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Aims Peripheral arterial disease (PAD) is a major public health burden requiring more intensive population screening. Ankle brachial index (ABI) using arm and ankle cuffs is considered as the reference method for the detection of PAD. Although it requires a rigorous methodology by trained operators, it remains time-consuming and more technically difficult in patients with diabetes due to mediacalcosis. Techniques based on the study of hemodynamic, such as the systolic rise time (SRT), appear promising but need to be validated. We retrospectively compared the reliability and accuracy of SRT using a photoplethysmography (PPG) technique to the SRT measured by ultrasound doppler (UD) in PAD patients diagnosed with the ABI (137 patients, 200 lower limbs). Methods and results There was a significant correlation between SRT measured with UD (SRTud) compared with that with PPG (SRTppg, r = 0.25; P = 0.001). Best correlation was found in patients without diabetes (r = 0.40; P = 0.001). Bland and Altman analysis showed a good agreement between the SRTud and SRTppg. In contrast, there was no significant correlation between UD and PPG in diabetes patients. Furthermore, patients with diabetes exhibited a significant increase of SRTppg (P = 0.02) compared with patients without diabates but not with the SRTud (P = 0.18). The SRTppg was significantly linked to the arterial velocity waveforms, the type of arterial lesion but not vascular surgery revascularization technique. Conclusion This monocentric pilot study shows that SRT measured with the PPG signal reliably correlates with SRT recorded with UD. The PPG is an easy to use technique in the hand of non-expert with a potential interest for general screening of PAD, especially in diabetes patients, due to its ease to use.

Arterial stiffness (AS), as assessed via pulse wave velocity (PWV), is a major biomarker for cardiovascular risk assessment in patients with chronic kidney disease (CKD). However, the mechanisms responsible for the changes in PWV in the presence of kidney disease are not yet fully elucidated. In the present study, we aimed to investigate the direct effects attributable to biomechanical changes in the arterial tree caused by staged renal removal, independent of any biochemical or compensatory effects. Particularly, we simulated arterial pressure and flow using a previously validated one-dimensional (1-D) model of the cardiovascular system with different kidney configurations: two kidneys (2KDN), one single kidney (1KDN), no kidneys (0KDN), and a transplanted kidney (TX) attached to the external iliac artery. We evaluated the respective variations in blood pressure (BP), as well as AS of large-, medium-, and small-sized arteries via carotid-femoral PWV (cfPWV), carotid-radial PWV (crPWV), and radial-digital PWV (rdPWV), respectively. Our results showed that BP was increased in 1KDN and 0KDN, and that systolic BP values were restored in the TX configuration. Furthermore, a rise was reported in all PWVs for all tested configurations. The relative difference in stiffness from 2KDN to 0KDN was higher in the case of crPWV (15%) in comparison with the increase observed for cfPWV (11%). In TX, we observed a restoration of the PWVs to values close to 1KDN. Globally, it was demonstrated that alterations of the outflow boundaries to the renal arteries with staged kidney removal led to changes in BP and central and peripheral PWV in line with previously reported clinical data. Our findings suggest that the PWV variations observed in clinical practice with different stages of kidney disease may be partially attributed to biomechanical alterations of the arterial tree and their effect on BP.

