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The fluorescence sensing sensitivity, accuracy, and photocatalytic reduction efficiency of a bcu topological Zr-MOF have been effectively improved, by firmly anchoring lanthanide Tb3+ ions in the Zr-MOF.

A convenient and efficient copper-catalyzed oxidative C3-cyanoarylation of quinoxalin-2(1H)-ones is developed using 3-aminoindazoles as efficient arylating agents via the cleavage of two C–N bonds.

A major challenge of genome-wide association studies (GWASs) is to translate phenotypic associations into biological insights. Here, we integrate a large GWAS on blood lipids involving 1.6 million individuals from five ancestries with a wide array of functional genomic datasets to discover regulatory mechanisms underlying lipid associations. We first prioritize lipid-associated genes with expression quantitative trait locus (eQTL) colocalizations and then add chromatin interaction data to narrow the search for functional genes. Polygenic enrichment analysis across 697 annotations from a host of tissues and cell types confirms the central role of the liver in lipid levels and highlights the selective enrichment of adipose-specific chromatin marks in high-density lipoprotein cholesterol and triglycerides. Overlapping transcription factor (TF) binding sites with lipid-associated loci identifies TFs relevant in lipid biology. In addition, we present an integrative framework to prioritize causal variants at GWAS loci, producing a comprehensive list of candidate causal genes and variants with multiple layers of functional evidence. We highlight two of the prioritized genes, CREBRF and RRBP1, which show convergent evidence across functional datasets supporting their roles in lipid biology. Xiang Zhu is supported by the Stein Fellowship from Stanford University and Institute for Computational and Data Sciences Seed Grant from The Pennsylvania State University. C.D.B. is supported by the NIH (R01-HL133218). Funding for the Global Lipids Genetics Consortium was provided by the NIH (R01-HL127564). This research was conducted using the UK Biobank Resource under application number 24460. This research is based on data from the Million Veteran Program, Office of Research and Development, Veterans Health Administration, and was supported by awards 2I01BX003362-03A1 and 1I01BX004821-01A1. This publication does not represent the views of the Department of Veteran Affairs or the United States Government. We thank Bethany Klunder for administrative support. Study-specific acknowledgments are provided in the supplemental information.

Neural Radiance Fields (NeRFs) have achieved great success in the past few years. However, most current methods still require intensive resources due to ray marching-based rendering. To construct urban-level radiance fields efficiently, we design Deformable Neural Mesh Primitive~(DNMP), and propose to parameterize the entire scene with such primitives. The DNMP is a flexible and compact neural variant of classic mesh representation, which enjoys both the efficiency of rasterization-based rendering and the powerful neural representation capability for photo-realistic image synthesis. Specifically, a DNMP consists of a set of connected deformable mesh vertices with paired vertex features to parameterize the geometry and radiance information of a local area. To constrain the degree of freedom for optimization and lower the storage budgets, we enforce the shape of each primitive to be decoded from a relatively low-dimensional latent space. The rendering colors are decoded from the vertex features (interpolated with rasterization) by a view-dependent MLP. The DNMP provides a new paradigm for urban-level scene representation with appealing properties: $(1)$ High-quality rendering. Our method achieves leading performance for novel view synthesis in urban scenarios. $(2)$ Low computational costs. Our representation enables fast rendering (2.07ms/1k pixels) and low peak memory usage (110MB/1k pixels). We also present a lightweight version that can run 33$\times$ faster than vanilla NeRFs, and comparable to the highly-optimized Instant-NGP (0.61 vs 0.71ms/1k pixels). Project page: \href{https://dnmp.github.io/}{https://dnmp.github.io/}., Accepted to ICCV2023

China’s Shanxi Province accounts for 12 % of global coal output, and therefore is responsible for a very large fraction of the total global methane (CH4) emissions, as well as being a large source of uncertainty due to the lack of in-situ and field measurements. This work introduces the first comprehensive attempt to compute the coal mine methane emissions (CMM) throughout Shanxi, using a mixture of bottom-up and top-down approaches. First, public and private data from 636 individual coal mines in Shanxi Province were analyzed following the IPCC Tier 2 approach, using three to five sets of observed emission factors, and rank information based on methods issued by the National Coal Mine Safety Administration and the National Energy Administration, to compile a range of bottom-up CMM on a mine-by-mine basis. An eddy-covariance tower is set up near the output flue of a well-characterized high rank coal mine in Changzhi, and used to produce an average observed CH4 flux over two two-month long periods (Winter 2021 and Autumn 2022). The observed half-hourly CH4 flux variability is found to be roughly stable over the entire observed time, and is subsequently used to produce a set of scaling factors (RATIO correction) to updating the preliminary bottom-up coal mine methane emissions to account for both bias and high-frequency temporal variabiliy. The resulting emissions dataset have been compared against commonly used global CMM datasets including EDGAR and GFEI v2, and yield three unique scientific conclusions. First, their total CH4 emissions over Shanxi lie in between this work’s 50th percentile and 70th percentile range, meaning they are slightly high. Second, both datasets have a very large amount of emissions which occur where there are no coal mines and no CH4 emitting industry, indicating that there are significant spatial disparities, with the overlapped portion of CMM emissions where mines exist consistently close to the 30th percentile of this work’s emissions, meaning they underestimate CMM in general on a mine-by-mine basis. Third, some of the mines have average emissions values which are more than the 90th percentile of the computed mine-by-mine emissions, while many are far below the 10th percentile, showing that there is a significant issue with the sampling not capturing the observed temporal variability. It is hoped that this mine-by-mine and high frequency approximation of CMM emissions can improve both top-down observation campaigns as well as provide quantitative support and identification of mitigation opportunities.

In this paper, a frequency selective surface (FSS) based shared-aperture antenna is designed for 5G/Wi-Fi applications. The 5G and Wi-Fi channel are separated to achieve different polarizations and simultaneous work. The proposed antenna is of ±45°-polarization in the 5G N79 band and vertical polarization in the Wi-Fi5.8 GHz band. The antenna is vertically stacked with the N79 band antenna located above the Wi-Fi band antenna. The N79 antenna is composed of FSS units with transmissive characteristics in the Wi-Fi band, and serves as an electromagnetically transparent surface to avoid blocking the Wi-Fi antenna. A prototype of our design is fabricated, assembled and tested, and measured results show that the prototype is able to cover the entire N79 band (4.1–5.2 GHz, 23.7%) and Wi-Fi5.8 GHz band (5.73–5.89 GHz, 2.8%). Measured average gain is 8.2 and 7.8 dBi in the N79 and Wi-Fi band, respectively, and radiation efficiency is over 86 and 80%. The proposed design exhibits separated channels, tri-polarizations, high gain and compact size, which is sufficient for regular 5G/Wi-Fi applications. The antenna also achieves a relatively wide and highly consistent signal coverage in the two bands, making it suitable for 5G/Wi-Fi multi-function communication and indoor 5G blind compensation.

