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Background Vascular inflammation has been recognized as one of the key factors in the pathogenesis of acute coronary syndromes (ACS). Peri-coronary adipose tissue (PCAT) attenuation by computed tomography angiography (CTA) has emerged as a marker specific for coronary artery inflammation. We examined the relationship between clinical presentation and coronary artery inflammation assessed by PCAT attenuation and coronary plaque characteristics. Methods Patients with ACS or stable angina pectoris (SAP) who underwent pre-intervention coronary CTA and optical coherence tomography (OCT) were enrolled. PCAT attenuation was measured around the culprit lesion and in the proximal 40 mm of all coronary arteries. PCAT attenuation and OCT findings were compared between patients with ACS versus SAP. Results Among 471 patients (ACS: 198, SAP: 273), PCAT attenuation was higher in ACS patients than in SAP patients both at the culprit plaque level (−67.5±9.6 Hounsfield unit [HU] vs. −71.5±11.0 HU, p Conclusions PCAT attenuation at culprit plaque, culprit vessel, and pan-coronary levels was higher in ACS patients than in SAP patients. Vascular inflammation appears to play a crucial role in the development of ACS. Funding Acknowledgement Type of funding sources: None.

Background An incidence of cardiovascular events increases with age in women. The relationship between cardiovascular risk factors, and the underlying pathology and the prevalence of lipid plaques has not been systematically studied in different age groups in women presented with acute coronary syndromes (ACS). Purpose We investigated the underlying pathology and the prevalence of lipid plaques in culprit lesions by optical coherence tomography (OCT) in women with different risk factors. Methods A total of 382 women who underwent pre-intervention OCT imaging were included. The underlying pathology and the prevalence of lipid plaques in the culprit lesion was compared between women with and without cardiovascular risk factors (i.e. hypertension, smoking, hyperlipidemia, diabetes mellitus, family history and chronic kidney disease) in three different age groups (70 yr). Results The relative prevalence of plaque erosion was higher in younger women ( Conclusion The prevalence of plaque erosion was higher in younger women ( Funding Acknowledgement Type of funding sources: Foundation.

In recent years, dry sludge powder has attracted attention for reducing environmental impact as a method of reusing ready mixed concrete waste. Manufacturing process of the dry sludge powder is different from the ordinary portland cement. In the manufacturing process, there is drying process, and it is performed at high temperature. A characteristic of dry sludge powder is that it contains hydration products. However, few studies have focused on the effects of high temperature treatment and the hydration products contained in dry sludge powder. Therefore, in this study, we focused on the influence of hydration products contained in dry sludge powder and the high temperature treatment on hydration reaction. Firstly, we analyzed hydration products contained in the dry sludge powder. Next, the progress of hydration was examined by measuring the rate of heat of hydration. In addition, a sample was prepared by simulating the dry sludge powder process and it was treated at different temperatures and times. By examining this sample, the relationship between hydration product and hydration reaction was investigated. As a result, it was found that, as the amount of calcium hydroxide in the dry sludge powder decrease, the strength development becomes easier. Furthermore, the hydration potential of the dry sludge powder, can be derived by subtracting the ignition loss value of the raw dry sludge powder state from the measured ignition loss value.

Aims Cryoballoon ablation (CBA) is effective for atrial fibrillation (AF), however acute silent cerebral infarction (SCI) is not uncommon after CBA. This study aimed to clarify the relationship between the morphology of LAA and SCI. Methods and results From 2015 to 2019, 207 consecutive patients (65.1±11.4 years, 26.6% of female, 31.4% of persistent AF) who underwent CBA for AF at our hospital were enrolled. All patients were evaluated with computed tomography for LAA before CBA and divided into two groups as chicken wing (CW) group and non-CW group. SCI was evaluated with magnetic resonance imaging at the following day after CBA. As a result, 18.4% of patients have occurred SCI after CBA. Although the patients' background didn't differ between the two groups, the Hounsfield scale (LAA/Aorta ratio) was lower (non-CW vs. CW = 0.68±0.12 vs. 0.73±0.11, p=0.004), and LAA orifice size tended to be larger (16.9±4.6 vs. 15.8±4.1, p=0.053) in non-CW group. SCI significantly occurred in non-CW group (29.5% vs. 11.6%, p=0.003), especially in cauliflower shape of 45.0%. In multivariate analysis, non-CW (p=0.002, Odds ratio 3.2, 95% of CI 1.5–7.0), skipped DOAC before CBA (p=0.04, Odds ratio 6.1, 95% of CI 1.1–115.4), and touch-up ablation in left atrium (p=0.02, Odds ratio 2.8, 95% CI 1.2–6.5) were independent predictors of SCI. Conclusions SCI was not rare after CBA for AF. Morphology of LAA was strongly associated with SCI. Funding Acknowledgement Type of funding source: None

