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En la presente investigación realizada en Cuenca con tema valoración morfológica eritrocitaria en caballos aparentemente sanos en condiciones de altitud, se dieron estos resultados: equinocitos (1,41), cuerpos de howell-jolly (0,07) en machos. En hembras, equinocitos (1,10) cuerpos de howell-jolly (0,09) siendo estos los más relevantes. In the present investigation carried out in Cuenca with the theme of erythrocyte morphological assessment in apparently healthy horses under high altitude conditions, the following results were obtained: echinocytes (1.41), Howell-jolly bodies (0.07) in males. In females, echinocytes (1.10) and howell-jolly bodies (0.09), these being the most relevant.

La presente investigación se determinó valores referenciales de alteraciones e inclusiones eritrocitarias en vacas Holstein Friesian y se pudo establecer que factores como la altitud, y artefactos, influyen en la presentación de estas formas eritrocitarias. The present investigation determined reference values of erythrocyte alterations and inclusions in Holstein Friesian cows and it was possible to establish that factors such as altitude, and artifacts, influence the presentation of these erythrocyte forms.

El presente trabajo experimental tuvo como objetivo determinar valores referenciales de reticulocitos y leucocitos en caninos aparentemente sanos en condiciones de altitud, en el cantón Cuenca, provincia del Azuay. Para obtener valores reales de la zona y poder mejorar diagnósticos y tratamientos en el área de Medicina Veterinaria. The objective of this experimental work was to determine reference values of reticulocytes and leukocytes in apparently healthy canines under high altitude conditions, in Cuenca canton, Azuay province. To obtain real values of the area and to be able to improve diagnoses and treatments in the area of Veterinary Medicine.

El presente trabajo se elaboró para valorar hemograma y química sanguínea, niveles de ácido láctico y cortisol en equinos con antecedentes de hiperparatiroidismo secundario nutricional, el lugar ya contaba con una previa incidencia del mismo. El estudio se realizó con el objetivo de medir cuantitativamente los parámetros anteriormente descritos y evaluar los resultados de este estudio. This study was developed to assess blood count and blood chemistry, lactic acid and cortisol levels in horses with a history of nutritional secondary hyperparathyroidism, the place already had a previous incidence of it. The study was carried out with the objective of quantitatively measuring the parameters described above and evaluating the results of this study.

La presente investigación se enfocó en establecer valores de referencia en cerdos aparentemente sanos en condiciones de altitud, donde se analizó las formas leucocitarias, conteo de reticulocitos y niveles de hematocrito, se trabajó con 120 muestras, 60 hembras y 60 machos. Mediante el análisis estadístico se obtuvieron valores con diferencias significativas con respecto a los valores referenciales. The present investigation focused on establishing reference values in apparently healthy pigs in high altitude conditions, where leukocyte forms, reticulocyte count and hematocrit levels were analyzed, working with 120 samples, 60 females and 60 males. Through statistical analysis, values with significant differences were obtained with respect to the reference values.

El estudio tuvo como objetivo establecer valores de referencia propios en cerdos, donde se analizó 16 tipos de alteraciones morfológicas eritrocitarias, esto en extensiones de sangre periférica en cerdos aparentemente sanos en condiciones de altitud. Se realizó el análisis estadístico, obteniendo así valores con una diferencia significativa respecto a los valores referenciales. The study aimed to establish own reference values in pigs, where 16 types of erythrocyte morphological alterations were analyzed, this in peripheral blood smears in apparently healthy pigs under altitude conditions. Statistical analysis was performed, thus obtaining values with a significant difference with respect to the reference values.

Este artículo se encuentra disponible en la siguiente URL: https://actavetscand.biomedcentral.com/track/pdf/10.1186/s13028-021-00573-3.pdf Myeloid disorders are conditions being characterized by abnormal proliferation and development of myeloid lineage including granulocytes (neutrophils, eosinophils and basophils), monocytes, erythroids, and megakaryocytes precursor cells. Myeloid leukemia, based on clinical presentation and proliferative rate of neoplastic cells, is divided into acute (AML) and myeloproliferative neoplasms (MPN). The most commonly myeloid leukemia reported in horses are AML-M4 (myelomonocytic) and AML-M5 (monocytic). Isolated cases of AML-M6B (acute erythroid leukemia), and chronic granulocytic leukemia have also been reported. Additionally, bone marrow disorders with dysplastic alterations and ineffective hematopoiesis affecting single or multiple cell lineages or myelodysplastic diseases (MDS), have also been reported in horses. MDSs have increased myeloblasts numbers in blood or bone marrow, although less than 20%, which is the minimum level required for diagnosis of AML. This review performed a detailed description of the current state of knowlegde of the myeloproliferative disorders in horses following the criteria established by the World Health Organization.

Ozone (O3) therapy has been used for medical procedures for centuries; however, there are no extensive studies on its utilization in horses. This study aimed to evaluate the application of transrectal O3 on horses by physical and laboratorial evaluation, and production of reactive oxygen species (ROS). Sixteen healthy horses were separated in two groups: a control group (CG) and a group treated with O3 (TG). The TG animals received 1L of an oxygen and O3 mixture transrectally. The initial dose was 10µg/ml for the first two applications, 15μg/ml for the following two applications, and 20μg/ml for the next six applications. The CG animals received 1L of oxygen transrectally. In TG animals no variations in the physical examination were detected; furthermore, TG animals did not exhibit changes in biochemical evaluation results, fibrinogen concentrations, or ROS production. TG animals had increased red blood cell counts, hemoglobin concentrations, and packet cell volume values in comparison to the baseline and CG values. We could infer that O3 affected the red blood cell counts and improved rhetological properties of the blood. The transrectal application of O3 in horses is safe and can indirectly improve the oxygenation and metabolism of tissues.

En la presente investigación se determinó valores de referencia en hemograma y química sanguínea en 100 vacas raza Holstein friesian en producción en condiciones de altitud en el cantón Girón. Ciertos valores obtenidos tuvieron una diferencia significativa con respecto a los valores referenciales, atribuidos más a la alimentación y estilo de vida del animal que a la altura. In the present investigation, reference values were determined using hemogram and blood chemistry in 100 Holstein Friesian breed cows in high altitude conditions in the city of Girón. Certain obtained values had a significant difference with respect to the reference values, attributed more to the diet and lifestyle of the animal than to the height.

Al determinar los valores de referencia de Serie Blanca y Reticulocitos en felinos de la ciudad de Cuenca, con su análisis respectivo, se obtuvo resultados favorables al factor altitud, por no presentar ninguna alteración en sus variables estudiadas comparándolo a altitudes similares, a diferencia de los Linfocitos que se alteró en referencia con una altitud menor. When determining the reference values of White Series and Reticulocytes in felines from the city of Cuenca, with their respective analysis, favorable results were obtained for the altitude factor, as they did not present any alteration in their studied variables when compared to similar altitudes, unlike the Lymphocytes that were altered in reference to a lower altitude.

En la presente investigación se determinó valores referenciales tanto de alteraciones e inclusiones eritrocitarias en gatos de la ciudad de Cuenca y se pudo establecer que factores como la altitud, especie y artefactos, influyen en la presentación de estas formas eritrocitarias, estableciendo variables patológicas, fisiológicas y laboratoriales. In the present investigation, reference values were determined for both erythrocyte alterations and inclusions in cats in the city of Cuenca and it was possible to establish that factors such as altitude, species and artifacts influence the presentation of these erythrocyte forms, establishing pathological, physiological and laboratories.

