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1. Nanoscale control of oxides by laser ablation: design and applications

Author

Prellier, Wilfrid, Singh, M., Khokhlova, M, Hammad, M, Lhuissier, E, Retoux, R, Goux, D, Fouchet, A, David, A, Lüders, U, Boumediene, Karim, Laboratoire de cristallographie et sciences des matériaux (CRISMAT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Max-Born Institute for Nonlinear Optics and Short Pulse Spectroscopy (MBI), Université Catholique de Louvain = Catholic University of Louvain (UCL), Biologie du cartilage, biotechnologie et télémédecine (Bioconnect), Normandie Université (NU)-Normandie Université (NU), Laboratoire Universitaire des Sciences Appliquées de Cherbourg (LUSAC), Centre de Microscopie Appliquée à la Biologie [Caen] (CMABio3), Interactions Cellules Organismes Environnement (ICORE), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), NKE instrumentation, and Biotechnologie des tissus conjonctifs et cutanés / Biology of Connective and Cutaneous Tissues (BioConnecT)

Subjects
[PHYS]Physics [physics], Laser ablation, Materials science, Superlattices, Multifunctional materials, Superlattice, Magnetism, Nanophotonics, Pulsed laser deposition, Oxides, Distortion, Reflection, Nanotechnology, [CHIM.MATE]Chemical Sciences/Material chemistry, Ferromagnetics, Polarization, [CHIM]Chemical Sciences, Calcium, Multiferroics, Nanoscopic scale
Abstract

International audience; Abstract While metal oxides synthesized as thin films have attracted much attention due to their electronic applications, we show here that they can also be used as bio-surfaces for the growth of stem cells, a field of research which has not been much explored. To demonstrate this, thin films (150–200 Å) of titanium oxide (TiO 2 ) and aluminum oxide (Al 2 O 3 ) were deposited on glass using the pulsed laser deposition technique and mesenchymal stem cells were grown on these layers. Cell behavior was evaluated with respect to various key parameters, such as the composition, wettability, morphology and thickness of the films. Our results indicate that thin films of TiO 2 and Al 2 O 3 can not only support stem cell adhesion and growth, but can be used to influence the osteogenic and chondrogenic differentiation paths, which opens the route for a variety of novel biocompatible materials.

Published
2006
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3. BMP-2, Hypoxia, and COL1A1/HtrA1 siRNAs Favor Neo-Cartilage Hyaline Matrix Formation in Chondrocytes

Author

David Ollitrault, Vijayalakshmi Shridhar, Magali Demoor, Philippe Galéra, Denis Vivien, Daniel Hartmann, Karim Boumediene, Hervé Benateau, Frédéric Mallein-Gerin, Laurent Poulain, Didier Goux, Mélanie Briand, Florence Legendre, Alfonso Baldi, Hanane Chajra, Carole Drougard, Ollitrault, D, Legendre, F, Drougard, C, Briand, M, Bénateau, H, Goux, D, Chajra, H, Poulain, L, Hartmann, D, Vivien, D, Shridhar, V, Baldi, Alfonso, Mallein Gerin, F, Boumediene, K, Demoor, M, Galera, P., Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université-Sorbonne Université (SU), Microenvironnement cellulaire et pathologie (MILPAT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN), service de Chirurgie Maxillo-Faciale, Plastique et Reconstructrice, Chirurgie Orale et implantologie [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Interactions Cellules Organismes Environnement (ICORE), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Groupe Régional d'Etudes sur le CANcer (GRECAN), Normandie Université (NU)-Normandie Université (NU)-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-IFR146, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Sérine protéases et physiopathologie de l'unité neurovasculaire, Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biochemistry, Sect Pathology, University of Naples Federico II, Service d'Etudes de Simulation du Comportement du combustibles (SESC), Département d'Etudes des Combustibles (DEC), CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Leibniz Institute for Tropospheric Research (TROPOS), Matériaux, ingénierie et science [Villeurbanne] (MATEIS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie de la Matière Condensée de Paris (site ENSCP) (LCMCP (site ENSCP)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Mateis, Laboratoire, UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU), and UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-IFR146

