133 results on '"Luděk Bláha"'
Search Results
2. Levels and risks of surface contamination by thirteen antineoplastic drugs in the Czech and Slovak hospitals and pharmacies
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Tereza Hojdarová, Lenka Doležalová, Lucie Bláhová, Šárka Kozáková, Jan Kuta, and Luděk Bláha
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Slovakia ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Antineoplastic Agents ,Pilot Projects ,Pharmacy ,Capecitabine ,Occupational Exposure ,Internal medicine ,medicine ,Humans ,Environmental Chemistry ,Ifosfamide ,Hospital pharmacy ,Cyclophosphamide ,Czech Republic ,Pharmacies ,Chemotherapy ,business.industry ,General Medicine ,Pollution ,Hospitals ,Oxaliplatin ,Irinotecan ,Docetaxel ,Equipment Contamination ,Fluorouracil ,business ,Environmental Monitoring ,medicine.drug - Abstract
The consumption of hazardous antineoplastic drugs (ADs) used in anticancer chemotherapies is steadily increasing representing thus risks to both human health and the environment. Hospitals may serve as a contamination source, and pharmacists preparing the antineoplastic drugs (ADs) as well as nurses administering chemotherapy and caring for oncology patients are among the healthcare professionals being highly exposed. Here, we present the results of systematic monitoring (2018-2020) of surface contamination by 13 ADs in the pharmacies and hospitals in the Czech Republic (CZ; large-scale monitoring, 20 workplaces) and Slovak Republic (SK; pilot study at 4 workplaces). The study evaluated contamination by three commonly monitored ADs, i.e., 5-fluorouracil (FU), cyclophosphamide (CP), and platinum (total Pt representing cis-, carbo-, and oxaliplatin) together with ten less explored ADs, i.e., gemcitabine (GEM), ifosfamide (IF), paclitaxel (PX), irinotecan (IRI), docetaxel (DOC), methotrexate (MET), etoposide (ETOP), capecitabine (CAP), imatinib (IMAT), and doxorubicin (DOX). Floors and desktop surfaces in hospitals (chemotherapy application rooms, nurse working areas) were found to be more contaminated, namely with CP and Pt, in both countries when compared to pharmacies. Comparison between the countries showed that hospital surfaces in SK are generally more contaminated (e.g., CP median was 20 times higher in SK), while some pharmacy areas in the CZ were more contamined in comparison with SK. The newly studied ADs were detected at lower concentrations in comparison to FU, CP, and Pt, but some markers (GEM, IF, PX, and IRI) were frequently observed, and adding these compounds to routine monitoring is recommended.
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- 2021
3. Degradation of Cylindrospermopsin using Advanced Non-Thermal Plasma Technologies
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Marcel Schneider, Raphael Rataj, Jürgen Kolb, and Luděk Bláha
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The application of non-thermal plasmas in wastewater and air purification received a lot of attention, but their potential application in drinking water treatment has scarcely been investigated. Classified as Advanced Oxidation Processes, plasmas ignited in water or at the air-water interface generate a vast range of reactive species capable of removing water contaminants. The efficiency to degrade cylindrospermopsin (CYN, cyanobacterial toxin) was compared for six different plasma sources. A spark discharge showed the most energy-efficient degradation, followed by the other investigated systems, which showed similar trends. Two approaches were selected for further in-depth study of the degradation efficiency and underlying mechanisms. For a follow-up detailed study, a corona-like and a dielectric barrier discharge were selected based on the CYN degradation efficiency, usability of the reactors and plasma-chemistry. For the corona-like plasma, the degradation efficiency increased with increasing voltage and solution pH. After 15 min of plasma treatment at pH ≥ 7.5, degradation of CYN even progressed without further plasma application. The pH-dependency was not observed for the dielectric barrier discharge (DBD), whose degradation efficiency increased with decreasing operating voltage. The corona-like plasma promotes degradation primarily via OH, whereas the DBD produces mainly O3 and NOx. The application of non-thermal plasmas (NTPs) appears to be an innovative and promising approach for effective removal of cyanotoxins such as cylindrospermopsin from drinking water.
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- 2022
4. Rapid extraction and analysis of oxidative stress and DNA damage biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) in urine: Application to a study with pregnant women
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Lucie Bláhová, Tomáš Janoš, Vicente Mustieles, Andrea Rodríguez-Carrillo, Mariana F. Fernández, and Luděk Bláha
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Oxidative stress ,Pregnancy ,8-OHdG ,Urine ,Placenta ,Public Health, Environmental and Occupational Health - Abstract
This study developed a sensitive extraction method for urine matrix (based on lyophilization, without the need for pre-cleaning by solid phase extraction), coupled to LC-MS/MS analysis of the biomarker 8-hydroxy-2′ - deoxyguanosine (8-OHdG). The methodology was validated in urine samples from a cohort of Spanish pregnant women collected during the first, second and third trimester of pregnancy, and urine samples collected within 24 h after delivery (n = 85). A detection and quantification limit of 0.01 and 0.05 μg/L, respectively, were established. The median 8-OHdG concentration was 2.18 μg/L (range 0.33–7.79); and the corresponding creatinineadjusted concentrations ranged from 1.04 to 13.12 with median of 4.48 μg 8-OHdG/g creatinine. The concentrations of non-adjusted 8-OHdG significantly decreased (p < 0.05) in the 3rd trimester and post-delivery urine samples when compared to the 1st trimester levels. 8-OHdG concentrations were further studied in placenta samples matching the same urine samples (n = 26), with a median value of 1.3 ng 8-OHdG/g of tissue. Placental 8-OHdG concentrations were correlated with urinary levels of non-adjusted 8-OHdG in the 3rd trimester. Considering the small cohort size, results must be interpreted with caution, however statistical analyses revealed elevated urinary non-adjusted 8-OHdG levels in the 1st trimester of mothers that delivered boys compared to those who delivered girls (p < 0.01). Increased urinary non-adjusted 8-OHdG concentrations at the time of delivery were significantly associated with clinical records (any type of clinical record during pregnancy; p < 0.05). The novel extraction and analytical method for the assessment of 8-OHdG is applicable for sensitive analysis of multiple analytes or biomarkers in urine matrix. This method could also be applied for other matrices such as blood or tissues. Our findings show that 8-OHdG in urine of pregnant women could predict oxidative stress in placenta and can be related to characteristics such as maternal obesity, mode of delivery and newborn sex., European Union through Horizon 2020 projects HBM4EU [grant number 733032], CETOCOEN Excellence [grant number 857560], Horizon Europe project PARC [grant agreement No 101057014], RECETOX Research Infrastructure (No LM2023069), MEYS, and the OP RDE (the CETOCOEN EXCELLENCE project No. CZ.02.1.01/0.0/0.0/ 17_043/0009632)
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- 2023
5. The efficiency of antineoplastic drug contamination removal by widely used disinfectants–laboratory and hospital studies
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Luděk Bláha, Jan Kuta, Šárka Kozáková, Lucie Bláhová, Lenka Doležalová, and Tereza Krovová
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Detergents ,chemistry.chemical_element ,Antineoplastic Agents ,Diamines ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Floors and Floorcoverings ,Peracetic acid ,Chlorine ,medicine ,Outpatient clinic ,Peracetic Acid ,030212 general & internal medicine ,Hydrogen peroxide ,Decontamination ,business.industry ,Public Health, Environmental and Occupational Health ,Hazardous drugs ,Hydrogen Peroxide ,Human decontamination ,Contamination ,Stainless Steel ,030210 environmental & occupational health ,Hospitals ,Pyrrolidinones ,Quaternary Ammonium Compounds ,chemistry ,Glutaral ,Equipment Contamination ,Glutaraldehyde ,Laboratories ,business ,Disinfectants ,medicine.drug - Abstract
Antineoplastic drugs (ADs) pose risks to healthcare staff. Surface disinfectants are used in hospitals to prevent microbial contamination but the efficiency of disinfectants to degrade ADs is not known. We studied nine disinfectants on ten ADs in the standardized laboratory and realistic in situ hospital conditions. A survey in 43 hospitals prioritized nine most commonly used disinfections based on different ingredients. These were tested on inert stainless steel and in situ on contaminated hospital flooring. The effects against ten ADs were studied by LC–MS/MS (Cyclophosphamide CP; Ifosfamide IF; Capecitabine CAP; Sunitinib SUN; Methotrexate MET; Doxorubicin DOX; Irinotecan IRI; Paclitaxel PX; 5-Fluorouracil FU) and ICP-MS (Pt as a marker of platinum-based ADs). Monitoring of the floor contamination in 26 hospitals showed that the most contaminated are the outpatient clinics that suffer from a large turnover of staff and patients and have limited preventive measures. The most frequent ADs were Pt, PX, FU and CP with maxima exceeding the recommended 1 ng/cm2 limit by up to 140 times. IRI, FU, MET, DOX and SUN were efficiently removed by hydrolysis in clean water and present thus lower occupational risk. Disinfectants based on hydrogen peroxide were efficient against PX and FU (> 70% degradation) but less against other ADs, such as carcinogenic CP or IF, IRI and CAP. The most efficient were the active chlorine and peracetic acid-based products, which however release irritating toxic vapors. The innovative in situ testing of ADs previously accumulated in hospital flooring showed highly problematic removal of carcinogenic CP and showed that alcohol-based disinfectants may mobilize persistent ADs contamination from deeper floor layers. Agents based on hydrogen peroxide, peracetic acid, quaternary ammonium salts, glutaraldehyde, glucoprotamine or detergents can be recommended for daily use for both disinfection and AD decontamination. However, they have variable efficiencies and should be supplemented by periodic use of strong chlorine-based disinfectants efficient also against the carcinogenic and persistent CP.
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- 2021
6. Experimental review of different plasma technologies for the degradation of cylindrospermopsin as model water pollutant
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Marcel Schneider, Raphael Rataj, Luděk Bláha, and Juergen F. Kolb
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General Chemical Engineering ,Environmental Chemistry ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2023
7. Levels And Risks of Antineoplastic Drugs In Households of Oncology Patients, Hospices And Retirement Homes
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Jan Kuta, Luděk Bláha, Šárka Kozáková, Lucie Bláhová, Lenka Doležalová, and Tereza Hojdarová
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business.industry ,Hazardous drugs ,Surface contamination ,Antineoplastic drugs ,Cyclophosphamide ,Patient homes ,Pharmacy ,030210 environmental & occupational health ,Pollution ,3. Good health ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Environmental health ,Health care ,Antineoplastic Drugs ,Medicine ,Elderly people ,Oncology patients ,business ,medicine.drug - Abstract
Background Contamination of the indoor environment by antineoplastic drugs (ADs) is known to pose health risks to the exposed staff in hospitals or pharmacies. ADs may also contaminate households of the patients receiving chemotherapy, but the exposure levels and potential risks to family members have not been studied. The objective was to provide an in-depth research of surface contamination by ADs inside homes focusing on the households of oncology patients, hospices, and retirement houses. Methods The study was carried out in 17 patient households, 2 hospices, and 3 retirement homes. Surfaces were sampled using a standardized approach and the wipe samples were analyzed by UPLC–MS for 11 organic ADs and by ICP-MS/MS for total Pt as a marker of Pt-based ADs. Results The main study included repeated samplings of surfaces (floors, desktops) in households of 17 ambulant oncology patients receiving different chemotherapies with cyclophosphamide (CP), platinum-based drugs (Pt), doxorubicin (DOX), 5-fluorouracil (FU) and others. Patients treated with chemotherapy were found to serve as a source of contamination for their households, representing thus a risk to sensitive family members such as children or elderly people. Carcinogenic CP was commonly found at relatively high concentrations, especially during the first 6 days after the chemotherapy (maximum 511 pg/cm2). Sweat seems to be a major medium for the spread of the contamination, and high and long-time persisting CP levels (traces still found after 6 months post-chemotherapy) were found on various desktops including kitchen dining tables. The pilot studies in hospices and retirement homes indicated rather lower exposure risks of the personnel but pointed to potential long-lasting contamination by Pt or some other persistent ADs such as ifosfamide (IF). Conclusions This is one of the first studies investigating the contamination by ADs in indoor environments outside of hospitals or pharmacies. Peak concentrations of the carcinogenic CP in households were comparable to those observed in hospitals, but the temporal exposures are likely to cause lower risks to family members and caregivers compared to the long-time occupationally exposed health care personnel. The information guidance flier with practical recommendations was prepared improving thus information as well as prevention of eventual risks for family members.
