Search

Your search keyword '"Inga Tijunelyte"' showing total 13 results
Start Over Author "Inga Tijunelyte" Database OpenAIRE
13 results on '"Inga Tijunelyte"'

1. Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography

Author

Inga Tijunelyte, Jeffrey Teillet, Paul Bruand, Rémi Courson, Aurélie Lecestre, Pierre Joseph, Aurélien Bancaud, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), ANR-16-CE18-0028,MicroLAS,µ-Laboratoire d'Analyse et de Séparation des chromosomes : développement d'un outil pour le typage rapide des bactéries, levures et cellules de mammifères(2016), and ANR-16-ASTR-0020,BIOPULSE,Dispositif de détection biologique ultrasensible de pathogènes prélevés par air pulsé.(2016)

Subjects
Fluid Flow and Transfer Processes, [SPI]Engineering Sciences [physics], Colloid and Surface Chemistry, Biomedical Engineering, General Materials Science, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Condensed Matter Physics, [SPI.MAT]Engineering Sciences [physics]/Materials
Abstract

International audience; Speeding up and enhancing the performances of nucleic acid biosensing technologies have remained drivers for innovation. Here, we optimize a fluorimetry-based technology for DNA detection based on the concentration of linear targets paired with probes. The concentration module consists of a microfluidic channel with the shape of a funnel in which we monitor a viscoelastic flow and a counter-electrophoretic force. We report that the technology performs better with a target longer than 100 nucleotides (nt) and a probe shorter than 30 nt. We also prove that the control of the funnel geometry in 2.5D using grayscale lithography enhances sensitivity by 100-fold in comparison to chips obtained by conventional photolithography. With these optimized settings, we demonstrate a limit of detection of 4 fM in 30 s and a detection range of more than five decades. This technology hence provides an excellent balance between sensitivity and time to result.

Published
2022
Full Text
View/download PDF

2. Sensing Polymer/Paracetamol Interaction with an Independent Component Analysis-Based SERS-MIP Nanosensor

Author

N. Decorbie, Inga Tijunelyte, Nordin Félidj, Jeanne Solard, Aazdine Lamouri, Caroline Gauchotte-Lindsay, Nathalie Lidgi-Guigui, Sarra Gam-Derouich, Emmanuel Rinnert, Claire Mangeney, and Philippe Decorse

Subjects
Materials science, Biophysics, Nanotechnology, Independent component analysis, 02 engineering and technology, Emerging pollutant, 01 natural sciences, Biochemistry, 010309 optics, symbols.namesake, Nanosensor, 0103 physical sciences, Molecule, Statistical analysis, Sensor, chemistry.chemical_classification, SERS, Molecularly imprinted polymer, Polymer, 021001 nanoscience & nanotechnology, Highly selective, Paracetamol, chemistry, Molecularly imprinted polymers, symbols, 0210 nano-technology, Raman spectroscopy, Biotechnology
Abstract

In this article, we propose a new strategy to build a sensor for easy handling and rapid analysis on-site. Our sensor is based on the combination of surface-enhanced Raman spectroscopy (SERS) and molecularly imprinted polymers (MIPs). SERS provides a strong sensitivity for the detection of trace molecules while MIPs offer a highly selective and specific recognition platform. The research presented here focuses on the detection of the interaction between a robust ultra-thin layer of MIPs and of paracetamol, the targeted molecule. This drug is an environmental emerging pollutant, i.e., a molecule whose presence and significance have not yet been elucidated, which gives rise to health and environmental concerns. The results are a combined analysis of the SERS spectra and a multivariate analysis. The former provides a clear demonstration of the evolution of the MIP-nanostructure interaction when the concentration of paracetamol increases. The statistical analysis produces the proof of the selectivity of the sensor.

