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1. Cellular reprogramming by Acly inhibition using SB-204990 modulates molecular mechanisms with aging

Author

Sola-García, Alejandro, Cáliz-Molina, María Ángeles, Villanueva, Isabel Espadas, Petr, Michael, Panadero-Morón, Concepción, González-Morán, Daniel, Martín-Vázquez, María Eugenia, Narbona-Pérez, Álvaro Jesús, López-Noriega, Livia, Martínez-Corrales, Guillermo, López-Fernández-Sobrino, Raúl, Marin, Lina M. Carmona, Martínez-Force, Enrique, Yanes, Oscar, Vinaixa, María, López-López, Daniel, Reyes, José Carlos, Dopazo, Joaquín, Martín, Franz, Benoit R. Gauthier, Scheibye-Knudsen, Morten, Capilla-González, Vivian, and Martín-Montalvo, Alejandro

Subjects
Aging, metabolism
Abstract

iTRAQ labeling with two 8-plex iTRAQ kits was performed in the Proteomic facility of the Institute of Biomedicine of Seville using their standard protocols. Individual liver samples were isolated in urea lysis buffer (8 M urea, 25 mM Tris, 100 mM NaCl, 25 mM NaF, 10 mM Na4P2O7, 50 mM β-glycerophospate, 1 mM Na3VO4, 1:100 protease inhibitors and 1:100 deacetylase inhibitors, pH 8). Samples were sonicated for 10 seconds and centrifuged at 20000 x g for 15 min at 4 ºC. The supernatant was harvested and stored at -80º C. Then, samples were reduced with 50 mM tris-(2-carboxyethyl)phosphine (AB Sciex) at 60 °C for 1 hour with shaking and were subsequently alkylated using 200 mM S-methyl methanethiosulfonate (AB Sciex) for 30 min at room temperature. Samples were then trypsinized at 37 °C in a 10:1 ratio (w/w) of substrate/enzyme in a water bath overnight (Promega). Then, samples were speed-vac dried. The iTRAQ-labeling assay was conducted according to the manufacturer’s instructions (iTRAQ 8-plex, AB Sciex). Briefly, peptides were reconstituted in 1M triethylammonium bicarbonate (Sigma-Aldrich St. Louis, MO, USA), 0.05% SDS, 1:100 phosphatase inhibitor cocktail, 1:100 protease inhibitor cocktail, and 0.002% benzonase (Novagen, Argentina) and labeled with one isobaric amine-reactive tag. After 2 hoursof incubation, labeled samples were pooled, dried at 45 °C, and stored overnight at 4 °C. iTRAQ-labeled samples were desalted using Oasis HLB C18 cartridges (Waters, Milford, MA, USA) and dried using a vacuum centrifuge. Peptides were then prefractionated using MCX Oasis columns (Waters) and increasing concentrations (50-2000 mM) of ammonium formate. Fractions were collected, individually washed using C18 cartridges and dried. Peptides from each fraction were separated using nano-liquid chromatography (nano LC 1000, Thermo Scientific) and analyzed by means of nano-electrospray ionization (Proxeon Biosystems, Odense, Denmark) connected to a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific). Briefly, 13 µL of each fraction was loaded, preconcentrated, and washed in an Acclaim PepMap (75 µm × 2 cm, nanoViper, C18, 3 µm, 100 Å) precolumn (Thermo Scientific). Peptides were separated in an analytical column (75 µm × 15 cm, nanoViper, C18, 2 µm, 100 Å, Acclaim PepMap RSLC) for 240 min at 200 nL/min (Thermo Scientific). Peptides were eluted with a gradient of buffer A (0.1% formic acid, 100% H2O) to buffer B (0.1% formic acid, 100% acetonitrile).Files contain data from the different fractions for iTRAQ experiments used for protein detection in RAW format.

Published
2025
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5. Stigmatizing Spaces and Places as Axes of Intersectional Stigma Among Sexual Minority Men in HIV Prevention Research

Author

Tamara Taggart, H. Jonathon Rendina, Cheriko A. Boone, Paul Burns, Joseph Carter, Devin English, Shawnika Hull, Jenné S. Massie, Mary Mbaba, Leandro Mena, Ana María del Río-González, Ore Shalhav, Ali J. Talan, Carly Wolfer, and Lisa Bowleg

Subjects
Male, Acquired Immunodeficiency Syndrome, Sexual and Gender Minorities, Social Stigma, Public Health, Environmental and Occupational Health, Humans
Published
2024

6. Monumental landscapes: spatial analysis of the defensive structures in the Baroña hillfort (Porto do Son, A Coruña)

Author

David González-Álvarez, Rodrigo González-Camino, Axencia Galega de Innovación, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), González-Camino, Rodrigo, and González Álvarez, David

Subjects
landscape archaeology, Archeology, History, society, Edad del Hierro, Iron Age, castros gallegos, phenomenology, fenomenología, Galician hillforts, arqueología del paisaje, sociedad
Abstract

González-Camino R. y González-Álvarez D. (2022). Paisajes monumentales: análisis espacial del dispositivo amurallado del castro de Baroña (Porto do Son, A Coruña). Complutum, 33(2), 455-479. https://doi.org/10.5209/cmpl.84158, [ES] Se plantea que la elección de una determinada ubicación, la constitución material y la organización interna de los poblados fortificados de la Edad del Hierro en el noroeste peninsular se deben a una cualidad aprehensiva del espacio y no únicamente a necesidades productivas o defensivas. Se aplica una metodología basada en análisis geoespaciales para estudiar los dispositivos defensivos de los castros, tomando el enclave de Baroña como caso de estudio. Los resultados nos llevan a apuntar que la morfología y localización del castro, junto a la poliorcética de su sistema defensivo, responden a la búsqueda por parte de sus habitantes de una percepción de seguridad. Esto se vincularía con una tradición constructiva heredada de un pasado conflictivo previo a la Edad del Hierro., [EN] The selection of specific locations for Iron Age hillforts in the North-west of the Iberian Peninsula, along their material constitution and their internal organisation are proposed to be determined by an apprehensive quality of space and not only by productive or defensive needs. We apply a methodology based on geospatial analysis to assess the defensive devices of hillforts, relying on the site of Baroña as our case study. The results lead us to suggest that the morphology and location of the site, together with the poliorcetica of its defensive system, respond to the inhabitants’ search for a perception of security. This would be related to a building tradition inherited from a conflictive past prior to the Iron Age., El trabajo de RGC para preparar este artículo ha sido posible gracias a un contrato de técnico del Programa Investigo de la Xunta de Galicia, financiado por la Unión Europea-NextGenerationEU. El trabajo de DGA ha sido facilitado por un contrato postdoctoral Juan de la Cierva-Incorporación 2019 (Ref. IJC2019-040603-I) financiado por el Ministerio de Ciencia e Innovación del Gobierno de España (MCIN/AEI /10.13039/501100011033).

Published
2022

7. A new species of Chrysobothris (s. str.) Eschscholtz, 1829 (Coleoptera: Buprestidae) from northwestern Peru

Author

Gino Juárez-Noé and Uzbekia González-Coronado

Subjects
General Medicine
Abstract

Chrysobothris (Chrysobothris) fallax sp. nov., collected from dry ecosystems of northwestern Peru is described and illustrated. The species has been confused with Chrysobothris (C.) acutipennis Chevrolat from which it can be distinguished by the form of the tooth on the profemora and the male genitalia. Comments on its ecology and distribution in the country are provided.

Published
2023

9. Family Relations of University Students in Times of Pandemic by COVID-19: Feelings and Tensions

Author

Astrid Sofía Suárez-Barros, María del Pilar Morad-Haydar, Jennifer Castillo- Bolaños, Alfredo Rojas-Otálora, Yomaira García-Acuña, Yolima Alarcón-Vásquez, Yolanda Rosa Morales Castro, Ángela García-Navarro, Orlando González-Gutiérrez, Sarelys Avendaño Escobar, Victoria González-Martelo, Elcie Támara-Arrázola, and Julieth Diaz-Lobo

Subjects
Psychiatry and Mental health
Abstract

Objective: The objective of this research was to reveal the feelings and tensions that characterize the family relationships of university students in times of pandemic and confinement conditions due to COVID-19. Methods: The method was qualitative, and the feelings and tensions in the family and educational context of 584 university students were evaluated, from a qualitative proposal and a hermeneutic approach, anchored to the question: How have the affective manifestations been, in terms of feelings and tensions, in the family relationships of university students, academically active, in the Caribbean Region of Colombia, during the mandatory confinement process derived from the COVID 19 pandemic?. The methodological design was structured from the concepts of the Grounded Theory, applied to the data obtained through virtual techniques and instruments, such as an online survey. The organization of the information to generate the results was made from descriptive, analytical and interpretive coding, supported by matrices and theoretical maps. Results and Discussion: There are feelings and tensions in three directions: Negative Feelings (in tension) such as anxiety, stress and depression; positive feelings, joy, adaptation and ambiguous feelings, which show ambivalence and variability, which are reflected in family relationships and three senses: They have improved, remain the same and have worsened, according to each type, dynamics and characteristics of the family, associated with gender, coping mechanisms and socioeconomic level. Conclusion: Families were transformed due to the pandemic, and relationships and interactions were found that exacerbated tensions or factors of protection and care among family members, with emotional overload, with stress from work and study.