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- 2021
8. Stratification strength and light climate explain variation in chlorophyll a at the continental scale in a European multilake survey in a heatwave summer
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Christine Edwards, Yang Yang, Kinga Kwasizur, Agnieszka Napiórkowska-Krzebietke, Jeremy Fonvielle, David Parreño Duque, Mari Carmen Trapote, Triantafyllos Kaloudis, Magdalena Toporowska, Koray Ozhan, Marek Kruk, Pablo Urrutia-Cordero, Cayelan C. Carey, Joana Mankiewicz-Boczek, Judita Koreivienė, Andrea G. Bravo, Hana Nemova, Abdulkadir Yağcı, Eloísa Ramos-Rodríguez, Michał Niedźwiecki, Petar Žutinić, Carmen Cillero-Castro, Moritz Buck, Rodan Geriš, Maria G. Antoniou, Daphne Donis, Tunay Karan, Fuat Bilgin, Agnieszka Ochocka, Lea Tuvikene, Hans-Peter Grossart, Christos Avagianos, Boris Aleksovski, Roberto L. Palomino, Trine Perlt Warming, Kersti Kangro, Justyna Sieńska, Jessica Richardson, Meriç Albay, Rahmi Uysal, Elísabeth Fernández-Morán, João Morais, Valentini Maliaka, Daniel Szymański, Irma Vitonytė, Vítor Gonçalves, Burçin Önem, Jordi Noguero-Ribes, Mikołaj Kokociński, Biel Obrador, Edward Walusiak, Lisette N. de Senerpont Domis, Reyhan Akçaalan, Wojciech Pęczuła, Juan M. Soria, Kadir Çapkın, Meryem Beklioglu, J. A. Gálvez, Korhan Özkan, Petra M. Visser, Özden Fakioglu, Şakir Çinar, Leonardo Cerasino, Dominic Vachon, Victor C. Perello, Magdalena Grabowska, Laura Seelen, David García, Jolanda M. H. Verspagen, Svetislav Krstić, Sevasti-Kiriaki Zervou, Adriano Boscaini, Donald C. Pierson, Kerstin Häggqvist, Carmen Ferriol, Iwona Jasser, Anastasia Hiskia, Miquel Lürling, Elżbieta Wilk-Woźniak, Julita Dunalska, Núria Catalán, José María Blanco, Mehmet Ali Turan Koçer, Valerie McCarthy, Köker Latife, Jorge Juan Montes-Pérez, Itana Bokan Vucelić, Anđelka Plenković-Moraj, Tuğba Ongun Sevindik, Beata Madrecka-Witkowska, Kirsten Christoffersen, Pauliina Salmi, Estela Rodríguez-Pérez, Joanna Rosińska, Barbara Pawlik-Skowrońska, Ilona Gagala-Borowska, Wojciech Krztoń, Elvira Romans, Hans W. Paerl, Spela Remec-Rekar, Magdalena Frąk, Mete Yilmaz, Carmen Pérez-Martínez, Susana Romo, Nur Filiz, Luděk Bláha, Karl . Rothhaupt, Enrique Moreno-Ostos, Markéta Fránková, Iwona Kostrzewska-Szlakowska, Nikoletta Tsiarta, Mehmet Tahir Alp, Joan Gomà, Jūratė Karosienė, Agnieszka Pasztaleniec, Vitor Vasconcelos, Jūratė Kasperovičienė, Filip Stević, Hanna Mazur-Marzec, Maria J. van Herk, Alinne Gurjão de Oliveira, Agnieszka Bańkowska-Sobczak, Sigrid Haande, Ana Maria Antão-Geraldes, Birger Skjelbred, Jose Luis Cereijo, Anna Kozak, Uğur Işkın, Manthos Panou, Agnieszka Budzyńska, Faruk Maraşlıoğlu, Iveta Drastichova, Tanja Žuna Pfeiffer, Ksenija Savadova-Ratkus, R. Carballeira, Alo Laas, Giovanna Flaim, Lucia Chomova, Tina Elersek, Lauri Arvola, Dubravka Špoljarić Maronić, Justyna Kobos, Lars-Anders Hansson, Beata Messyasz, Daniel Frank Mcginnis, Valeriano Rodríguez, Monserrat Real, Spyros Gkelis, Nilsun Demir, Evanthia Mantzouki, Pablo Alcaraz-Párraga, Elżbieta Szeląg-Wasielewska, Micaela Vale, Carlos Rochera, Meral Apaydın Yağcı, Piotr Domek, Jordi Delgado-Martín, Tõnu Feldmann, Michał Wasilewicz, Hatice Tunca, Ülkü Nihan Tavşanoğlu, Marija Gligora Udovič, Ulrike Obertegger, Bárbara Úbeda, Elif Neyran Soylu, Aleksandra Pełechata, Kristiina Mustonen, Danielle Machado-Vieira, Hannah Cromie, Eti E. Levi, Maciej Karpowicz, Nusret Karakaya, Cafer Bulut, Kemal Celik, Lidia Nawrocka, Natalia Jakubowska-Krepska, Armand Hernández, Anna C. Santamans, Sven Teurlincx, Damian Chmura, Arda Özen, Pedro M. Raposeiro, Nico Salmaso, Bastiaan Willem Ibelings, Gizem Bezirci, Kristel Panksep, Antonio Camacho, Theodoros M. Triantis, Antonio Picazo, William Colom-Montero, Teresa Vegas-Vilarrúbia, Manel Leira, Ana García-Murcia, Mariusz Pełechaty, Yvon Verstijnen, Irene Gallego, Mehmet Cesur, Ryszard Gołdyn, Yılmaz, Mete, Ministerio de Ciencia, Innovación y Universidades (España), National Science Foundation (US), AKWA, Aquatic Ecology (AqE), Centre for Limnology (NIOO / CL), Freshwater and Marine Ecology (IBED, FNWI), IBED (FNWI), Fen Edebiyat Fakültesi, Lammi Biological Station, BAİBÜ, Mühendislik Fakültesi, Çevre Mühendisliği Bölümü, and Karakaya, Nusret
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0106 biological sciences ,Temperate ,Aquatic Ecology and Water Quality Management ,010504 meteorology & atmospheric sciences ,Chlorophyll a ,CYANOBACTERIAL BLOOMS ,Mediterranean ,Oceanography ,01 natural sciences ,Filamentous cyanobacteria ,PHYTOPLANKTON DYNAMICS ,Klimatforskning ,Photosystem-II ,Climate change ,Phytoplankton biomass ,chlorophyll ,Temperature anomaly ,Phytoplankton Dynamics ,media_common ,Filamentous Cyanobacteria ,Ecology ,plankton ,TEMPERATE ,Dissolved Organic-Matter ,Plan_S-Compliant_NO ,Art ,Eutrophication ,Biological Sciences ,6. Clean water ,Europe ,kesä ,international ,EUTROPHICATION ,1181 Ecology, evolutionary biology ,articles ,lämpötila ,GREEN-ALGAE ,Natural Sciences ,LAKES ,SHALLOW ,klorofylli ,Thermal stratificaiton ,Climate Research ,media_common.quotation_subject ,multilake survey ,Cyanobacterial Blooms ,Aquatic Science ,phytoplankton ,European lakes ,climate change ,large scale ,light ,stratification ,nutrients ,järvet ,Heat wave ,limnologia ,PHOTOSYSTEM-II ,Settore BIO/07 - ECOLOGIA ,ddc:570 ,Life Science ,biomassa (ekologia) ,0105 earth and related environmental sciences ,Ekologi ,Green-Algae ,WIMEK ,FILAMENTOUS CYANOBACTERIA ,010604 marine biology & hydrobiology ,ilmastonmuutokset ,mikrolevät ,Aquatische Ecologie en Waterkwaliteitsbeheer ,Surface temperature ,Lakes ,Shallow ,13. Climate action ,DISSOLVED ORGANIC-MATTER ,Phytoplankton ,kerrostuneisuus ,Humanities ,valo - Abstract
The authors acknowledge COST Action ES 1105 "CYANOCOST Cyanobacterial blooms and toxins in water resources: Occurrence impacts and management" and COST Action Global Change Biology ES 1201 NETLAKE -Networking Lake Observatories in Europe" for contributing to this study through networking and knowledge sharing with European experts in the field. We acknowledge the members of the Global Lake Ecological Observatory Network (GLEON) for their collaborative spirit and enthusiasm that inspired the grassroots effort of the EMLS. E.M. was supported by a grant from the Swiss State Secretariat for Education, Research and Innovation to Bas Ibelings and by supplementary funding from University of Geneva. We thank Wendy Beekman for the nutrient analysis. We thank Pieter Slot for assisting with the pigment analysis. We thank Dr. Ian Jones for valuable feedback on an earlier version of the manuscript. We thank the Leibniz Institute of Freshwater Ecology and the Aquatic Microbial Ecology Group for logistic and technical support of J. Fonvielle and H.-P. Grossart, and the Leibniz Association for financial support. H.P. was supported by the US National Science Foundation (1840715, 1831096). A.C.'s work was funded by the Spanish Agencia Estatal de Investigacion and EU funds through the project CLIMAWET (CGL2015-69557-R). The collection of data for Lough Erne and Lough Neagh were funded by the Department of Agriculture, Environment and Rural Affairs, Northern Ireland. We are grateful to Kristiina Vuorio from the Freshwater Centre of the Finnish Environment institute for her help in organizing, collecting and analysing samples by the University of Jyvaskyla and to Gerald Dorflinger from the Water Development Department of Cyprus for his assistance with the sampling in Cyprus and for granting the CUT team permission to use WDD's equipment. Finally, we would like to thank the numerous other assistants that helped realizing each local survey. Open access funding provided by Universite de Geneve., To determine the drivers of phytoplankton biomass, we collected standardized morphometric, physical, and biological data in 230 lakes across the Mediterranean, Continental, and Boreal climatic zones of the European continent. Multilinear regression models tested on this snapshot of mostly eutrophic lakes (median total phosphorus [TP] = 0.06 and total nitrogen [TN] = 0.7 mg L-1), and its subsets (2 depth types and 3 climatic zones), show that light climate and stratification strength were the most significant explanatory variables for chlorophyll a (Chl a) variance. TN was a significant predictor for phytoplankton biomass for shallow and continental lakes, while TP never appeared as an explanatory variable, suggesting that under high TP, light, which partially controls stratification strength, becomes limiting for phytoplankton development. Mediterranean lakes were the warmest yet most weakly stratified and had significantly less Chl a than Boreal lakes, where the temperature anomaly from the long-term average, during a summer heatwave was the highest (+4 degrees C) and showed a significant, exponential relationship with stratification strength. This European survey represents a summer snapshot of phytoplankton biomass and its drivers, and lends support that light and stratification metrics, which are both affected by climate change, are better predictors for phytoplankton biomass in nutrient-rich lakes than nutrient concentrations and surface temperature., European Cooperation in Science and Technology (COST) ES 1105 ES 1201, Swiss State Secretariat for Education, Research and Innovation, University of Geneva, Leibniz Association, National Science Foundation (NSF) 1840715 1831096, Spanish Agencia Estatal de Investigacion, European Commission CGL2015-69557-R, Department of Agriculture, Environment and Rural Affairs, Northern Ireland
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- 2021
9. Pesticide mixture toxicity assessment through in situ and laboratory approaches using embryo-larval stages of the pacific oyster (Magallana gigas)
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Lucie Bláhová, Alicia Romero-Ramirez, Patrice Gonzalez, Mathilde Barré, Bénédicte Morin, Corentin Gouffier, Jérôme Cachot, Eliška Kuchovská, Luděk Bláha, Masaryk University [Brno] (MUNI), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,Oyster ,Developmental toxicity ,Zoology ,Aquatic Science ,Oceanography ,010603 evolutionary biology ,01 natural sciences ,In situ ,Developmental abnormality ,biology.animal ,Animals ,Pacific oyster ,14. Life underwater ,Crassostrea ,Pesticides ,Larva ,biology ,010604 marine biology & hydrobiology ,fungi ,General Medicine ,Pesticide ,Arcachon Bay ,biology.organism_classification ,Pollution ,Sublethal effects ,Transplantation ,Mixtures ,Toxicity ,Coastal waters ,Gene expression ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Laboratories ,Bay ,Water Pollutants, Chemical ,Swimming behavior - Abstract
International audience; Worsened state of oysters in French Arcachon Bay, demand an investigation of possible causes. This study evaluated the effects of an environmentally relevant mixture of five common pesticides on the early-life stages of the Pacific oyster (Magallana gigas). Laboratory assays with artificial mixture and in situ transplantation were complementarily used to investigate a series of sublethal endpoints. The laboratory exposure revealed developmental toxicity at 0.32 μg/L, which corresponds to mixture concentrations in Arcachon Bay. Downregulation of some gene transcriptions was observed at environmental level. No difference in larvae development was revealed among the three sites in Arcachon Bay. This study was the first to evaluate locomotion of oyster larvae exposed in situ. Suspected poor water quality in the inner part of Arcachon Bay was reflected by impairment at the molecular level. In conclusion, current concentrations of the tested pesticides in Arcachon Bay hinder larval development and affect several biological functions.
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- 2021
10. Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas)
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Rocío López-Cabeza, Patrice Gonzalez, Mathilde Barré, Luděk Bláha, Lucie Bláhová, Jérôme Cachot, Bénédicte Morin, Corentin Gouffier, Eliška Kuchovská, Université de Bordeaux (UB), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)
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Oyster ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Gene Expression ,010501 environmental sciences ,01 natural sciences ,Andrology ,chemistry.chemical_compound ,Neonicotinoids ,Imidacloprid ,biology.animal ,Gene expression ,Environmental Chemistry ,Animals ,14. Life underwater ,Crassostrea ,Waste Management and Disposal ,ComputingMilieux_MISCELLANEOUS ,0105 earth and related environmental sciences ,EC50 ,Larva ,biology ,Pesticide ,Pacific oyster ,Triazoles ,biology.organism_classification ,Nitro Compounds ,Pollution ,Propiconazole ,chemistry ,13. Climate action ,[SDE]Environmental Sciences ,France ,Biomarkers ,Water Pollutants, Chemical - Abstract
Coastal areas are final recipients of various contaminants including pesticides. The effects of pesticides on non-target organisms are often unclear, especially at environmentally relevant concentrations. This study investigated the impacts of insecticide imidacloprid (IMI) and fungicide propiconazole (PRO), some of the most detected pesticides in the Arcachon Bay in France. This work also included the research of propiconazole nanoformulation (nanoPRO). The effects were assessed studying the development of the early life stages of the Pacific oyster (Magallana gigas). Oyster embryos were exposed for 24, 30, and 42 h (depending on the endpoint) at 24 degrees C to environmentally relevant concentrations of the two pesticides as well as to nanoPRO. The research focused on sublethal endpoints such as the presence of developmental malformations, alterations of locomotion patterns, or changes in the gene expression levels. No developmental abnormalities were observed after exposure to environmental concentrations detected in the Arcachon Bay in recent years (maximal detected concentration of IMI and PRO were 174 ng/L and 29 ng/L, respectively). EC50 of PRO and nanoPRO were comparable. 2.93 7..t 1.35 and 226 +/- 1.36 mg/L, while EC50 of IMI exceeded 200 mg/L IMI did not affect larval behavior. PRO affected larval movement trajectory and decreased average larvae swimming speed (2 mu g/L), while nanoPRO increased the maximal larvae swimming speed (0.02 mu g/L). PRO upregulated especially genes linked to reactive oxygen species (ROS) production and detoxification. NanoPRO effects on gene expression were less pronounced - half of the genes were altered in comparison with PRO. IMI induced a strong dose-response impact on the genes linked to the detoxification, ROS production, cell cycle, and apoptosis regulation. In conclusion, our results suggest that current pesticide concentrations detected in the Arcachon Bay are safe for the Pacific oyster early development. but they might have a small direct effect via altered gene expressions, whose longer-term impacts cannot be ruled out.
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- 2020
11. Correction to: Levels and risks of surface contamination by thirteen antineoplastic drugs in the Czech and Slovak hospitals and pharmacies
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Lenka Doležalová, Lucie Bláhová, Jan Kuta, Tereza Hojdarová, Šárka Kozáková, and Luděk Bláha
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Health, Toxicology and Mutagenesis ,Environmental Chemistry ,General Medicine ,Pollution - Published
- 2022
12. Treatment of cylindrospermopsin by hydroxyl and sulfate radicals: Does degradation equal detoxification?
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Pavel Babica, Marina F. Grossi, Zdenek Spacil, Darshak Gadara, Marcel Schneider, and Luděk Bláha
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advanced oxidation process ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Decarboxylation ,Health, Toxicology and Mutagenesis ,Radical ,Bacterial Toxins ,010501 environmental sciences ,01 natural sciences ,Hydroxylation ,chemistry.chemical_compound ,Alkaloids ,Detoxification ,Humans ,Environmental Chemistry ,Organic chemistry ,Uracil ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Cyanobacteria Toxins ,cyanotoxin ,Sulfates ,Advanced oxidation process ,toxicity ,hepatospheroid ,water treatment ,Pollution ,6. Clean water ,chemistry ,Toxicity ,Water treatment ,Cylindrospermopsin ,Oxidation-Reduction ,Water Pollutants, Chemical - Abstract
Drinking water treatment ultimately aims to provide safe and harmless drinking water. Therefore, the suitability of a treatment process should not only be assessed based on reducing the concentration os a pollutant concentration but, more importantly, on reducing its toxicity. Hence, the main objective of this study was to answer whether the degradation of a highly toxic compound of global concern for drinking water equals its detoxification. We, therefore, investigated the treatment of cylindrospermopsin (CYN) by center dot OH and SO4-center dot produced in Fenton and Fenton-like reactions. Although SO4-center dot radicals removed the toxin more effectively, both radical species substantially degraded CYN. The underlying degradation mechanisms were similar for both radical species and involved hydroxylation, dehydrogenation, decarboxylation, sulfate group removal, ring cleavage, and further fragmentation. The hydroxymethyl uracil and tricyclic guanidine moieties were the primary targets. Furthermore, the residual toxicity, assessed by a 3-dimensional human in vitro liver model, was substantially reduced during the treatment by both radical species. Although the results indicated that some of the formed degradation products might still be toxic, the overall reduction of the toxicity together with the proposed degradation pathways allowed us to conclude: "Yes, degradation of CYN equals its detoxification!".