Background: Physiologically, the aorta is less stiff than peripheral conductive arteries, creating an arterial stiffness gradient, protecting microcirculation from high pulsatile pressure. However, the pharmacological manipulation of arterial stiffness gradient has not been thoroughly investigated. We hypothesized that acute administration of nitroglycerin (NTG) may alter the arterial stiffness gradient through a more significant effect on the regional stiffness of medium-sized muscular arteries, as measured by pulse wave velocity (PWV). The aim of this study was to examine the differential impact of NTG on regional stiffness, and arterial stiffness gradient as measured by the aortic-brachial PWV ratio (AB-PWV ratio) and aortic-femoral PWV ratio (AF-PWV ratio).Methods: In 93 subjects (age: 61 years, men: 67%, chronic kidney disease [CKD]: 41%), aortic, brachial, and femoral stiffnesses were determined by cf-PWV, carotid-radial (cr-PWV), and femoral-dorsalis pedis artery (fp-PWV) PWVs, respectively. The measurements were repeated 5 min after the sublingual administration of NTG (0.4 mg). The AB-PWV and AF-PWV ratios were obtained by dividing cf-PWV by cr-PWV or fp-PWV, respectively. The central pulse wave profile was determined by radial artery tonometry through the generalized transfer function.Results: At baseline, cf-PWV, cr-PWV, and fp-PWV were 12.12 ± 3.36, 9.51 ± 1.81, and 9.71 ± 1.89 m/s, respectively. After the administration of NTG, there was a significant reduction in cr-PWV of 0.86 ± 1.27 m/s (p < 0.001) and fp-PWV of 1.12 ± 1.74 m/s (p < 0.001), without any significant changes in cf-PWV (p = 0.928), leading to a significant increase in the AB-PWV ratio (1.30 ± 0.39 vs. 1.42 ± 0.46; p = 0.001) and AF-PWV ratio (1.38 ± 0.47 vs. 1.56 ± 0.53; p = 0.001). There was a significant correlation between changes in the AF-PWV ratio and changes in the timing of wave reflection (r = 0.289; p = 0.042) and the amplitude of the heart rate-adjusted augmented pressure (r = − 0.467; p < 0.001).Conclusion: This study shows that acute administration of NTG reduces PWV of muscular arteries (brachial and femoral) without modifying aortic PWV. This results in an unfavorable profile of AB-PWV and AF-PWV ratios, which could lead to higher pulse pressure transmission into the microcirculation.

Pulse wave velocity (PWV) is used to evaluate regional stiffness of large and medium-sized arteries. Here, we examine the feasibility and reliability of radial-digital PWV (RD-PWV) as a measure of regional stiffness of small conduit arteries and its response to changes in hydrostatic pressure. In 29 healthy subjects, we used Complior Analyse piezoelectric probes to record arterial pulse wave at the radial artery and the tip of the index. We determined transit time by second-derivative and intersecting tangents using the device-embedded algorithms and in-house MATLAB-based analyses of only reliable waves and by numerical simulation using a one-dimensional (1-D) arterial tree model coupled with a heart model. Second-derivative RD-PWV was 4.68 ± 1.18, 4.69 ± 1.21, and 4.32 ± 1.19 m/s for device-embedded, MATLAB-based, and numerical simulation analyses, respectively. Intersecting-tangent RD-PWV was 4.73 ± 1.20, 4.45 ± 1.08, and 4.50 ± 0.84 m/s for device-embedded, MATLAB-based, and numerical simulation analyses, respectively. Intersession coefficients of variation were 7.0% ± 4.9% and 3.2% ± 1.9% (

Purpose/Background/Objective: We have previously shown that restoration of kidney function through kidney transplantation (KTx) is associated with improved aortic stiffness. In this study, we aim to examine whether this change in aortic stiffness translates into improvement of radial artery systolic-diastolic pulse transit time. Methods: Before and three months after KTx, we obtained radial pressure waveforms using applanation tonometry, in a group of 61 patients with restored renal function (eGFR > 45 ml/min/1,73 m2). Radial waveforms were recorded over a 10 seconds period and ensemble-averaged (using in house-MATLAB program) to obtain a single waveform and then modelled using two Gaussian functions, was then determined as the transit time between the first systolic peak T1 and the early diastolic peak T2. Results: 61 patients (66% male, mean age: 48 ± 14 years, mean eGFR 3 months after Ktx: 66.0 ± 17.1) were assessed. After KTx, there was a significant reduction in central systolic (125,266 ± 21,848 to 108,994 ± 14,407, p < 0.001) and diastolic BP (84,718 11,679 to 74,092 9,774, p < 0.001), carotid-femoral PWV (11,444 2,626 to 10,235 1,890, p < 0.001) and carotid-radial PWV (9,350 1,485 to 8,831 1,291, p = 0,003). While T1 declined (0.184 [0.173–0.198] to 0.180 [0.168–0.194], p = 0.018), there were no significant changes in T2 (0.322 [0.295–0.360] to 0.318 [0.283–0.355], p = 0.169) and in dT1-2 (0.135 [0.119–0.161] to 0.134 [0.117–0.167], p = 0.457). Conclusions: Contrary to our expectation, three months after KTx, we did not observe a significant change in radial systolic-diastolic pulse transit time after kidney transplantation, despite an improvement of BP, aortic and brachial stiffness.