Published
2023

10. Reflexiones acerca del «reasilvestramiento» en la Argentina

Author

María de las Mercedes Guerisoli, Schiaffini, Mauro I., Pablo, Teta, Valenzuela, Alejandro E. J., Patricia, Mirol, Defossé, Guillermo E., María Marcela Godoy, Pablo, Krieger, Tomás, Withington, Agostini, María G., Anderson, Christopher B., Melina, Anello, Gustavo, Aprile, Aquino, Jesica E., Antonella Argoitia, M., Yanina, Arzamendia, Baigorria, Julián E. M., Diego, Baldo, Baldo, Jorge L., Ulises, Balza, Ian, Barbe, Barrasso, Diego A., Barri, Fernando R., Noelia Barrios-Garcia, M., Cristina, Bartolucci, Bastida, Ricardo O., Gabriel, Bauer, Pablo, Berrozpe, Claudio, Bertonatti, Roberto F., Bó, Bracamonte, Julio C., Campo, Denise H., Mariana, Cannizzo, Pablo, Carmanchahi, Flavia, Caruso, Flavia, Cassinelli, Chalukian, Silvia C., Chatellenaz, Mario L., Amelia Chemisquy, M., Mariana, Cosse, Cuyckens, Griet A. E., D’Agostino, Romina L., D’Agostino, Valeria C., Guillermo, Deferrari, Mariana, Degrati, Hebe del Valle Ferreyra, Derlindati, Enrique J., Sophia Di Cataldo, Florencia Di Rocco, Jael, Dominino, Durante, Cristian A., Eder, Elena B., Esquenazi, Sofía M., Ezquiaga, María C., Julián, Faivovich, Fernández, Fernando J., Nicolás, Ferreyra, Francisco Firpo Lacoste, David, Flores, Formoso, Anahí E., Franchini, Marcello, Francisco, Eduardo E., Gómez Fernández, María J., Baltazar, González, González, Enrique M., Susana, González, Guerrero, José C., Guiscafré, Agustín N., Juan, Ernesto E., Kin, Marta S., Martín, Kowalewski, Laitán, María G., Cecilia, Lanzone, Gabriel, Laufer, Carmen, Leizagoyen, Leonardi, María S., Rocío, Loizaga, Mapelli, Fernando J., Martin, Gabriel M., Guillermina, Massaccesi, Merani, María S., Julián, Mignino, Miño, Carolina I., Miotti, María D., Alejandro, Morici, Moschione, Flavio N., Mosti, Patricia A., Norberto, Muzzachiodi, Javier, Nori, Agustina, Novillo, Ojeda, Agustina A., Oklander, Luciana I., Orozco, María M., Ramiro, Ovejero, Palmerio, Andrés G., Paso Viola, María N., Hernán, Pastore, Paszko, Lorena M., Pérez Carusi, Lorena C., Perovic, Pablo G., Alejandro, Perretta, Pozzi, Carla M., Ramírez-Chaves, Héctor E., Relva, María A., Reppucci, Juan I., Facundo, Robino, Rodríguez-Cabal, Mariano A., Rodríguez-Planes, Lucía I., Ignacio, Roesler, Rossi, Luis F., Valentina, Segura, Claudio, Sillero-Zubiri, Florencia, Sittoni, Andrés, Tálamo, Torres Monges, Julio C., Alejandro, Travaini, Túnez, Juan I., Udrizar Sauthier, Daniel E., Uhart, Marcela M., Urquizo, José H., Vargas, Oscar E., Wiemeyer, Guillermo M., Marina, Winter, Wolfenson, Laura I., and Ricardo, Ojeda

Subjects
General Agricultural and Biological Sciences
Published
2023

11. Relationship Between Epileptic Activity and Developmental Outcome in KCNQ2-Related Epilepsy

Author

Dídac Casas-Alba, Anna Aguilar, Itziar Alonso, María Teresa García, Maria Roberta Cilio, Carmen Fons, Javier López-Pisón, Luis Gutiérrez-Solana, Fernando Ferragut, María Luz Ruiz-Falcó, Víctor Soto-Insuga, Elena González, Tamara Pablos, María José Mas, Sara Hernández, María Vázquez-López, Patricia Fuentes-Pita, Sergio Aguilera-Albesa, Rocío Sánchez-Carpintero, Montserrat Garcia-Puig, Deyanira García-Navas, Helena Alarcón-Martínez, Candelaria González, Rocío Calvo, Ana Extraviz, Jordi Muchart, Francesc Palau, Judith Armstrong, Dèlia Yubero, Carlos Eduardo Valera, Verónica González, Mar O'’Callaghan, Ariadna Borràs, Àngels García-Cazorla, Óscar Casis, Amaia Alquiza, Ainhoa Rodríguez de Yurre, and Álvaro Villarroel

Subjects
Developmental Neuroscience, Neurology, Pediatrics, Perinatology and Child Health, Neurology (clinical)
Published
2023

14. Genotoxic and cytotoxic evaluation of venlafaxine in an acute and a subchronic assay in mouse

Author

E. Madrigal-Bujaidar, P. Gómez-González, S. Camacho-Cantera, J. A. Morales-González, E. Madrigal-Santillán, and I. Álvarez-González

Subjects
Erythrocytes, Micronucleus Tests, antidepressant, Dose-Response Relationship, Drug, QH301-705.5, Science, Botany, Venlafaxine Hydrochloride, citotoxicidade, Antineoplastic Agents, ensaio in vivo, in vivo assay, Mice, QL1-991, QK1-989, micronuclei, Animals, cytotoxicity, antidepressivo, Biology (General), General Agricultural and Biological Sciences, Zoology, DNA Damage
Abstract

The present research was made to determine the micronuclei and cytotoxic capacity of the antidepressant venlafaxine in an in vivo acute and subchronic assays in mouse. In the first study, we administered once 5, 50, and 250 mg/kg of the drug, and included a negative and a daunorubicin treated group. Observations were daily made during four days. The subchronic assay lasted 5 weeks with daily administration of venlafaxine (1, 5, and 10 mg/kg) plus a negative and an imipramine administered groups. Observations were made each week. In the first assay results showed no micronucleated polychromatic erythrocytes (MNPE) increase, except with the high dose at 72 h. The strongest cytotoxic effect was found with 250 mg/kg at 72 h (a 51% cytotoxic effect in comparison with the mean control level). In the subchronic assay no MNPE increase was found; however, with the highest dose a significant increase of micronucleated normochromatic erythrocytes was observed in the last three weeks (a mean of 51% respect to the mean control value). A cytotoxic effect with the two high doses in the last two weeks was observed (a polychromatic erythrocyte mean decrease of 52% respect to the mean control value). Results suggest caution with venlafaxine. Resumo A presente pesquisa foi feita para determinar a capacidade micronuclei e citotóxica do antidepressivo venlafaxina em ensaios agudos e subcrônicos in vivo em camundongos. No primeiro estudo, administramos uma vez 5, 50 e 250 mg/kg do medicamento e incluímos um grupo negativo e um grupo tratado com daunorubicina. As observações foram feitas diariamente durante quatro dias. O ensaio subcrônico durou cinco semanas com administração diária de venlafaxina (1, 5, e 10 mg/kg) mais um grupo negativo e um grupo administrado de imipramina. As observações foram feitas a cada semana. No primeiro ensaio, os resultados não mostraram aumento de eritrócitos policromáticos micronucleados (MNPE), exceto com a dose elevada a 72 h. O efeito citotóxico mais forte foi encontrado com 250 mg/kg a 72 h (um efeito citotóxico de 51% em comparação com o nível médio de controle). No ensaio subcrônico não foi encontrado aumento de MNPE; entretanto, com a dose mais alta, um aumento significativo de eritrócitos normocromáticos micronucleados foi observado nas últimas três semanas (média de 51% em relação ao valor médio de controle). Foi observado um efeito citotóxico com as duas altas doses nas últimas duas semanas (uma diminuição média de 52% em relação ao valor médio de controle dos eritrócitos policromáticos). Os resultados sugerem cautela com a venlafaxina.

Published
2024

15. Primary non-gestational mediastinal choriocarcinoma metastatic to the brainstem

Author

Orlando De Jesus, Joel E Pellot Cestero, Frances M Gómez-González, and Román Vélez

Subjects
Male, Choriocarcinoma, Non-gestational, Mediastinum, General Medicine, Middle Aged, Mediastinal Neoplasms, female genital diseases and pregnancy complications, Pregnancy, embryonic structures, Humans, Female, Choriocarcinoma, reproductive and urinary physiology, Brain Stem
Abstract

Choriocarcinoma is a highly malignant tumour emerging from the syncytiotrophoblast divided into gestational and non-gestational presentations. Primary choriocarcinoma of the mediastinum is rare. Metastases to the brain often occur; however, brainstem involvement has not been reported for non-gestational choriocarcinoma. We described a middle-aged man who developed a complete left oculomotor nerve paralysis secondary to a brainstem tumour at the midbrain. The workup for the primary source of the brainstem tumour included a chest CT scan, which revealed a mediastinal mass. A mediastinal mass needle biopsy confirmed the diagnosis of primary mediastinal choriocarcinoma. Despite aggressive chemotherapy, the patient died 6 months after the initial presentation from neurological complications and multiorgan failure.

Published
2024

16. Control of SARS-CoV-2 Infection Rates at a Spanish University With In-Person Class Attendance

Author

Mario Gil-Conesa, Fares Amer, Silvia Carlos, Arturo H. Ariño, Miguel A. Martínez-González, and Alejandro Fernandez-Montero

Subjects
Universities, SARS-CoV-2, Communicable Disease Control, Public Health, Environmental and Occupational Health, COVID-19, Humans, Polymerase Chain Reaction
Abstract

The “Safe Campus Program,” implemented in 2020 through 2021 at the University of Navarra (Spain), aimed to guarantee a safe return to university campus and prevent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreaks, avoiding university-wide lockdown. It included COVID-19 education, campus adaptation, and polymerase chain reaction (PCR) testing. We describe the main characteristics of the program and analyze the SARS-CoV-2 cumulative incidence among 14 496 university members. The 14-day cumulative incidence in the university was 415.2 versus 447.7 in the region. The program, sustainable in the long term, achieved low SARS-CoV-2 in-campus rates. (Am J Public Health. 2022;112(4):570–573. https://doi.org/10.2105/AJPH.2021.306682

Published
2024

17. Colombia: avances y desafíos frente a la delincuencia organizada transnacional

Author

Sergio Stany Maldonado-Morales, Francisco Hersan González-Chavarro, Cristian David Ibáñez-Cano, Fabian Camilo Vargas-González, Edwin Andrés Rondón-Narváez, Cesar Enrique Ramos-Hinestrosa, Sandra Patricia Naizaque-Borda, Davinson Argemiro Mora-Carreño, and Mauro Andrés Martínez-Castillo

Abstract

A partir de la importancia que ha tomado la Delincuencia Organizada Transnacional actualmente, que se favorece por el proceso de globalización internacional, este libro busca abordar aquellas amenazas que en el marco de la seguridad multidimensional se categorizan en esta tipología criminal. En este sentido, el objetivo de esta investigación es analizar la incidencia de la Delincuencia Organizada Transnacional, tanto en la región como en Colombia. Esta investigación es un avance en la exploración de las amenazas a la seguridad que enfrentan las fronteras colombianas y trata temas de la coyuntura nacional e internacional, esperándose que sea un insumo de futuras investigaciones y proyecciones estatales en el abordaje de las amenazas de la Delincuencia Organizada Transnacional.

