14 results on '"Hokke, Cornelis H."'
Search Results
2. Screening of a library of recombinant Schistosoma mansoni proteins with sera from murine and human controlled infections identifies early serological markers
- Author
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Crosnier, Cécile, Hokke, Cornelis H, Protasio, Anna V, Brandt, Cordelia, Rinaldi, Gabriel, Langenberg, Marijke C C, Clare, Simon, Janse, Jacqueline J, Wilson, Shona, Berriman, Matthew, Roestenberg, Meta, and Wright, Gavin J
- Subjects
parasitic diseases - Abstract
Schistosomiasis is a major global health problem caused by blood-dwelling parasitic worms, which is currently tackled primarily by mass administration of the drug praziquantel. Appropriate drug treatment strategies are informed by diagnostics that establish the prevalence and intensity of infection, which, in regions of low transmission, should be highly sensitive. To identify sensitive new serological markers of Schistosoma mansoni infections, we have compiled a recombinant protein library of parasite cell-surface and secreted proteins expressed in mammalian cells. Together with a time series of sera samples from volunteers experimentally infected with a defined number of male parasites, we probed this protein library to identify several markers that can detect primary infections with as low as ten parasites and as early as five weeks post infection. These new markers could be further explored as valuable tools to detect ongoing and previous S. mansoni infections, including in endemic regions where transmission is low. © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.
- Published
- 2020
3. Schistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses
- Author
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Egesa, Moses, Lubyayi, Lawrence, Tukahebwa, Edridah M, Bagaya, Bernard S, Chalmers, Iain W, Wilson, Shona, Hokke, Cornelis H, Hoffmann, Karl F, Dunne, David W, Yazdanbakhsh, Maria, Labuda, Lucja A, Cose, Stephen, Cose, Stephen [0000-0002-5156-037X], and Apollo - University of Cambridge Repository
- Subjects
Anthelmintics ,Male ,Immunity, Cellular ,Th1/pro-inflammatory ,Schistosoma mansoni ,Th1 Cells ,cytokines ,Praziquantel ,Schistosomiasis mansoni ,schistosomula ,vaccine ,Antigens, Helminth ,Larva ,parasitic diseases ,Leukocytes, Mononuclear ,Animals ,Humans ,Female - Abstract
Larvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni-infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens (AWA and SEA), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at 1 year after praziquantel (PZQ) treatment using the Kato-Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment was investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro-inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro-inflammatory response, they had an association with pre-treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune-epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.
- Published
- 2018
4. Schistosoma mansoni schistosomula antigens induce Th1/Pro‐inflammatory cytokine responses
- Author
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Egesa, Moses, Lubyayi, Lawrence, Tukahebwa, Edridah M., Bagaya, Bernard S., Chalmers, Iain W., Wilson, Shona, Hokke, Cornelis H., Hoffmann, Karl F., Dunne, David W., Yazdanbakhsh, Maria, Labuda, Lucja A., and Cose, Stephen
- Subjects
Anthelmintics ,Male ,Immunity, Cellular ,Original Articles ,Schistosoma mansoni ,Th1 Cells ,cytokines ,Th1/pro‐inflammatory ,Praziquantel ,Schistosomiasis mansoni ,schistosomula ,vaccine ,Antigens, Helminth ,Larva ,parasitic diseases ,Leukocytes, Mononuclear ,Animals ,Humans ,Original Article ,Female - Abstract
Summary Larvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni‐infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens (AWA and SEA), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at 1 year after praziquantel (PZQ) treatment using the Kato‐Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment was investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro‐inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro‐inflammatory response, they had an association with pre‐treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune‐epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.
- Published
- 2018
5. Novel O-linked methylated glycan antigens decorate secreted immunodominant glycoproteins from the intestinal nematode Heligmosomoides polygyrus
- Author
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Hewitson, James P., Nguyen, D. Linh, van Diepen, Angela, Smit, Cornelis H., Koeleman, Carolien A., McSorley, Henry J., Murray, Janice, Maizels, Rick M., and Hokke, Cornelis H.