PurposeThis study introduces the concept of financial advice deserts (FADs), including financial advice received from personal financial advisors (PFAs) and Certified Financial Planners™ (CFP professionals) and investigates the association between living in these FAD states and the retirement planning activities of individuals.Design/methodology/approachThis study uses merged data gathered from multiple sources including (1) available state-level information on CFP professionals from the CFP board website, (2) state-level information on PFAs from the US Bureau of Labor Statistics and (3) individual levels of retirement planning behavior and other personal characteristics from the 2018 FINRA National Financial Capability Study. Using web data extraction tools and logistic regression analyses, this study examines the association between a series of individual retirement planning activities and living in the FAD states.FindingsThe study found that living in the FAD states was negatively associated with both having retirement accounts and contributing regularly to retirement accounts. Overall, the findings of this study underscore the need for providing greater access to financial advice and improving financial literacy among financially marginalized populations who are residing in FAD states in the United States of America.Originality/valueThis study makes unique contributions to the literature by raising the issue of geographic inequality in terms of access to financial advice and introducing the innovative notion of FADs. The findings provide fresh insights into the understanding of retirement planning and preparedness from the perspective of state-level inequality of financial advice through PFAs and CFP professionals, thereby expanding the previous knowledge that emphasizes only individual- and household-level differences. Significant implications for public policies and practitioners are also discussed.

Oocyte death is a severe clinical phenotype that causes female infertility and recurrent in vitro fertilization and intracytoplasmic sperm injection failure. We aimed to identify pathogenic variants in a female infertility patient with oocyte death phenotype.Sanger sequencing was performed to screen PANX1 variants in the affected patient. Western blot analysis was used to check the effect of the variant on PANX1 glycosylation pattern in vitro.We identified a novel PANX1 variant (NM_015368.4 c.86G A, (p. Arg29Gln)) associated with the phenotype of oocyte death in a non-consanguineous family. This variant displayed an autosomal dominant inheritance pattern with reduced penetrance. Western blot analysis confirmed that the missense mutation of PANX1 (c.86G A) altered the glycosylation pattern in HeLa cells. Moreover, the mutation effects on the function of PANX1 were weaker than recently reported variants.Our findings expand the inheritance pattern of PANX1 variants to an autosomal dominant mode with reduced penetrance and enrich the variational spectrum of PANX1. These results help us to better understand the genetic basis of female infertility with oocyte death.

The continuous development of antineoplastic therapy has significantly reduced the mortality of patients with malignant tumors, but its induced cardiotoxicity has become the primary cause of long-term death in patients with malignant tumors. However, the pathogenesis of cardiotoxicity of antineoplastic therapy is currently unknown, and practical means of prevention and treatment are lacking in clinical practice. Therefore, how to effectively prevent and treat cardiotoxicity while treating tumors is a major challenge. Animal models are important tools for studying cardiotoxicity in antitumor therapy and are of great importance in elucidating pathophysiological mechanisms and developing and evaluating modality drugs. In this paper, we summarize the existing animal models in antitumor therapeutic cardiotoxicity studies and evaluate the models by observing the macroscopic signs, echocardiography, and pathological morphology of the animals, aiming to provide a reference for subsequent experimental development and clinical application.

Background Pancreatic adenocarcinoma (PAAD) is a leading cause of malignancy-related deaths worldwide, and the efficacy of immunotherapy on PAAD is limited. Studies report that long non-coding RNAs (lncRNAs) play an important role in modulating genomic instability and immunotherapy. However, the identification of genome instability-related lncRNAs and their clinical significance has not been investigated in PAAD. Methods The current study developed a computational framework for mutation hypothesis based on lncRNA expression profile and somatic mutation spectrum in pancreatic adenocarcinoma genome. We explored the potential of GInLncRNAs(genome instability-related lncRNAs) through co-expression analysis and function enrichment analysis. We further analyzed GInLncRNAs by Cox regression and used the results to construct a prognostic lncRNA signature. Finally, we analyzed the relationship between GILncSig (genomic instability derived 3-lncRNA signature) and immunotherapy. Results A GILncSig was developed using bioinformatics analyses. It could divide patients into high-risk and low-risk groups, and there was a significant difference in OS between the two groups. In addition, GILncSig was associated with genome mutation rate in pancreatic adenocarcinoma, indicating its potential value as a marker for genomic instability. The GILncSig accurately grouped wild type patients of KRAS into two risk groups. The prognosis of the low-risk group was significantly improved. GILncSig was significantly correlated with the level of immune cell infiltration and immune checkpoint. Conclusions In summary, the current study provides a basis for further studies on the role of lncRNA in genomic instability and immunotherapy. The study provides a novel method for identification of cancer biomarkers related to genomic instability and immunotherapy.

PurposeThis study aims to examine the relationship between objective and subjective aspects of financial well-being, the role of family financial support and depression symptoms of Chinese older adults.Design/methodology/approachThis study used two waves (2015 and 2018) of the Harmonized China Health and Retirement Longitudinal Study. Two financial ratios: the expenditure-to-income ratio and the financial assets ratio, were used to measure the objective aspect of financial well-being. Perceived money management difficulty was employed to measure the subjective aspect of financial well-being. Depression symptoms were measured using the Center for Epidemiologic Studies Depression Scale (CES-D) score. Three analytical models, including an ordinary least squares (OLS) model, an OLS model controlling for lagged depression and a random effects model using panel data, were used to examine the relationships between the objective and subject aspects of financial well-being and depression.FindingsThe results from the three models showed consistent relationships: the expenditure-to-income ratio was a positive contributor, while the financial assets ratio was a negative contributor to depression of older adults in China. The robustness check using binary-coded financial ratio thresholds showed that reaching the suggested thresholds was negatively associated with depression. Perceived money management difficulty contributed positively to depression. The robustness check using the fixed effects model showed no significance of the two ratios, while perceived money management difficulty was positively associated with depression. The insignificance might be due to data limitation (limited waves or rare changes across waves).Originality/valueThe findings indicate that both objective and subjective financial well-being matters in relation to depression symptoms and, therefore, to the overall mental health of the Chinese elderly. Developments in public policies are needed to promote accessible financial services, assistance programs, mental health services and facilities for the older population in China.

