.................. N. fasciata - Vein M 2, M 3 of forewing with blackish mark constantly absent; a dark brown triangle on vein CuA 2 present; socius short and strongly sclerotized; valval tip has a small flap, costa carries a flap; signum rounded and small.... N. pseudofasciata sp. nov., Published as part of Zhang, Yu-Long, Wang, Min & Ma, Fang-Zhou, 2022, Neopheosia pseudofasciata sp. nov., a new species of Notodontidae (Lepidoptera) from Wuyishan National Park, southeastern China, pp. 589-594 in Zootaxa 5168 (5) on page 593, DOI: 10.11646/zootaxa.5168.5.7, http://zenodo.org/record/6911626

Neopheosia pseudofasciata sp. nov. Type material. Holotype: male, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 745 m, 2-V-2021, leg. Min Wang & Liping Zhou (SCAU Neo02). Paratypes: 1 male, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 750m, 21-V-2021, leg. Min Wang & Yulong Zhang (SCAU Neo 01); 1 female, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 700m, 1-V-2021, leg. Min Wang & Liping Zhou (SCAU Neo 03); 1 male, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 460m, 3-V-2021, leg. Min Wang & Liping Zhou (SCAU Neo 11); 1 male, CHINA, NE Jiangxi / Fujian Wuyishan, altitude 1600m, 20º56’N 117º25’E, IV-2002 (ASPC). Diagnosis. The new species is similar to N. fasciata, but the blackish mark constantly absent in M 3 and M 2 area in the forewing. A dark brown triangle spot on vein CuA 2 is present, and fills the space between M 2 and M 3. In male genitalia, gnathos robust (it is slender in fasciata), the socii strongly curved medially (they are bent slightly in fasciata), costa has a diagnostically important process (this process is absent in fasciata), two signa are present, the bigger one is shovel-shaped, and the small one is oval (oval signum is large in fasciata). Description. Male (Fig. 1). Head and frons covered with greyish hairs; antennae bipectinate at basal half, but are filiform over the apical half to the tip; palpi weakly sclerotized. Forewing length ca. 20 mm. Forewing elongated, ground colour yellow with blackish marks similar with N. fasciata; hindwing pale yellow, tornus black, termen with a line of black spots, abdomen with fuscous hair. Male genitalia (Fig. 11). Uncus bifurcated; gnathos robust; socius strongly curved medially, robust at its base, slender terminally; valva broad, tip flapped; costa carries a process basely which is diagnostically important; saccus broad. Female (Fig. 3). Forewing length 25 mm, characters basically are the same as for male, but the size of moth is usually larger, antennae filiform, abdomen short and thick, covered with greyish hair. Female genitalia (Fig. 13) with short apophyses posteriores and anteriores; ductus bursae with two signa, the bigger one is shovel-shaped, the second one is small and oval. Distribution. Endemic in SE China, Fujian, Wuyishan National Park. Etymology. The specific name pseudofasciata is derived from the other specific name fasciata (Moore) due to the resemblance of the present new species in external characters to the species N. fasciata. Remarks. Morphologically, the new species is very similar to N. fasciata, but it can be distinguished in forewing pattern and genitalia characters of both sexes. Molecularly (Fig. 15), the lineage of the new species has a strong support (bootstrap=100) and the genetic distance between the new species and N. fasciata reached from 6.9% to 7.4%; the genetic distance between some genera of Dicranurinae is more than 10%. Furthermore, the two species N. pseudofasciata and N. fasciata are distributed sympatrically. Hence, the validity of the new species is well supported by morphological, molecular and geographic evidence. For the convenience of identification, a key to Neopheosia species is given below, Published as part of Zhang, Yu-Long, Wang, Min & Ma, Fang-Zhou, 2022, Neopheosia pseudofasciata sp. nov., a new species of Notodontidae (Lepidoptera) from Wuyishan National Park, southeastern China, pp. 589-594 in Zootaxa 5168 (5) on pages 590-593, DOI: 10.11646/zootaxa.5168.5.7, http://zenodo.org/record/6911626, {"references":["Moore, E. (1888) Descriptions of new genera and species of Lepidoptera Heterocera, collected by Rev. JH Hocking, chiefly in the Kangra district, NW Himalaya. Proceedings of the Zoological Society of London, 56, 1, 390 - 416. https: // doi. org / 10.1111 / j. 1469 - 7998.1888. tb 06720. x"]}

