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10 results on '"Lucas‐Torres, Covadonga"'

1. Metabolic NMR mapping with microgram tissue biopsy

Author

Bouzier-Sore, Anne-Karine, Lucas‐Torres, Covadonga, Roumes, Hélène, Bouchaud, Véronique, Bouzier‐Sore, Anne‐Karine, Wong, Alan, Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), ANR-16-CE11-0023,HRmicroMAS,SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS(2016), ANR-10-LABX-0039,PALM,Physics: Atoms, Light, Matter(2010), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Magnetic Resonance Spectroscopy, Microgram, [SDV]Life Sciences [q-bio], 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Slice preparation, In vivo, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, glioma, Biopsy, medicine, Animals, Radiology, Nuclear Medicine and imaging, biopsy, Rats, Wistar, Spectroscopy, ComputingMilieux_MISCELLANEOUS, medicine.diagnostic_test, Brain Neoplasms, Chemistry, Brain, Soft tissue, HR-µMAS, Nuclear magnetic resonance spectroscopy, metabolic mapping, NMR, Rats, Molecular Medicine, 030217 neurology & neurosurgery, Ex vivo, Tissue biopsy, Biomedical engineering
Abstract

International audience; This study explores the potential of profiling a microgram-scale soft tissue biopsy by NMR spectroscopy. The important elements of high resolution and high sensitivity for the spectral data are achieved through a unique probe, HR-μMAS, which allowed comprehensive profiling to be performed on microgram tissue for the first time under MAS conditions. Thorough spatially resolved metabolic maps were acquired across a coronal brain slice of rat C6 gliomas, which rendered the delineation of the tumor lesion. The results present a unique ex vivo NMR possibility to analyze tissue pathology that cannot be fully explored by the conventional approach, HR-MAS and in vivo MRS. Aside from the capability of analyzing a small localized region to track its specific metabolism, it could also offer the possibility to carry out longitudinal investigations on live animals due to the feasibility of minimally invasive tissue excision.

Published
2021

2. Characterizing extracellular diffusion properties using diffusion‐weighted MRS of sucrose injected in mouse brain

Author

Vincent, Mélissa, Gaudin, Mylène, Lucas‐torres, Covadonga, Wong, Alan, Escartin, Carole, Valette, Julien, Service MIRCEN (MIRCEN), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des Maladies Neurodégénératives - UMR 9199 (LMN), Centre National de la Recherche Scientifique (CNRS)-Service MIRCEN (MIRCEN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), ANR-11-INBS-0011,NeurATRIS,Infrastructure de Recherche Translationnelle pour les Biothérapies en Neurosciences(2011), European Project: 81826,PROJECT LactaDiff, Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, double diffusion encoding, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, brain, diffusion, apparent diffusion coefficient, tortuosity, extracellular space
Abstract

International audience; Brain water and some critically important energy metabolites, such as lactate or glucose, are present in both intracellular and extracellular spaces (ICS/ECS) at significant levels. This ubiquitous nature makes diffusion MRI/MRS data sometimes difficult to interpret and model. While it is possible to glean information on the diffusion properties in ICS by measuring the diffusion of purely intracellular endogenous metabolites (such as NAA), the absence of endogenous markers specific to ECS hampers similar analyses in this compartment. In past experiments, exogenous probes have therefore been injected into the brain to assess their apparent diffusion coefficient (ADC) and thus estimate tortuosity in ECS. Here, we use a similar approach in mice by injecting sucrose, a well-known ECS marker, in either the lateral ventricles or directly in the prefrontal cortex. For the first time, we propose a thorough characterization of ECS diffusion properties encompassing (1) short-range restriction by looking at signal attenuation at high b values, (2) tortuosity and long-range restriction by measuring ADC time-dependence at long diffusion times and (3) microscopic anisotropy by performing double diffusion encoding (DDE) measurements. Overall, sucrose diffusion behavior is strikingly different from that of intracellular metabolites. Acquisitions at high b values not only reveal faster sucrose diffusion but also some sensitivity to restriction, suggesting that the diffusion in ECS is not fully Gaussian at high b. The time evolution of the ADC at long diffusion times shows that the tortuosity regime is not reached yet in the case of sucrose, while DDE experiments suggest that it is not trapped in elongated structures. No major difference in sucrose diffusion properties is reported between the two investigated routes of injection and brain regions. These original experimental insights should be useful to better interpret and model the diffusion signal of molecules that are distributed between ICS and ECS compartments.