- Subjects
Male ,Carbohydrate ,Mice, Inbred BALB C ,Nematospiroides dubius ,Mass spectrometry ,Excretory–secretory product ,Heligmosomoides polygyrus ,Helminth Proteins ,Methylation ,Article ,Intestines ,Mice ,Infectious Diseases ,Polysaccharides ,parasitic diseases ,Animals ,Humans ,Parasitology ,Female ,Antibody ,ComputingMethodologies_COMPUTERGRAPHICS ,Nematode ,Glycoproteins ,Strongylida Infections - Abstract
Graphical abstract, Highlights • Heligmosomoides polygyrus excretory–secretory (ES) proteins carry diverse N- and O-glycans, and many are O-methylated. • A methylhexose containing O-glycan of abundant ES glycoproteins is immunodominant. • This dominant glycan is not the immunomodulatory heat-stable ES component., Glycan molecules from helminth parasites have been associated with diverse biological functions ranging from interactions with neighbouring host cell populations to down-modulation of specific host immunity. Glycoproteins secreted by the intestinal nematode Heligmosomoides polygyrus are of particular interest as the excretory–secretory products (termed HES) of this parasite contain both heat-labile and heat-stable components with immunomodulatory effects. We used MALDI-TOF-MS and LC–MS/MS to analyse the repertoire of N- and O-linked glycans released from Heligmosomoides polygyrus excretory–secretory products by PNGase A and F, β-elimination and hydrazinolysis revealing a broad range of structures including novel methylhexose- and methylfucose-containing glycans. Monoclonal antibodies to two immunodominant glycans of H. polygyrus, previously designated Glycans A and B, were found to react by glycan array analysis to a methyl-hexose-rich fraction and to a sulphated LacDiNAc (LDN; GalNAcβ1–4GlcNAc) structure, respectively. We also analysed the glycan repertoire of a major glycoprotein in Heligmosomoides polygyrus excretory–secretory products, VAL-2, which contains many glycan structures present in Heligmosomoides polygyrus excretory–secretory products including Glycan A. However, it was found that this set of glycans is not responsible for the heat-stable immunomodulatory properties of Heligmosomoides polygyrus excretory–secretory products, as revealed by the inability of VAL-2 to inhibit allergic lung inflammation. Taken together, these studies reveal that H. polygyrus secretes a diverse range of antigenic glycoconjugates, and provides a framework to explore the biological and immunomodulatory roles they may play within the mammalian host.
- Published
- 2016
6. Antibody responses to Schistosoma mansoni schistosomula antigens
- Author
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Egesa, Moses, Lubyayi, Lawrence, Jones, Frances M, van Diepen, Angela, Chalmers, Iain W, Tukahebwa, Edridah M, Bagaya, Bernard S, Hokke, Cornelis H, Hoffmann, Karl F, Dunne, David W, Elliott, Alison M, Yazdanbakhsh, Maria, Wilson, Shona, Cose, Stephen, Cose, Stephen [0000-0002-5156-037X], and Apollo - University of Cambridge Repository
- Subjects
Anthelmintics ,Male ,Antibodies, Helminth ,Enzyme-Linked Immunosorbent Assay ,Schistosoma mansoni ,Helminth Proteins ,Immunoglobulin E ,antibody responder ,Praziquantel ,Schistosomiasis mansoni ,reinfection ,schistosomula antigens ,Antigens, Helminth ,Immunoglobulin G ,Antibody Formation ,parasitic diseases ,Animals ,Humans ,Female ,Uganda ,IgE - Abstract
While antigens from Schistosoma schistosomula have been suggested as potential vaccine candidates, the association between antibody responses with schistosomula antigens and infection intensity at reinfection is not well known. Schistosoma mansoni-infected individuals were recruited from a schistosomiasis endemic area in Uganda (n = 372), treated with 40 mg/kg praziquantel (PZQ) and followed up at five weeks and at one year post-treatment. Pre-treatment and five weeks post-treatment immunoglobulin (Ig) E, IgG1 and IgG4 levels against recombinant schistosomula antigens rSmKK7, rSmLy6A, rSmLy6B and rSmTSP7 were measured using ELISA. Factors associated with detectable pre-treatment or post-treatment antibody response against the schistosomula antigens and the association between five-week antibody responses and one year post-treatment reinfection intensity among antibody responders were examined. Being male was associated with higher pre-treatment IgG1 to rSmKK7, rSmLy6a and AWA. Five weeks post-treatment antibody responses against schistosomula antigens were not associated with one year post-treatment reinfection intensity among antibody responders' antibody levels against rSmKK7, rSmLy6B and rSmTSP7 dropped, but increased against rSmLy6A, AWA and SEA at five weeks post-treatment among antibody responders. S. mansoni-infected individuals exhibit detectable antibody responses to schistosomula antigens that are affected by treatment. These findings indicate that schistosomula antigens induce highly varied antibody responses and could have implications for vaccine development.