Aim: To validate a photoacoustic imaging (PAI) system, for the identification of intraplaque hemorrhage, comparing it with MRI and histology (gold standard). Methods: 25 patients with carotid stenosis >70% and clinical indication to tromboendoarterectomy were recruited. Angio-MRI for intraplaque hemorrhage assessment (Cube sequence) was performed. PAI clips (5 seconds, Frame rate 1000/sec, 3 to 15 per patient) were acquired. Each clip was scored for the presence of PAI signal by means of an integrated scoring system (semiquantitative, from 0 to 12). Semiquantitative grading scales were used to assess plaque histological features of hemorrhage and vulnerability. Results: 18 patients had no missing MRI, PAI and histology data and were included in this analysis. Mean age was 73 ± 8 years, 60% men, 80% Caucasians, 92% hypertensives, 60% with a previous stroke. At histology, only 3 plaques out of 21 showed no signs of intraplaque hemorrhage, 4 showed small hemorrhage, while 14 (67%) showed large hemorrhages. PAI score (best cut-off ≥ 4) correctly classified 14 out of 18 patients (Sensitivity = 73.3%, specificity = 100%, AUC = 0.867). MRI performance was substantially similar, with 12 patients correctly classified (sensitivity = 60%, specificity = 100%, AUC = 0.800), with a non-significant difference in AUC compared to PAI (p = 0.420). Conclusions: In this first in-vivo human study, PAI is able to identify histological intraplaque hemorrhage with an excellent specificity and acceptable sensitivity, equivalent to MRI. The very high specificity, with a low number of false positives, make PAI a good candidate for evaluation of plaques prior to surgery to i.e. reinforce the decision to perform surgery.

Pulse wave velocity (PWV) has been used to evaluate arterial stiffness of large arteries. Here, we examine the feasibility of radial-digital PWV (RD-PWV) as a measure of stiffness of smaller arteries, and its response to changes in local mean arterial pressure. In 29 healthy subjects, we used Complior probes to record arterial pulse wave at radial artery and tip of the index. To determine transit time, we used both second derivative and intersecting tangents of the entire recordings using the device-embedded algorithms, in house Matlab analyses of only reliable waves, and by numerical simulation using arterial tree model. In 15 subjects, we examine the response of RD-PWV to changes in local MAP by vertical displacement of the hand above and below the mid-axillary line. Using second derivative, RD-PWV were 4.68 ± 1.18, 4.69 ± 1.21, 4.32 ± 1.19 m/s respectively for device-embedded, Matlab-based and numerical simulation analyses, respectively. Using intersecting tangents RD-PWV were 4.73 ± 1.20, 4.45 ± 1.08, 4.50 ± 0.84 m/s, respectively for device-embedded, Matlab-based and numerical simulation analyses, respectively. The strongest correlation (r = 0.92) was seen between device-embedded and Matlab-based second derivatives. The intersession coefficients of variation were 7.0 ± 4.9% and 3.2 ± 1.9% (p = 0.04) for device-embedded and Matlab-based second derivative algorithms. We estimated that each increase of 10 mm Hg in local MAP by vertical displacement of the hand resulted in an increase in RD-PWV of 0.28 m/s. This study shows that RD-PWV can be used for the non-invasive assessment of stiffness of small-sized arteries.