Published
2020
Full Text
View/download PDF

3. micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation

Author

Pierre Cordelier, Bayan Chami, Thierry Leichle, Christophe Dez, Rémi Malbec, Jean Cacheux, Inga Tijunelyte, Aurélien Bancaud, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Équipe Microsystèmes électromécaniques (LAAS-MEMS), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches en Cancérologie de Toulouse (CRCT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE18-0028,MicroLAS,µ-Laboratoire d'Analyse et de Séparation des chromosomes : développement d'un outil pour le typage rapide des bactéries, levures et cellules de mammifères(2016), ANR-16-ASTR-0020,BIOPULSE,Dispositif de détection biologique ultrasensible de pathogènes prélevés par air pulsé.(2016), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Centre National de la Recherche Scientifique (CNRS)-Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Georgia Tech Lorraine [Metz], Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Ecole Supérieure d'Electricité - SUPELEC (FRANCE)-Georgia Institute of Technology [Atlanta]-CentraleSupélec-Ecole Nationale Supérieure des Arts et Metiers Metz-Centre National de la Recherche Scientifique (CNRS), School of Electrical and Computer Engineering - Georgia Insitute of Technology (ECE GeorgiaTech), and Georgia Institute of Technology [Atlanta]

Subjects
Materials science, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Microfluidics, Biomedical Engineering, Biophysics, Biosensing Techniques, 02 engineering and technology, Electrohydrodynamic migration, 01 natural sciences, Fluorescence spectroscopy, Fine needle biopsy, [SPI]Engineering Sciences [physics], Microfluidic channel, Electrochemistry, RNA/DNA concentration, Humans, fine needle biopsy, Detection limit, Reverse Transcriptase Polymerase Chain Reaction, 010401 analytical chemistry, General Medicine, 021001 nanoscience & nanotechnology, 0104 chemical sciences, MicroRNAs, RNA, Electrohydrodynamics, miR-21, 0210 nano-technology, Biotechnology, Biomedical engineering
Abstract

International audience; We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This concentration module consists of a microfluidic channel with the shape of a funnel operated with electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than one minute for miR-21 alone, and then demonstrate that miR-21 levels measured in fine needle biopsy samples from patients with pancreatic cancer correlate with the reference technique of reverse-transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising clinical performances for the follow-up of patients with cancer.

Published
2021
Full Text
View/download PDF

4. Characterization and minimization of band broadening in DNA electrohydrodynamic migration for enhanced size separation

Author

Aurélien Bancaud, Bayan Chami, Jeffrey Teillet, Inga Tijunelyte, Quentin Martinez, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), ANR-16-CE18-0028,MicroLAS,µ-Laboratoire d'Analyse et de Séparation des chromosomes : développement d'un outil pour le typage rapide des bactéries, levures et cellules de mammifères(2016), ANR-16-ASTR-0020,BIOPULSE,Dispositif de détection biologique ultrasensible de pathogènes prélevés par air pulsé.(2016), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), and Université Fédérale Toulouse Midi-Pyrénées

Subjects
Electrophoresis, Materials science, Field (physics), [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Microfluidics, Taylor dispersion, 02 engineering and technology, Parameter space, 01 natural sciences, Viscoelasticity, Diffusion, Electric field, Diffusion (business), Viscosity, 010401 analytical chemistry, Povidone, General Chemistry, Mechanics, DNA, Models, Theoretical, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Elasticity, 0104 chemical sciences, Hydrodynamics, Electrohydrodynamics, 0210 nano-technology
Abstract

International audience; The combination of hydrodynamic actuation with an opposing electrophoretic force in viscoelastic liquids enables the separation, concentration, and purification of DNA. Obtaining good analytical performances despite the use of hydrodynamic flow fields, which dramatically enhance band broadening due to Taylor dispersion, constitutes a paradox that remains to be clarified. Here, we study the mechanism of band broadening in electrohydrodynamic migration with an automated microfluidic platform that allows us to track the migration of a 600 bp band in the pressure-electric field parameter space. We demonstrate that diffusion in the electrohydrodynamic regime is controlled predominantly by the electric field and marginally by the hydrodynamic flow velocity. We explain this response with an analytical model of diffusion based on Taylor dispersion arguments. Furthermore, we demonstrate that the electric field can be modulated over time to monitor and minimize the breadth of a DNA band, and suggest guidelines to enhance the resolution of DNA separation experiments. Altogether, our report is a leap towards to the development of high-performance analytical technologies based on electrohydrodynamic actuation.