Phenotypic screening is one of the most practical approaches to the identification of mediators of behaviour, since it is difficult to model brain function in vitro, at a cellular level. We used a zebrafish (Danio rerio) behavioural assay to discover novel, natural, neuroactive compounds.A zebrafish behavioural assay was performed for seven natural compounds, obtained from plants. The behavioural profiles were compared to those of known psychoactive drugs. We characterised a natural compound exhibiting a behaviour profile similar to that of suvorexant, using in silico, in vitro and microarray expression analysis.The behavioural analysis performed in this study classified central nervous system drugs according to their mechanism. Zebrafish treated with a natural compound, 8b-(4'-Hydroxytigloyloxy) costunolide (8b), showed behaviour profiles similar to those of zebrafish treated with suvorexant, a known orexin antagonist. This behavioural assay was validated using in silico and in vitro assays, which revealed that the new compound was a dual orexin receptor antagonist. In addition, transcriptome analysis suggested that 8b might regulate the nuclear factor-κB (NF-κB) related pathway.We conclude that zebrafish phenotypic screening, combined with in silico assays and gene expression profiling, is a useful strategy to discover and characterize novel therapeutic compounds, including natural products.

Background Because soluble (or secreted) amyloid precursor protein-β (sAPPβ) and -α (sAPPα) possibly reflect pathological features of Alzheimer’s disease (AD), they are potential biomarker candidates for dementia disorders, including AD and mild cognitive impairment (MCI) due to AD (MCI-AD). However, controversial results have been reported regarding their alterations in the cerebrospinal fluid (CSF) of AD and MCI-AD patients. In this study, we re-assessed the utility of sAPPα and sAPPβ in CSF as diagnostic biomarkers of dementia disorders. Methods We used a modified and sensitive detection method to analyze sAPPs levels in CSF in four groups of patients: AD (N = 33), MCI-AD (N = 17), non-AD dementia (N = 27), and disease controls (N = 19). Phosphorylated tau (p-tau), total tau, and Aβ42 were also analyzed using standard methods. Results A strong correlation was observed between sAPPα and sAPPβ, consistent with previous reports. Both sAPPα and sAPPβ were highly correlated with p-tau and total tau, suggesting that sAPPs possibly reflect neuropathological changes in the brain. Levels of sAPPα were significantly higher in MCI-AD cases compared with non-AD and disease control cases, and those of sAPPβ were also significantly higher in MCI-AD and AD cases relative to other cases. A logistic regression analysis indicated that sAPPα and sAPPβ have good discriminative power for the diagnosis of MCI-AD. Conclusions Our findings collectively suggest that both sAPPs are pathologically relevant and potentially useful biomarkers for early and accurate diagnosis of dementia disorders. We also suggest that careful measurement is important in assessing the diagnostic utility of CSF sAPPs. Electronic supplementary material The online version of this article (10.1186/s40364-017-0108-5) contains supplementary material, which is available to authorized users.

The present study aimed to examine the effects of rice particles in test foods and their visual appearance on oral textural perceptions. One original and three filtered Amazake (a traditional Japanese beverage made from malted rice) preparations were used as test foods. Three physical measurements of the test foods were conducted at room temperature: linear spread test, viscosity, and concentration of rice particles. Results of these three measurements formed two groups with lesser and more filtered test foods. Sensory evaluation experiments using a paired comparison test in 32 healthy young participants revealed the following: 1) the estimates of “grittiness in the mouth” (mouth feel) and “grittiness at a glance” of the test foods also comprised two groups, similar to the results of the physical measurements, 2) estimates of textural “smoothness” resulted in two groups, with a half-range of two items of “grittiness”, and 3) estimates of “sweetness” and “odor intensity” were similar to each other than to the other three items. Functional relationships between physical properties and characteristics of the test foods and sensory evaluation of their grittiness (oral, textural, and visual) of Amazake are discussed.