- Published
- 2022
13. Innovative electrochemical biosensor for toxicological investigations on algae and cyanobacteria
- Author
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Anne Schweizer, Luděk Bláha, and Michael Pescheck
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Cyanobacteria ,biology ,Chemistry ,010401 analytical chemistry ,Biophysics ,Biosensing Techniques ,02 engineering and technology ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Photosynthesis ,01 natural sciences ,0104 chemical sciences ,Algal growth inhibition ,Algae ,Biochemistry ,Electrochemistry ,Electrochemical biosensor ,Physical and Theoretical Chemistry ,Bioprocess ,0210 nano-technology ,Biosensor ,Bacteria - Abstract
An electrochemical biosensor is presented that directly reflects the metabolic activity of prokaryotic and eukaryotic cells. This biosensor can be used measure the biological activity of bacteria, yeasts and mammalian cells. This makes the sensor interesting for various applications in industry and science. A possible application is bioprocess control, monitor activities from yeasts, bacteria and fungi to increase the yield. Other applications are starter culture quality studies in the food industry and cytoxicological evaluation with mammalian cells. Our latest investigations additionally indicate the applicability of the electrochemical biosensor to measure algae and cyanobacteria. In our investigations, we were able to show that it was also possible to detect photosynthetic organisms with the electrochemical measurement method, used for investigations on prokaryotic and eukaryotic organisms before. Therefore, this the present study demonstrates an alternative to using this electrochemical biosensor equipped with alga and cyanobacteria for toxicological investigations based on selected test chemicals. The results of this study show a good correlation with those from reference methods, such as the Algal Growth Inhibition Test and the Microtox Test. The advantages of the new electrochemical biosensor are easy handling and shorter measurement time by using different types of test organisms. The evaluation of the sensor signal is based on the current–time curves of a potentiostatic measurement produced by the detection of microbially reduced mediator molecules immobilized in a gel structure. The mediator molecules are reduced during the measurement process. The reduced mediator molecules produce a current signal, which rapidly provides information about the vigor and vitality of living bacteria, yeasts, fungi or cells.
- Published
- 2022
14. Advanced oxidation processes for the removal of cyanobacterial toxins from drinking water
- Author
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Luděk Bláha and Marcel Schneider
- Subjects
Pollutant ,Waste management ,Sulfate radical ,0207 environmental engineering ,02 engineering and technology ,010501 environmental sciences ,Cyanotoxin ,01 natural sciences ,Pollution ,Water production ,6. Clean water ,Fenton oxidation ,13. Climate action ,Environmental science ,Water treatment ,Water quality ,AOP ,Cylindrospermopsin ,Hydroxyl radical ,Microcystin ,Ozone ,UV ,020701 environmental engineering ,Surface water ,0105 earth and related environmental sciences - Abstract
Drinking water production faces many different challenges with one of them being naturally produced cyanobacterial toxins. Since pollutants become more abundant and persistent today, conventional water treatment is often no longer sufficient to provide adequate removal. Amongst other emerging technologies, advanced oxidation processes (AOPs) have a great potential to appropriately tackle this issue. This review addresses the economic and health risks posed by cyanotoxins and discusses their removal from drinking water by AOPs. The current state of knowledge on AOPs and their application for cyanotoxin degradation is synthesized to provide an overview on available techniques and effects of water quality, toxin- and technique-specific parameters on their degradation efficacy. The different AOPs are compared based on their efficiency and applicability, considering economic, practical and environmental aspects and their potential to generate toxic disinfection byproducts. For future research, more relevant studies to include the degradation of less explored cyanotoxins, toxin mixtures in actual surface water, assessment of residual toxicity and scale-up are recommended. Since actual surface water most likely contains more than just cyanotoxins, a multi-barrier approach consisting of a series of different physical, biological and chemical – especially oxidative – treatment steps is inevitable to ensure safe and high quality drinking water.
- Published
- 2020
15. Ecotoxicology of Environmental Pollutants
- Author
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Jakub Hofman and Luděk Bláha
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Pollutant ,Human toxicity ,Environmental remediation ,0207 environmental engineering ,Biota ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,13. Climate action ,Hazardous waste ,Environmental toxicology ,Environmental science ,Ecotoxicology ,Ecotoxicity ,020701 environmental engineering ,Environmental planning ,0105 earth and related environmental sciences - Abstract
The most discussed hazardous properties of chemicals with respect to environmental impacts include persistency, tendency to bioaccumulate in biota, human toxicity, and ecotoxicity to living biota. In this chapter, aimed primarily at students and non-professionals in ecotoxicology such as environmental engineers or remediation experts, brief introduction to ecotoxicology is provided. The chapter brings introductory insights into the concepts of ecotoxicology and effects of chemicals in biological systems including the discussion of concentration–response relationships. Further, we introduce methods and tools in the testing of ecotoxicity, i.e., bioassays, and discuss their applications in both prospective and retrospective hazard assessment. Finally, the chapter provides a simple overview of major contaminants and their documented ecotoxic impacts on living organisms in both aquatic and terrestrial ecosystems.
- Published
- 2020
16. The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice
- Author
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Klára Hilscherová, Kamil Křůmal, Marcela Buchtová, Bohumil Dočekal, Zuzana Nováková, Zbyněk Večeřa, Pavel Mikuška, Lucie Bláhová, Aleš Hampl, Lucie Vrlíková, Luděk Bláha, and Jana Dumková
- Subjects
Male ,DNA damage ,Biomedical Engineering ,010501 environmental sciences ,Pharmacology ,Toxicology ,medicine.disease_cause ,Kidney ,01 natural sciences ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,Membrane Lipids ,Mice ,TBARS ,medicine ,Animals ,Humans ,Lung ,030304 developmental biology ,0105 earth and related environmental sciences ,Inflammation ,0303 health sciences ,Inhalation Exposure ,Mice, Inbred ICR ,Inhalation ,Chemistry ,Toxicity Tests, Subchronic ,Brain ,Oxides ,Malondialdehyde ,3. Good health ,Oxidative Stress ,medicine.anatomical_structure ,Lead ,Liver ,Toxicity ,Nanoparticles ,Lipid Peroxidation ,Oxidation-Reduction ,Oxidative stress ,Biomarkers ,DNA Damage - Abstract
Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles (PbONP; 192.5 mu g/m(3); 1.93 x 10(6) particles/cm(3)) for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E-2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment.
- Published
- 2019
17. CN32 Antineoplastic drugs in households of oncology patients
- Author
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Jan Kuta, Šárka Kozáková, Luděk Bláha, Lucie Bláhová, L. Dolezalova, and T. Krovova
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Oncology ,0303 health sciences ,medicine.medical_specialty ,business.industry ,Hematology ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Antineoplastic Drugs ,Oncology patients ,business ,030304 developmental biology - Published
- 2021
18. Critical assessment of the research outcomes of European birth cohorts: linking environmental factors with non-communicable diseases
- Author
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Vít Kandrnal, Luděk Bláha, and Pavel Piler
- Subjects
Databases, Factual ,030209 endocrinology & metabolism ,Context (language use) ,White People ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Environmental health ,Humans ,Medicine ,Longitudinal Studies ,030212 general & internal medicine ,Child ,business.industry ,Infant, Newborn ,Public Health, Environmental and Occupational Health ,Environmental Exposure ,General Medicine ,Environmental exposure ,3. Good health ,Europe ,Open source ,Critical assessment ,Citation ,business ,Birth cohort ,Cohort study - Abstract
Objectives The objective of this review paper was to stimulate collaborative discussions toward the development of a general concept of an open source protocol for a feasible and efficient longitudinal birth cohort study exploring non-communicable diseases (NCDs), their multifactorial etiology and relations between various risk factors. Study design The present paper systematically reviews the design of existing birth cohorts in Europe containing environmental exposure data, and assesses a quantity and quality of their research outcomes as their potential to be an effective tool for studying non-communicable diseases and their risk factors. Methods European birth cohorts with more than 3000 participants have been included in the study. A total number of scientific papers published in the internationally recognized journals and their impact factors and citation records were evaluated for all cohorts as surrogates for their efficiency to contribute to NCDs understanding and thus their prevention. Results The birth cohorts contributing most significantly to the NCD understanding shared common features: (i) study size between 10,000 and 15,000 mother–child pairs; (ii) repeated assessment of children from prenatal into adulthood; and (iii) availability of biological samples. Smaller cohorts and cohorts with a specific focus generated a lower number of publications; however, these often received considerably a higher number of citations. Conclusions General cohort studies with 10,000–15,000 mother–child pairs allow a broader context interpretation, publish a higher number of articles, and often lead to the formation of infrastructures for ‘spin-off (nested) studies’.
- Published
- 2017
19. Drinking water contaminants from epoxy resin-coated pipes: A field study
- Author
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Marek Pernica, Zdenĕk Šimek, Jan Kuta, Jonáš Lašňák, Johanna Rajasärkkä, and Luděk Bláha
- Subjects
endocrine system ,Bisphenol A ,Environmental Engineering ,Spray-on-lining ,Bisphenol F ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,chemistry.chemical_compound ,Phenols ,Humans ,Drinking water ,Water pipe ,Epoxy resin ,Benzhydryl Compounds ,Cities ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Civil and Structural Engineering ,Water Science and Technology ,Water contaminants ,Waste management ,Epoxy Resins ,urogenital system ,Chemistry ,Ecological Modeling ,Water Pollution ,Epoxy ,021001 nanoscience & nanotechnology ,Pollution ,6. Clean water ,Ecological Modelling ,13. Climate action ,Water temperature ,visual_art ,Environmental chemistry ,visual_art.visual_art_medium ,Water quality ,Leaching (metallurgy) ,0210 nano-technology ,Water Pollutants, Chemical ,hormones, hormone substitutes, and hormone antagonists - Abstract
Rehabilitation of aged drinking water pipes is an extensive renovation and increasingly topical in many European cities. Spray-on-lining of drinking water pipes is an alternative cost-effective rehabilitation technology in which the insides of pipes are relined with organic polymer. A commonly used polymer is epoxy resin consisting of monomer bisphenol A (BPA). Leaching of BPA from epoxy lining to drinking water has been a concern among public and authorities. Currently epoxy lining is not recommended in some countries. BPA leaching has been demonstrated in laboratory studies but the behavior and ageing process of epoxy lining in situ is not well known. In this study 6 locations with different age epoxy linings of drinking water pipes done using two distinct technologies were studied. While bisphenol F, 4-n-nonylphenol, and 4-t-octylphenol were rarely found and in trace concentrations, BPA was detected in majority of samples. Pipes lined with the older technology (LSE) leached more BPA than those with more recent technology (DonPro): maxima in cold water were 0.25 μg/L and 10 ng/L, respectively. Incubation of water in pipes 8–10 h prior to sampling increased BPA concentration in cold water 1.1–43-fold. Hot water temperature caused even more BPA leaching - at maximum 23.5 μg/L. The influence of ageing of epoxy lining on BPA leaching on could be shown in case of LSE technology: locations with 8–9 years old lining leached 4–20-fold more BPA compared to a location with 2-year-old lining. Analysis of metals showed that epoxy lining can reduce especially iron concentration in water. No significant burden to water could be shown by the analyzed 72 volatile organic compounds, including epichlorhydrin, precursor used in epoxy resin. Estrogenicity was detected in water samples with the highest BPA loads. Comparable responses of two yeast bioreporters (estrogen receptor α and BPA-targeted) indicated that bisphenol-like compounds were the main cause of estrogenicity. Compared to the estimated average daily BPA exposure, additional BPA load via cold drinking water in the studied locations was low, maximum 8.7%. However, hot water should also be considered as exposure source due to higher BPA concentrations. Epoxy lined locations should be monitored in future in order to evaluate ageing process and control increasing leaching of potentially harmful chemicals.
- Published
- 2016
- Full Text
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20. Climate finance and green growth: reconsidering climate-related institutions, investments, and priorities in Nepal
- Author
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Michal Bittner, Luděk Bláha, Batu Krishna Uprety, and Tek Jung Mahat
- Subjects
Adaptive capacity ,0208 environmental biotechnology ,Vulnerability ,Climate change ,Socioeconomic development ,02 engineering and technology ,010501 environmental sciences ,Climate Finance ,01 natural sciences ,Pollution ,020801 environmental engineering ,Green economy ,Green growth ,Development economics ,media_common.cataloged_instance ,European union ,0105 earth and related environmental sciences ,media_common - Abstract
Nepal, a least-developed, mountainous, and land-locked country is consistently ranked as one of the most vulnerable countries to the climate change. Poor socioeconomic development, rough and highly unstable geography, inadequate institutional capacity to deal with research, development and policy and mostly underdeveloped infrastructures, all have contributed to increasing vulnerability of communities and ecosystems, and have limited their adaptive capacity. Over the past decade, Nepal has made significant progress, particularly in developing and implementing policies and frameworks and establishing institutional mechanisms with the support of donor countries, UN and multilateral agencies. As the global climate politics is getting more complicated, international financing patterns—both climate and development finance—are shifting their ways, forcing the countries like Nepal to diversify the funding base for climate change actions and integrate them within national development plans and strategies. Using the data and information currently available, we analyze the existing financing situations, discuss the future scenarios and suggest policy recommendations to develop a set of long-term adaptation and impact mitigation strategies in specific and environmental change at large. When short-term adaptation strategies funded from existing financial arrangements and other related bilateral and multilateral sources particularly European countries, seem to be encouraging, we stress the need of “public–private partnership-driven full-fledged green economy” focusing on renewable energy and transport, agriculture and forestry, water and water-induced disasters, as well as tourism and hospitality.