En la presente investigación se determinó valores de referencia en hemograma y química sanguínea en 100 gatos machos en condiciones de altitud en la ciudad de Cuenca. Ciertos valores obtenidos tuvieron una diferencia significativa con respecto a los valores referenciales, atribuidos más a la alimentación y estilo de vida del animal que a la altura. In the present investigation, reference values were determined in hemogram and blood chemistry in 100 male cats in high altitude conditions in the city of Cuenca. Certain values obtained had a significant difference with respect to the reference values, attributed more to the diet and lifestyle of the animal than to the height.

El presente trabajo experimental tuvo como objetivo determinar valores referenciales de reticulocitos y leucocitos en equinos (Equus caballus) aparentemente sanos en condiciones de altitud, en el Cantón Cuenca, provincia del Azuay. Para obtener valores reales de la zona y poder mejorar diagnósticos y tratamientos en el área de Medicina Veterinaria. The objective of this experimental work was to determine the reference values of reticulocytes and leukocytes in apparently healthy equines (Equus caballus) in altitude conditions, at the city of Cuenca, located in the province of Azuay. To obtain real values of the area and to be able to improve diagnoses and treatments in Veterinary Medicine.

La presente investigación se determinó los valores de referencia en hemograma y química sanguínea de 100 gatos hembra en condiciones de altura, para el análisis de datos se utilizó el software Minitab17. Los parámetros estudiados tuvieron diferencia significativa con los valores de referencia citados, obteniendo un rango más amplio que la bibliografía. The present investigation determined the references values in hemogram and blood chemistry of 100 female cats in high altitude conditions, for data analysis the Minitab17 software was used. The parameters studied had a significant difference with the reference values, obtaining a wider range than the bibliography.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) A tecnologia de bioflocos (BFT) é um sistema de produção intensiva com baixa renovação de água; contudo, o acúmulo de compostos nitrogenados e matéria orgânica pode vir a ser problemático se esse não for tratado com eficiência. Uma das técnicas de tratamento destes compostos é a inclusão de fontes de carboidrato orgânico no sistema. Portanto, com o presente estudo objetivou-se avaliar a influência do uso de fontes de carbono no sistema de bioflocos sobre os parâmetros de qualidade da água, e de desempenho e homeostase de juvenis de tilápias do Nilo. Foram testadas quatro diferentes fontes de carbono (bagaço de cana, milho, milho desintegrado com palha e sabugo e polpa cítrica) em comparação ao açúcar, que foi utilizado como fonte padrão controle. Os tanques foram preparados utilizando 30% de bioflocos já maturados e 70% de água clara. Um total de 400 juvenis de tilápias do Nilo (45,4 ± 3,66 g) foram distribuídos aleatoriamente em 20 tanques experimentais de 160 L de volume útil em uma densidade de 20 peixes por tanque, sendo quatro repetições por fonte de carbono. Foram analisados os parâmetros físico-químicos e compostos nitrogenados da água. Quando a amônia ultrapassava o nível de 1 mg.L -1 , as fontes de carbono eram adicionadas na proporção de 6:1 (C:N). Ao final do período experimental foi realizada a avaliação bromatológica das fontes carbonáceas, dos bioflocos e dos filés de tilápia, do tamanho dos flocos formados e do desempenho produtivo dos peixes. Também foram realizadas análises do eritrograma, do leucograma, do trombograma e da bioquímica plasmática do sangue, além da histomorfometria de brânquias. As fontes de carbonos testadas não influenciaram a qualidade da água do BFT de forma a prejudicar os peixes, não sendo observadas diferenças (p>0,05) para as variáveis temperatura (medianas de 25,9 a 27,4°C) e alcalinidade (mínimo 5,00 e máximo 215,00 mg.L -1 CaCO 3 ). O oxigênio dissolvido (4,05 a 5,63 mg.L -1 ), pH (5,7 a 5,9) e a salinidade (2,29 a 2,45 g.L -1 ) apresentaram diferenças (p0.05) in the variables temperature (medians from 25.9 to 27.4°C) and alkalinity (minimum 5.00 and maximum 215.00 mg.L -1 CaCO 3 ). Dissolved oxygen (4.05 to 5.63 mg.L -1 ), pH (5.7 to 5.9) and salinity (2.29 to 2.45 g.L - ) showed differences (p

La presente investigación consiste en establecer valores de referencia en hemograma y química sanguínea en porcinos machos a una altitud de 2595 m.s.n.m., se trabajó con 80 muestras sanguíneas y criados en sistemas de producción caseros y semi-tecnificado. The present investigation consists of establishing reference values in hemogram and blood chemistry in male pigs at an altitude of 2595 m.s.n.m., working with 80 blood samples and raised in homemade and semi-technical production systems.

En la presente investigación se empleó el método de extendidos sanguíneos, teñidos mediante la técnica de Diff-Quick, se utilizó 100 muestras experimentales, en un sistema de producción a pie. La investigación se enfocó en la visualización y análisis de la morfología eritrocitaria, asociación con enfermedad, descarto o normalidad dentro de la fisiología animal. In the present investigation the method of blood smears was used, stained by the Diff-Quick technique, 100 experimental samples were used, in an animal production system. The research focused on the visualization and analysis of erythrocyte morphology, association with disease, ruling out or normality within animal physiology.

En la presente investigación se empleó el frotis sanguíneo como técnica conjuntamente a la tinción diff quik. Se utilizaron 200 unidades experimentales., la investigación tuvo como fin identificar y clasificar las 16 alteraciones eritrocitarias e inclusiones en condiciones de altitud en bovinos de raza Holstein observadas en el microscopio trinocular. The present investigation used the blood smear as a test and the diff quik staining. 200 experimental units were used. The purpose of the research was to identify and classify the 16 erythrocyte alterations and inclusions under altitude conditions in Holstein cattle observed in the trinocular microscope. The data obtained may vary slightly due to the conditions of altitude, race, sex, age, diet, and exploitation system.

El trabajo experimental consiste en establecer valores de referencia de reticulocitos y leucograma (granulocitos, monocitos y linfocitos) en pollos de engorde cobb 500 a una altitud de 2260 m.s.n.m., se trabajó con 200 muestras sanguíneas, 100 de machos y 100 de hembras clínicamente evaluados y criados dentro de un sistema de producción controlado. Experimental work is to establish reticulocyte and leukogram reference values (granulocytes, monocytes and lymphocytes) in broiler chickens cobb 500 at an altitude of 2260 m.s.n.m., 200 blood samples, 100 male samples and 100 females clinically evaluated and bred within a controlled production system.

El trabajo experimental consiste en establecer valores referenciales de hemograma y química sanguínea en pollos machos de engorde cobb 500 a 2260 m.s.n.m., se establecieron valores de referencia de 14 parámetros de hemograma y 16 de química sanguínea a partir de 100 muestras sanguíneas de pacientes criados dentro de un sistema de producción controlado. Experimental work is to establish blood count and blood chemistry reference values in male broiler chickens cobb 500 to 2260 m.s.n.m., reference values of 14 blood count parameters were set and 16 blood chemistry from 100 blood samples from patients raised within a controlled production system.