Published
2020
Full Text
View/download PDF

5. Tunable Multilayers of Self-Organized Silica Nanospheres by Spin Coating

Author

Alexis Fischer, J. Solard, Marc Lamy de la Chapelle, Nathalie Lidgi-Guigui, Sofiane Kasmi, Inga Tijunelyte, Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC), Laboratoire de Physique des Lasers (LPL), and Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Article Subject, 02 engineering and technology, engineering.material, 010402 general chemistry, 01 natural sciences, law.invention, Coating, law, lcsh:Technology (General), General Materials Science, Lithography, [PHYS]Physics [physics], Spin coating, business.industry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Volume fraction, engineering, lcsh:T1-995, Optoelectronics, Nanosphere lithography, Photolithography, 0210 nano-technology, business, Layer (electronics)
Abstract

International audience; The coating of fused silica by an organized layer of silica nanospheres (NS) is an important issue for the design of optical and topographic properties especially for lithography techniques such as nanosphere lithography (NSL) or nanosphere photolithography (NSPL). Here, the spin coating of NS dispersed in N, N-dimethylformamide (DMF) is studied. The role of the NS diameter, the spin-coating acceleration, and the volume fraction are the parameters to take into account for the formation and organization of NS in single or double closely packed layers. We propose an explanation for this behavior based on the transition between sedimentation and a viscous regime on the basis of the silica NS organization.

Published
2018
Full Text
View/download PDF

7. Multi-functionalization of lithographically designed gold nanodisks by plasmon-mediated reduction of aryl diazonium salts

Author

Nordin Félidj, Georges Lévi, Issam Kherbouche, Jean Aubard, M. Lamy de la Chapelle, Mai Nguyen, Alexandre Chevillot-Biraud, Aazdine Lamouri, Nathalie Lidgi-Guigui, Sarra Gam-Derouich, Claire Mangeney, Inga Tijunelyte, Institut des Nanosciences de Paris (INSP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Leprince-Ringuet (LLR), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Centre National de la Recherche Scientifique (CNRS), Institut des Matériaux Jean Rouxel (IMN), Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Evolution des régulations endocriniennes (ERE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Sorbonne Paris Nord, Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Graduate University of Science and Technology [Hanoi] (GUST), Vietnam Academy of Science and Technology (VAST), and Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601)

Subjects
[PHYS]Physics [physics], Materials science, Plasmon excitation, Aryl, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Photochemistry, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, chemistry, Surface modification, General Materials Science, 0210 nano-technology, Plasmon, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Plasmon-driven surface functionalization of nanoparticles is receiving growing attention as it allows generating locally tailored chemical reactivity on the nanoparticle surface. The extension to surface multi-functionalization still represents a major breakthrough in chemistry. We address this issue by triggering regiospecific surface double-functionalization under plasmon excitation, using diazonium salts as surface functionalization agents.