- Published
- 2019
21. Cylindrospermopsin induces cellular stress and activation of ERK1/2 and p38 MAPK pathways in adult human liver stem cells
- Author
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Jan Raška, Iva Sovadinová, Aneta Dydowiczová, Luděk Bláha, Pavel Babica, and Lucie Čtveráčková
- Subjects
Programmed cell death ,Environmental Engineering ,Microcystins ,DNA damage ,Cell Survival ,MAP Kinase Signaling System ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,Bacterial Toxins ,02 engineering and technology ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,p38 Mitogen-Activated Protein Kinases ,Cell Line ,chemistry.chemical_compound ,Alkaloids ,Toxicity Tests ,medicine ,Environmental Chemistry ,Humans ,Uracil ,0105 earth and related environmental sciences ,Cell Proliferation ,Mitogen-Activated Protein Kinase 1 ,Mitogen-Activated Protein Kinase 3 ,Cyanobacteria Toxins ,Cell growth ,Stem Cells ,Public Health, Environmental and Occupational Health ,Hepatotoxin ,General Medicine ,General Chemistry ,Pollution ,020801 environmental engineering ,Cell biology ,Oxidative Stress ,chemistry ,Liver ,Cell culture ,Hepatocytes ,Marine Toxins ,Cylindrospermopsin ,Stem cell ,Reactive Oxygen Species ,Oxidative stress ,DNA Damage - Abstract
Cyanobacterial toxin cylindrospermopsin (CYN) is an emerging freshwater contaminant, whose expanding environmental occurrence might result into increased human health risks. CYN is potent hepatotoxin, with cytotoxicity and genotoxicity documented in primary hepatocytes or hepatoma cell lines. However, there is only limited information about CYN effects on adult human liver stem cells (LSCs), which play an important role in liver tissue development, regeneration and repair. In our study with human liver cell line HL1-hT1 which expresses characteristics of LSCs, CYN was found to be cytotoxic and increasing cell death after 24–48 h exposure to concentrations >1 μM. Subcytotoxic 1 μM concentration did not induce cell death or membrane damage, but inhibited cellular processes related to energy production, leading to a growth stagnation after >72 h. Interestingly, these effects were not associated with increased DNA damage, reactive oxygen species production, or endoplasmic reticulum stress. However, CYN induced a sustained (24–48 h) activation of mitogen-activated protein kinases ERK1/2 and p38, and increased expression of stress-related transcription factor ATF3. Thus, LSCs were not primarily affected by CYN-induced genotoxicity and oxidative stress, but via activation of signaling and transcriptional pathways critical for regulation of cell proliferation, stress responses, cell survival and inflammation. Alterations of LSCs during CYN-induced liver injury, including the role of nongenotoxic mechanisms, should be therefore considered in mechanistic assessments of chronic CYN hepatotoxicity and hepatocarcinogenicity.
- Published
- 2018
22. Synthetic Biomimetic Polymethacrylates: Promising Platform for the Design of Anti-Cyanobacterial and Anti-Algal Agents
- Author
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Marie Mlnaříková, Iva Sovadinová, Kenichi Kuroda, Přemysl Mikula, Luděk Bláha, Pavel Babica, Enrico T. Nadres, and Haruko Takahashi
- Subjects
Cyanobacteria ,Polymers and Plastics ,Microorganism ,Chlamydomonas reinhardtii ,02 engineering and technology ,Photosynthesis ,cyanobacteria ,Article ,antimicrobials ,lcsh:QD241-441 ,03 medical and health sciences ,lcsh:Organic chemistry ,Algae ,Microcystis aeruginosa ,biomimetic polymers ,polymethacrylates ,030304 developmental biology ,algae ,0303 health sciences ,biology ,Chemistry ,Desmodesmus ,water treatment ,General Chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,6. Clean water ,Biochemistry ,Green algae ,0210 nano-technology - Abstract
Extensive, uncontrolled growth of algae and cyanobacteria is an environmental, public health, economic, and technical issue in managing natural and engineered water systems. Synthetic biomimetic polymers have been almost exclusively considered antimicrobial alternatives to conventional antibiotics to treat human bacterial infections. Very little is known about their applicability in an aquatic environment. Here, we introduce synthetic biomimetic polymethacrylates (SBPs) as a cost-effective and chemically facile, flexible platform for designing a new type of agent suitable for controlling and mitigating photosynthetic microorganisms. Since SBPs are cationic and membranolytic in heterotrophic bacteria, we hypothesized they could also interact with negatively charged cyanobacterial or algal cell walls and membranes. We demonstrated that SBPs inhibited the growth of aquatic photosynthetic organisms of concern, i.e., cyanobacteria (Microcystis aeruginosa and Synechococcus elongatus) and green algae (Chlamydomonas reinhardtii and Desmodesmus quadricauda), with 50% effective growth-inhibiting concentrations ranging between 95 nM and 6.5 μM. Additionally, SBPs exhibited algicidal effects on C. reinhardtii and cyanocidal effects on picocyanobacterium S. elongatus and microcystin-producing cyanobacterium M. aeruginosa. SBP copolymers, particularly those with moderate hydrophobic content, induced more potent cyanostatic and cyanocidal effects than homopolymers. Thus, biomimetic polymers are a promising platform for the design of anti-cyanobacterial and anti-algal agents for water treatment.
- Published
- 2021
23. Antifouling performance of photocatalytic superhydrophobic coatings against Klebsormidium alga
- Author
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Barbora Pijáková, Dana Marešová, Eva Trávníčková, and Luděk Bláha
- Subjects
Materials science ,biology ,Fouling ,Process Chemistry and Technology ,02 engineering and technology ,010501 environmental sciences ,021001 nanoscience & nanotechnology ,biology.organism_classification ,01 natural sciences ,Pollution ,Surface energy ,Superhydrophobic coating ,Contact angle ,Biofouling ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,13. Climate action ,Titanium dioxide ,Photocatalysis ,Chemical Engineering (miscellaneous) ,0210 nano-technology ,Klebsormidium ,Waste Management and Disposal ,0105 earth and related environmental sciences - Abstract
Colonization of man-made surface materials by algae and other microorganisms, i.e. biofouling, causes various aesthetical and health problems as well as deterioration of appearance and surface functioning. The present study investigated eight complex surfaces combining various TiO2, alkoxy-siloxane and hydrophobization agents that were combined to prevent growth of phototrophic filamentous alga Klebsormidium sp. Regarding photoactivity, NOx abatement at the studied surfaces has been improved by adding anti-aggregation substances such as bentonite. Surface properties were characterized by confocal microscopy, scanning electron microscopy, SEM, with the energy dispersive X-ray spectroscopy, EDX, and the contact angle measurements. Biological experiments with Klebsormidium alga were performed in a constructed flow-through system. The results indicated that superhydrophobicity, i.e. very low surface energy, was critical for efficient antifouling during the early colonization by algae. This was specifically demonstrated in two novel composite samples made of titanium dioxide and commercial hydrophobization agent UltraEverDry. Low surface energy of these materials (around 1.3 mJ/m2), high water contact angles (>140°) as well as decent photoactivity (NOx conversions by 16.7 % and 1.4 % in UV and VIS, respectively), provide a promising basis for further longer-term anti-fouling experiments. The present study introduces novel composite materials preventing the algal fouling, and it brings new evidences on the interactive effects on hydrophobic and photoactive surfaces.
- Published
- 2020
24. Metallothionein modulation in relation to cadmium bioaccumulation and age-dependent sensitivity of Chironomus riparius larvae
- Author
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Luděk Bláha, Vojtěch Adam, Jan Kuta, Klára Hilscherová, David Hynek, René Kizek, and Zuzana Toušová
- Subjects
Health, Toxicology and Mutagenesis ,ved/biology.organism_classification_rank.species ,chemistry.chemical_element ,Zoology ,010501 environmental sciences ,Biology ,01 natural sciences ,Chironomidae ,Toxicology ,chemistry.chemical_compound ,Toxicity Tests ,Animals ,Environmental Chemistry ,Ecotoxicology ,Metallothionein ,0105 earth and related environmental sciences ,Chironomus riparius ,Cadmium ,ved/biology ,fungi ,010401 analytical chemistry ,Environmental Exposure ,General Medicine ,biology.organism_classification ,Pollution ,0104 chemical sciences ,chemistry ,Larva ,Bioaccumulation ,Instar ,Chironomus ,Toxicant - Abstract
The goal of this study was to contribute to understanding of the mechanisms behind sensitivity differences between early and late instar larvae of Chironomus riparius and to address the influence of the differences in standard testing approaches on the toxicity evaluation. A 10-day contact sediment toxicity test was carried out to assess sensitivity to cadmium exposure in relation to different age and laboratory culture line origin of test organisms. Chironomid larvae of early (OECD 218 method) and late instar (US-EPA600/R-99/064 method) differed substantially in sensitivity of traditional endpoints (OECD: LOEC 50 and 10 μg Cd/g dry weight (dw); US-EPA: LOEC > 1000 and 100 μg Cd/g dw for survival and growth, respectively). Bioaccumulated cadmium and metallothioneins (MTs) concentrations were analyzed to investigate the role of MTs in reduced sensitivity to cadmium in late instar larvae. Metallothioneins were induced after treatment to greater Cd concentrations, but their levels in relation to cadmium body burdens did not fully explain low sensitivity of late instars to cadmium, which indicates some other effective way of detoxification in late instars. This study brings new information related to the role of MTs in age-dependent toxicant sensitivity and discusses the implications of divergence in data generated by chironomid sediment toxicity tests by standardized methods using different instars.
- Published
- 2016
25. CN22 Occupational risks of health care personnel administering antineoplastic drugs. Results of the large-scale monitoring in Czech and Slovak hospitals
- Author
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L. Dolezalova, Šárka Kozáková, Lucie Bláhová, Luděk Bláha, and Jan Kuta
- Subjects
Czech ,0303 health sciences ,Scale (ratio) ,business.industry ,Hematology ,language.human_language ,03 medical and health sciences ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Environmental health ,Health care ,language ,Antineoplastic Drugs ,Medicine ,Slovak ,business ,030304 developmental biology - Published
- 2020
26. Environmentally relevant mixture of S-metolachlor and its two metabolites affects thyroid metabolism in zebrafish embryos
- Author
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Bénédicte Morin, Patrice Gonzalez, Eliska Rozmankova, Marek Pipal, Luděk Bláha, Naveen Njattuvetty Chandran, Lucie Bláhová, Université de Bordeaux (UB), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)
- Subjects
Embryo, Nonmammalian ,animal structures ,Health, Toxicology and Mutagenesis ,Metabolite ,Thyroid Gland ,Retinoic acid ,Embryonic Development ,010501 environmental sciences ,Aquatic Science ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,CYP26A1 ,Acetamides ,medicine ,Animals ,Yolk sac ,Zebrafish ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,biology ,Herbicides ,Cytochrome P450 ,Drug Synergism ,Metabolism ,biology.organism_classification ,Molecular biology ,medicine.anatomical_structure ,chemistry ,13. Climate action ,Larva ,[SDE]Environmental Sciences ,biology.protein ,Ethanesulfonic acid ,Water Pollutants, Chemical - Abstract
Herbicides and their metabolites are often detected in water bodies where they may cause adverse effects to non-target organisms. Their effects at environmentally relevant concentrations are often unclear, especially concerning mixtures of pesticides. This study thus investigated the impacts of one of the most used herbicides: S-metolachlor and its two metabolites, metolachlor oxanilic acid (MOA) and metolachlor ethanesulfonic acid (MESA) on the development of zebrafish embryos (Danio rerio). Embryos were exposed to the individual substances and their environmentally relevant mixture until 120 hpf (hours post-fertilization). The focus was set on sublethal endpoints such as malformations, hatching success, length of fish larvae, spontaneous movements, heart rate and locomotion. Moreover, expression levels of eight genes linked to the thyroid system disruption, oxidative stress defense, mitochondrial metabolism, regulation of cell cycle and retinoic acid (RA) signaling pathway were analyzed. Exposure to S-metolachlor (1 mu g/L) and the pesticide mixture (1 mu g/L of each substance) significantly reduced spontaneous tail movements of 21 hpf embryos. Few rare developmental malformations were observed, but only in larvae exposed to more than 100 mu g/L of individual substances (craniofacial deformation, non-inflated gas bladder, yolk sac malabsorption) and to 30 mu g/L of each substance in the pesticide mixture (spine deformation). No effect on hatching success, length of larvae, heart rate or larvae locomotion were found. Strong responses were detected at the molecular level including induction of p53 gene regulating the cell cycle (the pesticide mixture - 1 mu g/L of each substance; MESA 30 mu g/L; and MOA 100 mu g/L), as induction of cyp26a1 gene encoding cytochrome P450 (pesticide mixture - 1 mu g/L of each substance). Genes implicated in the thyroid system regulation (dio2, thra, thrb) were all overexpressed by the environmentally relevant concentrations of the pesticide mixture (1 mu g/L of each substance) and MESA metabolite (1 mu g/L). Zebrafish thyroid system disruption was revealed by the overexpressed genes, as well as by some related developmental malformations (mainly gas bladder and yolk sac abnormalities), and reduced spontaneous tail movements. Thus, the thyroid system disruption represents a likely hypothesis behind the effects caused by the low environmental concentrations of S-metolachlor, its two metabolites and their mixture.