Published
2023

18. Effects of hospital facilities on patient outcomes after cancer surgery

Author

Knight, Sr, Chu, K, Lapitan, Mc, Dare, Aj, Pius, R, Shaw, Ca, Drake, Tm, Norman, L, Ademuyiwa, Ao, Adisa, Ao, Aguilera, Ml, Al-Saqqa, Sw, Al-Slaibi, I, Bhangu, A, Biccard, Bm, Brocklehurst, P, Costas-Chavarri, A, Elhadi, M, Fairfield, Cj, Fitzgerald, Je, Ghosh, D, Glasbey, J, Henegouwen, Miv, Ingabire, Jca, Kingham, Tp, Lawani, I, Lieske, B, Lilford, R, Martin, J, Mclean, Ka, Moore, R, Morton, D, Nepogodiev, D, Ntirenganya, F, Pata, F, Pinkney, T, Stephen, R Knight, Catherine, A Shaw, Riinu, Pius, Thomas, M Drake, Lisa, Norman, Adesoji, O Ademuyiwa, Adewale, O Adisa, Maria Lorena Aguilera, Sara, W Al-Saqqa, Ibrahim, Al-Slaibi, Aneel, Bhangu, Bruce, M Biccard, Peter, Brocklehurst, Ainhoa, Costas-Chavarri, Kathryn, Chu, Anna, Dare, Muhammed, Elhadi, Cameron, J Fairfield, J Edward Fitzgerald, Dhruv, Ghosh, James, Glasbey, Mark, I van Berge Henegouwen, C Allen Ingabire, J, T Peter Kingham, Marie Carmela Lapitan, Ismaïl, Lawani, Bettina, Lieske, Richard, Lilford, Janet, Martin, Kenneth, A McLean, Rachel, Moore, Dion, Morton, Dmitri, Nepogodiev, Faustin, Ntirenganya, Francesco, Pata, Thomas, Pinkney, Ahmad Uzair Qureshi, Antonio Ramos-De la Medina, Aya, Riad, Hosni Khairy Salem, Joana, Simões, Richard, Spence, Neil, Smart, Stephen, Tabiri, Hannah, Thomas, Thomas, G Weiser, Malcolm, West, John, Whitaker, Ewen, M Harrison, Arben, Gjata, Maria Marta Modolo, Sebastian, King, Erick, Chan, Sayeda Nazmun Nahar, Ade, Waterman, Dominique, Vervoort, Alemayehu Ginbo Bedada, Bernardo De Azevedo, Ana Gabriela Figueiredo, Manol, Sokolov, Venerand, Barendegere, Gerald, Ekwen, Arnav, Agarwal, Qinyang, Liu, Juan Camilo Correa, Kalisya Luc Malemo, Jacques, Bake, Jakov, Mihanovic, Kamila, Kunčarová, Julius, Orhalmi, Hosni, Salem, Jyri, Teras, Aristotelis, Kechagias, Alexis, P Arnaud, Judith, Lindert, Vasileios, Kalles, Maria-Lorena, Aguilera-Arevalo, Gustavo, Recinos, Zsolt, Baranyai, Basant, Kumar, Harish Neelamraju Lakshmi, Sanoop Koshy Zachariah, Philip, Alexander, Sunil Kumar Venkatappa, Pramesh, C, Radhian, Amandito, Christina, Fleming, Luca, Ansaloni, Gianluca, Pellino, Ahmed, M Altibi, Ibrahim, Nour, Intisar, Hamdun, Ali, M Ghellai, Donatas, Venskutonis, Tomas, Poskus, Justas, Zilinskas, Precious, Malemia, Yong Yong Tew, Elaine, Borg, Sarah, Ellul, Fatima Zahraa Wafqui, David, W Borowski, Anne Sophie van Dalen, Cameron, Wells, Harissou, Adamou, Adesoji, Ademuyiwa, Adewale, Adisa, Kjetil, Søreide, Sara Al Saqqa, Osaid, Alser, Haya, Tahboub, Helmut Alfredo Segovia Lohse, Sebastian Shu Yip, Piotr, Major, António Sampaio Soares, Matei Razvan Bratu, Andrey, Litvin, Armen, Vardanyan, Ahmad, Gudal, Naif, Albati, Jovan, Juloski, Miran, Rems, Sarah, Rayne, Stephanie Van Straten, Yoshan, Moodley, Irene Ortega Vázquez, Jaime, Ruiz-Tovar, Kithsiri Janakantha Senanayake, Sujeewa Priyantha Bandara Thalgaspitiya, Omer Abdelbagi Omer, Anmar, Homeida, Yucel, Cengiz, Daniel, Clerc, Muhammad, Alshaar, Hanen, Bouaziz, Yuksel, Altinel, Matthew, Doe, Maryna, Freigofer, Ella, Teasdale, Rakan, Kabariti, Joshua Michael Clements, Stephen Richard Knight, Ahsan, Ashfaq, Ijeoma, Azodo, Gabriela, Wagner, Ivan, Trostchansky, Mayaba, Maimbo, David, Linyama, Helidon, Nina, Amanda, Zeko, Claudio Gabriel Fermani, Santiago, Villalobos, Federico, Carballo, Pablo, Farina, Sebastian, Guckenheimer, Marilla, Dickfos, Ankit, Ajmera, Chester, Chong, Ralph, Gourlay, Sikandar, Hussaini, Yi Jia Lee, Adeeb, Majid, Peter, Martin, Rebecca, Miles, Owen James Morris, Jamie, Phua, William, Ridley, Tarunpreet, Saluja, Ryan Renxin Tan, Jen, Teh, Anna, Wells, Bharti, Arora, Qaasim, Dollie, Debbie, Ho, Yanru, Ma, Omattage Mahasha Perera, Anthony, Truong, Amanda Caroline Dawson, Bryan, Lim, Upuli, Pahalawatta, Jacqueline, Phan, Xiao-Ming Sarah Woon-Shoo-Tong, Andrea, Yeoh, Lillian, Charman, Andrew, Drane, Sharon, Laura, Charmaine Chu Wen Lo, Amy, Mozes, Rita, Poon, Hao Han Tan, Ellen, Wall, Prakshi, Chopra, Jasmine De Giovanni, Bal, Dhital, Brian, Draganic, Alexander, Duller, Jonathan, Gani, Yao Kuan Goh, Jun Young Jeong, Brendan, Mcmanus, Prakash, Nagappan, Peter, Pockney, Anya, Rugendyke, Mahsa, Sarrami, Stephen, Smith, Vanessa, Wills, Hsu Ven Wong, Geoffrey, Ye, Geoffrey, Zhang, Ethan, Brooker, Daniel, Feng, Bonnie, Lau, Carlin, Ngai, Sarah, Birks, David, Gyorki, Jaime Otero de Pablos, Ali, Abbosh, Chris, Gillespie, Ahmed, Mahmoud, Bianca, Kwan, Joshua, Lawson, Andrea, Warwick, Janne, Bingham, Andrew, J Cockbain, Nagendra Naidu Dudi-Venkata, Jordan, Ellaby-Hall, Ben, Finlay, Emily, Humphries, Jade, Pisaniello, Monique, Pisaniello, Salma, Salih, Tarik, Sammour, Haidar Hadri Abd Wahab, April De Silva, Nicola, Hayward, Kartik, Iyer, Guy, Maddern, Gian Andrea Prevost, Naga, Annapureddy, Krishna Pranathi Settipalli, Jeremy, Yeo, Lucy, Hempenstall, Lily, Pham, Shaun, Purcell, Cherry, Talavera, Ashish, I Vaska, Gurpreet, Chaggar, Phillip, Chrapko, Annelise, Cocco, Sarah Michelle Crystal Jade Coulter-Nile, Grahame, Ctercteko, James, French, Houchen, Gong, Martijn, Gosselink, Thuvarahan, Jegathees, Ivan, Jin, Michelle, Kalachov, Kathryn, Kiefhaber, Katherine, Lee, Jason, Luong, Steven, Phan, Henry, Pleass, Kelly, Veale, Zhi, Zeng, Angela, Au, Ashe, Debiasio, Idy, Deng, Jananee, Myooran, Amrita, Nair, Peter, Stewart, Anton, Stift, Lukas Walter Unger, Kerstin, Wimmer, Nabila, Ahmed, Syed, Hasan, Saber, Rahman, Margaret, O'Shea, Greg, Padmore, Adrian, Peters, Pietro, Perduca, Guenda, Pulcina, Nicolas, Tinton, Frederic, Buxant, Elsa, Dabin, Giulia, Garofalo, Francis, Dossou, Freddy Houehanou Rodrigue Gnangnon, Yacoubou Imorou Souaibou, Pako, Motlaleselelo, Omphile, Tlhomelang, Igor Lima Buarque, Gustavo Mendonça Ataíde Gomes, Aldo Vieira Barros, Ilia, Batashki, Nikolai, Damianov, Vladislav, Stoyanov, Dragomir, Dardanov, Svilen, Maslyankov, Plamen, Petkov, George, Todorov, Evgeni, Zhivkov, Aygulya, Akisheva, Miguel Angel Castilla Moreno, Geno, Genov, Ivelina, Ilieva, Tsvetomir, Ivanov, Martin, Karamanliev, Azhar, Khan, Emil, Mitkov, Tsanko, Yotsov, 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Ahmed Tarek, Muad Fathi Khalleefah Abu Hallalah, Saleh Abujamra, Hazem Abdelkarem Ahmed, Tagwa Alsharef, Abdulsalam Ali Ben Saoud, Tasnim El Gharmoul, Ahmed Elhadi, Safa Elrais, Abdulhalim Shebani, Heba Zarti, Asaid Zeiton, Marijus Ambrazevicius, Nerijus Kaselis, Migle Stakyte, Oleg Aliosin, Agne Cizauskaite, Sarunas Dailidenas, Vitalijus Eismontas, Migle Kybransiene, Vitalija Nutautiene, Narimantas Samalavicius, Dainius Simcikas, Algirdas Slepavicius, Albinas Tamosiunas, Nerijus Ubartas, Paulius Zeromskas, Saulius Bradulskis, Edvinas Dainius, Juozas Juočas, Egle Kubiliute, Juozas Kutkevičius, Aurimas Opolskis, Audrius Parseliunas, Andrejus Subocius, Donatas Venskutonis, Egle Virbickaite, Diana Zuikyte, Algirdas Bogusevicius, Kristina Buzaite, Daiva Čepuliené, Ieva Cesleviciene, Vaidotas Cesna, Jolanta Gribauskaite, Povilas Ignatavicius, Mantas Jokubauskas, Monika Liugailaitè, Ernest Margelis, Ruta Mazelyte, Lina Pankratjevaitè, Matas Pažusis, Agne Rackeviciute, Justina Saladyte, Monika Škimelytè, Vygintas Šlenfuktas, Monika Sudeikyte, Algimantas Tamelis, Tomas Vanagas, Žygimantas Žumbakys, Aivaras Atkociunas, Audrius Dulskas, Justas Kuliavas, Justas Birutis, Sigitas Paškevičius, Mindaugas Šatkauskas, Donatas Danys, Matas Jakubauskas, Lina Jakubauskiene, Marius Kryzauskas, Vytautas