Published
2018
Full Text
View/download PDF

8. Investigation of aromatic hydrocarbon inclusion into cyclodextrins by Raman spectroscopy and thermal analysis

Author

Marc Lamy de la Chapelle, Erwann Guénin, Nathalie Lidgi-Guigui, Irena Milosevic, Carole Barbey, Inga Tijunelyte, Emmanuel Rinnert, Nathalie Dupont, Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Centre National de la Recherche Scientifique (CNRS), Powder Technology Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire (BIOMOCETI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Transformation Intégrée de la Matière Renouvelable (TIMR), Université de Technologie de Compiègne (UTC), Université Sorbonne Paris Cité (USPC)-Institut Galilée-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC), and Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], Health, Toxicology and Mutagenesis, Context (language use), Environmental pollution, 02 engineering and technology, Spectrum Analysis, Raman, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Computational chemistry, Cyclodextrin, [CHIM]Chemical Sciences, Environmental Chemistry, Organic chemistry, Polycyclic Aromatic Hydrocarbons, ComputingMilieux_MISCELLANEOUS, Naphthalene, chemistry.chemical_classification, Fluoranthene, Cyclodextrins, Host-guest complex, Cavitand, Thermogravimetric analysis, PAH, General Medicine, 021001 nanoscience & nanotechnology, Pollution, Toluene, 0104 chemical sciences, chemistry, 13. Climate action, Thermogravimetry, Raman spectroscopy, 0210 nano-technology, Aromatic hydrocarbon, Water Pollutants, Chemical, Environmental Monitoring, Polyaromatic hydrocarbons
Abstract

Among various cavitand molecules, cyclodextrins are extensively studied due to their ability to form host-guest complexes with small hydrophobic molecules. Aiming to explore cyclodextrin implementation on the scopes related to the environmental pollution monitoring or remediation, extensive studies for understanding the cyclodextrin-based host-guest complex formation with selected targeted substances are conducted. In this context, two polycyclic aromatic hydrocarbons, naphthalene and fluoranthene as well as toluene as a member of volatile organic compounds, were studied regarding their ability to encapsulate into cyclodextrin cavities. Synthesised complexes were examined by thermogravimetric analysis combined with Raman spectroscopy. The obtained results demonstrated that the size between targeted molecules and the cyclodextrin cavities strongly correlates with its ability to engage in complexation. Thus, this latter parameter plays an important role in the inclusion complex formation as well as in the strength of the interaction between the molecules.

Published
2015
Full Text
View/download PDF

9. Diazonium Salt-Based Surface-Enhanced Raman Spectroscopy Nanosensor: Detection and Quantitation of Aromatic Hydrocarbons in Water Samples

Author

Chapelle, Inga Tijunelyte, Stéphanie Betelu, Jonathan Moreau, Ioannis Ignatiadis, Catherine Berho, Nathalie Lidgi-Guigui, Erwann Guénin, Catalina David, Sébastien Vergnole, Emmanuel Rinnert, and Marc Lamy de la

Subjects
polynuclear aromatic hydrocarbon (PAH), surface-enhanced Raman spectroscopy (SERS), nanosensor, diazonium salt, surface functionalization, detection
Abstract

Here, we present a surface-enhanced Raman spectroscopy (SERS) nanosensor for environmental pollutants detection. This study was conducted on three polycyclic aromatic hydrocarbons (PAHs): benzo[a]pyrene (BaP), fluoranthene (FL), and naphthalene (NAP). SERS substrates were chemically functionalized using 4-dodecyl benzenediazonium-tetrafluoroborate and SERS analyses were conducted to detect the pollutants alone and in mixtures. Compounds were first measured in water-methanol (9:1 volume ratio) samples. Investigation on solutions containing concentrations ranging from 10−6 g L−1 to 10−3 g L−1 provided data to plot calibration curves and to determine the performance of the sensor. The calculated limit of detection (LOD) was 0.026 mg L−1 (10−7 mol L−1) for BaP, 0.064 mg L−1 (3.2 × 10−7 mol L−1) for FL, and 3.94 mg L−1 (3.1 × 10−5 mol L−1) for NAP, respectively. The correlation between the calculated LOD values and the octanol-water partition coefficient (Kow) of the investigated PAHs suggests that the developed nanosensor is particularly suitable for detecting highly non-polar PAH compounds. Measurements conducted on a mixture of the three analytes (i) demonstrated the ability of the developed technology to detect and identify the three analytes in the mixture; (ii) provided the exact quantitation of pollutants in a mixture. Moreover, we optimized the surface regeneration step for the nanosensor.