IntroductionPatients with end-stage renal disease are at higher risk of cardiovascular morbidity and mortality, a risk mediated in part by increased aortic stiffness. Arterial stiffness is assessed at different anatomical locations (central elastic or peripheral muscular arteries) using a variety of mechanical biomarkers. However, little is known on the robustness of each of these mechanical biomarkers following a haemodynamic stress caused by a single haemodialysis (HD) session.Methods and analysisA systematic review has been designed and reported in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols. A targeted search strategy applicable in key databases (PubMed, Embase, the Cochrane Library, Web of Science and grey literature) is constructed to search articles and reviews from inception to 16 October 2020. Only articles of studies conducted with adults under chronic HD for kidney failure, with repeated measures of arterial stiffness metrics (pulse wave velocity, Augmentation Index, arterial distensibility or stiffness) following a before-and-after design surrounding a HD session will be selected. The screening process, data extraction and assessment of risk bias will be done by two independent pairs of reviewers. Meta-analysis will enable adjustments for potential confounders and subgroup analyses will be performed to discriminate changes in arterial stiffness metrics from elastic, muscular or global arterial territories.Ethics and disseminationThis study does not require ethical approval. Findings will be submitted for publication to relevant peer-reviewed journals and will be presented at profession-specific conferences.PROSPERO registration numberCRD42020213946.

Pulse waveform analyses have become established components of cardiovascular research. Recently several methods have been proposed as tools to measure aortic pulse wave velocity (aPWV). The carotid-femoral pulse wave velocity (cf-PWV), the current clinical gold standard method for the noninvasive assessment of aPWV, uses the carotid-to-femoral pulse transit time difference (cf-PTT) and an estimated path length to derive cf-PWV. Objective: The heart-ankle PWV (ha-PWV), brachial-ankle PWV (ba-PWV) and finger-toe (ft-PWV) are also methods presuming to approximate aPWV based on time delays between physiological cardiovascular signals at two locations (similar to heart-ankle PTT, ha-PTT; similar to brachial-ankle PTT, ba-PTT; similar to finger-toe PTT, ft-PTT) and a path length typically derived from the subject's height. To test the validity of these methods, we used a detailed 1D arterial network model (143 arterial segments) including the foot and hand circulation. Approach: The arterial tree dimensions and properties were taken from the literature and completed with data from patient scans. We calculated PTTs with all the methods mentioned above. The calculated PTTs were compared with the aortic PTT (aPTT), which is considered as the absolute reference method in this study. Main results: The correlation between methods and aPTT was good and significant, cf-PTT (R-2 = 0.97; P < 0.001; mean difference 5 +/- 2 ms), ha-PTT (R-2 = 0.96; P < 0.001; 150 +/- 23 ms), ba-PTT (R-2 = 0.96; P < 0.001; 70 +/- 13 ms) and ft-PTT (R-2 = 0.95; P < 0.001; 14 +/- 10 ms). Consequently, good correlation was also observed for the PWV values derived with the tested methods, but absolute values differed because of the different path lengths used. Significance: In conclusion, our computer model-based analyses demonstrate that for PWV methods based on peripheral signals, pulse transit time differences closely correlate with the aortic transit time, supporting the use of these methods in clinical practice.

BACKGROUND Carotid-femoral pulse wave velocity (PWV) (cf-PWV) is the gold standard for measuring aortic stiffness. Finger-toe PWV (ft-PWV) is a simpler noninvasive method for measuring arterial stiffness. Although the validity of the method has been previously assessed, its accuracy can be improved. ft-PWV is determined on the basis of a patented height chart for the distance and the pulse transit time (PTT) between the finger and the toe pulpar arteries signals (ft-PTT). METHOD The objective of the first study, performed in 66 patients, was to compare different algorithms (intersecting tangents, maximum of the second derivative, 10% threshold and cross-correlation) for determining the foot of the arterial pulse wave, thus the ft-PTT. The objective of the second study, performed in 101 patients, was to investigate different signal processing chains to improve the concordance of ft-PWV with the gold-standard cf-PWV. Finger-toe PWV (ft-PWV) was calculated using the four algorithms. RESULTS The best correlations relating ft-PWV and cf-PWV, and relating ft-PTT and carotid-femoral PTT were obtained with the maximum of the second derivative algorithm [PWV: r = 0.56, P