Lipnickas, Gabija Makūnaitè, Fanjandrainy Rasoaherinomenjanahary, Herizo Rasolofonarivo, Luc Hervé Samison, Bitiel Banda, Precious Malemia, Vanessa Msosa, Ahmad Imran Ahmad Izzuddin, Andre Das, Ying Yee Gan, Tan Shong Sheng, Jia Yng Siaw, Mohd Fadliyazid Ab Rahim, Dyg Zahratul Hamrak Abang Jamari, Nurfariza Che Husin, Muhd Yusairi Kamarulzaman, Yi Ping Lim, Nil Amri Mohamed Kamil, Mohd Razeen Mohd Hassan, Saidah Mohd Sahid, Johari Mustafa, Elaine Hui Been Ng, Wan Khamizar Wan Khazim, Ng Chang Ern, P G Lingeshan, Syariz Ezuan Sulaiman, Sue Ean Ang, Muhammad Navid Bin Mohamad Sithik, Yih Jeng Cheong, Mahadevan Deva Tata, Law Jia Xian, Aravinthan Kadravello, I-Ern Koh, Li-Yen Ng, Yuki 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S., Wells, C., Adamou, H., Ademuyiwa, A., Adisa, A., Soreide, K., Al Saqqa, S., Alser, O., Tahboub, H., Segovia Lohse, H. A., Shu Yip, S., Major, P., Sampaio Soares, A., Bratu, M. R., Litvin, A., Vardanyan, A., Allen Ingabire, J. C., Gudal, A., Albati, N., Juloski, J., Rems, M., Rayne, S., Van Straten, S., Moodley, Y., Ortega Vazquez, I., Ruiz-Tovar, J., Senanayake, K. J., Thalgaspitiya, S. P. B., Omer, O. A., Homeida, A., Cengiz, Y., Clerc, D., Alshaar, M., Bouaziz, H., Altinel, Y., Doe, M., Freigofer, M., Teasdale, E., Kabariti, R., Clements, J. M., Ashfaq, A., Azodo, I., Wagner, G., Trostchansky, I., Maimbo, M., Linyama, D., Nina, H., Zeko, A., Fermani, C. G., Villalobos, S., Carballo, F., Farina, P., Guckenheimer, S., Dickfos, M., Ajmera, A., Chong, C., Gourlay, R., Hussaini, S., Lee, Y. J., Majid, A., Martin, P., Miles, R., Morris, O. J., Phua, J., Ridley, W., Saluja, T., Tan, R. R., Teh, J., Wells, A., Arora, B., Dollie, Q., Ho, D., Ma, Y., Perera, O. M., Truong, A., Dawson, A. 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M., Mohsine, R., Rafik, A., Oung, T., Tin, M. M., Plarre, P., Alberga, A., Sluiter, N., Tuynman, J., Blok, R., Comert, D., Hompes, R., Kalff, M., Stellingwerf, M. E., Tanis, P., van Berge Henegouwen, M., van Praag, E. M., Wisselink, D., Gerhards, M., Lopes Cardozo, J., Westerduin, E., de Jonge, J., van Geloven, A., van Schilt, K., den Boer, F., Stoots, S., Vlek, S., Adams, J., Al-Busaidi, I. S., Budd, G., Choi, S. I., Chu, M. J. J., Ganugapati, A., Mckinstry, L., Pascoe, R., Richards, S., Rosser, K., Stevenson, A., White, R., Farik, S., Kwun, J., Murad, A., Cowan, S., Hall, T., Hayton, M., Malam Sani, L., Oumarou Garba, S., Amadou Magagi, I., Habou, O., Aliyu, H., Daniyan, M., Sholadoye, T. T., Abdullahi, L., Anyanwu, L. -J., Mohammad Mohammad, A., Muhammad, A. B., Sheshe, A. A., Suleiman, I., Adesina, A., Awolowo, A., Onuoha, C., Salami, O., Taiwo, O., Taiwo, A., Kache, S., Makama, J. 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B., Shahzad, N., Khaliq, T., Rashid, I., Waqar, S. H., Abu Al-saleem, H., Abu Alqumboz, A., Alqadi, M., Amro, A., Assa, R., Awesat, E., Ayyad, R., Hammad, M., Haymony, A., Hijazi, B., Hmeidat, B., Lahaseh, R., Qawasmi, A., Rajabi, A., Shehada, M., Shkokani, S., Yaghi, Y., Yaghi, N., Alzohour, M., Farid, M., Habes, Y. M., Juba, W., Nubani, Y., Rabee, A., Sa'Deh, M., Abed, S., Al basos, I., Alswerki, M., Ashour, D., Awad, I., Diab, S., El Jamassi, A., El-Kahlout, S., Elhout, S., Hajjaj, A. N. K., Hasanain, D., Nabil hajjaj, B., Obaid, M., Saikaly, E., Salhi, A., Al-Tammam, H., Almasri, M., Baniowda, M., Beshtawi, D., Horoub, A., Misk, R., Mohammad, B., Qasrawi, R., Sholi, T., Abu-Nimeh, S., Abu-srour, A., Abukhalaf, S. A., Adawi, S., Alsalameh, B., Ayesh, K., Elqadi, M., Hammouri, A., Karim Mustafa, F., Marzouqa, N., Melhem, S., Miqdad, D., Mohamad, B., Rawhi, M., Abu Ahammala, A. B., Abu Ataya, A., Abu Jayyab, I., Al-Shwaikh, S., Alagha, O., Alasttal, M., Awadallah, H., Elblbessy, M., Fares, J., Jarbou, A., Mahfouz, I., Albahnasawi, M. A., Abo mahadi, A., Abuelhatal, H., Abuelqomboz, A., Almoqayyad, A., Alwali, A., Balaawi, R., Hamouda, M., Humeid, M., Jedyan, A., Mahmoud Abu hamam, T., Matar, G., Salem, A., Samra, T., Shaheen, N., Shihada, K., A. Nemer, A., Abu Al Amrain, M., Abu Alamrain, A., Abu Jamie, N., Abu-Rous, M. R., Alfarra, N., Altaweel, M., Alwhaidi, N., Hamed, R., Saqqa, B., Shaheen, A., Aljaber, D., Aljaberi, L., Alwaheidi, M., Jawaada, A., Khaldi, H., Qahoush, R., Qari, J., Saadeh, R., Salim, A., Yacoub, A., Abbas, A., Abu shua'ib, R., Abu Zainah, B., Abusirrees, M., Babaa, B., Barhoush, O., Belal qadomi, A., Daraghmeh, L., Haji, R., Khatatbeh, A., Khatib, L., Qarariah, S., Quzmar, Y., Safadi, K., Salameh, R., Hassan, M., Herzallah, S., Massad, L., Nazzal, A., Nazzal, R., Escobar, D., Machain V, G. M., Rodriguez Gonzalez, A., Chachaima Mar, J. E., Chinchihualpa Paredes, N. O., Cuba, V., Lopez, W., Niquen Jimenez, M. M., Sanchez Bartra, N. A., Sapallanay Ojeda, O., Sequeiros, D., Toscano Pacheco, A., Vergara, M., Abarca, S., Alcorta, R., Borda-Luque, G., Eusebio Zegarra, I. E., Lujan Lopez, C., Marrufo, M., Mogrovejo, C., Nomura, A., Rodriguez Angeles, Y., Vidal Meza, M. R., Zavala, G., Castillo Arrascue, J. N., Hidrogo Cabrera, J. C., Larrea vera, J. J. M., Osorio, M., Ylatoma Diaz, E. A., Fontanilla, M. A., Fuentes, J. R., Salazar, A. L., Dominguez, G., Lopez, M. P., Macalindong, S., Onglao, M. A., Ramirez, A., Sacdalan, M. D., Tampo, M. M., Uy, G. L., Mangahas, J., Yabut, K., Canete, J. P., Cansana, B. E., Castro, E. J., Lipana, M. K., Roxas, M. F., Zara, V. 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M., Guimaraes, A., Marques, J., Nico Albano, M., Silva, M., Valente da Costa, A., Vieira Caroco, T., Almeida Braga, S., Capunge, I., Fragoso, M., Guimaraes, J., Pinto, B., Ribeiro, J., Angel, M., Fialho, G., Guerrero, M., Campos Costa, F., Cardoso, D., Cardoso, V., Alves, M., Estalagem, I., Louro, T., Marques, C., Martelo, R., Morgado, M., Canotilho, R., Correia, A. M., Martins, P., Peyroteo, M., Gomes, J., Monteiro, R., Romano, M., Alves, D. M., Peixoto, R., Quintela, C., Jervis, M. J., Melo, D., Pacheco, A., Paixao, V., Pedro, V., Pimenta, J., Pimenta de Castro, J., Rocha, A., Beuran, M., Ciubotaru, C., Diaconescu, B., Hostiuc, S., Negoi, I., Stoica, B., Anokhin, E., Kuznetsov, G., Oganezov, G., Paramzin, F., Romanova, E., Rutkovskii, V., Shushval, M., Zabiyaka, M., Dzhumabaev, K., Ivanov, V., Mamedli, Z., Achkasov, S., Balkarov, A., Nabiev, E., Nagudov, M., Rybakov, E., Saifutdinova, K., Sushkov, O., Joseph, L., Ndayishimiye, I., Faustin, N., Mutabazi, A. Z., Mvukiyehe, J. 