123 results on '"Hsiang-Chieh Lee"'
Search Results
2. Supplementary Video 4 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Author
-
James L. Connolly, James G. Fujimoto, Aaron D. Aguirre, Tsung-Han Tsai, Amy E. Mondelblatt, Hsiang-Chieh Lee, Yihong Wang, David W. Cohen, and Chao Zhou
- Abstract
Supplementary Video 4 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Published
- 2023
3. Supplementary Figure 1, Legends for Figure 1 and Videos 1-4 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Author
-
James L. Connolly, James G. Fujimoto, Aaron D. Aguirre, Tsung-Han Tsai, Amy E. Mondelblatt, Hsiang-Chieh Lee, Yihong Wang, David W. Cohen, and Chao Zhou
- Abstract
Supplementary Figure 1, Legends for Figure 1 and Videos 1-4 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Published
- 2023
4. Supplementary Video 1 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Author
-
James L. Connolly, James G. Fujimoto, Aaron D. Aguirre, Tsung-Han Tsai, Amy E. Mondelblatt, Hsiang-Chieh Lee, Yihong Wang, David W. Cohen, and Chao Zhou
- Abstract
Supplementary Video 1 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Published
- 2023
5. Supplementary Video 2 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Author
-
James L. Connolly, James G. Fujimoto, Aaron D. Aguirre, Tsung-Han Tsai, Amy E. Mondelblatt, Hsiang-Chieh Lee, Yihong Wang, David W. Cohen, and Chao Zhou
- Abstract
Supplementary Video 2 from Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
- Published
- 2023
6. Early detection of caries with ultrahigh-resolution optical coherence tomography (Conference Presentation)
- Author
-
Tai-Ang Wang, Hsiang-Chieh Lee, Yen-Li Wang, and Meng-Tsan Tsai
- Published
- 2023
7. Polarization-sensitive imaging using optical coherence tomography and a HCG-VCSEL laser
- Author
-
Chien-Hua Peng, Yu-Cheng Mei, Hung-Kai Chen, Ting-Yen Tsai, Ting-Hao Chen, Chuan-Bor Chueh, Dalila Ellafi, Chris Chase, Hao-Chung Kuo, Michael C. Y. Huang, and Hsiang-Chieh Lee
- Published
- 2023
8. Development of an automatic algorithm enabling layer segmentation and optical characteristic analysis in skin optical coherence tomography imaging
- Author
-
Ting-Hao Chen, Yu-Hsuan Lee, Chau Yee Ng, Meng-Tsan Tsai, Cheng-Kuang Lee, and Hsiang-Chieh Lee
- Published
- 2023
9. Continuous blood pressure monitoring from an autonomic nervous system perspective
- Author
-
Ting-Yu Chiang, Shih-Lun Tai, Yu-Ting Liu, Jiun-Woei Huang, Shu-Sheng Lee, Hsiang-Chieh Lee, and Chih-Kung Lee
- Published
- 2023
10. C-Band Silicon Waveguide Modulation With 50-Gbit/s NRZ-OOK Over 10-km SMF and DSF
- Author
-
Gong-Ru Lin, Hsiang-Chieh Lee, Shih-Chun Kao, Patrick Chiang, Bo-Yuan Lee, Huang-Shen Lin, Cheng-Ting Tsai, and Chih-Hsien Cheng
- Subjects
Physics ,Silicon ,business.industry ,C band ,Electro-optic modulator ,chemistry.chemical_element ,Condensed Matter Physics ,Waveguide (optics) ,Atomic and Molecular Physics, and Optics ,Amplitude-shift keying ,chemistry ,Transmission (telecommunications) ,Modulation ,Optoelectronics ,Electrical and Electronic Engineering ,business ,Phase shift module - Abstract
A low-power-consumption single-arm-driven silicon (Si) Mach-Zehnder modulator (MZM) operated at C-band ( $\lambda =1550$ nm) for non-return-to-zero on-off-keying (NRZ-OOK) transmission beyond 50 Gbit/s over 10-km single-mode fiber (SMF) and dispersion-shift fiber (DSF) is demonstrated to guarantee the IEEE 802.3bs standardized 400GBASE-FR8/LR8 operation. The Si MZM exhibits asymmetric waveguide arms and 2-mm-contact-length phase shifter under carrier depletion operation in its p-n junction, which is DC biased as low as 2 V to provide a low $\text{V}_{\pi }\text{L}$ product of $0.909~\text {V}\cdot \text {cm}$ . For the error-free transmission of the NRZ-OOK data-stream, the single-arm-driven Si MZM supports the highest data rates of 53, 53 and 50 Gbit/s over optical back-to-back, 2-km SMF and 10-km DSF transmissions, respectively. The 50-Gbit/s receiving power penalties are respectively 0.2 and 0.25 dB after 2-km SMF and 10-km DSF propagation.
- Published
- 2021
11. Estimating the elasticity properties of arterial phantoms using fiber-based laser doppler vibrometry
- Author
-
Yu-Ting Liu, Shu-Sheng Lee, Hsiang-Chieh Lee, and Chih-Kung Lee
- Published
- 2022
12. Effect of A-scan rate and interscan interval on optical coherence angiography
- Author
-
Yin-Peng Huang, Yi-Chun Wu, Yoshiaki Yasuno, Ting-Yen Tsai, Chuan-Bor Chueh, Ting-Hao Chen, Bo-Huei Huang, Meng-Tsan Tsai, and Hsiang-Chieh Lee
- Subjects
Horizontal scan rate ,0303 health sciences ,Noninvasive imaging ,Materials science ,medicine.diagnostic_test ,Image processing ,Iterative reconstruction ,01 natural sciences ,Article ,Atomic and Molecular Physics, and Optics ,010309 optics ,03 medical and health sciences ,Optical coherence tomography ,0103 physical sciences ,Angiography ,otorhinolaryngologic diseases ,medicine ,sense organs ,Optical tomography ,030304 developmental biology ,Biotechnology ,Coherence (physics) ,Biomedical engineering - Abstract
Optical coherence tomography angiography (OCTA) can provide rapid, volumetric, and noninvasive imaging of tissue microvasculature without the requirement of exogenous contrast agents. To investigate how A-scan rate and interscan time affected the contrast and dynamic range of OCTA, we developed a 1.06-µm swept-source OCT system enabling 100-kHz or 200-kHz OCT using two light sources. After system settings were carefully adjusted, almost the same detection sensitivity was achieved between the 100-kHz and 200-kHz modalities. OCTA of ear skin was performed on five mice. We used the variable interscan time analysis algorithm (VISTA) and the designated scanning protocol with OCTA images reconstructed through the correlation mapping method. With a relatively long interscan time (e.g., 12.5 ms vs. 6.25 ms for 200-kHz OCT), OCTA can identify more intricate microvascular networks. OCTA image sets with the same interscan time (e.g., 12.5 ms) were compared. OCTA images acquired with a 100-kHz A-scan rate showed finer microvasculature than did other imaging modalities. We performed quantitative analysis on the contrast from OCTA images reconstructed with different A-scan rates and interscan time intervals in terms of vessel area, total vessel length, and junction density.