Antioxidant rich foods have been widely used as defenders of the body against excessive oxidative damage and preventing different chronic diseases among them, the Diabetes. In the food industry, the synthetic antioxidants have been widely used to control lipid oxidation and the development of unpleasant flavors. However, the toxic and carcinogenic effects derived from the consumption of some synthetic antioxidants has led many researchers to investigate the use of natural antioxidants made from edible plants. Plant polyphenols are beneficial to human health, exerting various biological effects. Hydroxycinnamic acids are the main category of phenolic acids that can bring important benefits to human health. They are widely distributed in plants and most of their pharmacological properties are a result of their antioxidant action. Moreover, recent studies suggest that derivatives of hydroxycinnamic acid, have antidiabetic properties through several mechanism and without causing side effects. They could represent a new class of antidiabetic agents for the future. This review will address the role of oxidative stress in the pathogenesis of diabetes as well as discuss current knowledge of the biological effects of hydroxycinnamic acid derivatives on the control and prevention of diabetes. Los alimentos ricos en antioxidantes han sido ampliamente utilizados como defensores del organismo contra el daño oxidativo excesivo y previniendo diferentes enfermedades crónicas entre ellas, la Diabetes. En la industria alimentaria, los antioxidantes sintéticos han sido ampliamente utilizados para controlar la oxidación de los lípidos y el desarrollo de sabores desagradables. Sin embargo, los efectos tóxicos y cancerígenos derivados del el consumo de algunos antioxidantes sintéticos ha llevado a muchos investigadores a investigar el uso de antioxidantes naturales elaborados de plantas comestibles. Los polifenoles vegetales son beneficiosos para la salud humana, ya que ejercen diversos efectos biológicos. Los ácidos hidroxicinámicos son la principal categoría de ácidos fenólicos que pueden aportar importantes beneficios a la salud humana. Están ampliamente distribuidos en plantas y la mayoría de sus propiedades farmacológicas se deben a su acción antioxidante. Además, estudios recientes sugieren que los derivados del ácido hidroxicinámico tienen propiedades antidiabéticas a través de varios mecanismos y sin causar efectos secundarios. efectos secundarios. Podrían representar una nueva clase de agentes antidiabéticos para el futuro. Esta revisión abordará el papel del estrés oxidativo oxidativo en la patogénesis de la diabetes, así como el conocimiento actual de los efectos biológicos de los derivados del ácido hidroxicinámico en el control y la prevención de la diabetes. Escuela de Medicina Veterinaria

Background: Peripartum is a challenging phase for the health of cows. Objectives: This study analysed the haematological profile of Holstein cows during peripartum. Method: Blood samples were collected on days 18, 12, 8, 5 and 2 before calving, at parturition, and on days 1, 7, 14, 21, 30, 45 and 60 postpartum. Analyses of red blood cell (RBC) count, haemoglobin concentration, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and RBC distribution width were performed; body condition score (BCS) and milk yield were evaluated. Results: Red blood cell (the highest value was 6.10 × 10 12 /L at parturition and the lowest recorded value was 5.03 × 10 12 /L 21 days after parturition), haemoglobin and haematocrit (the highest values were 10.48 g/dL and 33.47% at parturition, respectively and the lowest values were 8.28 g/dL and 26.13% on day 30 after parturition, respectively); BCS (the highest and the lowest values were 3.50 points and 2.73 points on days 18 before parturition and 45 after parturition, respectively) and milk production (the lowest and the highest values were 21.48 L and 27.02 L on days 7 and 45 after parturition, respectively) were significantly different ( p < 0.05) during the peripartum period. Of the total cows ( n = 48), 41.7% had RBC, haemoglobin and haematocrit below the reference intervals during at least one collection point during the postpartum period. Conclusion: This study demonstrated that dairy cows included in this investigation suffered alterations in select haematological variables during the postpartum period.

Este artículo se encuentra disponible en la siguiente URL: https://ijvr.shirazu.ac.ir/article_5399_3cc0ca08dda95c802dd9ae406f6b464c.pdf The process of fibrin clot formation is a series of complex and well-regulated reactions involving blood vessels, platelets, procoagulant plasma proteins, natural inhibitors, and fibrinolytic enzymes. Vasculitis can be caused by a variety of different agents as bacteria, viruses, protozoal, rickettsial organisms, toxic, drugs, medications, and neoplasms. The most common cause of vasculitis is the purpura hemorrhagica, which is associated with exposure to Streptococcus equi ssp. equi or less commonly, equine influenza. Deficiencies or defects of the hemostatic components may result in bleeding and/or thrombosis. Inherited alterations of primary hemostasis (von Willebrand disease: vWD and Glanzmann’s thrombasthenia: GT) and of secondary hemostasis (hemophilia A and prekallikrein: PK deficiency) are scarcely reported in equine clinic. On the contrary, acquired alterations of primary and secondary hemostasis are commonly found. They include thrombocytopenia, platelet dysfunction due to the administration of some drugs and targeted antiplatelet agents, decreased factor synthesis (liver disease or deficiency of vitamin K), release of inactive factors, inhibition of factor activity, or excessive consumption and depletion of factors (platelets, coagulation factors, and anticoagulants factors as antithrombin (AT) and protein C). Disseminated intravascular coagulation (DIC) is the most common and complex hemostatic disorder in horses and appears to be associated with sepsis, inflammatory and ischemic gastrointestinal tract disorders and other systemic severe diseases. These alterations are commonly found in patients in intensive care units.

La presente investigación se tiene como objetivo principal la determinación valores referenciales en hemograma y química sanguínea en bovinos Holstein machos aparentemente sanos en condiciones de altitud, obteniendo100 muestras sanguíneas de pacientes que pertenecen a varias haciendas del cantón Cuenca, situadas a una altitud de 2500-3000 msnm. The main objective of this research is to determine baseline values in blood count and blood chemistry in apparently healthy male Holstein cattle in altitude conditions, obtaining 100 blood samples from patients who belong to several farms in the canton Cuenca, located at an altitude of 2500-3000 meters above sea level.

La presente investigación consiste en determinar valores referenciales en parámetros sanguíneos en bovinos hembra de raza Holstein a nivel de altura, las muestras sanguíneas fueron tomadas de 100 animales de predios pertenecientes al cantón Cuenca, las cuales nos sirve para obtener valores propios de la zona y así poder aportar a un diagnóstico más certero del paciente. The present investigation, the objective was to determine reference values in blood count and blood chemistry in female bovines of the Holstein breed at altitude conditions, the blood samples were taken from 100 farm animals belonging to the Cuenca canton, which helps us obtain values of the area and thus be able to contribute to a more accurate diagnosis of the patient.

El documento consiste en la determinación de la morfología eritrocitaria y anemias causadas por parasitismo gastrointestinal en caninos, con el fin de establecer datos correspondientes al medio, que servirá de base para diagnóstico y tratamiento de alteraciones en el componente sanguíneo. The document consists in the determination of erythrocyte morphology and anemias caused by gastrointestinal parasitism in dogs, in order to establish data in the medium, which will serve as a basis for diagnosis and treatment of alterations in the blood component.

CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Outra Agência In this study we evaluated the oral toxicity of leaf extracts of Annona crassiflora of for mice and the blood and parasitological parameters of lambs experimentally infected with Haemonchus contortus and treated with leaves of this plant. The highest dose of AE (aqueous extract) administered to mice (203.0 mg/kg bw) was well tolerated, suggesting low toxicity. At necropsy, macroscopic examination revealed no abnormalities of the evaluated viscera. Lambs infected with the nematode were divided one group treated with leaf powder and a control group that did not treat. Split-plot design analysis was performed where the treatments were defined as plots and three periods of collection were defined as subplots. Similar performances to weight gain were observed among the lamb groups. The oral administration of leaf powder at 2.75g /Kg bw did not alter the physiological blood parameters in comparison to untreated lambs; however, this dose was not efficient to fecal egg reduction. We consider that other formulations and administration protocols should be evaluated to promote an effective alternative control using the leaves of this plant. Neste estudo avaliou-se a toxicidade oral de extratos de folhas de Annona crassifloraem camundongos e os parâmetros parasitológicos e sanguíneos de cordeiros experimentalmente infectados com Haemonchus contortuse tratados com folhas dessa planta. A dose mais elevadado extrato aquoso (EA), administrada aos camundongos (203,0 mg / kg de peso corporal (pc)) foi bem tolerada, sugerindo baixa toxicidade. Na necropsia, o exame macroscópico não revelou anormalidades nas vísceras avaliadas. Cordeiros infectados com o nematódeo foram divididos em um grupo tratado com pó das folhas e um grupo controle não tratado. A análise do delineamento foi em parcelas subdivididas, os tratamentos foram definidos como parcelas e três períodos de coleta como subparcelas. O ganho de peso médio similar entre os grupos de borregos avaliados durante o período avaliado. A administração oral do pó das a 2,75g / kg pc não alterou os parâmetros fisiológicos do sangue em comparação aos cordeiros não tratados; no entanto, essa dose não foi eficiente para a redução fecal de ovos. Outras formulações e protocolos de administração devem ser avaliados para promover um controle alternativo e eficaz usando as folhas dessa planta.