The TRP superfamily of channels (nomenclature as agreed by NC-IUPHAR [176, 1072]), whose founder member is the Drosophila Trp channel, exists in mammals as six families; TRPC, TRPM, TRPV, TRPA, TRPP and TRPML based on amino acid homologies. TRP subunits contain six putative TM domains and assemble as homo- or hetero-tetramers to form cation selective channels with diverse modes of activation and varied permeation properties (reviewed by [730]). Established, or potential, physiological functions of the individual members of the TRP families are discussed in detail in the recommended reviews and in a number of books [401, 686, 1155, 256]. The established, or potential, involvement of TRP channels in disease [1126] is reviewed in [448, 685], [688] and [464], together with a special edition of Biochemica et Biophysica Acta on the subject [685]. Additional disease related reviews, for pain [633], stroke [1135], sensation and inflammation [988], itch [130], and airway disease [310, 1051], are available. The pharmacology of most TRP channels has been advanced in recent years. Broad spectrum agents are listed in the tables along with more selective, or recently recognised, ligands that are flagged by the inclusion of a primary reference. See Rubaiy (2019) for a review of pharmacological tools for TRPC1/C4/C5 channels [805]. Most TRP channels are regulated by phosphoinostides such as PtIns(4,5)P2 although the effects reported are often complex, occasionally contradictory, and likely to be dependent upon experimental conditions, such as intracellular ATP levels (reviewed by [1009, 689, 801]). Such regulation is generally not included in the tables.When thermosensitivity is mentioned, it refers specifically to a high Q10 of gating, often in the range of 10-30, but does not necessarily imply that the channel's function is to act as a 'hot' or 'cold' sensor. In general, the search for TRP activators has led to many claims for temperature sensing, mechanosensation, and lipid sensing. All proteins are of course sensitive to energies of binding, mechanical force, and temperature, but the issue is whether the proposed input is within a physiologically relevant range resulting in a response. TRPA (ankyrin) familyTRPA1 is the sole mammalian member of this group (reviewed by [293]). TRPA1 activation of sensory neurons contribute to nociception [414, 890, 602]. Pungent chemicals such as mustard oil (AITC), allicin, and cinnamaldehyde activate TRPA1 by modification of free thiol groups of cysteine side chains, especially those located in its amino terminus [575, 60, 365, 577]. Alkenals with α, β-unsaturated bonds, such as propenal (acrolein), butenal (crotylaldehyde), and 2-pentenal can react with free thiols via Michael addition and can activate TRPA1. However, potency appears to weaken as carbon chain length increases [26, 60]. Covalent modification leads to sustained activation of TRPA1. Chemicals including carvacrol, menthol, and local anesthetics reversibly activate TRPA1 by non-covalent binding [424, 511, 1081, 1080]. TRPA1 is not mechanosensitive under physiological conditions, but can be activated by cold temperatures [425, 212]. The electron cryo-EM structure of TRPA1 [740] indicates that it is a 6-TM homotetramer. Each subunit of the channel contains two short ‘pore helices’ pointing into the ion selectivity filter, which is big enough to allow permeation of partially hydrated Ca2+ ions. TRPC (canonical) familyMembers of the TRPC subfamily (reviewed by [284, 778, 18, 4, 94, 446, 739, 70]) fall into the subgroups outlined below. TRPC2 is a pseudogene in humans. It is generally accepted that all TRPC channels are activated downstream of Gq/11-coupled receptors, or receptor tyrosine kinases (reviewed by [765, 953, 1072]). A comprehensive listing of G-protein coupled receptors that activate TRPC channels is given in [4]. Hetero-oligomeric complexes of TRPC channels and their association with proteins to form signalling complexes are detailed in [18] and [447]. TRPC channels have frequently been proposed to act as store-operated channels (SOCs) (or compenents of mulimeric complexes that form SOCs), activated by depletion of intracellular calcium stores (reviewed by [741, 18, 770, 820, 1121, 157, 726, 64, 158]). However, the weight of the evidence is that they are not directly gated by conventional store-operated mechanisms, as established for Stim-gated Orai channels. TRPC channels are not mechanically gated in physiologically relevant ranges of force. All members of the TRPC family are blocked by 2-APB and SKF96365 [347, 346]. Activation of TRPC channels by lipids is discussed by [70]. Important progress has been recently made in TRPC pharmacology [805, 619, 436, 102, 851, 191, 291]. TRPC channels regulate a variety of physiological functions and are implicated in many human diseases [295, 71, 885, 1031, 1025, 154, 103, 561, 913, 409]. TRPC1/C4/C5 subgroup TRPC1 alone may not form a functional ion channel [229]. TRPC4/C5 may be distinguished from other TRP channels by their potentiation by micromolar concentrations of La3+. TRPC2 is a pseudogene in humans, but in other mammals appears to be an ion channel localized to microvilli of the vomeronasal organ. It is required for normal sexual behavior in response to pheromones in mice. It may also function in the main olfactory epithelia in mice [1114, 723, 724, 1115, 539, 1168, 1109].TRPC3/C6/C7 subgroup All members are activated by diacylglycerol independent of protein kinase C stimulation [347].TRPM (melastatin) familyMembers of the TRPM subfamily (reviewed by [275, 346, 741, 1151]) fall into the five subgroups outlined below. TRPM1/M3 subgroupIn darkness, glutamate released by the photoreceptors and ON-bipolar cells binds to the metabotropic glutamate receptor 6 , leading to activation of Go . This results in the closure of TRPM1. When the photoreceptors are stimulated by light, glutamate release is reduced, and TRPM1 channels are more active, resulting in cell membrane depolarization. Human TRPM1 mutations are associated with congenital stationary night blindness (CSNB), whose patients lack rod function. TRPM1 is also found melanocytes. Isoforms of TRPM1 may present in melanocytes, melanoma, brain, and retina. In melanoma cells, TRPM1 is prevalent in highly dynamic intracellular vesicular structures [398, 708]. TRPM3 (reviewed by [714]) exists as multiple splice variants which differ significantly in their biophysical properties. TRPM3 is expressed in somatosensory neurons and may be important in development of heat hyperalgesia during inflammation (see review [941]). TRPM3 is frequently coexpressed with TRPA1 and TRPV1 in these neurons. TRPM3 is expressed in pancreatic beta cells as well as brain, pituitary gland, eye, kidney, and adipose tissue [713, 940]. TRPM3 may contribute to the detection of noxious heat [1017]. TRPM2TRPM2 is activated under conditions of oxidative stress (respiratory burst of phagocytic cells). The direct activators are calcium, adenosine diphosphate ribose (ADPR) [970] and cyclic ADPR (cADPR) [1118]. As for many ion channels, PI(4,5)P2 must also be present [1109]. Numerous splice variants of TRPM2 exist which differ in their activation mechanisms [239]. Recent studies have reported structures of human (hs) TRPM2, which demonstrate two ADPR binding sites in hsTRPM2, one in the N-terminal MHR1/2 domain and the other in the C-terminal NUDT9-H domain. In addition, one Ca2+ binding site in the intracellular S2-S3 loop is revealed and proposed to mediate