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Purpose: To determine the clinical efficacy of epirubicin plus docetaxel or paclitaxel in the management of breast cancer. Methods: This study was a randomized controlled trial that recruited 78 patients with breast cancer treated in The First People’s Hospital of Anqing between December 2018 and March 2021. The participants were randomized to receive either epirubicin plus docetaxel (control group) or epirubicin plus paclitaxel (study group), with 39 cases in each group. Clinical outcomes, adverse events, quality of life, and prognosis of the two groups were compared. Results: Epirubicin plus paclitaxel produced greater efficacy than epirubicin plus docetaxel (p < 0.05). The combination also exhibited a higher safety profile versus epirubicin with docetaxel, as evidenced by lower incidences of nausea and vomiting, intestinal discomfort, muscle pain, and dyspnea reactions (p < 0.05). Epirubicin with paclitaxel resulted in greater improvement in quality of life in patients than epirubicin plus docetaxel (p < 0.05) as well as provided more benefits in terms of survival and recurrence control than epirubicin plus docetaxel (p < 0.05). Conclusion: Epirubicin/paclitaxel combination alleviates clinical symptoms, reduces adverse events, enhances patient prognosis, lowers the risk of postoperative recurrence, and provides superior quality of life when compared to epirubicin/docetaxel combination in breast cancer patients. Nonetheless, large-scale and better-randomized clinical trials are required to validate the findings of this study.

Deep learning has been widely used in single image rain removal and demonstrated favorable universality. However, it is still challenging to remove rain streaks,especially in the nightscape rain map which exists heavy rain and rain streak accumulation. To solve this problem, a single image nightscape rain removal algorithm based on Multi-scale Fusion Residual Network is proposed in this paper. Firstly, based on the motion blur model, evenly distributed rain streaks are generated and the dataset is reconstructed to solve the lack of nightscape rain map datasets. Secondly, according to the characteristics of the night rain map, multi-scale residual blocks are drawn on to reuse and propagate the feature, so as to exploit the rain streaks details representation. Meanwhile, the linear sequential connection structure of multi-scale residual blocks is changed to a u-shaped codec structure, which tackles the problem that features cannot be extracted effectively due to insufficient scale. Finally, the features of different scales are combined with the global self-attention mechanism to get different rain streak components, then a cleaner restored image is obtained. The quantitative and qualitative results show that, compared to the existing algorithms, the proposed algorithm can effectively remove rain streaks while retaining detailed information and ensuring the integrity of image information. &nbsp

It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance. The construction of smart release nanocontainers responsive to near-infrared (NIR) laser irradiation in an on-demand and stepwise way is a promising strategy for avoiding the emergence of multidrug-resistant bacteria. Here, we develop a hydrogel composite made of alginate and nanotubes with an efficient NIR-triggered release of rifampicin and outstanding antibacterial ability. This composite hydrogel is prepared through co-encapsulating antibacterial drug (rifampicin), NIR-absorbing dye (indocyanine green), and phase-change materials (a eutectic mixture of fatty acids) into halloysite nanotubes, followed by incorporation into alginate hydrogels, allowing the in-situ gelation at room temperature and maintaining the integrity of drug-loaded nanotubes. Among them, the eutectic mixture with a melting point of 39 °C serves as the biocompatible phase-change material to facilitate the NIR-triggered drug release. The resultant phase-change material gated-nanotubes exhibit a prominent photothermal efficiency with multistep drug release under laser irradiation. In an

The complicated effects of the 2D filler characteristics on the mechanical, viscoelastic and thermal and electrical conductivity properties of polymer nanocomposites are summarized.

Lonicera japonica Thunb. has attracted much attention for its treatment of bacterial and viral infectious diseases, while its active ingredients and potential mechanisms of action have not been fully elucidated.