Neopheosia pseudofasciata sp. nov. Type material. Holotype: male, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 745 m, 2-V-2021, leg. Min Wang & Liping Zhou (SCAU Neo02). Paratypes: 1 male, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 750m, 21-V-2021, leg. Min Wang & Yulong Zhang (SCAU Neo 01); 1 female, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 700m, 1-V-2021, leg. Min Wang & Liping Zhou (SCAU Neo 03); 1 male, CHINA, Fujian Province, Nanping City, Wuyishan National Park, altitude 460m, 3-V-2021, leg. Min Wang & Liping Zhou (SCAU Neo 11); 1 male, CHINA, NE Jiangxi / Fujian Wuyishan, altitude 1600m, 20º56’N 117º25’E, IV-2002 (ASPC). Diagnosis. The new species is similar to N. fasciata, but the blackish mark constantly absent in M 3 and M 2 area in the forewing. A dark brown triangle spot on vein CuA 2 is present, and fills the space between M 2 and M 3. In male genitalia, gnathos robust (it is slender in fasciata), the socii strongly curved medially (they are bent slightly in fasciata), costa has a diagnostically important process (this process is absent in fasciata), two signa are present, the bigger one is shovel-shaped, and the small one is oval (oval signum is large in fasciata). Description. Male (Fig. 1). Head and frons covered with greyish hairs; antennae bipectinate at basal half, but are filiform over the apical half to the tip; palpi weakly sclerotized. Forewing length ca. 20 mm. Forewing elongated, ground colour yellow with blackish marks similar with N. fasciata; hindwing pale yellow, tornus black, termen with a line of black spots, abdomen with fuscous hair. Male genitalia (Fig. 11). Uncus bifurcated; gnathos robust; socius strongly curved medially, robust at its base, slender terminally; valva broad, tip flapped; costa carries a process basely which is diagnostically important; saccus broad. Female (Fig. 3). Forewing length 25 mm, characters basically are the same as for male, but the size of moth is usually larger, antennae filiform, abdomen short and thick, covered with greyish hair. Female genitalia (Fig. 13) with short apophyses posteriores and anteriores; ductus bursae with two signa, the bigger one is shovel-shaped, the second one is small and oval. Distribution. Endemic in SE China, Fujian, Wuyishan National Park. Etymology. The specific name pseudofasciata is derived from the other specific name fasciata (Moore) due to the resemblance of the present new species in external characters to the species N. fasciata. Remarks. Morphologically, the new species is very similar to N. fasciata, but it can be distinguished in forewing pattern and genitalia characters of both sexes. Molecularly (Fig. 15), the lineage of the new species has a strong support (bootstrap=100) and the genetic distance between the new species and N. fasciata reached from 6.9% to 7.4%; the genetic distance between some genera of Dicranurinae is more than 10%. Furthermore, the two species N. pseudofasciata and N. fasciata are distributed sympatrically. Hence, the validity of the new species is well supported by morphological, molecular and geographic evidence. For the convenience of identification, a key to Neopheosia species is given below

Aspect-based sentiment analysis (ABSA) aims at predicting sentiment polarity (SC) or extracting opinion span (OE) expressed towards a given aspect. Previous work in ABSA mostly relies on rather complicated aspect-specific feature induction. Recently, pretrained language models (PLMs), e.g., BERT, have been used as context modeling layers to simplify the feature induction structures and achieve state-of-the-art performance. However, such PLM-based context modeling can be not that aspect-specific. Therefore, a key question is left under-explored: how the aspect-specific context can be better modeled through PLMs? To answer the question, we attempt to enhance aspect-specific context modeling with PLM in a non-intrusive manner. We propose three aspect-specific input transformations, namely aspect companion, aspect prompt, and aspect marker. Informed by these transformations, non-intrusive aspect-specific PLMs can be achieved to promote the PLM to pay more attention to the aspect-specific context in a sentence. Additionally, we craft an adversarial benchmark for ABSA (advABSA) to see how aspect-specific modeling can impact model robustness. Extensive experimental results on standard and adversarial benchmarks for SC and OE demonstrate the effectiveness and robustness of the proposed method, yielding new state-of-the-art performance on OE and competitive performance on SC., 12 pages, accepted to NLPCC 2022

Multimodal emotion recognition in conversations (mERC) is an active research topic in natural language processing (NLP), which aims to predict human's emotional states in communications of multiple modalities, e,g., natural language and facial gestures. Innumerable implicit prejudices and preconceptions fill human language and conversations, leading to the question of whether the current data-driven mERC approaches produce a biased error. For example, such approaches may offer higher emotional scores on the utterances by females than males. In addition, the existing debias models mainly focus on gender or race, where multibias mitigation is still an unexplored task in mERC. In this work, we take the first step to solve these issues by proposing a series of approaches to mitigate five typical kinds of bias in textual utterances (i.e., gender, age, race, religion and LGBTQ+) and visual representations (i.e, gender and age), followed by a Multibias-Mitigated and sentiment Knowledge Enriched bi-modal Transformer (MMKET). Comprehensive experimental results show the effectiveness of the proposed model and prove that the debias operation has a great impact on the classification performance for mERC. We hope our study will benefit the development of bias mitigation in mERC and related emotion studies., 10 pages, 5 figures, accepted to NLPCC 2022