This paper aims to summarize and map contemporary views on some contentious aspects of arterial hemodynamics that have remained unresolved despite years of research. These were discussed during a workshop entitled Arterial hemodynamics: past, present and future held in London on June 14 and 15, 2016. To do this we formulated a list of potential consensus statements informed by discussion at the meeting in London and quantified the degree of agreement and invited comments from the participants of the workshop. Overall the responses and comments show a high measure of quantitative agreement with the various proposed 'consensus' statements. Taken together, these statements seem a useful basis for proceeding with a more detailed and comprehensive consensus document on the current understanding and approaches to analysis of the pulse waveform. Future efforts should be directed at identifying remaining areas of dispute and future topics for research. (C) 2017 Association for Research into Arterial Structure and Physiology. Published by Elsevier B.V. All rights reserved.

Background: Ankylosing spondylitis (AS) is an inflammatory autoimmune disease. AS is a prototype form of spondyloarthropathies (SpA). The precise ethology of AS has not been fully understood. But Inflammation has a critical role in the pathogenesis of the disease. Extra skeletal organs may also be affected by this disease and is also associated with an increase of cardiovascular risk. The effect of large arteries appears by a stiffness that can be an element of disease monitoring. Objective: The objective of this study was to evaluate the finger-toe Pulse Wave Velocity (ftPWV) in patients with AS. Methods: Finger-toe pulse wave velocity (ft-PWV) was measured by pOpmetre® allowed to explore arterial stiffness. Results: Demographic and clinical characteristics are presented in Table 1. Twenty-two patients with AS and 24 controls were included in our study, subjects with AS exhibited greater pSBP (p < 0.001), pDBP (p < 0.001), pPP (p < 0.001) and MBP (p < .001) compared to controls. Moreover, in the AS group we observed a higher ftPWV with a mean difference of 1.63 (p < 0.006, 95% CI of .50 to 2.7). No significant difference was observed in pPP. Conclusions: Individuals with ankylosing spondylitis showed increased ftPWV, central and peripheral blood pressure, this contributes to explain the higher risk of cardiovascular disease in this pathology. pOpmètre® is a no operator depended, simple and practical device, highlighted an increase in arterial stiffness in patients with AS by measuring the ft-PWV. It could play a role in this disease monitoring and in prediction of cardiovascular complications.

Purpose/Background/Objective: Cognitive decline is highly prevalent amongst end-stage renal disease (ESRD) patients and is accelerated upon initiation of hemodialysis (HD) [1]. ESRD increases aortic stiffness and blood flow pulsatility, which may damage small vessels of target organs like the brain [2]. In this pilot study, we aimed to evaluate the acute effect of HD on cerebral blood flow and its relation to arterial stiffness. Methods: Before, every hour during and after HD (T0–T4), we measured cerebral flow velocity (FV) using transcranial Doppler, blood pressure (BP) via digital finger cuff (Nexfin), cardiac activity using ECG and aortic pulse wave velocity (PWV) with Mobile-O-Graph. FV pulsatility index (PI) and transit times between ECG peak and the foot of both FV and BP waveforms (cerebral dT; digital dT) were computed using in house MATLAB-based analysis. Changes during HD were evaluated with Generalized Estimating Equation models adjusting for multiple comparisons in SPSS 26.0. Results: In eight participants aged 63 ± 17 y. old (4 diabetics, 3 women), peak FV decreased from baseline at T1 and T2 (−11.2 cm/s, p = 0.007; −12.2 cm/s, p < 0.001), PI decreased at T1 (0.81 to 0.77, p = 0.005), whilst minimum FV, mean BP and partial pressure of CO2 remained unchanged. Digital dT increased at T3 (0.19 to 0.22, p < 0.001) and cerebral dT increased throughout HD (T1–T4, p < 0.005), whereas aortic PWV did not change. Conclusions: During hemodialysis, cerebral and digital transit times increased, suggesting decreased stiffness of small peripheral vessels, without significant changes in aortic stiffness. Reduced stiffness of cerebral arteries may partially explain decreased cerebral flow pulsatility.

