Search

Additional file 1: Figure S1. The full-length nucleotide sequence for C. tripartitus Toll-like receptor-2 (Ct_TLR-2). Figure S2. Secondary structure prediction of Ct_TLR2 using PSI-PRED (version 4.0). Figure S3. The full-length nucleotide sequence for C. tripartitus CTL (C-type Lectin; Ct_CTL). Figure S4. Secondary structure prediction of Ct_CTL using PSI-PRED (version 4.0). Figure S5. The full-length nucleotide sequence for C. tripartitus Peptidoglycan Recognition Protein SC-2-like (Ct_PGRP_SC-2-like). Figure S6. Secondary structure prediction of Ct_PGRP_SC-2-like using PSI-PRED (version 4.0).

Supplemental material, sj-docx-1-wso-10.1177_17474930231176715 for Effectiveness and safety of secondary prevention of non-vitamin K oral anticoagulants use by drug type in Asian patients by Ki-Woong Nam, Hyung-Min Kwon, Yong-Seok Lee, Sung-Ho Won and Hye-Sung Moon in International Journal of Stroke

Additional file 1. Additional Materials and Methods.

Additional file 2: Table S1. Preprocessing of raw reads obtained from C. tripartitus using Illumina next-generation sequencer. Table S2. Classification of C. tripartitus Candidate genes to the innate immune signaling process. Table S3. Genes of interest related to growth in the dung beetle, C. tripartitus. Table S4. Candidate Sex-Determination and Reproduction related genes from C. tripartitus unigenes.

Additional file 1: Table S1. Items of National Institutes of Health Stroke Scale. Additional file 2: Table S2. Trial of Org 10,172 in Acute Stroke Treatment (TOAST) classification. Additional file 3: Table S3. Biological interaction between initial/follow-up D-dimer levels and initial NIHSS score for early neurological deterioration.

Supplemental material, sj-docx-1-wso-10.1177_17474930231176715 for Effectiveness and safety of secondary prevention of non-vitamin K oral anticoagulants use by drug type in Asian patients by Ki-Woong Nam, Hyung-Min Kwon, Yong-Seok Lee, Sung-Ho Won and Hye-Sung Moon in International Journal of Stroke

Additional file 1. Table S1. Total metabolites in the prefrontal cortex. Table S2. Total metabolites in the hippocampus. Supplementary figure S1. Volcano plot showing significant Pearson���s correlation between metabolites and rodent sociability. Supplementary figure S2. Other linear regression analyses between metabolites and rodent sociability. Supplementary figure S3. ROC analyses between metabolites and rodent genotype.

Additional file 1: Supplementary Figures.

Users of time-resolving experiments at 3rd generation synchrotron light sources deem online bunch length and filling pattern monitoring as an important real-time diagnostic. We developed an on-line monitoring system that can measure bunch lengths and filling pattern using a photodiode, a wideband pre-amplifier, and a sampling digitizer. Two different methods were evaluated to reconstruct the bunch lengths: Gaussian deconvolution method as an approximation scheme and Fourier analysis as a method to restore the original signal by using the power transmission characteristics of the electronic devices in the system, including a bias-tee, a wideband amplifier, cables, and the photodiode. A bunch lengthening experiment has been conducted to compare and verify the results of those two methods of the photodiode and the result of the streak camera images by changing the overall gap voltage of the superconducting RF cavities. In this paper, we elaborate upon the said photodiode-based measurement techniques, and present the experimental results., Proceedings of the 10th International Beam Instrumentation Conference, IBIC2021, Pohang, Rep. of Korea

Additional file 1: Figure S1. Western blot analysis of the Shank2 proteins. The antibody used for the analysis was same with the analysis presented in Fig. 1c. The amount of proteins loading was different and the running time was increased. Figure S2. Multiple sequence alignment and phylogenetic analysis of DNA sequences containing exon 4′ from eight vertebrate species. The novel exon 4′ region was highlighted by red boxes.