This paper mainly investigates the reductions of an integrable coupling of the Levi hierarchy and an expanding model of the (2+1)-dimensional Davey-Stewartson hierarchy. It is shown that the integrable coupling system of the Levi hierarchy possesses a quasi-Hamiltonian structure under certain constraints. Based on the Lie algebras construct, The type abstraction hierarchy scheme is used to gener?ate the (2+1)-dimensional expanding integrable model of the Davey-Stewartson hierarchy.

Objective To investigate the correlation between the expression of Hsa_circ_0026352 and the clinical characteristics of breast cancer(BC) patients, to evaluate the value of Hsa_circ_0026352 as a diagnostic marker of breast cancer. Methods Human circRNA microarray was used to screen the different expression of circRNAs in BC tissues. qRT-PCR was used to verify the expression of Hsa_circ_0026352 in BC tissue and peripheral blood. CircRNA structure were performed by circPrimer1.2 software. T-test, ANOVA analysis, curve regression analysis and ROC curve analysis were performed to determine the diagnostic values of Hsa_circ_0026352. Results Hsa_circ_0026352 was significantly down-regulated in both breast cancer tissues and peripheral blood (P < 0.01). The AUC of Hsa_circ_0026352 were 0.881 in tissues and 0.826 in peripheral blood (both P < 0.01). The relative expression of Hsa_circ_0026352 in peripheral blood of BC cancer patients was negatively correlated with CA153 level (P < 0.05). The AUC of Hsa_circ_0026352 in peripheral blood of patients with ER positive, early stage breast cancer and breast neoplasm metastasis were 0.710, 0.771 and 0.722 (all P < 0.01), respectively. Conclusion Hsa_circ_0026352 could be a novel predictive biomarker for diagnosis of BC.

Sirtuins, as regulators of metabolism and energy, have been found to play an important role in health and disease. Sirt1, the most widely studied member of the sirtuin family, can ameliorate oxidative stress, immune inflammation, autophagy, and mitochondrial homeostasis by deacetylating regulatory histone and nonhistone proteins. Notably, sirt1 has gradually gained attention in kidney disease research. Therefore, an evaluation of the overall distribution of publications concerning sirt1 based on bibliometric analysis methods to understand the thematic evolution and emerging research trends is necessary to discover topics with potential implications for kidney disease research. We conducted a bibliometric analysis of publications derived from the Web of Science Core Collection and found that publications concerning sirt1 have grown dramatically over the past 2 decades, especially in the past 5 years. Among these, the proportion of publications regarding kidney diseases have increased annually. China and the United States are major contributors to the study of sirt1, and Japanese researchers have made important contributions to the study of sirt1 in kidney disease. Obesity, and Alzheimer’s disease are hotspots diseases for the study of sirt1, while diabetic nephropathy is regarded as a research hotspot in the study of sirt1 in kidney disease. NAD+, oxidative stress, and p53 are the focus of the sirt1 research field. Autophagy and NLRP3 inflammasome are emerging research trends have gradually attracted the interest of scholars in sirt1, as well as in kidney disease. Notably, we also identified several potential research topics that may link sirt1 and kidney disease, which require further study, including immune function, metabolic reprogramming, and fecal microbiota.

Objectives To explore the perceptions of patients undergoing percutaneous coronary intervention (PCI) regarding their pre‐operative health education. Methods A qualitative study using semi‐structured, in‐depth interviews was conducted in one cardiology unit in China from July 2019 to December 2019. Purposeful sampling of 17 patients undergoing PCI was interviewed about their perceptions of pre‐operative health education. Thematic analysis of the transcribed data was then used to identify the themes. Results Four themes emerged from the data:(a) triple roles of pre‐operative education with the categories of relief (reliving fear); burden (leading to stress); and meaningless (changing nothing); (b) family member involvement with the categories of shared responsibility and family members’ duty; (c) facilitators in the process of pre‐operative health education with the categories of emotional support, plain language and individualized pre‐operative education; (d) inhibitors in the process of pre‐operative health education with the categories of contradiction and threatening words. Conclusions Pre‐operative health education for patients undergoing PCI should be aligned with the individual patients’ information‐seeking styles and personal differences, emphasizing individualized patient education. Traditional Chinese philosophy should be considered in the practice of pre‐operative education for patients undergoing PCI, which emphasizes family member involvement; at the same time, patient empowerment and self‐care should also be stressed. In addition, emotional support and plain language from health professionals are important in pre‐operative health education for patients undergoing PCI; contradiction should be avoided, and threatening words should be used with caution and with consideration for cultural variations during pre‐operative education for patients undergoing PCI.

