113 results on '"Hui L."'
Search Results
2. Firm-Level Political Risk and the Manipulative Characteristics of Earnings Management
- Author
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Hui L. James, Jurica Susnjara, and Thanh Ngo
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2023
3. Labor Investment Efficiency and CEO Tenure
- Author
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Hui L. James, Pornsit Jiraporn, and Hongxia Wang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2023
4. The Impact of More Able Managers on Corporate Trade Credit
- Author
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Hui L. James, Thanh Ngo, and Hongxia Wang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2023
5. Covenant Violation and Operational Efficiency
- Author
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Hui L. James, Hongxia Wang, and Zhimin Wang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2023
6. Social Capital and the Riskiness of Trade Credit
- Author
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Hui L. James
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2023
7. GARFIELD-AF: risk profiles, treatment patterns and 2-year outcomes in patients with atrial fibrillation in Germany, Austria and Switzerland (DACH) compared to 32 countries in other regions worldwide
- Author
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Haas, S., Camm, J.A., Harald, D., Steffel, J., Virdone, S., Pieper, K., Brodmann, M., Schellong, S., Misselwitz, F., Kayani, G., Kakkar, A.K., Jean-Pierre, B., John Camm, A., Fitzmaurice, D.A., Fox, K.A.A., Gersh, B.J., Goldhaber, S.Z., Shinya, G., Sylvia, H., Werner, H., Mantovani, L.G., Frank, M., Pieper, K.S., Turpie, A.G.G., Martin van Eickels, Verheugt, F.W.A., Fox Bernard, K.A.A., Gersh, J., Hector, L., Luciardi Harry Gibbs, Marianne, B., Frank, C., Antonio Carlos Pereira Barretto, Connolly, S.J., John, E., Ramon, C., Zhi-Cheng, J., Petr, J., Jørn Dalsgaard Nielsen, Hany, R., Pekka, R., Jean-Yves Le Heuzey, Matyas, K., Jitendra Pal Singh Sawhney, Giancarlo, A., Giuseppe, A., Yukihiro, K., Carlos Jerjes Sánchez Díaz, Hugo Ten Cate, Dan, A., Janina, S., Elizaveta, P., Toon Wei Lim, Barry, J., Seil, O., Xavier, V., Marten, R., Jan, S., Pantep, A., Ali, O., Alex, P., Wael Al Mahmeed, David, F., Samuel, Z.G., Dayi Hu Kangning Chen, Yusheng, Z., Huaiqin, Z., Jiyan, C., Shiping, C., Daowen, W., Yuejin, Y., Weihua, L., Hui, L., Yuehui, Y., Guizhou, T., Ping, Y., Yingmin, C., Shenghu, H., Yong, W., Guosheng, F., Xin, L., Tongguo, W., Xiaoshu, C., Xiaowei, Y., Ruiping, Z., Moshui, C., Longgen, X., Ping, C., Yang, J., Ying, G., Xue, L., Zhiming, Y., Praveen Jadhavm Raghava Sarma, Govind, K., Prakash, C., Rasesh Atulbhai Pothiwala, Mohanan Padinhare Purayil, Kamaldeep, C., Veerappa Annasaheb Kothiwale, Bagirath, R., Vinod Madan Vijan, Jitendra, S., Ganapathi, B., Aziz, K., Ramdhan, M., Manojkumar, C., Sunitha, A., Vikas, B., Govindan, V., Debabrata, R., Rajashekhar, D., G Ravi Shankar, A., Sunil, K., Dinesh, J., Kartikeya, B., Vinay, K., Udigala Madappa Nagamalesh, Rajeeve Kumar Rajput, Yukihiro Koretsune Seishu Kanamori, Kenichi, Y., Koichiro, K., Yosuke, K., Keiki, Y., Fumitoshi, T., Yuji, M., Ikuo, M., Hiroo, N., Shinichi, A., Tetsuro, S., Masahiro, M., Hiroyuki, O., Susumu, A., Kei, C., Hiroaki, N., Makoto, T., Takeshi, K., Kunihiko, Y., Hiroshi, A., Takayuki, H., Megumi, O., Shiro, A., Shinichiro, K., Kenshi, K., Takashi, M., Jun, M., Yurika, O., Ryuji, S., Kazuo, G., Kotaro, M., Yoshikuni, H., Hisakazu, S., Hiroo, M., Hitoshi, K., Tsugihiro, N., Tadashi, N., Hidekazu, N., Ryuji, Z., Yoshihisa, F., Akira, Y., Hiroyuki, N., Jun, O., Yasuyuki, K., Kinshiro, M., Yutaka, W., Masanori, Y., Hiromitsu, M., Sumihisa, A., Hajime, K., Satoru, T., Katsumi, S., Hiroki, T., Ichiro, O., Takashi, K., Satoshi, H., Masamichi, G., Takuma, E., Hidetoshi, C., Kazuaki, F., Yuhei, S., Hirokuni, S., Toshihisa, N., Yoshihiko, A., Toshiro, N., Kazuhisa, S., Fumihiro, H., Naoto, Y., Masahiro, K., Toshifumi, T., Munesumi, I., Yoshitake, F., Daisuke, I., Taku, S., Tetsu, I., Norio, I., Koichi, O., Keizo, T., Yutaka, H., Motoshi, T., Hiroto, T., Shinjiro, N., Masaaki, I., Yuichiro, N., Naomasa, M., Ashida, K., Jun, A., Seishiro, M., Osamu, A., Shuji, F., Hirofumi, M., Kazuya, M., Yoshiki, H., Ichiro, S., Kotaro, O., Ichiro, T., Mitsuyuki, A., Toshihide, U., Yoshinori, G., Makoto, I., Shoji, M., Shigeru, M., Hideo, D., Mitsuru, T., Takaaki, K., Shigeo, K., Chiga, O., Masaki, S., Masami, N., Yutaka, K., Yoichi, N., Hiroshi, O., Rikimaru, O., Masato, A., Teruaki, M., Kazuhiko, N., Takafumi, M., Junichi, M., Mitsunori, A., Masako, F., Makoto, O., Tsuneo, F., Toshiya, T., Tenei, K., Hiroshi, K., Mizuho, I., Masahiro, A., Takashi, U., Hironori, O., Masahiko, I., Yoshiki, K., Atsuyuki, N., Shinobu, T., Mitsuhiro, S., Masayuki, N., Kenichiro, I., Motoyuki, I., Taro, M., Masamichi, W., Hiroaki, M., Masato, M., Fumio, O., Teruaki, K., Kuniaki, T., Masaaki, T., Morio, I., Hiroshi, W., Toshihiko, S., Shinya, H., Hiroaki, H., Mitsumoto, H., Michitaka, H., Koichi, M., Hideki, H., Nobuyoshi, S., Yukio, S., Akira, S., Kazuo, N., Tetsuro, Y., Kunio, A., Sen, A., Chiei, T., Saori, M., Hirofumi, K., Masanori, K., Shiro, N., Atsushi, T., Shuta, T., Kazuyuki, S., Akiko, M., Hiroki, S., Jin, N., Taketo, H., Takash, I., Kazuki, S., Kazuya, K., Tomobumi, K., Tsuyoshi, T., Hirosumi, S., Kiyoshi, N., Kenichi, I., Kazuo, M., Tomohiro, S., Takeshi, I., Koichi, K., Hiromichi, K., Tsutomu, T., Mamoru, H., Jisho, K., Akitoshi, S., Yoshihiro, T., Tetsuo, B., Koji, H., Masaaki, H., Koichi, H., Takao, B., Kazuaki, M., Toshihiko, K., Kunihiko, H., Toshihide, K., Akira, N., Eiji, O., Takashi, S., Hiroyoshi, H., Chikako, S., Takashi, Y., Ichiro, M., Kazunori, S., Isamu, N., Ken, T., Osamu, I., Koichi, T., Samu, U., Hirokazu, K., Takuya, O., Seizo, O., Junya, K., Toshihiko, N., Itaru, M., Yoshifusa, M., Yasuyuki, M., Kazuo, T., Hajime, H., Tetsutaro, K., Koji, M., Masaichi, N., Takashi, W., Tomoki, Y., Masato, S., Hidekazu, A., Hisanori, S., Hiroyuki, T., Nobufusa, F., Akira, O., Kentaro, Y., Kenji, A., Taku, Y., Takeaki, K., Shunji, S., Shu, S., Nitaro, S., Masayuki, W., Yosuke, N., Toru, A., Masaki, O., Tetsushi, W., Tomoko, K., Yasuo, S., Takeshi, T., Yoshihito, H., Shinichi, H., Yukihiko, A., Yoshihiro, S., Hirohide, U., Hiroshi, T., Shuichi, T., Naoto, H., Seiichi, M., Hisashi, S., Takuma, A., Yasunobu, S., Yawara, N., Osamu, M., Hideko, I., Katsumasa, N., Masatsugu, N., Kazuo, S., Toshiyuki, F., Nobuhisa, I., Shunichi, N., Kiyoharu, S., Yujin, S., Naoko, O., Teruhiko, K., Hideaki, O., Masato, E., Tsutomu, G., Makoto, H., Emiko, N., Noriyuki, N., Toshizumi, M., Shuichi, S., Katsuhiro, O., Yoko, E., Tsuyoshi, F., Haruhiko, D., Shuichi, K., Sho, N., Yuya, U., Tetsuro, F., Mitsuru, I., Takuo, O., Shunsuke, T., Hideo, I., Norihiko, S., Kiyomitsu, I., Nobuo, W., Masatake, A., Junji, D., Tetsuya, K., Masato, T., Naoya, M., Yasuaki, F., Wataru, F., Susumu, S., Akinori, F., Ryosai, N., Hiroyasu, K., Rei, F., Keijiro, N., Yoji, K., Junya, A., Kiyoshi, Y., Toshio, A., Yasuhiro, S., Tatsuo, H., Yuichiro, K., Yasuhide, S., Yukihiro, S., Shingo, M., Kojuro, M., Yasuko, S., Toyoshi, S., Fumiko, I., Toshiyuki, K., Jaeyoung, K., Hiroshi, Y., Yoichi, T., Yoko Onuki Pearce, Yasuyuki, S., Takayuki, F., Toru, N., Hideaki, K., Yoshiyuki, K., Tetsuji, I., Hironori, M., Yasufumi, M., Masahito, S., Shimato, O., Yutaka, O., Satoshi, U., Kojiro, K., Tatsuo, O., Naoki, M., Koichi, I., Atsushi, I., Tomohiro, Y., Toshihiro, G., Tsukasa, K., Atsushi, S., Etsuo, M., Toshio, T., Hiroshi, S., Shunichi, F., Tomohiro, K., Yoshiyuki, F., Hiroshi, H., Jun, N., Kiichiro, Y., Takuya, I., Takafumi, A., Chi Keong Ching Toon Wei Lim, Kelvin, W., Tan, Y., Seil Oh Hui Nam Park, Woo-Shik, K., Hyeyoung, L., Sung-Won, J., Dae Hyeok Kim, Jun, K., Dongryeol, R., Jaemin, S., Dae-Kyeong, K., Dong Ju Choi, Yong Seog Oh, Myeong-Chan, C., Hack-Lyoung, K., Hui-Kyung, J., Dong-Gu, S., Sang Weon Park, Hoon Ki Park, Sang-Jin, H., Jung Hoon Sung, Hyung-Wook, P., Gi-Byoung, N., Young Keun On, Hong Euy Lim, Jaejin, K., Tae-Joon, C., Taek Jong Hong, Seong Hoon Park, Jung Han Yoon, Nam-Ho, K., Kee-Sik, K., Byung Chun Jung, Gyo-Seung, H., Chong-Jin, K., Sakda Rungaramsin Peerapat Katekangplu, Porames, K., Thanita, B., Wanwarang, W., Pinij, K., Khanchai, S., Waraporn, T., Supalerk, P., Khanchit, L., Doungrat, C., Warangkana, B., Sirichai, C., Songkwan, S., Pisit, H., Seksan, C., Pairoj, C., Boonsert, C., Yingsak, S., Khompiya, K., Piya, M., Sasivimon, J., Ongkarn, K., Armagan Altun Ali Aydinlar, Ramazan, T., Zeki, O., Sadik, A., Durmus Yildiray Sahin, Ozcan, Y., Mehmet Birhan Yilmaz, Hasan, P., Mesut, D., Murat, S., Levent, S., Murat, E., Ertugrul, O., Dursun, A., Florencia Rolandi Adrian Cesar Ingaramo, Gustavo Alberto Sambadaro, Vanina Fernandez Caputi, Sofia Graciela Berman, Pablo, D., Andres Javier Kleiban, Nestor, C., Rodolfo Andres Ahuad Guerrero, Leonel Adalberto Di Paola, Ricardo Dario Dran, Javier, E., Matias Jose Fosco, Victor Alfredo Sinisi, Luis Rodolfo Cartasegna, Oscar Gomez Vilamajo, Jose Luis Ramos, Sonia, S., Gerardo, Z., Diego, C., Guillermo, G., Alberto Alfredo Fernandez, Mario Alberto Berli, Fabian, F., Dário Celestino Sobral Filho Jefferson Jaber, Luciana Vidal Armaganijan, Costantino Roberto Frack Costantini, André, S., Weimar Kunz Sebba Barroso de Souzaem, João David de Souza Neto, José Márcio Ribeiro, Marcelo Silveira Teixeira, Paulo, R., Leonardo, P., Daniel, M., José Carlos Moura Jorge, Adalberto Menezes Lorga Filho, Luiz, B., Marcelo Westerlund Montera, Carlos Henrique Del Carlo, Jamil Abdalla Saad, Fernando Augusto Alves da Costa, Renato, L., Gilson Roberto de Araújo, Euler Roberto Manenti, Jose Francisco Kerr Saraiva, João Carlos Ferreira Braga, Alexandre, N., Carlos, M., Dalton, P., Fernando, R., Gilmar, R., Roberto Álvaro Ramos Filho, Estêvão Lanna Figueiredo, Roberto Vieira Botelho, Cláudio Munhoz da Fontoura Tavares, Helius Carlos Finimundi, Adriano, K., César Cássio Broilo França, Fábio, A., Guido Bernardo Aranha Rosito, João Batista de Moura Xavier Moraes Junior, Rogério Tadeu Tumelero, Lilia, M., Roberto Simões de Almeida, Ney Carter do Carmo Borges, Luís Gustavo Gomes Ferreira, Ramón Corbalán Benjamin Aleck Joseh Stockins Fernandez, Humberto, M., Fernando, L., Martín Larico Gómez, Carlos, A., Carlos, C., Patricio Marin Cuevas, Alejandro, F., Claudio Bugueño Gutiérrez, Juan, A., Sergio Potthoff Cardenas, German, E., Cesar, H., Carlos, R., Germán, A., Gustavo Charme Vilches, Carlos Jerjes Sanchez Diaz Jesus Jaime Illescas Diaz, Raul Leal Cantu, Maria Guadalupe Ramos Zavala, Ricardo Cabrera Jardines, Nilda Espinola Zavaleta, Enrique Lopez Rosas, Guillermo Antonio Llamas Esperón, Gerardo, P., Ernesto Cardona Muñoz, Norberto Matadamas Hernandez, Adolfo Leyva Rendon, Norberto Garcia Hernandez, Manuel de Los Rios Ibarra, Luis