Background: Identification of intraplaque haemorrhage using magnetic resonance imaging (MRI) is expensive, technically demanding and sometimes uncomfortable. Photoacoustic imaging (PAI) is a new non-invasive technique combining multi-wavelength infrared laser light and ultrasound (US) imaging, able to discriminate blood and other components in the tissues. The measurement by the multi-spectral PAI is likely to be more applicable and therefore could be complementary to MRI. Objectives: Validate a portable multimodal and multi-wavelength PAI system, for the identification of intraplaque haemorrhage and compare with MRI. Materials and Methods: Patients underwent carotid MRI, and US. Photoacoustic acquisitions are performed and analysed a posteriori blindly of the status of the subjects and results of MRI/US. All patients gave written informed consent. Histology of plaque will be performed as gold standard. Results: We included 15 patients (71 ± 5 years old) with carotid stenosis grade of >70% (NASCET). PAI imaging was feasible in all patients. On raw images, only clutter signal can be observed. After adequate post processing, carotid wall with the intima media and plaques were clearly visible with PAI. PAI signal from the lumen can be observed, also strong PAI signals are visible inside the plaque in a reproducible manner. Correspondence between MRI and PAI will be analysed later. Conclusion: The use of the PAI technique for carotid plaque assessment is feasible, and PAI signal are observed inside the plaque. Further analyses are needed to assess the reflected PAI signal inside the plaque, for haemorrhage detection in the future.

Arterial stiffness may influence the contour of the peripheral pulse, suggesting that contour analysis of the digital volume pulse (DVP) might be used to estimate peripheral blood pressure and a stiffness index (SI). The objective is to establish a transfer function that estimates the peripheral blood pressure using the PPG pulse, calibrated with a brachial pressure cuff, and then to deduce a stiffness index using the established parameters. We positioned the photodiode sensor on the finger. Brachial blood pressure measurement was performed with a cuff adapted to the arm circumference and an oscillometric device (Omron M10 -IT). Pulse wave velocity (PWV) measurements were performed with the pOpmètre® system. DVP waveforms were recorded over a 10 s period and ensemble-averaged to obtain a single waveform from which DT (DVP) was determined as the time between the first systolic peak and the early diastolic peak/inflection point in the waveform. The SI is the ratio of the subject’s height to the DT (DVP). 69 subjects were included: 24 healthy subjects and 45 patients with essential hypertension. The correlation between the estimated peripheral diastolic pressure and the brachial one was good and significant (r2 = 0.51; p < 0.001). A better correlation was found in terms of peripheral systolic pressure (r2 = 0.56; p < 0.001). The correlation between the SI and the ft-PWV was significant (r = 0.5; p < 0.001) classifying the estimation as good agreement. The estimation of the peripheral blood pressure and a stiffness index with the PPG signal qualifies as good agreement with the reference technique.

