1. miR-421 is a diagnostic and prognostic marker in patients with osteosarcoma
- Author
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Jiang Mengzhen, Mingyu Wu, Xuming Yang, Bing Wang, Zhou Shuguang, Liming Qin, Zhou Yucheng, and Jun Hu
- Subjects
musculoskeletal diseases ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Normal tissue ,Bone Neoplasms ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,Cell Line, Tumor ,Healthy volunteers ,microRNA ,Overall survival ,Biomarkers, Tumor ,Medicine ,Diagnostic biomarker ,Humans ,In patient ,Neoplasm Invasiveness ,neoplasms ,Cell Proliferation ,Osteosarcoma ,business.industry ,General Medicine ,medicine.disease ,Prognosis ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,Case-Control Studies ,Cancer research ,business - Abstract
MicroRNAs (miRNAs) are a new class of prognostic and diagnostic biomarkers for many cancers. Recent studies have shown that miRNAs are highly stable in plasma/serum. The aim of this study was to investigate the role of miR-421 in osteosarcoma. We found that the serum expression levels of miR-421 were significantly higher in osteosarcoma patients than those in healthy volunteers. Moreover, miR-421 expression was significantly higher in osteosarcoma tissues compared with that in the adjacent normal tissues. Meanwhile, the expression of miR-421 was upregulated in 90 % (36/40) osteosarcoma tissues compared to non-tumor tissues. More importantly, the expression levels of miR-421 in osteosarcoma tissues were correlated with those in patients' serum. In addition, patients with high miR-421 expression had shorter overall survival (OS) than those with low expressions. We also found that overexpression of miR-421 promoted osteosarcoma cell line MG-63 proliferation, migration, and invasion. In conclusion, miR-421 expression levels were upregulated in osteosarcoma tissue and serum and it may be a useful marker for diagnosis of osteosarcoma.
- Published
- 2015