- Published
- 2018
7. Rethinking Schistosomiasis Vaccine Development: Synthetic Vesicles
- Author
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Egesa, Moses, Hoffmann, Karl F, Hokke, Cornelis H, Yazdanbakhsh, Maria, and Cose, Stephen
- Subjects
parasitic diseases - Abstract
There is currently no vaccine against schistosomiasis. With few Schistosoma vaccine candidates in clinical trials, unexplored antigens from the vulnerable schistosomulum should be considered as possible vaccine candidates. In addition, we suggest developing synthetic vesicles as a new delivery vehicle and adjuvant for immunoprophylactic schistosomula vaccine candidates.
- Published
- 2017
8. Binding of von Willebrand factor and plasma proteins to the eggshell of Schistosoma mansoni
- Author
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Dewalick, Saskia, Hensbergen, Paul J, Bexkens, Michiel L, Grosserichter-Wagener, Christina, Hokke, Cornelis H, Deelder, André M, de Groot, Philip G, Tielens, Aloysius G M, van Hellemond, Jaap J, B&C BRC-SIB-TR, LS Biochemie van parasieten, Strategic Infection Biology, B&C BRC-SIB-TR, LS Biochemie van parasieten, Strategic Infection Biology, and Medical Microbiology & Infectious Diseases
- Subjects
Adult ,Endothelium ,Zygote ,Plasma protein binding ,Von Willebrand factor ,Blood plasma ,parasitic diseases ,von Willebrand Factor ,medicine ,Schistosomiasis ,Animals ,Humans ,Binding site ,Eggshell ,Sequence Deletion ,Binding Sites ,biology ,Plasma proteins ,Blood Proteins ,Schistosoma mansoni ,biology.organism_classification ,Blood proteins ,Egg excretion ,Cell biology ,Infectious Diseases ,medicine.anatomical_structure ,Immunology ,biology.protein ,Parasitology ,Haemostasis ,Protein Binding - Abstract
Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end. (C) 2014 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
- Published
- 2014
9. IgG1 Fc N-glycan galactosylation as a biomarker for immune activation
- Author
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De Jong, Sanne E., Selman, Maurice H J, Adegnika, Ayola A., Amoah, Abena S., Van Riet, Elly, Kruize, Yvonne C M, Raynes, John G., Rodriguez, Alejandro, Boakye, Daniel, Von Mutius, Erika, Knulst, André C., Genuneit, Jon, Cooper, Philip J., Hokke, Cornelis H., Wuhrer, Manfred, and Yazdanbakhsh, Maria
- Subjects
Male ,0301 basic medicine ,Glycan ,Glycosylation ,Adolescent ,Inflammation ,Receptors, Fc ,Communicable Diseases ,Ghana ,Article ,Immunoglobulin G ,03 medical and health sciences ,Polysaccharides ,Germany ,Journal Article ,medicine ,Animals ,Humans ,Schistosomiasis ,Gabon ,Effector functions ,Child ,Receptor ,Netherlands ,Multidisciplinary ,biology ,Total ige ,Immunoglobulin E ,3. Good health ,carbohydrates (lipids) ,C-Reactive Protein ,030104 developmental biology ,Child, Preschool ,Immunology ,biology.protein ,Schistosoma ,Biomarker (medicine) ,Female ,lipids (amino acids, peptides, and proteins) ,Ecuador ,medicine.symptom ,Biomarkers ,Immune activation - Abstract