Additional file 1: Figure S1. Enhancing proteasome activity does not affect locomotive activity, basal anxiety and fear memory. (A-B) Mice were tested in open field (33 × 33 cm) for 20 min under dim light. (A) Distance moved measured across the 20-min test session in open field test. WT, n = 5 mice; α3ΔN Tg, n = 9 mice. Unpaired t-test, p = 0.2210. (B) Time spent in center (15 cm) zone in open field test. WT, n = 5 mice; α3ΔN Tg, n = 9 mice. Unpaired t-test, p = 0.5440. (C) Time spent in closed and open arm in elevated plus maze test. WT, n = 5 mice; α3ΔN Tg, n = 9 mice. Two-way ANOVA, effect of genotype, F1, 12 = 0.1428, p = 0.7122. (D-E) Mice were trained with three tone (2.8 kHz, 85 dB, 30 s)-shock (0.5 mA, 2 s co-terminated with the tone) pairings in fear conditioning apparatus. (D) Contextual fear memory was tested in the same training apparatus for 5 min at 24 h after the training. WT, n = 11 mice; α3ΔN Tg, n = 11 mice. Unpaired t-test, p = 0.4354. (E) Auditory fear memory was tested in another chamber with the same tone for 3 min at 48 h after the training. WT, n = 11 mice; α3ΔN Tg, n = 11 mice. Unpaired t-test, p = 0.2260. Only male mice were used for open field and elevated plus maze, whereas both male and female mice were used for fear conditioning tests. Data are mean ± SEM.

著者人数: 54名 (所属. 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS): 鈴木, 大介), Number of authors: 54 (Affiliation. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS): Suzuki, Daisuke), Accepted: 2019-03-26, 資料番号: SA1190030000

Figure S1. Representative image of in situ hybridization by using a negative control probe or cross-species probes in human and mouse cortex. (A-B) Human (A) or mouse (B) cortex samples were hybridized with a probe targeting DapB by using a fluorescent or chromogenic hybridization method, respectively. No DapB signal (blue in A, red in B) was detected. Slices were counter-stained with hematoxylin or DAPI. (C-D) Cross-species hybridization. (C) Human cortex sample was hybridized with the mouse Nf1 probe (blue). (D) Mouse cortex sample was hybridized with the human NF1 probe (blue). Slices were counter-stained with hematoxylin. Scale bars, 50â Îźm. (PDF 131 kb)

Objective: To determine associations between cerebral microbleed (CMB) burden with recurrent ischemic stroke (IS) and intracerebral hemorrhage (ICH) risk after IS or TIA. Methods: We identified prospective studies of patients with IS or TIA that investigated CMBs and stroke (ICH and IS) risk during ≥3 months follow-up. Authors provided aggregate summary-level data on stroke outcomes, with CMBs categorized according to burden (single, 2–4, and ≥5 CMBs) and distribution. We calculated absolute event rates and pooled risk ratios (RR) using random-effects meta-analysis. Results: We included 5,068 patients from 15 studies. There were 115/1,284 (9.6%) recurrent IS events in patients with CMBs vs 212/3,781 (5.6%) in patients without CMBs (pooled RR 1.8 for CMBs vs no CMBs; 95% confidence interval [CI] 1.4–2.5). There were 49/1,142 (4.3%) ICH events in those with CMBs vs 17/2,912 (0.58%) in those without CMBs (pooled RR 6.3 for CMBs vs no CMBs; 95% CI 3.5–11.4). Increasing CMB burden increased the risk of IS (pooled RR [95% CI] 1.8 [1.0–3.1], 2.4 [1.3–4.4], and 2.7 [1.5–4.9] for 1 CMB, 2–4 CMBs, and ≥5 CMBs, respectively) and ICH (pooled RR [95% CI] 4.6 [1.9–10.7], 5.6 [2.4–13.3], and 14.1 [6.9–29.0] for 1 CMB, 2–4 CMBs, and ≥5 CMBs, respectively). Conclusions: CMBs are associated with increased stroke risk after IS or TIA. With increasing CMB burden (compared to no CMBs), the risk of ICH increases more steeply than that of IS. However, IS absolute event rates remain higher than ICH absolute event rates in all CMB burden categories.