Background Although Right Ventricular dysfunction (RVD) is one of the predictor of poor prognosis, it is believed that ischemic RVD after percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) restores quickly. Because right ventricular perfusion has more ischemic preconditioning compared with left ventricle, due to their complex perfusion system. However, little is known about the time courses of RVD after ACS-PCI andtheir prognosis. We evaluated the relationship between right ventricular branch slow flow phenomenon (RVB-SF) post ACS-PCI in right coronary artery (RCA) and RVD at 6–8 months follow-up. Method We retrospectively analyzed consecutive 82 patients who underwent PCI for ACS in proximal or mid portion of RCA from August 2011 to March 2018 in our institution. Finally, both baseline and follow-up data were obtained from 70 patients. We analyzed TIMI frame count (TFC) to confirm the presence of RVB-SF (TFC ≥40 frame) after PCI. We also analyzed right ventricular fractional area change (RVFAC) at baseline and follow-up using echocardiography to detect sustained RVD (RVFAC ≤35%). Result We divided the patients into two groups (RVB-SF: 36 patients, RVB non-SF: 34 patients). Patient clinical characteristics were similar in both groups (sex, age, risk factors, medication, onset to balloon time, left ventricular stroke volume, max creatine kinase). Baseline RVFAC and follow-up RVFAC was significantly smaller in RVB-SF than in RVB non-SF, respectively. (27.1±1.7% vs. 38.3±1.8%, 31.4±1.0% vs. 48.7±1.1%, P Conclusion RVB-SF findings after ACS-PCI for RCA could predict sustained RVD at mid-term follow-up, which may indicate future prolonged RVD.

Background Silent cerebral thromboembolism (CE) in catheter ablation for atrial fibrillation (AF) is not rare. Prior our study has reported lower incidence of silent CE in AF radiofrequency (RF) ablation using irrigated-tip catheter than conventional 4 or 8mm-tip catheter. In addition, the incidence of silent CE in AF ablation under direct oral anticoagulant (DOAC) was equivalent to continuous therapeutic warfarin. Recently pulmonary vein isolation (PVI) using cryoballoon (CB) has emerged as an alternative technique to RF ablation because some studies suggested that the efficacy for PVI by CB was equivalent to RF. However, incidence of silent CE in CB ablation under the use of DOAC is unknown. Objective We aimed to evaluate the incidence new silent CE in AF ablation using CB system compared with irrigated RF system under the use of DOAC. Methods 322 consecutive patients with paroxysmal or persistent AF (155 using CB system, 167 using RF system) who underwent the first AF ablation were taking DOAC more than one month prior to the procedure. Throughout AF ablation procedure, heparin was administered to maintain activated clotting time (ACT) between 300 and 400 seconds. Head MRI was performed in all patients within 24 hours after the procedure. Results In 14 (9.0%) patients using CB and in 20 (12.0%) patients using RF, head MRI showed new embolic lesions without neurological symptom (P=0.469). Although the amount of heparin during the procedure in group CB was significantly less than in group RF (16134±3125 U vs. 18689±5222 U; P Conclusions The incidence of silent CE in AF ablation using CB system was lower than RF system, although it was not a significant difference between different ablation techniques for AF. In AF ablation, the use of CB may be preferred rather than RF as ablation system in regard to risk reduction of thromboembolic complications.