Immunoglobulin G (IgG) Fc N-glycosylation affects antibody-mediated effector functions and varies with inflammation rooted in both communicable and non-communicable diseases. Worldwide, communicable and non-communicable diseases tend to segregate geographically. Therefore, we studied whether IgG Fc N-glycosylation varies in populations with different environmental exposures in different parts of the world. IgG Fc N-glycosylation was analysed in serum/plasma of 700 school-age children from different communities of Gabon, Ghana, Ecuador, the Netherlands and Germany. IgG1 galactosylation levels were generally higher in more affluent countries and in more urban communities. High IgG1 galactosylation levels correlated with low total IgE levels, low C-reactive protein levels and low prevalence of parasitic infections. Linear mixed modelling showed that only positivity for parasitic infections was a significant predictor of reduced IgG1 galactosylation levels. That IgG1 galactosylation is a predictor of immune activation is supported by the observation that asthmatic children seemed to have reduced IgG1 galactosylation levels as well. This indicates that IgG1 galactosylation levels could be used as a biomarker for immune activation of populations, providing a valuable tool for studies examining the epidemiological transition from communicable to non-communicable diseases.
- Published
- 2016
10. Novel O-linked methylated glycan antigens decorate secreted immunodominant glycoproteins from the intestinal nematode Heligmosomoides polygyrus
- Author
-
Hewitson, James P., Nguyen, D. Linh, van Diepen, Angela, Smit, Cornelis H., Koeleman, Carolien A., McSorley, Henry J., Murray, Janice, Maizels, Rick M., and Hokke, Cornelis H.
- Subjects
carbohydrates (lipids) ,Carbohydrate ,Excretory-secretory product ,Infectious Diseases ,Mass spectrometry ,Heligmosomoides polygyrus ,Parasitology ,Antibody ,Nematode - Abstract
Glycan molecules from helminth parasites have been associated with diverse biological functions ranging from interactions with neighbouring host cell populations to down-modulation of specific host immunity. Glycoproteins secreted by the intestinal nematode Heligmosomoides polygyrus are of particular interest as the excretory–secretory products (termed HES) of this parasite contain both heat-labile and heat-stable components with immunomodulatory effects. We used MALDI-TOF-MS and LC–MS/MS to analyse the repertoire of HES N- and O-linked glycans released by PNGase A and F, β-elimination and hydrazinolysis revealing a broad range of structures including novel methylhexose- and methylfucose-containing glycans. Monoclonal antibodies to two immunodominant glycans of H. polygyrus, previously designated Glycans A and B, were found to react by glycan array analysis to a methyl-hexose-rich fraction and to a sulphated LacDiNAc (LDN; GalNAcβ1–4GlcNAc) structure, respectively. We also analysed the glycan repertoire of a major HES glycoprotein, VAL-2, which contains many glycan structures present in HES including Glycan A. However, it was found that this set of glycans is not responsible for the heat-stable immunomodulatory properties of HES, as revealed by the inability of VAL-2 to inhibit allergic lung inflammation. Taken together, these studies reveal that H. polygyrus secretes a diverse range of antigenic glycoconjugates, and provides a framework to explore the biological and immunomodulatory roles they may play within the mammalian host.
- Published
- 2016
11. Phase Variation in Helicobacter pylori Lipopolysaccharide due to Changes in the Lengths of Poly(C) Tracts in α3-Fucosyltransferase Genes
- Author
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Appelmelk, Ben J., Martin, Steve L., Monteiro, Mario A., Clayton, Chris A., McColm, Andrew A., Zheng, Pengyuan, Verboom, Theo, Maaskant, Janneke J., van den Eijnden, Dirk H., Hokke, Cornelis H., Perry, Malcolm B., Vandenbroucke-Grauls, Christina M. J. E., and Kusters, Johannes G.