Recently, LINAC-based radiotherapy equipment are being developed by combining with imaging devices such as CT or MRI, so that it is possible to precisely focus high dose radiation on tumor tissues while minimizing the normal tissue damage. In order to place the diagnostic and treatment devices simultaneously in a confined space, constraints related to interference and volume between the subsystems must be considered. To meet these requirements, the size and weight of the LINAC system need to be reduced, which can be achieved by applying X-band technology. For the purpose of use in IMRT based on image guided radiotherapy, we developed a 9.3 GHz X-band medical LINAC using side-coupled structure. The LINAC is designed to have the accelerating field strength of 16.8 MV/m, and the beam current transmission efficiency of 26 % at the end of accelerating cell when the supplied RF power is at 1.7 MW. Therefore, it can accelerate the electron beam up to 6.2 MeV with having about 90 mA beam current. We plan to carry out the performance test using beam diagnostics system and X-ray measurement system, and the details of design and experimental results of LINAC will be described in this paper., Proceedings of the 29\textsuperscript{th} Linear Accelerator Conf., LINAC2018, Beijing, China

Current advances in radiotherapy are based on the precise imaging techniques, and there is a pressing need for the development of techniques that are capable of visualizing cancer tissues in real time in conjunction with radiotherapy. Indeed, the image-guided radiotherapy systems in which conventional diagnostic tools such as CT and MRI are combined with the linear accelerator (LINAC)-based radiotherapy have been extensively studied. In this work, we mounted 9.3GHz X-band LINAC designed by KERI on the 360 degree-rotatable O-arm system, which allows efficient integration of a diagnostic tool with a radiotherapy equipment. After mounting, the X-ray profile and percentage depth dose were measured by following the quality assurance using the AAPM TG-51,142 protocol. The beam profile symmetry was estimated to be 10².4% with ±3% tolerance. The X-ray dose was also measured by rotating the O-arm to confirm the stability of the mounted X-band LINAC. As a result, the standard deviation of the X-ray dose was shown to be 0.016 while rotating. Therefore, we demonstrate the feasibility of our O-arm X-band LINAC system for use in highly effective radiotherapy with simultaneous CT image guidance., Proceedings of the 29\textsuperscript{th} Linear Accelerator Conf., LINAC2018, Beijing, China

Confirmation of Aβ1–42 oligomerization. Figure S2. Inhibition of PKR restores basal synaptic dysregulation in 5XFAD mice. Figure S3. ICR mice showed the low standard of the freezing behavior in contextual fear conditioning. Figure S4. Neither Aβ1–42 nor PKRi affected basal synaptic transmission and short-term synaptic plasticity. Figure S5. High frequency stimulation (HFS)-induced LTP is normal in 5XFAD mice. (DOCX 709 kb)

Calculation of A2DS2 score. (DOC 25 kb)

Confirmation of Aβ1–42 oligomerization. Figure S2. Inhibition of PKR restores basal synaptic dysregulation in 5XFAD mice. Figure S3. ICR mice showed the low standard of the freezing behavior in contextual fear conditioning. Figure S4. Neither Aβ1–42 nor PKRi affected basal synaptic transmission and short-term synaptic plasticity. Figure S5. High frequency stimulation (HFS)-induced LTP is normal in 5XFAD mice. (DOCX 709 kb)

Diagnostic criteria for stroke associated pneumonia based on the CDC criteria. (DOCX 25 kb)

We firstly show the prototype of an ohmic contact Single-Pole Double-Throw Radio Frequency Micro-Electro-Mechanical Systems (SPDT RF MEMS) switch operating at 50–100GHz. The fabricated ohmic contact SPDT RF MEMS silicon switch moves both laterally and vertically, to improve the isolation at high frequencies by initially misaligning the contact part of the switch over a Coplanar Waveguide (CPW) transmission line. The lateral and vertical movement of the switch is operated by using comb and parallel plate actuators, respectively. The proposed switch was fabricated using Silicon-On-Glass (SiOG) bonding process. The insertion loss of the fabricated switch is measured according to the different operation states of the switch, in the range from 50 to 100GHz. The fabricated length of the transmission line is 4.6mm and the measured insertion loss and isolation are 9.13dB and 24.37dB at 70GHz, respectively.