Additional file 1: Table S1. Table S1. 2,321 differentially methylated markers were selected in model construction.

Additional file 3: Figures S1-S11. Fig. S1 The input DNA amount health, benign, and tumor tissue samples in sequencing analysis. Fig. S2 cfDNA methylation analysis of HCC diagnosis. Fig. S3 GO and KEGG pathway analysis of benign and cancer signals. Fig. S4 Unsupervised hierarchical clustering of tissue-derived 2321 methylation markers selected for HCC diagnosis in the tissue samples and plasma samples. Fig. S5 The specificity and sensitivity of MH model distinguishing tumor from healthy individuals in the training and the validation cohort. Fig. S6 The specificity and sensitivity of BH model distinguishing tumor from healthy individuals in the training and the validation cohort. Fig. S7 The comparison of non-tissue tissue-specific markers and tissue-specific markers in BH model contribution. Fig. S8 The specificity and sensitivity of MB model distinguishing tumor from healthy individuals in the training and the validation cohort. Fig. S9 The ROC of the HCC screening model (malignant score) for HCC diagnosis in the training and the validation cohort. Fig. S10 The correlation of malignant score and benign score with cause of cirrhosis (HCV or non-HCV). Fig. S11 Clinical characteristics of misclassified cirrhotic samples.

Additional file 2: Table S2. Table S2. Performance of tissue-derived markers in tissue-samples.

Atherosclerosis is a serious age‐related disease, which has a tremendous impact on health care globally. Macrophage inflammation is crucial for the initiation and progression of atherosclerosis, and microRNAs (miRNAs) recently have emerged as potent modulators of inflammation, while the underlying mechanisms of its involvement in homocysteine (Hcy)‐mediated macrophage inflammation of atherosclerosis remain largely unknown. Here, we demonstrated that elevated Hcy inhibits the expression of miR‐195‐3p, which in turn enhances IL‐31 expression and thereby causes the secretion of macrophages pro‐inflammatory factors IL‐1β, IL‐6 and TNF‐α and accelerate atherosclerosis. Furthermore, we identified that Hcy can induce DNA hypermethylation and H3K9 deacetylation of miR‐195‐3p promoter due to the increased the binding of DNMT3a and HDAC11 at its promoter. More importantly, Sp1 interacts with DNMT3a suppressed the binding of HDAC11 at miR‐195‐3p promoter and promoted its transcription. In summary, our results revealed a novel mechanism that transcriptional and epigenetic regulation of miR‐195‐3p inhibits macrophage inflammation through targeting IL‐31, which provides a candidate diagnostic marker and novel therapeutic target in cardiovascular diseases induced by Hcy., Proposed regulation model of miR‐195‐3p expression in Hcy‐induced macrophage inflammation during the formation of atherosclerotic plaque. Downregulation of miR‐195‐3p accelerated Hcy‐induced pro‐inflammation factor expression in macrophage and formation of plaques in ApoE−/− mice by targeting IL‐31, DNMT3a and HDAC11 are responsible for the downregulation of miR‐195‐3p, and Sp1 interacts with DNMT3a was essential for HDAC11 to bind to miR‐195‐3p promoter.

Wenxiao Jia,*,1 Jiaqin Zhang,*,2 Fang Ma,1 Shengjie Hao,1 Xue Li,1 Ruiting Guo,1 Qianqian Gao,1 Yundong Sun,1 Jihui Jia,1 Wenjuan Li11Key Laboratory for Experimental Teratology of Chinese Ministry of Education, The Shandong Provincial Key Laboratory of Infection and Immunology, Department of Microbiology, School of Basic Medical Sciences, Shandong University, Jinan, People’s Republic of China; 2Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen, People’s Republic of ChinaCorrespondence: Wenjuan LiKey Laboratory for Experimental Teratology of Chinese Ministry of Education, The Shandong Provincial Key Laboratory of Infection and Immunology, Department of Microbiology, School of Basic Medical Sciences, Shandong University, No. 44, Wenhuaxi Road, Jinan, Shangdong Province, People’s Republic of ChinaTel +86 5 318 838 2579Fax +86 5 318 838 2502Email wenjli@sdu.edu.cn*These authors contributed equally to this workBackground: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC.Method: Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells.Results: We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro.Conclusion: These findings may provide a potential therapeutic target for H. pylori-associated GC.Keywords: long noncoding RNA, THAP9-AS1, gastric cancer, Helicobacter pylori &nbsp

Long polar fimbria (LPF) is one of the few fimbrial adhesins of enterohemorrhagic Escherichia coli (E. coli) O157:H7 associated with colonization on host intestine, and both two types of LPF (including LPF1 and LPF2) play essential roles during the bacterial infection process. Though the fimbriae had been well studied in intestinal pathogenic E. coli strains, new evidences from our research revealed that it might be the key virulence for bovine mastitis pathogenic E. coli (MPEC) as well. This article summarizes the current knowledge on the LPF in E. coli, focusing on its genetic characteristics, prevalence, expression regulation, and adherence mechanism in different pathotypes of E. coli strains.