This paper focuses on the role of the new East Coast Rail Links (ECRL) project in Malaysia and how it will improve logistics efficiency, economic development, and environmental friendliness to meet users' expectations. The ECRL project aims to enhance connectivity between East and West Coast regions to stimulate industrial, commercial, and tourism development. The ECRL is a large-scale and heavily constructed railroad construction project. ECRL has not been a smooth project, with the government proposing the program in 2016 and construction beginning in 2017. However, due to various factors, it was suspended in 2018 and continued with a new rail route in 2019. Users' expectations for ECRL are mainly to reduce traffic congestion problems on the road, reduce high road transport costs, reduce pollution to the environment, drive business development and promote employment in the surrounding areas along the route. Because all these problems are becoming severe in Malaysia now, we will study ECRL and collect data from the respondents to point out the users' expectations of ECRL in terms of improving logistics efficiency, economic development, and environmental friendliness. After analysing the data, our study concludes with the result that all three variables positively influence users' expectations of ECRL, which, as a new railroad in Malaysia, will play a crucial role in the future development of the East Coast of Malaysia and Malaysia as a whole.

Arc volcanic rocks erupted above oceanic subduction zones generally have heavy molybdenum (Mo) isotopic compositions, which are attributed to contributions from oceanic slab–derived fluids with isotopically heavy Mo at subarc depths. Given that mafic igneous rocks with light Mo isotopic compositions have been rarely identified, it is unclear where light Mo isotope reservoirs reside, as these must exist due to oceanic subduction through geologic time. We present Mo isotope data from Mesozoic–Cenozoic mafic igneous rocks from the eastern North China craton, which were affected by continuous subduction of the oceanic slab. The Mesozoic mafic igneous rocks have island-arc basalt (IAB)–like features and high δ98Mo values of –0.15‰ to +0.09‰. In contrast, the Cenozoic mafic igneous rocks have ocean-island basalt (OIB)–like features and low δ98Mo values of –0.53‰ to –0.19‰. In addition, these two suites of rocks exhibit systematic differences in Sr-Nd isotopic compositions and fluid- and melt-mobile element contents, indicating that their mantle sources contained crustal components derived from different depths. The heavy Mo and weakly enriched Sr-Nd isotopic compositions and IAB-like trace-element signatures were inherited from slab fluids (including oceanic crust–derived aqueous solutions and seafloor sediment–derived hydrous melts) liberated at subarc depths. The light Mo and depleted Sr-Nd isotopic compositions and OIB-like trace-element signatures were inherited from dehydrated slab–derived melts (with rutile breakdown) at postarc depths. Therefore, Mo isotope systematics of mafic igneous rocks are a powerful means with which to trace the geochemical fluxes from subducting oceanic slabs to the mantle wedge at different depths.

Aims: Progression of disease within 24 months (POD24) is associated with poor survival in some subtypes of lymphoma.The aim is to identify high-risk patients with localized extranodal natural killer/T-cell lymphoma (ENKTL) and to define clinical factors associated with the risk of early recurrence after antitumor treatment. Methods: The authors retrospectively analyzed 330 cases with localized ENKTL, of which 89 experienced POD24. Results: The 5-year overall survival of the POD24 group was extremely inferior to that of the non-POD24 group. Risk factors for POD24 were Eastern Cooperative Oncology Group performance status ≥2, response evaluation (non-complete remission) after first-line treatment and elevated lactate dehydrogenase concentrations. Also, higher Epstein–Barr virus DNA titer was related to POD24. Based on these data with or without the availability of Epstein–Barr virus DNA, the authors conducted two nomograms to predict POD24, which showed good accuracy with high C statistics. Conclusion: The results showed that POD24 could serve as a marker to identify patients whose medical needs were unmet in ENKTL.

Selenium deficiency can be associated with increased susceptibility to some viral infections and even more severe diseases. In this study, we aimed to examine whether this association applies to severe fever with thrombocytopenia syndrome (SFTS).An observational study was conducted based on the data of 13,305 human SFTS cases reported in mainland China from 2010 to 2020. The associations among incidence, case fatality rate of SFTS, and crop selenium concentration at the county level were explored. The selenium level in a cohort of patients with SFTS was tested, and its relationship with clinical outcomes was evaluated.The association between selenium-deficient crops and the incidence rate of SFTS was confirmed by multivariate Poisson analysis, with an estimated incidence rate ratio (IRR, 95% confidence interval [CI]) of 4.549 (4.215-4.916) for moderate selenium-deficient counties and 16.002 (14.706-17.431) for severe selenium-deficient counties. In addition, a higher mortality rate was also observed in severe selenium-deficient counties with an IRR of 1.409 (95% CI: 1.061-1.909). A clinical study on 120 patients with SFTS showed an association between serum selenium deficiency and severe SFTS (odds ratio, OR: 2.94; 95% CI: 1.00-8.67) or fatal SFTS (OR: 7.55; 95% CI: 1.14-50.16).Selenium deficiency is associated with increased susceptibility to SFTS and poor clinical outcomes.