The aim of this study was to evaluate the hematological, biochemical, and blood gas alterations of goat whole blood stored in different blood bags. Seven male, adult, crossbreed goats were used, weighing 62±1.8 kg. Nine hundred milliliters of whole blood from each animal was collected and stored in blood bags (450 ml in each), CPDA-1 (citrate phosphate dextrose-adenine) and CPD/SAG-M (citrate phosphate dextrose with saline-glucose-mannitol) as additive solutions, and kept refrigerated (2-4 ºC) for 42 days. Blood samples were collected from the plastic bags at baseline (T0) and after seven, 14, 21,28, 35, and 42 days for hematological, biochemical, blood gas, and microbiological evaluations. Free hemoglobin, degree of hemolysis, lactate, and pO2were increased in both bags, whereas hydrogen potential (pH) and the total hemoglobin concentration decreased overtime(P

CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior This study aimed to evaluate the hematological profile, hepatic function, and histopathology of mixed-sex broilers fed rations supplemented with microencapsulated essential oils from Cymbopogon flexuosus (lemon grass) and Lippia rotundifolia (chá-de-pedestre). One hundred and fifty Cobb chicks were housed in cages from 1 to 42 days of age in a completely randomized design, with six replicates with five chickens in each of five treatments (150 total chicks): basal diet without antibiotic growth promoter (negative control), diet with enramicina and salinomycin (positive control), diet with lemon grass essential oil, diet with L. rotundifolia essential oil, and diet with a mixture of lemon grass and L. rotundifolia essential oils. Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were significantly lower in untreated broilers (negative control group). Gender was not associated with erythrogram values, but aspartate aminotransferase activity (AST) was higher in females. Gamma-glutamyl transferase (GGT) was higher in chicks in the group that received rations with lemon grass oil. Broilers that received L. rotundifolia oil developed more hepatic lesions, although no effect of sex was observed related to the lesion score. Biliary hyperplasia and fibroplasias were observed in all groups, with higher histopathology scores in broilers that received diets containing L. rotundifolia oil. Mixed-sex broilers fed rations supplemented with lemon grass and L. rotundifolia essential oils have normal complete blood counts and unspecific hepatic lesions and are characterized by lipidosis, hyperplasia of the bile ducts, and fibroplasia.

Fundação de Amparo à Pesquisa do Estado de Minas Gerais O período de transição em éguas compreende o final da gestação, parto e início da lactação. Nesta fase as adaptações no organismo da égua são intensas e estão associadas com um estado metabólico alterado quando comparado com o período não-gestacional. O presente estudo teve como objetivo avaliar os efeitos do período de transição sobre parâmetros clínicos e laboratoriais em éguas da raça Mangalarga Marchador. Foram utilizadas 48 éguas, distribuídas em dois grupos: O grupo em manutenção (GM), formado por éguas não prenhes, e um grupo experimental, chamado de grupo de transição (GT), composto por éguas gestantes e que após o parto passaram a ser lactantes. As avaliações foram realizadas nos seguintes tempos: T-60 (sessenta dias antes do parto), T-30 (trinta dias antes do parto), T-15 (quinze dias antes do parto), T0 (primeiras seis horas após o parto), T+15 (quinze dias após o parto), T+30 (trinta dias após o parto) e T+60 (sessenta dias após o parto). As éguas do GM foram avaliadas apenas no T-60. Todos os animais foram submetidos a avaliações clínicas: peso corporal, temperatura retal, coloração da mucosa, tempo de enchimento capilar, porcentagem de gordura e massa livre de gordura, exceto durante o parto; análises hematológicas: eritrograma, leucograma (total e diferencial) e contagem de plaquetas; e as análises bioquímicas: proteína sérica, albumina, globulina, ferro, fibrinogênio, colesterol total, triglicerídeos, ácidos graxos não-esterificados, glicose, lactato, cálcio total, fósforo, magnésio, sódio, potássio, cloreto, ácido úrico, creatinina, ureia, gamaglutamiltransferase, aspartatoamiltransferase, fosfatase alcalina e creatina quinase. Os resultados foram submetidos a tratamento estatístico que demonstrou maior temperatura retal nos tempos T+15, T+30 e T+60 do que nos T-60, T-30 e T-15. O peso corporal e a massa livre de gordura diferiu (P < 0,05) entre as éguas em manutenção e as do período de transição. No GT houve uma redução progressiva de ambas as variáveis (P < 0,05). A porcentagem de gordura foi menor nas éguas em manutenção. Houve diferença entre os grupos (P < 0,05) na quantidade de colesterol, triglicerídeos, ácidos graxos não-esterificados, glicose e lactato. O GT apresentou menor (P < 0,05) concentração de hemácias, hematócritos e hemoglobina do que no GM. O GM apresentou menores valores de volume corpuscular médio e de distribuição do diâmetro das hemácias com coeficiente de variação e maiores valores da concentração de hemoglobina corpuscular média. Não ocorreu diferença (P > 0,05) nos valores de hemoglobina corpuscular média, leucócitos global, neutrófilos segmentados e bastonetes, e ferro. As plaquetas diferiram apenas no GT (T-60) e as globulinas no GT (T0 e T+15). Houve diferença entre grupos (P < 0,05) nos valores de monócitos, eosinófilos, linfócitos, proteínas totais, fibrinogênio e albumina. Houve diferença (P < 0,05) entre os grupos GM e GT e entre os tempos avaliados nas quantidades de cálcio, magnésio, sódio, potássio, creatinina, ureia, fosfatase alcalina e creatina quinase. Já o cloreto e o fósforo não diferiram (P > 0,05) entre as éguas em manutenção e as em transição, assim como as concentrações enzimáticas de gamaglutamiltransferase e aspartatoaminotransferase. O ácido úrico também diferiu entre os grupos (P > 0,05), e GT apresentou diferença ao longo dos tempos. O período de transição exerce forte influência na condição corporal e nos elementos presentes no sangue das éguas evidenciando a demanda metabólica da égua e do feto. Muitas das adaptações são fisiológicas e decorrem de mecanismos de proteção do organismo materno para garantir que esta possa suprir as necessidades impostas pelo período. É possível monitorar a saúde da égua bem como a do feto a partir do conhecimento e avaliação do perfil metabólico de éguas submetidas ao mesmo manejo. The transition period in mares comprises the end of gestation, delivery and initiation of lactation. At this stage the adaptations in the mare organism are intense and are associated with an altered metabolic state when compared to the non-gestational period. The present study aimed to evaluate the effects of the transition period on clinical and laboratory parameters in Mangalarga Marchador mares. A total of 48 mares were used, distributed into two groups: the maintenance group (MG), formed by non-pregnant mares, and an experimental group, called the transition group (TG), composed of pregnant mares and after delivery infants. T-60 (sixty days before delivery), T-30 (thirty days before delivery), T-15 (fifteen days before delivery), T0 (first six hours after delivery), T+15 (fifteen days after delivery), T+30 (thirty days after delivery) and T+60 (sixty days after delivery). GM mares were evaluated only on the T-60. All animals were submitted to clinical evaluations: body weight, rectal temperature, mucosal staining, capillary filling time, percentage of fat and fat free mass, except during delivery; hematological analyzes: erythrogram, leukogram (total and differential) and platelet count; and biochemical analyzes: serum protein, albumin, globulin, iron, fibrinogen, total cholesterol, triglycerides, non-esterified fatty acids, glucose, lactate, total calcium, phosphorus, magnesium, sodium, potassium, chloride, uric acid, creatinine, urea, gammaglutamyltransferase, aspartate amyltransferase, alkaline phosphatase and creatine kinase. The results were submitted to statistical treatment that showed a higher rectal temperature at T+15, T+30 and T+60 times than in the T-60, T-30 and T-15 times. Body weight and fat free mass differed (P < 0.05) between the maintenance mares and those of the transition period. In TG there was a progressive reduction of both variables (P < 0.05). The percentage of fat was lower in maintenance mares. There was a difference between groups (P < 0.05) in the amount of cholesterol, triglycerides, non-esterified fatty acids, glucose and lactate. The TG presented lower (P < 0.05) concentration of red blood cells, hematocrits and hemoglobin than in MG. The MG presented lower values of mean corpuscular volume and distribution of red blood cell diameter with coefficient of variation and higher values of mean corpuscular hemoglobin concentration. There was no difference (P > 0.05) in mean corpuscular hemoglobin, global leukocytes, segmented neutrophils and rods, and iron. Platelets differed only in TG (T-60) and TG globulins (T0 and T+15). There were differences between groups (P < 0.05) in the values of monocytes, eosinophils, lymphocytes, total proteins, fibrinogen and albumin. There was a difference (P < 0.05) between the MG and TG groups and between the times evaluated in the amounts of calcium, magnesium, sodium, potassium, creatinine, urea, alkaline phosphatase and creatine kinase. Chloride and phosphorus did not differ (P > 0.05) between maintenance and transitional mares, as did the enzymatic concentrations of gammaglutamyltransferase and aspartate aminotransferase. Uric acid also differed between groups (P > 0.05), and TG presented a difference over time. The transition period exerts a strong influence on the body condition and the elements present in the blood of the mares evidencing the metabolic demand of the mare and the fetus. Many of the adaptations are physiological and stem from mechanisms of protection of the maternal organism to ensure that it can meet the needs imposed by the period. It is possible to monitor the health of the mare as well as the health of the fetus from the knowledge and evaluation of the metabolic profile of mares submitted to the same management.

