Search

Additional file 1: Figure S1. Nested and tandem PRE1. a Mechanisms of the formation of nested PRE1 and tandem PRE1. b A PRE1 inserted into another PRE1 tail. The target motif is located in the PRE1 tail (chr7:29710723:29711249). The nested PRE1 are actually two new tandem PRE1 with changed tile lengths. The tail of the basal PRE1 at the 5′ end is shortened, but the tail of the embedded PRE1 at the 3′ end is extended. Target site duplications (TSD) are shown in red font. Embedded PRE1 are shown in blue font, and basal PRE1 are shown in green font. c Repeated TSD produce three tandem PRE1 (chr12:33294823-33295640); d A PRE1 inserted into another PRE1 body. The target motif is located in the PRE1 body, which leads to a nested PRE1 (chr14:22207023-22207668); and e A tandem PRE1 inserted into the genome (chr2:80470307-80470742). Figure S2. Internal repeat segments in PRE1. a The consensus sequence of quasi-oligomeric PRE1 including three direct repeats. The black arrows indicate the three direct repeats; b Several rare PRE1 with other direct repeats in the PRE1 body. Four repeat segments of PRE1 (chr15:28050427-28050730, chr6:166491294-166491728, chr7:65896533-65896843; and chr15:77600095-77600373) have been marked in the consensus sequence. The arrows show the duplicated segments in different PRE1; and c The degenerate 46-bp repeat sequence in PRE1. The blue dotted arrows show the degenerate 46-bp repeat sequence, and the green arrows show the direct repeats. Figure S3. Three alternative alignment methods for PRE1d2. The PRE1d2 shows a degenerate 46-bp segment following the splicing model, and PRE1d2(2) and PRE1d2(3) show hypothetically missed whole R or F direct repeats, respectively. Figure S4. Differentially-methylated PRE1 in the promoter regions of the HACD3 and HORMAD1 genes.

Ag-SnO 2 coating on copper substrate has been prepared by an atmospheric plasma spraying method using high energy ball milled Ag-12wt%SnO 2 composite powder as raw material. The microstructures of the Ag-SnO 2 coating were characterized by XRD and SEM. The mechanical properties and arc erosion performance of the as sprayed coating have been determined by tensile test, microhardness and arc erosion tests, respectively. The results show that the Ag-SnO 2 coating presents a compact microstructure, and the nanosized SnO 2 particles are uniformly dispersed in the Ag matrix of the coating. The mechanical properties and the arc erosion performance of the Ag-SnO 2 coating are close to those of Ag-SnO 2 bulk. After arcing test, the surface of the coating presents the dispersion of cathode spots and a little erosion, indicating that the Ag-SnO 2 coating has excellent arc-erosion resistance. Plasma spray technique is an effective approach to manufacture Ag-SnO 2 contact coating with good mechanical properties and arc erosion resistance.