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K., Rencuzogullari, A., Isik, A., Leventoglu, S., Yalcinkaya, A., Yuksel, O., Kalayci, M. U., Kara, Y., Sarici, I. S., Akin, A., Alemdag, G. N., Arslan, E., Baki, B. E., Bodur, M. S., Calik, A., Candas Altinbas, B., Cihanyurdu, I., Erkul, O., Gul, B., Guner, A., Kose, B., Semiz, A., Sevim, Tayar, S., Tomas, K., Tufek, O. Y., Turkyilmaz, S., Ulusahin, M., Usta, A., Yildirim, R., Guler, S. A., Tatar, O. C., Varol, E., Kirimtay, B., Uysal, M., Yildiz, A., Kose, E., Ciftci, A. B., Colak, E., Eraslan, H., Kucuk, G. O., Yemez, K., Lule, H., Bienfait, M., Bua, E., Okalany, N., Basarab, M., Bielosludtsev, O., Kolhanova, K., Perepelytsia, K., Romanukha, K., Savenkov, D., Siryi, S., Tereshchenko, M., Viacheslav, N., Volovetskyi, A., Kebkalo, A., Tryliskyy, Y., Tyselskiy, V., Bruce, E., Chow, B. L., Iddles, E., Mcguckin, S., Newall, N., Ramsay, G., Sharma, P., Stewart, C., Wong, J., Badran, A., Bath, M., Belais, F., Butt, E., Joshi, K., Kapur, M., Shaw, M., Townson, A., Williams, C. Y. K., Gray, T., Greig, R., Husain, M., Murray, E., Mustafa, A., Asif, A., Gokul, A., Shah, M., Akitikori, M. T., Charalabopoulos, A., Davidson, S., Mcnally, S., Rupani, S., Juma, F., Mills, S. C., Muirhead, L., Sellars, K., Walsh, U., Warren, O., Chambers, A., Hunt, R., Boyce, S., Cornwall, H., Tol, I., Argyriou, E. O., Eardley, N., Povey, M., Aithie, J. M. S., Irfan, A., Mcguigan, M. -C., Starr, R., Warren, C. R., Archibald, J., Kirby, G., Kisyov, I., Khoo, C. K., Lee, R., Photiou, D., Davis, R., Prasad, U., Yang, P. Z., Bird, J., Leung, E., Summerour, V., Currow, C., Kiam, J., Tan, G. J. S., Muthusami, A., Pegba-Otemolu, I., Urbonas, T., Nunoo-Mensah, J., Smolskas, E., Boddy, A., Gravante, G., Hunter, D., Andrew, D., Koh, A., Thompson, A., Adams, L., Clements, H. A., De Silva, K., Ekpete, O., Haque, S., Henderson, S., Ibrahim, B., Jayasinghe, T., Livie, J., Mailley, K., Nair, G., Tan, D., Baggaley, C., Dawidziuk, A., Szyszka, B., Barter, C., Gandhi, N., Hassell, K., Hitchin, S., Kelsall, J., Nagy, E., Nessa, A., Whisker, L., Yanni, F., Ali, M., Arora, D., Hediwattege, S., Kumarasinghe, N., Rathore, M., Tennakoon, A., Ali Ahmad, S. M., Bajomo, O., Nadira, F., Celentano, V., Griffiths, E., Karri, R. S., Mak, J. K. C., Pipe, M., Bhatti, M. I., Rabie, M., Boyle, C., Hamilton, D., Mihuna, A., Ng, J. C. K., Nicholson, G., Oliwa, A., Pearson, R., Rose, A., Yong, S. Q., Boereboom, C., Hanna, M., Walter, C., Greensmith, T. S., Mitchell, R., Monaghan, E., Crawford, J., Moug, S., Blackwell, J., Boyd-Carson, H., Herrod, P., Al-Allaf, O., Beattie, M., Bullock, C., Burman, S., Clark, G., Flamey, N., Flannery, O., Harding, A., Kodiatt, B., Lawday, S., Mahapatra, S., Mukundu Nagesh, N., Ng, M., Rye, D., Yoong, A., Clark, L., Deans, C., Edirisooriya, M., Carrington, E. V., Wong, T. L. E., Yusuf, B., Chamberlain, C., Duke, K., Kmiotek, E., Botes, A., Condie, N., Schrire, T., Shah, R., Thomas-Jones, I., Yates, C., Anthony, N., Matthews, E., Sahnan, K., Tankel, J., Tucker, S., Winter Beatty, J., Ziprin, P., Duggan, W., Kantartzi, A., Sridhar, S., Khaw, R. A., Srivastava, P., Underwood, C., Alves do Canto Brum, H., Chopra, S., Davis, L., Hughes, R., Tulley, J., Alberts, J., Athisayaraj, T., Olugbemi, M., Ahmad, K., Chan, C., Chapman, G., Fleming, H., Fox, B., Grewar, J., Hulse, K., Rutherford, D., Sinead, M., Speake, D., Vaughan-Shaw, P. G., Christodoulides, N., Kudhail, S., Welch, M., Husaini, S. M., Lambracos, S., Anyanwu, C., Suresh, R., Thomas, J. S., Gleeson, E., Platoff, R., Saif, A., Enumah, Z., Etchill, E., Gabre-Kidan, A., Bernstein, M., Carrano, F. M., Connors, J., Lynn, P., Melis, M., Newman, E., Foster, D. S., Perrone, K., Titan, A., Ahmad, S., Bafford, A. C. M. D., Dal Molin, M., Hanna, N., Zafar, S. N., Hemmila, M., Napolitano, L., Wong, J. J., Chandler, J., Wood, L., Wren, S., Ottesen, T., You, L., Yu, K., Arcienega Yanez, M. D. P., Ferreira Fernandes, M., Gonzalez, D., Cubas, S., Gonzalez, M. C., Zubiaurre, V., Demolin, R., Giroff, N., Sciuto, P., Campos, M., Rodriguez Cantera, G., Deepika, G., Simuchimba, E., Bulaya, A., Chibuye, C., Chirengendure, B., Kabale, M. -R., Kabongo, K., Munthali, J., Mweso, O., Pikiti, F., Otieno, J., Lai, L. T., Blackman, B., Subramaniam, S., Karim, R., Kok, N., Lee, Y. D., Ali, S., Sinha, A., Corrigan, R., Barnes, N., Wong, F., Dennis, G., Jedamzik, J., Phillips, E., Piette, W., Van hentenryck, M., Koco, H., Lawani, S., Kassa, M. W., Santos Bezerra, T., Gribnev, P., Dimitrov, D., Krastev, P., Oum, S., Bonghaseh, D. T., Al Farsi, M., Alsharqawi, N., Acevedo, V., Castillo Barbosa, A. C., Giron, F., Leon Rodriguez, J. P., Kucan, D., Rosko, D., Barsic, N., Zupan, D., Hegazi, A., Truncikova, V., Fryba, V., Mohamed, M., Nagi, A., Rashad Temerik, A., Elshawy, M. E., Mahmoud, M. I., Anwar, M., Rageh, T., Elmokadem, A., Gaballa, K., Teppo, S., Pengerma, P., Ballouhey, Q., Bergeat, D., Weyl, A., Hain, E., Gyedu, A., Yenli, E., Osei-Poku, D., Rompou, V. -A., Zoikas, A., Gaitanidis, A., Koukis, G., Perivoliotis, K., Tavlas, P., Galanos-Demiris, K., Zografos, G., Karavokyros, I., Xanthopoulou, G., Iordanidou, E., Ayau, F., Garcia, A., Damjan, P., Wason, D., B L, A., Rangganata, E., Kamath, P., O'Connor, D. B., Pinto, M., Perrone, F., Tropeano, F. P., Troilo, F., Bossi, D., Scala, D., Pulitano, L., Carella, M., Pietrabissa, A., Gori, A., Giraudo, G., De Simone, V., Russo, A. A., Braccio, B., Al-Taher, R., Athamneh, S., Parker, A., Sawiee, A., Kattia, A., Tababa, O., Shaeeb, Z., Syminas, V., Jurgaitis, J., Damuleviciene, G., Svagzdys, S., Razafimanjato, N. N. M., Chieng Loo, L., Tiong, I. C., Wan Muhmad, W. F., Vijeyan, H., Li Ying, T., Grech, G., Arrangoiz, R., Jimenez Ley, V. B., Arizpe, D., Lagunes Lara, E., Castro Lopez, E. V., Eaazim, J., Gordinou de Gouberville, M., Bastiaenen, V., Rottier, S., Nahab, F., Ji, M. Y., Seyoji, M., Nwachukwu, C., Emeghara, O., Muhammed, S. E., Idowu, A., Sowemimo, O., Ogundoyin, O., Akande, O., Lott, A., Nadeem, M., Laghari, A. A., Loya, A., Mushtaq, H., Abdullah, M. T., Abuhilal, B., Atawneh, M., Hamdan, H., Alhabil, B., Srour, A., Mousa, I., Da Silva Medina, L., Bartosiak, K., Ferreira, P., Francisco, V., Lemos, R., Frutuoso, L., Fernandes, S., Fonseca, T., Pereira, J., Rachadell, J., Torre, A., Madeira Martins, F., Carvalho, A. C., Rodrigues Ferreira, J., Ribeiro da Silva, B., Devesa, H., Vieira, A., Monica, I., Amaro, M., Sousa, D., Reia, M., Louro, J., Martins, A., Dominguez, J., Santos, I., Freitas Oliveira, N. M., Pereira, J. C., Silva-Vaz, P., Freire, L., Escrevente, R., Negoita, V. M., Shakhmatov, D., Nezerwa, Y., Radulovic, R., Obery, G., Viljoen, F., Mendes, T., Suarez, A., Moncada, E., Fernandez-Hevia, M., Curtis Martinez, C., Gil Garcia, J. M., Gonzalez Zunzarren, M., Idris, T., Eklov, K., Grahn, O., Amin, L., Blomqvist, M., Ajani, C., Kraus, R., Seeger, N., Willemin, M., Rayya, F., Ayash, M., Msouti, R., Kannas, I., Abazid, E., Esper, A., Slim, S., Kavcar, A. S., Aytac, E., Dural, A. C., Ilker, A., Eray, I. C., Kurnaz, E., Altiner, S., Tepe, M. D., Sahin, C., Savli, E., Innocent, A., Babirye, L., Diachenko, A., Hordoskiy, V., Curry, H., Chau, C. Y. C., Robertson, H., Lennon, H., Loi, L., Kirkham, E., Mccann, C., Watts, D., Gurung, B., Wilson, M., Tribedi, T., Garofalo, E., Zahra, B., Macdonald, S., Daniels, I., Ng, N., Khosla, S., Olivier, J., Yue, S. Y. P., Suresh, G., Wellington, J., Lorejo, E., Mossaad, M., Crutcher, M., Alimi, M., Baiu, I., Abdou, H., Conway, A., Peck, C., Perdomo Perez, M. A., Zulu, S., Nakazwe, M., Burger, S., Davies, J., Donaldson, R., Ede, C., Garden, O. J., Lesetedi, C., Mabedi, C., Magill, L., Makinde Alakaloko, F., Makupe, A., Monahan, M., Mulira, S., Muller, E., Musowoyo, J., Olory-Togbe, J. L., Roberts, T., Smith, M., Tayler, V., Windsor, J., Yepez, R., Sundar, S., Runigamugabo, E., Verjee, A., Chen, J., Daya, L., El Aroussi, N., Farina, V., Gnintedeme Olivier, T., Gonzales Nacarino, M., Hammani, A., Honjo, S., Jacobs, R., Kimura, H., Nkoronko, M., Oscullo Yepez, J. J., Pin Hung, W., Raj, A., Romani Pozo, A., Rommaneh, M., Sassamela Fabiano, S. C., Shiroma Gago, C. M., Srinivas, A., Sung, C. -Y., Tai, A., Valle Aranda, Y. C., Venturini, S., Wilguens Lartigue, J., Public and occupational health, AMS - Sports, APH - Health Behaviors & Chronic Diseases, CCA - Cancer Treatment and quality of life, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, and Albarella, Sara