Background Dual-antiplatelet therapy (DAPT) after second-generation drug eluting stent (2-DES) implantation reduced the risk of stent thrombosis and subsequent ischemic events, with an increase in bleeding risk. Although chronic kidney disease patients have high ischemic and bleeding risk, little is known about both risks in hemodialysis patients after 2-DES implantation during DAPT. Method From July 2009 to March 2017, we retrospectively analyzed post-discharge major adverse cardiac and cerebrovascular events [MACCE: cardiac death, myocardial infarction, target vessel revascularization (TVR) and cerebral infarction] and bleeding events in 644 consecutive patients during DAPT after 2-DES implantation. We divided them into 2 groups [102 hemodialysis (HD) and 518 non-hemodialysis (Non-HD) patients, mean age, 71±10 years] after excluding 24 patients (lost to follow up and peritoneal dialysis). Follow-up period was 49±24 months. Median DAPT duration was 12 months. The primary endpoint was MACCE. The secondary endpoint was bleeding events according to the Bleeding Academic Research Consortium (BARC) type 2, 3, or 5. MACCE and bleeding events were compared between HD and Non-HD by using the propensity score-matching (PSM) method. Results Among the 620 eligible patients, the primary and secondary events occurred in 207 (33.3%) and 76 (12.3%) patients, respectively. The rates of unadjusted MACCE [HD vs Non-HD: 53.9% vs 29.3%; Hazard ratio (HR) 2.39, p After 1-to-1 propensity score adjustment for baseline differences (hypertension, diabetes mellitus, low ejection fraction, low albumin, anemia, and high C-reactive protein), a total of 160 patients (80 HD vs 80 Non-HD) was created. The rate of MACCE [HD vs Non-HD: 52.5% vs 31.3%; adjusted HR 2.04, p Conclusions As a result of PSM, HD was shown to contribute to ischemic risk rather than bleeding risk. Even in the 2-DES era, HD was an independent risk factor of cardiac death and TVR. Therefore, further study on the current regimen of DAPT would be necessary while balancing both ischemic and bleeding risk.

Soluble amyloid-β (Aβ) oligomers (AβOs), which elicit neurotoxicity and synaptotoxicity, are thought to play an initiating role in the pathology of Alzheimer's disease (AD). Since AβOs are a key therapeutic target, we attempted to identify natural agents that reduce AβO neurotoxicity. Using an assay system in which primary cultured neurons are treated with AβOs, we found that Rhodiola rosea extracts and one of its main constituents, tyrosol, significantly inhibited AβO-induced caspase-3 activation. We then assessed the in vivo efficacy of tyrosol by oral administration of the compound into AD model (5XFAD) transgenic and non-transgenic mice from either 2 or 4 to 7 months of age. In both paradigms, tyrosol treatment did not affect body weights of mice. Immunohistochemical analysis revealed that the immunoreactivity of spinophilin, a dendritic synaptic protein, was significantly reduced in three hippocampal subregions of vehicle-treated AD mice compared with non-transgenic mice, which was reversed in tyrosol-treated AD mice. Tyrosol treatment also prevented the enhancement of 4-hydroxy-2-nonenal immunoreactivity in the hippocampal CA3 region of AD mice. By contrast, tyrosol administration did not affect Aβ accumulation, as evaluated by immunohistochemical and biochemical analyses. Moreover, the Barnes maze test showed that tyrosol administration modestly mitigated spatial memory impairment in AD mice. These findings collectively indicate that the natural agent tyrosol protects neurons against AβO neurotoxicity in vitro and ameliorates synaptic disturbance, oxidative stress responses, and cognitive impairment in vivo. We thus suggest that tyrosol is potentially an effective, safe, and unique drug candidate for AD.

Electronic states of a free-standing hydrogenated monolayer boron (HB) sheet were studied via soft X-ray spectroscopies at the B K-shell absorption edge and first-principles calculations. The HB sheet is semimetallic with electron and hole pockets at the Y and Γ points, respectively. The electron band results from the B-H-B bonds formed during synthesis from a MgB2 crystal, while the hole band is kept through the process and originates from a honeycomb lattice boron layer or borophene in MgB2. Our results suggest that the HB sheet is a promising two-dimensional material for realizing new boron-based or superconducting nanodevices.

The authors have designed and microfabricated millimetre-scale wing-shaped hybrid microstructures composed of rigid structures of single crystal silicon (SCS) substrates and flexible polyimide (PI) membranes. The wing-shaped microstructures mimicking insect flapping flight have been newly designed using a fluid-structure interaction analysis, and the hybrid microstructures based on the design method have been successfully microfabricated using a simple process flow with SCS substrates coated with PI membranes. Shape of the SCS parts in the hybrid microstructures have been successfully fabricated using deep reactive ion etching of the SCS substrate with small etching rate, and the wing plates of PI membranes have been prepared with the photolithography and the curing processes. The flexibility of the PI membranes of the fabricated hybrid microstructures was also confirmed using the bending test of the PI membranes.