Ramon Virgen Carrillo, David Lopez Villezca, Carlos Hernandez Herrera, Juan Jose Lopez Prieto, Rodolfo Gaona Rodriguez, Efrain Villeda Espinosa, David Flores Martinez, Jose Velasco Barcena, Omar Fierro Fierro, Ignacio Rodriguez Briones, Jose Luis Leiva Pons, Humberto Alvarez Lopez, Rafael Olvera Ruiz, Carlos Gerardo Cantu Brito, Eduardo Julian Jose Roberto Chuquiure Valenzuela, Roxana Reyes Sanchez, Alberto Esteban Bazzoni Ruiz, Oscar Martin Lopez Ruiz, Roberto Arriaga Nava, Jesus David Morales Cerda, Pedro Fajardo Campos, Mario Benavides Gonzalez, Marianne Brodmann Kurt Lenz, Claus, H., Johannes, F., Heinz, D., Kurt, H., Andrea, P., Michael, W., Bruno, S., Alfons, G., Wilfried, L., Sabine, E., Peter, K., Josef, S., Heribert, R., Bernhard, S., Luc Capiau Geert Vervoort, Bart, W., Geert, H., Jan, V., Dirk, F., Yohan, B., Marc, D., Olivier, X., Harry, S., John, T., Georges, M., Wim, A., Ivan, B., Michel, B., Stefan, V., Peter, V., Philippe, P., Pascal, G., Tim, B., Philippe, D., Alex, H., Joeri, V., Axel De Wolf Eva Zidkova Petr Jansky, Rudolf, S., Vilma, M., Ondrej, L., Josef, O., Lubos, K., Blazej, R., Richard, F., Jan, H., Ilja, K., Zdenek, M., Hana, B., Ondrej, J., Jana, P., Iveta, P., Vratislav, D., Michaela, H., Petr, P., Petr, R., Jindrich, S., Miroslav, N., Vaclav, D., Katarina, P., Jiri, L., Jørn Nielsen Steen Husted, Helena, D., Ulrik, H., Søren, R., Næstved, S., Arne, B., John, M., Jan, B., Jorgen, S., Ebbe, E., Thomas, L., Michael, B., Jacob, M., Morten, S., Michael, O., Pekka Raatikainen Carmela Viitanen, Franck Paganelli Joël Ohayon, Frédéric, C., Michel, G., Yannick, G., Philippe, L., Jean-Joseph, M., Mohamed Bassel Koujan, André, M., Sylvain, D., Olivier, P., Nicolas, D., Jean-Pierre, C., Maxime, G., Dominique, G., G Lokesh, A., Mathieu, Z., Pierre, A., Emmanuel, E., James, K., Pierre-Yves, F., Jean-Pierre, H., Nestor, L., Gilles, R., Igor, S., Jean-Philippe, N., Marie Hélène Mahagne, Antoine, M., Marc, B., Jean-Baptiste, C., Vincent, N., Frederic, S., Gilles, M., Jean-Paul, B., Bernard, D., Michel, M., Désiré, O., Bernard, C., Joseph, M., Etienne, B., Jean Philippe Brugnaux, Alain, F., Pierre, N., Jean-Baptiste, B., Sebastien Schellong Harald Darius, Georg, K., Andreas, K., Uwe, G., Bernd-Thomas, K., Thomas, S., Jan, P., Enno, E., Heinz-Dieter, Z., Peter, R., Christoph, A., Gerd-Ulrich, H., Holger, M., Andreas, P., Stefan, Z., Wolfgang, E., Guenter, R., Dirk, G., Norbert, L., Petra, S., Henning, W., Cosmas, W., Steffen, S., Toralf, S., Adyeri, B., Maximilian, K., Hans-Hermann, Z., Friedhelm, K., Andreas, C., Sabine, O., Torsten, L., Hermann-Josef, H., Gunter, L., Hans-Walter, B., Gunter, H., Dietrich, R., Joachim, H., Praxis Dres Werner Erdle, Wilfried, D., Janna, D., Karl-Albrecht, R., Reinhold, V., Thomas, M., Peter, M., Uwe, H., Volker, E., Heinz, H., Heinz, L., Volker, L., Heiner, M., Christian, S., Herrmann, L., Thomas, B., Gunter, B., Susanne, K., Karsten, M., Sylvia, B., Muwafeg, A., Hans-Holger, E., Carsten, S., Peter, B., Laszlo, K., Britta, S., Wilhelm, H., Jens-Uwe, R., Andras Vertes Gabor Szantai, Andras, M., Nikosz, K., Zoltan, B., Erno, K., Balazs, G., Ferenc, J., Gizella, J., Sandor, K., Zoltan, L., Zsolt, M., Bela, M., Ebrahim, N., Tamas, H., Peter, P., Gabriella, S., Sandor, V., Andras, N., Gabriella, E., Judit, F., Mihaly, E., Giuliana Martini Leone Maria Cristina, Eros, T., Rita, S., Sophie, T., Giovanni Di Minno, Marco, M., Teresa Maria Caimi, Maria, T., Roberto, C., Daniela, P., Roberto, Q., Franco, C., Raffaele, F., Vincenzo, O., Raffaele, R., Roberto, S., Raimondo De Cristofaro, Giuliana, G., Angelo De Blasio, Jorge Salerno Uriate, Flavia, L., Enrico Maria Pogliani, Grzegorz, B., Michele, A., Antonio, M., Mauro, F., Arturo, R., Luciano, F., Andrea, M., Fabrizio, G., Luca, T., Maria, S., Sergio, N., Paolo, R., Antonio, A., Claudio, B., Filippo, T., Massimo, V., Maria, D., Maria Grazia Bongiorni, Silva, S., Alessandro, C., Corrado, L., Enrico, S., Gaetano, S., Tondo, C., Paolo, G., Carmine, M., Saverio, I., Hugo Ten Cate J, H.R., Andreas, L., Henk, A., Maarten, B., Mathijs, P., Coen van Guldener, Johannes, H., S H K P, R.N., Pieter, H., Walter, H., E Groenemeijer, B., Terpstra, W., Cees, B., L V, A.B., Eivind Berge Per Anton Sirnes, Erik, G., Torstein, H., Knut, E., Arne, H., Gunnar, S., Anders, Ø., Beraki, G., Arne, S., Peter, C., Torbjørn, Ø., Svein Høegh Henrichsen, Jan Erik Otterstad, Janina Stepinska Andrzej Gieroba, Malgorzata, B., Michal, O., Beata, W., Krystyna, L., Jaroslaw, Wieslaw, S., Jerzy, K., Roman, Z., Jaroslaw, H., Lucyna, S., Lech, K., Marcin, G., Piotr, M., Maciej, O., Grzegorz, K., Malgorzata, K., Zbigniew, L., Bozenna, O., Jerzy, L., Elzbieta, Z., Agnieszka, K., Malgorzata, C., Iwona, W., Grzegorz, O., Marek, B., Marcin, O., Grazyna, G., Piotr, R., Grzegorz, S., Ryszard, S., Boguslaw, O., Piotr, K., Krzysztof, G., Krzysztof, C., Jaroslaw, J., Pawel, M., Waldemar, M., Stanislaw, M., Roman, L., Jacek, B., Teresa, R., Grzegorz, R., Ewa, D., Jadwiga, N., Jozef, L., Vera Eltishcheva Roman Libis, Gadel, K., Dmitry, B., Liudmila, E., Alexander, K., Eduard, Y., Dmitry, Z., Olga, B., Olga, M., Evgeniy, M., Konstantin, Z., Tatyana, N., Yulia, M., Elena, P., Konstantin, S., Maria, R., Yulia, S., Alla, K., Konstantin, N., Oksana, Z., Anna, Z., Victor, K., Sergey, P., Maria, P., Anton, E., Elena Aleksandrova Oksana Drapkina, Alexander, V., Oleg, N., Petr, C., Svetlana, R., Mikhail, S., Borys, K., Alexey, U., Xavier Vinolas Pere Alvarez Garcia, Maria Fernanda Lopez Fernandez, Luis Tercedor Sanchez, Salvador Tranche Iparraguirre, Pere Toran Monserrat, Emilio Marquez Contreras, Jordi Isart Rafecas, Juan Motero Carrasco, Pablo Garcia Pavia, Casimiro Gomez Pajuelo, Luis Miguel Rincon Diaz, Luis Fernando Iglesias Alonso, Angel Grande Ruiz, Jordi Merce Klein, Jose Ramon Gonzalez Juanatey, Ines Monte Collado, Herminia Palacin Piquero, Carles Brotons Cuixart, Esther Fernandez Escobar, Joan Bayo, I.L., Cecilia Corros Vicente, Manuel Vida Gutierrez, Francisco Epelde Gonzalo, Carlos Alexandre Almeida Fernandez, Encarnacion Martinez Navarro, Juan Jose Montero Alia, Maria Barreda Gonzalez, Maria Angels Moleiro Oliva, Jose Iglesias Sanmartin, Mercedes Jimenez Gonzalez, Maria Del Mar Rodriguez Alvarez, Juan Herreros Melenchon, Tomas Ripoll Vera, Manuel Jimenez Navarro, Maria Vazquez Caamano, Maria Fe Arcocha Torres, Gonzalo Marcos Gomez, Andres Iniguez Romo, Miguel Angel Prieto Diaz, Mårten Rosenqvist Alexander Wirdby, Centrumkliniken, Jan, L., Kerstin, H., Micael, E., Arnor, E., Ulf, B., Liu, B., Anders, L., Lars-Bertil, O., Mikael, G., Lars, A., Lars, B., Claes, B., Ali, H., Björn, M., Marianne, E., Åke, O., Håkan, L., Peter, S., Katarina, T., Hans, H., Pyotr, P., Fredrik, B., Ingar, T., Milita, C., Jan-Erik, K., Agneta, A., Lennart, M., Johan, E., Jörgen, T., Aida, H., Steen, J., Per, S., Jan Steffel Johann Debrunner, Juerg, H.B., Dipen, S., Iurii Rudyk Vira Tseluyko, Oleksandr, K., Svitlana, Z., Igor, K., Oleksandr, P., Iryna, K., Nestor, S., Yuriy, M., Oleksiy, U., Olena, K., Yevgeniya, S., Oleg, S., Mykola, S., Andriy, Y., Susanna, T., Ivan, F., Will Murdoch Naresh Chauhan, Daryl, G., Louise, L., Ramila, P., Philip, S., Bennett, W., Alex, C., Niranjan, P., Jhittay, P., Andrew, R., S Kainth, M., Karim, L., Kevin, D., Gill, P., Joanna, M., Laura, H., Trevor, G., Helga, W., Cumberlidge, Colin, B., Catherine, B., Kevin, J., Shoeb, S., Richard, C., Bhupinder, S., Willcock, W., Sircar, S., John, C., Gilliand, A., Roman, B., Strieder, E., Peter, H., Anne, W., Michael, S., Graham, K., Bhaskhar, V., Nigel, B., Paul, E., Clark, M., John, B., Jennifer, L., Fisher, E., Tim, F., Richard, K., Neil, P., Elizabeth, A., Michael, A., Ramesh, C., Pete, W., Simon, F., Sue, F., Julian, T., Hasan, C., Gary, T., Dawn, T., Matt, P., Claire, S., Carolyn, P., Mark, R., Angus, J., Helen, S., Hywel, J., Claire, G., Matthew, B., Philip, W., Jehad, A., Simon, W., William, L., Phil, E., Frances, S., Neil, M., Stephen, R., Yvette, S., Richard, W., Philip, P., Paul, W., Preeti, P., Andrew, G., Railton, T., Emyr, D., Jonathan, M., Marc, J., Claire, H., Thompson, R., Bijoy, S., Keith, B., Susan, B., Helen, L., David, R., Ulka, C., Ikram, H., Paul, A., Claire, J., Phil, W., Jane, E., Lisa, G., Janet, G., Alison, M., Poland, K., Conor, M., Warke, A., Paul, C., Burns, D., Smith, R., Kamath, R., Jonathan, W., Ian, H., Stephen, V., Paul, R., Hilary, P., Jayesh, P., Amar, A., Nigel, H., Richard, D., Nigel, D., Catherine, N., Mark, D., Purnima, S., Sophia, G., Charlotte, H., Raife, O., Martin, A., Mira, P., Gordon, I., Shahid, A., Catherine, R., Fiaz, C., Sabrina, K., Stephanie, S., Sharon, P., Warwick, C., Neil, R., Amy Butler Steven Coates, Ben, W., Daniel, J., Steve, W., Diane, S., Toh, W., Mark, B., Melanie, D., David, C., Sarah, D., Ben, F., Nick, H., Henry, C., Jon, S., Tim, M., Salah, E., Diane, G., Justin, W., Richard, V., Karen, F., Rob, H., Kashif, Z., Catherine, L., Rebecca, W., Paul, M., Andre, B., Philip, C., Mike, W., Mark, P., Chaminda, D., Greg, R., James, B., Polly, J., Rajesh, M., Matthew, A., Robin, F., Nicolas, T., Simon, C., Rory, R., Simon, R., Christine, A., Ann, F., Andrew, H., Simon, D., Minnal, N., Iain, M., Jane, G., Phil, S., John, S., Emma, B., Adam Blenkhorn Bhuwanendu Singh, Penny, A., William van Gaal, Walter, A., Philip, T., Ron, L., Jens, K., Andrei, C., Hosen, K., David, E., John, F., Bronte, A., Thanh, P., James, R., David, O., Sang Cheol Bae, Harry, G., Patrick, C., Greg, S., Margaret, A., Maurits, B., Astin, L., John Eikelboom Robert Luton, Milan, G., Amritanshu Shekhar Pandey, Stephen, C., Rolland, L., Philippe, B., Félix, A., Joseph, B., John, H., Germain, P., Miranda du Preez, Bradley, S., Reginald, N., Ripple, D., Tomasz, H., Andrea, L., Ratika, P., James, C., Benoit, C., Brian, R., Jorge, B., Saul, V., Sameh, F., Ahmed Mowafy Azza Katta, Mazen, T., Moustafa, N., Mohamed, S., Seif Kamal Abou Seif, Tarek, K., Ahmed Abd El-Aziz, Nasser, T., Ashraf, R., Atef, E., Mohamed Gamal El Din, Magdi, E., Adel, E., David Kettles Junaid Bayat, Heidi, S., Adrian, H., Ynez, K., Riaz, G., Thayabran, P., Michele, G., Louis van Zyl, Hendrik, T., Andrew, M., Rikus, L., Deon, G., Pindile, M., Siddique, I., Fayzal, A., Johannes, E., Shambu, M., Wessel, O., Rehana, L., Veronica, U., Wael AlAl Mahmeed AbdullahNaeemi, Ghazi, Y., Nooshin, B., Munther, A., Rajan, M., Rupesh, S., Ahmed, N., Mohamed, I., Amrish, A., Mukesh, N., Ehab, M.E., Adel, W., Rajeev, G., Michael Cox Scott Beach, Peter, D., Stephen, F., Kevin, F., Miguel, F., W Michael Kutayli, Annette, Q., Niraj, S., Vance, W., Stephen, M., Mark, A., Edwin, B., Roddy, C., Ted, G., Rodney, I., Jorge, G., Howard, N., Pamela, R., Rajneesh, R., Marcus, W., Daniel, N., Keith, F., Ihsan, H., Robert, M., Sridevi, P., Daniel, T., Charles, T., Moustafa, M., Cas, C., Walter, P., Alisha, O., George, P., Jaspal, G., James, W., and Firas, K.