Published
2017
Full Text
View/download PDF

10. Diazonium Salt-Based Surface-Enhanced Raman Spectroscopy Nanosensor: Detection and Quantitation of Aromatic Hydrocarbons in Water Samples

Author

Inga, Tijunelyte, Stéphanie, Betelu, Jonathan, Moreau, Ioannis, Ignatiadis, Catherine, Berho, Nathalie, Lidgi-Guigui, Erwann, Guénin, Catalina, David, Sébastien, Vergnole, Emmanuel, Rinnert, and Marc, Lamy de la Chapelle

Subjects
surface-enhanced Raman spectroscopy (SERS), diazonium salt, detection, nanosensor, Article, polynuclear aromatic hydrocarbon (PAH), surface functionalization
Abstract

Here, we present a surface-enhanced Raman spectroscopy (SERS) nanosensor for environmental pollutants detection. This study was conducted on three polycyclic aromatic hydrocarbons (PAHs): benzo[a]pyrene (BaP), fluoranthene (FL), and naphthalene (NAP). SERS substrates were chemically functionalized using 4-dodecyl benzenediazonium-tetrafluoroborate and SERS analyses were conducted to detect the pollutants alone and in mixtures. Compounds were first measured in water-methanol (9:1 volume ratio) samples. Investigation on solutions containing concentrations ranging from 10−6 g L−1 to 10−3 g L−1 provided data to plot calibration curves and to determine the performance of the sensor. The calculated limit of detection (LOD) was 0.026 mg L−1 (10−7 mol L−1) for BaP, 0.064 mg L−1 (3.2 × 10−7 mol L−1) for FL, and 3.94 mg L−1 (3.1 × 10−5 mol L−1) for NAP, respectively. The correlation between the calculated LOD values and the octanol-water partition coefficient (Kow) of the investigated PAHs suggests that the developed nanosensor is particularly suitable for detecting highly non-polar PAH compounds. Measurements conducted on a mixture of the three analytes (i) demonstrated the ability of the developed technology to detect and identify the three analytes in the mixture; (ii) provided the exact quantitation of pollutants in a mixture. Moreover, we optimized the surface regeneration step for the nanosensor.

Published
2017

11. Evidence of the Grafting Mechanisms of Diazonium Salts on Gold Nanostructures

Author

Stéphane Gaboreau, Joyce Ibrahim, Emmanuel Rinnert, Nathalie Lidgi-Guigui, Inga Tijunelyte, Timothée Toury, Erwann Guénin, Ioannis Ignatiadis, Nordin Félidj, Catherine Berho, Leïla Boubekeur-Lecaque, Marc Lamy de la Chapelle, Stéphanie Betelu, Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Nanotechnologie et d'Instrumentation Optique (LNIO), Institut Charles Delaunay (ICD), Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS), Agence Nationale pour la Gestion des Déchets Radioactifs (ANDRA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Sorbonne Paris Cité (USPC)-Institut Galilée-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)

Subjects
Nanostructure, Materials science, Aromaticity, 02 engineering and technology, engineering.material, 010402 general chemistry, 021001 nanoscience & nanotechnology, Ring (chemistry), Grafting, 01 natural sciences, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, [SPI.MAT]Engineering Sciences [physics]/Materials, symbols.namesake, General Energy, X-ray photoelectron spectroscopy, Coating, Polymer chemistry, symbols, engineering, Organic chemistry, Physical and Theoretical Chemistry, 0210 nano-technology, Raman spectroscopy
Abstract