Published
2021

3. General Guidelines for Sample Preparation Strategies in HR-$\mu$MAS NMR-based Metabolomics of Microscopic Specimens

Author

Lucas-Torres, Covadonga, Bernard, Thierry, Huber, Gaspard, Berthault, Patrick, Nishiyama, Yusuke, Kandiyal, Pancham, Elena-Herrmann, Bénédicte, Molin, Laurent, Solari, Florence, Bouzier-Sore, Anne-Karine, Wong, Alan, Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), JEOL, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), ANR-16-CE11-0023,HRmicroMAS,SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS(2016), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)

Subjects
microscopic samples, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, sample preparation, [CHIM.MATE]Chemical Sciences/Material chemistry, metabolomics, high-resolution magic angle spinning, NMR
Abstract

International audience; The study of the metabolome within tissues, organisms, cells or biofluids can be carried out by several bioanalytical techniques. Among them, nuclear magnetic resonance (NMR) is one of the principal spectroscopic methods. This is due to a sample rotation technique, high-resolution magic angle spinning (HR-MAS), which targets the analysis of heterogeneous specimens with a bulk sample mass from 5 to 10 mg. Recently, a new approach, high-resolution micro-magic angle spinning (HR-$\mu$MAS), has been introduced. It opens, for the first time, the possibility of investigating microscopic specimens (

Published
2020

4. General Guidelines for Sample Preparation Strategies in HR-µMAS NMR-based Metabolomics of Microscopic Specimens

Author

Lucas-Torres, Covadonga, Bernard, Thierry, Huber, Gaspard, Berthault, Patrick, Nishiyama, Yusuke, Kandiyal, Pancham S., Elena-Herrmann, Bénédicte, Molin, Laurent, Solari, Florence, Bouzier-Sore, Anne-Karine, and Wong, Alan

Subjects
microscopic samples, sample preparation, lcsh:QR1-502, metabolomics, Article, high-resolution magic angle spinning, lcsh:Microbiology, nmr
Abstract

The study of the metabolome within tissues, organisms, cells or biofluids can be carried out by several bioanalytical techniques. Among them, nuclear magnetic resonance (NMR) is one of the principal spectroscopic methods. This is due to a sample rotation technique, high-resolution magic angle spinning (HR-MAS), which targets the analysis of heterogeneous specimens with a bulk sample mass from 5 to 10 mg. Recently, a new approach, high-resolution micro-magic angle spinning (HR-μMAS), has been introduced. It opens, for the first time, the possibility of investigating microscopic specimens (

Published
2020

5. Current Developments in $\mu$MAS NMR Analysis for Metabolomics

Author

Lucas-Torres, Covadonga, Wong, Alan, Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), ANR-16-CE11-0023,HRmicroMAS,SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS(2016), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
magic-angle spinning, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, metabolic profiling, HR-MAS, micro-MAS, metabolomics, NMR
Abstract

International audience; Analysis of microscopic specimens has emerged as a useful analytical application in metabolomics because of its capacity for characterizing a highly homogenous sample with a specific interest. The undeviating analysis helps to unfold the hidden activities in a bulk specimen and contributes to the understanding of the fundamental metabolisms in life. In NMR spectroscopy, micro($\mu$)-probe technology is well-established and-adopted to the microscopic level of biofluids. However, this is quite the contrary with specimens such as tissue, cell and organism. This is due to the substantial difficulty of developing a sufficient $\mu$-size magic-angle spinning (MAS) probe for sub-milligram specimens with the capability of high-quality data acquisition. It was not until 2012; a $\mu$MAS probe had emerged and shown promises to $\mu$g analysis; since, a continuous advancement has been made striving for the possibility of $\mu$MAS to be an effective NMR spectroscopic analysis. Herein, the mini-review highlights the progress of $\mu$MAS development-from an impossible scenario to an attainable solution-and describes a few demonstrative metabolic profiling studies. The review will also discuss the current challenges in $\mu$MAS NMR analysis and its potential to metabolomics.