- Published
- 2020
27. Assessment of Hepatotoxic Potential of Cyanobacterial Toxins Using 3D In Vitro Model of Adult Human Liver Stem Cells
- Author
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James E. Trosko, Aneta Dydowiczová, Amrita Basu, Lucie Čtveráčková, Pavel Babica, Luděk Bláha, and Libor Jaša
- Subjects
0301 basic medicine ,Microcystins ,Bacterial Toxins ,Microcystin-LR ,010501 environmental sciences ,Cyanobacteria ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Environmental Chemistry ,Humans ,Cytotoxicity ,0105 earth and related environmental sciences ,Cyanobacteria Toxins ,Chemistry ,Cell growth ,Stem Cells ,Hepatotoxin ,General Chemistry ,Molecular biology ,030104 developmental biology ,Liver ,Hepatic stellate cell ,Marine Toxins ,Cylindrospermopsin ,Stem cell ,Marine toxin - Abstract
Cyanotoxins microcystin-LR (MC-LR) and cylindrospermopsin (CYN) represent hazardous waterborne contaminants and potent human hepatotoxins. However, in vitro monolayer cultures of hepatic cell lines were found to recapitulate, poorly, major hepatocyte-specific functions and inadequately predict hepatotoxic effects of MC-LR and CYN. We utilized 3-dimensional (3D), scaffold-free spheroid cultures of human telomerase-immortalized adult liver stem cells HL1-hT1 to evaluate hepatotoxic potential of MC-LR and CYN. In monolayer cultures of HL1-hT1 cells, MC-LR did not induce cytotoxic effects (EC50 > 10 micromol/L), while CYN inhibited cell growth and viability (48h-96h EC50 ≈ 5.5-0.6 micromol/L). Growth and viability of small growing spheroids were inhibited by both cyanotoxins (≥0.1 micromol/L) and were associated with blebbing and disintegration at the spheroid surface. Hepatospheroid damage and viability reduction were observed also in large mature spheroids, with viability 96h-EC50 values being 0.04 micromol/L for MC-LR and 0.1 micromol/L for CYN, and No Observed Effect Concentrations
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- 2018
28. Temperature effects explain continental scale distribution of cyanobacterial toxins
- Author
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Sigrid Haande, Christos Avagianos, Vítor Gonçalves, Lisette N. de Senerpont Domis, Carlos Rochera, Ana García-Murcia, Kerstin Häggqvist, Reyhan Akçaalan, Jordi Noguero-Ribes, Mariusz Pełechaty, Wojciech Krztoń, Hans-Peter Grossart, Jutta Fastner, Bárbara Úbeda, Wojciech Pęczuła, Nur Filiz, Justyna Kobos, Juan M. Soria, Elif Neyran Soylu, Lars-Anders Hansson, Filip Stević, Luděk Bláha, Hanna Mazur-Marzec, Jolanda M. H. Verspagen, Burçin Önem, Karl-Otto Rothhaupt, Nico Salmaso, Abdulkadir Yağcı, David Parreño Duque, Ksenija Savadova, Nusret Karakaya, Aleksandra Pełechata, Yvon Verstijnen, Carmen Pérez-Martínez, Pauliina Salmi, Gizem Bezirci, Tuğba Ongun Sevindik, Svetislav Krstić, Rahmi Uysal, Laura Seelen, Eloísa Ramos-Rodríguez, Spela Remec-Rekar, Sven Teurlincx, Monserrat Real, Meriç Albay, Donald C. Pierson, Susana Romo, Kristiina Mustonen, Kirsten Christoffersen, Valentini Maliaka, Estela Rodríguez-Pérez, Joanna Rosińska, Nilsun Demir, Mehmet Tahir Alp, Elvira Romans, João Morais, Daniel Szymański, Danielle Machado-Vieira, Damian Chmura, Evanthia Mantzouki, Teresa Vegas-Vilarrúbia, Antonio Picazo, Mikołaj Kokociński, Anastasia Hiskia, Christine Edwards, Yang Yang, Irma Vitonytė, Mehmet Cesur, Agnieszka Bańkowska-Sobczak, Iwona Kostrzewska-Szlakowska, Nikoletta Tsiarta, Anđelka Plenković-Moraj, Miquel Lürling, Ryszard Gołdyn, Kristel Panksep, Kemal Celik, Anna Kozak, Jose Luis Cereijo, Pablo Urrutia-Cordero, Petra M. Visser, Rodan Geriš, Uğur Işkın, Leonardo Cerasino, Kadir Çapkın, Victor C. Perello, Carmen Cillero-Castro, Arda Özen, Manel Leira, Enrique Moreno-Ostos, Şakir Çinar, Agnieszka Budzyńska, Faruk Maraşlıoğlu, Pedro M. Raposeiro, Theodoros M. Triantis, Agnieszka Pasztaleniec, Sevasti-Kiriaki Zervou, Elżbieta Wilk-Woźniak, Edward Walusiak, Kersti Kangro, Jorge Juan Montes-Pérez, Triantafyllos Kaloudis, Mari Carmen Trapote, Pablo Alcaraz-Párraga, José María Blanco, Marek Kruk, Hans W. Paerl, Lidia Nawrocka, Meryem Beklioglu, Antonio Camacho, Moritz Buck, Biel Obrador, Ilona Gagala, Lauri Arvola, Elżbieta Szeląg-Wasielewska, Petar Žutinić, Giovanna Flaim, Núria Catalán, R. Carballeira, Alinne Gurjão de Oliveira, Magdalena Frąk, Alo Laas, Magdalena Grabowska, Dubravka Špoljarić Maronić, Meral Apaydın Yağcı, Itana Bokan Vucelić, Ana Maria Antão-Geraldes, Tõnu Feldmann, Natalia Jakubowska-Krepska, Trine Perlt Warming, Armand Hernández, Anna C. Santamans, Fuat Bilgin, Cayelan C. Carey, Joana Mankiewicz-Boczek, Elísabeth Fernández-Morán, Mete Yilmaz, Iwona Jasser, Boris Aleksovski, Michał Wasilewicz, Agnieszka Ochocka, David García, Lea Tuvikene, Roberto L. Palomino, B.W. Ibelings, Hatice Tunca, Birger Skjelbred, Joan Gomà, Jūratė Karosienė, Maria G. Antoniou, Vitor Vasconcelos, Mehmet Ali Turan Koçer, Eti E. Levi, Markéta Fránková, Beata Madrecka, Barbara Pawlik-Skowrońska, Jeremy Fonvielle, Korhan Özkan, Maciej Karpowicz, Özden Fakioglu, Lucia Chomova, Magdalena Toporowska, Ülkü Nihan Tavşanoğlu, Jūratė Kasperovičienė, Latife Köker, Kinga Kwasizur, Koray Ozhan, Valeriano Rodríguez, William Colom-Montero, Ulrike Obertegger, Micaela Vale, Spyros Gkelis, Michał Niedźwiecki, Tunay Karan, Piotr Domek, Judita Koreivienė, Andrea G. Bravo, Justyna Sieńska, Jessica Richardson, Hana Nemova, Cafer Bulut, Jordi Delgado-Martín, Tanja Žuna Pfeiffer, Marija Gligora Udovič, Manthos Panou, Dietmar Straile, Rafael Marcé, Valerie McCarthy, Iveta Drastichova, Agnieszka Napiórkowska-Krzebietke, J. A. Gálvez, Tina Elersek, Beata Messyasz, Adriano Boscaini, Carmen Ferriol, Julita Dunalska, Freshwater and Marine Ecology (IBED, FNWI), BAİBÜ, Mühendislik Fakültesi, Çevre Mühendisliği Bölümü, Karakaya, Nusret, Universitat de Barcelona, Fakülteler, Fen - Edebiyat Fakültesi, Biyoloji Bölümü, Soylu, Elif Neyran, European Cooperation in Science and Technology, Université de Genève, Tokat Gaziosmanpaşa Üniversitesi, Lammi Biological Station, Doctoral Programme in Atmospheric Sciences, CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Yılmaz, Mete, Institute of Agricultural and Environmental Sciences, Mantzouki, Evanthia, Ibelings, Bastiaan Willem, Mantzouki, E, Lurling, M, Fastner, J, Domis, LD, Wilk-Wozniak, E, Koreiviene, J, Seelen, L, Teurlincx, S, Verstijnen, Y, Krzton, W, Walusiak, E, Karosiene, J, Kasperoviciene, J, Savadova, K, Vitonyte, I, Cillero-Castro, C, Budzynska, A, Goldyn, R, Kozak, A, Rosinska, J, Szelag-Wasielewska, E, Domek, P, Jakubowska-Krepska, N, Kwasizur, K, Messyasz, B, Pelechata, A, Pelechaty, M, Kokocinski, M, Garcia-Murcia, A, Real, M, Romans, E, Noguero-Ribes, J, Duque, DP, Fernandez-Moran, E, Karakaya, N, Haggqvist, K, Demir, N, Beklioglu, M, Filiz, N, Levi, EE, Iskin, U, Bezirci, G, Tavsanoglu, UN, Ozhan, K, Gkelis, S, Panou, M, Fakioglu, O, Avagianos, C, Kaloudis, T, Celik, K, Yilmaz, M, Marce, R, Catalan, N, Bravo, AG, Buck, M, Colom-Montero, W, Mustonen, K, Pierson, D, Yang, Y, Raposeiro, PM, Goncalves, V, Antoniou, MG, Tsiarta, N, McCarthy, V, Perello, VC, Feldmann, T, Laas, A, Panksep, K, Tuvikene, L, Gagala, I, Mankiewicz-Boczek, J, Yagci, MA, Cinar, S, Capkin, K, Yagci, A, Cesur, M, Bilgin, F, Bulut, C, Uysal, R, Obertegger, U, Boscaini, A, Flaim, G, Salmaso, N, Cerasino, L, Richardson, J, Visser, PM, Verspagen, JMH, Karan, T, Soylu, EN, Maraslioglu, F, Napiorkowska-Krzebietke, A, Ochocka, A, Pasztaleniec, A, Antao-Geraldes, AM, Vasconcelos, V, Morais, J, Vale, M, Koker, L, Akcaalan, R, Albay, M, Maronic, DS, Stevic, F, Pfeiffer, TZ, Fonvielle, J, Straile, D, Rothhaupt, KO, Hansson, LA, Urrutia-Cordero, P, Blaha, L, Geris, R, Frankova, M, Kocer, MAT, Alp, MT, Remec-Rekar, S, Elersek, T, Triantis, T, Zervou, SK, Hiskia, A, Haande, S, Skjelbred, B, Madrecka, B, Nemova, H, Drastichova, I, Chomova, L, Edwards, C, Sevindik, TO, Tunca, H, Onem, B, Aleksovski, B, Krstic, S, Vucelic, IB, Nawrocka, L, Salmi, P, Machado-Vieira, D, de Oliveira, AG, Delgado-Martin, J, Garcia, D, Cereijo, JL, Goma, J, Trapote, MC, Vegas-Vilarrubia, T, Obrador, B, Grabowska, M, Karpowicz, M, Chmura, D, Ubeda, B, Galvez, JA, Ozen, A, Christoffersen, KS, Warming, TP, Kobos, J, Mazur-Marzec, H, Perez-Martinez, C, Ramos-Rodriguez, E, Arvola, L, Alcaraz-Parraga, P, Toporowska, M, Pawlik-Skowronska, B, Niedzwiecki, M, Peczula, W, Leira, M, Hernandez, A, Moreno-Ostos, E, Blanco, JM, Rodriguez, V, Montes-Perez, JJ, Palomino, RL, Rodriguez-Perez, E, Carballeira, R, Camacho, A, Picazo, A, Rochera, C, Santamans, AC, Ferriol, C, Romo, S, Soria, JM, Dunalska, J, Sienska, J, Szymanski, D, Kruk, M, Kostrzewska-Szlakowska, I, Jasser, I, Zutinic, P, Udovic, MG, Plenkovic-Moraj, A, Frak, M, Bankowska-Sobczak, A, Wasilewicz, M, Ozkan, K, Maliaka, V, Kangro, K, Grossart, HP, Paerl, HW, Carey, CC, Ibelings, BW, Sakarya Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü, Ongun Sevindik, Tuğba, Tunca, Hatice, Hitit Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, and Fen Edebiyat Fakültesi
- Subjects
light climate ,0106 biological sciences ,thermocline ,Bacterial toxins ,toksiinit ,limit of quantitation ,Toxines bacterianes ,Microcystin-LR ,Toxicology ,01 natural sciences ,Anatoxin-a ,analogs and derivatives ,BLOOMS ,Direct Effects ,uracil ,Water Pollutants ,chemistry.chemical_classification ,Temperatures ,FRESH-WATER ,latitude ,maximum buoyancy frequency ,6. Clean water ,climate change ,Indirect effects ,EUTROPHICATION ,microcystin RR ,articles ,GROWTH ,lämpötila ,LAKES ,microcystin ,anatoxin ,cylindrospermopsin ,temperature ,direct effects ,indirect effects ,spatial distribution ,European Multi Lake Survey ,epilimnetic temperature ,ta1172 ,cyanobacteria, lakes, climate warming, microcystin ,Zoology ,Article ,water pollutant ,MICROCYSTIS-AERUGINOSA ,Alkaloids ,Settore BIO/07 - ECOLOGIA ,NATURAL SCIENCES. Biology ,Spatial distribution ,Microcystis aeruginosa ,Uracil ,lake ,syanobakteerit ,Indirect Effects ,liquid chromatography-mass spectrometry ,1172 Environmental sciences ,Ekologi ,nutrient ,010604 marine biology & hydrobiology ,lcsh:R ,microbiology ,Climatic changes ,microcystin LR ,Anatoxin ,Lakes ,Spatial Distribution ,chemistry ,nodularin ,microbial diversity ,phytoplankton ,ta1181 ,Cylindrospermopsin ,Tropanes ,Cyanobacteria ,Aquatic Ecology and Water Quality Management ,analysis ,Health, Toxicology and Mutagenesis ,lcsh:Medicine ,environmental parameters ,010501 environmental sciences ,medicine.disease_cause ,nitrogen ,chemistry.chemical_compound ,sea surface temperature ,environmental factor ,ddc:550 ,Canvi climàtic ,phosphorus ,PRIRODNE ZNANOSTI. Biologija ,limit of detection ,Ecology ,Cyanobacteria Toxins ,biology ,Temperature ,levinneisyys ,Nodularin ,tropane derivative ,Europe ,DAPHNIA-MAGNA ,İndirect Effects ,Direct effects ,microbial community ,Environmental Monitoring ,high performance liquid chromatography ,Microcystins ,Climate Change ,Bacterial Toxins ,Microcystin ,välittömät oikeusvaikutukset ,cyanobacterium ,ddc:570 ,geographic distribution ,medicine ,bacterial toxin ,controlled study ,ddc:610 ,Institut für Biochemie und Biologie ,0105 earth and related environmental sciences ,nonhuman ,WIMEK ,Toxin ,longitude ,PHYTOPLANKTON ASSEMBLAGES ,Aquatic Ecology ,NITROGEN AVAILABILITY ,anatoxin a ,Aquatische Ecologie en Waterkwaliteitsbeheer ,biology.organism_classification ,Climatic change ,CLIMATE ,13. Climate action ,response variable ,Canvis climàtics - Abstract
Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains. © 2018 by the authors. Licensee MDPI, Basel, Switzerland., The authors acknowledge COST Action ES 1105 “CYANOCOST—Cyanobacterial blooms and toxins in water resources: Occurrence impacts and management” and COST Action ES 1201 “NETLAKE—Networking Lake Observatories in Europe” for contributing to this study through networking and knowledge sharing with European experts in the field. Evanthia Mantzouki was supported by a grant from the Swiss State Secretariat for Education, Research and Innovation (SERI) to Bas Ibelings and by supplementary funding from the University of Geneva. We thank Clare Ahnlund, Ena Suarez and Irene Gallego for helping out with the Swiss survey. We thank Wendy Beekman and Els J. Faassen for the nutrient and toxin analysis.