- Subjects
Vitamin K antagonists ,Atrial fibrillation ,GARFIELD-AF ,Non-vitamin K antagonist oral anticoagulants ,Oral anticoagulation ,Phenprocoumon ,Settore MED/11 - Malattie dell'Apparato Cardiovascolare - Abstract
The Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) is a worldwide non-interventional study of stroke prevention in patients with non-valvular AF.52,080 patients with newly diagnosed AF were prospectively enrolled from 2010 to 2016. 4121 (7.9%) of these patients were recruited in DACH [Germany (n = 3567), Austria (n = 465) and Switzerland (n = 89) combined], and 47,959 patients were from 32 countries in other regions worldwide (ORW). Hypertension was most prevalent in DACH and ORW (85.3% and 75.6%, respectively). Diabetes, hypercholesterolaemia, carotid occlusive disease and vascular disease were more prevalent in DACH patients vs ORW (27.6%, 49.4%, 5.8% and 29.0% vs 21.7%, 40.9%, 2.8% and 24.5%). The use of non-vitamin K antagonist oral anticoagulants (NOACs) increased more in DACH over time. Management of vitamin K antagonists was suboptimal in DACH and ORW (time in therapeutic range of INR ≥ 65% in 44.6% and 44.4% of patients or ≥ 70% in 36.9% and 36.0% of patients, respectively). Adjusted rates of cardiovascular mortality and MI/ACS were higher in DACH while non-haemorrhagic stroke/systemic embolism was lower after 2-year follow-up.Similarities and dissimilarities in AF management and clinical outcomes are seen in DACH and ORW. The increased use of NOAC was associated with a mismatch of risk-adapted anticoagulation (over-and-undertreatment) in DACH. Suboptimal control of INR requires educational activities in both regional groups. Higher rates of cardiovascular death in DACH may reflect the higher risk profile of these patients and lower rates of non-haemorrhagic stroke could be associated with increased NOAC use.
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- 2022
8. P‐117: FLI Structure for R1 Foldable AMOLED Display
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Guang C. Yuan, Shi Shu, Zhao Li, Hai T. Huang, Hui L. Wu, Qi Yao, Tie S. Wang, Chuan X. Xu, Dong H. Wang, Jin X. Xue, and Shuang Sun
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Materials science ,AMOLED ,business.industry ,Color gel ,Optoelectronics ,business - Published
- 2020
9. Imperatorin ameliorates learning and memory deficits through <scp>BDNF</scp> / <scp>TrkB</scp> and <scp>ERK</scp> / <scp>CaMKIIα</scp> / <scp>CREB</scp> signaling in prenatally‐stressed female offspring
- Author
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Yi W. Chen, Ying C. Li, Huan H. Deng, Hui L. Jing, Yan J. Cao, Kai Y. Zhang, Yang Li, Si Z. Xia, Xing X. Zheng, and Yi S. Yue
- Subjects
Pharmacology ,MAPK/ERK pathway ,0303 health sciences ,medicine.medical_specialty ,Offspring ,030302 biochemistry & molecular biology ,Hippocampus ,Tropomyosin receptor kinase B ,biochemical phenomena, metabolism, and nutrition ,Hippocampal formation ,Biology ,CREB ,enzymes and coenzymes (carbohydrates) ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Glucocorticoid receptor ,030220 oncology & carcinogenesis ,Ca2+/calmodulin-dependent protein kinase ,Internal medicine ,medicine ,biology.protein ,bacteria - Abstract
Prenatal stress (PS) can lead to impaired spatial learning and memory in offspring. Imperatorin (IMP) is a naturally occurring furanocoumarin with many pharmacological properties. However, the effects of IMP on cognitive impairment induced by PS and the underlying molecular mechanisms remain unclear. We investigated the protective effect of IMP treatment after PS on learning and memory deficits in female offspring at postnatal 60 days. After treating prenatally-stressed offspring with IMP (15 and 30 mg/kg) for 28 days, we found that IMP increased body weight and ameliorated spatial learning and memory and working memory deficits in female offspring rats. Meanwhile, hippocampal Glu and serum corticosterone levels in prenatally-stressed offspring were significantly decreased after IMP administration. Additionally, IMP treatment significantly increased BDNF, TrkB, CaMKII, and CREB mRNA expression in the hippocampus of offspring rats. Furthermore, PS-mediated induction of RKIP protein and mRNA expression and glucocorticoid receptor protein expression in the hippocampus of offspring rats were significantly decreased by IMP treatment, and the protein expression of BDNF and TrkB and relative levels of p-EKR/ERK, p-CaMKIIα/CaMKIIα, and p-CREB/CREB were remarkably increased after IMP treatment. Taken together, IMP can ameliorate PS-induced learning and memory deficits through BDNF/TrkB and ERK/CaMKIIα/CREB signaling pathway and hypothalamic-pituitary-adrenal axis.
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- 2020
10. Pregnancy in the time of COVID-19: what are the challenges for maternity care?
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Hui, L, Knight, M, Edlow, A, Homer, C, and Danchin, M
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Pregnancy ,Biochemistry (medical) ,Clinical Biochemistry ,Q&A ,COVID-19 ,Humans ,Female ,Maternal Health Services ,AcademicSubjects/MED00530 ,AcademicSubjects/SCI00980 ,AcademicSubjects/SCI01290 ,AcademicSubjects/MED00690 - Published
- 2022
11. Every man has his price? Political corruption and CEO compensation
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Yu Chen, Hui L. James, Jung Chul Park, and Ziyun Yang
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
12. Real-World Effectiveness and Safety of Antipsychotics in Individuals at Clinical High-Risk for Psychosis: Study Protocol for a Prospective Observational Study (ShangHai at Risk for Psychosis-Phase 2)
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Wu G, Gan R, Li Z, Xu L, Tang X, Wei Y, Hu Y, Cui H, Li H, Tang Y, Hui L, Liu X, Li C, Wang J, and Zhang T
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antipsychotics ,subgroup ,Neurosciences. Biological psychiatry. Neuropsychiatry ,Neurology. Diseases of the nervous system ,RC346-429 ,early interventions ,clinical high-risk ,RC321-571 - Abstract
GuiSen Wu,1 RanPiao Gan,1 ZhiXing Li,1 LiHua Xu,1 XiaoChen Tang,1 YanYan Wei,1 YeGang Hu,1 HuiRu Cui,1 HuiJun Li,2 YingYing Tang,1 Li Hui,3 XiaoHua Liu,1 ChunBo Li,1 JiJun Wang,1,4,5 TianHong Zhang1 1Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Psychotic Disorders, Shanghai 200030, People’s Republic of China; 2Florida a & M University, Department of Psychology, Tallahassee, FL 32307, USA; 3Institute of Mental Health, The Affiliated Guangji Hospital of Soochow University, Soochow University, Suzhou 215137, Jiangsu, People’s Republic of China; 4Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai, People’s Republic of China; 5Brain Science and Technology Research Center, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaCorrespondence: TianHong Zhang; JiJun WangShanghai Key Laboratory of Psychotic Disorders (No.13dz2260500), Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, 600 Wanping Nan Road, Shanghai 200030, People’s Republic of ChinaTel +86-21-34773065Fax +86-21-64387986Email jijunwang27@163.comBackground: The clinical high-risk (CHR) state is identified as a critical period for early prevention and intervention during the development of psychosis and early treatment may reduce the risk of conversion to psychosis. However, it remains controversial whether antipsychotics are effective in CHR populations. Limited previous randomised controlled trials of antipsychotic treatment of CHR individuals indicated possible short-term efficacy on psychotic symptoms with unclear long-term effects. To answer this question, it is necessary to establish a high-quality real-world cohort study with large sample size to explore the effectiveness and safety of antipsychotics in CHR individuals.Methods: We plan to consecutively recruit 600 CHR individuals from Shanghai Mental Health Centre in the ongoing SHARP-2 (ShangHai At Risk for Psychosis-Phase 2) project between 2019 and 2022. At baseline, participants will be assessed by the Structured Interview for Prodromal Syndromes, the MATRICS Consensus Cognitive Battery, demographic information, and clinical medication history. They will be followed up in a naturalistic way in which the research team will not prescribe antipsychotics or provide pharmacological consultation. First, CHR participants and their families will be trained to record their medication daily and self-evaluate symptoms through smart-phone application-based assessment and report their information weekly. Second, telephone calls will be arranged monthly so that the researchers are informed about the participants’ symptoms, medications and daily functions. Third, face-to-face interviews will be conducted annually for repeating assessment of baseline. The primary outcomes will include conversion to psychosis and functional outcome (scored with less than 60 in the Global Assessment of Function) at the end of the follow-up period.Conclusion: The current study will improve our knowledge on the effectiveness and safety of the use of antipsychotics at the prodromal phase, and will eventually facilitate optimisation of individualised interventions for psychosis prevention and treatment.Keywords: clinical high-risk, early interventions, antipsychotics, subgroup
- Published
- 2019
13. [Efficacy and adverse effects of Ningmitai Capsules combined with antibiotics in the treatment of chronic prostatitis: A meta-analysis]
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Peng, Wu, Rui, Yan, Deng, Chen, Bing-Hui, L, Ya-Xuan, Zhang, Xin-Yuan, Lu, Bei-Bei, Che, Yue, Bai, and Jin-Ming, Yu
- Subjects
Humans ,Capsules ,Anti-Bacterial Agents ,Prostatitis - Abstract
To study the effect, treatment course and adverse reactions of Ningmitai Capsules (NMT) in the treatment of chronic prostatitis (CP).We searched the CNKI, Wanfang, COMDD and VIP databases, Cochrane Library, PubMed, EMBASE and Chinese academic conference papers for related articles before October 2019 on the treatment of CP with NMT, evaluated the quality of the literature with the Jadad Scale, and conducted a meta-analysis using the Stata14 software.Totally, 20 articles were included in this study, involving 3558 cases of CP, 1807 in the observation group and 1751 as controls. In the treatment of CP, NMT combined with quinolone, tetracycline or macrolide exhibited remarkably better effect than any of the above antibiotics used alone (RR [95% CI]: 1.270 [1.215-1.328], 1.232 [1.132-1.340] and 1.239 [1.130-1.359], respectively) and the combination therapy also showed a higher total efficacy after 2, 4 and 8 weeks of medication (RR [95% CI]: 1.256 [1.185-1.330], 1.245 [1.165-1.330] and 1.244 [1.131-1.369], respectively), though a little lower at 4 and 8 than at 2 weeks. There was no statistically significant difference in the incidence rate of adverse reactions between the NMT combination and antibiotics alone groups (P = 0.441).NMT combined with antibiotics, particularly with quinolone, is superior to antibiotics alone in the treatment of CP, though with no statistically significant difference in the incidence rate of adverse reactions between the two options. The length of medication does not inference the therapeutic effect.
- Published
- 2021
14. Pregnancy in the Time of COVID-19: What Are the Challenges for Maternity Care?
- Author
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Moderator, Hui, L, Experts, Knight, M, Edlow, A, Homer, C, and Danchin, M
- Subjects
Pregnancy ,COVID-19 ,Humans ,Female ,Maternal Health Services ,General Clinical Medicine ,1004 Medical Biotechnology, 1101 Medical Biochemistry and Metabolomics, 1103 Clinical Sciences - Published
- 2021
15. Intraspinal Space-Occupying Lesions in Children: Clinical Features, Neuroimaging and Surgical Outcomes of 27 Cases
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Chang H, Hui L, Ma P, Wang F, Wang Y, Yan J, Jin Z, and Li Y
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medicine.medical_specialty ,Text mining ,Neuroimaging ,business.industry ,Medicine ,Radiology ,Space (commercial competition) ,business - Abstract
Background: Pediatric intraspinal space-occupying lesions are relatively uncommon. However, these lesions can result in neurological disabilities due to misdiagnosis and delayed treatment. The main goal of the present study is to evaluate the clinical and radiological features and treatment options of pediatric intraspinal space-occupying lesions in order to improve the clinical recognition and management. Methods: Clinical data of 27 children with intraspinal space-occupying lesions who underwent surgery treatment in a tertiary-care hospital between 2010 and 2018 were retrospectively reviewed and analyzed. Results: Of these 27 patients, 14 (51.85%) were girls and 13 (48.15%) were boys. The most common age group affected was 10~14 years (62.96%, 9 girls and 8 boys in this age group). The mean age was 10.11 years old. Pain and weakness were the most common clinical symptoms. Preoperative magnetic resonance imaging (MRI) identified intramedullary (10 cases, 37.04%), intradural extramedullary (10 cases, 37.04%) and extradural (7 cases, 25.92%) lesions, respectively. The majority of the lesions were intraspinal tumors (23 cases, 85.19%). The histological diagnosis of tumors included embryonic residual tumors (6 cases, 22.22%), ependymoma (5 cases, 18.52%), primitive neuroectodermal tumors (PNET) (3 cases, 11.11%), schwannomas (2 cases, 7.4%), ganglioneuroma (1 case, 3.7%), Ewing’s sarc (1 case, 3.7%), B-cell non Hodgkin lymphoma (1 case, 3.7%), Hodgkin lymphoma (1 case, 3.7%), chondrosarcoma (1 case, 3.7%), ganglioglioma (1 case, 3.7%), and glioma (1 case, 3.7%). Conclusions: The incidence of pediatric intraspinal space-occupying lesions is low, and the clinical manifestation is lack of specificity. The prognosis for children with malignant tumors is poor and surgical resection is still the primary treatment option.