- Published
- 2021
13. Quantitative comparison of photothermal effects induced by pulsed lasers with optical coherence tomography
- Author
-
Tai-Ang Wang, Hsiang-Chieh Lee, Chau Yee Ng, and Meng-Tsan Tsai
- Published
- 2022
14. High-speed optical coherence tomography imaging with a tunable HCG-VCSEL light source at the 1060nm wavelength window
- Author
-
Ting-Yen Tsai, Ting-Hao Chen, Hung-Kai Chen, Chuan-Bor Chueh, Dalila Ellafi, Chris Chase, Hao-Chung Kuo, Meng-Tsan Tsai, Michael C. Y. Huang, and Hsiang-Chieh Lee
- Published
- 2022
15. In Vivo Identification of Skin Photodamage Induced by Fractional CO
- Author
-
Chau Yee, Ng, Tai-Ang, Wang, Hsiang-Chieh, Lee, Bo-Huei, Huang, and Meng-Tsan, Tsai
- Abstract
Fractional laser treatment is commonly used for dermatological applications, enabling effective induction of collagen regeneration and significantly reducing recovery time. However, it is challenging to observe laser-induced photodamage beneath the tissue surface in vivo, making the non-invasive evaluation of treatment outcomes difficult. For in vivo real-time study of the photodamage induced by fractional pulsed CO
- Published
- 2022
16. Optical Coherence Tomography/Angiography-Guided Tumor Ablation With a Continuous-Wave Laser Diode
- Author
-
Meng-Tsan Tsai, Y. J. Lee, Chun Chieh Wang, Hsiang-Chieh Lee, Wen Ju Chen, and Ting Yen Tsai
- Subjects
Materials science ,General Computer Science ,medicine.medical_treatment ,biomedical optical imaging ,law.invention ,Optical coherence tomography ,law ,medicine ,General Materials Science ,angiography ,Diode ,Laser ablation ,medicine.diagnostic_test ,Laser diode ,General Engineering ,biophotonics ,medical diagnostic imaging ,Photothermal therapy ,Ablation ,Biophotonics ,laser ablation ,Continuous wave ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,lcsh:TK1-9971 ,Biomedical engineering - Abstract
Laser ablation can be an effective modality for treatment, but it is complicated to apply continuous-wave (CW) light sources for laser ablation because of the unpredictable photothermal damage. In this study, an integrated theranostic system combining a low-cost CW laser diode with optical coherence tomography (OCT)/angiography (OCTA) was utilized for the in vivo ablation of tumor tissues. To examine the effect of laser exposure on tissue scattering characteristics, the OCT backscattering intensities of non-ablated and ablated tissues were analyzed, and the effect on the skin microvasculature produced by laser ablation was quantitatively evaluated. Moreover, the integrated system and the proposed method were implemented for the treatment of skin tumor on the mouse model. The obtained results indicate that the developed laser ablation system can effectively remove tumor tissues with controllable photodamage under OCT/OCTA guidance and that the system cost may be significantly reduced by using the CW laser diode.
- Published
- 2020
17. A Generic Framework for Fourier-Domain Optical Coherence Tomography Imaging: Software Architecture and Hardware Implementations
- Author
-
Yi-Chung Wu, Hung-Wen Chen, Hsiang-Chieh Lee, Ting-Hao Chen, Yu-Wei Chang, Yin-Peng Huang, Ting-Yen Tsai, You-Nan Tsai, Chuan-Bor Chueh, Yi-Ping Hung, and Meng-Tsan Tsai
- Subjects
General Computer Science ,genetic structures ,parallel processing ,Computer science ,data acquisition ,Image processing ,Iterative reconstruction ,01 natural sciences ,010309 optics ,03 medical and health sciences ,Data acquisition ,Software ,Optical coherence tomography ,Robustness (computer science) ,0103 physical sciences ,medicine ,Waveform ,General Materials Science ,030304 developmental biology ,software architecture ,0303 health sciences ,Signal processing ,medicine.diagnostic_test ,business.industry ,General Engineering ,biophotonics ,Schematic ,medical diagnostic imaging ,eye diseases ,Biophotonics ,sense organs ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,Software architecture ,business ,Biomedical optical imaging ,lcsh:TK1-9971 ,Computer hardware - Abstract
Fourier-domain optical coherence tomography (FD-OCT), including spectral-domain OCT (SD-OCT) and swept-source OCT (SS-OCT), allows the volumetric imaging of the tissue architecture with a faster speed and higher detection sensitivity than does time-domain OCT. Although the hardware implementations of SD-OCT and SS-OCT are different, these technologies share very similar signal processing steps for image reconstruction. In this study, we developed hardware implementations and software architectures to design a generic framework for FD-OCT. For SD-OCT systems, an external synchronization approach was used to realize a data acquisition schematic similar to that used in SS-OCT by carefully managing the timing clocks in the detection unit and for the waveform generation. In addition, by utilizing modules and factory concepts, a software engine can be developed that supports various acquisition devices and software operations or image processing functions with high operation flexibility while maintaining its robustness. Data processing and data saving were optimized using the parallel computing method with the OpenMP library and by leveraging the parallelism within the acquired data, respectively.
- Published
- 2020
18. Upregulated NPM1 is an independent biomarker to predict progression and prognosis of oral squamous cell carcinomas in Taiwan
- Author
-
Mark Yen-Ping Kuo, Pei-Yao Pan, Yi Ping Wang, Hsin-Hui Peng, Hsiang-Chieh Lee, Nai‐Chi Chi, Shih-Jung Cheng, and Hui-Hsin Ko
- Subjects
0301 basic medicine ,Epithelial dysplasia ,NPM1 ,medicine.medical_treatment ,Cell ,Taiwan ,Targeted therapy ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Transcription (biology) ,Biomarkers, Tumor ,medicine ,Humans ,Nucleoplasmins ,Nucleophosmin ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Mouth Mucosa ,Nuclear Proteins ,Prognosis ,stomatognathic diseases ,030104 developmental biology ,medicine.anatomical_structure ,Otorhinolaryngology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Disease Progression ,Cancer research ,Immunohistochemistry ,Mouth Neoplasms ,business - Abstract
Background Nucleophosmin/nucleoplasmin family 1 (NPM1) has broad physiological functions, such as DNA replication, transcription, ribosome biogenesis, and centrosome replication. This study explored the clinicopathological importance of NPM1 as a prognostic marker for oral squamous cell carcinoma (OSCC). Methods We collected specimens from 96 OSCC, 45 oral epithelial dysplasia (OED), and 29 normal oral mucosa (NOM). NPM1 expression was analyzed via immunohistochemistry. Correlations between NPM1and clinical parameters were analyzed using Student t test, chi-squared test, and Kaplan-Meier product-limit method. Results The NPM1 labeling indices (LIs) were significantly higher in OSCCs than in NOM and oral OED. Higher NPM1 expression was significantly correlated with larger tumor size, nodal metastasis, and advanced clinical stage. Multivariate analysis revealed that higher NPM1 LIs were an unfavorable independent factor for survival. Conclusions Upregulated NPM1 is an independent biomarker of poor prognosis and NPM1 inhibitors may be promising in molecular targeted therapy against OSCC.
- Published
- 2019
19. Identification of changes in the microvasculature in mouse brain among different physiological states using optical coherence tomography angiography
- Author
-
Ting-Hao Chen, Yi-Chun Wu, Chih-Chang Li, Ming-An Chen, Ting-Yen Tsai, Chuan-Bor Chueh, Meng-Tsan Tsai, Yoshiaki Yasuno, Ming-Kai Pan, and Hsiang-Chieh Lee
- Published
- 2021
20. Graphics Processing Unit (GPU) accelerated microvascular imaging framework with optical coherence tomography (OCT) and OCT angiography techniques
- Author
-
Meng-Tsan Tsai, Cheng-Kuang Lee, Ting-Hao Chen, Hsiang-Chieh Lee, Yi-Chun Wu, Ting-Yen Tsai, Chuan-Bor Chueh, and Yu-Ling Chen
- Subjects
genetic structures ,medicine.diagnostic_test ,Computer science ,Graphics processing unit ,Image processing ,Signal ,eye diseases ,Visualization ,Oct angiography ,Data acquisition ,Optical coherence tomography ,Angiography ,medicine ,sense organs ,Biomedical engineering - Abstract
Recently, the functional extension of optical coherence tomography (OCT) with OCT angiography (OCTA) allows volumetric imaging of the subsurface microvasculature without requiring exogenous contrast agents like conventional angiography techniques. However, performing OCTA requires intensive computation to extract the changes of OCT signal due to moving red blood cells in the microvascular network. In this study, we have developed a graphic processing unit (GPU)-accelerated framework to realize high speed OCTA imaging and the visualization of the microvascular network after the data acquisition. In addition, we investigate the feasibility of providing real-time microvascular imaging leveraging dynamic scattering OCT and GPU.