Subjects
Cartilage, Articular, Bone Morphogenetic Protein 2, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SPI.MAT] Engineering Sciences [physics]/Materials, Mice, 0302 clinical medicine, serine proteinase, 80 and over, cell dedifferentiation, genetics, ComputingMilieux_MISCELLANEOUS, Cells, Cultured, Aged, 80 and over, 0303 health sciences, Cultured, Treatment conditions, adult, bovine, Serine Endopeptidases, High-Temperature Requirement A Serine Peptidase 1, Cell Hypoxia, 3. Good health, Cell biology, Nucleic acids, collagen sponge, priority journal, bone development, 030220 oncology & carcinogenesis, Restoration, Autologous chondrocyte implantations, Collagen, alpha 1 chain, Chondrogenesis, Type I collagen, in vitro study, Cells, Biomedical Engineering, Bioengineering, Bone morphogenetic protein, Small Interfering, Article, animal tissue, 03 medical and health sciences, Chondrocytes, Bone morphogenetic proteins, Public health issues, Humans, articular cartilage, Scaffolds (biology), human, RNA, Messenger, protein expression, mouse, Aged, COL1A1 gene, Cartilage, human cell, Proteins, Hypertrophy, Immunology, Joints (anatomy), HtrA1 protein, Cattle, genetic transfection, Repair, very elderly, Nude, Messenger, Medicine (miscellaneous), [SPI.MAT]Engineering Sciences [physics]/Materials, Degradation, Osteogenesis, cartilage cell, animal, Chondrocyte redifferentiation, RNA, Small Interfering, Autologous chondrocyte implantation, Hyaline cartilage, Chemistry, messenger RNA, Middle Aged, biological marker, unclassified drug, Extracellular Matrix, medicine.anatomical_structure, Body fluids, female, Phenotype, Amino acids, hyaline cartilage, nude mouse, HtrA1 serine protease, Hyalin, animal experiment, Mice, Nude, Bone morphogenetic protein 2, Chondrocyte, Collagen Type I, Innovative strategies, medicine, Articular cartilages, Mus musculus, Animals, controlled study, HtrA1 gene, gene, collagen type 1, 030304 developmental biology, cell culture, nonhuman, in vivo culture, small interfering RNA, Collagen Type I, alpha 1 Chain, osteoarthritis, Kinetics, drug effects, cytology, gene expression, RNA, metabolism, Articular
Abstract

cited By 10; International audience; Osteoarthritis (OA) is an irreversible pathology that causes a decrease in articular cartilage thickness, leading finally to the complete degradation of the affected joint. The low spontaneous repair capacity of cartilage prevents any restoration of the joint surface, making OA a major public health issue. Here, we developed an innovative combination of treatment conditions to improve the human chondrocyte phenotype before autologous chondrocyte implantation. First, we seeded human dedifferentiated chondrocytes into a collagen sponge as a scaffold, cultured them in hypoxia in the presence of a bone morphogenetic protein (BMP), BMP-2, and transfected them with small interfering RNAs targeting two markers overexpressed in OA dedifferentiated chondrocytes, that is, type I collagen and/or HtrA1 serine protease. This strategy significantly decreased mRNA and protein expression of type I collagen and HtrA1, and led to an improvement in the chondrocyte phenotype index of differentiation. The effectiveness of our in vitro culture process was also demonstrated in the nude mouse model in vivo after subcutaneous implantation. We, thus, provide here a new protocol able to favor human hyaline chondrocyte phenotype in primarily dedifferentiated cells, both in vitro and in vivo. Our study also offers an innovative strategy for chondrocyte redifferentiation and opens new opportunities for developing therapeutic targets. Copyright 2015, Mary Ann Liebert, Inc.

Published
2015
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