Ca2+ binding that induces conformational changes leading the ADPR-bound closed channel to open [387, 1027]. Meanwhile, a quadruple-residue motif (979FGQI982) was identified as the ion selectivity filter and a gate to control ion permeation in hsTRPM2 [1120]. TRPM2 is involved in warmth sensation [848], and contributes to several diseases [76]. TRPM2 interacts with extra synaptic NMDA receptors (NMDAR) and enhances NMDAR activity in ischemic stroke [1164]. Activation of TRPM2 in macrophages promotes atherosclerosis [1165, 1147]. Moreover, silica nanoparticles induce lung inflammation in mice via ROS/PARP/TRPM2 signaling-mediated lysosome impairment and autophagy dysfunction [1028]. Recent studies have designed various compounds for their potential to selectively inhibit the TRPM2 channel, including ACA derivatives A23, and 2,3-dihydroquinazolin-4(1H)-one derivatives [1137, 1139]. TRPM4/5 subgroupTRPM4 and TRPM5 have the distinction within all TRP channels of being impermeable to Ca2+ [1072]. A splice variant of TRPM4 (i.e.TRPM4b) and TRPM5 are molecular candidates for endogenous calcium-activated cation (CAN) channels [327]. TRPM4 is active in the late phase of repolarization of the cardiac ventricular action potential. TRPM4 deletion or knockout enhances beta adrenergic-mediated inotropy [593]. Mutations are associated with conduction defects [404, 593, 879]. TRPM4 has been shown to be an important regulator of Ca2+ entry in to mast cells [993] and dendritic cell migration [52]. TRPM5 in taste receptor cells of the tongue appears essential for the transduction of sweet, amino acid and bitter stimuli [537] TRPM5 contributes to the slow afterdepolarization of layer 5 neurons in mouse prefrontal cortex [513]. Both TRPM4 and TRPM5 are required transduction of taste stimuli [246]. TRPM6/7 subgroupTRPM6 and 7 combine channel and enzymatic activities (‘chanzymes’) [172]. These channels have the unusual property of permeation by divalent (Ca2+, Mg2+, Zn2+) and monovalent cations, high single channel conductances, but overall extremely small inward conductance when expressed to the plasma membrane. They are inhibited by internal Mg2+ at ~0.6 mM, around the free level of Mg2+ in cells. Whether they contribute to Mg2+ homeostasis is a contentious issue. PIP2 is required for TRPM6 and TRPM7 activation [810, 1077]. When either gene is deleted in mice, the result is embryonic lethality [413, 1065]. The C-terminal kinase region of TRPM6 and TRPM7 is cleaved under unknown stimuli, and the kinase phosphorylates nuclear histones [479, 480]. TRPM7 is responsible for oxidant- induced Zn2+ release from intracellular vesicles [3] and contributes to intestinal mineral absorption essential for postnatal survival [622]. The putative metal transporter proteins CNNM1-4 interact with TRPM7 and regulate TRPM7 channel activity [40, 467]. TRPM8Is a channel activated by cooling and pharmacological agents evoking a ‘cool’ sensation and participates in the thermosensation of cold temperatures [63, 178, 224] reviewed by [1011, 562, 457, 649]. Direct chemical agonists include menthol and icilin[1086]. Besides, linalool can promote ERK phosphorylation in human dermal microvascular endothelial cells, down-regulate intracellular ATP levels, and activate TRPM8 [68]. Recent studies have found that TRPM8 has typical S4-S5 connectomes with clear selective filters and exowell rings [512], and have identified cryo-electron microscopy structures of mouse TRPM8 in closed, intermediate, and open states along the ligand- and PIP2-dependent gated pathways [1111]. Moreover, the last 36 amino acids at the carboxyl terminal of TRPM8 are key protein sequences for TRPM8's temperature-sensitive function [194]. TRPM8 deficiency reduced the expression of S100A9 and increased the expression of HNF4α in the liver of mice, which reduced inflammation and fibrosis progression in mice with liver fibrosis, and helped to alleviate the symptoms of bile duct disease [556]. Channel deficiency also shortens the time of hypersensitivity reactions in migraine mouse models by promoting the recovery of normal sensitivity [12]. A cyclic peptide DeC‐1.2 was designed to inhibit ligand activation of TRPM8 but not cold activation, which can eliminate the side effects of cold dysalgesia in oxaliplatin-treated mice without changing body temperature [9]. Analysis of clinical data shows that TRPM8-specific blockers WS12 can reduce tumor growth in colorectal cancer xenografted mice by reducing transcription and activation of Wnt signaling regulators and β-catenin and its target oncogenes, such as C-Myc and Cyclin D1 [732]. TRPML (mucolipin) familyThe TRPML family [782, 1132, 775, 1084, 190] consists of three mammalian members (TRPML1-3). TRPML channels are probably restricted to intracellular vesicles and mutations in the gene (MCOLN1) encoding TRPML1 (mucolipin-1) cause the neurodegenerative disorder mucolipidosis type IV (MLIV) in man. TRPML1 is a cation selective ion channel that is important for sorting/transport of endosomes in the late endocytotic pathway and specifically, fission from late endosome-lysosome hybrid vesicles and lysosomal exocytosis [822]. TRPML2 and TRPML3 show increased channel activity in low luminal sodium and/or increased luminal pH, and are activated by similar small molecules [319, 147, 877]. A naturally occurring gain of function mutation in TRPML3 (i.e. A419P) results in the varitint waddler (Va) mouse phenotype (reviewed by [782, 690]). TRPP (polycystin) familyThe TRPP family (reviewed by [216, 214, 300, 1061, 374]) or PKD2 family is comprised of PKD2 (PC2), PKD2L1 (PC2L1), PKD2L2 (PC2L2), which have been renamed TRPP1, TRPP2 and TRPP3, respectively [1072]. It should also be noted that the nomenclature of PC2 was TRPP2 in old literature. However, PC2 has been uniformed to be called TRPP2 [345]. PKD2 family channels are clearly distinct from the PKD1 family, whose function is unknown. PKD1 and PKD2 form a hetero-oligomeric complex with a 1:3 ratio. [905]. Although still being sorted out, TRPP family members appear to be 6TM spanning nonselective cation channels. TRPV (vanilloid) familyMembers of the TRPV family (reviewed by [995]) can broadly be divided into the non-selective cation channels, TRPV1-4 and the more calcium selective channels TRPV5 and TRPV6. TRPV1-V4 subfamilyTRPV1 is involved in the development of thermal hyperalgesia following inflammation and may contribute to the detection of noxius heat (reviewed by [762, 882, 922]). Numerous splice variants of TRPV1 have been described, some of which modulate the activity of TRPV1, or act in a dominant negative manner when co-expressed with TRPV1 [844]. The pharmacology of TRPV1 channels is discussed in detail in [329] and [1015]. TRPV2 is probably not a thermosensor in man [736], but has recently been implicated in innate immunity [547]. Functional TRPV2 expression is described in placental trophoblast cells of mouse [204]. TRPV3 and TRPV4 are both thermosensitive. There are claims that TRPV4 is also mechanosensitive, but this has not been established to be within a physiological range in a native environment [127, 530]. TRPV5/V6 subfamily TRPV5 and TRPV6 are highly expressed in placenta, bone, and kidney. Under physiological conditions, TRPV5 and TRPV6 are calcium selective channels involved in the absorption and reabsorption of calcium across intestinal and kidney tubule epithelia (reviewed by [1057, 205, 651, 270]).TRPV6 is reported to play a key role in calcium transport in the mouse placenta [1056].