Natural and edible materials have attracted increasing attention in food packaging, which could overcome the serious environmental issues caused by conventional non-biodegradable synthetic packaging. In this work, gelatin nanofibers incorporated with two kinds of essential oil (EO), peppermint essential oil (PO) and chamomile essential oil (CO), were fabricated by electrospinning for potential edible packaging application. Electron microscopy showed that smooth and uniform morphology of the gelatin/EOs was obtained, and the diameter of nanofibers was mostly enlarged with the increase of the EO content. The proton nuclear magnetic resonance spectrum confirmed the existence of PO and CO in nanofibers after electrospinning. The addition of EOs led to an enhancement of the water contact angle of nanofibers. The antioxidant activity was significantly improved for the nanofibers loaded with CO, while the antibacteria activity against Escherichia coli and Staphylococcus aureus was better for the fibers with PO addition. The combination of half PO and half CO in nanofibers compensated for their respective limitations and exhibited optimum bioactivities. Finally, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay with NIH-3T3 fibroblasts demonstrated the absence of cytotoxicity of the gelatin/EO nanofibers. Thus, our studies suggest that the developed gelatin/PO/CO nanofiber could be a promising candidate for edible packaging.

The development of food packaging possessing bioactivities which could extend the shelf life of food has gained increased interest in recent years. In this study, gelatin nanofibers with encapsulated angelica essential oil (AEO) were fabricated via electrospinning. The morphology of gelatin/AEO nanofibers was examined by scanning electron microscopy (SEM) and the addition of AEO resulted in the increase of fiber diameter. The proton nuclear magnetic resonance (1H-NMR) spectra were measured to confirm the presence of AEO in nanofibers. The hydrophobic property of gelatin nanofibers was also found to be improved with the addition of AEO. The nanofibers incorporated with AEO showed significant antioxidant activity and inhibitory effect against both Gram-negative and Gram-positive bacteria in a concentration dependent manner. Furthermore, the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay demonstrated that the developed gelatin/AEO nanofibers revealed no cytotoxicity effect. Thus, gelatin nanofibers incorporated with AEO can be used as potential food packaging.

The function of natural killer (NK) cells, defending against virus infection and tumour progression, is regulated by multiple activating and inhibiting receptors expressed on NK cells, among which sialic acid-bind immunoglobulin-like lectins (Siglecs) act as a vital inhibitory group. Previous studies have shown that Siglec7 and Siglec9 are expressed on NK cells, which negatively regulate the function of NK cells and modulate the immune response through the interaction of sialic acid-containing ligands. Siglec7 and Siglec9 are very similar in distribution, gene encoding, protein sequences, ligand affinity, and functions in regulating the immune system against virus and cancers, but differences still exist between them. In this review, we aim to discuss the similarities and differences between Siglec7 and Siglec9 and analyze their functions in virus infection and tumour progression in order to develop better anti-viral and anti-tumor immunotherapy in the future.

Aiming at the problems of high leakage rate and high damage rate in mechanized picking of Camellia oleifera fruit, a branch shaking type picking machine was designed. According to the working principle of picking, the structure design of key components was completed, and the orthogonal test of three factors and three levels was designed. The results showed that the best combination of operating parameters was: picking time 45s, vibration frequency 8Hz, amplitude 5cm of picking head. The field test of Camellia oleifera fruit picking machine was carried out, and the picking rate was 91.2%, and the damage rate of flower bud was 18.2%.

The technology and equipment of this study can improve the technical level of the equipment for peeling and cleaning of Camellia oleifera fruit. Based on the biological characteristics of Camellia oleifera fruit, the size distribution model of Camellia oleifera fruit was established, and the equipment structure and key parameters were determined based on the working principle and design method of the shelling and cleaning mechanism. Based on the analysis of the movement track of the shell stripping and cleaning executive parts, the test scheme is established, and it is optimized that when the crankshaft speed is 240-260r/min and the track speed is 0.4-0.6m/s, the treatment capacity can reach the level of 2000kg/h, and the purification rate is more than 99%. When the vibration frequency of the vibrating motor is set to 50Hz and the horizontal inclination of the separation belt is 50 ° ∼ 55 °, the cleaning effect of the equipment is the best.