- Published
- 2021
21. High-speed and wide-field endoscopic optical coherence tomography imaging of the oral mucosa with a micromotor imaging catheter and polarization diversity detection
- Author
-
Ting-Yen Tsai, Chuan-Bor Chueh, Meng-Shan Wu, Meng-Tsan Tsai, Ting-Hao Chen, and Hsiang-Chieh Lee
- Subjects
Catheter ,Materials science ,medicine.anatomical_structure ,Optical coherence tomography ,medicine.diagnostic_test ,Micromotor ,medicine ,Oral mucosa ,Wide field ,Polarization diversity ,Biomedical engineering - Published
- 2021
22. Development of a miniature imaging head combing wide-angle camera and optical coherence tomography for the semiautonomous laparoscope surgery procedure
- Author
-
Cheng-Wei Chen, Meng-Shan Wu, Hsiang-Chieh Lee, Zheng-Jie Wu, and Ting-Yen Tsai
- Subjects
Optical coherence tomography ,medicine.diagnostic_test ,Surgery procedure ,Computer science ,medicine ,Head (vessel) ,Combing ,Biomedical engineering - Published
- 2021
23. Assessment of chronic radiation proctopathy and radiofrequency ablation treatment follow-up with optical coherence tomography angiography: A pilot study
- Author
-
Kaicheng Liang, James G. Fujimoto, Hiroshi Mashimo, Zhao Wang, Osman O. Ahsen, and Hsiang-Chieh Lee
- Subjects
Male ,medicine.medical_specialty ,Chronic radiation proctopathy ,Radiofrequency ablation ,Colonoscopy ,Rectum ,Observational Study ,Pilot Projects ,Proctoscopy ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,Submucosa ,Ectasia ,medicine ,Humans ,Subsurface microvascular imaging ,Telangiectasia ,Rectal telangiectasia density scoring system ,Radiation Injuries ,Aged ,Aged, 80 and over ,Radiofrequency Ablation ,medicine.diagnostic_test ,Optical coherence tomography ,business.industry ,Gastroenterology ,Angiography ,General Medicine ,Optical coherence tomography angiography ,3. Good health ,Endoscopy ,Catheter ,medicine.anatomical_structure ,Rectal Diseases ,Treatment Outcome ,030220 oncology & carcinogenesis ,Chronic Disease ,Microvessels ,Quality of Life ,Feasibility Studies ,030211 gastroenterology & hepatology ,Radiology ,medicine.symptom ,business ,Tomography, Optical Coherence ,Follow-Up Studies - Abstract
BACKGROUND Chronic radiation proctopathy (CRP) occurs as a result of pelvic radiation therapy and is associated with formation of abnormal vasculature that may lead to persistent rectal bleeding. While incidence is declining due to refinement of radiation delivery techniques, CRP remains one of the major complications of pelvic radiation therapy and significantly affects patient quality of life. Radiofrequency ablation (RFA) is an emerging treatment modality for eradicating abnormal vasculature associated with CRP. However, questions remain regarding CRP pathophysiology and optimal disease management. AIM To study feasibility of optical coherence tomography angiography (OCTA) for investigating subsurface vascular alterations in CRP and response to RFA treatment. METHODS Two patients with normal rectum and 8 patients referred for, or undergoing endoscopic RFA treatment for CRP were imaged with a prototype ultrahigh-speed optical coherence tomography (OCT) system over 15 OCT/colonoscopy visits (2 normal patients, 5 RFA-naïve patients, 8 RFA-follow-up visits). OCT and OCTA was performed by placing the OCT catheter onto the dentate line and rectum without endoscopic guidance. OCTA enabled depth-resolved microvasculature imaging using motion contrast from flowing blood, without requiring injected dyes. OCTA features of normal and abnormal microvasculature were assessed in the mucosa and submucosa. Blinded reading of OCTA images was performed to assess the association of abnormal rectal microvasculature with CRP and RFA treatment, and rectal telangiectasia density endoscopic scoring. RESULTS OCTA/OCT images are intrinsically co-registered and enabled depth-resolved visualization of microvasculature in the mucosa and submucosa. OCTA visualized normal vascular patterns with regular honeycomb patterns vs abnormal vasculature with distorted honeycomb patterns and ectatic/tortuous microvasculature in the rectal mucosa. Normal arterioles and venules < 200 μm in diameter versus abnormal heterogenous enlarged arterioles and venules > 200 μm in diameter were visualized in the rectal submucosa. Abnormal mucosal vasculature occurred in 0 of 2 normal patients and 3 of 5 RFA-naïve patients, while abnormal submucosal vasculature occurred more often, in 1 of 2 normal patients and 5 of 5 RFA-naïve patients. After RFA treatment, vascular abnormalities decreased, with abnormal mucosal vasculature observed in 0 of 8 RFA-follow-up visits and abnormal submucosal vasculature observed in only and 2 of 8 RFA-follow-up visits. CONCLUSION OCTA visualizes depth-resolved microvascular abnormalities in CRP, allowing assessment of superficial features which are endoscopically visible as well as deeper vasculature which cannot be seen endoscopically. OCTA/OCT of the rectum can be performed in conjunction with, or independently from endoscopy. Further studies are warranted to investigate if OCTA/OCT can elucidate pathophysiology of CRP or improve management.
- Published
- 2019
24. Trust in Government, Social Determinants, and Resource Distribution after a Catastrophic Typhoon
- Author
-
Chuan-Zhong Deng, Hsiang-Chieh Lee, and Hongey Chen
- Subjects
Government ,Economic growth ,Typhoon ,Social impact ,Ethnic group ,Resource distribution ,General Social Sciences ,Building and Construction ,Business ,Social determinants of health ,General Environmental Science ,Civil and Structural Engineering - Abstract
This study investigated the effects of social determinants and resource distribution on trust in government after a disaster. This involved an analysis of four waves of the Social Impact an...
- Published
- 2021
25. β-glucan therapy converts the inhibition of myeloid-derived suppressor cells in oral cancer patients
- Author
-
Ya-Wen Lo, Alan Yueh-Luen Lee, Hsiang-Chieh Lee, Shih-Jung Cheng, Hui-Hsin Ko, Chun-Pin Chiang, Hsin-Hui Peng, Yi-Ching Liu, and Pei-Yao Pan
- Subjects
Oncology ,medicine.medical_specialty ,beta-Glucans ,CD33 ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,Internal medicine ,medicine ,Humans ,General Dentistry ,Glucan ,chemistry.chemical_classification ,medicine.diagnostic_test ,biology ,Proportional hazards model ,business.industry ,Squamous Cell Carcinoma of Head and Neck ,Myeloid-Derived Suppressor Cells ,Cancer ,030206 dentistry ,medicine.disease ,stomatognathic diseases ,Otorhinolaryngology ,Integrin alpha M ,chemistry ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Myeloid-derived Suppressor Cell ,biology.protein ,Carcinoma, Squamous Cell ,Mouth Neoplasms ,business - Abstract
OBJECTIVES The myeloid-derived suppressor cells (MDSCs) frequently have a high expansion in cancer patients. This research explored whether administration of β-glucan could increase anti-tumor immunity in oral squamous cell carcinoma (OSCC) patients. MATERIALS AND METHODS This study evaluated the MDSC level of circulating blood as CD33+ /CD11b+ /HLA-DR-/low by flow cytometry in 30 healthy donors (HDs, group I), in 48 oral squamous cell carcinoma (OSCC) patients before and after 14-day preoperative administration of β-glucan (group II), and in 52 OSCC patients without taking β-glucan (group III). RESULTS A significantly higher mean MDSC level was observed in 100 OSCC patients than in 30 HDs (p
- Published
- 2021
26. Quantitative spectroscopic comparison of the optical properties of mouse cochlea microstructures using optical coherence tomography at 1.06 µm and 1.3 µm wavelengths
- Author
-
Hsin-Chien Chen, Meng-Tsan Tsai, Chih-Hung Wang, Bernhard Baumann, Hsiang-Chieh Lee, Ting-Hao Chen, Yi-Ping Hung, Ting-Yen Tsai, Chuan-Bor Chueh, and Yin-Peng Huang
- Subjects
Materials science ,medicine.medical_treatment ,Image processing ,01 natural sciences ,Article ,law.invention ,010309 optics ,03 medical and health sciences ,0302 clinical medicine ,Optical coherence tomography ,law ,Cochlear implant ,0103 physical sciences ,medicine ,otorhinolaryngologic diseases ,Optical tomography ,030223 otorhinolaryngology ,Cochlea ,medicine.diagnostic_test ,Atomic and Molecular Physics, and Optics ,Lens (optics) ,Wavelength ,Tomography ,sense organs ,Biotechnology ,Biomedical engineering - Abstract
Currently, the cochlear implantation procedure mainly relies on using a hand lens or surgical microscope, where the success rate and surgery time strongly depend on the surgeon’s experience. Therefore, a real-time image guidance tool may facilitate the implantation procedure. In this study, we performed a systematic and quantitative analysis on the optical characterization of ex vivo mouse cochlear samples using two swept-source optical coherence tomography (OCT) systems operating at the 1.06-µm and 1.3-µm wavelengths. The analysis results demonstrated that the 1.06-µm OCT imaging system performed better than the 1.3-µm OCT imaging system in terms of the image contrast between the cochlear conduits and the neighboring cochlear bony wall structure. However, the 1.3-µm OCT imaging system allowed for greater imaging depth of the cochlear samples because of decreased tissue scattering. In addition, we have investigated the feasibility of identifying the electrode of the cochlear implant within the ex vivo cochlear sample with the 1.06-µm OCT imaging. The study results demonstrated the potential of developing an image guidance tool for the cochlea implantation procedure as well as other otorhinolaryngology applications.
