3 results on '"Hum, Nicholas"'
Search Results
2. LPS-Induced Inflammation Prior to Injury Exacerbates the Development of Post-Traumatic Osteoarthritis in Mice
- Author
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Mendez, Melanie E, Sebastian, Aimy, Murugesh, Deepa K, Hum, Nicholas R, McCool, Jillian L, Hsia, Allison W, Christiansen, Blaine A, and Loots, Gabriela G
- Subjects
Lipopolysaccharides ,Aging ,LPS ,Physical Injury - Accidents and Adverse Effects ,Medical and Health Sciences ,Mice ,Engineering ,INFLAMMATION ,Osteoarthritis ,RNA SEQUENCING ,Genetics ,Animals ,Humans ,2.1 Biological and endogenous factors ,Aetiology ,mu CT ,ACL ,Arthritis ,Inflammatory and immune system ,X-Ray Microtomography ,Biological Sciences ,Anatomy & Morphology ,Cartilage ,MRL/MpJ ,μCT ,Musculoskeletal ,BONE ,Articular - Abstract
Osteoarthritis (OA) is a debilitating and painful disease characterized by the progressive loss of articular cartilage. Post-traumatic osteoarthritis (PTOA) is an injury-induced type of OA that persists in an asymptomatic phase for years before it becomes diagnosed in ~50% of injured individuals. Although PTOA is not classified as an inflammatory disease, it has been suggested that inflammation could be a major driver of PTOA development. Here we examined whether a state of systemic inflammation induced by lipopolysaccharide (LPS) administration 5-days before injury would modulate PTOA outcomes. RNA-seq analysis at 1-day post-injury followed by micro-computed tomography (μCT) and histology characterization at 6 weeks post-injury revealed that LPS administration causes more severe PTOA phenotypes. These phenotypes included significantly higher loss of cartilage and subchondral bone volume. Gene expression analysis showed that LPS alone induced a large cohort of inflammatory genes previously shown to be elevated in synovial M1 macrophages of rheumatoid arthritis (RA) patients, suggesting that systemic LPS produces synovitis. This synovitis was sufficient to promote PTOA in MRL/MpJ mice, a strain previously shown to be resistant to PTOA. The synovium of LPS-treated injured joints displayed an increase in cellularity, and immunohistological examination confirmed that this increase was in part attributable to an elevation in type 1 macrophages. LPS induced the expression of Tlr7 and Tlr8 in both injured and uninjured joints, genes known to be elevated in RA. We conclude that inflammation before injury is an important risk factor for the development of PTOA and that correlating patient serum endotoxin levels or their state of systemic inflammation with PTOA progression may help develop new, effective treatments to lower the rate of PTOA in injured individuals. © 2020 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research.
- Published
- 2020
3. SOST/Sclerostin Improves Posttraumatic Osteoarthritis and Inhibits MMP2/3 Expression After Injury
- Author
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Chang, Jiun C, Christiansen, Blaine A, Murugesh, Deepa K, Sebastian, Aimy, Hum, Nicholas R, Collette, Nicole M, Hatsell, Sarah, Economides, Aris N, Blanchette, Craig D, and Loots, Gabriela G
- Subjects
Genetic Markers ,Aging ,Physical Injury - Accidents and Adverse Effects ,Inbred C57BL ,Medical and Health Sciences ,Mice ,Engineering ,Models ,Osteoarthritis ,2.1 Biological and endogenous factors ,Animals ,Humans ,Knee ,Aetiology ,SCLEROSTIN ,WNT SIGNALING ,Glycoproteins ,OSTEOPHYTE ,Binding Sites ,MMP ,Tumor Necrosis Factor-alpha ,Arthritis ,Prevention ,Anterior Cruciate Ligament Injuries ,NF-kappa B ,Signal Transducing ,Adaptor Proteins ,Biological Sciences ,Biological ,Anatomy & Morphology ,Recombinant Proteins ,Extracellular Matrix ,Up-Regulation ,Phenotype ,Musculoskeletal ,Bone Morphogenetic Proteins ,Intercellular Signaling Peptides and Proteins ,Matrix Metalloproteinase 2 ,Matrix Metalloproteinase 3 ,SOST - Abstract
Patients with anterior cruciate ligament (ACL) rupture are two times as likely to develop posttraumatic osteoarthritis (PTOA). Annually, there are ∼900,000 knee injuries in the United States, which account for ∼12% of all osteoarthritis (OA) cases. PTOA leads to reduced physical activity, deconditioning of the musculoskeletal system, and in severe cases requires joint replacement to restore function. Therefore, treatments that would prevent cartilage degradation post-injury would provide attractive alternatives to surgery. Sclerostin (Sost), a Wnt antagonist and a potent negative regulator of bone formation, has recently been implicated in regulating chondrocyte function in OA. To determine whether elevated levels of Sost play a protective role in PTOA, we examined the progression of OA using a noninvasive tibial compression overload model in SOST transgenic (SOSTTG ) and knockout (Sost-/- ) mice. Here we report that SOSTTG mice develop moderate OA and display significantly less advanced PTOA phenotype at 16 weeks post-injury compared with wild-type (WT) controls and Sost-/- . In addition, SOSTTG built ∼50% and ∼65% less osteophyte volume than WT and Sost-/- , respectively. Quantification of metalloproteinase (MMP) activity showed that SOSTTG had ∼2-fold less MMP activation than WT or Sost-/- , and this was supported by a significant reduction in MMP2/3 protein levels, suggesting that elevated levels of SOST inhibit the activity of proteolytic enzymes known to degrade articular cartilage matrix. Furthermore, intra-articular administration of recombinant Sost protein, immediately post-injury, also significantly decreased MMP activity levels relative to PBS-treated controls, and Sost activation in response to injury was TNFα and NF-κB dependent. These results provide in vivo evidence that sclerostin functions as a protective molecule immediately after joint injury to prevent cartilage degradation. © 2018 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc.
- Published
- 2018
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