Marine group II (MGII) archaea (Ca. Poseidoniales) are among the most abundant microbes in global oceanic surface waters and play an important role in driving marine biogeochemical cycles. Magroviruses – the viruses of MGII archaea have been recently found to occur ubiquitously in surface ocean. However, their diversity, distribution, and potential ecological functions in coastal zones especially brackish waters are unknown. Here we obtained 234 non-redundant magroviral genomes from brackish surface waters by using homology searches for viral signature proteins highlighting the uncovered vast diversity of this novel viral group. Phylogenetic analysis based on these brackish magroviruses along with previously reported marine ones identified six taxonomic groups with close evolutionary connection to both haloviruses and the viruses of Marine Group I archaea. Magroviruses were present abundantly both in brackish and open ocean samples with some showing habitat specification and others having broad spectrums of distribution between different habitats. Genome annotation suggests they may be involved in regulating multiple metabolic pathways of MGII archaea. Our results uncover the previously overlooked diversity and ecological potentials of a major archaeal virial group in global ocean and brackish waters and shed light on the cryptic evolutionary history of archaeal viruses.

This study systematically investigated the relationship between the structure properties and biological characteristics of DON in the effluents from municipal wastewater treatment plants. Ultrafiltration, FTIR spectroscopy, UV spectroscopy and EEM fluorescence spectroscopy were used to characterize the structure of organic matters in the effluent samples, and the bioavailability of DON was determined by algal/bacterial based bioassay. The quantitatively analysis of EEM spectra conducted by fluorescence regional integration method showed that the organic portion of all samples were mainly consistent with fulvic acid and protein. Combined with the bioassay results, a positive correlation between the DON bioavailability and the protein content (sum of region I and region II) (r=0.80, P＜0.02) and soluble microbial byproduct-like materials (region IV) (r=0.76, P＜0.03) were observed. Nevertheless, the humic substances content represented by the region III and V would negatively affect the DON bioavailability. High humification degree (high HIX value) (r=-0.77, P＜0.03) was related to low bioavailability. Furthermore, according to UV spectroscopy results, strong aromaticity (high UV254 values) (r=-0.78, P＜0.03) suggested low DON bioavailability. The ultrafiltration experiment showed that the low molecular weight DON (＜3kDa) accounted for 30-73% of the total DON, and no notable relationship was observed for DON molecular weight and its bioavailability.

This study used the 2015 National Financial Capability Study to investigate the relationships among financial capability, financial education, and student loan debt outcomes. Specifically, this study examines four student loan outcomes: delinquency, stress, preparation, and satisfaction among borrowers who obtained loans for themselves. Three forms of financial capability (objective financial knowledge, subjective financial knowledge, and perceived financial capability) and two forms of financial education (formal school/workplace education and informal parental education) were used as potential predictors in the study. The Probit regression results showed that expectedly, several financial capability and financial education factors were positively associated with desirable financial outcomes such as loan calculation and loan satisfaction, and negatively associated with undesirable outcomes such as loan stress and loan delinquency. However, this study also showed several unexpected results. For example, objective financial knowledge was negatively associated with loan calculation and loan satisfaction, and subjective knowledge and formal financial education were positively associated with loan delinquency.

This study used the 2017 National Financial Well-Being Survey to investigate the relationship between cognitive ability and seeking financial advice. Three aspects of cognitive ability were examined: memory, objective numeracy, and subjective numeracy. The results showed that in general, the three were not associated with seeking financial advice. However, after decomposing the sources of the advice, we found that among financial advice-seekers, memory and objective numeracy were positively associated with seeking financial advice from family. When adding the interactions between cognitive ability factors and age, older individuals with good memories were less likely to seek advice from family, while older individuals with higher objective numeracy were less likely to use social networks to seek financial advice. The study’s findings suggest future development in policies and practices to benefit those with low cognitive abilities to seek better financial advice using multiple advice sources.

The purpose of this systematic review is to evaluate for evidence of the association of acupuncture with relieving the symptoms of adenomyosis. We searched ten electronic databases and included randomized controlled trials (RCTs) in women with adenomyosis. The methodological quality was moderate evidence level by Cochrane risk-of-bias criteria. The results were analyzed by Review Manager 5.3 and expressed as standardized mean differences (SMD) or mean differences (MD). Eleven RCTs (942 subjects) were included in this meta-analysis. Analysis with no heterogeneity showed that acupuncture group obtained a significant better effect (95% CI, -0.48 to -0.10; I2 = 0%) on reducing the size of the uterus and was superior in the shrink of carbohydrate antigen 125 (CA125) level (95% CI, -1.13 to -0.44; I2 = 0%) than that in pharmacological medicine alone group. Moreover, acupuncturewas significantly associated with improving patients' dysmenorrheal symptoms after 3-month menstrual cycles treatment (95% CI, -1.25 to -0.13). The adverse events, especially the incidence of hot flashes, -1.25 to -0.13). The adverse events, especially the incidence of hot flashes, were less reported in acupuncture group compared to pharmacological medicine alone group (Odds Ratio, OR, 0.17; 95% CI, 0.08 to 0.35; I2 = 0%). Acupuncture therapy is a promising avenue for the development of alternatives to surgery and medicine in the treatment of adenomyosis. However, further rigorous trials are needed to confirm the claims of our results.