- Published
- 2021
27. In Vivo Identification of Skin Photodamage Induced by Fractional CO2 and Picosecond Nd:YAG Lasers with Optical Coherence Tomography
- Author
-
Chau Yee Ng, Tai-Ang Wang, Hsiang-Chieh Lee, Bo-Huei Huang, and Meng-Tsan Tsai
- Subjects
Clinical Biochemistry ,photodamage ,therapy ,optical coherence tomography ,skin ,diagnosis ,sense organs - Abstract
Fractional laser treatment is commonly used for dermatological applications, enabling effective induction of collagen regeneration and significantly reducing recovery time. However, it is challenging to observe laser-induced photodamage beneath the tissue surface in vivo, making the non-invasive evaluation of treatment outcomes difficult. For in vivo real-time study of the photodamage induced by fractional pulsed CO2 and Nd:YAG lasers commonly utilized for clinical therapy, a portable spectral-domain optical coherence tomography (SD-OCT) system was implemented for clinical studies. The photodamage caused by two lasers, including photothermal and photoacoustic effects, was investigated using OCT, together with the correlation between photodamage and exposure energy. Additionally, to investigate the change in the optical properties of tissue due to photodamage, the attenuation coefficients and damaged areas of normal skin and laser-treated skin were estimated for comparison. Finally, the recovery of the exposed skin with both lasers was also compared using OCT. The results show that OCT can be a potential solution for in vivo investigation of laser-induced tissue damage and quantitative evaluation.
- Published
- 2022
28. Metasurface‐Based Abrupt Autofocusing Beam for Biomedical Applications
- Author
-
Yuan Luo, Ming Lun Tseng, Sunil Vyas, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Hsiang‐Chieh Lee, Wen‐Pin Chen, Vin‐Cent Su, Xu Shi, Hiroaki Misawa, Din Ping Tsai, and Pan‐Chyr Yang
- Subjects
Mice ,Optics and Photonics ,Swine ,Lasers ,Animals ,General Materials Science ,General Chemistry - Abstract
Manipulation and precise delivery of optical energies in the regions of interest within specimens require different strategies. Hence, proper control of input beam parameters is a prerequisite. One of the prominent methods is metasurface optics, capable of crafting properties of light at nanoscales. Here, the generation of an abrupt autofocusing (AAF) beam by a nanophotonic metasurface for biomedical applications is demonstrated. Fluorescence guided laser microprofiling of mouse cardiac samples is experimentally investigated, using the AAF beam to deliver optical energy selectively to specific locations. In addition, photocoagulation of ex vivo swine skin tissue is performed and observed through optical coherence tomography. The results show great potentials for integrating metasurface optics to realize miniature laser surgery instruments for wide applications in biomedicine.
- Published
- 2022
29. Real-time functional optical coherence tomography imaging
- Author
-
Meng-Tsan Tsai, Hsiang-Chieh Lee, Yin-Peng Huang, Ting-Hao Chen, Cheng-Kuang Lee, Ting-Yen Tsai, Chuan-Bor Chueh, and Teng-Chieh Chang
- Subjects
Physics ,Optics ,Optical coherence tomography ,medicine.diagnostic_test ,business.industry ,medicine ,business - Published
- 2020
30. Multiscale imaging of the ex vivo oral precancerous lesions with a custom developed swept-source optical coherence tomography/microscopy platform and a 1.7 µm wavelength-swept laser (Conference Presentation)
- Author
-
Ching-Yu Wang, Ting-Hao Chen, You-Nan Tsai, Yin-Peng Huang, Hsiang-Chieh Lee, Teng-Chieh Chang, Yi-Ping Hung, Ting-Yen Tsai, and Chuan-Bor Chueh
- Subjects
genetic structures ,medicine.diagnostic_test ,business.industry ,Late stage ,Early detection ,Control software ,Laser ,eye diseases ,law.invention ,stomatognathic diseases ,Optical coherence tomography ,law ,Microscopy ,Medicine ,sense organs ,business ,Nuclear medicine ,Ex vivo - Abstract
Oral cancer was ranked as the fifth most common cancers in both sexes in Taiwan in 2014. For patients diagnosed with the advanced or late stage of oral SCC, the five-year survival rate is reported to be ~ 33% suggesting the importance of the early detection of oral cancer. There have been various studies of investigating the clinical utility of OCT for the early detection of oral precancerous lesions with 1300 nm OCT technology. In this study, we have developed a long-wavelength, multiscale OCT imaging system enabling multiscale imaging of the ex vivo oral precancerous tissue with an increased imaging depth. Objectives with two different magnifications are mounted to a power turret, enabling seamless change of the OCT imaging resolution via the software control. OCT imaging over a variety of oral precancer pathologies will be demonstrated with above OCT system.