We have designed and microfabricated millimeter-scale wing-shaped hybrid microstructures composed of rigid structures of single crystal silicon (SCS) and flexible polyimide (PI) membranes. The wing-shaped microstructures mimicking insect flapping flight have been designed using a fluid-structure interaction analysis, and have been microfabricated using microfabrication technologies with the SCS substrates coated with photosensitive polyimide membranes. The shape of the SCS parts of the hybrid microstructures have been fabricated using deep reactive ion etching (D-RIE) process. The flexible parts of PI membranes have been prepared using photolithography and the subsequent curing process of the micro-patterned photosensitive PI thin film. The wing-shaped hybrid microstructures fabricated have been observed, and the flexibility of the PI membrane part of the hybrid microstructures was also confirmed using the bending test of the PI membrane., 12th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2017), April 9-12, 2017, UCLA Meyer & Renee Luskin Conference Center, Los Angeles, California, USA

Summary Introduction It is well recognized that examinations of activated platelets (aPLTs) and platelet-activating capacity are very important to observe and prevent embolic diseases (events) such as ischemic stroke and myocardial infarction. Previously, we reported an appropriate measurement technique of aPLT for clinical assay. In this paper, we investigated stable conditions for measurement of activating capacity of platelets. Methods Blood samples were taken from healthy volunteers using anticoagulants of 2K-EDTA, sodium citrate and heparin, and platelets were stimulated with adenosine diphosphate (ADP) or collagen. We demonstrated platelet-activating capacity by detection of scattering light, absorbance, microscopic observation, and P-selectin (CD62P) expression. We also performed basic experiments in seven healthy volunteers to test the clinical application of these assays with monitoring aspirin therapy. Results We judged that samples of whole blood with 2K-EDTA were suitable for CD62P expression assay as functional assessments of platelet activity, because platelets treated with anticoagulants such as sodium citrate and heparin were extremely damaged after stimulation, and it was difficult to measure the CD62P expression by flow cytometry. For optimal results, samples should be tested within 1 h after the drawing of blood and stimulated with ADP or collagen for 10 min. The CD62P-positive platelet value of blood from volunteers who had taken aspirin was decreased, and platelet activation was inhibited as well. Conclusion The simultaneous assay of aPLT and platelet-activating capacity by CD62P detection using whole blood treated with the K2-EDTA anticoagulant was useful for the monitoring of antiplatelet drugs.

Background and study aim A prototype forward-viewing instrument has been developed for therapeutic endoscopic ultrasound (EUS)-guided fine needle aspiration (FNA). We had the opportunity to use this forward-viewing echo endoscope and to study its clinical usefulness, mainly for diagnostic EUS-FNA. Patients and methods The prototype forward-viewing echo endoscope was used for 15 months between November 2006 and March 2010, in a study group comprising 47 consecutive patients. Diagnostic EUS-FNA was done in 38 patients and the diagnostic accuracy of the forward-viewing device was compared with that from an oblique-viewing echo endoscope in reference patients who were matched by disease and puncture route. Therapeutic EUS was done in nine patients (pseudocyst drainage in six; celiac ganglia neurolysis, biliary drainage, and pancreatic duct drainage in one each). Results Diagnostic EUS-FNA provided a correct diagnosis in 97.4 % (37/38 patients), which was not significantly different from the 94.7 % (36/38) in the reference patients. Lesions considered difficult to access with an oblique-viewing scope, such as those located at the fornix, or the head of the pancreas, or associated with strictures, were easily punctured, as were those located at the body or tail of the pancreas or at the porta hepatis. Treatment was successful in all nine patients who underwent therapeutic EUS procedures. None of the 47 patients had any complications. Conclusions A forward-viewing echo endoscope that allows target sites to be punctured more perpendicularly with minimal effort, can be used for diagnostic EUS-FNA and this may be advantageous, depending on the site of target lesions.