- Published
- 2021
16. Research on Anti-Scourability of Slope Eroded Soil
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Hui L, Na Ch, Chun Lx, and Yongde K
- Subjects
Mining engineering ,Geology ,atmospheric_science - Abstract
Purpose - Soil aggregates are of great significance to soil and water conservation and ecological environment construction in arid area of northwest district.Methods - Exploring the effects of different vegetation includes types and land use methods on the stability of soil aggregates in the Loess Plateau, and provide reference for the rational use and management of land, also the improvement of soil structure in the region. Select 9 types of samples of 0-30 cm of typical soil plots as the research objects, compare and analyze the particle size index, stability differences and anti-erodibility of soil aggregates under various vegetation cover. Results - The results show that P value, MWD value, GMD value, D value, and AI value of the 0-10cm surface soil all show the maximum value. As the depth increases, the size distribution of the above index values of each soil sample in the 10-20cm and 20-30cm layers is different; P value in the 0-30cm depth layer is linearly positively correlated with the AI value and MWD value, and linearly negatively correlated with the D value. The correlation coefficient R between each variable is in the range of 0.78-0.97, and the D value reflects the Loess Plateau area stability and erosion resistance of soil aggregates better. GMD and MWD value show an exponential relationship, the correlation coefficient R value of 10-20cm height layer is 0.46; AI and MWD value in 0-10cm, 20-30cm height layer have a power function relationship, 10-20cm height layer has a polynomial function, the correlation coefficient R value is 0.97. The scour coefficient of different soil samples has a high degree of dispersion, the maximum CV value is 1.92, and the minimum value is 0.49. Conclusions - The results of this study can provide a theoretical basis for the ecological and hydrological benefit evaluation of slope erosion control and vegetation restoration on the Loess Plateau.
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- 2021
17. lncRNA small nucleolar RNA host gene 20 predicts poor prognosis in glioma and promotes cell proliferation by silencing P21
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Li XS, Shen FZ, Huang LY, Hui L, Liu RH, Ma YJ, and Jin BZ
- Subjects
Clinicopathological ,LncRNA SNHG20 ,Glioma ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,Proliferation - Abstract
Xiang-Sheng Li,1 Fa-Zheng Shen,1 Li-Yong Huang,1 Lei Hui,1 Rui-Hua Liu,1 Yan-Juan Ma,2 Bao-Zhe Jin1 1Department of Neurosurgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453000, Henan Province, People’s Republic of China; 2Department of Emergency, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453000, Henan Province, People’s Republic of China Background: In multiple cancers, long non-coding RNA small nucleolar RNA host gene 20 (lncRNA SNHG20) is generally dysregulated. In the present study, both the biological role and clinicopathological value of lncRNA SNHG20 in glioma are explored.Methods: Real-time PCR was employed to determine lncRNA SNHG20 expression in glioma patients. The prognostic role of expression of lncRNA SNHG20 was evaluated in a retrospective cohort study. In addition, the association between lncRNA SNHG20 expression and the clinicopathological features of glioma patients, such as tumor recurrence, survival status, follow-up time, WHO grade, resection extent, tumor location, Karnofsky performance scale score, cystic change, tumor size, gender and age, was discussed. By constructing and transfecting siRNAs that targeted lncRNA SNHG20 into the glioma U87 cells, the effects of lncRNA SNHG20 on the proliferation and cell cycle of U87 cells were assessed through cell counting kit-8, colony formation and cell cycle assays, respectively. In addition, Western blot and real-time PCR measured the expression levels of P21 and CCNA1 in U87 cells after being transfected with SNHG20 siRNA.Results: Our results suggested the high expression of lncRNA SNHG20 in human glioma tissues compared with normal brain tissues, which was related to recurrence-free survival and poor overall survival in glioma patients. According to the existing retrospective cohort study, high lncRNA SNHG20 expression was associated with tumor size, extent of resection, WHO grade, follow-up time, survival status and recurrence. Besides, knocking down the expression of lncRNA SNHG20 could inhibit the proliferation and colony formation abilities of glioma U87 cells through cell cycle arrest. Consequently, the expression of CCNA1 was inhibited, and the expression of P21 was up-regulated in U87 cells.Conclusion: A high lncRNA SNHG20 expression level predicts the poor prognosis for glioma patients. Moreover, lncRNA SNHG20 can promote glioma proliferation through silencing P21 and thus lncRNA SNHG20 is an independent potential prognostic biomarker for glioma patients. Keywords: lncRNA SNHG20, glioma, clinicopathological, prognosis, proliferation
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- 2019
18. New-onset diabetes mellitus among patients with glomerular diseases
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Hui L. Koh, Maggie Wy Wong, Jason C J Choo, Cynthia C. Lim, Irene Y. J. Mok, and Yok Mooi Chin
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medicine.medical_specialty ,Proteinuria ,business.industry ,Retrospective cohort study ,Odds ratio ,030204 cardiovascular system & hematology ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Methylprednisolone ,Interquartile range ,Internal medicine ,Diabetes mellitus ,Epidemiology ,Internal Medicine ,medicine ,030212 general & internal medicine ,medicine.symptom ,business ,medicine.drug - Abstract
BACKGROUND Glomerulonephritis commonly causes kidney failure. Immunosuppressant treatment may be diabetogenic, but data on hyperglycaemia in glomerulonephritis treated with usual clinical care are scant. AIM To assess the epidemiology, risk factors and outcomes for new-onset diabetes among patients with glomerular disease (NODAG). METHODS A single-centre retrospective cohort of nondiabetic adults diagnosed with glomerulonephritis between January 2011 and July 2015. Clinical, laboratory and pharmacotherapy data were retrieved from electronic medical records. Using modified American Diabetes Association criteria, the primary outcome of NODAG was present if fasting venous glucose was ≥7 mmol/L for at least two readings, HbA1c was ≥6.5% or if patient required antidiabetic medications. Secondary outcomes were end-stage renal disease, cardiovascular disease and death. RESULTS NODAG occurred in 48 patients (10.7%); 22 required antidiabetic medication at median 6.2 (interquartile range 1.7, 20.0) months after glomerulonephritis diagnosis. Patients with NODAG had higher prebiopsy fasting glucose, greater proteinuria and lower fasting high-density lipoprotein cholesterol levels. Methylprednisolone and cyclophosphamide were more commonly used among patients with NODAG. In multivariate logistic regression, greater proteinuria (odds ratio 1.08 (95% confidence interval 1.01, 1.16), P = 0.02) and methylprednisolone use (odds ratio 4.02 (95% confidence interval 1.76, 9.18), P = 0.001) were significantly associated with NODAG, independent of the triglyceride/high-density lipoprotein cholesterol ratio as a surrogate measure of insulin resistance. Median follow up was 39.6 (26.9, 57.2) months. Secondary outcomes were not significantly different in patients with and without NODAG. CONCLUSION Proteinuria and methylprednisolone were associated with incident diabetes among patients with glomerular disease treated with usual care. At-risk patients should be appropriately counselled and monitored for hyperglycaemia.
- Published
- 2019
19. WOOD PLASTIC COMPOSITES MADE FROM CORN HUSK FIBER AND RECYCLED POLYSTYRENE FOAM
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KOAY S. CHUN, NUR MUHAMMAD Y. FAHAMY, CHAN Y. YENG, HUI L. CHOO, PANG MING M., and KIM Y. TSHAI
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Corn husk fiber ,lcsh:TA1-2040 ,Natural fiber composites ,lcsh:Technology (General) ,lcsh:T1-995 ,Alkaline treatment ,lcsh:Engineering (General). Civil engineering (General) ,Recycled polystyrene - Abstract
This research is a preliminary study on the preparation of wood plastic composites (WPC) from corn husk fiber (CHF) and recycled polystyrene foam (rPS). The effects of fiber content and alkaline treatment on tensile, thermal, water absorption and morphological properties of the composites were investigated. The rPS/CHF composites were prepared using melt compounding and compression moulding processes. The results showed that an increase of fiber content increased the tensile strength, modulus, and thermal stability (Td50%) of composites. However, the water absorption of composites increased vastly as the fiber content increases. The addition of more fiber also caused an earlier thermal degradation to composites. Alkaline treatment has improved the tensile strength, modulus, thermal stability (Td10%), and also reduced water absorption of rPS/CHF composites. The WPS prepared from rPS and treated CHF shows better tensile and thermal properties with lower water absorption.
- Published
- 2018
20. Changes in connexin 43 in inflammatory skin disorders: Eczema, psoriasis, and <scp>Steven‐Johnson</scp> syndrome/toxic epidermal necrolysis
- Author
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Hui L. Kwong, David L. Becker, Joyce Siong See Lee, Mandy Li Ling Tan, Chia C. Ang, and Hong L. Tey
- Subjects
Pathology ,medicine.medical_specialty ,psoriasiform ,Connexin ,Inflammation ,gap junction ,Psoriasis ,Medicine ,Research Articles ,dermatitis ,integumentary system ,business.industry ,epidermal necrosis ,General Medicine ,medicine.disease ,Toxic epidermal necrolysis ,Staining ,expression patterns ,Epidermal necrosis ,cardiovascular system ,sense organs ,biological phenomena, cell phenomena, and immunity ,medicine.symptom ,business ,Wound healing ,Research Article ,Spongiosis - Abstract
Background Connexin 43 (Cx43) plays a central role in the inflammatory response and wound healing. Targeting Cx43 expression reduces inflammation in a variety of injuries. The expression pattern of Cx43 has not been described for many inflammatory skin diseases. Objectives To describe the expression patterns of Cx43 in eczema, psoriasis, Steven‐Johnson syndrome/toxic epidermal necrolysis. Methods Archival skin biopsies from patients with eczema, psoriasis, and Steven‐Johnson syndrome/toxic epidermal necrosis were identified and examined, with sister sections stained for Cx43 and imaged by confocal microscopy. All samples were compared to age and site‐matched normal skin controls. Results Epidermal Cx43 is reduced in acute eczema, absent in regions of spongiosis, and is highly elevated in subacute and chronic eczema. In plaque psoriasis, Cx43 is overexpressed in areas with psoriasiform hyperplasia with a fish‐scale‐like appearance but is lost in regions surrounding neutrophil microabscesses. Cx43 staining is strong in the neutrophils within these microabscesses. In SJS/TEN, Cx43 expression is elevated in areas bordering normal tissue but is rapidly lost in areas of keratinocyte necrosis. Conclusions Dynamic changes in Cx43 levels are seen in inflammatory skin diseases and may represent future potential therapeutic targets., Connexin 43 (Cx43) plays a central role in the inflammatory response and wound healing. Targeting Cx43 expression reduces inflammation in a variety of injuries. Here we examine the changes in Cx43 protein levels in the epidermis in eczema, psoriasis, Steven‐Johnson Syndrome/Toxic Epidermal necrolysis.
- Published
- 2021
21. The international Perinatal Outcomes in the Pandemic (iPOP) study:protocol [version 1; peer review: awaiting peer review]
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Stock, SJ, Zoega, H, Brockway, M, Mulholland, RH, Miller, JE, Been, JV, Wood, R, Abok, II, Alshaikh, B, Ayede, AI, Bacchini, F, Bhutta, ZA, Brew, BK, Brook, J, Calvert, C, Campbell-Yeo, M, Chan, D, Chirombo, J, Connor, KL, Daly, M, Einarsdóttir, K, Fantasia, I, Franklin, M, Fraser, A, Håberg, SE, Hui, L, Huicho, L, Magnus, MC, Morris, AD, Nagy-Bonnard, L, Nassar, N, Nyadanu, SD, Iyabode Olabisi, D, Palmer, KR, Pedersen, Lars Henning, Pereira, G, Racine-Poon, A, Ranger, M, Rihs, T, Saner, C, Sheikh, A, Swift, EM, Tooke, L, Urquia, ML, Whitehead, C, Yilgwan, C, Rodriguez, N, Burgner, D, and Azad, MB
- Published
- 2021
22. An Comprehensive Index (CIB) with Combination of Consistency in both Case Control and Cohort Study to Determine the Efficacy of a Biomarker
- Author
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Hui L
- Subjects
Oncology ,medicine.medical_specialty ,Index (economics) ,Consistency (statistics) ,business.industry ,Internal medicine ,medicine ,Biomarker (medicine) ,business ,Cohort study - Abstract
Background: This study aimed to describe biomarkers using the comprehensive index of biomarker (CIB) based on consistency rate in both case control (Youden index, Yen) and cohort study (Crc) to determine the efficacy of a biomarker. Methods: The CIB is the geometric mean of Yen and Crc. The simulated data were generated to observe CIB features of sensitivity, specificity, and receiver operating characteristic (ROC) analysis for biomarkersResults: CIB was found to be related to the CRC values ROC analysis. The higher Spe could indicate better diagnostic power and the higher Sen could indicate better joint action for biomarkers with the same Yen. Although Yen is the common index used to evaluate the effectiveness of a biomarker, unfortunately, the Yen value was significantly larger than CIB value under the moderate Spe, showing overestimation.Conclusion: The CIB with combination of consistency in both case control and cohort study could be more reasonable. The CIB could provide a better understanding of the power of a biomarker and would be better at evaluating biomarkers from new systems or concepts.