- Published
- 2020
31. Development of a time lapse tumor cell spheroid imaging system with a high-resolution spectral-domain optical coherence microscopy system (Conference Presentation)
- Author
-
Ting-Hao Chen, You-Nan Tsai, Chih-Chung Yang, Ting-Yen Tsai, Yu-Wei Chang, Chia-Chun Ni, Hsiang-Chieh Lee, and Ying-Peng Huang
- Subjects
Physics ,Optics ,business.industry ,Resolution (electron density) ,Optical coherence microscopy ,Spheroid ,Spectral domain ,Tumor cells ,business - Published
- 2020
32. Quantitative spectroscopic comparison of the optical properties of the mouse cochlear microstructures using optical coherence tomography at 1.3 µm and 1 µm wavelength regimes (Conference Presentation)
- Author
-
Meng-Tsan Tsai, Yin-Peng Huang, Hao Wang, Chih-Hung Wang, Hsiang-Chieh Lee, Ting Hao Chen, Ting-Yen Tsai, Yi-Ping Hung, Hsin-Chien Chen, Teng-Chieh Chang, and Yu-Wei Chang
- Subjects
Wavelength ,Materials science ,Optics ,Optical coherence tomography ,medicine.diagnostic_test ,business.industry ,medicine ,Microstructure ,business - Published
- 2020
33. Assessment of Barrett’s esophagus and dysplasia with ultrahigh-speed volumetric en face and cross-sectional optical coherence tomography
- Author
-
Michael G. Giacomelli, Benjamin Potsaid, Marisa Figueiredo, Qin Huang, Hiroshi Mashimo, Zhao Wang, James G. Fujimoto, Kaicheng Liang, Osman O. Ahsen, Hsiang-Chieh Lee, and Vijaysekhar Jayaraman
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Esophageal Mucosa ,Esophageal Neoplasms ,Biopsy ,Endoscopic mucosal resection ,Article ,Barrett Esophagus ,03 medical and health sciences ,0302 clinical medicine ,Optical coherence tomography ,medicine ,Humans ,Esophagus ,Treatment history ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,Reproducibility of Results ,Histology ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,Dysplasia ,030220 oncology & carcinogenesis ,Barrett's esophagus ,Female ,030211 gastroenterology & hepatology ,Esophagoscopy ,business ,Precancerous Conditions ,Tomography, Optical Coherence - Abstract
This study aimed to evaluate the use of ultrahigh-speed volumetric en face and cross-sectional optical coherence tomography (OCT) with micromotor catheters for the in vivo assessment of Barrett's esophagus and dysplasia.74 OCT datasets with correlated biopsy/endoscopic mucosal resection histology (49 nondysplastic Barrett's esophagus [NDBE], 25 neoplasia) were obtained from 14 patients with Barrett's esophagus and a history of dysplasia and 30 with NDBE. The associations between irregular mucosal patterns on en face OCT, absence of mucosal layering, surface signal subsurface, and 5 atypical glands on cross-sectional OCT vs. histology and treatment history were assessed by three blinded readers.Atypical glands under irregular mucosal patterns occurred in 75 % of neoplasia (96 % of treatment-naïve neoplasia) vs. 30 % of NDBE datasets (43 % of short- and 18 % of long-segment NDBE). Mucosal layering was absent in 35 % of neoplasia and 50 % of NDBE datasets, and surface signal subsurface occurred in 29 % of neoplasia and 30 % of NDBE datasets.Atypical glands under irregular mucosal patterns are strongly associated with neoplasia, suggesting potential markers for dysplasia and a role in pathogenesis.This study aimed to evaluate the use of ultrahigh-speed volumetric en face and cross-sectional optical coherence tomography (OCT) with micromotor catheters for the in vivo assessment of Barrett’s esophagus and dysplasia.74 OCT datasets with correlated biopsy/endoscopic mucosal resection histology (49 nondysplastic Barrett’s esophagus [NDBE], 25 neoplasia) were obtained from 14 patients with Barrett’s esophagus and a history of dysplasia and 30 with NDBE. The associations between irregular mucosal patterns on en face OCT, absence of mucosal layering, surface signal subsurface, and 5 atypical glands on cross-sectional OCT vs. histology and treatment history were assessed by three blinded readers.Atypical glands under irregular mucosal patterns occurred in 75 % of neoplasia (96 % of treatment-naïve neoplasia) vs. 30 % of NDBE datasets (43 % of short- and 18 % of long-segment NDBE). Mucosal layering was absent in 35 % of neoplasia and 50 % of NDBE datasets, and surface signal subsurface occurred in 29 % of neoplasia and 30 % of NDBE datasets.Atypical glands under irregular mucosal patterns are strongly associated with neoplasia, suggesting potential markers for dysplasia and a role in pathogenesis.
- Published
- 2018
34. Ultrahigh-resolution optical coherence tomography/angiography with an economic and compact supercontinuum laser
- Author
-
Cheng Yu Lee, Ming Che Chan, Meng-Tsan Tsai, Tai Ang Wang, and Hsiang-Chieh Lee
- Subjects
0303 health sciences ,Materials science ,Spectrometer ,medicine.diagnostic_test ,business.industry ,Relative intensity noise ,01 natural sciences ,Noise (electronics) ,Atomic and Molecular Physics, and Optics ,Article ,Supercontinuum ,010309 optics ,03 medical and health sciences ,symbols.namesake ,Optics ,Optical coherence tomography ,0103 physical sciences ,symbols ,medicine ,business ,Adaptive optics ,Doppler effect ,Preclinical imaging ,030304 developmental biology ,Biotechnology - Abstract
In this study, a Q-switch pumped supercontinuum laser (QS-SCL) is used as a light source for in vivo imaging via ultrahigh-resolution optical coherence tomography and angiography (UHR-OCT/OCTA). For this purpose, an OCT system based on a spectral-domain detection scheme is constructed, and a spectrometer with a spectral range of 635 − 875 nm is designed. The effective full-width at half maximum of spectrum covers 150 nm, and the corresponding axial and transverse resolutions are 2 and 10 µm in air, respectively. The relative intensity noise of the QS-SCL and mode-locked SCL is quantitatively compared. Furthermore, a special processing algorithm is developed to eliminate the intrinsic noise of QS-SCL. This work demonstrates that QS-SCLs can effectively reduce the cost and size of UHR-OCT/OCTA instruments, making clinical applications feasible.
- Published
- 2019
35. Social determinants in choice of shelter: an evidence-based analysis
- Author
-
Hsiang-Chieh Lee and Hongey Chen
- Subjects
021110 strategic, defence & security studies ,Atmospheric Science ,Government ,Social network ,business.industry ,05 social sciences ,Social change ,0211 other engineering and technologies ,02 engineering and technology ,Renting ,0502 economics and business ,Earth and Planetary Sciences (miscellaneous) ,Survey data collection ,Social determinants of health ,business ,Psychology ,Natural disaster ,Socioeconomics ,050203 business & management ,Water Science and Technology ,Social capital - Abstract
This research looked for social determinants that shaped choices of shelter during a natural disaster. The choices consisted of hotels/rental houses, finding relatives/friends, contacting the government for help, and checking for information about public shelters online. Social determinants examined included age, disability, education, income, social network, trust in the government, and previous disaster experience. The 2013 Taiwan Social Change Survey data and logistic regression were used for analysis. The findings were as follows: (1) compared with other groups, the richest favored hotels or rental houses for sheltering. (2) Compared with other groups, people with lower levels of education had a tendency to contact the government for help regarding shelter. (3) Compared with other groups, young people (20–34 years old), people with larger social networks (informal social capital), and people with more than one previous disaster experience preferred to contact relatives or friends for shelter. (4) People with at least a senior high school education were more likely to search for sheltering information online than their counterparts. This study provides new contributions to the literature in its investigation of the influences of social networks and previous natural disaster experience on shelter choice and of education on the choice of checking sheltering information online.
- Published
- 2018
36. Ultrahigh-speed endoscopic optical coherence tomography and angiography enables delineation of lateral margins of endoscopic mucosal resection: a case report
- Author
-
Benjamin Potsaid, Marisa Figueiredo, Kaicheng Liang, Vijaysekhar Jayaraman, Hiroshi Mashimo, Zhao Wang, Osman O. Ahsen, James G. Fujimoto, Qin Huang, and Hsiang-Chieh Lee
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,Endoscopic mucosal resection ,03 medical and health sciences ,0302 clinical medicine ,Optical coherence tomography ,030220 oncology & carcinogenesis ,Angiography ,medicine ,lcsh:Diseases of the digestive system. Gastroenterology ,030211 gastroenterology & hepatology ,Radiology ,lcsh:RC799-869 ,business ,Letter to the Editor - Published
- 2017
37. Corrigendum to 'A method for ex-post benefit-cost assessment for engineering remediation of debris flow impacts' [Engineering Geology 286 (2021) 106084]
- Author
-
Hsiang-Chieh Lee, Hongey Chen, Hsiao-Yuan Yin, Chih-Hsin Chang, Mei-Chun Lin, and Ming-Jen Chuang
- Subjects
Cost assessment ,Petroleum engineering ,Environmental remediation ,Engineering geology ,Environmental science ,Geology ,Geotechnical Engineering and Engineering Geology ,Debris flow - Published
- 2021
38. Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system
- Author
-
Yun Ru Liu, Kuang Yu Hsu, Wei Hsiang Huang, Sey En Lin, Chien Chung Tsai, D. Y. Jheng, and Hsiang-Chieh Lee
- Subjects
Materials science ,Optical sectioning ,Fresh Tissue ,Image quality ,Fluorescence microscope ,Microscopic imaging ,RGB color model ,Coherence (signal processing) ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Biotechnology ,Biomedical engineering - Abstract
A technique using Linnik-based optical coherence microscopy (OCM), with built-in fluorescence microscopy (FM), is demonstrated here to describe cellular-level morphology for fresh porcine and biobank tissue specimens. The proposed method utilizes color-coding to generate digital pseudo-H&E (p-H&E) images. Using the same camera, colocalized FM images are merged with corresponding morphological OCM images using a 24-bit RGB composition process to generate position-matched p-H&E images. From receipt of dissected fresh tissue piece to generation of stitched images, the total processing time is 2 specimen, which is on average two times faster than frozen-section H&E process for fatty or water-rich fresh tissue specimens. This technique was successfully used to scan human and animal fresh tissue pieces, demonstrating its applicability for both biobank and veterinary purposes. We provide an in-depth comparison between p-H&E and human frozen-section H&E images acquired from the same metastatic sentinel lymph node slice (∼10 µm thick), and show the differences, like elastic fibers of a tiny blood vessel and cytoplasm of tumor cells. This optical sectioning technique provides histopathologists with a convenient assessment method that outputs large-field H&E-like images of fresh tissue pieces without requiring any physical embedment.
