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Additional file 1: Supplement 1. Risk stratification based on the C-HUANAN-AML15 protocol.

This paper reports a longitudinal study on the retrospective evaluation of perceived usability. We propose a retest method based on SUS questionnaires to reduce the multiple-observation effects typically present in longitudinal research. We designed different forms of the SUS questionnaire to eliminate memory effects and distributed duplicate SUS copies to subjects. We first measured the alternative-form reliability of duplicate in Experiment I ($\textbf{r =0.814}$). We then found that the duplicates we designed significantly reduce memory effects compared to the standard SUS questionnaire. Experiment II involved tests on the retrospective evaluation of perceived usability at five measurement points after initial use. The retrospective evaluation of perceived usability changes over time. After 5 min (at point ${\mathrm{T}}_2$), we found that each subject’s retrospective evaluation changed significantly regardless of whether the task had higher or lower usability levels. Moreover, the `directions’of this change tended to differ. This indicates that time is an important factor in perceived usability evaluation. Our results altogether indicate that time affects the retrospective evaluation of perceived usability as well as the accuracy of perceived usability evaluation, which suggests that the process of perceived usability assessment requires strict time constraints.

Objective The present study is to evaluate the biological functions of long non-coding RNA (lncRNA), X-inactive specific transcript, X-inactive specific transcript (XIST) in human epithelial ovarian cancer (EOC). Methods XIST was upregulated in EOC cell lines, CAOV3 and OVCAR3 cells by lentiviral transduction. The effects of XIST overexpression on cancer cell proliferation, invasion, chemosensitivity and in vivo tumor growth were investigated, respectively. Possible sponging interaction between XIST and human microRNA hsa-miR-214-3p was further evaluated. Furthermore, hsa-miR-214-3p was overexpressed in XIST-upregulated CAOV3 and OVCAR3 cells to evaluate its effect on XIST-mediated EOC regulation. Results Lentivirus-mediated XIST upregulation had significant anticancer effects in CAOV3 and OVCAR3 cells by suppressing cancer cell proliferation, invasion, increasing cisplatin chemosensitivity and inhibiting in vivo tumor growth. Hsa-miR-214-3p was confirmed to directly bind XIST, and inversely downregulated in XIST-upregulated EOC cells. In EOC cells with XIST upregulation, secondary lentiviral transduction successfully upregulated hsa-miR-214-3p expression. Subsequently, hsa-miR-214-3p upregulation functionally reversed the anticancer effects of XIST-upregulation in EOC. Conclusion Upregulation of lncRNA XIST may suppress EOC development, possibly through sponging effect to induce hsa-miR-214-3p downregulation.

Supplemental Digital Content is available in the text, Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver condition worldwide. However, its etiology and fundamental pathophysiology for the disease process are poorly understood. In this study, we thus used bioinformatics to identify candidate genes potentially causative of severe NAFLD. Methods: Gene expression profile data GSE49541 were downloaded from the Gene Expression Omnibus database. Tissues samples from 32 severe and 40 mild NAFLD patients were evaluated to identify differentially expressed genes (DEGs) between the 2 groups, followed by analyses of Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes pathways. Then, a weighted protein–protein interaction (PPI) network was constructed, and subnetworks and candidate genes were screened. Moreover, the GSE48452 data (14 normal liver tissue samples and 18 nonalcoholic steatohepatitis samples) were used to verify the results obtained from the above analyses. Results: A total of 100 upregulated genes and 24 downregulated ones were identified in severe NAFLD. Functional enrichment and pathway analyses showed that these DEGs were mainly associated with cell adhesion, inflammatory response, and chemokine activity. The top 5 subnetworks were selected based on the PPI network. A total of 5 hub genes, including ubiquilin 4 (UBQLN4), amyloid-beta precursor protein (APP), sex hormone–binding globulin (SHBG), cadherin-associated protein beta 1 (CTNNB1) and collagen type I alpha 1 (COL1A1), were considered to be candidate genes for NAFLD. In addition, the verification data confirmed the status of COL1A1, SHBG, and APP as candidate genes. Conclusion: UBQLN4, APP, CTNNB1, SHBG, and COL1A1 might be involved in the development of NAFLD, and are proposed as the potential markers for predicting the development of this condition.

The objective is to find out the causes of Cytopenia post-engraftment (CPE) in hematopoietic stem cell transplantation (HSCT) for patients with β-thalassemia major (β-TM). For this, 61 consecutive β-TM patients underwent 7/8 HLA matched HSCT from January 1, 2009 to June 30, 2015 were retrospectively analyzed. Thirty-eight patients suffered from CPE and the remainder (n=23) associated without CPE. The effect of pre-transplant ferritin (PTF) and recipient and donor age on CPE were analyzed in the two groups. The CPE was defined as white blood cell counts less than 3.0×109/L for four weeks or longer without infection of cytomegalovirus, human parvovirus B19 virus and Epstein-Barr virus. As result, in univariate analysis, PTF level was a high-risk factor for CPE and significant higher in CPE group than no-CPE group (3927.9 ± 1314.9 vs 2291.0 ± 994.4 ng/ml, p=0.000). The result was also proved by multi-factor binary regression analysis (p=0.001). The optimal value of PTF level by R language (R 2.15.2) is 2500ng/ml, which is cutoff value in the two groups. The current study showed high PTF level was a high-risk factor of CPE for β-TM patients underwent 7/8 HLA-matched HSCT. PTF should be reduced below 2500ng/L if the 7/8 matched HSCT must be done.