Quantum computing has shown considerable promise for compute-intensive tasks in recent years. For instance, classification tasks based on quantum neural networks (QNN) have garnered significant interest from researchers and have been evaluated in various scenarios. However, the majority of quantum classifiers are currently limited to binary classification tasks due to either constrained quantum computing resources or the need for intensive classical post-processing. In this paper, we propose an efficient quantum multi-classifier called MORE, which stands for measurement and correlation based variational quantum multi-classifier. MORE adopts the same variational ansatz as binary classifiers while performing multi-classification by fully utilizing the quantum information of a single readout qubit. To extract the complete information from the readout qubit, we select three observables that form the basis of a two-dimensional Hilbert space. We then use the quantum state tomography technique to reconstruct the readout state from the measurement results. Afterward, we explore the correlation between classes to determine the quantum labels for classes using the variational quantum clustering approach. Next, quantum label-based supervised learning is performed to identify the mapping between the input data and their corresponding quantum labels. Finally, the predicted label is determined by its closest quantum label when using the classifier. We implement this approach using the Qiskit Python library and evaluate it through extensive experiments on both noise-free and noisy quantum systems. Our evaluation results demonstrate that MORE, despite using a simple ansatz and limited quantum resources, achieves advanced performance., Comment: IEEE International Conference on Quantum Computing and Engineering (QCE23)

Scaling is the most commonly used technique to artificially propagate lilies. Scales from different positions of the donor bulb vary in regeneration efficiency; however, the mechanism underlying bulblet formation remains unclear. To investigate the relationship between scale morphological characteristics, initial nutrient status and bulblet regeneration capacities during scale propagation of Lilium, we performed comprehensive morphological and correlation analyses using scales from three lily cultivars. Principal component analysis clearly distinguished middle scale (MS) from outer scale (OS) by morphological characteristics alone. Morphological results indicated that MS and OS differ significantly in terms of scale width, facial area, basal area, volume, length-to-width ratio and width-to-thickness ratio. Correlation analysis showed that scale width was significantly positively correlated with both the quantity and quality of regenerated bulblets. Among the cultivars, starch and soluble sugars accounted for 50–80% of scale DW. And a higher initial ratio of sucrose to starch in scales was more conducive to the bulblets formation. Although light had no effect on the incidence of bulblets, the formation of bulblets was positively enhanced, and better morphological consistency was obtained. This present study achieved a comprehensive morphological and nutritional analysis focused on bulblet formation capacities of scales from different positions of lily bulbs via scaling propagation, laying a foundation for future molecular studies on bulblet formation.

Norovirus is a common cause of sporadic cases and outbreaks of gastroenteritis worldwide, although its prevalence and the dominant genotypes responsible for gastroenteritis outbreaks remain obscure. A systematic review was conducted on norovirus infection in China between January 2009 and March 2021. A meta-analysis and beta-binomial regression model were used to explore the epidemiological and clinical characteristics of norovirus infection and the potential factors contributing to the attack rate of the norovirus outbreaks, respectively. A total of 1132 articles with 155,865 confirmed cases were included, with a pooled positive test rate of 11.54% among 991,786 patients with acute diarrhea and a pooled attack rate of 6.73% in 500 norovirus outbreaks. GII.4 was the predominant genotype in both the etiological surveillance and outbreaks, followed by GII.3 in the etiological surveillance, and GII.17 in the outbreaks, with the proportion of recombinant genotypes increasing in recent years. A higher attack rate in the norovirus outbreaks was associated with age group (older adults), settings (nurseries, primary schools, etc.) and region (North China). The nation-wide pooled positive rate in the etiological surveillance of norovirus is lower than elsewhere in the global population, while the dominant genotypes are similar in both the etiological surveillance and the outbreak investigations. This study contributes to the understanding of norovirus infection with different genotypes in China. The prevention and control of norovirus outbreaks during the cold season should be intensified, with special attention paid to and enhanced surveillance performed in nurseries, schools and nursing homes from November to March.