- Published
- 2021
39. A method for ex-post benefit-cost assessment for engineering remediation of debris flow impacts
- Author
-
Chih-Hsin Chang, Hsiang-Chieh Lee, Hongey Chen, Mei-Chun Lin, Hsiao-Yuan Yin, and Ming-Jen Chuang
- Subjects
Government ,Environmental remediation ,business.industry ,Southern taiwan ,0211 other engineering and technologies ,Geology ,02 engineering and technology ,Environmental economics ,010502 geochemistry & geophysics ,Geotechnical Engineering and Engineering Geology ,01 natural sciences ,Debris flow ,Actual cost ,Cost assessment ,Assessment methods ,Environmental science ,business ,Risk management ,021101 geological & geomatics engineering ,0105 earth and related environmental sciences - Abstract
Ex-ante and ex-post assessments are both essential in risk management, although ex-post assessments of engineering measures have not been widely reported. In this study, an ex-post benefit-cost assessment method was developed to enable the direct impact of remediation engineering measures for debris flows to be quantified. We propose three categories of parameters for calculating benefit and cost: (1) predicted benefits (comprising predicted benefits of saving lives, at the household level, and of infrastructure protection); (2) actual loss (of losing lives, at the household level, and of infrastructure); (3) actual cost of the engineering. Actual benefit was defined as predicted benefit minus loss after completion of the engineering measure. The engineering measures were regarded as effective when benefits outweighed costs. Government data, survey results based on past disasters, and interview results with specialists were used. This methodology was applied and tested in ten villages located in the Gaoping River Basin in southern Taiwan. The result showed that the range of the benefit and cost ratio was between 1.26 and 7.99. We recommend this ex-post assessment method for rapid screening of districts with low benefit-cost ratios.
- Published
- 2021
40. How does resilience matter? An empirical verification of the relationships between resilience and vulnerability
- Author
-
Thung-Hong Lin, Hsiang-Chieh Lee, and Kuan Hui Elaine Lin
- Subjects
021110 strategic, defence & security studies ,Atmospheric Science ,Actuarial science ,010504 meteorology & atmospheric sciences ,media_common.quotation_subject ,Logit ,0211 other engineering and technologies ,Vulnerability ,Disaster recovery ,02 engineering and technology ,Social class ,01 natural sciences ,Hazard ,Empirical research ,Earth and Planetary Sciences (miscellaneous) ,Household income ,Psychological resilience ,Psychology ,0105 earth and related environmental sciences ,Water Science and Technology ,media_common - Abstract
The theoretical relationships between resilience and vulnerability have long been debated, but limited research has been done to test their relationships from an empirical perspective. This study presents an important case to demonstrate the empirical verification of the relationships. After reviewing relevant theories, we propose revisions of two widely adopted disaster risk and vulnerability formulas and apply them in a Taiwanese case of Typhoon Morakot. The data incorporate four natural hazard data sets, a Taiwan Social Change Survey of nonvictims as the reference group, and a longitudinal data set of Social Impact and Recovery Survey for Typhoon Morakot victims (2010–2012). With those data, two sets of models were constructed based on the two revised formulas. The first set of models estimates a disaster risk, defined as the probability and expected value of victimization determined by the typhoon hazard, household’s exposure, and contextual vulnerability composed of social class, ethnicity, education, and family status. The second set of models estimates an affected household’s outcome vulnerability, defined as the continuous trajectory of household living condition consisted of exposure, contextual vulnerability, and resilience. In the second set, outcome vulnerability is measured through household income pre- and post-disaster to depict the impact and dynamism; resilience is measured through social capital variables. Logit, ordinal linear regression (OLS), and fixed-effect regression were applied to statistically estimate the models. The results highlight that contextual vulnerability deteriorates the disaster risk of typhoon. Resilience has an impact on outcome vulnerability, but its effect is uncertain, likely to be restricted in the disaster recovery.
- Published
- 2017
41. The Single Software Architecture Supporting Fourier Domain Optical Coherence Tomography System
- Author
-
Ting-Hao Chen, Yin-Peng Huang, Ting-Yen Tsai, Yi-Ping Hung, Hsiang-Chieh Lee, and Chuan-Bor Chueh
- Subjects
Data acquisition ,Parallel processing (DSP implementation) ,Optical coherence tomography ,medicine.diagnostic_test ,Computer science ,business.industry ,medicine ,Architecture ,business ,Software architecture ,Fourier domain ,Computer hardware - Abstract
We developed a software architecture utilizing the MFC library, allowing OCT imaging/data acquisition in a synchronized fashion. This architecture supports both version of the Fourier-domain OCT frameworks, including the spectral-domain and the swept-source OCT.
- Published
- 2019
42. Early detection of enamel demineralization by optical coherence tomography
- Author
-
Ting Wei Yeh, Meng-Tsan Tsai, Yen Li Wang, Jia Ling Ke, Hsiang-Chieh Lee, Y. J. Lee, and Wen Ju Chen
- Subjects
Materials science ,Scanning electron microscope ,Optical spectroscopy ,lcsh:Medicine ,01 natural sciences ,Article ,010309 optics ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Optical coherence tomography ,stomatognathic system ,0103 physical sciences ,medicine ,Surface roughness ,Humans ,Tooth Erosion ,Optical techniques ,lcsh:Science ,Dental Enamel ,Phosphoric acid ,Tooth Demineralization ,Multidisciplinary ,Enamel paint ,medicine.diagnostic_test ,lcsh:R ,Tooth surface ,030206 dentistry ,Demineralization ,stomatognathic diseases ,Early Diagnosis ,chemistry ,visual_art ,visual_art.visual_art_medium ,lcsh:Q ,Tomography ,Tomography, Optical Coherence ,Biomedical engineering - Abstract
Enamel is the outermost layer of the tooth that protects it from invasion. In general, an acidic environment accelerates tooth demineralization, leading to the formation of cavities. Scanning electron microscopy (SEM) is conventionally used as an in vitro tool for the observation of tooth morphology changes with acid attacks. Yet, SEM has intrinsic limitations for the potential application of in vivo detection in the early demineralization process. In this study, a high-resolution optical coherence tomography (OCT) system with the axial and transverse resolutions of 2.0 and 2.7 μm in teeth has been utilized for characterizing the effect of the acidic environment (simulated by phosphoric acid) on the enamel topology. The scattering coefficient and the surface roughness of enamel can be directly derived from the OCT results, enabling a quantitative evaluation of the topology changes with demineralization. The dynamic process induced by the acid application is also recorded and analyzed with OCT, depicting the evolution of the demineralization process on enamel. Notably, the estimated enamel scattering coefficient and surface roughness significantly increase with the application time of acid and the results illustrate that the values of both parameters after demineralization are significantly larger than those obtained before the demineralization, illustrating both parameters could be effective to differentiate the healthy and demineralized teeth and determine the severity. The obtained results unambiguously illustrate that demineralization of the tooth surface can be successfully detected by OCT and further used as an indicator of early-stage cavity formation.