An UNB pattern of increasing sampling frequency is proposed. M-ary amplitude and M-ary VMSK (MVMSK) CoDec of UNB communication are presented to illustrate the UNB pattern. The UNB pattern can be used for deep space TT&C communication to improve its performance.

The morphology watershed is an effective method of image segmentation. This filter algorithm is as follows: First, basing on elevation information, discrete data point cloud should be turned into a gray image, Then it be pre-filtered with morphological watershed, because of the excessive separate phenomena of watershed morphology, Finally we use the region growing to eliminate or reduce its negative effects. The algorithm does not need to interpolate original data, without iteration, and speed is good. Experimental results show that this filter process can be programmed to obtain the goal of automation and certain accuracy.

A Stream-cipher Slow-frequency-hopping Communication System is proposed, where the stream-cipher sequence is used not only to encrypt the information data, but also to generate frequency-hopping code, thus the stream-cipher slow-frequency-hopping communication system combines the merit of stream-cipher technology and the merit of frequency-hopping technology; a stream-cipher synchronization protocol based on timing is designed; simulation results demonstrated that the principle of the stream-cipher slow-frequency-hopping communication system is correct.

We investigated changes in the susceptibility of Plasmodium falciparum to artesunate in vitro using Rieckmann's microtest in Yunnan Province, China, during the period 1988 to 1999. Longitudinal surveillance studies in 1988, 1992 and 1999 revealed that the IC50s were 6.2, 7.2 and 20.7 nmol/L, respectively and mean concentrations completely inhibiting schizont formation (CIMC) were 37.8, 46.1 and 74.0 nmol/L, respectively. The IC50 and CIMC in 1999 were 3.3 and 2 times greater than in 1988. In cross-sectional tests from 1991 to 1993, the susceptibility of P. falciparum isolates from areas in the western and the southern parts of Yunnan Province were similar, but lower than in the south-eastern and central parts of the province. The results suggest that P. falciparum is generally susceptible to artemisinin derivatives but indicate a reduction in susceptibility during the study period in areas where the drugs have been used for a long time.

Abstract 4514 We used a novel NF-08-TM transplant protocol in 100 consecutive patients with β-thalassemia major (TM), including 66 with allogeneic peripheral blood stem cell transplantation (PBSCT) from unrelated donors (UD) with well-matched human leukocyte antigens (HLAs) and 34 with hematopoietic stem cell transplantation (HSCT) from matched sibling donors (MSD). The median age at transplantation was 6.5 yo (range: 0.6–15 yo), and the ratio of male to female patients was 68:32. The median follow-up time was 24 months (range: 12–39 months). The 3-year overall survival (OS) and TM-free survival (TFS) were 92.4% and 90.9% in the UD-PBSCT group and 88.2% and 82.4% in the MSD-HSCT group. The cumulative incidences of graft rejection (GR) and grade II–IV acute graft-versus-host disease (aGVHD) were 1.5% and 10.6%, respectively, in the UD-PBSCT group and 6.1% and 5.9%, respectively, in the MSD-HSCT group. The cumulative incidence of transplant-related mortality (TRM) was 7.6% in the UD-PBSCT group and 11.8% in the MSD-HSCT group. In conclusion, UD-PBSCTs using the well-tolerated NF-08-TM protocol show similar results to MSD-HSCTs and can be used to treat b thalassemia patients in the absence of MSD. Disclosures: No relevant conflicts of interest to declare.

Abstract 4506 Objective: To investigate the effect of transplantation using bone marrow plus umbilical cord blood from same sibling in children with β-thalassemia major (TM). Methods: Twenty three TM patients undergoing transplantation of bone marrow and umbilical cord blood of same sibling aged from 4.0 to 12 years, 13 boys and 10 girls, were recruited at the Department of Pediatrics, Nanfang Hospital, Southern Medical University from January 2005 to June 2012. The patients were classified into three classes, class¢ñ to class ¢ò 22 cases and class ¢ó 1 case. Donors ranged 1–4 years received 10 Ìg/kg per day of subcutaneous granulocyte colony-stimulating factor (G-CSF) for 5 consecutive days. Bone marrow was harvested on the fifth day. Bone marrow and umbilical cord blood of the same sibling then were transfused into the patient. Results: Recovery of hematopoiesis was gained in all patients 4 weeks following transplantation. Seventeen patients suffered from infection of different degree. Six patients developed mild venous occlusive disease. Four patients developed grade¢ñacute graft-versus-host disease (GVHD), and one developed grade¢ñchronic GVHD. Of twenty three patients, twenty survived, three died of whom, one died of lung infection and heart failure 32 days following transplantation, one died of organ failue on 47days after transplantation, and the other one died of lung fugal infection 22 months after transplantation. Conclusion: Combined transplantation of granulocyte colony-stimulating factor primed bone marrow and umbilical cord blood of same sibling in children with β-thalassemia major is safe and effective with promising results. However, complications should be paid high attention following transplantation. Disclosures: No relevant conflicts of interest to declare.