1. Criopreserved ovarian tissue transplantation and restoration of bone metabolism in castrated rats
- Author
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Silva, Lígia Helena Ferreira Melo e, Silva, João Marcos de Meneses e, Salama, Mahmoud, Pinheiro, Luiz Gonzaga Porto, Lunardi, Franciele Osmarini, Silva, Paulo Goberlânio Barros da, Hirth, Carlos Gustavo, Lucena, Igor Freitas de, Gomes, Glauco Jorge da Costa, and Leite, José Alberto Dias
- Subjects
Cryopreservation ,Osteoporose Pós-Menopausa ,Estrogênios ,Tissue Transplantation ,Insuficiência Ovariana Primária ,Estrogens ,Menopause ,Primary Ovarian Insufficiency ,Osteoporosis, Postmenopausal ,Menopausa - Abstract
Objectives: to evaluate estradiol levels and autotransplantation heated ovarian tissue effects, after vitrification, on rats bone metabolism previously oophorectomized bilaterally. Methods: experimental study with 27 rats aged 11 to 12 weeks and weighing 200g to 300g, submitted to bilateral oophorectomy and ovarian tissue cryopreservation for subsequent reimplantation. Animals were divided into two groups, A and B, with 8 and 19 rats, respectively. Autotransplantation occurred in two periods according to castration time: after one week, in group A, and after one month in group B. Serum estradiol measurements and ovary and tibia histological analysis were performed before and after oophorectomy period (early or late) and one month after reimplantation. Results: in groups A and B, tibia median cortical thickness was 0.463±0.14mm (mean±SD) at the baseline, 0.360±0.14mm after oophorectomy and 0.445±0.17mm one month after reimplantation p
- Published
- 2018