O objetivo desse trabalho foi produzir e testar uma tecnologia que permite a fabricação e aplicação de marcador visual externo para peixes, economicamente viável e que apresente boa taxa de retenção sem comprometer o desempenho dos animais. Primeiramente, foi desenvolvido um marcador externo (deposito BR1020170075770) que consiste em marcadores fabricados via corte a laser de chapas do polímero PETG e aplicador que compreende uma agulha, uma trava e um suporte. A tecnologia desenvolvida foi testada em Pacamã (Lophiosilurus alexandri) sendo realizados dois experimentos. No primeiro foi verificado a taxa de retenção e a influência do marcador sobre a sobrevivência e ganho de peso de 3 classes de tamanho de L. alexandri: Pequeno (10,58±0,63 cm e 14,50±0,63 g), Médio (11,71±0,52 cm e 23,95±1,76 g) e Grande (13,5±0,60 cm e 31,96±3,50 g) durante 90 dias. Foram utilizadas 4 repetições para cada tamanho de animal, cada repetição era composta de 25 animais, sendo 15 marcados e 10 não marcados. A taxa de retenção dos marcadores foi calculada a cada 30 dias e o ganho de peso dos animais no final dos 90 dias. No segundo experimento foi verificada a influência do marcador sobre os parâmetros hematológicos dos juvenis (17,09 ± 1,02 cm e 64,28±11,80 g) após 5, 10 e 30 dias da marcação frente a animais não marcados. No experimento 1, os animais apresentaram 100 % de sobrevivência. O marcador apresentou maior taxa de retenção (P

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El documento consiste en la determinación de valores referenciales en parámetros sanguíneos de caninos machos a 2550msnm, con el fin de establecer datos propios al medio, que servirá de ayuda a los especialistas para que se logre implementar un diagnóstico auténtico del estado clínico del paciente tratante. This document consists in the determination of reference values of blood parameters in male canines in Cuenca, in order to establish their own data in the medium, which will help the specialists in the subject, manage to achieve an authentic diagnosis of the clinical condition of the treating patient.

El documento consiste en la determinación de valores referenciales en química sanguínea y hemograma en caninos hembras localizadas en Cuenca, con el fin de establecer datos propios al medio, que servirá de ayuda para que los especialistas en el tema logren implantar un diagnóstico auténtico del estado clínico del paciente tratante. This document consists in the determination of referential values of blood chemistry and hemogram in canine females located in Cuenca, in order to establish their own data in the medium, which will help the specialists in the subject manage to achieve an authentic diagnosis of the clinical condition of the treating patient

FAPEMIG - Fundação de Amparo a Pesquisa do Estado de Minas Gerais Os parâmetros hematológicos são bons indicadores para avaliar a saúde do animal e úteis na identificação de possíveis desordens. Vacas no período pós-parto passam por estresse fisiológico que pode causar alterações significativas nesses parâmetros, refletindo na saúde e produtividade do animal. Objetivou-se o presente estudo investigar alterações nos parâmetros hematológicos em vacas mestiças lactantes na primeira semana e aos 60 dias pós-parto (PP). Nas 110 vacas mestiças recém-paridas estudadas foram realizadas duas coletas de 5mL de sangue: a primeira coleta na primeira semana e a segunda aos 60 dias PP. Os hemogramas foram processados em analisador hematológico automático veterinário pocH - 100iV. A contagem diferencial dos leucócitos foi realizada em esfregaço sanguíneo corado pelo método de May Grunwald Giemsa. Em cada esfregaço sanguíneo foram diferenciados 100 leucócitos classificados, de acordo com suas características morfológicas e tintoriais. A determinação dos teores plasmáticos de proteínas (PTP) foi realizada pelo método do biureto após centrifugação das amostras de sangue. Comparados os valores dos parâmetros hematológicos dos animais entre os dois períodos estudados, observaram-se diferenças significativas (p