International audience; Ticagrelor may be a more effective antiplatelet therapy than aspirin for the prevention of recurrent stroke and cardiovascular events in patients with acute cerebral ischemia.; We conducted an international double-blind, controlled trial in 674 centers in 33 countries, in which 13,199 patients with a nonsevere ischemic stroke or high-risk transient ischemic attack who had not received intravenous or intraarterial thrombolysis and were not considered to have had a cardioembolic stroke were randomly assigned within 24 hours after symptom onset, in a 1:1 ratio, to receive either ticagrelor (180 mg loading dose on day 1 followed by 90 mg twice daily for days 2 through 90) or aspirin (300 mg on day 1 followed by 100 mg daily for days 2 through 90). The primary end point was the time to the occurrence of stroke, myocardial infarction, or death within 90 days.; During the 90 days of treatment, a primary end-point event occurred in 442 of the 6589 patients (6.7%) treated with ticagrelor, versus 497 of the 6610 patients (7.5%) treated with aspirin (hazard ratio, 0.89; 95% confidence interval [CI], 0.78 to 1.01; P=0.07). Ischemic stroke occurred in 385 patients (5.8%) treated with ticagrelor and in 441 patients (6.7%) treated with aspirin (hazard ratio, 0.87; 95% CI, 0.76 to 1.00). Major bleeding occurred in 0.5% of patients treated with ticagrelor and in 0.6% of patients treated with aspirin, intracranial hemorrhage in 0.2% and 0.3%, respectively, and fatal bleeding in 0.1% and 0.1%.; In our trial involving patients with acute ischemic stroke or transient ischemic attack, ticagrelor was not found to be superior to aspirin in reducing the rate of stroke, myocardial infarction, or death at 90 days. (Funded by AstraZeneca; ClinicalTrials.gov number, NCT01994720.).

A list of identified variants that were not detected in unaffected members but detected in affected members (PDF 35 kb)

CD38−/− mouse shows normal locomotion and basal anxiety. (A) CD38+/+ (black) and CD38−/− (blue) mice spent comparable time at the center of the open field (CD38+/+, n = 7; CD38−/−, n = 10; unpaired t-test, P = 0.272). (B) CD38+/+ (black) and CD38−/− (blue) mice showed similar level of anxiety assessed in the elevated plus maze test (CD38+/+, n = 12; CD38−/−, n = 9; two-way ANOVA, interaction F (1, 2) = 0.119, p = 0.888). (C) Deletion of CD38 gene did not cause any decrease in total distance moved in the open field test (CD38+/+, n = 7; CD38−/−, n = 10; unpaired t-test, p = 0.366). All graphs were plotted mean ± SEM. Figure S2. CD38−/− mouse shows normal TBS-induced E-LTP. TBS-induced E-LTP at SC-CA1 synapses showed no difference in WT and CD38−/−mice (CD38+/+, n = 7, 157.4 ± 4.4 %; CD38−/−, n = 6, 171.5 ± 6.1 %; unpaired t-test, p = 0.0808, n.s.). (PDF 124 kb)

Figure S1. A representative Western blot (left) and quantification (right) of ApCPEB4 in Aplysia pleural ganglia extracts prepared from animals exposed to 5-HT in vivo for 2Â h. Total extracts were prepared at indicated times and 20Â Îźg of proteins were blotted with anti-ApCPEB4 antibodies (left, top panel). The same extracts were also stained with Coomassie blue as loading controls (left, bottom panel). (PDF 49 kb)

This paper presents a single-pole doublethrow (SPDT) RF MEMS ohmic contact switch operating from 50 to 70 GHz. The switch is fabricated using the silicon on glass (SiOG) process. Conventional switches are actuated in one direction (vertical direction), whereas the proposed switch is actuated in two directions (vertical and horizontal directions). By operating two actuators, the switch has high isolation. The RF characteristics are measured from 50 to 70 GHz. The measured insertion loss is more than 20dB before actuation. After the on-state is achieved by the two actuators, the insertion loss is measured and found to be about 10 dB.