- Published
- 2019
43. A luminal unfolding microneedle injector for oral delivery of macromolecules
- Author
-
Joy Collins, Ulrik Lytt Rahbek, Tadayuki Yoshitake, Robert Langer, Giovanni Traverso, Alison Hayward, Niclas Roxhed, Yuan Gao, David Dellal, Hsiang-Chieh Lee, Soyoung Kim, Ester Caffarel-Salvador, Johannes Josef Fels, Daniel Minahan, Abramson Alex G, Morten Revsgaard Frederiksen, Jacob Wainer, Siddartha Tamang, James G. Fujimoto, Vance Soares, Xiaoya Lu, and Ryan Yu Tian
- Subjects
0301 basic medicine ,Drug ,Swine ,medicine.medical_treatment ,media_common.quotation_subject ,Pharmacology ,Administration, Cutaneous ,General Biochemistry, Genetics and Molecular Biology ,Dosage form ,Article ,03 medical and health sciences ,Subcutaneous injection ,0302 clinical medicine ,Drug Delivery Systems ,Pharmacokinetics ,In vivo ,Medicine ,Animals ,Humans ,Insulin ,media_common ,business.industry ,Capsule ,General Medicine ,Equipment Design ,030104 developmental biology ,Needles ,030220 oncology & carcinogenesis ,business ,Ex vivo - Abstract
Insulin and other injectable biologic drugs have transformed the treatment of patients suffering from diabetes1,2, yet patients and healthcare providers often prefer to use and prescribe less effective orally dosed medications3-5. Compared with subcutaneously administered drugs, oral formulations create less patient discomfort4, show greater chemical stability at high temperatures6, and do not generate biohazardous needle waste7. An oral dosage form for biologic medications is ideal; however, macromolecule drugs are not readily absorbed into the bloodstream through the gastrointestinal tract8. We developed an ingestible capsule, termed the luminal unfolding microneedle injector, which allows for the oral delivery of biologic drugs by rapidly propelling dissolvable drug-loaded microneedles into intestinal tissue using a set of unfolding arms. During ex vivo human and in vivo swine studies, the device consistently delivered the microneedles to the tissue without causing complete thickness perforations. Using insulin as a model drug, we showed that, when actuated, the luminal unfolding microneedle injector provided a faster pharmacokinetic uptake profile and a systemic uptake >10% of that of a subcutaneous injection over a 4-h sampling period. With the ability to load a multitude of microneedle formulations, the device can serve as a platform to orally deliver therapeutic doses of macromolecule drugs.
- Published
- 2018
44. High-stability inorganic perovskite quantum dot–cellulose nanocrystal hybrid films
- Author
-
Chiu-Yen Wang, Y. J. Lee, Liang Feng Tsai, Han Song Wu, Kun You Li, Chiao Fang Hsu, Hsiang-Chieh Lee, Chih Hao Chiang, Po Kang Yang, Ting-You Li, and Meng-Lin Tsai
- Subjects
Materials science ,Photoluminescence ,Quantum yield ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Oleylamine ,General Materials Science ,Electrical and Electronic Engineering ,Perovskite (structure) ,business.industry ,Mechanical Engineering ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Full width at half maximum ,chemistry ,Nanocrystal ,Mechanics of Materials ,Quantum dot ,Optoelectronics ,Light emission ,0210 nano-technology ,business - Abstract
Inorganic perovskite quantum dots (IPQDs) such as cesium lead halide (CsPbX3, X = Cl, Br and I) quantum dots have attracted much attention for developing cadmium-free quantum light-emitting displays (QLEDs) based on outstanding light emission properties including narrow full width at half maximum (FWHM), tunable bandgap and ultrahigh (>90%) photoluminescence quantum yield (PLQY). Nevertheless, their poor stability under ambient conditions, at high temperature or under continuous light irradiation is the main problem for practical applications. In this study, a new method is proposed to effectively stabilize CsPbBr3 IPQDs by synthesizing them with sulfate-functionalized cellulose nanocrystals (CNCs) at room temperature without using traditional quantum dot stabilizers such as oleylamine (OLA) and oleic acid (OA). The as-prepared CsPbBr3 IPQD/CNC hybrid paper-like films are highly stable and the relative photoluminescence (PL) intensity can be maintained at 92% under continuous UV light (306 nm, 15 W) illumination for 130 h, >99% at high temperature (100 °C) for 130 h, and >99% in ambient conditions for 15 d. Additionally, the PLQY and FWHM of IPQD/CNC are 45.69% and 22 nm, respectively. The ultrahigh stability and narrow FWHM characteristics proposed here for IPQD/CNC hybrid films can provide new possibilities for practical applications in the future development of IPQD-related devices.
- Published
- 2020
45. Implementing the Sendai Framework for disaster risk reduction 2015–2030: Disaster governance strategies for persons with disabilities in Taiwan
- Author
-
Hongey Chen and Hsiang-Chieh Lee
- Subjects
021110 strategic, defence & security studies ,010504 meteorology & atmospheric sciences ,Disaster risk reduction ,business.industry ,Corporate governance ,media_common.quotation_subject ,0211 other engineering and technologies ,Stakeholder ,Disaster recovery ,Geology ,02 engineering and technology ,Public relations ,Geotechnical Engineering and Engineering Geology ,01 natural sciences ,Emergency response ,Backup ,Preparedness ,Business ,Empowerment ,Safety Research ,0105 earth and related environmental sciences ,media_common - Abstract
This study examined how to effectively implement the Sendai Framework for Disaster Risk Reduction 2015–2030 by inspecting disaster governance strategies for persons with disabilities in Taiwan. We identified priorities of the Sendai Framework that especially mention persons with disabilities or related stakeholders, including Priorities 24(a), 24(g), 24(h), 27(g), 27(h), 30(j), 30(k), 33(b), and 33(i). A set of questions was designed according to these priorities and distributed to all central ministries, agencies, and local governments related to disaster governance in Taiwan. The results showed that strategies currently cover the issues of evacuation, shelter operation, facility operation for persons with disabilities, electricity backup during emergency, strategies for schools, and disaster recovery and reconstruction. We found that the present disaster governance strategies for persons with disabilities in Taiwan are focused on the preparedness and emergency response phases, and most strategies utilize a top-down approach. Based on these findings, we suggest the development of more strategies for the recovery and reconstruction phases and the incorporation of bottom-up mechanisms such as stakeholder participation, dialogue and cooperation, coordination forums, empowerment, and consultation with stakeholders, as discussed in Priorities 24(h), 27(g), 30(j), and 33(i) of the Sendai Framework.