Fundação de Amparo à Pesquisa do Estado de Minas Gerais A presença de animais silvestres em área urbana está se tornando cada vez mais comum e devido à alterações no habitat, muitas espécies vivem em pequenas populações com variados graus de isolamento. A interferência antrópica no ambiente deixa os animais mais vulneráveis e facilita o surgimento de doenças. As análises dos constituintes do sangue e os valores dos parâmetros bioquímicos são ferramentas importantes no diagnóstico de doenças, permitindo avaliar o estado de saúde do animal, mesmo antes do aparecimento de sintomas. Considerando a escassez de dados para a espécie em questão e a necessidade de estabelecer valores que possam contribuir com estudos futuros, o presente estudo tem como objetivo apresentar o valores hematológicos e bioquímicos de saguis de vida livre da espécie Callithrix geoffroyi em diferentes áreas urbanas e peri-urbanas no estado do Espírito Santo. O estudo foi conduzido nos municípios de Vitória, Cariacica e Vila Velha, pertencentes à região metropolitana da grande Vitória. Foram coletados sangue de 30 indivíduos da espécie C. Geoffroyi, em seis diferentes localidades. Adultos foram significativamente mais pesados quando comparados aos jovens. Quanto a análise celular sanguínea, não foram observadas diferenças entre machos e fêmeas, sendo que jovens e adultos diferiram para eritrócitos, hemoglobina e plaquetas, reflexo de uma maior massa muscular de adultos e uma maior sensibilidade de jovens a homeostase hídrica. Quanto aos parâmetros bioquímicos, machos e fêmeas diferiram quanto ao colesterol, AST e globulinas. Estes parâmetros estão dentro da faixa descrita para a espécie e variações podem advir de diferenças na dieta, na função hepática e de infecções. Jovens e adultos diferiram quanto aos valores de proteínas totais, sendo pouco claro os prováveis motivos para este achado. Em relação aos locais de coleta, diferenças significativas foram descritas tanto para parâmetros morfométricos (IMC) quanto para os parâmetros bioquímicos (ALT, uréia, creatinina e glicose) e celulares sanguíneos (CHCM, segmentados, linfócitos e monócitos), sendo que estes parecem estar associados às variações nas condições ambientais, fisiológicas e nutricionais. Apesar deste estudo ter identificado diferenças significativas para alguns dos parâmetros analisados, todos os animais apresentaram-se saudáveis e os valores se assemelham aos apresentados para outras espécies do gênero Callithrix. Um maior número de estudos sobre as mudanças na estrutura e ecologia de comunidades, sobre o comportamento e sobre os fatores que permitem maior resiliência de espécies em áreas urbanas e peri-urbanas, além da análise nutricional dos alimentos comumente consumidos poderão auxiliar na compreensão sobre como a aproximação entre humanos e saguis tem interferido nos hábitos e na saúde desta espécie. The presence of wild animals in urban areas is becoming increasingly common and due to changes in habitat, many species live in small populations with varying degrees of isolation. Anthropogenic interference in the environment leaves animals more vulnerable and facilitates the onset of disease. Analyzes of blood constituents and biochemical parameters are important tools in the diagnosis of diseases, allowing the evaluation of the health status of the animal, even before the appearance of symptoms. Considering the scarcity of data for the species in question and the need to establish values that may contribute to future studies, the present study aims to present the hematological and biochemical values of free- living marmosets of Callithrix geoffroyi in different áreas urban and peri-urban in the state of Espírito Santo. The study was conducted in the municipalities of Vitória, Cariacica and Vila Velha, belonging to the greater Vitória metropolitan region. Blood samples were collected from 30 C. geoffroyi species in six different locations. Adults were significantly heavier when compared to young marmosets. Regarding blood cellular analysis, no differences were observed between males and females, and young and adult differed for erythrocytes, hemoglobin and platelets, reflecting a greater muscular mass of adults and a greater sensitivity of young people to water homeostasis. Regarding the biochemical parameters, males and females differed for cholesterol, AST and globulins. These parameters are within the range described for the species and variations may arise from differences in diet, liver function and infections. Young and adults marmosets differed in total protein values, and the probable reasons for this finding were unclear. Regarding the collection sites, significant differences were described for both morphometric parameters (IMC) and biochemical parameters (ALT, urea, creatinine and glucose) and blood cells (CHCM, segmented, lymphocytes and monocytes). Associated with variations in environmental, physiological and nutritional conditions. Although this study identified significant differences for some of the analyzed parameters, all the animals were healthy and the values are similar to those presented for other species of Callithrix genus. A larger number of studies on changes in community structure and ecology, behavior and factors that allow species resilience in urban and peri-urban areas, and nutritional analysis of commonly consumed foods may help in understanding how the approach between humans and marmosets has interfered in the habits and health of this species.

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2017. As raças Pantaneiro e Campeiro foram criadas, por muito tempo, somente dentro de seus Estados de origem. Como consequência, um baixo número de exemplares foi gerado, e como forma de conservação de recursos genéticos, torna-se necessário estabelecer os padrões reprodutivos e clínicos desses animais como forma de implantar novas biotecnologias aplicadas à reprodução. Objetivou-se realizar uma avaliação descritiva da determinação da idade fetal por meio da medição da órbita ocular dos fetos de éguas das raças Campeiro e Pantaneiro e também avaliar as mudanças sequenciais na hematologia e bioquímica de potros dessas raças, do nascimento aos seis meses de vida, através da mensuração do número de hemácias, concentração de hemoglobina (Hb), volume corpuscular médio (VCM), concentração de hemoglobina corpuscular média (CHCM), leucócitos (Leuc), linfócitos (Linf), eosinófilos, monócitos, basófilos, número de plaquetas (Plt), fibrinogênio (Fib), albumina (Alb), proteínas plasmáticas totais uréia, creatinina (creat), aspartato aminotransferase (AST), fosfatase alcalina (FA), gama glutamil transferase (GGT) e globulinas (Glob). Foram utilizadas 10 éguas, sendo 5 da raça Pantaneiro e 5 da raça Campeiro. O modelo de regressão que melhor se aplicou para relacionar essas duas variáveis é o modelo de regressão linear. O modelo obtido para a raça Campeiro foi y = -55,802+11,1x (R2 = 0,977 e P

Este artículo se encuentra disponible en la siguiente URL: https://cjas.agriculturejournals.cz/pdfs/cjs/2017/06/05.pdf Many physiological events occurring during the estrous cycle, including folliculogenesis, oocyte maturation, ovulation, follicular atresia, corpus luteum development, luteolysis, steroidogenesis, and angiogenesis are associated with an increased expression of the renin–angiotensin–aldosterone system (RAAS) in uterus and ovarian follicles. This enhanced expression leads to a rise in plasma concentrations of these hormones in women and laboratory animals, and the same might happen in the mare. This study aims to assess if an activation of the RAAS occurs in mares around ovulation and if this activation is related to the diameter of the predominant follicle (DPF), packed cell volume (PCV), and electrolyte concentrations. Twenty-five healthy Spanish Purebred mares were sampled during the five days before ovulation, the day of ovulation, and the first five days after ovulation. Renin (REN) concentrations increased progressively during the five days before ovulation, achieving the highest values on the day of ovulation. Angiotensin (ANG) concentrations showed a sharp decrease after ovulation, with the lowest values the fifth day after it. Aldosterone (ALD) concentrations increased progressively, from the fifth day before ovulation until the fifth day after ovulation. The highest PCV values were found on the day of ovulation. There were no relevant correlations between the RAAS components and electrolytes concentrations. Before ovulation, there was a positive relationship between REN and ALD (r = 0.760) and after ovulation, a negative correlation between ANG and ALD (r = –0.660). The DPF was correlated with REN (r = 0.740) and with ALD (r = 0.800) concentrations. Ovulation in the mare is associated with high plasma REN concentrations, and therefore, with an activation of the RAAS. In addition, after ovulation, the sharp decrease in plasma REN and ANG concentrations might be a reflex of the modulation of the previously activated RAAS, although plasma ALD concentrations increased during this period.