International audience; Gold nanostructures (GNS) were chemically functionalized using four different diazonium salts: benzene-diazonium-tetrafluoroborate (DS), 4-decylbenzene-diazonium-tetrafluoroborate (DS-C10H21), 4-carboxybenzene-diazonium-tetrafluoroborate (DS-COOH), and 4-(aminoethyl)-benzene-diazonium-tetrafluoroborate (DS-(CH2)2NH2). Effective chemical grafting on GNS was shown by surface-enhanced Raman spectroscopy (SERS); aromatic ring deformations in the range of 1570–1591 cm–1 are of particular interest. The very strong band observed around 1075 cm–1, related to CH in-plane bending for mono- and para-substituted benzenes (coupled with ring-N stretching mode), provided further irrefutable evidence of the grafting. SERS enhancement of these two bands ascertains the perpendicular orientation of the aromatic rings on the GNS. X-ray photoelectron spectroscopy (XPS) analyses of chemically grafted flat gold surfaces suggest azophenyl radical pathways when using DS, DS-(CH2)2-NH2, or DS-C10H21. It was shown that coating at the interface is the result of a Au–N covalent bond; growth of the layers is via N═N. These XPS results agree with those provided by SERS without excluding the aryl radical pathways. For DS-COOH, the results provided by SERS, XPS, and density functional theory calculations show (i) effective chemical grafting of the GNS via a covalent bond between gold and carboxylate forms and (ii) growth via multilayers in the meta position between aromatic rings through either N═N or C–C bonds.

Published
2016
Full Text
View/download PDF

12. Soft UV nanoimprint lithography-designed highly sensitive substrates for SERS detection

Author

Bernard Bartenlian, Grégory Barbillon, Marc Lamy de la Chapelle, Philippe Gogol, Maximilien Cottat, Nathalie Lidgi-Guigui, Abdelhanin Aassime, Inga Tijunelyte, Jean-Michel Lourtioz, and Frédéric Hamouda

Subjects
Materials science, Biotin, Nanochemistry, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Nanoimprint lithography, law.invention, symbols.namesake, Materials Science(all), law, Soft UV-NIL, General Materials Science, Nanoimprint, Nano Express, biology, SERS biosensors, Avidin, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Highly sensitive, symbols, biology.protein, 0210 nano-technology, Plasmonic nanostructures, Biosensor, Raman scattering
Abstract

Abstract We report on the use of soft UV nanoimprint lithography (UV-NIL) for the development of reproducible, millimeter-sized, and sensitive substrates for SERS detection. The used geometry for plasmonic nanostructures is the cylinder. Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces. Indeed, we demonstrated that less than 4 ×106 avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal. Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm 2.

Published
2014
Full Text
View/download PDF

13. Hydrophilic Monolith With Ethylene Glycol-Based Grafts Prepared Via Surface Confined Thiol-Ene Click Photoaddition

Author

Benjamin Carbonnier, Mohamed Guerrouache, Julien Babinot, Inga Tijunelyte, and Gintaras Valincius

Subjects
geography, geography.geographical_feature_category, Monolithic HPLC column, Materials science, Polymers and Plastics, Organic Chemistry, Allylamine, Solvent, chemistry.chemical_compound, chemistry, Electrochromatography, Polymer chemistry, Materials Chemistry, Copolymer, Click chemistry, Monolith, Ethylene glycol
Abstract

Macroporous polymeric monolith bearing thiol-reactive surface functionalities was prepared within micrometer-sized fused silica capillary column by photochemically-driven free radical copolymerization of N-acryloxysuccininimide and ethylene dimethacrylate in the presence of toluene as porogenic solvent, hereafter poly(NAS-co-EDMA), and subsequent surface grafting of allylamine through nucleophilic substitution reaction. The pore surface with pendant allyl moieties was further functionalized via a two-step thiol-ene click reaction with thiol-containing oligo(ethylene glycol) and mercaptoethanol, successively. The surface hydration ability, i.e. hydrophilic character, of the as-obtained monolith was evaluated as a function of the water-content of the liquid fluid environment through electrochromatographic evaluation of the retention properties of the -(O-CH2-CH2)-like surface-functionalized capillary monolith. A major result was that the so called hydrophilic interaction electrochromatographic mode was observed at a given mobile phase composition. An example is given for the separation of phenol-derivatives, with the most hydrophilic one being the most retained. In addition, the hydrophilic interaction based separation allowed for reduced analysis time as compared to the separation observed under reversed-phase separation mode using analogous monolithic stationary phase. Finally, the versatility of the thiol-ene photoaddition approach is demonstrated with an example of C18-like monolith for reversed-phase separation application. (O) 2011 Elsevier Ltd. All rights reserved.

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results