- Published
- 2019
46. Cycloid scanning for wide field optical coherence tomography endomicroscopy and angiography
- Author
-
Benjamin Potsaid, Xingde Li, Alex Cable, Zhao Wang, Vijaysekhar Jayaraman, Hiroshi Mashimo, Hsiang-Chieh Lee, Osman O. Ahsen, Kaicheng Liang, James G. Fujimoto, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, and Massachusetts Institute of Technology. Research Laboratory of Electronics
- Subjects
Scanner ,Materials science ,medicine.diagnostic_test ,genetic structures ,Image quality ,Microscanning ,Confocal ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,eye diseases ,Article ,Electronic, Optical and Magnetic Materials ,010309 optics ,Optical coherence tomography ,0103 physical sciences ,Angiography ,Endomicroscopy ,medicine ,sense organs ,0210 nano-technology ,Preclinical imaging ,Biomedical engineering - Abstract
Devices that perform wide field-of-view (FOV) precision optical scanning are important for endoscopic assessment and diagnosis of luminal organ disease such as in gastroenterology. Optical scanning for in vivo endoscopic imaging has traditionally relied on one or more proximal mechanical actuators, limiting scan accuracy and imaging speed. There is a need for rapid and precise two-dimensional (2D) microscanning technologies to enable the translation of benchtop scanning microscopies to in vivo endoscopic imaging. We demonstrate a new cycloid scanner in a tethered capsule for ultrahigh speed, side-viewing optical coherence tomography (OCT) endomicroscopy in vivo. The cycloid capsule incorporates two scanners: a piezoelectrically actuated resonant fiber scanner to perform a precision, small FOV, fast scan and a micromotor scanner to perform a wide FOV, slow scan. Together these scanners distally scan the beam circumferentially in a 2D cycloid pattern, generating an unwrapped1 mm× 38 mm strip FOV. Sequential strip volumes can be acquired with proximal pullback to image centimeter-long regions. Using ultrahigh speed 1.3 μm wavelength swept-source OCT at a 1.17 MHz axial scan rate, we imaged the human rectum at 3 volumes/s. Each OCT strip volume had 166 × 2322 axial scans with 8.5 μm axial and 30 μm transverse resolution. We further demonstrate OCT angiography at 0.5 volumes/s, producing volumetric images of vasculature. In addition to OCT applications, cycloid scanning promises to enable precision 2D optical scanning for other imaging modalities, including fluorescence confocal and nonlinear microscopy., National Institutes of Health (U.S.) (Grant R01-CA075289), National Institutes of Health (U.S.) (Grant R01-CA178636), National Institutes of Health (U.S.) (Grant R01-EY011289), National Institutes of Health (U.S.) (Grant R44-CA101067), Air Force Office of Scientific Research (Grant FA9550-12-1- 0499), Air Force Office of Scientific Research (Grant FA9550-15-1-0473)
- Published
- 2018
47. Endoscopic optical coherence tomography and angiography for gastroenterology applications
- Author
-
Marisa Figueiredo, Vijaysekhar Jayaraman, Benjamin Potsaid, James G. Fujimoto, Osman O. Ahsen, Zhao Wang, Hiroshi Mashimo, Kaicheng Liang, Qin Huang, and Hsiang-Chieh Lee
- Subjects
medicine.medical_specialty ,genetic structures ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,eye diseases ,Endoscopy ,Oct angiography ,Optical coherence tomography ,Internal medicine ,Angiography ,medicine ,sense organs ,business ,Preclinical imaging - Abstract
Endoscopic optical coherence tomography (OCT) angiography enables volumetric coregistered architectural and microvasculature imaging of the human gastrointestinal tract in vivo. In this talk, we will discuss technical advances and clinical gastroenterology applications with the endoscopic OCT angiography technique.
- Published
- 2018
48. Texture analyses of optical coherence tomography images obtained during tumor development
- Author
-
Hsiang-Chieh Lee and Meng-Tsan Tsai
- Subjects
Materials science ,Optical coherence tomography ,medicine.diagnostic_test ,business.industry ,medicine ,Computer vision ,Development (differential geometry) ,Artificial intelligence ,business ,Texture (geology) - Published
- 2018
49. Integrated Local Binary Pattern Texture Features for Classification of Breast Tissue Imaged by Optical Coherence Microscopy
- Author
-
Yuri Sheikine, Tao Xu, Sunhua Wan, Ting Xu, Xiaolei Huang, James G. Fujimoto, Chao Zhou, Zhan Zhang, Xianxu Zeng, Hsiang-Chieh Lee, and James L. Connolly
- Subjects
Computer science ,Local binary patterns ,Lobular carcinoma ,Health Informatics ,Feature selection ,Breast Neoplasms ,02 engineering and technology ,01 natural sciences ,Sensitivity and Specificity ,Article ,Pattern Recognition, Automated ,010309 optics ,0103 physical sciences ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,Image Processing, Computer-Assisted ,Humans ,Radiology, Nuclear Medicine and imaging ,Computer vision ,Breast ,Microscopy, Confocal ,Radiological and Ultrasound Technology ,Receiver operating characteristic ,Pixel ,business.industry ,Reproducibility of Results ,Ductal carcinoma ,medicine.disease ,Computer Graphics and Computer-Aided Design ,Fibroadenoma ,ROC Curve ,Feature (computer vision) ,020201 artificial intelligence & image processing ,Female ,Computer Vision and Pattern Recognition ,Artificial intelligence ,business ,Algorithms ,Tomography, Optical Coherence - Abstract
This paper proposes a texture analysis technique that can effectively classify different types of human breast tissue imaged by Optical Coherence Microscopy (OCM). OCM is an emerging imaging modality for rapid tissue screening and has the potential to provide high resolution microscopic images that approach those of histology. OCM images, acquired without tissue staining, however, pose unique challenges to image analysis and pattern classification. We examined multiple types of texture features and found Local Binary Pattern (LBP) features to perform better in classifying tissues imaged by OCM. In order to improve classification accuracy, we propose novel variants of LBP features, namely average LBP (ALBP) and block based LBP (BLBP). Compared with the classic LBP feature, ALBP and BLBP features provide an enhanced encoding of the texture structure in a local neighborhood by looking at intensity differences among neighboring pixels and among certain blocks of pixels in the neighborhood. Fourty-six freshly excised human breast tissue samples, including 27 benign (e.g. fibroadenoma, fibrocystic disease and usual ductal hyperplasia) and 19 breast carcinoma (e.g. invasive ductal carcinoma, ductal carcinoma in situ and lobular carcinoma in situ) were imaged with large field OCM with an imaging area of 10 × 10 mm2 (10, 000 × 10, 000 pixels) for each sample. Corresponding H&E histology was obtained for each sample and used to provide ground truth diagnosis. 4310 small OCM image blocks (500 × 500 pixels) each paired with corresponding H&E histology was extracted from large-field OCM images and labeled with one of the five different classes: adipose tissue (n = 347), fibrous stroma (n = 2,065), breast lobules (n = 199), carcinomas (pooled from all sub-types, n = 1,127), and background (regions outside of the specimens, n = 572). Our experiments show that by integrating a selected set of LBP and the two new variant (ALBP and BLBP) features at multiple scales, the classification accuracy increased from 81.7% (using LBP features alone) to 93.8% using a neural network classifier. The integrated feature was also used to classify large-field OCM images for tumor detection. A receiver operating characteristic (ROC) curve was obtained with an area under the curve value of 0.959. A sensitivity level of 100% and specificity level of 85.2% was achieved to differentiate benign from malignant samples. Several other experiments also demonstrate the complementary nature of LBP and the two variants (ALBP and BLBP features) and the significance of integrating these texture features for classification. Using features from multiple scales and performing feature selection are also effective mechanisms to improve accuracy while maintaining computational efficiency.
- Published
- 2017
50. Volumetric Mapping of Barrett’s Esophagus and Dysplasia With en face Optical Coherence Tomography Tethered Capsule
- Author
-
James G. Fujimoto, Marisa Figueiredo, Vijaysekhar Jayaraman, Zhao Wang, Hiroshi Mashimo, Kaicheng Liang, Benjamin Potsaid, Alex Cable, Qin Huang, Hsiang-Chieh Lee, and Osman O. Ahsen
- Subjects
medicine.medical_specialty ,genetic structures ,digestive system ,01 natural sciences ,Article ,010309 optics ,03 medical and health sciences ,0302 clinical medicine ,Optical coherence tomography ,0103 physical sciences ,medicine ,Esophagus ,Capsule Endoscopes ,Hepatology ,medicine.diagnostic_test ,Extramural ,business.industry ,Gastroenterology ,Capsule ,Anatomy ,medicine.disease ,eye diseases ,digestive system diseases ,surgical procedures, operative ,medicine.anatomical_structure ,Dysplasia ,Barrett's esophagus ,030211 gastroenterology & hepatology ,Radiology ,Tomography ,business - Abstract
Volumetric Mapping of Barrett’s Esophagus and Dysplasia With en face Optical Coherence Tomography Tethered Capsule
- Published
- 2016
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.