Subjects
global surgery, hospitals, humans, income, neoplasms, postoperative period, poverty, prospective studies, no keywords listed, General Medicine, Prospective Studie, Hospital, Settore MED/18 - Chirurgia Generale, outcome, Hospitals, Humans, Income, Neoplasms, surgery, Postoperative Period, Poverty, Prospective Studies, cancer surgery, Human, hospital facilities
Abstract

© 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 licenseBackground: Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide. Methods: A multimethods analysis was performed as part of the GlobalSurg 3 study—a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital. Findings: Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3·85 [95% CI 2·58–5·75]; p

Published
2022

19. Off-label Use of Oral Immunotherapy for Rhinoconjunctivitis and Asthma due to Grass Pollen: A Safe and Effective Alternative in Patients over 65 Years Old: A Series of Case Reports

Author

Ana Rosado, Lucía González-Bravo, Jimena Laiseca García, and Martina Privitera

Subjects
Pharmacology, Pharmacology (medical), Toxicology
Abstract

Introduction: Allergic rhinoconjunctivitis and asthma are the most common IgE-mediated diseases worldwide. Allergen-specific immunotherapy (AIT) is currently the only modifying treatment for these IgE-mediated diseases in both children and adults. Subcutaneous immunotherapy is widely used, but in patients over 65 years old, there may be an increased risk of adverse reactions and a worse response to treatment. Oral immunotherapy (OIT) has been proven to be effective and safe, but currently, in most countries, it has been licensed only for patients up to 65 years old based on its technical datasheet. So far, no studies on the efficacy and safety of this type of immunotherapy in patients older than 65 years old have been published. Case Presentation: We present four patients older than 65 years old with a diagnosis of moderate seasonal rhinoconjunctivitis and moderate-persistent seasonal pollen-induced asthma. Off-label use of oral immunotherapy (OIT) for grass pollen was prescribed due to the severity of their rhinoconjunctivitis symptoms and the worsening of asthma symptoms during the spring. Improvement in the rhinoconjunctivitis and asthma symptoms was reported by all patients since the first spring season and was maintained during the following two years of follow-up. There were no systemic reactions, and only two patients initially had self-limiting oral pruritus. Conclusion: Oral immunotherapy for pollens appears to be a convenient, effective, and safe option in older patients (>65 years) with comorbidities after a three-year treatment. This is, to the best of our knowledge, the first report on the off-label use of OIT in patients over 65 years old with symptoms of allergic rhinoconjunctivitis and asthma.

Published
2023

22. Differential Neurobiological Markers in Phenotype-stratified Rats Modeling High or Low Vulnerability to Compulsive Behavior: A Narrative Review

Author

Margarita Moreno- Montoya, Elena Martín-González, Manuela Olmedo-Córdoba, and Pilar Flores

Subjects
Pharmacology, Psychiatry and Mental health, Neurology, Pharmacology (medical), Neurology (clinical), General Medicine
Abstract

Abstracts: Compulsivity is a key manifestation of inhibitory control deficit and a cardinal symptom in different neuropsychopathological disorders such as obsessive-compulsive disorder, schizophrenia, addiction, and attention-deficit hyperactivity disorder. Schedule-induced polydipsia (SIP), is an animal model to study compulsivity. In this procedure, rodents develop excessive and persistent drinking behavior under different food-reinforcement schedules, that are not related to homeostatic or regulatory requirements. However, there are important individual differences that support the role of high-drinker HD rats as a compulsive phenotype, characterized in different paradigms by inhibitory response deficit, cognitive inflexibility, and resistant to extinction behavior; with significant differences in response to pharmacological challenges, and relevant neurobiological alterations in comparison with the control group, the non-compulsive low drinker LD group on SIP. The purpose of this review is to collate and update the main findings on the neurobiological bases of compulsivity using the SIP model. Specifically, we reviewed preclinical studies on SIP, that have assessed the effects of serotonergic, dopaminergic, and glutamatergic drugs; leading to the description of the neurobiological markers, such as the key role of the serotonin 5-HT2A receptor and glutamatergic signaling in a phenotype vulnerable to compulsivity as high drinker HD rats selected by SIP. The review of the main findings of HD rats on SIP helps in the characterization of the preclinical compulsive phenotype, disentangles the underlying neurobiological, and points toward genetic hallmarks concerning the vulnerability to compulsivity.

Published
2023

25. Novel Nanotechnological Strategies for Skin Anti-aging

Author

Clara Luisa Domínguez-Delgado, Sara Pozos-Nonato, Kenia Areli Campos-Santander, Allyson Amelia Benavides, Sandy María Pacheco-Ortin, Rosa Isabel Higuera-Piedrahita, Guillermo Resendiz-González, and Eva María Molina-Trinidad

Subjects
Pharmaceutical Science, Biotechnology
Abstract

Background: Nanoparticle formulations development for anti-aging treatment is increasing due to their multifunctional properties. These nanotechnological strategies can target cellular/ molecular pathways of the skin affected by the aging process. However, a review of these strategies is required to discuss their efficacy/safety and establish the needs for further research. Objective: Innovative nanotechnological advances for skin anti-aging/rejuvenation are summarized and discussed in this work. Method: The information in this review was extracted from recent and relevant studies using nanotechnology for anti-aging treatment from scientific databases. Results and Discussion: Results show an enhanced skin anti-aging effect of actives-loaded nanoparticles of next generation (nanostructured lipid carriers, fullerenes, transfersomes, protransfersomes, niosomes, ethosomes, transethosomes, glycerosomes, phytosomes) compared with nanocarriers of first generation or conventional formulations. Anti-aging active ingredients such as, flavonoids (rutin, hesperidin, quercetagetine, quercetin, epigallocatechin-3-gallate, myricetin, silibinin, curcuminoids, isoflavones); vitamins (E, D3, CoQ10); acids (hyaluronic, ascorbic, rosmarinic, gallic); extracts (Citrus sinensis, Tagetes erecta L., Achillea millefolium L., Citrus aurantium L., Glycyrrhiza glabra L., Aloe vera, propolis earned by Apis mellifera); and other compounds (adenosine, beta-glucan, heptapetide DEETGEF, resveratrol, cycloastragenol, melatonin, botulinum toxin, grapeseed oil), have been successfully entrapped into nanoparticles for skin rejuvenation. This encapsulation has improved their solubility, bioavailability, stability, permeability, and effectivity for skin anti-aging, providing a controlled drug release with minimized side effects. Conclusion: Recent studies show a trend of anti-aging herbal active ingredients-loaded nanoparticles, enhancing the moisturizing, antioxidant, regenerating and photoprotective activity of the skin. Suitable safety/shelf-life stability of these novel formulations is key to a successful translation to the clinic/industry.