Intramolecular hydrogen bonds(IMHBs) can lead to different physicochemical characteristics of nitrophenols(NPs) that determine their environmental behavior. In the present work, to reveal the relationship between IMHB and nitrophenol reduction, the effects of IMHB on the molecular geometries and properties of a series of nitrophenols were investigated with density functional theory(DFT) calculations. The results of the geometry optimization and atoms-in-molecules(AIM) analysis indicate relatively strong IMHBs in ortho-substituted nitrophenols, whose stability could be significantly improved. In comparing the E LUMO and adiabatic electron affinities(AEA) of the nitrophenol isomers, the presence of IMHBs benefited the reductive degradation of NPs, consistent with a previous study. To gain an insight into the effect mechanism of IMHBs on the reductive degradation behavior of these molecules, the condensed electrophilicity Fukui index(f –), natural charges and Wiberg bond orders of these nitrophenol isomers were calculated. The calculations indicate that the electrophilic reactivity activity of the O atom on the nitro group could be significantly improved due to the formation of IMHBs, which results in the enhanced reductive degradation of ortho-substituted NPs.

In this research, the tantalum was chosen to be an alloying element into the surface layer of M50 steel to explore the microstructure after surface alloying treatment by high current pulsed electron beam. Different thickness of Ta films were deposited onto the M50 steel by DC magnetron sputtering before irradiation and the morphology of surface and cross section after surface alloying treatment were observed. The microstructure of alloying layer was investigated by X-ray diffraction and transmission electron microscope. The results revealed that alloying layer could be divided into three layers. The top layer was composed of δ-Fe phase with dendritic and small hollow spherical structure due to the segregation of alloying elements especially Ta. Some large spherical and a few dendritic microstructures could be observed at the second alloying layer; while there was only lath martensite microstructures at bottom of alloying layer.

Long non-coding RNA tissue differentiation-inducing non-protein coding (TINCR) is associated with the carcinogenesis of several cancers. However, little is known about the function and mechanism of TINCR in lung adenocarcinoma (LUAD). Here, we aimed to analyze expression of TINCR and elucidate its mechanistic involvement in the progression of LUAD. The expression of TINCR was investigated according to Gene Expression Profiling Interactive Analysis at first and then detected in 29 LUAD tissues and paired adjacent normal tissues using qRT-PCR. Results indicated that TINCR was evidently downregulated in LUAD. The association between TINCR and clinicopathological parameters was analyzed by Pearson's chi-square test, suggesting TINCR was closely correlated with TNM stage and lymph mode metastasis. Subsequently, the function role of TINCR was examined by gain- and loss-of-function studies in LUAD (A549 and NCI-H292) cells. As analyzed by the scratch wound-healing and transwell assays, results revealed that TINCR suppressed the migration and invasion of A549 and NCI-H292 cells. However, TINCR exerted no effects on the cell proliferation as determined by CCK8 assay. Furthermore, we reported that loss of Sp1 could inhibit TINCR expression. Expressions of miR-107/miR-1286 were detected by qRT-PCR assay in A549 and NCI-H292 cells after TINCR knockdown or overexpression. In addition, the direct binding ability of the predicted miR-107 or miR-1286 binding site on TINCR was validated by luciferase activity assay. Results indicated TINCR could constrain the expression of miR-107/miR-1286, and was a target of them in LUAD cells. Bioinformatics analyses showed that BTRC and RAB14 was the potential target gene of miR-107 and miR-1286, respectively. These data revealed a possible regulatory mechanism in which upregulation of TINCR induced by Sp1 could constrain the migration and invasion through regulating miR-107 or miR-1286 in LUAD cells. Conjointly, our findings provide a valuable insight into the regulatory mechanism of TINCR in LUAD, supportive to its potential of therapeutic target for LUAD patients.

Recently, unmanned aerial vehicles (UAVs) have rapidly emerged as a new technology in the fields of plant protection and pest controlling. In order to optimize the comprehensive performance of plant protection drones in large-area operation, a new regional module route planning method based on improved cattle farming reciprocating was proposed. Firstly, according to the characteristics of work area, the concept and method of regional module division were given. Secondly, the route planning problem was transformed into the operation module division problem, and the sub-area module and route planning with the least operation time and energy consumption were obtained. The simulation results showed our proposed method for regional modularized route planning reduced the number of operations of plant protection drones in large-area operation areas effectively, and shortened the total flight distance of plant protection drones. Compared with the traditional cattle-farming reciprocating route planning, the work time of route planning and operating electricity consumption were shortened about 16.7% and 10.8% respectively, demonstrating our proposed method was feasible. Additionally, this study can provide a reference for multi-plant protection drone route planning.

