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Background Cystatin C has been proposed as a novel marker of renal function and predictor of cardiovascular risk. The aim of this study was to investigate the role of cystatin C level as a predictor of cardiovascular events in patients with coronary artery disease (CAD). Methods Three hundred and five coronary artery patients were included in this study. Serum cystatin C levels, high-sensitive C-reactive protein (hs-CRP), and oxidative stress were measured. Estimated glomerular filtration rate (eGFR) and the CAD severity score were calculated. Results Cystatin C was correlated with the CAD severity score (r = 0.631, P 50. Every 0.1 mg/l increase in cystatin C, 2 mg/l increase in hs-CRP, 0.2 mmol/l decrease in high-density lipoprotein cholesterol, 13.7 ml/min decrease in eGFR, and 1.51 μmol/l increase in homocysteine caused a 34, 12, 5, and 22% increase in the risk of having CAD, respectively. Conclusion Cystatin C could be a useful laboratory biochemical marker in predicting the severity of CAD. Cystatin C is associated with biochemical atherosclerosis markers such as CRP and homocysteine.

Objectif : developper une technique rapide et fiable de PCR en temps reel pour detecter les polymorphismes du gene de l’angiotensinogene (AGT), comparer les deux methodes de la MS-PCR ( mutagenically separated PCR) et de la PCR en temps reel pour determiner les trois polymorphismes du gene de l’angiotensinogene le M235T, le A (-6)G et le A(-20)C. Methodes : dans cette etude, la methode de PCR en temps reel a ete developpee sur l’automate Roche LightCycler1 avec SYBR Green I. Nous avons utilise dans deux amorces sens et une amorce non-sens. La detection des polymorphismes du gene de l’angiotensinogene a ete realisee par comparaison des courbes de fusion. Resultats : les echantillons d’ADN ont ete analyses par les deux methodes : la PCR en temps reel et la MS-PCR. Aucune difference n’a ete trouvee entre les deux techniques. Discussion : la PCR en temps reel est une methode fiable et rapide pour la detection des polymorphismes du gene de l’AGT le M235T, le A (-6)G et le A(-20)C. Conclusion : cette methode de PCR en temps reel est un test genetique fiable, rapide et qui n’est pas cher et peut etre utilise en pratique pour etudier particulierement les polymorphismes du gene de l’AGT associes a la maladie cardiovasculaire.

Introduction: Oxidative stress is now recognized as being the cause and the consequence of cardiovascular diseases. Objective: The role that oxidative stress parameters and inflammatory markers may play in diabetes and related cardiovascular disease (CVD) among Tunisian coronary diabetic patients. Patients and methods: We measured the erythrocyte glutathione peroxidase (GPX), the superoxide dismutase (SOD) activities and the plasmatic total antioxidant status (TAS) concentration by colorimetric methods, the hs-CRP by immunonephelometry assays. Results: TAS and GPX were significantly decreased among patients compared to the controls (1.14 ± 0.28 mmol/l vs 1.55 ± 0.35 mmol/l; 59.32 ± 10.72 U/gHb vs 149.19 ± 30.95 U/gHb). For the diabetic patients, TAS is correlated positively with hs-CRP (r=0.01, p