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- 2018
29. A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins
- Author
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Moritz Buck, David García, Nur Filiz, Luděk Bláha, Teresa Vegas-Vilarrúbia, Manel Leira, David Parreño Duque, Donald C. Pierson, Pablo Alcaraz-Párraga, Justyna Kobos, Bárbara Úbeda, Triantafyllos Kaloudis, Elif Neyran Soylu, Eloísa Ramos-Rodríguez, Kerstin Häggqvist, Aleksandra Pełechata, Elżbieta Szeląg-Wasielewska, Meral Apaydın Yağcı, Anna Kozak, Edward Walusiak, Meryem Beklioglu, Monserrat Real, Nusret Karakaya, Carlos Rochera, Iwona Jasser, Kristiina Mustonen, José María Blanco, Delphine Latour, Nilsun Demir, Evanthia Mantzouki, Jutta Fastner, Carmen Cillero-Castro, Danielle Machado-Vieira, Tunay Karan, Jeremy Fonvielle, Kemal Celik, Mehmet Ali Turan Koçer, Miquel Lürling, Rafael Marcé, Dietmar Straile, Valerie McCarthy, Petar Žutinić, Korhan Özkan, Özden Fakioglu, Fuat Bilgin, Maria G. Antoniou, Sarah O'Leary, Itana Bokan Vucelić, Kersti Kangro, Trine Perlt Warming, Hatice Tunca, Kirsten Christoffersen, Lidia Nawrocka, Estela Rodríguez-Pérez, Abdulkadir Yağcı, Justyna Sieńska, Jessica Richardson, Elísabeth Fernández-Morán, Beata Madrecka, Barbara Pawlik-Skowrońska, Hans-Peter Grossart, Petra M. Visser, Leonardo Cerasino, Joanna Rosińska, Ülkü Nihan Tavşanoğlu, Elvira Romans, Boris Aleksovski, Agnieszka Bańkowska-Sobczak, Pauliina Salmi, Latife Köker, Cayelan C. Carey, Magdalena Toporowska, Lauri Arvola, Antonio Picazo, Elisabeth J. Faassen, Cafer Bulut, Joana Mankiewicz-Boczek, William Colom-Montero, Gregory Giuliani, Markéta Fránková, Ulrike Obertegger, Spela Remec-Rekar, Rahmi Uysal, Tanja Žuna Pfeiffer, Sigrid Haande, Jose Luis Cereijo, Victoria Bergkemper, Eilish Beirne, Agnieszka Ochocka, Uğur Işkın, Meriç Albay, Karl-Otto Rothhaupt, Nico Salmaso, Lea Tuvikene, Roberto L. Palomino, Bastiaan Willem Ibelings, Gizem Bezirci, Carmen Pérez-Martínez, Tuğba Ongun Sevindik, Dubravka Špoljarić Maronić, Ana Maria Antão-Geraldes, Jūratė Kasperovičienė, Christos Avagianos, João Morais, Daniel Szymański, Vítor Gonçalves, Susana Romo, Jorge Juan Montes-Pérez, Kristel Panksep, J. A. Gálvez, James B. Campbell, Biel Obrador, Mehmet Cesur, Irma Vitonytė, Andrea Törökné, Hannah Cromie, Tõnu Feldmann, Eti E. Levi, Ryszard Gołdyn, Mikołaj Kokociński, Lisette N. de Senerpont Domis, Kinga Kwasizur, Burçin Önem, Michał Wasilewicz, Magdalena Grabowska, Iwona Kostrzewska-Szlakowska, Mete Yilmaz, Reyhan Akçaalan, Koray Ozhan, Jordi Noguero-Ribes, Antonio Camacho, Giovanna Flaim, Nikoletta Tsiarta, Joan Gomà, Jūratė Karosienė, Elżbieta Wilk-Woźniak, Wojciech Pęczuła, Maciej Karpowicz, Michał Niedźwiecki, Vitor Vasconcelos, Spyros Gkelis, Agnieszka Napiórkowska-Krzebietke, Laura Seelen, Juan M. Soria, Iosif Konstantinou, Kadir Çapkın, Birger Skjelbred, Agnieszka Pasztaleniec, Núria Catalán, Şakir Çinar, Anđelka Plenković-Moraj, Lucia Chomova, Mehmet Tahir Alp, Magdalena Frąk, Theodoros M. Triantis, Adriano Boscaini, Carmen Ferriol, Judita Koreivienė, Julita Dunalska, Andrea G. Bravo, Hana Nemova, Ilona Gagala, Ana García-Murcia, Mariusz Pełechaty, Enrique Moreno-Ostos, Yvon Verstijnen, Lars-Anders Hansson, Ksenija Savadova, Jolanda M. H. Verspagen, Svetislav Krstić, Anastasia Hiskia, Valeriano Rodríguez, Wojciech Krztoń, Filip Stević, Hanna Mazur-Marzec, Agnieszka Budzyńska, Faruk Maraşlıoğlu, R. Carballeira, Alo Laas, Valentini Maliaka, Micaela Vale, Piotr Domek, Jordi Delgado-Martín, Marija Gligora Udovič, Manthos Panou, Iveta Drastichova, Tina Elersek, Sven Teurlincx, Damian Chmura, Beata Messyasz, Arda Özen, Pedro M. Raposeiro, E. Emiel van Loon, Natalia Jakubowska-Krepska, Armand Hernández, Anna C. Santamans, Christine Edwards, Yang Yang, Pablo Urrutia-Cordero, Rodan Geriš, Victor C. Perello, Sevasti-Kiriaki Zervou, Hans W. Paerl, Alinne Gurjão de Oliveira, Mari Carmen Trapote, Marek Kruk, Tokat Gaziosmanpaşa Üniversitesi, Theoretical and Computational Ecology (IBED, FNWI), Freshwater and Marine Ecology (IBED, FNWI), Havran Meslek Yüksekokulu, CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Antoniou, Maria-Paraskevi, Giuliani, Gregory, Ibelings, Bastiaan Willem, European Cooperation in Science and Technology, Hernández, Armand, School of Biology, Aristotle University of Thessaloniki, Masaryk University [Brno] (MUNI), Lammi Biological Station (LBS), University of Helsinki, German Federal Environmental Agency / Umweltbundesamt (UBA), Limnological Institute, University of Konstanz, Fdn Edmund Mach, IASMA Research and Innovation Centre, Department of Biology, Faculty of Sciences, Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento em Lisboa (INESC-ID), Instituto Superior Técnico, Universidade Técnica de Lisboa (IST)-Instituto de Engenharia de Sistemas e Computadores (INESC), University of Strathclyde [Glasgow], Instituto Catalán de Investigación del Agua - ICRA (SPAIN) (ICRA), Mälardalen University (MDH), Freiburg Institute for Advanced Studies-LifeNet, Albert-Ludwigs-Universität Freiburg, Department of Computer Science and Engineering [San Diego] (CSE-UCSD), University of California [San Diego] (UC San Diego), University of California-University of California, Department of Environmental Sciences [Wageningen], Wageningen University and Research [Wageningen] (WUR), Environmental Sciences, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Universitat de Barcelona, Fen Edebiyat Fakültesi, BAİBÜ, Mühendislik Fakültesi, Çevre Mühendisliği Bölümü, Karakaya, Nusret, Hernández, Armand [0000-0001-7245-9863], Hitit Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, AKWA, Aquatic Ecology (AqE), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Department of Computer Science and Engineering [Univ California San Diego] (CSE - UC San Diego), and University of California (UC)-University of California (UC)
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Ecologia dels llacs ,Data Descriptor ,Water resources ,Aquatic Ecology and Water Quality Management ,thermocline ,010504 meteorology & atmospheric sciences ,vesien tila ,phytoplankton pigments ,010501 environmental sciences ,01 natural sciences ,Ecosystem services ,ympäristön tila ,BU Contaminants & Toxins ,Environmental monitoring ,Limnology ,lakes ,ddc:550 ,Canvi climàtic ,Geosciences, Multidisciplinary ,Survey ,ComputingMilieux_MISCELLANEOUS ,ddc:333.7-333.9 ,Climate-Change ,Europe Lakes ,Environmental resource management ,[Belirlenecek] ,Climate-change ecology ,plankton ,Eutrophication ,6. Clean water ,Computer Science Applications ,Europe ,Disparate system ,data ,international ,Bloom ,Statistics, Probability and Uncertainty ,Europa ,Environmental Monitoring ,Information Systems ,environmental variables ,Statistics and Probability ,Biological pigments ,Fitoplàncton ,Climate Change ,Cyanotoxins ,ta1172 ,BU Contaminanten & Toxines ,Climate change ,observation design ,Library and Information Sciences ,Cyanobacteria ,järvet ,Education ,European Multi Lake ,cyanotoxins ,ddc:570 ,Life Science ,Ecosystem ,14. Life underwater ,database creation objective ,syanobakteerit ,0105 earth and related environmental sciences ,WIMEK ,business.industry ,data analysis objective ,nutrient ,muuttujat ,Pigments, Biological ,15. Life on land ,Climatic changes ,dataset ,phytoplankton ,pigments ,mikrolevät ,Aquatische Ecologie en Waterkwaliteitsbeheer ,Environmental variables ,Phytoplankton pigments ,Multidisciplinär geovetenskap ,Climatic change ,Lakes ,13. Climate action ,Nutrient pollution ,Phytoplankton ,Environmental science ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,business ,Lake ecology ,Canvis climàtics ,Waters - Abstract
Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment., The authors acknowledge COST Action ES 1105 “CYANOCOST – Cyanobacterial blooms and toxins in water resources: Occurrence impacts and management” and COST Action ES 1201 “NETLAKE – Networking Lake Observatories in Europe” for contributing to this study through networking and knowledge sharing with European experts in the field. Evanthia Mantzouki was supported by a grant from the Swiss State Secretariat for Education, Research and Innovation (SERI) to Bas Ibelings and by supplementary funding from University of Geneva. We thank Wendy Beekman for the nutrient analysis and the University of Wageningen for covering the costs of this analysis from the personal funding of dr. Miquel Lürling. We thank Pieter Slot for assisting with the pigment analysis and the University of Amsterdam for covering the costs of the analysis through funding from the group of Prof. Jef Huisman and dr. Petra Visser (IBED). We would like to thank the Environmental Data Initiative for covering the cost of archiving the EMLS dataset. We would like to thank the Leibniz Institute of Freshwater Ecology and Inland Fisheries, Dept. of Experimental Limnology and the Aquatic Microbial Ecology Group for logistic and technical support of J. Fonvielle and H.-P. Grossart; and the Leibniz Association for financial support. The collection of data for Lough Erne and Lough Neagh were funded by the Department of Agriculture, Environment and Rural Affairs, Northern Ireland.
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- 2018
30. Results of 10-year monitoring of work environment contamination by cytotoxic drugs
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Lucie Bláhová, Šárka Kozáková, Luděk Bláha, and Lenka Doležalová
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Public Health, Environmental and Occupational Health - Published
- 2019
31. In vitro assessment of sex steroids and related compounds in water and sediments - a critical review
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Klára Hilscherová, Luděk Bláha, Sergio Jarque, Chimi Wangmo, and Michal Bittner
- Subjects
Geologic Sediments ,010504 meteorology & atmospheric sciences ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,Biology ,Endocrine Disruptors ,Wastewater ,01 natural sciences ,Specimen Handling ,Environmental Chemistry ,Bioassay ,Animals ,Humans ,Gonadal Steroid Hormones ,Cells, Cultured ,0105 earth and related environmental sciences ,Detection limit ,Reporter gene ,Aquatic ecosystem ,Public Health, Environmental and Occupational Health ,In vitro toxicology ,General Medicine ,In vitro ,Environmental chemistry ,Biological Assay ,Sample collection ,Water Pollutants, Chemical - Abstract
Detection of endocrine disrupting compounds in water and sediment samples has gained much importance since the evidence of their effects was reported in aquatic ecosystems in the 1990s. The aim of this review is to highlight the advances made in the field of in vitro analysis for the detection of hormonally active compounds with estrogenic, androgenic and progestogenic effects in water and sediment samples. In vitro assays have been developed from yeast, mammalian and in a few cases from fish cells. These assays are based either on the hormone-mediated proliferation of sensitive cell lines or on the hormone-mediated expression of reporter genes. In vitro assays in combination with various sample enrichment methods have been used with limits of detection as low as 0.0027 ng L-1 in water, and 0.0026 ng g-1 in sediments for estrogenicity, 0.1 ng L-1 in water, and 0.5 ng g-1 in sediments for androgenicity, and 5 ng L-1 in water for progestogenicity expressed as equivalent concentrations of standard reference compounds of 17β-estradiol, dihydrotestosterone and progesterone, respectively. The experimental results and limits of quantification, however, are influenced by the methods of sample collection, preparation, and individual laboratory practices.
- Published
- 2017
32. European demonstration program on the effect-based and chemical identification and monitoring of organic pollutants in European surface waters
- Author
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Martin Krauss, Peter Oswald, Tobias Schulze, Zsolt Tarcai, Jennifer E. Schollée, Kristin Schirmer, Andrew J. Tindall, Jaroslav Slobodnik, Manoj Sonavane, Nicolas Creusot, S. Tufi, Kevin V. Thomas, François Brion, Zuzana Toušová, Melis Muz, Marijan Ahel, Juliane Hollender, Pim E.G. Leonards, Petra Spirhanzlova, Meng Hu, Victoria Osorio Torrens, Anita O. Hidasi, Annemieke Kolkman, Xiyu Ouyang, Selim Ait-Aissa, Knut Erik Tollefsen, Marc J.-F. Suter, Werner Brack, Sanja Koprivica, Jean Froment, Carolina Di Paolo, Thomas-Benjamin Seiler, Henner Hollert, Merijn Schriks, Luděk Bláha, Marja H. Lamoree, Ana Catarina Almeida, Environmental Institute (EI), Research Centre for Toxic Compounds in the Environment [Brno] (RECETOX / MUNI), Faculty of Science [Brno] (SCI / MUNI), Masaryk University [Brno] (MUNI)-Masaryk University [Brno] (MUNI), Helmholtz Centre for Environmental Research (UFZ), RWTH Aachen University, Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG), Institut National de l'Environnement Industriel et des Risques (INERIS), Chemistry and Biology, E&H: Environmental Chemistry and Toxicology, AIMMS, E&H: Environmental Bioanalytical Chemistry, Helmholtz Zentrum für Umweltforschung = Helmholtz Centre for Environmental Research (UFZ), Rheinisch-Westfälische Technische Hochschule Aachen (RWTH), Ecole Polytechnique Fédérale de Lausanne (EPFL), Norwegian Institute for Water Research (NIVA), and WatchFrog SA [Evry]
- Subjects
Environmental Engineering ,010504 meteorology & atmospheric sciences ,adverse effects ,large volume solid phase extraction ,EDA-EMERGE ,simplified effect-directed analysis protocol ,environmental health ,human health ,[SDE.MCG]Environmental Sciences/Global Changes ,010501 environmental sciences ,01 natural sciences ,Human health ,chemistry.chemical_compound ,Algae ,SDG 3 - Good Health and Well-being ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Environmental Chemistry ,Bioassay ,14. Life underwater ,Waste Management and Disposal ,Fipronil ,0105 earth and related environmental sciences ,Pollutant ,Simplified effect-directed analysis protocol ,biology ,Chemistry ,Adverse effects ,biology.organism_classification ,Pollution ,6. Clean water ,Nonylphenol ,Large volume solid phase extraction ,Environmental health ,13. Climate action ,Environmental chemistry ,Toxicity ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Surface water - Abstract
Growing concern about the adverse environmental and human health effects of a wide range of micropollutants requires the development of novel tools and approaches to enable holistic monitoring of their occurrence, fate and effects in the aquatic environment. A European-wide demonstration program (EDP) for effect-based monitoring of micropollutants in surface waters was carried out within the Marie Curie Initial Training Network EDA-EMERGE. The main objectives of the EDP were to apply a simplified protocol for effect-directed analysis, to link biological effects to target compounds and to estimate their risk to aquatic biota. Onsite large volume solid phase extraction of 50 L of surface water was performed at 18 sampling sites in four European river basins. Extracts were subjected to effect-based analysis (toxicity to algae, fish embryo toxicity, neurotoxicity, (anti-) estrogenicity, (anti-) androgenicity, glucocorticoid activity and thyroid activity), to target analysis (151 organic micropollutants) and to nontarget screening. The most pronounced effects were estrogenicity, toxicity to algae and fish embryo toxicity. In most bioassays, major portions of the observed effects could not be explained by target compounds, especially in case of androgenicity, glucocorticoid activity and fish embryo toxicity. Estrone and nonylphenoxyacetic acid were identified as the strongest contributors to estrogenicity, while herbicides, with a minor contribution from other micropollutants, were linked to the observed toxicity to algae. Fipronil and nonylphenol were partially responsible for the fish embryo toxicity. Within the EDP, 21 target compounds were prioritized on the basis of their frequency and extent of exceedance of predicted no effect concentrations. The EDP priority list included 6 compounds, which are already addressed by European legislation, and 15 micropollutants that may be important for future monitoring of surface waters. The study presents a novel simplified protocol for effect-based monitoring and draws a comprehensive picture of the surface water status across Europe. (C) 2017 Elsevier B.V. All rights reserved.