BACKGROUND The Black vulture (Coragyps atratus) is the most common species of vulture and is widespread in all America. The species feeds on rotting carcasses, and large groups are frequently seen in urban areas, concentrating especially on rubbish dumps. Although C atratus is a very common species in some areas, little is known about its health in the wild. OBJECTIVES The aim of this study was to determine hematologic RIs of wild adult Black vultures. METHODS Blood samples were obtained from 70 wild Black vultures captured in Sao Paulo, Brazil. Hematologic values were determined using conventional techniques applicable to birds. Reference intervals were determined using an Excel program with Reference Value Adviser (version 2.0). RESULTS After statistical analysis, the following RIs were determined: HGB 8.5-12.5 g/dL, PCV 42.3-54.5%, MCV 203.2-402.6 fL, MCHC 17.4-26.2 g/dL, total solids 2.4-4.8 g/dL, RBC count 1.16-2.48 × 106 /μL, WBC count 5.93-27.14 × 103 /μL, heterophils 3.40-21.58 × 103 /μL, lymphocytes 0.19-5.16 × 103 /μL, eosinophils 0.0-3.07 × 103 /μL, monocytes 0.0-1.49 × 103 /μL, basophils 0.0-0.25 × 103 /μL, and heterophil:lymphocyte ratio 1.3-36.9. Thrombocyte mean was 14.14 × 103 /μL. CONCLUSIONS Baseline hematologic data obtained in this study provide RIs that will be useful given that few studies have been carried out on the health of New World vultures.

Objetivou-se com essa revisão de literatura discutir variações individuais, ambientais e de treinamento nos parâmetros hematológicos equídeos. Espécie, raça, idade, sexo e estádio fisiológico, fotoperíodo e época do ano, nutrição e estacionalidade na produção forrageira, exercício e treinamento podem in-terferir em variáveis sanguíneas. O perfil hematológico e bioquímico sanguíneo pode auxiliar a avaliar zootecnicamente equídeos, inclusive na seleção de animais mais tolerantes ao esforço. A ausência de valores de referência para as condições brasileiras pode dificultar a interpretação das análises. O condicionamento físico possui reflexos diretos no desempenho esportivo, nas células e outros compo-nentes do sangue de equinos. Portanto, análises sanguíneas devem ser realizadas em animais atletas, para auxiliar no monitoramento do treinamento.Palavras-chave: Equus asinus. Equus caballus. Hemograma. Bioquímica sérica. This literature review aimed to discuss individual variations, environmental influences and training inter-ference in the equidae hematological parameters. Species, breed, age, sex and physiological phases, photoperiod and year season, nutrition and seasonality in forage production, exercise and training may influence hematological parameters. The hematological and biochemical profile may help to evaluate equidae zootechnically, including the selection of animals more tolerant to the effort. The absence of ref-erence values for brazilian conditions can hinder the interpretation of results. The physical conditioning of equine species has a direct effect on sports performance, cells and other blood components. There-fore hematological analysis should be performed in athlete animals.Therefore hematological analysis should be performed in athlete animals, to help the monitoring of training. Keywords: Equus asinus. Equus caballus. Haemogram. Serum biochemistry.

This work is part of a research project that aims to produce a natural red food colorant, stable during long-term cold storage, with a wide range of applications, using hemoglobin from porcine blood as raw material. The objective of this study was specifically to assess the suitability of the spray-drying technology to microencapsulate the hem-derivative into a protective matrix, aiming at obtaining a red powder able to maintain its red color in a wide range of pH. To achieve the objective, three separate formulations of haemoglobin derivatization, were prepared by addition of 3.5% nicotinamide as well as, 10% sucrose and the convenient quantity of encapsulation polysaccharide (E1 or E2) in A and C experiments, and 6% of sucrose, 0,1% of E1 and 4 % of a chelating agent in experiment B. Every hem-derivative was then encapsulated and dried in a spray-dryer. After dehydration process, several quality parameters of each encapsulation product, such are the proximate composition, the color, and the water activity, were used to assess the suitability of the encapsulation method and the matrix that leaded to the better quality characteristics. Moreover, gelatine gels were used as a model system to test the coloring power of both encapsulated products at different pH conditions. The results showed that all the spray-drying encapsulation of hem-derivatives permitted obtaining powders showing water content and water activity (aw) low enough to warrant the microbiological stability without favoring iron oxidation reactions. Immediately after dehydration, all the products exhibited an acceptable bright red color, whatever the encapsulation agent used in the derivatization of hemoglobin. The colorant containing polysaccharide E1 showed better color stability than the two products containing polysaccharide E2. The red color of the E1 powder remained constant over 2-month chill storage. The partial substitution of sucrose by a chelating agent in the products containing the polysaccharide E2 negatively affected the color stability of the dehydrated products. At pH 6-7 all the gelatins containing the hem-colorants showed a bright red color. Nevertheless, only the formulation with combination of chelating agent and polysaccharide E2 was able to preserve the red color at pH below 5. At pH 4.5 the formulation with the same combination did not succeed to complete stabilize redness during storage, since a 30% decrease in a* values occurred after 7-day chill storage. However, final a* values of these gelatins were even 3-fold higher than the initial ones in gelatins containing E1 or E2 without the chelating agent. At pH above 5, although there were significant differences in b* and L* parameters, the a* coordinates showed to be similar for all the treatments at the end of 7-day chill storage, the mean reduction from the initial values being in the range from 6 to 11%

O trabalho foi desenvolvido com o objetivo de montar um perfil hematológico e bioquímico, estabelecendo possíveis diferenças entre sexo e idade, de macacos-prego (Cebus spp.) mantidos em cativeiros, clinicamente saudáveis. Foram determinados valores hematológicos e mensurados valores bioquímicos para determinação do perfil hepático (ALT, AST, FA, GGT), perfil renal (CREAT e ureia), perfil lipídico (COL e TRIG), perfil proteico (ALB e PT), minerais (CA e P), CK-Total e frutosamina. Foram coletadas amostras de sangue de 20 animais (10 machos e 10 fêmeas) mantidos no Zoológico da Universidade de Passo Fundo. A análise estatística empregada foi a descritiva para obtenção da média, do desvio padrão, e de valores mínimos e máximos com 95% de confiança. Não foi encontrada diferenças significativas nos valores hematológicos e bioquímicos, para ambos os sexos e faixas etárias estudadas, de macacos-prego. Desse modo, tais valores encontrados corroboram com a literatura existente e aumentam o número de informações disponíveis aos clínicos de animais selvagens. The study was conducted with the aim to mount a hematological and biochemical profile, establishing possible differences between sex and age, capuchin monkeys (Cebus spp) kept in captivity, clinically healthy. Hematological values were determined as well as biochemical tests to avaliate the liver profile (ALT, AST, ALP and GGT), renal profile (CREAT and urea), lipids profile (COL and TRIG), protein profile (ALB and TP), minerals (CA and P), apart from CK-Total and fructosamine. Blood samples were collected from 20 animals (10 males and 10 females) kept in the Zoo at the University of Passo Fundo. It was employed one statistical descriptive, obtaining the mean, standard deviation, and minimum and maximum values with 95% confidence. There was no significant difference in hematological and biochemical values for both sexes and age groups of capuchin monkeys. Therefore, these results corroborate with the present literature, increasing the quantity of information available to Wildlife clinical.