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- 2020
23. Tobacco use and other aspects related to smoking among school-going adolescents aged 13-15 years in Malaysia: Analysis of three cross-sectional nationally representative surveys in 2003, 2009 and 2016
- Author
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Chee Cheong Kee, Yoon Ling Cheong, Chien H Teh, Jia H Lim, Kuang H Lim, Hui L Lim, Balvinder S G Pradhaman Singh, Pei P Heng, Sumarni Mohd Ghazali, and Yong K Cheah
- Subjects
Health (social science) ,Tobacco use ,global youth tobacco survey ,medicine.medical_treatment ,Medicine (miscellaneous) ,tobacco use ,Smoking prevalence ,lcsh:RC254-282 ,Quit smoking ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,030225 pediatrics ,Environmental health ,Medicine ,030212 general & internal medicine ,Secondhand smoke ,lcsh:RC705-779 ,school-going adolescents ,business.industry ,Public Health, Environmental and Occupational Health ,lcsh:Diseases of the respiratory system ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Smoking initiation ,Multistage sampling ,Smoking cessation ,business ,Electronic cigarette ,secondhand smoke - Abstract
Introduction Periodic surveys on tobacco use patterns and other aspects of tobacco use among school-going adolescents in Malaysia provide information on the effectiveness of anti-smoking measures implemented. However, such information is limited in Malaysia. We investigated the prevalence of smoking and other related aspects among middle-secondary school students in Malaysia from the years 2003-2016 to fill this gap. Methods We analyzed data from the Global Youth Tobacco Survey (GYTS) 2003, GYTS 2009, and the Tobacco and Electronic Cigarette Survey among Malaysia Adolescents (TECMA) 2016. The surveys employed multistage sampling to select representative samples of adolescents attending secondary school in Malaysia. Data were collected using a pre-validated self-administered anonymous questionnaire adopted from the GYTS. Results Between 2003 and 2016, major changes occurred in which there were reductions in the prevalence of ever smoking, current smoking, and susceptibility to smoking. Reductions were also observed in exposure to SHS in public places and in the home. The proportion of school-going adolescents who support a ban on smoking in public places increased between 2013 to 2016, and there was a significant reduction in the proportion of respondents that were offered 'free' cigarettes by tobacco company representatives. However, there was no difference in the proportion of adolescents who initiated smoking before the age of 10 years and current smokers seeking advice to quit smoking across the time period. Conclusions Our study indicates that the smoking policies and measures have been effective in reducing smoking prevalence, secondhand smoke exposure, and access to cigarettes, among school-going adolescents in Malaysia. However, measures to reduce smoking initiation and increase smoking cessation need to be strengthened to reduce the burden of smoking-related diseases in Malaysia in the long-term.
- Published
- 2020
24. Task Offloading for End-Edge-Cloud Orchestrated Computing in Mobile Networks
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Xiuhua Li, Chuan Sun, Hui L, Junhao We, Qingyu Xiongl, Xiaofei Wang, and Victor C. M. Leun
- Subjects
Mobile edge computing ,Computer science ,Heuristic (computer science) ,business.industry ,Quality of service ,Distributed computing ,020302 automobile design & engineering ,020206 networking & telecommunications ,Cloud computing ,02 engineering and technology ,0203 mechanical engineering ,Server ,0202 electrical engineering, electronic engineering, information engineering ,Resource allocation ,Cache ,Enhanced Data Rates for GSM Evolution ,business - Abstract
Recently, mobile edge computing has received widespread attention, which provides computing infrastructure via pushing cloud computing, network control, and storage to the network edges. To improve the resource utilization and Quality of Service, we investigate the issue of task offloading for End-EdgeCloud orchestrated computing in mobile networks. Particularly, we jointly optimize the server selection and resource allocation to minimize the weighted sum of the average cost. A cost minimization problem is formulated underjoint the constraints of cache resource and communication/computation resource of edge servers. The resultant problem is a Mixed-Integer Non-linear Programming, which is NP-hard. To tackle this problem, we decompose it into simpler subproblems for server selection and resource allocation, respectively. We propose a low-complexity hierarchical heuristic approach to achieve server selection, and a Cauchy-Schwards Inequality based closed-form approach to efficiently determine resource allocation. Finally, simulation results demonstrate the superior performance of the proposed scheme on reducing the weighted sum of the average cost in the network.
- Published
- 2020
25. Malaysian adolescents' exposure to secondhand smoke in the car of their parents/guardians: A nationwide cross-sectional school-based study
- Author
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Sumarni Mohd Ghazali, Hui L Lim, Jia H Lim, Pei P Heng, Chee Cheong Kee, Kuang H Lim, Mohd Z Taib, Balvinder Singh Gill, and Chien H Teh
- Subjects
lcsh:RC705-779 ,Health (social science) ,business.industry ,Tobacco control ,Confounding ,Public Health, Environmental and Occupational Health ,Prevalence ,Medicine (miscellaneous) ,parent/guardian transport ,lcsh:Diseases of the respiratory system ,Logistic regression ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,complex mixtures ,Health promotion ,Environmental health ,Medicine ,School based ,Rural area ,business ,Secondhand smoke ,school-going adolescent ,Research Paper ,secondhand smoke ,smoking status - Abstract
Introduction We investigated the prevalence of children's exposure to secondhand smoke (SHS) in the car of their parents/guardians and the associated factors. Methods A self-administered validated questionnaire was used to obtain data from the nationally representative samples of school-going adolescents aged 11-19 years in Malaysia. Prevalence rates were computed and chi-squared tests and multiple logistic regression were conducted. Results Of the participants, 23.3% reported exposure to SHS at least once in the car of their parents/guardians during the last 7 days before the survey. The prevalence and likelihood of SHS exposure were significantly higher in Malays, descendants of natives of Sabah and Sarawak, schools in rural areas, females, and current smokers. However, age group and knowledge on the harmful effects of SHS were not significant after adjusting for confounding effects. Conclusions A substantial proportion of school-going adolescents were exposed to secondhand smoke in the car of their parents/guardians. This highlights the need for effective tobacco control measures to include health promotion and smoke-free car regulations to be introduced to prevent severe health hazards and to reduce smoking initiation among non-smoking adolescents.
- Published
- 2020
26. Cause Analysis for a New Type of Devastating Flash Flood
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Ball J, Qiuhua L, Jingming H, Bingyao L, Yu T, Hui L, and Liping M
- Subjects
Environmental Engineering ,0406 Physical Geography and Environmental Geoscience, 0905 Civil Engineering - Abstract
This work introduces an unprecedented flash flood that resulted in nine casualties in Shimen Valley, China, 2015. Through field survey and numerical simulation, the causes of the disaster are systematically analyzed, finding that the intense storm, terrain features, and the large woody debris(LWD) played important roles. The intense storm induced fast runoff and, in turn, high discharges as a result of the steep catchment surfaces and channels. The flood flushed LWD and boulders downstream until blockage occurred in a contraction section, forming a debris lake. When the debris dam broke, a dam break wave rapidly propagated to the valley mouth, washing people away. After considering the disaster-inducing factors, measures for preventing similar floods are proposed. The analysis presented herein should help others manage flash floods in mountain areas.
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- 2020
27. The hidden patient: chronic physical morbidity, psychological distress, and quality of life in caregivers of older adults
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Mythily Subramaniam, Min En Tan, Louisa Picco, Jianlin Liu, Siow Ann Chong, Rajeswari Sambasivam, Janhavi Ajit Vaingankar, Restria Fauziana, and Hui L. Ong
- Subjects
Gerontology ,Secondary education ,Younger age ,030214 geriatrics ,business.industry ,media_common.quotation_subject ,Primary education ,Psychological distress ,Care provision ,Mental health ,Neglect ,03 medical and health sciences ,Psychiatry and Mental health ,0302 clinical medicine ,Quality of life (healthcare) ,Medicine ,Geriatrics and Gerontology ,business ,030217 neurology & neurosurgery ,media_common - Abstract
Aim Increasing demands for care provision to older adults require good physical and mental health among caregivers. Few studies have examined the health status and correlates of quality of life among caregivers of older adults. The present study therefore sought to examine the prevalence of chronic physical conditions, psychological distress, and correlates of physical and mental quality of life among caregivers of older adults (≥60 years) in Singapore. Methods Participants were 285 informal caregivers who were providing care to an older relative. Participants were recruited at the Institute of Mental Health, Singapore, and they completed self-report measures on chronic physical morbidity, psychological distress, and physical and mental quality of life. Multiple regression models were constructed to examine correlates of physical and mental quality of life. Results More than half of the caregivers had at least one chronic physical condition (58.6%) and psychological distress (52.6%). Chronic physical morbidity, psychological distress, and secondary education status were associated with lower physical quality of life. Psychological distress, younger age, primary education status, and more time spent caregiving were associated with lower mental quality of life. Conclusion Poor physical and mental health among caregivers may impair their ability to provide adequate care to older adults with progressive medical needs. It is important for medical practitioners not to neglect the physical and mental health of caregivers through continued assessment of chronic physical morbidity, psychological distress, and quality of life.
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- 2018
28. The hidden patient: chronic physical morbidity, psychological distress, and quality of life in caregivers of older adults
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Sambasivam, Rajeswari, Liu, Jianlin, Vaingankar, Janhavi A., Ong, Hui L., Tan, Min‐En, Fauziana, Restria, Picco, Louisa, Chong, Siow Ann, and Subramaniam, Mythily
- Subjects
Adult ,Male ,caregivers ,Singapore ,Health Services for the Aged ,Health Status ,Original Articles ,Middle Aged ,Young Adult ,Cross-Sectional Studies ,quality of life ,Surveys and Questionnaires ,Chronic Disease ,Humans ,Original Article ,Female ,mental health ,Stress, Psychological ,Aged - Abstract
Aim Increasing demands for care provision to older adults require good physical and mental health among caregivers. Few studies have examined the health status and correlates of quality of life among caregivers of older adults. The present study therefore sought to examine the prevalence of chronic physical conditions, psychological distress, and correlates of physical and mental quality of life among caregivers of older adults (≥60 years) in Singapore. Methods Participants were 285 informal caregivers who were providing care to an older relative. Participants were recruited at the Institute of Mental Health, Singapore, and they completed self‐report measures on chronic physical morbidity, psychological distress, and physical and mental quality of life. Multiple regression models were constructed to examine correlates of physical and mental quality of life. Results More than half of the caregivers had at least one chronic physical condition (58.6%) and psychological distress (52.6%). Chronic physical morbidity, psychological distress, and secondary education status were associated with lower physical quality of life. Psychological distress, younger age, primary education status, and more time spent caregiving were associated with lower mental quality of life. Conclusion Poor physical and mental health among caregivers may impair their ability to provide adequate care to older adults with progressive medical needs. It is important for medical practitioners not to neglect the physical and mental health of caregivers through continued assessment of chronic physical morbidity, psychological distress, and quality of life.
- Published
- 2018
29. Molecular characterization of HOXC8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in Liaoning cashmere goat
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Liang Deng, Hui L. Xue, Jiao J. Wang, Dan Guo, Yun L Dang, Yu Y. Cong, Shu H Yang, Rong H. Yin, Yu B. Zhu, Shi Q. Wang, Wen L. Bai, and Ze Y Wang
- Subjects
0301 basic medicine ,Sequence analysis ,Plant Science ,Biology ,Evolution, Molecular ,03 medical and health sciences ,Exon ,Quantitative Trait, Heritable ,Complementary DNA ,Genetics ,medicine ,Capra hircus ,Animals ,Cashmere goat ,Amino Acid Sequence ,Cloning, Molecular ,Genetic Association Studies ,Phylogeny ,Homeodomain Proteins ,Polymorphism, Genetic ,Base Sequence ,integumentary system ,030102 biochemistry & molecular biology ,Goats ,Exons ,Sequence Analysis, DNA ,General Medicine ,Methylation ,DNA Methylation ,Hair follicle ,biology.organism_classification ,030104 developmental biology ,medicine.anatomical_structure ,Insect Science ,CpG Islands ,Animal Science and Zoology ,Bubalus - Abstract
Homeobox protein Hox-C8 (HOXC8) is a member of Hox family. It is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. In the present study, we isolated and characterized a full-length open reading frame of HOXC8 cDNA from the skin tissue of Liaoning cashmere goat, as well as, established a phylogenetic relationship of goat HOXC8 with that of other species. Also, we investigated the effect of methylation status of HOXC8 exon 1 at anagen secondary hair follicle on the cashmere fiber traits in Liaoning cashmere goat. The sequence analysis indicated that the obtained cDNA was 1134-bp in length containing a complete ORF of 729-bp. It encoded a peptide of 242 amino acid residues in length. The structural analysis indicated that goat HOXC8 contained a typical homeobox domain. The phylogenetic analysis revealed that Capra hircus HOXC8 had a closer genetic relationship with that of Ovis aries, followed by Bos Taurus and Bubalus bubalis. The methylation analysis suggested that the methylation degree of HOXC8 exon 1 in anagen secondary hair follicle might be involved in regulating the growth of cashmere fiber in Liaoning cashmere goat. Our results provide new evidence for understanding the molecular structural and evolutionary characteristics of HOXC8 in Liaoning cashmere goat, as well as, for further insight into the role of methylation degree of HOXC8 exon 1 regulates the growth of cashmere fiber in goat.