- Published
- 2017
33. Dioxins and dioxin-like compounds in composts and digestates from European countries as determined by the in vitro bioassay and chemical analysis
- Author
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Petr Kukučka, Giulio Mariani, John P. Giesy, Bernd Manfred Gawlik, Giovanni Locoro, Luděk Bláha, Gert Suurkuusk, and Martin Beníšek
- Subjects
Environmental Engineering ,Health, Toxicology and Mutagenesis ,engineering.material ,Dioxins ,Risk Assessment ,Soil ,Cell Line, Tumor ,Hydrocarbons, Chlorinated ,Animals ,Soil Pollutants ,Environmental Chemistry ,Bioassay ,Polycyclic Aromatic Hydrocarbons ,Dioxins and dioxin-like compounds ,2. Zero hunger ,Chemistry ,Compost ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Biodegradable waste ,Contamination ,Pollution ,6. Clean water ,Humus ,Rats ,Europe ,Soil conditioner ,Anaerobic digestion ,Receptors, Aryl Hydrocarbon ,13. Climate action ,Environmental chemistry ,engineering ,Biological Assay - Abstract
Aerobic composting and anaerobic digestion plays an important role in reduction of organic waste by transforming the waste into humus, which is an excellent soil conditioner. However, applications of chemical-contaminated composts on soils may have unwanted consequences such as accumulation of persistent compounds and their transfer into food chains. The present study investigated burden of composts and digestates collected in 16 European countries (88 samples) by the compounds causing dioxin-like effects as determined by use of an in vitro transactivation assay to quantify total concentrations of aryl hydrocarbon receptor-(AhR) mediated potency. Measured concentrations of 2,3,7,8-Tetrachlorodibeno-p-dioxin (2,3,7,8-TCDD) equivalents (TEQbio) were compared to concentrations of polycyclic aromatic hydrocarbons (PAHs) and selected chlorinated compounds, including polychlorinated dibenzo-p-dioxins/furans (PCDD/ Fs), co-planar polychlorinated biphenyls (PCBs), indicator PCB congeners and organochlorine pesticides (OCPs). Median concentrations of TEQbio (dioxin-like compounds) determined by the in vitro assay in crude extracts of various types of composts ranged from 0.05 to 1.2 with a maximum 8.22 lg (TEQbio )k g 1 dry mass. Potencies were mostly associated with less persistent compounds such as PAHs because treatment with sulfuric acid removed bioactivity from most samples. The pan-European investigation of contamination by organic contaminants showed generally good quality of the composts, the majority of which were in compliance with conservative limits applied in some countries. Results demonstrate performance and added value of rapid, inexpensive, effect-based monitoring, and points out the need to derive corresponding effect-based trigger values for the risk assessment of complex contaminated matrices such as composts.
- Published
- 2015
34. Chlorination and ozonation reduce microcystin content and tumour promoting activity of complex cyanobacterial extract
- Author
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Luděk Bláha, Ondřej Adamovský, Pavel Babica, Iva Sovadinová, Brad L. Upham, Volodymyr V. Tarabara, and Alla Alpatova
- Subjects
Cyanobacteria ,Ozone ,chemistry.chemical_element ,02 engineering and technology ,Microcystin ,010501 environmental sciences ,Aquatic Science ,Oceanography ,01 natural sciences ,Toxicology ,chemistry.chemical_compound ,polycyclic compounds ,Chlorine ,Potency ,Bioassay ,Food science ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,biology ,Chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,6. Clean water ,13. Climate action ,Toxicity ,Water treatment ,0210 nano-technology - Abstract
Despite intensive research and management efforts in the past decades, cyanobacterial blooms and their toxins, such as microcystins (MCs), continue to represent a major ecological and health problem in fresh waters throughout the world. Our objective was to compare the efficacy of two commonly used drinking water treatment technologies, chlorination and ozonation, in removing MCs and in reducing tumour promotion-related effects of cyanobacteria, such as inhibition of gap junctional intercellular communication (GJIC) and activation of mitogen activated protein kinases (MAPKs) in a rat liver epithelial stem-like cell line (WB-F344). This combined chemical and bioassay approach demonstrated that ozone effectively removed all MCs from an extract of a globally important bloom-forming cyanobacterium, Microcystis sp. Ozone also significantly reduced the overall tumour promotional potency of the cyanobacterial extract, as indicated by a substantial reduction in the ability of the extract to inhibit GJIC and activate extracellular receptor kinase 1/2 (ERK1/2). Although comparable reduction of total organic carbon was achieved by ozone and chlorine treatment, chlorination was much less effective in removing MCs and reducing the effects on GJIC. Chlorination had a biphasic effect with an observed decrease of extract-induced activation of ERK1/2 at the lower chlorine doses; whereas at high doses of chlorine the by-products of chlorination actually induced the activation of ERK1/2. The extracts induced p38 activation, and chlorination was not effective in reversing this effect, while ozone did reverse this effect, albeit not as much as the activation of ERK1/2. Thus, ozone was effective in reducing the toxicity of cyanobacterial extracts while chlorination was not only lacking efficacy, but at high doses of chlorination further produced by-products that were equally toxic as the untreated samples. Our study indicates the value of using an effect-based approach to assess the efficacy of water treatment systems in removing toxins, and more specifically demonstrates that ozone was more effective at reducing the toxic potential of cyanobacterial-contaminated water.
- Published
- 2017
35. Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract
- Author
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Iva Sovadinova, Pavel Babica, Ondřej Adamovský, Alla Alpatová, Volodymyr Tarabara, Brad Luther Upham, and Luděk Bláha
- Subjects
chlorination ,microcystin ,lcsh:QH540-549.5 ,ozonation ,polycyclic compounds ,toxicity ,water treatment ,lcsh:Ecology ,Cyanobacteria - Abstract
Despite intensive research and management efforts in the past decades, cyanobacterial blooms and their toxins, such as microcystins (MCs), continue to represent a major ecological and health problem in fresh waters throughout the world. Our objective was to compare the efficacy of two commonly used drinking water treatment technologies, chlorination and ozonation, in removing MCs and in reducing tumour promotion-related effects of cyanobacteria, such as inhibition of gap junctional intercellular communication (GJIC) and activation of mitogen activated protein kinases (MAPKs) in a rat liver epithelial stem-like cell line (WB-F344). This combined chemical and bioassay approach demonstrated that ozone effectively removed all MCs from an extract of a globally important bloom-forming cyanobacterium, Microcystis sp. Ozone also significantly reduced the overall tumour promotional potency of the cyanobacterial extract, as indicated by a substantial reduction in the ability of the extract to inhibit GJIC and activate extracellular receptor kinase 1/2 (ERK1/2). Although comparable reduction of total organic carbon was achieved by ozone and chlorine treatment, chlorination was much less effective in removing MCs and reducing the effects on GJIC. Chlorination had a biphasic effect with an observed decrease of extract-induced activation of ERK1/2 at the lower chlorine doses; whereas at high doses of chlorine the by-products of chlorination actually induced the activation of ERK1/2. The extracts induced p38 activation, and chlorination was not effective in reversing this effect, while ozone did reverse this effect, albeit not as much as the activation of ERK1/2. Thus, ozone was effective in reducing the toxicity of cyanobacterial extracts while chlorination was not only lacking efficacy, but at high doses of chlorination further produced by-products that were equally toxic as the untreated samples. Our study indicates the value of using an effect-based approach to assess the efficacy of water treatment systems in removing toxins, and more specifically demonstrates that ozone was more effective at reducing the toxic potential of cyanobacterial-contaminated water.
- Published
- 2017
36. GM-011 Contamination with cytostatics in pharmacies and hospitals in our country
- Author
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L. Dolezalova, Šárka Kozáková, Lucie Bláhová, and Luděk Bláha
- Subjects
Toxicology ,Compounding ,business.industry ,Cytostatic drugs ,Medicine ,Pharmacy ,Contamination ,Health risk ,business - Abstract
Background Monitoring of contamination with cytostatics has been required by law in our country for several years. However, it was put into practice thanks to the CYTO project that was managed by our pharmacy in 2006–2010. Purpose The use of cytostatic drugs has been rising in our country. While in 2010 our pharmacy compounded 23 000 bags and syringes, this amounted to 38 000 in 2015. Therefore, regular monitoring of contamination at all work sites involved in compounding or administration is important. The level of contamination should be monitored both in the pharmacy and in the wards. Since 2007, measurement of cyclophosphamide (CP) and platinum cytostatic (Pt) contamination has been available. Since 2015, measurement of 5-fluorouracil (FU) has been also possible. These drugs belong to the most frequently used cytostatics (together they represent 49.0% of compounded units). Material and methods Sampling of surfaces is performed with unwoven textile moistened by acetate buffer. CP and FU were analysed by high performance liquid chromatography (Waters UPLC Aquity with Xevo TQ-S Agilent mass detector), and Pt were analysed by mass spectrometry with inductively coupled plasma (Agilent I CP-MS 7500). Results Higher contamination was generally found in hospital wards and compounding units. Maximal detected levels of FU on floors: hospitals, 775 pg/cm 2 (median 189.7 pg/cm 2 ); pharmacies, compounding units, 564 pg/cm 2 (median 3.6 pg/cm 2 ); and pharmacies, storage rooms, 25 pg/cm 2 (median 2 ). Maximal detected levels of CP on floors: hospitals, 3244 pg/cm 2 (median 101.9 pg/cm 2 ); pharmacies, compounding units, 638 pg/cm 2 (median 5.8 pg/cm 2 ); and pharmacies, storage rooms, 235 pg/cm 2 (median 2 ). Maximal detected levels of Pt on floors: hospitals, 5390 pg/cm 2 (median 47 pg/cm 2 ); pharmacies, compounding units, 84 pg/cm 2 (median 0.9 pg/cm 2 ); and pharmacies, storage rooms, 57 pg/cm2 (median 2 ). Conclusion Measurement of contamination raised information about the level of health risk of healthcare personnel handling cytostatics (pharmacists and nurses). Hospital pharmacists can reduce contamination on their work sites and they should co-operate with wards. Preventive measures that are able to decrease contamination in wards include branched infusion lines, wall connected infusion poles instead of stands, and self-cleaning toilet seats. References and/or acknowledgements We thank all participating hospitals. No conflict of interest
- Published
- 2017
37. Extraction of Microcystins from Animal Tissues
- Author
-
Ondřej Adamovský and Luděk Bláha
- Subjects
0106 biological sciences ,010604 marine biology & hydrobiology ,Environmental chemistry ,Nutrient pollution ,Extraction (chemistry) ,%22">Fish ,010501 environmental sciences ,Biology ,01 natural sciences ,0105 earth and related environmental sciences - Abstract
Presented are standard protocols for analyses of cyanotoxins in fresh tissues. The chapter addresss both theoretical and practical aspects of cyanobacterial toxic blooms and relevant analyses of natural toxic blooms.
- Published
- 2017
38. Lipopolysaccharide Endotoxins
- Author
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Sílvia Monteiro, Ricardo Santos, Luděk Bláha, and Geoffrey A. Codd
- Published
- 2017
39. Extraction of Cyanotoxins from Cyanobacterial Biomass
- Author
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Triantafyllos Kaloudis, Jussi Meriluoto, Luděk Bláha, Leonardo Cerasino, Shmuel Carmeli, and Hanna Mazur-Marzec
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,021110 strategic, defence & security studies ,fungi ,education ,010401 analytical chemistry ,Extraction (chemistry) ,0211 other engineering and technologies ,Environmental engineering ,nutritional and metabolic diseases ,Biomass ,02 engineering and technology ,01 natural sciences ,0104 chemical sciences ,Nutrient pollution ,Environmental chemistry ,Environmental science - Abstract
Biomass containing toxins requires various extraction approaches depending on its characteristics and dominant species. The chapter addresss both theoretical and practical aspects of cyanobacterial toxic blooms and relevant analyses of natural toxic blooms.
- Published
- 2017
40. Other Cyanobacterial Bioactive Substances
- Author
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Tina Elersek, Luděk Bláha, Shmuel Carmeli, Hanna Mazur-Marzec, and Wido Schmidt
- Subjects
Depsipeptide ,chemistry.chemical_classification ,Bioactive substance ,Endocrine disruptor ,Biochemistry ,010405 organic chemistry ,Chemistry ,Biogenic amine ,010501 environmental sciences ,01 natural sciences ,0104 chemical sciences ,0105 earth and related environmental sciences ,Cyanopeptolin - Published
- 2017
41. Analysis of Microcystins in Animal Tissues Using LC-MS/MS
- Author
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Luděk Bláha and Jiří Kohoutek
- Subjects
0303 health sciences ,Chromatography ,Microcystin YR ,030311 toxicology ,Microcystin-LR ,010501 environmental sciences ,Mass spectrometry ,01 natural sciences ,High-performance liquid chromatography ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Hplc ms ms ,Lc ms ms ,%22">Fish ,Microcystin RR ,0105 earth and related environmental sciences - Abstract
Advanced LC-MSMS methodology of MC analyses is presented. The chapter addresss both theoretical and practical aspects of cyanobacterial toxic blooms and relevant analyses of natural toxic blooms.
- Published
- 2017
42. Extraction, Purification, and Testing of LPS from Cyanobacterial Samples
- Author
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Lucie Bláhová and Luděk Bláha
- Subjects
0301 basic medicine ,congenital, hereditary, and neonatal diseases and abnormalities ,030102 biochemistry & molecular biology ,Chemistry ,fungi ,education ,nutritional and metabolic diseases ,010501 environmental sciences ,01 natural sciences ,Extraction Purification ,03 medical and health sciences ,Environmental chemistry ,human activities ,0105 earth and related environmental sciences - Abstract
This contribution provides an overview of diverse approaches of characterization of lipopolysaccharides. The chapter addresss both theoretical and practical aspects of cyanobacterial toxic blooms and relevant analyses of natural toxic blooms.