The high demand of food, adequate nutrition levels, as well as the decreasing on the availability of raw materials, are topics that nowadays require our attention. However, the growing concern about the increasing on pollution and the thousands of tons of waste that end up in our environment have become important matters. What can we do and how can we solve the problem of waste? Specifically, the meat industry, which is one of the industries that has a lower yield (despite its size and the amount of gross sales carried out), is the one that requires more effort in terms of reducing expenses; by renewing its methods and plant processes, waste recovery and the reuse of the leftovers, they can transform the waste into useful products for human and animal consumption. The blood that remains after slaughter of livestock is one of the products that is currently under investigation for its recovery and exploitation, thanks to its nutritional characteristics, its high content in proteins and specially because of the heme iron that it contains. The aims of this work were the characterization and the improvement of the stability of a food colorant, which had been previously developed by the Food Technology research group of INTEA. The colorant consisted on a haemoglobin-based powder derived from hygienically collected porcine blood suitable for human consumption purposes. The drying system used in this work, called spray drying, provides a powdered product that maintains an acceptable red colour during all the process but tends to worsen during storage. The encapsulation of the haemoglobin derivative, which can be achieved simultaneously to the dehydration during the spray-drying process, should allow an improvement in stability and expand the pH range at which it can be applied. Taking into consideration the results of this work, despite the variability due to the raw materials and the drying process, the obtained product was sufficiently homogeneous and showed a good colour. The product has low moisture content and water activity. The amount of ash, as well as the high percentage of proteins, suggests that the product shows a high nutritional value and can be used as a supplement in diets that are poor in nutrients

Pòster Juny

Pòster Juny

L’objectiu general d’aquest treball va ser establir una fórmula que permetés elaborar pernil cuit sense polifosfats afegits, utilitzant plasma concentrat procedent de sang de porc com a ingredient de la salmorra (en substitució dels polifosfats i l’aigua) i, a la vegada, obtenir un producte sense tants additius i més econòmic. En primer lloc es va separar el plasma sanguini de la fracció cel•lular mitjançant centrifugació de la sang recollida en un escorxador industrial. Les mostres de sang contenien tripolifosfat sòdic al 4% (p/v) com a anticoagulant. A posteriori, el plasma es va concentrar fins al 50% mitjançant un procés d’ultrafiltració tangencial en un equip de planta pilot de filtració per membrana. L’objectiu d’aquesta etapa va ser el de concentrar les proteïnes del sèrum. Per dur-ho a terme es va utilitzar una membrana espiral de poliacrilonitril modificat de 30 kDa de pes molecular de tall i una superfície de filtració de 0,32 m2. D’aquesta etapa se’n va fer un seguiment tant del flux de permet recuperat com de la temperatura del retentat

CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Outra Agência Anthelminthic resistant populations of Haemonchus contortus are a major problem in sheep rearing, but plant extracts may offer viable alternative treatments. In our preliminary studies, Piptadenia viridiflora was frequently selected by sheep grazing in the Cerrado. The present research evaluated its in vitro and in vivo anthelmintic activity. The HPLC chromatograms of P. viridiflora aqueous extract (AE) and ethanolic extract (EE) showed the presence of flavonoids. The total condensed tannin (proanthocyanidin) was 0.2 and 1.01% in AE and EE, respectively. In an egg hatching inhibition (EHI) test, the LC90 of AE was 2.4 mg/mL, and, of EE, was 2.1 mg/mL. After tannin extraction, higher EHI and lower LC90 were observed. In a larval development inhibition test, the LC90 of AE was 13.66 mg/g of fecal culture. The highest dose of AE administered to mice (203.0 mg/kg bw) was well tolerated, suggesting low toxicity. In vivo, AE was orally administered to lambs at 283 mg/kg bw, and, at weeks one, two, and three post-treatment, the mean fecal egg count (FEC) was significantly lower than in untreated lambs (P < 0.05). Blood parameters were normal and similar in untreated and treated sheep. For all lamb groups, the mean total serum protein was significantly higher at week two post-treatment than at other evaluated periods (P < 0.05). Piptadenia viridiflora extracts had low condensed tannin content and exhibited high anthelminthic efficacy in vitro and significantly reduced FEC. Tannins were not shown to be the principal components affecting EHI, hence it is necessary to isolate and characterize the principal active P. viridiflora compounds, and to assess their possible synergism.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Este trabalho teve como objetivo determinar o período de recuperação do hematócrito de cães doadores após múltiplas doações de sangue, além de avaliar a resposta reticulocitária de animais suplementados e não suplementados com ferro e vitaminas de influência hematopoiética. Para isto, foram utilizados 10 cães adultos, de ambos os sexos, com peso variando entre 25 e 30 kg, de comportamento dócil, com hematócrito igual ou superior a 40% e em boas condições de saúde. Os animais foram separados em dois grupos, contendo cinco cães cada, sendo um grupo (G1) formado por cães que não receberam suplementação de vitaminas e minerais, além das contidas na alimentação e o outro grupo (G2) formado por cães que receberam suplementação à base de ferro e vitaminas do complexo B. Foram realizadas três coletas de 16 ml/kg de sangue de cada animal em intervalos de 15 dias entre as coletas. Foi mensurado o hematócrito antes de cada doação (T0) e imediatamente após a doação (T1) seguidas de mensurações diárias, em conjunto com a contagem de reticulócito, até que os animais recuperassem o hematócrito inicial. A análise dos resultados mostrou redução significativa de até 2% do hematócrito de T0 para T1, no entanto a redução máxima só pode ser observada pós 24 horas. O grupo dos animais não suplementados apresentou o início de recuperação do hematócrito no quarto dia, na primeira e na segunda doação, porém na terceira doação essa recuperação iniciou no quinto dia. Já o grupo dos animais suplementados apresentou o início de recuperação no quarto dia após primeira coleta, porém na segunda e terceira doação a recuperação iniciou no terceiro dia. Os cães não suplementados recuperaram o hematócrito após 10 dias da doação, enquanto os cães suplementados em apenas 7 dias. A análise dos reticulócitos mostrou que o grupo que não recebeu suplementação atingiu pico de produção em 5 dias enquanto o grupo que recebeu suplementação atingiu o pico em 3 dias. Os valores máximos de reticulócitos não foram afetados pelo tratamento. Entretanto, houve diminuição desses valores entre a segunda e a terceira doação e entre a primeira e a terceira para ambos os grupos. Conclui-se que os cães do grupo suplementado apresentaram uma recuperação do hematócrito mais rápida após a doação, além disto, os cães foram capazes de doar 3 vezes em intervalos de 15 dias sem danos aparentes a saúde, embora aparentemente houvesse indício desgaste medular. This present work aimed at determining the recovery period of the hematocrit level in blood donor dogs after multiple blood donations. It also intended to evaluate the reticulocyte response in animals receiving and animals not receing supplementation with iron and vitamins of hematopoietic influence. For this purpose, 10 adult dogs of both sexes were studied. Their weights varied between 25 and 30 kg, all of them were well behaved and docile, with the hematocrit levels at 40% or higher, and all in perfect good health. The animals were divided into two groups, with five dogs in each. The first group (G1) consisted of dogs that did not receive any supplementation of vitamins and minerals in addition to the ones already contained in their diet. The second group (G2) was formed by dogs that received iron and B complex vitamin supplementation. There were three blood collections of 16 ml/kg each, in each animal, every 15 days. The hematocrit was measured immediately before each donation (T0) and immediatly after as well (T1). In the following days the hematocrit and reticulocyte were measured to the point when the animals recovered their initial levels of hematocrit. The analysis of the results revealed a significant hematocrit decline of up to 2% from T0 to T1. However, the maximum reduction could only be noticed after 24 hours. The group of animals without supplementation exhibited the begining of hematocrit recovery in the fourth day in the first and second donations. However, in the third donation the recovery only started in the fifth day. In the group of animals with supplementation the begining of the recovery was observed in the fourth day after the first donation, but in the second and third donations the recovery began in the third day. The dogs that did not receive the supplementation recovered the hematocrit levels 10 days after the donations, while the dogs that received the supplementation recovered the initial volume in just 7 days. The analysis of the reticulocyte showed that the group that did not receive the supplementation reached a reticulocyte production peak in approximately 5 days, whereas the group that received the supplementation reached the production peak in approximately 3 days. The maximum values of reticulocyte were not affected by the treatment. However, there was a decrease of those values between the second and the third donation and between the first and the third donation in both groups. Thus, it can be concluded that the dogs from the group that received the supplementation showed time optimization in their blood recovery after each donation. Furthermore, the dogs were able to donate blood 3 times in an 15-day interval with no apparent damage the their health.