Lymphoma-associated hemophagocytic syndrome (LAHS) is a rare and life-threatening clinical syndrome with rapidly deteriorating health and high mortality. We retrospectively analyzed clinical features and prognostic factors from 117 patients diagnosed with LAHS. The cumulative incidence rate of LAHS was 4.0% (117/2906). Patients were classified into B-cell LAHS (B-LAHS, n = 22) and T/natural killer (NK)-cell LAHS (T/NK-LAHS, n = 95) groups. Patients with T/NK-LAHS were younger and had lower neutrophil counts and fibrinogen values, higher LDH and transaminase levels, and were more likely to develop hemophagocytic syndrome (HPS) during the clinical course than those with B-LAHS. The median survival time for the entire cohort was 57 days, and for the T/NK-LAHS and B-LAHS groups, it was 52 and 154 days, respectively, after the diagnosis of LAHS. Patients with B-LAHS had superior 1-year OS (

Two undescribed dammarane triterpenoid saponins, cypaliurusides O and P (1 and 2), were isolated from the ethanol extracts of the leaves of Cyclocarya paliurus. Bioactivity assay results showed that compound 1 has potential cytotoxic activities against selected human cancer cell lines in vitro, with IC50 values ranging from 14.55 ± 0.55 to 22.75 ± 1.54 µM. Compound 1 showed better antitumor activity against HepG2 cells with IC50 of 14.55 ± 0.55 µM. In addition, compound 2 showed no obvious antitumor activity.

Carbonate and silicate metasomatism occurring in subduction zones is an important mechanism underlying mantle heterogeneity and compositional diversity of mantle-derived rocks. However, distinguishing between the two kinds of metasomatism is often difficult. Lithium (Li) and its isotopes have great potential in this regard because of the different Li isotopic compositions of recycled marine carbonate and silicate components. We report Li isotopic data from Cenozoic and Mesozoic alkali basalts of the West Qinling orogen in central China. Relative to those for normal basalts, very high δ7Li values (up to +11.2‰) were observed for the Cenozoic alkali basalts, but significantly and systematically lower values (as low as −3.3‰) were estimated for the Mesozoic alkali basalts. Their abnormal Li isotopic compositions, combined with major- and trace-element contents and Sr-Mg isotope ratios, indicate that the Cenozoic and Mesozoic alkali basalts originated from carbonated and silicated mantle sources, respectively, reflecting metasomatism of the mantle by slab-derived carbonate and silicate melts during Paleotethyan oceanic subduction. Interactions of such melts with the mantle peridotite in subduction channels can account for the elemental and isotopic differences of the studied alkali basalts. The present study demonstrates an effective way to distinguish between carbonate and silicate metasomatism in subduction zones by studying Li isotopic compositions of alkali basalts and highlights the prospect of Li isotopes in tracing the deep carbon cycle.

Objective. To systematically evaluate the efficacy and safety of pembrolizumab (PD-1/PD-L inhibitor) and adjuvant chemotherapy to treat NSCLC and provide evidence-based reference for clinical use. Methods. By searching the Cochrane Library, EMBASE, PubMed, and Web of Science, according to the inclusion criteria, literature selection, data extraction, and quality evaluation were carried out for the included literature. The I2 test was used to evaluate heterogeneity between studies, and the meta-analysis was performed using RevMan 5.3 software provided by Cochrane. Results. Finally, 14 relevant documents meeting the standards were included. It is a statistical difference in one-year survival rate [ OR = 1.50 , 95% CI (1.28, 1.76), P < 0.00001 , I 2 = 0 %, Z = 4.99 ]; overall response rate[OR =1.57, 95% CI (1.29, 1.90), P < 0.00001 , I 2 = 0 %, Z = 4.58 ]; progression-free survival [ OR = 2.99 , 95% CI (2.29, 3.91), P < 0.00001 , I 2 = 26 %, Z = 8.00 ]; and overall survival [ OR = 1.38 , 95% CI (1.07, 1.78), P = 0.01 , I 2 = 46 %, Z = 2.50 ] and reduces the incidence of adverse drug reactions [ OR = 2.54 , 95% CI (1.99, 3.25), P < 0.00001 , I 2 = 69 %, Z = 7.43 ]. Conclusion. Pembrolizumab adjuvant chemotherapy is effective in the treatment of advanced NSCLC, but attention should be paid to the occurrence of adverse reactions in clinical. Due to the limitations of the methodology included in the study, this conclusion required more validation of large-sample RCT.