The constant emergence and rapid popularization of various intelligent technologies have brought a lot of convenience to people's lives, and also changed people's usual way of life. The use of machine automatic translation technology can greatly improve the efficiency of the analysis of publicity text information, and it is very helpful for people to deal with publicity text. The emergence of text machine automatic translation technology has brought convenience and new ideas to people's processing of large amounts of data. In the process of application, this technology will first model and analyze the semantic information contained in the text to be processed, and then output the information that people need according to their data processing requirements. In order to more clearly illustrate the effect of automatic text machine translation technology in practical applications, this paper selects two different types of text models, compares and analyzes the actual performance of this technology, and conducts a comparative study on the effect of Seq2Seq model and pre training model in translating text information. Combined with the relevant theory of deep learning, this paper illustrates the advantages and differences of the two models in translation effects, It provides scientific reference for the improvement of automatic translation model of publicity texts.

Metagenomic binning is an essential technique for genome-resolved characterization of uncultured microorganisms in various ecosystems but hampered by the low efficiency of binning tools in adequately recovering metagenome-assembled genomes (MAGs). Here, we introduce BASALT (Binning Across a Series of Assemblies Toolkit) for binning and refinement of short- and long-read sequencing data. BASALT employs multiple binners with multiple thresholds to produce initial bins, then utilizes neural networks to identify core sequences to remove redundant bins and refine non-redundant bins. Using the same assemblies generated from Critical Assessment of Metagenome Interpretation (CAMI) datasets, BASALT produced up to twice as many MAGs as VAMB, DASTool, or metaWRAP. Processing assemblies from a lake sediment dataset, BASALT produced ~30% more MAGs than metaWRAP, including 21 unique class-level prokaryotic lineages. Functional annotations revealed that BASALT could retrieve 47.6% more non-redundant opening-reading frames than metaWRAP. These results highlighted the robust handling of metagenomic sequencing data of BASALT.

HSP90α is one of the most common stress proteins in cells; hence, it is a good target for developing drugs and testing systems for cancer or physical stress levels in humans. Streptavidin conjugated quantum dots (Sav-QDs) are widely used as fluorophores for biosensing to overcome chemical labelling problems. In this work, we have attempted to develop a multifunctional and robust assay for HSP90α. The detection technique was based on the masking of the fluorescence of spotted Sav-QDs on nitrocellulose chips (NC). Biotinylated ligand/antibody attaches to the spotted Sav-QD and then HSP90α is attached, which causes the masking of fluorescence. The masking of fluorescence was used to detect protein–ligand interactions, the effect of inhibitors, protein–protein interactions, and the presence of protein in the biological sample. The load of detection (LoD) of the assay lies in the nano molar range, making it a sensitive assay. The results from the experiments suggest that the used approach is promising for developing a multifunctional, robust, and sensitive assay for proteins that can be used for point-of-care detection in complex biological samples.

Objectives The study aimed to examine long-term survival of patients with acute paraquat poisoning using computed tomography (CT) images and spirometry. Methods A total of 36 patients with long-term survival after paraquat poisoning were followed-up and divided into mild (11 patients), moderate (17 patients), and severe (8 patients) paraquat poisoning groups. Differences among the groups were compared using clinical indicators, such as peripheral capillary oxygen saturation, arterial partial pressure of oxygen and 6-min walk test (6-MWT), chest CT, spirometry, and serum immunoglobulin E (IgE). Results The 6-MWT distance was significantly shorter in the severe paraquat poisoning group than that in the mild and moderate paraquat poisoning groups. In the mild paraquat poisoning group, CT revealed no obvious lung injury, and spirometry showed normal lung function in most patients. In moderate or severe paraquat poisoning group, CT images showed fibrotic lesions as cord-like high-density shadows, reticulations, and honeycombs. In addition, other pulmonary changes, including bronchiectasis, increased lung transparency, and pulmonary bullae, were discovered. In moderate or severe paraquat poisoning group, obvious obstructive ventilation dysfunction with slight restrictive and diffuse impairment were observed in some patients, with positive bronchial relaxation test and high serum IgE level. Conclusion In the long-term follow-up, patients with severe paraquat poisoning showed the lowest exercise endurance. In moderate or severe paraquat poisoning group, CT images revealed diversified changes, not only dynamic evolution of pulmonary fibrosis process, but also signs of bronchiectasis, and chronic obstructive pulmonary disease. Some patients with moderate or severe paraquat poisoning developed obstructive ventilatory dysfunction with airway hyperresponsiveness.

Expected to be of the highest survey power telescope in the northern hemisphere, the Wide Field Survey Telescope (WFST) will begin its routine observations of the northern sky since 2023. WFST will produce a lot of scientific data to support the researches of time-domain astronomy, asteroids and the solar system, galaxy formation and cosmology and so on. We estimated that the 5 $\sigma$ limiting magnitudes of WFST with 30 second exposure are $u=22.31$ mag, $g=23.42$ mag, $r=22.95$ mag, $i=22.43$ mag, $z=21.50$ mag, $w=23.61$ mag. The above values are calculated for the conditions of $airmass=1.2$, seeing = 0.75 arcsec, precipitable water vapour (PWV) = 2.5 mm and Moon-object separation = $45^{\circ}$ at the darkest New Moon night of the Lenghu site (V=22.30 mag, Moon phase $\theta=0^{\circ}$). The limiting magnitudes in different Moon phase conditions are also calculated. The calculations are based on the empirical transmittance data of WFST optics, the vendor provided CCD quantum efficiency, the atmospherical model transmittance and spectrum of the site. In the absence of measurement data such as sky transmittance and spectrum, we use model data., Comment: Figure 5 (a,b,c) has been updated in this latest version. 12 pages, 5 figures, accepted by RAA (Research in Astronomy and Astrophysics)

Background/Aim: The aim of this study was the conception, production, material analysis and cytocompatibility analysis of a new collagen foam for medical applications. Materials and Methods: After the innovative production of various collagen sponges from bovine sources, the foams were analyzed ex vivo in terms of their structure (including pore size) and in vitro in terms of cytocompatibility according to EN ISO 10993-5/-12. In vitro, the collagen foams were compared with the established biomaterials cerabone and Jason membrane. Materials cerabone and Jason membrane. Results: Collagen foams with different compositions were successfully produced from bovine sources. Ex vivo, the foams showed a stable and long-lasting primary structure quality with a bubble area of 1,000 to 2,000 μm(2). In vitro, all foams showed sufficient cytocompatibility. Conclusion: Collagen sponges represent a promising material for hard and soft tissue regeneration. Future studies could focus on integrating and investigating different additives in the foams.

