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International audience; Emerging rodent-borne hantaviruses cause severe diseases in humans with no approved vaccines or therapeutics. We recently isolated a monoclonal broadly neutralizing antibody (nAb) from a Puumala virus-experienced human donor. Here, we report its structure bound to its target, the Gn/Gc glycoprotein heterodimer comprising the viral fusion complex. The structure explains the broad activity of the nAb: It recognizes conserved Gc fusion loop sequences and the main chain of variable Gn sequences, thereby straddling the Gn/Gc heterodimer and locking it in its prefusion conformation. We show that the nAb's accelerated dissociation from the divergent Andes virus Gn/Gc at endosomal acidic pH limits its potency against this highly lethal virus and correct this liability by engineering an optimized variant that sets a benchmark as a candidate pan-hantavirus therapeutic.

Broadly protective vaccines against known and preemergent human coronaviruses (HCoVs) are urgently needed. To gain a deeper understanding of cross-neutralizing antibody responses, we mined the memory B cell repertoire of a convalescent severe acute respiratory syndrome (SARS) donor and identified 200 SARS coronavirus 2 (SARS-CoV-2) binding antibodies that target multiple conserved sites on the spike (S) protein. A large proportion of the non-neutralizing antibodies display high levels of somatic hypermutation and cross-react with circulating HCoVs, suggesting recall of preexisting memory B cells elicited by prior HCoV infections. Several antibodies potently cross-neutralize SARS-CoV, SARS-CoV-2, and the bat SARS-like virus WIV1 by blocking receptor attachment and inducing S1 shedding. These antibodies represent promising candidates for therapeutic intervention and reveal a target for the rational design of pan-sarbecovirus vaccines.

The main objective of this study is to find the authorship patterns and degree of collaboration of Central University of Gujarat with a total number of 301 publications during the period 2010 to 2017 derived from Scopus database. The research method of this study was scientometric analysis method. The main research area of Central University of Gujarat was Chemistry. The most preferred journal for publication was Journal of Molecular Liquids and the most prolific author was Prof. Man Singh. The present study revealed several numbers of scientometric indicators like citation pattern, collaborative institutions, most prolific authors etc.

It is accomplished study regarding the subject of bibliometric under which the citation trends have been evaluated for scholarly publication in the journal 'Library Trends' during the period 2012 to 2016.

At present era, browsers widely using the HTML tags for better readability of web pages for humans. The new specifications of HTML, HTML5 provides a very good mechanism to define a structure in web pages. The mechanism popularly known as microdata is easier to implement than the other formats such as microformats and RDFa. These structures are machine-readable which are providing the software programs to search for specific types of information. Recently, some of the popular search engines such as Google, Yahoo and Bing joined hands to support microdata. But now JSON-LD is a growing description at present. Schema.org, a collection of terms that webmasters can use to markup their pages to improve the display of search results. This paper covers a way for content description metadata through some examples like Microdata, RDFa, Microformats, JSON-LD, Rich Snippets etc.

It is accomplished study regarding the subject of bibliometric under which the citation trends have been evaluated of scholarly publication in the journal 'Library Trends' during the period 2012 to 2016.

It is accomplished study regarding the subject of bibliometric under which the citation trends have been evaluated of scholarly publication in the journal 'Library Trends' during the period 2012 to 2016.

The traditional MARC bibliographic format extremely used for library bibliographic data from becoming network information resources. The library must solve how to attracts the users from network application services back to the library system. The present paper discusses SchemaBibEx, which is the bibliographic resource description extension of Schema.org, as the powerful tool for the library to solve this problem. By analysing the linked data model, vocabularies, tools, and services, the paper explains its originality, openness, global unity, and economically. It also discusses its complementary relationship with BIBFRAME which is the next generation of the bibliographic frame format. SchemaBibEx is not the replacement of MARC but will become a two-way communication bridge between library bibliographic data and information user network.

At present era, browsers widely using the HTML tags for better readability of web pages for humans. The new specifications of HTML, HTML5 provides a very good mechanism to define a structure in web pages. The mechanism popularly known as microdata is easier to implement than the other formats such as microformats and RDFa. These structures are machine-readable which are providing the software programs to search for specific types of information. Recently, some of the popular search engines such as Google, Yahoo and Bing joined hands to support microdata. But now JSON-LD is a growing description at present. Schema.org, a collection of terms that webmasters can use to markup their pages to improve the display of search results. This paper covers a way for content description metadata through some examples like Microdata, RDFa, Microformats, JSON-LD, Rich Snippets etc.

This presentation describes academic research metrics.

The interferon-stimulated gene viperin has been shown to have antiviral activity against hepatitis C virus (HCV) in the context of the HCV replicon, although the molecular mechanisms responsible are not well understood. Here we demonstrate that viperin plays an integral part in the ability of interferon to limit replication of cell culture derived HCV (JFH-1) that accurately reflects the complete viral life cycle. Using confocal microscopy and Fluorescence Resonance Energy Transfer (FRET) analysis we demonstrate that viperin localizes and interacts with HCV NS5A at the lipid droplet interface. In addition viperin also associates with NS5A and the pro-viral cellular factor, VAP-A at the HCV replication complex. The ability of viperin to limit HCV replication was dependent on residues within the C-terminus as well as an N-terminal amphipathic helix. Removal of the amphipathic helix redirected viperin from the cytosolic face of the ER and the lipid droplet to a homogenous cytoplasmic distribution, coinciding with a loss of antiviral effect. C-terminal viperin mutants still localized to the lipid droplet interface and replication complexes but did not interact with NS5A proteins as determined by FRET analysis. In conclusion we propose that viperin interacts with NS5A and the host factor VAP-A to limit HCV replication at the replication complex. This highlights the complexity of host control of viral replication by interferon stimulated gene expression.