Published
2019

6. HR-$\mu$MAS NMR localized metabolic profiling of $\mu$g samples: a model study

Author

Lucas-Torres, Covadonga, Nishiyama, Yusuke, Wong, Alan, Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), JEOL Ltd, 1156 Nakagami, Akishima, Tokyo 196-0022, Japan., ANR-16-CE11-0023,HRmicroMAS,SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS(2016), Palacin, Serge, SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS - - HRmicroMAS2016 - ANR-16-CE11-0023 - AAPG2016 - VALID, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry
Abstract

International audience; NMR has already proven to be a tremendous spectroscopic tool in metabonomics. However, due to its low detection sensitivity, NMR analyses can be challenging especially for mass-limited samples. To overcome this issue, here we introduce a 'new' reliable methodology for profiling $\mu$g/nL scale samples based on the Magic Angle Spinning (MAS) technique-High-Resolution $\mu$MAS (i.e. 1 mm HR-$\mu$MAS). A model study has been proposed: the localized metabolic profiling (i. e. mapping) of a garlic clove. We have been able to investigate 4 different regions (clove skin, flesh, core and inner epidermis), whereas only 3 regions were distinguished using the standard approach, HR-MAS with larger sample mass ($\sim$10 mg). The use of multivariate statistical analysis allowed an initial separation between the different regions by unsupervised PCA. Based on this PCA, a supervised discriminant analysis OPLS was carried out between the previously separated clusters to find the discriminant metabolites. The fact that these micro-detections have been able to offer such precise and accurate results on a model study, opens the way to new applications on heterogeneous biospecimens (such as tissues and cells), on the determination of NMR-based localized metabolic profiles to get the comprehensive fingerprint of their different anatomical regions.

Published
2018

7. Spatial metabolic NMR profiling by HR-MAS chemical shift imaging

Author

Lucas-Torres, Covadonga, Wong, Alan, Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), ANR-12-JSV5-0005,HRMACS,Développement et exploration de nouveaux micro-détecteurs RMN tournants pour des analyses métabolomiques de biopsies de petite masse(2012), ANR-16-CE11-0023,HRmicroMAS,SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS(2016), Palacin, Serge, Jeunes Chercheuses et Jeunes Chercheurs - Développement et exploration de nouveaux micro-détecteurs RMN tournants pour des analyses métabolomiques de biopsies de petite masse - - HRMACS2012 - ANR-12-JSV5-0005 - JC - VALID, SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS - - HRmicroMAS2016 - ANR-16-CE11-0023 - AAPG2016 - VALID, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, fungi, [CHIM.MATE]Chemical Sciences/Material chemistry
Abstract

International audience; HR-MAS is a valuable NMR spectroscopic tool for metabolic profiling of biospecimens. It offers high resolution NMR spectra for identifying the metabolic compositions in heterogeneous samples such as biopsies and plant tissues. However, the HR-MAS experiments only provide the metabolic profile of an entire sample without any spatial distribution of the metabolites. Combining a pulsed-field gradient (PFG) for spatial encoding with HR-MAS for chemical shift acquisition, Fayon and his team have introduced the possibility of HR-MAS chemical shift imaging (CSI) experiment for acquiring spatial metabolic profiles of a whole organism (i.e. head, body and tail), but the spectral quality is insufficient for an in-depth analysis. Here, we explore the HR-MAS CSI experiments with superior spectral quality on various specimens including biopsy and food tissues, proving the capability of a comprehensive profiling: identification, quantification and multivariate data analysis.