- Published
- 2017
43. Bioassay Use in the Field of Toxic Cyanobacteria
- Author
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Ana M. Cameán, Valerie Fessard, Benjamin Marie, Daniel Gutiérrez-Praena, Silvia Pichardo, Ángeles Jos, María Puerto, Andrea Törökné, Gábor Vasas, Luděk Bláha, James S. Metcalf, Bojana Žegura, Masaryk University [Brno] (MUNI), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Institute for Ethnomedicine, University of Wyoming (UW), and Wiley
- Subjects
0301 basic medicine ,Cyanobacteria ,Toxinology ,010501 environmental sciences ,cyanobacteria ,01 natural sciences ,cynabocatérie ,Limited access ,03 medical and health sciences ,test ,toxicité ,Bioassay ,Ecotoxicology ,0105 earth and related environmental sciences ,biology ,business.industry ,genotoxicity ,toxicity ,toxicologie ,biology.organism_classification ,3. Good health ,Biotechnology ,toxine ,030104 developmental biology ,bioassay ,ecotoxicitytoxin ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,Environmental chemistry ,génotoxicité ,Ecotoxicity ,business ,écotoxicologie ,Primary screening - Abstract
International audience; Numerous assessment tools based on biological units (i.e. bioassays) have been developed to complement physicochemical or immunochemical methods. Bioassays provide complex information on the overall toxic potencies or biological activities of cyanobacterial samples including isolated cyanotoxins as well as natural blooms. The present chapter provides rationale beyond the use of bioassays and shows key examples for the assessment of toxic cyanobacteria covering both the toxicology (or more specifically toxinology for natural toxins) and ecotoxicology arenas for all levels of biological organization (from in vitro to in vivo, from plants to animals). At the present time, bioassays for potential use in routine monitoring need further research and validation, and there is no clear agreement among scientists on the list of recommended bioassays. Nevertheless, bioassays play an important role in the primary screening of cyanobacteria for toxic compounds and for their detection under specific situations such as limited access to advanced instrumental analytical equipment or indications of toxic metabolites other than (or in addition to) the known cyanotoxins.
- Published
- 2017
44. Assessment of non-derivatized β-N-methylamino-l-alanine (BMAA) neurotoxin in free form in urine of patients with nonspecific neurological symptoms
- Author
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L. Kozakova, E. Kadlecova, Lucie Bláhová, Luděk Bláha, and Jiří Kohoutek
- Subjects
0301 basic medicine ,Adult ,Male ,Sample processing ,Neurotoxins ,Enzyme-Linked Immunosorbent Assay ,Urine ,Toxicology ,03 medical and health sciences ,Elisa kit ,Tandem Mass Spectrometry ,Healthy volunteers ,Neurotoxin ,Humans ,Aged ,Alanine ,Chromatography ,Cyanobacteria Toxins ,Chemistry ,Mental Disorders ,Amino Acids, Diamino ,Middle Aged ,3. Good health ,030104 developmental biology ,Free form ,Female ,Urine sample ,Chromatography, Liquid - Abstract
The beta-N-methylamino-l-alanine (BMAA) is a non-proteinogenic amino acid discussed to be produced by cyanobacteria forming harmful blooms. Since BMAA is suspected etiological agent in neurodegenerative diseases, there is a need to study and validate whether and in what concentrations can BMAA be present in human tissues. The aim of the present study was to validate analytical and extraction procedures for quantification of non-derivatized BMAA in the urine using liquid chromatography and commercial ELISA Kit. The study was focused on BMAA in different forms - dissolved, protein associated and total. The validated protocol included SPE followed by HILIC MS/MS for analyses of non-derivatized free form of BMAA with a limit of quantification 20 ng/mL. The methods for other BMAA forms (i.e. protein-associated and total) were also assessed but high matrix interferences did not allow their implementation. The method was used for analyses of free BMAA in 23 urine samples from healthy volunteers and psychiatric patients suffering from nonspecific neurological symptoms. Traces of BMAA were suspectedly detected in a single urine sample but they were not unequivocally proved according to all conservative analytical criteria. BMAA was also not confirmed in a repeatedly collected sample from the same person. The evaluated commercial BMAA ELISA Kit (Abraxis) was not suitable for determination of BMAA in extracted urine samples because of systematically highly false positive results. In agreement with recent findings, analyses of BMAA appear to methodologically challenging, and further research on BMAA in human tissues (or its precursors with potency to form BMAA under natural conditions or - eventually - during sample processing) is needed to clarify its potential ethiological role in neurodegenerative diseases.
- Published
- 2017
45. Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles
- Author
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Klára Hilscherová, Jana Lebedová, Luděk Bláha, Z. Večeřa, Marcela Buchtová, Ivan Míšek, Jiří Kohoutek, and Lucie Bláhová
- Subjects
inorganic chemicals ,medicine.disease_cause ,Biochemistry ,Mass Spectrometry ,Analytical Chemistry ,Mice ,chemistry.chemical_compound ,Liquid chromatography–mass spectrometry ,medicine ,Animals ,Derivatization ,Lung ,Inhalation exposure ,Inhalation Exposure ,Mice, Inbred ICR ,Chromatography ,Glutathione Disulfide ,Inhalation ,Chemistry ,Extraction (chemistry) ,Glutathione ,Liver ,Toxicity ,Nanoparticles ,Female ,Oxidative stress ,Cadmium ,Chromatography, Liquid - Abstract
The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The performance of the method was further demonstrated in a model experiment addressing changes in GSH and GSSG concentrations in lung of mice exposed to CdO nanoparticles during acute 72 h and chronic 13-week exposures. Inhalation exposure led to increased GSH concentrations in lung. GSSG levels were in general not affected; nonsignificant suppression occurred only after the longer 13-week period of exposure. The developed method for the sensitive detection of both GSH and GSSG in very low tissue mass enables these parameters to be studied in cases where only a little sample is available, i.e. in small organisms or in small amounts of tissue.
- Published
- 2014
46. Novel rapid in vitro cytotoxicity test on mammalian cells based on an electrochemical measuring method
- Author
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Dieter Sell, Luděk Bláha, Constanze Dürr, and Michael Pescheck
- Subjects
Chemistry ,General Chemical Engineering ,In vitro cytotoxicity ,Cell ,Nanotechnology ,Electrochemistry ,In vitro ,medicine.anatomical_structure ,Biochemistry ,Toxicity ,Materials Chemistry ,medicine ,Cytotoxic T cell ,Cytotoxicity ,IC50 - Abstract
The proliferation of pharmaceuticals and chemicals and the increasing manufacture of medical goods have made an assessment of their potential toxicological risks to man and the environment indispensable. Hence, a wide range of tests has been developed in order to generate data on the harmful effects of chemicals and pharmaceuticals on organisms. Initially, toxicological data were commonly collected from tests involving animals; however, on account of ethical objections, they are gradually being replaced by in vitro cytotoxicity tests. In this work, a new in vitro screening method for the determination of cytotoxicity, based on a novel electrochemical bioactivity sensor system, was implemented. The evaluation was based on current–time curves of a potentiostatic measurement proportional to the reduction of mediator molecules by mammalian cells. Depending on the number of reduced mediator molecules used by the cells, a current signal is produced, which provides information about the viability of living cells. By adding a toxic test substance to the cell, a reduction in the current signal can be observed, depending on the cytotoxicity of the substance. It is possible to quickly create a specific cytotoxic curve and to determine the corresponding inhibitory concentrations (IC50 value). First tests were performed on three mammalian cell lines and eight model compounds using this electrochemical measurement system. The IC50 values obtained corresponded well with the toxicity determined with an established reference cytotoxicity assay (MTT test) and with data reported in the literature.
- Published
- 2014
47. What level of estrogenic activity determined by in vitro assays in municipal waste waters can be considered as safe?
- Author
-
Klára Hilscherová, Barbora Jarošová, John P. Giesy, and Luděk Bláha
- Subjects
Municipal solid waste ,Sewage ,Estrone ,Wastewater ,Industrial waste ,chemistry.chemical_compound ,Rivers ,Water Quality ,Animals ,Humans ,Predicted no-effect concentration ,Estrenes ,lcsh:Environmental sciences ,General Environmental Science ,lcsh:GE1-350 ,business.industry ,Estrogens ,6. Clean water ,Waste treatment ,chemistry ,13. Climate action ,Environmental chemistry ,Environmental science ,Biological Assay ,Sewage treatment ,business ,Water Pollutants, Chemical ,hormones, hormone substitutes, and hormone antagonists ,Environmental Monitoring - Abstract
In vitro assays are broadly used tools to evaluate the estrogenic activity in Waste Water Treatment Plant (WWTP) effluents and their receiving rivers. Since potencies of individual estrogens to induce in vitro and in vivo responses can differ it is not possible to directly evaluate risks based on in vitro measures of estrogenic activity. Estrone, 17beta-estradiol, 17alfa-ethinylestradiol and to some extent, estriol have been shown to be responsible for the majority of in vitro estrogenic activity of municipal WWTP effluents. Therefore, in the present study safe concentrations of Estrogenic Equivalents (EEQs-SSE) in municipal WWTP effluents were derived based on simplified assumption that the steroid estrogens are responsible for all estrogenicity determined with particular in vitro assays. EEQs-SSEs were derived using the bioassay and testing protocol-specific in vitro potencies of steroid estrogens, in vivo predicted no effect concentration (PNECs) of these compounds, and their relative contributions to the overall estrogenicity detected in municipal WWTP effluents. EEQs-SSEs for 15 individual bioassays varied from 0.1 to 0.4 ng EEQ/L. The EEQs-SSEs are supposed to be increased by use of location-specific dilution factors of WWTP effluents entering receiving rivers. They are applicable to municipal wastewater and rivers close to their discharges, but not to industrial waste waters. Keywords: Estrogen, Threshold, In vitro assay, Environmental risk assessment, Waste water treatment plant
- Published
- 2014
48. Association of Surface Contamination by Antineoplastic Drugs With Different Working Conditions in Hospital Pharmacies
- Author
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Pavel Piler, Michal Oravec, Pavel Odráška, Jan Kuta, Stanislav Synek, Lenka Dolezalova, and Luděk Bláha
- Subjects
Health, Toxicology and Mutagenesis ,Antineoplastic Agents ,Toxicology ,Chemical exposure ,Tandem Mass Spectrometry ,Occupational Exposure ,Humans ,Medicine ,Hospital pharmacy ,Sample extraction ,Workplace ,Cyclophosphamide ,Inductively coupled plasma mass spectrometry ,Chromatography, High Pressure Liquid ,Czech Republic ,Platinum ,General Environmental Science ,business.industry ,Public Health, Environmental and Occupational Health ,Contamination ,Monitoring data ,Antineoplastic Drugs ,Equipment Contamination ,Pharmacy Service, Hospital ,business - Abstract
This study investigates the surface contamination levels of cyclophosphamide and platinum (a marker of platinum-containing drugs) in storage and preparation areas of hospital pharmacies and their relationship to working conditions surveyed by questionnaire. In total, 259 wipe samples were collected in 13 hospital pharmacies over 4 sampling campaigns. After sample extraction with acetate buffer, cyclophosphamide and platinum were determined using high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS) and inductively coupled plasma mass spectrometry (ICP-MS). Depending on the sampling spot and campaign, median concentrations ranged from2 to 61 pg/cm(2) and from0.2 to 6.9 pg/cm(2) for cyclophosphamide and platinum, respectively. Statistical evaluation of monitoring data revealed that the contamination level was significantly influenced by laboratory throughput (expressed as number of chemotherapies prepared per week), personnel expertise (ie, participation of pharmacists with academic education in drug admixture activities), and surface material.
- Published
- 2013
49. Novel metabolites in cyanobacterium Cylindrospermopsis raciborskii with potencies to inhibit gap junctional intercellular communication
- Author
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Martin Krauss, Ondřej Adamovský, Luděk Bláha, Kateřina Nováková, Werner Brack, and Jiří Kohoutek
- Subjects
Cyanobacteria ,Environmental Engineering ,Chemical compound ,Cell Survival ,Health, Toxicology and Mutagenesis ,Metabolite ,Bacterial Toxins ,Cell Communication ,010501 environmental sciences ,01 natural sciences ,Cell Line ,Cylindrospermopsis raciborskii ,03 medical and health sciences ,chemistry.chemical_compound ,Epidermal growth factor ,Botany ,Extracellular ,Animals ,Environmental Chemistry ,Waste Management and Disposal ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,biology ,Gap Junctions ,biology.organism_classification ,Pollution ,Rats ,chemistry ,Biochemistry ,Tumor promotion ,Signal transduction ,Cylindrospermopsis - Abstract
Despite intensive research into toxic bloom-forming cyanobacteria, the majority of their metabolites remain unknown. The present study explored in detail a novel bioactivity identified in cyanobacteria, i.e. inhibition of gap junctional intercellular communication (GJIC), a marker of tumor promotion. The extracellular mixture (exudate) of the cyanobacterial strain Cylindrospermopsis raciborskii (SAG 1.97) was fractionated by semi-preparative reversed phase HPLC, and the fractions assessed for their potencies to inhibit GJIC. Two non-polar fractions that significantly inhibited GJIC were further fractionated, tested and analyzed using multiple mass spectrometric methods. Investigations led to the identification of a putative chemical compound (molecular formula C18H34O3, m/z 299.2581 for the [M+H](+) ion) responsible for observed bioactivities. Specific inhibitors of signaling pathways were used to screen for biochemical mechanisms beyond GJIC inhibition, and the results indicate the involvement of ERK1/2 kinases via a mechanism related to the action of epidermal growth factor EGF but clearly distinct from other anthropogenic tumor promoters like polychlorinated biphenyls or polycyclic aromatic hydrocarbons. The chemical and in vitro toxicological characterizations of the newly described metabolite provide important insights into the still poorly understood health impacts of complex toxic cyanobacterial blooms and indicate that currently applied monitoring practices may underestimate actual risks.
- Published
- 2013
50. Concentrations of microcystins in tissues of several fish species from freshwater reservoirs and ponds
- Author
-
Radovan Kopp, Andrea Ziková, Jiří Kohoutek, Miroslava Palíková, Ondřej Adamovský, Stanislav Navrátil, Luděk Bláha, and Jan Mareš
- Subjects
Perch ,Silver carp ,Microcystins ,biology ,Muscles ,Fishes ,Zoology ,Fresh Water ,General Medicine ,Management, Monitoring, Policy and Law ,biology.organism_classification ,Pollution ,Grass carp ,Fishery ,Aspius aspius ,Common carp ,Predatory fish ,Liver ,Freshwater fish ,Crucian carp ,Animals ,Water Pollutants, Chemical ,Czech Republic ,Environmental Monitoring ,General Environmental Science - Abstract
The aim of this study is to summarise the determination of concentrations of microcystins (MCs) in muscle and liver of freshwater fish species caught in stagnant waters of the Czech Republic. Within the years 2007-2009, 351 muscle samples and 291 liver samples of 16 freshwater fish species derived from four fishponds, and four water reservoirs were analysed. MCs were detected in 53 liver samples. The highest concentrations of microcystins were determined in liver samples of carnivorous fish species; 50.3 ng/g of fresh weight (FW) in perch (Perca fluviatilis) and 22.7 ng/g FW in pikeperch (Sander lucioperca). MCs in liver were detected in other five fish species; asp (Aspius aspius), pike (Esox lucius), common carp (Cyprinus carpio), grass carp (Ctenopharyngodon idella) and European eel (Anguilla anguilla). Concentrations of MCs in liver of nine fish species (European bream, whitefish, tench, silver carp, European catfish, roach, chub, crucian carp and rudd) were below the detection limit of 1.2-5.4 ng/g FW for different MC congeners. However, the concentrations of MCs in all muscle samples were below the detection limit. The assessment of MCs concentrations might be influenced by the detection method used. Due to the concentrations of MCs being below the detection limit in muscle samples of all fish species analysed, it seems that there might be a low potential threat for human health in case of fish muscle consumption.
- Published
- 2013
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