Fei Ye1,*, Qiong Lan Tang2,*, Fang Ma1,*, Lei Cai,1 Ming Chen,1 Xiao Xia Ran,1 Xiao Yu Wang,1 Xue Feng Jiang11Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan University, Guangzhou 510630, People’s Republic of China; 2Department of Pathology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, People’s Republic of China*These authors contributed equally to this workBackground/aims: Circular RNAs (circRNAs), a class of newly discovered endogenous noncoding RNAs, have shown large capabilities in gene regulation. Patients with the grade 3 endometrial cancer (EC) have a generally poor prognosis, and the specific role of circRNAs in the grade 3 EC remains unclear. This study aims to investigate the roles of circRNAs in the grade 3 EC.Methods: In the current study, we screened the expression profiles of circRNAs taken from two women with the grade 3 EC and adjacent non-cancerous endometrial tissue using circRNAs sequencing. Bioinformatic analyses were applied to study these differentially expressed circRNAs. Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) of six dysregulated circRNAs was performed to validate the sequencing results. Bioinformatic analyses, including the negative correlation network analyses of circRNAs–microRNAs (miRNAs)–messenger RNAs (mRNAs) and the Cytoscape, were used to delineate the interaction of circRNAs/miRNAs of the entire network.Results: Data of circRNA sequencing showed a significant change in 75,928 unique circRNAs (P

County-level performance scores for the 11 assessment modules for the 28 laboratories by laboratory type (public health and clinical), Guangxi and Guizhou Provinces, China 2014. (DOC 89 kb)

Prefecture-level performance scores for the 11 assessment modules for the 28 laboratories by laboratory type (public health and clinical), Guangxi and Guizhou Provinces, China 2014. (DOC 90 kb)

Performance scores combined across three administrative levels (county, prefecture and province) for the 11 assessment modules for the 28 laboratories by laboratory type (public health and clinical), Guangxi and Guizhou Provinces, China, 2014. (DOC 95 kb)

Provincial-level performance scores for the 11 assessment modules for the 28 laboratories by laboratory type (public health and clinical), Guangxi and Guizhou Provinces, China, 2014. (DOC 93 kb)

The characteristics of tannery wastewater are complex composition, high suspended substance (SS) and heavy sludge load. Adding the functional bacteria agent into the Aerobic system and Sludge digestion tank can improve the biological system activity and reduce sludge discharge. The pilot-scale study results show that: The removal of CODcr and NH3-N was improved after biological synergy. The average removal rate of CODcr was 90.1% and the average removal rate of NH3-N was 97.4%, both of which were higher than the control group. The excess sludge reduction of the system could reach 75.16%, and the effect of sludge reduction was obvious in the experimental group. After biological synergy, the number of bacteria in aerobic pool increased significantly, and the microbial flora migrated in a better direction.

Magnetic carbon@ferroferric oxide nanocatalyst (C@FONC), a composite material comprising powdered activated carbon (PAC) and ferroferric oxide nanocatalyst (FONC), was prepared using chemical co-precipitation. In this study, we evaluated the performance and complete pathway of tetracycline (TC) degradation in a C@FONC/H2O2 system. Moreover, the toxicity of TC and its intermediates was estimated. TC degradation in the C@FONC/H2O2 system followed pseudo-first-order kinetics, and the TC removal efficiency reached 97% in 120 min (TC0 = 150 ppm). The electron spin resonance (ESR) analysis and free radical quenching experiments indicated the generation of •OH and HO2• during the reaction. The investigation of the reaction mechanisms revealed the complementary performances between C@FONC adsorption and heterogeneous Fenton-like reaction in the TC degradation process. The stability of C@FONC recycling was also measured by multiple cycles of reactions, and the TC removal rate still reached 85.8% after nine cycles. In addition, the superparamagnetism and negligible iron leaching of the C@FONC/H2O2 system represented significant improvements relative to PAC and the conventional homogeneous Fenton process, making it a promising composite for applications in advanced oxidation processes.

The statutory derivative actions were introduced over ten years ago into China under the Chinese Company Law 2005 and into the United Kingdom under the Companies Act 2006. This paper conducts a comparative and empirical analysis of the development and effectiveness of statutory derivative actions in China and the UK. It also evaluates the rules on derivative actions in the Provisions of the Supreme People’s Court on Several Issues concerning the Application of the Company Law of the People's Republic of China IV, which became effective on 1st September 2017.