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- 2017
30. Lumbar Kinematics, Functional Disability and Fear Avoidance Beliefs Among Adults with Nonspecific Chronic Low Back Pain
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Nancy G Jette, Kok Beng Gan, Hui L Lim, Yi L Lim, Devinder Kaur Ajit Singh, and Sabarul A Mokhtar
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,disability evaluation ,Clinical & Basic Research ,lcsh:Medicine ,Kinematics ,Lumbar vertebrae ,03 medical and health sciences ,0302 clinical medicine ,Lumbar ,Physical medicine and rehabilitation ,medicine ,low back pain ,030222 orthopedics ,business.industry ,lcsh:R ,General Medicine ,lumbar vertebrae ,Low back pain ,fear ,Oswestry Disability Index ,Chronic low back pain ,medicine.anatomical_structure ,Functional disability ,kinematics ,Physical therapy ,medicine.symptom ,business ,Range of motion ,030217 neurology & neurosurgery - Abstract
Objectives: This study aimed to examine correlations between lumbar kinematics, functional disability and fear avoidance beliefs among adults with nonspecific chronic low back pain (LBP). Methods: This cross-sectional study was conducted between March and December 2014. A total of 32 adults diagnosed with nonspecific chronic LBP were recruited from outpatients attending either an orthopaedic clinic at a university hospital or a private physiotherapy clinic in Malaysia. Lumbar kinematics were measured using sensors attached at the first lumbar (L1) and second sacral (S2) vertebrae levels. The Oswestry Disability Index (ODI) and Fear-Avoidance Beliefs Questionnaire (FABQ) were used to assess degree of functional disability and fear avoidance beliefs, respectively. Results: For maximum range of motion, positive correlations were observed between ODI scores and right lateral flexion and right rotation ( P = 0.01 each), although there was a negative correlation with left rotation ( P = 0.03). With maximum angular velocity, ODI scores were positively correlated with right and left lateral flexion L1 ( P = 0.01 and
- Published
- 2016
31. The loss of ATRX/DAXX complex disturbs rDNA heterochromatinization and promotes development of glioma
- Author
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Jing Shen, Yao Zhang, Nan Wang, Qi Jiang, Xing-Wei Huang, Xiang Rong Cheng, Li N, Wei Y, Lei Lei, Hu X, and Hui L
- Subjects
Histone ,Death-associated protein 6 ,biology ,Transcription (biology) ,DNA methylation ,RNA polymerase I ,biology.protein ,Epigenetics ,Ribosomal DNA ,ATRX ,Cell biology - Abstract
BackgroundRibosomal DNA (rDNA) transcription by the RNA polymerase I (Pol I) is a rate-limited step for ribosome synthesis, which is critical for cell growth, cell differentiation, and tumorigenesis. Meanwhile rDNA transcription is modulated by DNA methylation and histone epigenetic modification. Though with great progress in epigenetic research recently, it still remains much uncertain about the relationship of histone variant epigenetic modification and rDNA transcription.ResultsIn this study, epigenetic profiles of silent rDNA in next-generation sequencing datasets were examined. We found that the chaperone of histone variant H3.3, the alpha-thalassemia/mental retardation X-linked syndrome protein (ATRX)/death domain-associated protein (DAXX) complex, and methyltransferase SET domain bifurcated 1 (Setdb1, also known as ESET) help maintain H3.3K9me3 modifications among the promoter and coding regions of silent rDNA. Our experiments further confirmed that DAXX depletion leads to the conversion of silent rDNA into upstream binding factor-bound active rDNA and the release of rDNA transcriptional potency. Support for this model is provided by data from a low-grade glioma in which ATRX is lost and a higher level of ribosomal biosynthesis, nucleolus activity, and proliferation are observed.ConclusionsWe demonstrate a model of epigenetic regulation for rDNA with roles for the ATRX/DAXX complex and H3.3/H3K9me3 modifications identified. Thus, loss of ATRX/DAXX may represent a driving force for tumorigenesis due to its contribution to the release of rDNA transcriptional potency.
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- 2019
32. An Integrated Analysis of Cashmere Fineness lncRNAs in Cashmere Goats
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Dan Guo, Hui L. Xue, Ze Y Wang, Yuan Y. Zheng, Bo J. Li, Tai Y. Hui, Sheng D. Sheng, Jia M. Sun, Wen L. Bai, Chang Yue, and Su L. Guo
- Subjects
0301 basic medicine ,lcsh:QH426-470 ,Intermediate filament cytoskeleton ,RNA-Seq ,Computational biology ,Biology ,Genome ,Article ,differently expressed genes ,03 medical and health sciences ,0302 clinical medicine ,lncRNA ,Gene expression ,Genetics ,Animals ,Gene Regulatory Networks ,lncRNA–targets ,RNA, Messenger ,KEGG ,Gene ,Genetics (clinical) ,Skin ,Messenger RNA ,Goats ,cashmere fineness ,RNA ,Computational Biology ,High-Throughput Nucleotide Sequencing ,Lipid Metabolism ,lcsh:Genetics ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,RNA, Long Noncoding ,RNA-seq ,Hair Follicle - Abstract
Animal growth and development are regulated by long non-coding RNAs (lncRNAs). However, the functions of lncRNAs in regulating cashmere fineness are poorly understood. To identify the key lncRNAs that are related to cashmere fineness in skin, we have collected skin samples of Liaoning cashmere goats (LCG) and Inner Mongolia cashmere goats (MCG) in the anagen phase, and have performed RNA sequencing (RNA-seq) approach on these samples. The high-throughput sequencing and bioinformatics analyses identified 437 novel lncRNAs, including 93 differentially expressed lncRNAs. We also identified 3,084 differentially expressed messenger RNAs (mRNAs) out of 27,947 mRNAs. Gene ontology (GO) analyses of lncRNAs and target genes in cis show a predominant enrichment of targets that are related to intermediate filament and intermediate filament cytoskeleton. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, sphingolipid metabolism is a significant pathway for lncRNA targets. In addition, this is the first report to reveal the possible lncRNA&ndash, mRNA regulatory network for cashmere fineness in cashmere goats. We also found that lncRNA XLOC_008679 and its target gene, KRT35, may be related to cashmere fineness in the anagen phase. The characterization and expression analyses of lncRNAs will facilitate future studies on the potential value of fiber development in LCG.
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- 2019
33. Elevated Plasma D-Dimer in Adult Community-Acquired Pneumonia Patients is Associated with an Increased Inflammatory Reaction and Lower Survival
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Hui L Li, Zhen Z Li, Xiao Y Zhu, Hao Chen, Jing Xu, Yan L Ge, Hua L Yu, Hai F Zhang, Nan Wang, Chuan S Su, Ai S Fu, Hong L Li, Xue Zhang, Cong H Liu, and Hong Y Wang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Positive correlation ,Gastroenterology ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Fibrin Fibrinogen Degradation Products ,Community-acquired pneumonia ,Internal medicine ,D-dimer ,Medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,Inflammation ,Adult patients ,Receiver operating characteristic ,business.industry ,Retrospective cohort study ,Emergency department ,Pneumonia ,Middle Aged ,medicine.disease ,Prognosis ,Icu admission ,Community-Acquired Infections ,C-Reactive Protein ,Female ,business ,Procalcitonin ,Biomarkers - Abstract
BACKGROUND Community-acquired pneumonia (CAP) is a common infectious disease. Inflammatory reaction and prognosis assessment in adult CAP patients are useful for CAP site of care decisions. Most CAP patients were diagnosed in an out-patient or emergency department, so a simple, cheap and rapidly available measurement to assess inflammatory reaction and prognosis has the prospect of broad application. The aim of this study was to investigate the usefulness of plasma D-Dimer in assessment of inflammatory reaction and prognosis in adult CAP patients. METHODS A retrospective study was conducted. All adult patients with a primary diagnosis of CAP were included and were evaluated by peripheral plasma D-Dimer test. All of the measurement data were analyzed with paired t-test and the enumeration data were analyzed with χ2 test. Correlative factor analysis was performed between D-Dimer levels and serum inflammatory markers (WBC, hs-CRP, PCT) and prognostic indexes (ICU admission and 30-day mortality). Receiver operating characteristic curves (ROC) were used to evaluate the sensitivity and specificity of D-Dimer in predicting ICU admission and/or 30-day mortality. RESULTS One hundred fifty patients were included. Compared with non-D-Dimer elevated group, serum inflammatory markers (WBC, hs-CRP, PCT) and prognostic indexes (ICU admission and 30-day mortality) were elevated in the D-Dimer elevated group (p < 0.05). D-Dimer had positive correlation with serum inflammatory markers (WBC, hs-CRP, PCT), the rates of ICU admission and 30-day mortality, and scores of CURB-65. The AUC of ROC curve of D-Dimer was 0.880 (95% CI 0.823 to 0.936), the sensitivity was 80.4% and specificity was 79.8%, D-Dimer levels are superior to hs-CRP and PCT in predicting 30-day mortality and/or ICU admission according to AUCs of the ROC curves. CONCLUSIONS Elevated plasma D-Dimer in adult CAP patients is associated with an increased inflammatory reaction and ICU admission and 30-day mortality. It can be a simple, cheap, and rapidly available measurement to assess inflammatory reaction and prognosis in adult CAP patients.
- Published
- 2019
34. Elevated Red Blood Cell Distribution Width Combined White Blood Cell in Peripheral Blood Routine Have a Better Sensitivity than CURB-65 Scores in Predicting ICU Admission and Mortality in Adult Community-Acquired Pneumonia Patients
- Author
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Hua L Yu, Hong L Li, Zhen Z Li, Hui L Li, Hong Y Wang, Chuan S Su, Cong H Liu, Al-Masud Rana, Xue Zhang, Hai F Zhang, Yan L Ge, Nan Wang, Ruhul A Hassan, Xiao Y Zhu, Ai S Fu, Jing Xu, and Jia B Zhang
- Subjects
Adult ,Erythrocyte Indices ,Male ,China ,030213 general clinical medicine ,medicine.medical_specialty ,Critical Care ,Pneumonia severity index ,Severity of Illness Index ,General Biochemistry, Genetics and Molecular Biology ,Procalcitonin ,Leukocyte Count ,03 medical and health sciences ,0302 clinical medicine ,Community-acquired pneumonia ,Internal medicine ,Severity of illness ,medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,Univariate analysis ,business.industry ,Retrospective cohort study ,Red blood cell distribution width ,Pneumonia ,Middle Aged ,medicine.disease ,CURB-65 ,Community-Acquired Infections ,Female ,business - Abstract
pstrongemBackground/em/strong: Scoring systems including CURB-65 and Pneumonia Severity Index (PSI) and novel or traditional biomarkers including procalcitonin (PCT) and c-reactive protein (CRP) are very significant for understanding the severity and prognosis in community-acquired pneumonia (CAP) patients, while prognostic items are useful for CAP prognostication and point-of-care decisions. The aim of this study was to investigate the usefulness of peripheral blood routine items in predicting ICU admission and 30-day mortality in CAP patients./ppstrongemMethods/em/strong: A retrospective study was conducted. All adult patients with a primary diagnosis of CAP were included and peripheral blood routine tests were evaluated. Univariate analysis and multivariate logistic regression analysis were used to explore association of risk factors with 30-day mortality among CAP patients. Receiver operating characteristic curves (ROC) were used to evaluate the sensitivity and specificity of peripheral blood routine items and compared with CURB-65 scores in predicting ICU admission and/or 30-day mortality./ppstrongemResults/em/strong: One hundred fifty patients were included and compared with non-ICU admission patients. There was a statistically significant difference in age, co-existing illness, RDW, WBC, and CURB-65 scores ranking in ICU admission patients (p0.05). In multivariate logistic regression analysis, we found RDW, WBC, and CURB-65 ≥ 3 scores increased the risk of 30-day mortality by 4.01, 1.65, and 3.43 times, respectively. The area under the curve (AUC) of ROC curves of RDW combined with WBC and CURB-65 was 0.786 (95% CI 0.701 to 0.876) and 0.836 (95% CI 0.764 to 0.908), respectively and the sensitivity was 84.0% and 60.0%, respectively, and the specificity 66.7% and 93.7%, respectively./ppstrongemConclusions/em/strong: Elevated RDW and WBC increased mortality in adult CAP patients, RDW combined with WBC had a better sensitivity than CURB-65 scores in predicting ICU admission and/or mortality in CAP patients./p.
- Published
- 2019
35. Imperatorin ameliorates learning and memory deficits through BDNF/TrkB and ERK/CaMKIIα/CREB signaling in prenatally-stressed female offspring
- Author
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Xing X, Zheng, Kai Y, Zhang, Ying C, Li, Yi W, Chen, Yi S, Yue, Si Z, Xia, Yang, Li, Huan H, Deng, Hui L, Jing, and Yan J, Cao
- Subjects
Memory Disorders ,Pregnancy ,Brain-Derived Neurotrophic Factor ,Furocoumarins ,Animals ,Learning ,Female ,Stress, Psychological ,Rats - Abstract
Prenatal stress (PS) can lead to impaired spatial learning and memory in offspring. Imperatorin (IMP) is a naturally occurring furanocoumarin with many pharmacological properties. However, the effects of IMP on cognitive impairment induced by PS and the underlying molecular mechanisms remain unclear. We investigated the protective effect of IMP treatment after PS on learning and memory deficits in female offspring at postnatal 60 days. After treating prenatally-stressed offspring with IMP (15 and 30 mg/kg) for 28 days, we found that IMP increased body weight and ameliorated spatial learning and memory and working memory deficits in female offspring rats. Meanwhile, hippocampal Glu and serum corticosterone levels in prenatally-stressed offspring were significantly decreased after IMP administration. Additionally, IMP treatment significantly increased BDNF, TrkB, CaMKII, and CREB mRNA expression in the hippocampus of offspring rats. Furthermore, PS-mediated induction of RKIP protein and mRNA expression and glucocorticoid receptor protein expression in the hippocampus of offspring rats were significantly decreased by IMP treatment, and the protein expression of BDNF and TrkB and relative levels of p-EKR/ERK, p-CaMKIIα/CaMKIIα, and p-CREB/CREB were remarkably increased after IMP treatment. Taken together, IMP can ameliorate PS-induced learning and memory deficits through BDNF/TrkB and ERK/CaMKIIα/CREB signaling pathway and hypothalamic-pituitary-adrenal axis.
- Published
- 2018
36. Prevalence and Associated Factors of Cancer-Related Fatigue Among Cancer Patients in Eastern China
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Shu J. Qian, Xiao J. Zhang, Ke J. Chen, Li Tian, Yan Hu, Lu Lin, and Hui L. Li
- Subjects
endocrine system ,Global Health and Cancer ,Cancer Research ,medicine.medical_specialty ,Multivariate analysis ,business.industry ,Eastern china ,Questionnaire ,Cancer ,Physical exercise ,Effective management ,medicine.disease ,Odds ,03 medical and health sciences ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Physical therapy ,030212 general & internal medicine ,medicine.symptom ,business ,Cancer-related fatigue ,hormones, hormone substitutes, and hormone antagonists - Abstract
Background. Cancer-related fatigue (CRF) is a subjective and distressing symptom, and its associated factors in developing countries remain ambiguous. The goal of this study was to determine the prevalence of and factors associated with CRF among cancer patients in China. Methods. This study was designed as a cross-sectional study to determine the prevalence of and factors associated with CRF among cancer patients in eastern China, regardless of their diagnoses. Data were collected by using a questionnaire survey (including demographic information and brief fatigue inventory) after informed written consent was obtained. A chi-square test was used to analyze the correlations between single categorical factors and CRF, and multiple logistic regression analysis was used to evaluate the associations of potential risk factors with the presence of CRF. Results. Out of a total population of 1,938 cancer patients, 1,749 had completed the study questionnaire; 52.07% (n = 904) reported clinically significant fatigue (score ≥4 on Brief Fatigue Inventory). Four hundred twenty-seven (48.47%) patients younger than age 58 years (the median age) and 475 (55.69%) patients age 58 years or older reported clinically significant fatigue. In multivariate analysis, higher sleep quality (p < .01) was negatively associated with CRF, whereas never engaging in physical exercise (p < .01) and higher clinical stage of cancer (p < .01) were positively associated factors that could increase the odds of CRF. Conclusion. The results of this study suggest that effective management of the two changeable contributing factors of CRF may reduce CRF and thus could be used as references for CRF management.