- Subjects
Errata - Published
- 1999
12. Antibody responses to Schistosoma mansoni schistosomula antigens
- Author
-
Egesa, Moses, Lubyayi, Lawrence, Jones, Frances M, Van Diepen, Angela, Chalmers, Iain W, Tukahebwa, Edridah M, Bagaya, Bernard S, Hokke, Cornelis H, Hoffmann, Karl F, Dunne, David W, Elliott, Alison M, Yazdanbakhsh, Maria, Wilson, Shona, and Cose, Stephen
- Subjects
Anthelmintics ,Male ,Antibodies, Helminth ,Enzyme-Linked Immunosorbent Assay ,Schistosoma mansoni ,Helminth Proteins ,Immunoglobulin E ,antibody responder ,Praziquantel ,Schistosomiasis mansoni ,3. Good health ,reinfection ,schistosomula antigens ,Antigens, Helminth ,Immunoglobulin G ,parasitic diseases ,Antibody Formation ,Animals ,Humans ,Female ,Uganda ,IgE - Abstract
While antigens from Schistosoma schistosomula have been suggested as potential vaccine candidates, the association between antibody responses with schistosomula antigens and infection intensity at reinfection is not well known. Schistosoma mansoni-infected individuals were recruited from a schistosomiasis endemic area in Uganda (n = 372), treated with 40 mg/kg praziquantel (PZQ) and followed up at five weeks and at one year post-treatment. Pre-treatment and five weeks post-treatment immunoglobulin (Ig) E, IgG1 and IgG4 levels against recombinant schistosomula antigens rSmKK7, rSmLy6A, rSmLy6B and rSmTSP7 were measured using ELISA. Factors associated with detectable pre-treatment or post-treatment antibody response against the schistosomula antigens and the association between five-week antibody responses and one year post-treatment reinfection intensity among antibody responders were examined. Being male was associated with higher pre-treatment IgG1 to rSmKK7, rSmLy6a and AWA. Five weeks post-treatment antibody responses against schistosomula antigens were not associated with one year post-treatment reinfection intensity among antibody responders' antibody levels against rSmKK7, rSmLy6B and rSmTSP7 dropped, but increased against rSmLy6A, AWA and SEA at five weeks post-treatment among antibody responders. S. mansoni-infected individuals exhibit detectable antibody responses to schistosomula antigens that are affected by treatment. These findings indicate that schistosomula antigens induce highly varied antibody responses and could have implications for vaccine development.
13. Schistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses
- Author
-
Egesa, Moses, Lubyayi, Lawrence, Tukahebwa, Edridah M, Bagaya, Bernard S, Chalmers, Iain W, Wilson, Shona, Hokke, Cornelis H, Hoffmann, Karl F, Dunne, David W, Yazdanbakhsh, Maria, Labuda, Lucja A, and Cose, Stephen
- Subjects
Anthelmintics ,Male ,Immunity, Cellular ,Th1/pro-inflammatory ,Schistosoma mansoni ,Th1 Cells ,cytokines ,Praziquantel ,Schistosomiasis mansoni ,3. Good health ,schistosomula ,vaccine ,Antigens, Helminth ,Larva ,parasitic diseases ,Leukocytes, Mononuclear ,Animals ,Humans ,Female - Abstract
Larvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni-infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens (AWA and SEA), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at 1 year after praziquantel (PZQ) treatment using the Kato-Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment was investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro-inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro-inflammatory response, they had an association with pre-treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune-epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.
14. THE DIAGNOSTIC POTENTIAL OF GLYCAN SPECIFIC ANTIBODIES IN SCHISTOSOMIASIS ASSESSED BY GLYCAN MICROARRAYS
- Author
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Kildemoes, Anna O., Diepen, Angela, Veldhuizen, Tom, Linh Nguyen, Tanaka, Mio, Dam, Govert J., Meta Roestenberg, Hamano, Shinjiro, and Hokke, Cornelis H.
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