In this study, the long-term effects of ultrafine tourmaline particles (UTPs) on the removal of nitrogen in wastewater, activated sludge viability and microbial population dynamics at low temperatures were investigated. Although there was no significant effect on the effluent concentrations of nitrogen after long-term exposure to 1 g/L UTPs at low temperatures, the oxidation rate of NH4+-N and the accumulation rate of NO2−-N increased and the formation rate of NO3−-N decreased during the aerobic phase of sequencing batch reactors. However, long-term exposure to 1 g/L UTPs did not significantly affect the microbial community richness and the community diversity of activated sludge at low temperatures. The mechanism of tourmaline was studied by assessing the dominant functional species involved in biological nitrogen removal from wastewater. It was found that 1 g/L UTPs increased the removal rate of nitrogen by reducing the relative abundance of nitrite oxidizing bacteria and increasing the relative abundance of ammonia oxidizing bacteria after long-term operation at low temperatures.

Our previous work discovered that the histone demethylase JMJD2B (KDM4B) plays oncogenic roles in gastric carcinogenesis, but the regulatory mechanism of JMJD2B in gastric cancer has not been well defined. It has been revealed that microRNAs function as gene regulators by binding to the 3’UTR of mRNAs to inhibit gene expression. In this study, we found that miR-491-5p suppressed cell proliferation, invasion and migration by directly targeting the JMJD2B 3’UTR in gastric cancer. Moreover, miR-491-5p was decreased in GC tissues compared with adjacent normal tissues, and JMJD2B had the inverse expression pattern. In contrast to healthy individuals, GC patients had lower miR-491-5p expression in serum (P

Interview Guide. The interview guides developed for the study. (DOCX 15Â kb)

Additional file 1. Figure S1. Time courses of atrazine degradation of strain ZXY-2 containing different initial atrazine concentrations. Figure S2. Atrazine-degrading gene amplification of Arthrobacter sp. ZXY-2. Table S1. Comparison of degradation rates with reported atrazine-degrading strains. Table S2. Sequence of the primer pairs used in PCR study. Table S3. The detail information of three identified atzB proteins in the whole genome nucleotide sequence.

The catalytic conversion of Alcell (R) lignin in iso-propanol/formic acid mixtures (1: 1 mass ratio) was explored in a batch set-up using Ru/C as the catalyst (673 K, 4 h, 28% mass lignin intake on solvent). Lignin oils were obtained in good yields (71% mass yields on lignin input) and shown to consist of a mixture of mainly aromatics (10.5% mass yields on lignin input), alkylphenolics (6% mass yields on lignin input), catechols (8.7% mass yields on lignin input), guaiacols (1.3% mass yields on lignin input), and alkanes (5.2% mass yields on lignin input), the remainder being soluble higher molecular weight compounds (GCxGC-FID and GPC). The results for the catalytic experiments using formic acid were compared with those of a non-catalysed experiment and a catalytic hydrotreatment with molecular hydrogen and Ru/C in the absence of a solvent. Distinct differences in product yields and compositions were observed, and highest lignin oil yields were obtained by catalytic solvolysis (71% mass yields on lignin input) versus 18% mass yields on lignin input for noncatalytic solvolysis and 63% mass yields on lignin input for catalytic hydrotreatment. The effect of reaction time on oil yields and product composition was established and a reaction network involving depolymerisation, and hydro(-deoxy)genation pathways is proposed to explain the product yields and composition. Besides iso-isopropanol, the use of ethanol and methanol in combination with formic acid was also explored for catalytic solvolysis. Best results were obtained in methanol (4 h, 673 K) leading to a lignin oil (68% mass yields on lignin input) containing 11% mass yields on lignin input of alkylphenolics and 19% mass yields on lignin input of aromatics. (C) 2015 Elsevier Ltd. All rights reserved.

In order to improve the conversion efficiency of the solar cell, the ZnO/Ni multilayer thin film used in back electrode was prepared through the electroless plating and sol–gel methods. The morphology, structure and resistivity of the electroless nickel coating were measured by scanning electron microscopy, X-ray diffraction, and four-point probe resistivity tester, respectively. It was found that the nickel coating presented crystalline structure with the lowest resistivity of 4.33×10−5 Ω cm. The effect of the ZnO sol concentration, annealing temperature and the number of the spin-coating layers on the crystal structure, film morphology and photoelectric performance were also studied. The light trapping structure was found on the surface of ZnO layer when the sol concentration, annealing temperature and the number of the spin-coating layers were 0.75 mol/L, 300 °C and 4, respectively. The average transmittance in the visible light range (390–780 nm) was about 1.06% by visible spectrophotometer. The ZnO/Ni multilayer film could be used in back electrode for solar cell and would improve the properties of the solar cell.