Recently several methods have been proposed as tools to measure aortic pulse wave velocity (aPWV). The carotid-femoral pulse wave velocity (cf-PWV), the current clinical gold standard method for the noninvasive assessment of aPWV, uses the carotid–femoral pulse transit time (cf-PTT) to derive cf-PWV. The heart-ankle PWV (ha-PWV), brachial-ankle PWV (ba-PWV) and finger-toe (ft-PWV) are also methods presuming to approximate aPWV based on time delays between physiological signals at two locations (∼heart-ankle PTT, ha-PTT; ∼brachial-ankle PTT, ba-PTT; ∼finger-toe PTT, ft-PTT). To test the validity of these methods, we used a 1D arterial network model (143 segments) including the foot and hand circulation. The arterial tree dimensions and properties were taken from the literature and completed with CT-scans data. We calculated PTT’s with all the methods above. The calculated PTT’s were compared with the aortic PTT (aPTT), considered as the absolute reference method in this study. The correlation between methods and aPTT was good and significant, cf-PTT (R2 = 0.97; P < 0.001; mean difference 5±2 ms), ha- PTT (R2 = 0.96; P < 0.001; 150±23 ms), ba-PTT (R2 = 0.96; P < 0.001; 70±13 ms) and ft-PTT (R2 = 0.95; P < 0.001; 14±10 ms). Consequently, good correlation was also observed for the PWV values derived with the tested methods, but absolute values differed because of different path lengths used. In conclusion, our computer model based analyses demonstrate that for PWV methods based on peripheral signals, PTT’s closely correlate with the aPTT, supporting the use of these methods in clinical practice.

Arterial Stiffness (AS) is of great interest in the prediction and the management of cardiovascular events. The evaluation of AS using 2 points of measurement of the pulse wave velocity was of considerable increasing interest in the early 21st century. This paper aimed at focusing on the modeling of different measurement methods. Several measurement techniques are presented. A review of the bibliography and an explanation of the link between cardiovascular events and arterial stiffness are summarized. Novel apparatus that were developed for determining arterial stiffness claimed superiority over pioneering methods either through greater simplicity of use, better repeatability, or a more pertinent arterial pathway.

Background: Large artery stiffness is recognized as a strong, independent marker of cardiovascular risk, mainly through aortic pulse wave velocity (PWV). pOpmètre® is a new non-invasive method, which estimates aortic PWV through finger-toe (FT) wave analysis. In a previous study, Alivon et al. have shown an acceptable correlation (r2 = 0.43 for PWV) between pOpmètre® and the reference method Sphygmocor. However this study led to the necessity to optimize the algorithm and the procedures because of the presence of several outliers involving mainly obese and elderly subjects. Materials and Methods: The pOpmètre® has 2 photodiodes sensors, positioned on the finger and on the toe. A particular attention was drawn on positioning of the toe sensor so that the pulp was in contact with the photodiode. Different signal processing chains were applied and no cut-off value was used for pulse height. Applanation tonometry was performed for CF PWV measurements. Results: 45 subjects were included: 18 healthy subjects and 27 patients with essential hypertension aged 32± 7 years and 58 ±18 years respectively. The correlation between FT PWV and CF PWV was good and significant (r2 = 0.77 p

Aim: The sickle cell trait (SCT) is the heterozygous and benign form of the homozygous SC disease (SCD). Although SCT exhibits hemorheological disturbances and increased oxidative stress, its exact role in the development of cardiovascular disease in SCT remains to be determined. The present study sought to determine the arterial stiffness, an independent marker of sub-clinical atherosclerosis, between SCT and trained SCD. Materials and methods: Twelve SCT (43±11yrs, 6 males) were compared to 14 trained younger SCD (29±9yrs, 2 males) and 26 controls with normal hemoglobin (36±11rs, 13 males). Aortic (AoStiff-Physioflow) and peripheral arterial stiffness (pOpmetre-Axelife-France) assessed by foot-to-toe pulse wave velocity (ft-PWV), blood pressure (BP) and Framingham-Laurier cardiovascular risk score (CVR in %) were measured in each group. Results: SCT exhibited stiffer arteries (Aostiff: 5.8±0.71 m/s, ft-PWV: 10.9±6.2 m/s) than controls (Aostiff = 4.8±0.5 m/s, ft-PWV: 7.7±2.6 m/s) and SCD (Aostiff = 4.9±0.6 m/s, p=0.001, ft-PWV: 6.1±0.9 m/s p=0.003). SCT were older (p=0.001) than controls and SCD. The systolic and mean AP were lower in SCD than controls and SCT. In the whole group, the CVR score was the major contributor to PerStiff (r2=0.402, p