For contributing to the ITER project and promoting a possible early realization of DEMO, since 2007 the International Fusion Energy Research Centre (IFERC) shall perform the activities on (1) DEMO Design and R&D Coordination, (2) Computational Simulation Centre, and (3) ITER Remote Experimentation Centre in the framework under the agreement between the Government of Japan and the European Atomic Energy Community for the joint implementation of the Broader Approach Activities in the field of fusion energy. The DEMO Design activity aims at establishing a common basis for DEMO Design, including design features of DEMO, possible common concepts of DEMO, a roadmap for DEMO, and so on. Based on the common interest of EU and Japan toward DEMO, the DEMO R&D activities have been planned and carried out for the areas which are related to DEMO blanket development: SiC/SiC composites, tritium technology, materials engineering, advanced neutron multiplier, and advanced tritium breeders for DEMO blanket. In the activity of the Computational Simulation Centre, the objective is to provide and exploit a supercomputer for large scale simulation activities to analyse experimental data on fusion plasmas, prepare scenarios for ITER operation, predict the performance of the ITER facilities and contribute to the DEMO Design.

Column-averaged volume mixing ratios of carbon dioxide (XCO2) during the period from January 2007 to May 2008 over Tsukuba, Japan, were derived using CO2 concentrations measured by Continuous CO2 Measuring Equipment (CME). The CMEs were installed on Japan Airlines Corporation (JAL) commercial airliners, which frequently fly to and from Narita Airport. It was assumed that CO2 profiles over Tsukuba and Narita are the same. CO2 profile data for 493 flights on clear-sky days were analyzed in order to calculate XCO2 with one of two ancillary datasets: "Tsukuba observational" data (rawinsonde and meteorological tower), or "global" forecast/reanalysis and climatological data (NCEP and CIRA-86). The amplitude of the seasonal variation of XCO2 using the ancillary data measured in Tsukuba (XCO2 (Tsukuba observational)) was determined by a least squares fit using a harmonic function to roughly evaluate the seasonal variation over Tsukuba. The highest and lowest values of the obtained fitted curve in 2007 for XCO2 (Tsukuba observational) were 386.4 ± 1.0 and 381.7 ± 1.0 ppm in May and September, respectively, where the errors represent 1 standard deviation of the fit residuals. The dependence of XCO2 on the type of ancillary dataset was evaluated. The average difference between XCO2 from global climatological data, XCO2 (global), and XCO2 (Tsukuba observational), i.e., the bias of XCO2 (global) based on XCO2 (Tsukuba observational), was found to be −0.621 ppm with a standard deviation of 0.682 ppm. The uncertainty of XCO2 (global) based on XCO2 (Tsukuba observational) was estimated to be 0.922 ppm. This small uncertainty relative to the GOSAT precision suggests that calculating XCO2 using data from airliners and global climatological data can be applied to the validation of GOSAT products for XCO2 over airports worldwide.

Column-averaged volume mixing ratios of carbon dioxide (XCO2) during the period from January 2007 to May 2008 over Tsukuba, Japan, were derived using CO2 concentrations measured by Continuous CO2 Measuring Equipment (CME). The CMEs were installed on Japan Airlines Corporation (JAL) commercial airliners, which frequently fly to and from Narita Airport. It was assumed that CO2 profiles over Tsukuba and Narita are the same. CO2 profile data for 493 flights on clear-sky days were analyzed in order to calculate XCO2 with one of two ancillary datasets: "Tsukuba observational" data (rawinsonde and meteorological tower), or "global" forecast/reanalysis and climatological data (NCEP and CIRA-86). The amplitude of the seasonal variation of XCO2 using the ancillary data measured in Tsukuba (XCO2 (Tsukuba observational)) was determined by a least squares fit using a harmonic function to roughly evaluate the seasonal variation over Tsukuba. The highest and lowest values of the obtained fitted curve in 2007 for XCO2 (Tsukuba observational) were 386.4 ± 1.0 and 381.7 ± 1.0 ppm in May and September, respectively, where the errors represent 1 standard deviation of the fit residuals. The dependence of XCO2 on the type of ancillary dataset was evaluated. The average difference between XCO2 from global climatological data, XCO2 (global), and XCO2 (Tsukuba observational), i.e., the bias of XCO2 (global) based on XCO2 (Tsukuba observational), was found to be −0.621 ppm with a standard deviation of 0.682 ppm. The uncertainty of XCO2 (global) based on XCO2 (Tsukuba observational) was estimated to be 0.922 ppm. This small uncertainty relative to the GOSAT precision suggests that calculating XCO2 using data from airliners and global climatological data can be applied to the validation of GOSAT products for XCO2 over airports worldwide.