Correction to: Nature Published online 9 December 2021 In the version of this article initially published, Noha A. Yousri (Department of Genetic Medicine, Weill Cornell Medicine-Qatar, Doha, Qatar and Department of Computer and Systems Engineering, Alexandria University, Egypt) and Steven C. Hunt (Department of Internal Medicine, University of Utah, Salt Lake City, UT, USA and Department of Genetic Medicine, Weill Cornell Medicine-Qatar, Doha, Qatar) were not included in the author list. In addition, Hieab H. H. Adams (Department of Radiology and Nuclear Medicine, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands and Department of Clinical Genetics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands) was shown with an incorrect second affiliation in the HTML and PDF versions of the article. Finally, in the HTML version, Cristen J. Willer was mistakenly listed with an extra affiliation (Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), King Abdulaziz University, Jeddah, Saudi Arabia). The authors and affiliations have been corrected in the HTML and PDF versions of the article.

Additional file 1: Figure S1. The effect of pre-treatment with gallic acid (GA) on the viability of human corneal epithelial cells (HCECs) and RAW264.7 cells. The effect of GA on the viability of HCECs (a) and RAW264.7 cells (b). Data are presented as mean ± SD (n = 6). **P < 0.01, *P < 0.05 compared to the control group (0 μM gallic acid). Figure S2. Wound healing results. Control (a) and 100 μM gallic acid (b) on wound closure was visualized at 0 h post scratch of human corneal epithelial cell (HCEC) monolayer; Control (c) and 100 μM gallic acid (d) were 24 h after wound healing. e The percentage reduction of the average wound width at 6 h, 18 h and 24 h post scratch of HCEC monolayer. Data are presented as mean ± SD (n = 6). Figure S3. Gallic acid (GA) has good long-term biocompatibility. a Treatment plan and observation plan; b Ocular surface and conjunctiva images, fluorescein sodium staining of the cornea and hematoxylin and eosin (H&E) staining of the cornea after the eyes were treated with phosphate buffered saline (PBS) solution and gallic acid solution (5 mg/mL) at 10 days post instillation; c Draize Test score for ocular surface irritation over 10 days. d Intraocular pressure changes over 10 days. Data are presented as mean ± SD (n = 3). Figure S4. Representative images of the gate plot of intracellular reactive oxygen species (ROS) production in RAW264.7 (a) and human corneal epithelial cells (HCECs) (b). The production of ROS was measured by flow cytometry using the fluorescent probe 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA). SSC-A, side scatter-area; FITC-A, fluorescein isothiocyanate-area; GA, gallic acid; LPS, lipopolysaccharide. Figure S5. 5 mg/mL and 10 mg/mL gallic acid (GA) can significantly reduce corneal fluorescein sodium spotting. Corneal fluorescein representative figures (a) and staining scores (b) show the staining of the NC, EDE, EDE + 10 mg/mL GA, EDE + 5 mg/mL GA, EDE + 1 mg/mL GA groups on the fifth day after desiccant stress. The data are presented as mean ± SD (n = 3). NC, normal control, not received EDE, not given eye drops; EDE, experimental dry eye, received EDE, not given eye drops; EDE + 10 mg/mL GA, received EDE, given 10 mg/mL GA eye drops; EDE + 5 mg/mL GA, received EDE, given 5 mg/mL GA eye drops; EDE + 1 mg/mL GA, received EDE, given 1 mg/mL GA eye drops. ****P < 0.001 compared to the EDE group. Figure S6. Gallic acid (GA) can prevent corneal fluorescein sodium spot staining and cornea epithelial cell apoptosis. Corneal fluorescein representative figures (a) and staining scores (b) show the staining on the fifth day after desiccant stress. Apoptotic corneal epithelial cell count (c) and representative figures (d) show the apoptosis conditions 5 days after desiccant stress. The data are presented as mean ± SD (n = 6). NC, normal control; EDE, experimental dry eye; PBS, phosphate buffered saline; P, preventive effect of the drug; P-NC, not received EDE, not given eye drops; P-EDE: received EDE, not given eye drops; P-EDE + PBS: received EDE, given PBS eye drops; P-EDE + GA: received EDE, given GA eye drops. ****P < 0.001 compared to the P-EDE group. Figure S7. Gallic acid (GA) protects goblet cells. Goblet cells representative figures (a) and goblet cell count (b). The data are presented as mean ± SD (n = 3). NC, normal control; EDE, experimental dry eye; PBS, phosphate buffered saline; P: preventive effect of the drug; P-NC: not received EDE, not given eye drops; P-EDE: received EDE, not given eye drops; P-EDE + PBS: received EDE, given PBS eye drops; P-EDE + GA: received EDE, given GA eye drops. ***P < 0.005 compared to the P-EDE group. Figure S8. Gallic acid (GA) inhibits the elevation of inflammatory factors in the cornea and conjunctiva. a, b respectively show IL-6 and IL-1β levels per mg of the cornea. c, d respectively show IL-6 and IL-1β levels per mg of the conjunctiva. The data are presented as mean ± SD (n = 3). NC, normal control; EDE, experimental dry eye; PBS, phosphate buffered saline; P: preventive effect of the drug; P-NC: not received EDE, not given eye drops; P-EDE: received EDE, not given eye drops; P-EDE + PBS: received EDE, given PBS eye drops; P-EDE + GA: received EDE, given GA eye drops. **P < 0.01, *P < 0.05 compared to the P-EDE group.

