1. Impairment of endometrial decidual reaction in early pregnant mice fed with high fat diet
- Author
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CHEN, Zixuan, LI, Weike, CHEN, Xuemei, LIU, Xueqing, DING, Yubin, LI, Fangfang, HE, Junlin, WANG, Yingxiong, and GAO, Rufei
- Subjects
Boron Compounds ,Male ,Estradiol ,Phalloidine ,Body Weight ,Palmitic Acid ,General Medicine ,Diet, High-Fat ,Mice, Inbred C57BL ,Endometrium ,Mice ,Cholesterol ,Homeobox A10 Proteins ,Pregnancy ,Prostaglandin-Endoperoxide Synthases ,Bone Morphogenetic Proteins ,Animals ,Female ,Collagen ,Obesity ,Azo Compounds ,Progesterone ,Triglycerides ,Research Article ,Oleic Acid - Abstract
OBJECTIVE: To investigate the effect of obesity induced by high fat diet on decidual reaction of endometrium in mice, and the effect of high fat treatment on decidual reaction of endometrial stromal cells. METHODS: Twelve 4-week-old healthy C57BL/6J female mice were randomly divided into high fat diet group and control group with 6 mice in each group. They were fed with high fat diet (22 kJ/g) or normal diet (16 kJ/g) for 12 weeks, respectively. The body weight of mice was measured every week. After feeding for 12 weeks, the body length and width of mice were measured, and the levels of fasting serum triglyceride and total cholesterol were determined. Then the mice were mated with healthy C57BL/6J male mice, and the uterine tissues were collected on the seventh day of pregnancy. The decidual cells and collagen fibers in mouse endometrium was observed by HE staining and Masson staining respectively. The expression of decidual reaction related proteins in mouse endometrium were detected by immunohistochemistry and Western blotting. Mouse endometrial stromal cells (mESCs) were isolated and treated with the oleic acid and palmitic acid in vitro , and the decidual reaction was induced with estradiol and progesterone. The accumulation of lipid droplets in mESCs was observed by oil red O and Bodipy staining. The cytoskeleton of mESCs was observed by phalloidin staining. The levels of decidual reaction related genes and proteins were detected by real-time fluorescence quantitative PCR and Western blotting. RESULTS: After feeding for 12 weeks, the body weight of mice in the high fat group was significantly higher than that in the control group ( P0.05), but the body width of mice in the high fat group was significantly larger than that in the control group ( P
- Published
- 2022