- Published
- 2016
37. New-onset diabetes mellitus among patients with glomerular diseases
- Author
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Cynthia C, Lim, Maggie W Y, Wong, Hui L, Koh, Yok M, Chin, Irene Y J, Mok, and Jason C J, Choo
- Subjects
Adult ,Blood Glucose ,Male ,Middle Aged ,Kidney ,Proteinuria ,Glomerulonephritis ,Logistic Models ,Diabetes Mellitus, Type 2 ,Risk Factors ,Hyperglycemia ,Humans ,Hypoglycemic Agents ,Kidney Failure, Chronic ,Female ,Aged ,Retrospective Studies - Abstract
Glomerulonephritis commonly causes kidney failure. Immunosuppressant treatment may be diabetogenic, but data on hyperglycaemia in glomerulonephritis treated with usual clinical care are scant.To assess the epidemiology, risk factors and outcomes for new-onset diabetes among patients with glomerular disease (NODAG).A single-centre retrospective cohort of nondiabetic adults diagnosed with glomerulonephritis between January 2011 and July 2015. Clinical, laboratory and pharmacotherapy data were retrieved from electronic medical records. Using modified American Diabetes Association criteria, the primary outcome of NODAG was present if fasting venous glucose was ≥7 mmol/L for at least two readings, HbA1c was ≥6.5% or if patient required antidiabetic medications. Secondary outcomes were end-stage renal disease, cardiovascular disease and death.NODAG occurred in 48 patients (10.7%); 22 required antidiabetic medication at median 6.2 (interquartile range 1.7, 20.0) months after glomerulonephritis diagnosis. Patients with NODAG had higher prebiopsy fasting glucose, greater proteinuria and lower fasting high-density lipoprotein cholesterol levels. Methylprednisolone and cyclophosphamide were more commonly used among patients with NODAG. In multivariate logistic regression, greater proteinuria (odds ratio 1.08 (95% confidence interval 1.01, 1.16), P = 0.02) and methylprednisolone use (odds ratio 4.02 (95% confidence interval 1.76, 9.18), P = 0.001) were significantly associated with NODAG, independent of the triglyceride/high-density lipoprotein cholesterol ratio as a surrogate measure of insulin resistance. Median follow up was 39.6 (26.9, 57.2) months. Secondary outcomes were not significantly different in patients with and without NODAG.Proteinuria and methylprednisolone were associated with incident diabetes among patients with glomerular disease treated with usual care. At-risk patients should be appropriately counselled and monitored for hyperglycaemia.
- Published
- 2018
38. Prevalence and factors associated with smoking among adults in Malaysia: Findings from the National Health and Morbidity Survey (NHMS) 2015
- Author
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Muhammad Fadhli Mohd Yusoff, Kuang K Lim, Chien H Teh, Kar H Chong, Chee Cheong Kee, Hui L Lim, Sayan Pan, Kuang H Lim, Sumarni Mohd Ghazali, and Miaw Yn Ling
- Subjects
Health (social science) ,Ethnic group ,Medicine (miscellaneous) ,Smoking prevalence ,Logistic regression ,01 natural sciences ,lcsh:RC254-282 ,smoking ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Malaysian adults ,030212 general & internal medicine ,0101 mathematics ,NHMS ,National health ,lcsh:RC705-779 ,business.industry ,010102 general mathematics ,Public Health, Environmental and Occupational Health ,social-demographic factors ,Validated questionnaire ,lcsh:Diseases of the respiratory system ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Educational attainment ,Younger adults ,business ,Demography ,Research Paper - Abstract
Introduction The continuous monitoring of smoking prevalence and its associated factors is an integral part of anti-smoking programmes and valuable for the evaluation of the effectiveness of anti-smoking measures and policies. This study aimed at determining prevalence of smoking and identifying socio-demographic factors associated with smoking among adults in Malaysia aged 15 years and over. Methods This is a cross-sectional study with a representative sample of 21 445 adults in Malaysia, aged 15 years and over, selected via a stratified, two-stage proportionate-to-size sampling method. Data were obtained from face-to-face interviews by trained research assistants, using a standard validated questionnaire. Multivariable logistic regression was performed to determine socio-demographic factors associated with smoking among Malaysians. Results The overall prevalence of smoking was 22.8% (95% CI: 21.9-23.8%), with males having a significantly higher prevalence compared to females (43.0%, 95% CI: 41.1-44.6 vs 1.4%, 95% CI: 1.1-1.7). The highest smoking prevalence was observed among other ethnicities (35.7%), those aged 25-44 years (59.3%), and low educational attainment (25.2%). Males, those with lower educational attainment and Malays were significantly associated with smoking. Conclusions The prevalence of smoking among Malaysians, aged 15 years and over, remains high despite the implementation of several anti-smoking measures over the past decades. Specially tailored anti-smoking policies or measures, particularly targeting males, the Malays, younger adults and those with lower educational attainment, are greatly warranted to reduce the prevalence of smoking in Malaysia.
- Published
- 2018
39. Surgical outcome of posterior fixation, including fractured vertebra, for thoracolumbar fractures
- Author
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Hui L. Yang, Zhong T. Liu, Quan M. Zhao, and Xiao F. Gu
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Kyphosis ,Lumbar vertebrae ,medicine.disease ,Surgery ,Psychiatry and Mental health ,Fixation (surgical) ,medicine.anatomical_structure ,Thoracic vertebrae ,medicine ,Deformity ,Internal fixation ,Spinal canal ,Neurology (clinical) ,medicine.symptom ,business ,Vertebral column - Abstract
Thoracolumbar fractures are among the most common type of traumatic spine fractures. Surgical treatment is necessary if stability or neurological function is impaired.1 Restoration of the vertebral column stability and decompression of the spinal canal are the main goals of surgical treatment.2 Posterior short-segment fixation has the advantage of using a less extensive approach with less blood loss and complications without compromising the quality of stabilization, and spares healthy mobile segments in fusion, and thus preserves mobility.3 Traditional short-segment fixation (TSSF) is a widely used technique in the treatment of unstable thoracolumbar fractures. It not only provides distraction and compression forces that facilitate 3-dimensional correction and firm fixation, but it also preserves motion segments.4 It can offer several advantages: immediate stability, restoration of the vertebral height and deformity angle, and prevention of late neurological injury.5 However, TSSF has been associated with a high rate of failure.6 The latter is usually associated with implant failure and loss of reduction of kyphosis. Posterior fixation including the fractured vertebra (PFFV) is a novel idea used to overcome the shortcomings of TSSF. The technique avoids the suspension effect and quadrilateral effect, dispensing the internal fixing load and reducing the correction degree losses and internal fixation fractures, and it shows good efficacy in clinical application.7 However, there are still only a few randomized controlled studies7 and the relevant theory requires further investigation. The purpose of this study is to compare the clinical effectiveness of PFFV and TSSF in the treatment of thoracolumbar fracture.
- Published
- 2015
40. LncRNAs in Secondary Hair Follicle of Cashmere Goat: Identification, Expression, and Their Regulatory Network in Wnt Signaling Pathway
- Author
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Yu B. Zhu, Yun L Dang, Su J. Zhao, Ze Y Wang, Hui L. Xue, Shi Q. Wang, Liang Deng, Wei Wang, Yan X. Zhu, Wen L. Bai, Dan Guo, Rong H. Yin, and Yu Y. Cong
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Gene regulatory network ,Bioengineering ,Biology ,03 medical and health sciences ,Internal medicine ,microRNA ,Gene expression ,medicine ,Cashmere goat ,Animals ,Gene Regulatory Networks ,Gene ,Wnt Signaling Pathway ,Expressed sequence tag ,integumentary system ,Goats ,Wnt signaling pathway ,Hair follicle ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Animal Science and Zoology ,Female ,RNA, Long Noncoding ,Hair Follicle ,Biotechnology - Abstract
Long noncoding RNAs (lncRNAs) are a novel class of eukaryotic transcripts. They are thought to act as a critical regulator of protein-coding gene expression. Herein, we identified and characterized 13 putative lncRNAs from the expressed sequence tags from secondary hair follicle of Cashmere goat. Furthermore, we investigated their transcriptional pattern in secondary hair follicle of Liaoning Cashmere goat during telogen and anagen phases. Also, we generated intracellular regulatory networks of upregulated lncRNAs at anagen in Wnt signaling pathway based on bioinformatics analysis. The relative expression of six putative lncRNAs (lncRNA-599618, -599556, -599554, -599547, -599531, and -599509) at the anagen phase is significantly higher than that at telogen. Compared with anagen, the relative expression of four putative lncRNAs (lncRNA-599528, -599518, -599511, and -599497) was found to be significantly upregulated at telogen phase. The network generated showed that a rich and complex regulatory relationship of the putative lncRNAs and related miRNAs with their target genes in Wnt signaling pathway. Our results from the present study provided a foundation for further elucidating the functional and regulatory mechanisms of these putative lncRNAs in the development of secondary hair follicle and cashmere fiber growth of Cashmere goat.
- Published
- 2017
41. A lncRNA-H19 transcript from secondary hair follicle of Liaoning cashmere goat: Identification, regulatory network and expression regulated potentially by its promoter methylation
- Author
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Ze Y Wang, Yan X. Zhu, Shi Q. Wang, Hui L. Xue, Yu Y. Cong, Yu B. Zhu, Qian Jiao, Su J. Zhao, Dan Guo, Wen L. Bai, and Rong H. Yin
- Subjects
0301 basic medicine ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,microRNA ,Genetics ,medicine ,Cashmere goat ,Animals ,Animal Fur ,Promoter Regions, Genetic ,Gene ,Base Composition ,integumentary system ,Base Sequence ,Goats ,Promoter ,General Medicine ,Methylation ,DNA Methylation ,Hair follicle ,female genital diseases and pregnancy complications ,Long non-coding RNA ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Gene Expression Regulation ,embryonic structures ,RNA, Long Noncoding ,Hair Follicle ,Cytosine - Abstract
The H19 transcript (imprinted maternally expressed transcript) is well-known as long noncoding RNA (lncRNA), and it is thought to be associated with the inductive capacity of dermal papilla cells for hair-follicle reconstruction. In this study, we isolated and characterized a lncRNA-H19 transcript from the secondary hair follicle of Liaoning cashmere goat. Also, we investigated its transcriptional pattern and methylation status of H19 gene in secondary hair follicle of this breed during different stages of hair follicle cycle. Nucleotide composition analysis indicated that guanine (G) and cytosine (C) are the dominant nucleotides in the lncRNA-H19 transcript of Liaoning cashmere goat with the highest frequency distribution (11.25%) of GG nucleotide pair. The regulatory network showed that lncRNA-H19 transcript appears to have remarkably diverse regulatory relationships with its related miRNAs and the potential target genes. In secondary hair follicle, the relative expression of lncRNA-H19 transcript at the anagen phase is significantly higher than that at both telogen and catagen phases suggesting that lncRNA-H19 transcript might play essential roles in the formation and growth of cashmere fiber of goat. Methylation analysis indicated that the methylation of the promoter region of H19 gene most likely participates in its transcriptional suppression in secondary hair follicle of Liaoning cashmere goat.
- Published
- 2017
42. Ubiquitylation of p62/sequestosome1 activates its autophagy receptor function and controls selective autophagy upon ubiquitin stress
- Author
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Peng, H., Yang, J., Li, G., You, Q., Han, W., Li, T., Gao, D., Xie, X., Lee, B.-H., Du, J., Hou, J., Zhang, T., Rao, H., Huang, Y., Li, Q., Zeng, R., Hui, L., Wang, H., Xia, Q., Zhang, X., He, Y., Komatsu, M., Dikic, Y., Finley, D., Hu, R., and Publica
- Abstract
Alterations in cellular ubiquitin (Ub) homeostasis, known as Ub stress, feature and affect cellular responses in multiple conditions, yet the underlying mechanisms are incompletely understood. Here we report that autophagy receptor p62/sequestosome-1 interacts with E2 Ub conjugating enzymes, UBE2D2 and UBE2D3. Endogenous p62 undergoes E2-dependent ubiquitylation during upregulation of Ub homeostasis, a condition termed as Ub+ stress, that is intrinsic to Ub overexpression, heat shock or prolonged proteasomal inhibition by bortezomib, a chemotherapeutic drug. Ubiquitylation of p62 disrupts dimerization of the UBA domain of p62, liberating its ability to recognize polyubiquitylated cargoes for selective autophagy. We further demonstrate that this mechanism might be critical for autophagy activation upon Ub+ stress conditions. Delineation of the mechanism and regulatory roles of p62 in sensing Ub stress and controlling selective autophagy could help to understand and modulate cellular responses to a variety of endogenous and environmental challenges, potentially opening a new avenue for the development of therapeutic strategies against autophagy-related maladies.
- Published
- 2017
43. Environmental behavior of benalaxyl and furalaxyl enantiomers in agricultural soils
- Author
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Peng Xu, Yong X. Gao, Bao Y. Guo, Fang Qin, Jian Z. Li, and Hui L. Wang
- Subjects
China ,Soil ,Soil Pollutants ,Leachate ,Leaching (agriculture) ,Furans ,Alanine ,Chemistry ,Enantioselective synthesis ,Agriculture ,Stereoisomerism ,Sorption ,General Medicine ,Pollution ,Fungicides, Industrial ,Fungicide ,Kinetics ,Biodegradation, Environmental ,Absorption, Physicochemical ,Environmental chemistry ,Soil water ,Degradation (geology) ,Adsorption ,Enantiomer ,Half-Life ,Food Science - Abstract
The enantioselective environmental behavior of the chiral fungicides benalaxy and furalaxyl in agricultural soils in China was studied. Although sorption onto soils was non-enantioselective, the leaching of benalaxy and furalaxyl was enantioselective in soil columns. The concentrations of the S-enantiomers of both fungicides in the leachates were higher than the R-enantiomers. This can be attributed to enantioselective degradation of the two fungicides in the soil column. Enantioselective degradation of the two fungicides was verified by soil dissipation experiments, and the R-enantiomers degraded faster than the S-enantiomers in partial soils. The half-life was 27.7-57.8 days for S-benalaxyl, 20.4-53.3 days for R-benalaxyl, 19.3-49.5 days for S-furalaxyl and 11.4-34.7 days for R-furalaxyl. The degradation process of the two fungicide enantiomers followed the first-order kinetics (R(2)0.96). Compared to furalaxyl, benalaxyl degraded more slowly and degradation was less enantioselective. These results are attributed to the influence of soil physicochemical properties, soil microorganisms, and environmental factors.