The RF system for the Pohang Light Source (PLS) storage ring was greatly upgraded for PLS-II project of 400mA, 3.0GeV from 200mA, 2.5GeV. Three superconducting RF cavities with each 300kW maximum klystron amplifier were commissioned with electron beam in way of one by one during the last 3 years for beam current of 400mA to until March 2014. The RF system is designed to provide stable beam through precise RF phase and amplitude requirements to be less than 0.3% in amplitude and 0.3° in phase deviations. This paper describes the RF system configuration, design details and test results., Proceedings of the 6th Int. Particle Accelerator Conf., IPAC2015, Richmond, VA, USA

The design of RF amplifier of 100 kW (CW) at 165MHz is studied for a Radio Frequency Quadruple (RFQ). The RFQ as a linear accelerator is used for acceleration of low energy beam to 500 keV/u at KBSI [1]. An RF amplifier is composed of a drive, an intermediate, and a final amplifier stage with power supplies. The intermediate amplifier (IPA) of 5 kW is designed with solid state amplifier modules, and the final amplifier is designed with a tetrode tube. The high voltage power supply for the tetrode provides the fine regulation of 15 kV at 10 A. The RF amplifier is operated by program logic controller (PLC) with interlocks, and a low level RF control for RFQ accelerator. This paper describes the present design study on the 100 kW RF amplifier., Proceedings of the 6th Int. Particle Accelerator Conf., IPAC2015, Richmond, VA, USA

Electron linear accelerators are used as x-ray generators for diagnosing the human body. In this paper conceptual design of electron beam for compact electron gun was calculated by using EGN2w and CST-Particle Studio codes. The structure of the electron gun was used for Pierce and diode type and the specification of electron beam was selected as 500 cGy/min. Specifications of designed electron gun were focused on current, beam size and normalized emittance. Optimized beam current, diameter and normalized emittance are 226.88 mA, 0.689 mm (Full width) and 1.03π mm• mrad, respectively by using two simulation codes. Accuracy of simulation was verified by comparison of emitted beam current which has error of 0.74%., Proceedings of the 5th Int. Particle Accelerator Conf., IPAC2014, Dresden, Germany

A mineral phosphate solubilizing bacterium, Burkholderia cepacia DA23 has been isolated from cultivated soils. Phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. When 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to the pH drop by the strain. Analysis of the culture medium by high pressure liquid chromatography identified gluconic acid as the main organic acid released by Burkholderia cepacia DA23. Gluconic acid production was apparently the result of the glucose dehydrogenase activity and glucose dehydrogenase was affected by phosphate regulation. Uma bactéria capaz de solubilizar fosfato mineral, Burkholderia cepacea DA23, foi isolada de solo cultivado. A capacidade dessa bactéria solubilizar o fosfato de três tipos de fosfato insolúvel foi quantificada. Quando foi utilizada glicose a 3% como fonte de carbono, a bactéria apresentou uma intensa atividade solubilizante de fosfato, sendo a solubilização diretamente relacionada com a queda de pH causada pela bactéria. A análise do meio de cultura por cromatografia líquida de alta pressão indicou o ácido glicônico como principal ácido produzido por Burkholderia cepacea DA23. Aparentemente, a produção de ácido glicônico foi causada pela atividade da glicose desidrogenase. A enzima foi afetada pela regulação do fosfato.

CRM is an approach to a basic principle, which marketing fees for alluring new customers takes triple the amount of maintaining existing customers. Of course options like database marketing and e-mail marketing can be used to maintain existing customers. As shown in this paper the implemented eCRM system which is the base of multi-agent and is classified not only as operational CRM but also as collaborative CRM and analytical CRM. This was implemented through basis of specialized components and it supports the concept of ASP service. Through the development of a module, which helps to use existing data environment more effectively, it supports and creates an environment that users can use easily.