BACKGROUND: C-X-C chemokine receptor type 4 (CXCR4) has a certain effect on renal fibrosis, and there are few specific studies in cells. We want to investigate the impact of suppressing CXCR4 activity on the expression of renal fibrosis-related genes in primary glomerular endothelial cells, mesangial cells, and podocytes. METHODS: Immunofluorescence assays were used to determine the purity of isolated glomerular endothelial cells, mesangial cells, and podocytes. CXCR4 knockdown cell lines were established by transfecting the short hairpin (sh)RNA against CXCR4. T140 and AMD3100 were used to inhibit the activity of CXCR4. LY294002 was used to inhibit the activity of phosphoinositide 3-kinase (PI3K). The mRNA expression of CXCR4 was determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The protein expression of CXCR4, collagen IV, matrix metallopeptidase (MMP)-9, PI3K, Rac1, and vascular cell adhesion protein 1 (VCAM-1) was evaluated by Western blot analysis. RESULTS: High purity was observed on isolated primary glomerular endothelial cells and podocytes. However, the purity of isolated mesangial cells was relatively low. The mRNA expression of CXCR4 was significantly suppressed by the transfection of shRNA. Compared to control cells, the expression of CXCR4, collagen IV, MMP-9, PI3K, Rac1, and VCAM-1 were dramatically downregulated in cell lines transfected with shRNA against CXCR4. Furthermore, cell lines treated with T140, AMD3100, or LY294002 also showed downregulated expression of these proteins compared to untreated cells. No significant differences were observed in the protein expression of these proteins between control cells and cells transfected with the shRNA negative control (NC). CONCLUSIONS: Suppressing the activity of CXCR4 downregulated the expression of renal fibrosis-related genes in primary glomerular cells, even under a non-inflammatory state.

BACKGROUND: To explore the development of central nervous system (CNS) symptoms and clinical application in predicting the clinical outcomes of SARS-COV-2 patients. METHODS: A retrospective cohort study was performed on the hospitalized patients with SARS-COV-2 recruited from four hospitals in Hubei Province, China from 18 January to 10 March 2020. The patients with CNS symptoms were determined. Data regarding clinical symptoms and laboratory tests were collected from medical records. RESULTS: Of 1268 patients studied, 162 (12.8%) had CNS symptoms, manifested as unconsciousness (71, 5.6%), coma (69, 5.4%), dysphoria (50, 3.9%), somnolence (34, 2.7%) and convulsion (3, 0.2%), which were observed at median of 14 (interquartile range 9-18) days after symptom onset and significantly associated with older age (OR = 5.71, 95% confidence interval [CI] 2.78-11.73), male (OR = 1.73, 95% CI 1.22-2.47) and preexisting hypertension (OR = 1.78, 95% CI 1.23-2.57). The presence of CNS symptoms could be predicted by abnormal laboratory tests across various clinical stages, including by lymphocyte counts of

Post-collisional alkaline intrusive rocks from the Dabie orogen were studied for their whole-rock major-trace elements and Sr-Nd-Hf-Pb isotopes, as well as zircon U-Pb ages and Hf-O isotopes. The results provide geochemical constraints on the nature of their mantle sources and thus insight into crust-mantle interaction in the continental collision zone. The alkaline intrusive rocks are composed of syenite and nepheline syenite. Syn-magmatic zircon U-Pb dating by LA-ICP-MS for them yielded Early Cretaceous ages of 131.3 ± 1.4 Ma to 122.6 ± 0.6 Ma, coeval with the post-collisional magmatism in the Dabie orogen. One relict zircon with U-Pb age of 211 Ma is consistent with the timing of metamorphism for the ultrahigh-pressure (UHP) metamorphic rocks in this orogen. They have arc-like trace element distribution patterns, such as enrichment in LILE (large ion lithophile element) and LREE (light rare earth element) but depletion in HFSE (high field strength element), and enriched whole-rock Sr-Nd-Hf isotope compositions with high (87Sr/86Sr)i ratios of 0.7077–0.7131 but negative εNd(t) values of −16.0 to −9.4 and εHf(t) values of −17.5 to −12.7. Their syn-magmatic zircons have three groups of Hf-O isotope compositions comparable to those of UHP metamorphic rocks in Central-South Dabie and North Dabie, which represent the upper and middle continental crust of the subducted South China Block, respectively. In this regard, slab–mantle interaction is evident during the Triassic continental collision. We suggest that the melts derived from the subducted South China Block reacted with the lithospheric mantle wedge of the North China Block, resulting in phlogopite-bearing metasomatites, whose partial melting would generate the post-collisional alkaline intrusive rocks during the Early Cretaceous.