Tuning the mass density and bulk modulus independently is the key to manipulate the propagation of sound wave. Acoustic metamaterials provide a feasible method to realize various acoustic parameters. However, the relevant studies are mainly concentrated in air, and the huge impedance difference makes it difficult to directly extend these airborne structures to underwater application. Here, we propose a metafluid to realize independent manipulation of the mass density and bulk modulus underwater. The metafluid is composed of hollow regular polygons immersed in the water. By adjusting the side number of the hollow regular polygons and choosing proper materials, the effective mass density and bulk modulus of the metafluid could be modulated independently. Based on the flexible adjustment method, metafluids with same impedance but different sound velocities are designed and used to realize an underwater impedance-matched gradient index lens. In addition, by combining the proposed metafluid with other artificial structures, acoustic parameters with great anisotropy can be achieved, which is exemplified by the design and demonstration of an impedance-matched underwater acoustic carpet cloak. This work can expand the practicability of underwater metamaterials and pave the way for future potential engineering applications in the practical underwater devices.

In recent years, there has been a significant increase in focus on the interpolation task of computer vision. Despite the tremendous advancement of video interpolation, point cloud interpolation remains insufficiently explored. Meanwhile, the existence of numerous nonlinear large motions in real-world scenarios makes the point cloud interpolation task more challenging. In light of these issues, we present NeuralPCI: an end-to-end 4D spatio-temporal Neural field for 3D Point Cloud Interpolation, which implicitly integrates multi-frame information to handle nonlinear large motions for both indoor and outdoor scenarios. Furthermore, we construct a new multi-frame point cloud interpolation dataset called NL-Drive for large nonlinear motions in autonomous driving scenes to better demonstrate the superiority of our method. Ultimately, NeuralPCI achieves state-of-the-art performance on both DHB (Dynamic Human Bodies) and NL-Drive datasets. Beyond the interpolation task, our method can be naturally extended to point cloud extrapolation, morphing, and auto-labeling, which indicates its substantial potential in other domains. Codes are available at https://github.com/ispc-lab/NeuralPCI., Comment: Accepted by CVPR 2023. Project Page: https://dyfcalid.github.io/NeuralPCI

The present study aimed at investigating the effects and mechanism of long noncoding RNA highly upregulated in metastatic triple-negative breast cancer lymph node (lncRNA HUMT) in hepatocellular carcinoma (HCC). Quantitative real-time polymerase chain reaction was used to assess the expression of HUMT, microRNA (miR)-455-5p, and low-density lipoprotein receptor-related protein 4 (LRP4) in HCC tissues. Colony forming and 5-ethynyl-2'-deoxyuridine assays were performed to assess cell proliferation. Transwell assay was performed to measure cell migration and invasion. Cell cycle distribution was assessed using flow cytometry. The protein expression of LRP4, proliferating cell nuclear antigen (PCNA), matrix metallopeptidase 2 (MMP-2), and MMP-9 was detected using western blot. Luciferase reporter assay and RNA immunoprecipitation assay was used to confirm the target association between miR-455-5p and HUMT or LRP4. In our study, the level of HUMT was enhanced in HCC tissues and cells. Cell proliferation, invasion, and migration in HCC cells were repressed by knockdown of HUMT, and knockdown of HUMT arrested cells in G1 phase and decreased the levels of PCNA, MMP-2, and MMP-9. MiR-455-5p was a target of HUMT. Lowexpression of miR-455-5p reversed the inhibitive influence on HCC cells induced by of HUMT silencing. LRP4 was a target of miR-455-5p and was negatively regulated by miR-455-5p. In addition, LRP4 expression was positively modified by HUMT, and LRP4 inhibited the inhibitory effects on HCC cells induced by HUMT silencing. In conclusion, HCC cell proliferation, invasion, and migration were restrained by knockdown of HUMT, which was related to the miR-455-5p/LRP4 axis.

Migraine is a common headache disorder. Many studies have used magnetic resonance imaging (MRI) to explore the possible pathogenesis of migraine, but they have not reached consistent conclusions and lack rigorous multiple comparison correction. Thus, this study investigates the mechanisms of migraine development from the perspective of altered functional connectivity (FC) in brain regions by using data-driven and regions of interest (ROI)-based approaches.Resting-state functional MRI data were collected from 30 patients with migraine and 40 healthy controls (HCs) matched for age, gender, and years of education. For the data-driven method, we used a voxel-mirrored homotopic connectivity (VMHC) approach to compare the FC between the patients and HCs. For the ROI-based method, significant differences in VMHC maps between the patients and HCs were defined as ROI. The seed-based approach further revealed significant differences in FC between the seeds and the other brain regions. Furthermore, the correlations between abnormal FC and clinical characteristics of patients were investigated. A rigorous multiple comparison correction was used with false discovery rate and permutation test (5000 times).In comparison with the controls group, patients showed enhanced VMHC in the bilateral thalamus. We also observed enhanced FC between the left thalamus and the left superior frontal gyrus, and increased FC between the right thalamus and the left middle frontal gyrus (Brodmann area 45 and Brodmann area 8) in patients. Further analysis showed that the FC values in the left superior frontal gyrus and left middle frontal gyrus were negatively corrected with visual analogue scale scores or attack times for headaches.Patients with migraine showed altered VMHC in the bilateral thalamus, and abnormal FC of bilateral thalamus and other brain regions. The abnormalities in thalamic FC are a likely mechanism for the development of migraine.Chinese Clinical Trial Registry, ChiCTR2000033995. Registered on 20 June 2020.

On the way to travel, the public expect to get a tourism experience with low cost, convenient travel, and high comfort. At the same time, they also have different tourism needs such as history and culture, natural landscape, and food shopping. To address the problem that traditional travel route recommendation algorithms have limited accuracy and only analyze text or pictures alone, we propose a personalized travel route recommendation algorithm that integrates text and photo information from travelogues and obtain the historical tourism footprint of tourists by analyzing travel notes. According to the frequency and cooccurrence of scenic spots in the travel notes and the number of photos taken by each scenic spot, the popularity of scenic spots and the interest preferences of various types of tourists are analyzed. Under the given starting and ending points or passing points, the optimal tourism route generation method is designed. Experiments on the real data set of Ctrip Travel website show that the recommendation accuracy of this algorithm is significantly improved compared with the traditional algorithm which only uses travel notes text or photos. Compared with the algorithm that only considers the popularity of interest points or tourists’ interest preferences, the accuracy of the route recommended by the algorithm is improved. Compared with the algorithm that only considers the cooccurrence of scenic spots or only considers the influence of photos, this algorithm can obtain a better popularity score of scenic spots. This method integrates the two kinds of information including picture and text, fully considering the interest of users with high practicability.

Base-promoted chemoselective tandem SN2′/SN2′′/D–A dimerization/elimination reaction or SN2′/SN2′′/intramolecular D–A reaction of γ-vinyl Morita–Baylis–Hillman carbonates with phenols and ortho-hydroxychalcones is reported.