We evaluated the effects of a substrate in the biosynthesis of nitric oxide (NO)-l-arginine (LARG)-on hepatic lesions caused by ischemia/reperfusion (I/R) injury in rabbit livers. Rabbits were pretreated with LARG (150 mg/kg IV) or saline solution 0.9% (SS) before the hepatic I/R procedure. The effects of LARG on hepatic injury were evaluated before and after I/R. The warm hepatic I/R procedure produced profound acute liver injury, as indicated by elevated values of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactic dehydrogenase (LDH), as well as a high apoptotic cell count. All changes were attenuated by treatment with LARG before the hepatic I/R procedure. These results suggested that LARG produced protective effects on hepatic I/R lesions. This protective effect of LARG was probably associated with blocking generation of superoxide anions during the hepatic I/R procedure.

We examined the effects of acute exercise on reaction time to visual stimuli presented in the central portion and periphery of the visual field. Twelve participants performed reaction time tasks at rest and during cycling at 65 % peak oxygen uptake in two visual conditions. We used circular black-and-white checkerboard patterns as visual stimuli. The participants were asked to respond as quickly as possible to pattern reversal of the visual stimulus by releasing a response button. Reaction time was fractionated into Premotor time and Motor time. Premotor time in the peripheral condition significantly increased (p < 0.025) during exercise (mean +/- SE; 195.9 +/- 7.9 ms) from that at rest (183.7 +/- 6.8 ms). Premotor time in the central condition did not differ between at rest (185.3 +/- 7.6 ms) and during exercise (188.4 +/- 6.6 ms). These data suggest that the ability to respond to visual stimuli presented in the periphery of the visual field is vulnerable to moderate to severe exercise, as compared with the ability to respond to visual stimuli presented in the central portion of the visual field. An exercise-induced increase in arousal level and a consequent narrowing of attentional focus would explain the present results.

Both negative and positive persistent photoconductivity (NPPC and PPPC) effects on the low-temperature transport properties were studied in the Al x Ga 1-x As y Sb 1-y /InAs quantum wells with a well width of L w = 15 and 50 nm. The irradiation of light with λ < 1.0 μm causes the NPPC, while the light with λ =1.6μm causes the PPPC. The Hall coefficient R H (B) in the dark or after illumination considerably increases with increasing B, indicating the coexistence of majority electrons and minority holes and allowing to analyze it by means of the two-carrier model. The PPPC effect leads to an increase in both density (n and p) and mobility of electrons and holes, while the NPPC inversely leads to their decrease, which was confirmed by the PPC effects on the SdH oscillations and the Hall resistance in the regime of quantum Hall effect in high magnetic fields. We have found the experimental relation, pμn 3 , so that the minority holes increases (decreases) much more rapidly than the majority electrons increases (decreases) in the PPPC (NPPC).

Low-temperature magneto-transport properties of Al x Ga 1− x As y Sb 1− y /InAs deep quantum wells (QWs) with different well widths ( L w =15–500 nm) and their dimensionality were studied. The Hall data were analyzed by means of the two-carrier model: coexistence of electrons and holes for L w =15–150 nm and existence of two types of electrons with different mobility for L w =300 and 500 nm. The profile of perpendicular magnetoresistance (MR), ρ xx ( B ⊥ ) for the two-dimensional electrons (2DEs) in narrow QWs ( L w =15 and 50 nm) can be divided into the following four regimes: (1) the negative MR due to the weak localization (WL) in extremely low B -fields, (2) the crossing of ρ xx for different temperatures at B c =1/ μ e in moderate B -fields due to the orbital effect on the electron–electron interaction, (3) the Shubnikov–de Haas oscillations and (4) the quantum Hall effect in high- B fields. As for the in-plane MR except in the very low-field region characterized by the WL, Δ ρ xx ( B ∥ )/ ρ 0 always starts from a negative one essentially independent of T , which was explained by the classical boundary scattering at the wall of the barrier in quasi-ballistic regime. The quantum Hall resistance of the electron–hole system has also been discussed.