Eight Gram-negative, aerobic, motile with paired polar flagella and rod-shaped bacteria were isolated from six tobacco fields in Yunnan, PR China. 16S rRNA gene sequence analysis revealed that all the strains belonged to the genus Ralstonia. Among them, strain 22TCCZM03-6 had an identical 16S rRNA sequence to that of R. wenshanensis 56D2T, and the other strains were closely related to R. pickettii DSM 6297T (98.34–99.86%), R. wenshanensis 56D2T (98.70–99.64%), and R. insidiosa CCUG 46789T (97.34–98.56%). Genome sequencing yielded sizes ranging from 5.17 to 5.72 Mb, with overall G + C contents of 63.3–64.1%. Pairwise genome comparisons showed that strain 22TCCZM03-6 shared average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values above the species cut-off with R. wenshanensis 56D2T, suggesting that strain 22TCCZM03-6 is a special strain of the R. wenshanensis. Five strains, including 21MJYT02-10T, 21LDWP02-16, 22TCJT01-1, 22TCCZM01-4, and 22TCJT01-2, had ANI values >95% and dDDH values >70% when compared with each other. These five strains had ANI values of 73.32–94.17% and dDDH of 22.0–55.20% with the type strains of the genus Ralstonia individually, supporting these five strains as a novel species in the genus Ralstonia. In addition, strains 21YRMH01-3T and 21MJYT02-11T represent two independent species. They both had ANI and dDDH values below the thresholds for species delineation when compared with the type species of the genus Ralstonia. In strains 21YRMH01-3T and 21MJYT02-10T, the main fatty acids were summed features 3, 8, and C16:0; however, strain 21MJYT02-11T contained C16:0, cyclo-C17:0, and summed features 3 as major fatty acids. The main polar lipids, including diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, were identified from strains 21YRMH01-3T, 21MJYT02-10T, and 21MJYT02-11T. The ubiquinones Q-7 and Q-8 were also detected in these strains, with Q-8 being the predominant quinone. Based on the above data, we propose that the eight strains represent one known species and three novel species in the genus Ralstonia, for which the names Ralstonia chuxiongensis sp. nov., Ralstonia mojiangensis sp. nov., and Ralstonia soli sp. nov. are proposed. The type strains are 21YRMH01-3T (=GDMCC 1.3534T = JCM 35818T), 21MJYT02-10T (=GDMCC 1.3531T = JCM 35816T), and 21MJYT02-11T (=GDMCC 1.3532T = JCM 35817T), respectively.

Background Surgical repair is indicated for patellar tendon ruptures that result in loss of knee extensor mechanism function. However, biomechanical studies report conflicting results when comparing transosseous suture versus suture anchor repair techniques. This discrepancy may be due to inconsistencies in experimental design as these studies use various numbers of suture strands. Therefore, the main objective of this study is to compare the ultimate load of four- versus six-strand transosseous suture repair. Secondary objectives are to compare gap formation after cyclical loading and mode of failure. Methods Six pairs of fresh-frozen cadaveric specimen were randomly allocated to either four- or six-strand transosseous suture repair. Specimen underwent preconditioning cyclical loading and then load to failure. Results The six-strand repair had a significantly higher maximum load to failure compared with the four-strand repair (mean difference = 319.3 N [57.9%], p = 0.03). There was no significant difference in gap length after cyclical loading or at max load. There were no significant differences in mode of failure. Conclusion Utilizing a six-stand transosseous patella tendon repair construct with one additional suture increases overall construct strength by over 50% compared with a four-strand construct.

Supplementary Table S1. Characteristics of the study population