- Published
- 2014
44. Identifying species of moths (Lepidoptera) from Baihua Mountain, Beijing, China, using DNA barcodes
- Author
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Robert D. Ward, Hui L. Han, Ai-bing Zhang, Cong H. Yang, and Xiao F. Liu
- Subjects
Species complex ,genetic distance ,Ecology ,diagnostic character ,Cytochrome c oxidase subunit I ,Biodiversity ,Biology ,cytochrome c oxidase subunit I ,DNA barcoding ,Bayesian ,Lepidoptera ,Genetic distance ,Evolutionary biology ,moths ,DNA barcode ,Identification (biology) ,Taxonomic rank ,maximum likelihood ,Neighbor joining ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation ,Original Research ,neighbor joining - Abstract
DNA barcoding has become a promising means for the identification of organisms of all life-history stages. Currently, distance-based and tree-based methods are most widely used to define species boundaries and uncover cryptic species. However, there is no universal threshold of genetic distance values that can be used to distinguish taxonomic groups. Alternatively, DNA barcoding can deploy a “character-based” method, whereby species are identified through the discrete nucleotide substitutions. Our research focuses on the delimitation of moth species using DNA-barcoding methods. We analyzed 393 Lepidopteran specimens belonging to 80 morphologically recognized species with a standard cytochrome c oxidase subunit I (COI) sequencing approach, and deployed tree-based, distance-based, and diagnostic character-based methods to identify the taxa. The tree-based method divided the 393 specimens into 79 taxa (species), and the distance-based method divided them into 84 taxa (species). Although the diagnostic character-based method found only 39 so-identifiable species in the 80 species, with a reduction in sample size the accuracy rate substantially improved. For example, in the Arctiidae subset, all 12 species had diagnostics characteristics. Compared with traditional morphological method, molecular taxonomy performed well. All three methods enable the rapid delimitation of species, although they have different characteristics and different strengths. The tree-based and distance-based methods can be used for accurate species identification and biodiversity studies in large data sets, while the character-based method performs well in small data sets and can also be used as the foundation of species-specific biochips.
- Published
- 2014
45. A critical challenge: Dosage-related efficacy and acute complication intracoronary injection of autologous bone marrow mesenchymal stem cells in acute myocardial infarction
- Author
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Jian L. Shen, Ye Yang, Xue T. Pei, Hui L. Liu, Xue Nan, Hong T. Xu, Yu X. Fei, Da Q. Liu, Feng Guo, Hai T. Tian, Qing A. Ding, Zi C. Tong, Yan H. Shen, Tian C. Li, Yun F. Wang, Li H. Wang, Ning K. Zhang, lian R. Gao, Zhi M. Zhu, Hai Y. Chen, Jian J. Zhang, Yong Yang, Yu Chen, and Zhi G Wang
- Subjects
Male ,Cardiac function curve ,medicine.medical_specialty ,Time Factors ,Myocardial Infarction ,Mesenchymal Stem Cell Transplantation ,Transplantation, Autologous ,law.invention ,stomatognathic system ,Randomized controlled trial ,law ,Internal medicine ,medicine ,Humans ,Single-Blind Method ,Myocardial infarction ,Intraoperative Complications ,Radionuclide Imaging ,Ejection fraction ,Dose-Response Relationship, Drug ,business.industry ,Mesenchymal stem cell ,Middle Aged ,medicine.disease ,Treatment Outcome ,medicine.anatomical_structure ,Injections, Intra-Arterial ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,Complication ,business ,Perfusion ,Artery - Abstract
Background Previous studies showed improvement in heart function by injecting bone marrow mesenchymal stem cells (BMSCs) after AMI. Emerging evidence suggested that both the number and function of BMSCs decline with ageing. We designed a randomized, controlled trial to further investigate the safety and efficacy of this treatment. Methods Patients with ST-elevation AMI undergoing successful reperfusion treatment within 12hours were randomly assigned to receive an intracoronary infusion of BMSCs (n=21) or standard medical treatment (n=22) (the numbers of patients were limited because of the complication of coronary artery obstruction). Results There is a closely positive correlation of the number and function of BMSCs vs. the cardiac function reflected by LVEF at baseline (r=0.679, P=0.001) and at 12-month follow-up (r=0.477, P=0.039). Six months after cell administration, myocardial viability within the infarct area by 18-FDG SPECT was improved in both groups compared with baseline, but no significant difference in the BMSCs compared with control groups (4.0±0.4% 95%CI 3.1–4.9 vs. 3.2±0.5% 95%CI 2.1–4.3, P=0.237). 99mTc-sestamibi SPECT demonstrated that myocardial perfusion within the infarct area in the BMSCs did not differ from the control group (4.4±0.5% 95%CI 3.2–5.5 vs. 3.9±0.6% 95%CI 2.6–5.2, P=0.594). Similarly, LVEF after 12 and 24months follow-up did not show any difference between the two groups. In the BMSCs group, one patient suffered a serious complication of coronary artery occlusion during the BMSCs injection procedure. Conclusions The clinical benefits of intracoronary injection of autologous BMSCs in acute STEMI patients need further investigation and reevaluation.
- Published
- 2013
46. Assessment of drought tolerance of some Triticum L. species through physiological indices
- Author
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Xian, Z.H., Northwest A, Hui, L., Northwest A, Yang, L.J., Northwest A, and Sultan, M.A.R.F., Northwest A
- Subjects
0106 biological sciences ,0301 basic medicine ,Abiotic stress ,fungi ,Drought tolerance ,food and beverages ,Plant Science ,Biology ,Hydroponics ,01 natural sciences ,Crop ,03 medical and health sciences ,030104 developmental biology ,Agronomy ,Genetics ,Cultivar ,Proline ,Common wheat ,Water content ,010606 plant biology & botany - Abstract
Wheat is one of the most important crops in the world. Its yield is greatly influenced by global climate change and scarcity of water in the arid and semi-arid areas of the world. So, exploration of gene resources is of importance to wheat breeding in order to improve the crop ability of coping with abiotic stress environment. Wild relatives of wheat are rich repositories of beneficial genes that confer tolerance or resistance not only to drought but also to other environmental stresses. In the present study, the changes in leaf relative water content (RWC), free proline content, and malondialdehyde (MDA) accumulation of five wild wheat species including T. boeticum (YS-1L), T. dicoccum var. dicoccoides (YS-2L), T. araraticum (ALLT), and two cultivated varieties of T. turgidum ssp. durum (MXLK and 87341), with two well-known common wheat cultivars (SH6 and ZY1) possessing strong drought resistance and sensitiveness, respectively, as references were investigated during 3-day water stress and 2-day recovery , in order to assess the drought tolerance of these wild wheat species. The laboratory experiment was conducted under two water regimes (stress and non-stress treatments). Stress was induced to hydroponically grown two weeks old wheat seedlings with 20% PEG 6000. Stress treatment caused a much smaller decrease in the leaf RWC and rise in MDA content in YS-1L compared to the other wheat species. From the data it was obvious that YS-1L was the most drought tolerant among studied species having signifi - cantly higher proline and RWC while lower MDA content under water stress conditions. The order of water stress tolerance of these species according to the three parameters is: YS-1L > YS-2L > SH6 > 87341 > ZY1 > MXLK > ALLT. We speculate that the observed drought stress tolerance at a cellular level was associated with the ability to accumulate proline and high water level conservation.
- Published
- 2012
47. U74006F REDUCES NEOCORTICAL INFARCTION BUT DOES NOT ATTENUATE SELECTIVE HIPPOCAMPAL CA1 NECROSIS
- Author
-
DONG, X, BRUEDERLIN, B, HEINICKE, E, HUI, L, SLIVKA, A, and BUCHAN, A
- Published
- 2016
48. Recombinant influenza virus with a pandemic H2N2 polymerase complex has a higher adaptive potential than one with seasonal H2N2 polymerase complex
- Author
-
Richard J. Webby, Alex W.H. Chin, Hui-L. Yen, Scott Krauss, and Leo L.M. Poon
- Subjects
0301 basic medicine ,viruses ,Reassortment ,RNA-dependent RNA polymerase ,Biology ,Recombinant virus ,H5N1 genetic structure ,Virus ,Microbiology ,Influenza A Virus, H2N2 Subtype ,03 medical and health sciences ,Viral Proteins ,Viral life cycle ,Virology ,Reassortant Viruses ,Influenza, Human ,Animals ,Humans ,Pandemics ,Polymerase ,Recombination, Genetic ,Influenza A Virus, H3N2 Subtype ,RNA-Dependent RNA Polymerase ,United States ,Standard ,030104 developmental biology ,Mutation ,biology.protein ,Seasons - Abstract
The reassortment of influenza viral gene segments plays a key role in the genesis of pandemic strains. All of the last three pandemic viruses contained reassorted polymerase complexes with subunits derived from animal viruses, suggesting that the acquisition of a reassorted polymerase complex might have a role in generating these pandemic viruses. Here, we studied polymerase activities of the pandemic H2N2, seasonal H2N2 and pandemic H3N2 viruses. We observed that the viral ribonucleoprotein (vRNP) of pandemic H2N2 virus has a highly robust activity. The polymerase activity of seasonal H2N2 viruses, however, was much reduced. We further identified three mutations (PB2-I114V, PB1-S261N and PA-D383N) responsible for the reduced activity. To determine the potential impact of viral polymerase activity on the viral life cycle, recombinant H3N2 viruses carrying pandemic and seasonal H2N2 vRNP were studied in cell cultures supplemented with oseltamivir carboxylate and tested for their abilities to develop adaptive or resistant mutations. It was found that the recombinant virus with pandemic H2N2 vRNP was more capable of restoring the viral fitness than the one with seasonal vRNP. These results suggest that a robust vRNP is advantageous to influenza virus to cope with a new selection pressure.
- Published
- 2016
49. Molecular characterization, expression and methylation status analysis of BMP4 gene in skin tissue of Liaoning cashmere goat during hair follicle cycle
- Author
-
Shu H Yang, Ze Y Wang, Yun L Dang, Hui L. Xue, Wen L. Bai, Yu B. Zhu, Shi Q. Wang, Liang Deng, Rong H. Yin, Jiao J. Wang, Yu Y. Cong, and Dan Guo
- Subjects
0301 basic medicine ,Models, Molecular ,animal structures ,DNA, Complementary ,Transcription, Genetic ,Protein Conformation ,Plant Science ,Bone Morphogenetic Protein 4 ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Complementary DNA ,Genetics ,medicine ,Cashmere goat ,Animals ,Protein Interaction Domains and Motifs ,Amino Acid Sequence ,Cloning, Molecular ,Gene ,Skin ,Messenger RNA ,integumentary system ,Base Sequence ,Goats ,General Medicine ,Methylation ,Sequence Analysis, DNA ,DNA Methylation ,Hair follicle ,Cell biology ,Open reading frame ,030104 developmental biology ,medicine.anatomical_structure ,Bone morphogenetic protein 4 ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Insect Science ,embryonic structures ,Animal Science and Zoology ,CpG Islands ,Hair Follicle - Abstract
Bone morphogenetic protein 4 (BMP4) is a member of the bone morphogenetic protein family (BMPs). It is involved in the development and cycle of hair follicle, as well as, is thought to be a potential candidate gene for cashmere traits in goats. In the present study, we isolated and characterized a full-length open reading frame (ORF) of BMP4 cDNA from the skin tissue of Liaoning cashmere goat, and investigated the transcriptional pattern and methylation status of BMP4 gene in skin tissue of this breed during different stages of hair follicle cycle. The sequence analysis indicated that the isolated cDNA was 1264-bp in length containing a complete ORF of 1230-bp. It encoded a precursor peptide of 409 amino acids with a signal peptide of 19 amino acids. The structural analysis indicated that goat BMP4 contains typical TGF-β propeptide and TGF-β domains. In skin tissue, BMP4 is generally transcribed in an ascendant pattern from anagen to telogen. The methylation level of 5′ flanking regulatory region of BMP4 gene might be involved in its mRNA expression in skin tissue: a higher BMP4 methylation level in skin coincides with a lower expression of BMP4 mRNA. These results from the present work provided a foundation for further insight into the functional and regulatory characteristics of BMP4 in the development and cycle of hair follicle in Liaoning Cashmere goat.
- Published
- 2015
50. Albumin Dialysis Without Anticoagulation in High-Risk Patients: An Observational Study
- Author
-
Kok S. Wong, Wen S. Yang, Han K. Tan, and Hui L. Choong
- Subjects
medicine.medical_specialty ,Bilirubin ,medicine.medical_treatment ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Gastroenterology ,Biomaterials ,chemistry.chemical_compound ,Internal medicine ,medicine ,Coagulopathy ,Saline ,Prothrombin time ,Creatinine ,medicine.diagnostic_test ,business.industry ,Albumin ,General Medicine ,Heparin ,medicine.disease ,Surgery ,chemistry ,business ,medicine.drug ,Partial thromboplastin time - Abstract
Severe liver failure causes coagulopathy and high bleeding risk. Albumin dialysis with Molecular Adsorbent Recirculating System (MARS) (Gambro, Lund, Sweden) is useful for treatment. However, anticoagulation during its use is of uncertain value. We omitted heparin-saline priming and intradialytic heparin and examined its effects. Albumin dialysis was performed in critically ill patients with intermittent circuit saline flushes (2664 ± 2420 mL per treatment). A total of 12 patients (M : F = 10:2; age 49 ± 9 years) were thus treated: 6 for fulminant hepatic failure and 6 for acute-on-chronic liver failure. The overall hospitalization duration was 31 ± 30 days. A total of 44 treatment sessions were performed (average 8 ± 7 sessions per patient). Prescribed versus achieved MARS duration were 13 ± 3 versus 11 ± 4 h, P
- Published
- 2010
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