Analysis of the contour of the peripheral pulse to assess arterial properties was first described in the nineteenth century. The reference technique to estimate central blood pressure (CBP), non-invasively, is by analyzing the radial pressure pulse acquired using a tonometer and then to establish a transfer function relating the radial pressure to the CBP. An alternative technique utilizes a volume pulse. This may conveniently be acquired optically from a finger. This technique deserves further consideration because of its simplicity and ease of use. The objective is to establish a transfer function estimating the central blood pressure (CBP), calibrated with a brachial pressure cuff and using the parameters obtained with the contour analysis of the photoplethysmographic pulse measured at the finger with the pOpmetre® system. We positioned the photodiode sensor on the finger, insuring that the sensor's lens is in contact with the finger. Brachial blood pressure measurements was performed with a standard cuff. The central blood pressure values measured with Sphygmocor used as the reference values. Multiple regression analysis was done to establish the transfer functions. Sixty-nine subjects were included: 24 healthy subjects and 45 patients with essential hypertension aged 33 ± 8 years and 59 ± 17 years respectively. The correlation between the estimated central systolic pressure (CSP) and the reference one, was good and significant (r2 = 0.91; P

Background Arterial pulse wave velocity and pulse waveform analysis have become an established component of cardiovascular research. As validation and assessment of devices is not always trivial in an in vivo setting, arterial network computer models may be useful for that purpose. It is, however, mandatory that the model includes sufficient detail, especially when analysing peripheral waveforms. Purpose To extend the existing 1D arterial network model (103 segments) of Reymond et al. to a more detailed model (143 segments) including the foot and hand circulation (radial and tibial arteries). The goal is to (i) extend the existing 1D arterial network model (103 segments) of Reymond et al. to a more detailed model (143 segments) including the foot and hand circulation (Radial and Tibial arteries); (ii) use the extending model as testing tool for pOpmetre® (finger–toe pulse wave velocity). Method The arterial tree dimensions and properties were taken from the literature and completed with data from patient scans. The model solves the one-dimensional form of the Navier-Stokes equations over each arterial segment. A non-linear viscoelastic constitutive law for the arterial wall was considered. Result Comparison of simulations with & without detailed hand and foot circulation demonstrate important differences in waveform morphology in the distal beds. The completed model predicts pressure and flow waves in the hand and foot arteries which are in good qualitative agreement with the published in-vivo measurements. The agreement is especially good for the shape and wave details of the flow wave, where all features are reproduced in a rather faithful manner. The correlation between ftPWV and aPWV was good and significant (R2 = 0.95). The Bland and Altman analysis, mean difference was 0.4 m/s, classifying the ftPWV as good agreement with reference method. Conclusion The extended model yields realistic pressure and flow waveforms in arteries of the hand and the foot.

Objective: Pulse Wave Velocity (PWV) can be measured between different sites. Here we used two different aspects to assess the PWV; the standard method Carotid-Femoral (CF) Sphygmocor (AtCorMedical – Australia) and the pOpmetre ® (Axelife SAS – France) which uses the Finger to Toe (FT) signals. The aim of this study was to evaluate the agreement between FT-PWV and CF-PWV and to test the robustness of pOpmetre ® build-in algorithm. Design and method: CF and FT PWV was measured in 150 subjects. MatLab was used to calculate FT-PWV from pOpmetre ® waveforms using four methods: maximum of second derivative (used by pOpmetre ®), intersecting tangents, 10% threshold and the cross correlation method. Results: Using built in algorithms, the comparisons of the PWVs and transit times showed a good agreement between the two methods. FT-PWV correlated with CF-PWV (r2=0.51; p