Published
2023

26. Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers

Author

Cosimo Posth, He Yu, Ayshin Ghalichi, Hélène Rougier, Isabelle Crevecoeur, Yilei Huang, Harald Ringbauer, Adam B. Rohrlach, Kathrin Nägele, Vanessa Villalba-Mouco, Rita Radzeviciute, Tiago Ferraz, Alexander Stoessel, Rezeda Tukhbatova, Dorothée G. Drucker, Martina Lari, Alessandra Modi, Stefania Vai, Tina Saupe, Christiana L. Scheib, Giulio Catalano, Luca Pagani, Sahra Talamo, Helen Fewlass, Laurent Klaric, André Morala, Mathieu Rué, Stéphane Madelaine, Laurent Crépin, Jean-Baptiste Caverne, Emmy Bocaege, Stefano Ricci, Francesco Boschin, Priscilla Bayle, Bruno Maureille, Foni Le Brun-Ricalens, Jean-Guillaume Bordes, Gregorio Oxilia, Eugenio Bortolini, Olivier Bignon-Lau, Grégory Debout, Michel Orliac, Antoine Zazzo, Vitale Sparacello, Elisabetta Starnini, Luca Sineo, Johannes van der Plicht, Laure Pecqueur, Gildas Merceron, Géraldine Garcia, Jean-Michel Leuvrey, Coralie Bay Garcia, Asier Gómez-Olivencia, Marta Połtowicz-Bobak, Dariusz Bobak, Mona Le Luyer, Paul Storm, Claudia Hoffmann, Jacek Kabaciński, Tatiana Filimonova, Svetlana Shnaider, Natalia Berezina, Borja González-Rabanal, Manuel R. González Morales, Ana B. Marín-Arroyo, Belén López, Carmen Alonso-Llamazares, Annamaria Ronchitelli, Caroline Polet, Ivan Jadin, Nicolas Cauwe, Joaquim Soler, Neus Coromina, Isaac Rufí, Richard Cottiaux, Geoffrey Clark, Lawrence G. Straus, Marie-Anne Julien, Silvia Renhart, Dorothea Talaa, Stefano Benazzi, Matteo Romandini, Luc Amkreutz, Hervé Bocherens, Christoph Wißing, Sébastien Villotte, Javier Fernández-López de Pablo, Magdalena Gómez-Puche, Marco Aurelio Esquembre-Bebia, Pierre Bodu, Liesbeth Smits, Bénédicte Souffi, Rimantas Jankauskas, Justina Kozakaitė, Christophe Cupillard, Hartmut Benthien, Kurt Wehrberger, Ralf W. Schmitz, Susanne C. Feine, Tim Schüler, Corinne Thevenet, Dan Grigorescu, Friedrich Lüth, Andreas Kotula, Henny Piezonka, Franz Schopper, Jiří Svoboda, Sandra Sázelová, Andrey Chizhevsky, Aleksandr Khokhlov, Nicholas J. Conard, Frédérique Valentin, Katerina Harvati, Patrick Semal, Bettina Jungklaus, Alexander Suvorov, Rick Schulting, Vyacheslav Moiseyev, Kristiina Mannermaa, Alexandra Buzhilova, Thomas Terberger, David Caramelli, Eveline Altena, Wolfgang Haak, Johannes Krause, Helsinki Institute of Sustainability Science (HELSUS), Department of Cultures, Faculty Common Matters (Faculty of Arts), Universidad de Cantabria, Posth, Cosimo [0000-0002-8206-3907], Yu, He [0000-0003-1323-4730], Rougier, Hélène [0000-0003-0358-0285], Ringbauer, Harald [0000-0002-4884-9682], Rohrlach, Adam B [0000-0002-4204-5018], Nägele, Kathrin [0000-0003-3861-8677], Villalba-Mouco, Vanessa [0000-0002-9357-5238], Radzeviciute, Rita [0000-0002-5800-3787], Stoessel, Alexander [0000-0003-2434-2542], Drucker, Dorothée G [0000-0003-0854-4371], Lari, Martina [0000-0002-7832-8212], Modi, Alessandra [0000-0001-9514-9868], Vai, Stefania [0000-0003-3844-5147], Scheib, Christiana L [0000-0003-4158-8296], Rué, Mathieu [0000-0001-7948-9459], Boschin, Francesco [0000-0001-5795-9050], Maureille, Bruno [0000-0002-7616-0073], Bortolini, Eugenio [0000-0001-6751-5680], Starnini, Elisabetta [0000-0002-3933-0854], Sineo, Luca [0000-0001-8634-2295], Garcia, Géraldine [0000-0001-5777-7126], Połtowicz-Bobak, Marta [0000-0003-1973-4971], Bobak, Dariusz [0000-0002-5216-6630], Le Luyer, Mona [0000-0001-7999-0294], Kabaciński, Jacek [0000-0002-2118-2005], Berezina, Natalia [0000-0001-5704-9153], González-Rabanal, Borja [0000-0002-1802-994X], Amkreutz, Luc [0000-0003-4664-5552], Bocherens, Hervé [0000-0002-0494-0126], Jankauskas, Rimantas [0000-0001-7611-2576], Conard, Nicholas J [0000-0002-4633-0385], Valentin, Frédérique [0000-0002-0575-7681], Harvati, Katerina [0000-0001-5998-4794], Schulting, Rick [0000-0002-4444-766X], Mannermaa, Kristiina [0000-0002-8510-1120], Buzhilova, Alexandra [0000-0001-6398-2177], Caramelli, David [0000-0001-6468-1675], Altena, Eveline [0000-0001-8911-7771], Haak, Wolfgang [0000-0003-2475-2007], Krause, Johannes [0000-0001-9144-3920], Apollo - University of Cambridge Repository, Rohrlach, Adam B. [0000-0002-4204-5018], Drucker, Dorothée G. [0000-0003-0854-4371], Scheib, Christiana L. [0000-0003-4158-8296], Conard, Nicholas J. [0000-0002-4633-0385], Posth, Cosimo, Yu, He, Ghalichi, Ayshin, Rougier, Hélène, Crevecoeur, Isabelle, Huang, Yilei, Ringbauer, Harald, Rohrlach, Adam B, Nägele, Kathrin, Villalba-Mouco, Vanessa, Radzeviciute, Rita, Ferraz, Tiago, Stoessel, Alexander, Tukhbatova, Rezeda, Drucker, Dorothée G, Lari, Martina, Modi, Alessandra, Vai, Stefania, Saupe, Tina, Scheib, Christiana L, Catalano, Giulio, Pagani, Luca, Talamo, Sahra, Fewlass, Helen, Klaric, Laurent, Morala, André, Rué, Mathieu, Madelaine, Stéphane, Crépin, Laurent, Caverne, Jean-Baptiste, Bocaege, Emmy, Ricci, Stefano, Boschin, Francesco, Bayle, Priscilla, Maureille, Bruno, Le Brun-Ricalens, Foni, Bordes, Jean-Guillaume, Oxilia, Gregorio, Bortolini, Eugenio, Bignon-Lau, Olivier, Debout, Grégory, Orliac, Michel, Zazzo, Antoine, Sparacello, Vitale, Starnini, Elisabetta, Sineo, Luca, van der Plicht, Johanne, Pecqueur, Laure, Merceron, Gilda, Garcia, Géraldine, Leuvrey, Jean-Michel, Garcia, Coralie Bay, Gómez-Olivencia, Asier, Połtowicz-Bobak, Marta, Bobak, Dariusz, Le Luyer, Mona, Storm, Paul, Hoffmann, Claudia, Kabaciński, Jacek, Filimonova, Tatiana, Shnaider, Svetlana, Berezina, Natalia, González-Rabanal, Borja, González Morales, Manuel R, Marín-Arroyo, Ana B, López, Belén, Alonso-Llamazares, Carmen, Ronchitelli, Annamaria, Polet, Caroline, Jadin, Ivan, Cauwe, Nicola, Soler, Joaquim, Coromina, Neu, Rufí, Isaac, Cottiaux, Richard, Clark, Geoffrey, Straus, Lawrence G, Julien, Marie-Anne, Renhart, Silvia, Talaa, Dorothea, Benazzi, Stefano, Romandini, Matteo, Amkreutz, Luc, Bocherens, Hervé, Wißing, Christoph, Villotte, Sébastien, de Pablo, Javier Fernández-López, Gómez-Puche, Magdalena, Esquembre-Bebia, Marco Aurelio, Bodu, Pierre, Smits, Liesbeth, Souffi, Bénédicte, Jankauskas, Rimanta, Kozakaitė, Justina, Cupillard, Christophe, Benthien, Hartmut, Wehrberger, Kurt, Schmitz, Ralf W, Feine, Susanne C, Schüler, Tim, Thevenet, Corinne, Grigorescu, Dan, Lüth, Friedrich, Kotula, Andrea, Piezonka, Henny, Schopper, Franz, Svoboda, Jiří, Sázelová, Sandra, Chizhevsky, Andrey, Khokhlov, Aleksandr, Conard, Nicholas J, Valentin, Frédérique, Harvati, Katerina, Semal, Patrick, Jungklaus, Bettina, Suvorov, Alexander, Schulting, Rick, Moiseyev, Vyacheslav, Mannermaa, Kristiina, Buzhilova, Alexandra, Terberger, Thoma, Caramelli, David, Altena, Eveline, Haak, Wolfgang, Krause, Johannes, Universidad de Alicante. Instituto Universitario de Investigación en Arqueología y Patrimonio Histórico, Prehistoria y Protohistoria, Pagani, Luca [0000-0002-6639-524X], and Alonso-Llamazares, Carmen [0000-0002-1053-1388]

Subjects
History, Ancient dna, Interactions, Cave, 45/23, Admixture, Settore BIO/08 - Antropologia, 631/208/457, 631/181/27, 6160 Other humanities, Contamination, Humans, Hunting, Palaeogenomics, Population-structure, Archaeology, Biological anthropology, Evolutionary genetics, Population genetics, History, Ancient, Human evolution, Diversity, Occupation, Multidisciplinary, 45, Genome, Human, article, Paleontology, Last glacial maximum, Human Genetics, Gene Pool, Genomics, 631/181/19/2471, Pleistocene, Europe, Genomic transformations, 631/181/2474, Anthropology, Hunter-gatherers, Genome sequence
Abstract