Published
2018

8. Introducing HR-$\mu$MAS NMR probe for $\mu$g-scale samples

Author

Lucas-Torres, Covadonga, Nishiyama, Yusuke, Wong, Alan, Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), JEOL, Palacin, Serge, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry
Abstract

International audience; NMR has already proven to be a tremendqus spectroscopic tool in metabolomics. However, due to its low detection sensitivity, NMR analyses can be challenging especially for mass-limited samples. To overcome this issue, here we introduce a 'new' reliable methodology for profiling $\mu$g/nL scale samples based on the Magic Angle Spinning (MAS) technique-High-Resolution lIMAS (HR-$\mu$MAS). HR-$\mu$MAS offers an outstanding opportunity for the detection of metabolites in $\mu$g samples with good sensitivity and high spectral resolution, which coutd widen the existing NMR-based spectroscopy to metabolomic applications and pave the path to its utilization in numerous fields of study. ln comparison to the previous approach with the spinning $\mu$-coil (HR-MACS) [1], HR-$\mu$MAS renders improvements in probe practicality, sample-preparation, data repeatability and in the flexibility of NMR experiments. Moreover, we also show the possibility of performing metabolic quantification using HR-$\mu$ MAS-important analytical component in metabolomics. The excellent NMR spectroscopic capabilities of HR-$\mu$MAS shown here for NMR metabolic profiling on $\mu$g/nL samples have demonstrated that HR-$\mu$MAS could have an impact on future NMR-based metabolomics in biological applications.

Published
2017

9. NMR-based metabolomic in study on fruit-oils: Determination of optimal treatment on fruits and differences on oil composition

Author

Lucas-Torres, Covadonga, Moreno, Andrés, Pardo, José, Alvarez-Orti, Manuel, Wong, Alan, Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM), Universidad de Castilla-La Mancha (UCLM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)

Subjects
[CHIM.MATE]Chemical Sciences/Material chemistry, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

10. Acid-free microwave-assisted hydrothermal extraction of pectin and porous cellulose from mango peel waste-towards a zero waste mango biorefinery : Towards a Zero Waste Mango Biorefinery

Author

Matharu, Avtar S., Houghton, Joseph A., Lucas-Torres, Covadonga, and Moreno, Andrés

Abstract

Mango is the second most consumed tropical fruit after banana and the by-products of mango processing (peel, kernel and seed) roughly comprise 35-60% of the total fruit weight, thus representing a potentially high volume resource of exploitable biobased chemicals and materials. Herein, conversion and characterisation of waste mango peels from three different cultivars (Alphonso, Honey and Tommy Atkins) into pectin and porous cellulose using low temperature microwave-assisted acid-free hydrolytic conditions is reported. Microwave-assisted acid-free extraction yielded up to 11.63% (dry weight basis) of pectin which was characterised by IR, NMR (both solution and solid phase) and TGA which showed close similarity to commercial (acid extracted) pectin. The degree of esterification of pectin was determined by 13C NMR (75.6-86.2%) and titrimetry (79.3-87.7%) and the pectin showed excellent gelling ability. The molecular weight as determined by GPC was in the range 14130 (Honey)-25540 (Tommy Atkins). Porosity measurements on the depectinated residue, i.e., residual cellulosic matter showed mesoporous characteristics: average pore diameter, 9.3 nm (Alphonso)-10.5 nm (Honey), however with poor surface area 16.3 m2 g-1 (Honey)-26.0 m2 g-1. Interestingly, a second microwave hydrothermal treatment on these residues retained mesoporosity whilst significantly increasing surface area (88.8 m2 g-1 (Honey)-124.0 m2 g-1 (Alphonso)) and pore volume by approximately six-fold. This is the first detailed combined study of microwave-assisted extraction to yield pectin and mesoporous cellulose towards a potential zero waste mango biorefinery.

Published
2016

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