Acknowledgements: The authors thank G. Marciani and O. Jöris for comments on archaeology; C. Jeong, M. Spyrou and K. Prüfer for comments on genetics; M. O’Reilly for graphical support for Fig. 5 and Extended Data Fig. 9; the entire IT and laboratory teams at the Department of Archaeogenetics of MPI-SHH for technical assistance; M. Meyer and S. Nagel for support with single-stranded library preparation; K. Post, P. van Es, J. Glimmerveen, M. Medendorp, M. Sier, S. Dikstra, M. Dikstra, R. van Eerden, D. Duineveld and A. Hoekman for providing access to human specimens from the North Sea (The Netherlands); M. D. Garralda and A. Estalrrich for providing access to human specimens from La Riera (Spain); J. Górski and M. Zając for providing access to human specimens from Maszycka cave; C. Di Patti for providing access to human specimens from San Teodoro 2 (Italy); P. Blaževičius for providing access to the Donkalnis human remains and the new radiocarbon dates; the Italian Ministry of Culture and Soprintendenza Archeologia Belle Arti e Paesaggio for the Provinces of Verona, Rovigo, and Vicenza for granting access to the human remains of Tagliente 2; F. Fontana, who carries out investigations of the Riparo Tagliente site (Italy); the Friuli Venezia Giulia Superintendency for providing access to the human tooth Pradis 1; and the Soprintendenza Archeologia Belle Arti e Paesaggio for the Provinces of Barletta-Andria-Trani and Foggia for providing access to the Paglicci human remains. This project has received funding by the European Research Council under the European Union’s Horizon 2020 research and innovation programme under grant agreements no. 803147-RESOLUTION (to S.T.), no. 771234-PALEoRIDER (to W.H.), no. 864358 (to K.M.), no. 724703 and no. 101019659 (to K.H.). K.H. is also supported by the Deutsche Forschungsgemeinschaft (DFG FOR 2237). E.A. has received funding from the Van de Kamp fonds. PACEA co-authors of this research benefited from the scientific framework of the University of Bordeaux’s IdEx Investments for the Future programme/GPR Human Past. A.G.-O. is supported by a Ramón y Cajal fellowship (RYC-2017-22558). L. Sineo, M.L. and D.C. have received funding from the Italian Ministry of University and Research (MUR) PRIN 2017 grants 20177PJ9XF and 20174BTC4R_002. H. Rougier received support from the College of Social and Behavioral Sciences of CSUN and the CSUN Competition for RSCA Awards. C.L.S. and T. Saupe received support from the European Union through the European Regional Development Fund (project no. 2014-2020.4.01.16-0030) and C.L.S. received support from the Estonian Research Council grant PUT (PRG243). S. Shnaider received support from the Russian Science Foundation (no. 19-78-10053)., Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.

Published
2023
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27. Identidad de género y orientación sexual de las personas con Trastorno del Espectro del Autismo: una revisión sistemática

Author

Sara González-García, Patricia Solís-García, Lucía Morán-Suárez, Asunción Monsalve-González, Laura E. Gómez-Sánchez, and Ángeles Alcedo-Rodríguez

Subjects
Psychiatry and Mental health, Social Psychology, Pshychiatric Mental Health, Education
Abstract

En los últimos años ha surgido un creciente interés en relación con la identidad de género y la orientación sexual de las personas con trastorno del espectro del autismo (TEA). La presente revisión sistemática analiza un total de 18 estudios, publicados a partir de 2016, con la finalidad de obtener evidencias acerca de la identidad y disforia de género, y la orientación sexual en la población con TEA. Los resultados indicaron que existe una mayor prevalencia de identidad de género no conforme y de disforia de género en la población con TEA. Se encontró una mayor prevalencia de orientación no heterosexual en las personas con TEA en comparación con la población sin TEA. Estos resultados se encuentran preferentemente en las mujeres con TEA. No obstante, es necesario disponer de más y mejores evidencias sobre esta temática, especialmente en mujeres, y en las etapas de la niñez y la adolescencia, con el fin de implementar apoyos y recursos adecuados para dar respuesta a las necesidades de estas personas.

Published
2023

28. Percutaneous transient occlusion of the transtricuspid flow: a new method to evaluate the right ventricle-dependent coronary circulation in pulmonary atresia with intact ventricular septum

Author

José Luis Colín Ortiz, Sylvia K. Sánchez González, Bobadilla Aguirre Alfredo, González-Rebeles Guerrero Carlos, and Corona Villalobos Carlos Alfonso

Subjects
Pediatrics, Perinatology and Child Health, General Medicine, Cardiology and Cardiovascular Medicine
Abstract

Pulmonary atresia with an intact ventricular septum is characterised by heterogeneity in right ventricle morphology and coronary anatomy. In some cases, the presence of ventriculocoronary connections may promote coronary artery stenosis or interruption, and aortic diastolic pressure may not be sufficient to drive coronary blood flow. This requires a correct evaluation (currently done by angiography) which depends on whether the patient can be offered decompression of the right ventricle. To date, there is no objective method to do so, so we designed a percutaneous, transitory technique with the purpose of occluding the transtricuspid anterograde flow. The manoeuverer was performed in a 25-day-old female with pulmonary atresia with intact ventricular septum, right ventricle at suprasystemic level, and selective coronarography was not conclusive, the anterior descendant with stenosis in its middle third and from this point, thinner with to-fro flow. Occlusion was performed with a balloon catheter. We re-evaluated the coronary flow and the normalised anterior descendant flow. We hope that with this new method, we can give a more accurate diagnosis and determine the cases in which the coronary circulation is truly not right ventricle dependent to offer a greater number of patients biventricular or 1.5 ventricular repairs and thereby improve their quality of life and survival, the ones that turn out to be right ventricular dependant; offer them an early reference for cardiac transplant or in case it is not available to consider univentricular palliation knowing that this probably would not reduce the risk of ischaemia and/or death over time.

Published
2023

30. The Multifactorial Etiopathogenesis of Alzheimer’s Disease: Neuroinflammation as the Major Contributor

Author

Andrea González, Camila Calfio, Valentina Lüttges, Antonia González-Madrid, and Cristian Guzmán

Subjects
Psychiatry and Mental health, Clinical Psychology, General Neuroscience, General Medicine, Geriatrics and Gerontology
Abstract

Alzheimer’s disease (AD) is the most common form of dementia in the elderly. AD is a multifactorial disease, affected by several factors including amyloid-β42 oligomers, self-assembled tau, microbiota molecules, etc. However, inflammatory components are critical to trigger AD. Neuroinflammatory pathology links glial activation by “damage signals” with tau hyperphosphorylation, as explained by the Neuroimmunomodulation Theory, discovered by the ICC laboratory. This theory elucidates the onset and progression of several degenerative diseases and concept of “multitarget” therapy. These studies led to the rationale to identify inflammatory targets for the action of bioactive molecules or drugs against AD.

Published
2023

31. Análisis de un protocolo de radioterapia guiada por imagen con marcadores fiduciales en radioterapia prostática hipofraccionada

Author

Roser Fayos-Solá Capilla, Pablo Castro Tejero, Almudena Zapatero Laborda, David Hernández González, María Roch González, Alberto Viñals Muñoz, and Paloma Botella Faus

Subjects
Ocean Engineering, Safety, Risk, Reliability and Quality
Abstract

El objetivo principal de este trabajo es el evaluar la validez de un protocolo de radioterapia guiada por imagen (IGRT) implementado en casos de cáncer de próstata con implantación de marcadores fiduciales, así como caracterizar la magnitud de los desplazamientos intrafracción durante las cinco primeras sesiones de un grupo de 32 pacientes tratados a lo largo de un año. Dos semanas antes de la simulación se le implanta al paciente un mínimo de 3 marcadores fiduciales de oro intraprostáticos. Durante el tratamiento, con el uso combinado del sistema Auto Beam Hold (ABH) y la adquisición de imágenes pareadas kV-MV, se analiza el correcto posicionamiento de la zona a tratar y del paciente. De los resultados obtenidos, encontramos que ciertos pacientes acumulan la mayor parte de las interrupciones, lo que apunta a aquéllos que tendrían problemas en el posicionamiento desde el inicio y nos permite valorar la ampliación del seguimiento en determinados casos. Los resultados de los desplazamientos indican que todos los casos requieren correcciones menores de 5 mm. El uso de protocolos de radioterapia guiada por imagen (IGRT) en casos de anatomías variables, representa una mejora en el correcto posicionamiento del paciente y, por consiguiente, en la administración del tratamiento.

Published
2023

32. Vegetales de México con propiedades antidiabéticas

Author

Rafael German Campos Montiel, Uriel González Lemus, Alma Delia Hernández Fuentes, Lucio González Montiel, Maribel López Tolentino, and Ana Karen Zaldivar Ortega

Subjects
Complementary and alternative medicine, Pharmaceutical Science, Pharmacology (medical)
Abstract

En México algunos vegetales tienen propiedades medicinales y se han utilizado desde tiempos prehispánicos. Debido al incremento personas con diabetes mellitus (DM) surge la necesidad de desarrollar estrategias para la prevención del desarrollo y el tratamiento de esta enfermedad mediante productos naturales. En los mercados mexicanos existen diferentes especias y vegetales como pimienta, tomillo, ajo y cebolla, todos estos se caracterizan por poseer compuestos bioactivos capaces de intervenir en procesos biológicos como la reducción de la resistencia a la insulina, estimulación de la glucogénesis e inhibición de la enzima α-glucosidasa. Es importante estudiar la composición química de estos vegetales mexicanos y evaluar su efecto sobre la DM.

Published
2023

33. Desarrollo y evaluación de un sensor portátil para la detección de Hg2+, a través de la síntesis verde de AuNP utilizando extracto acuoso de Sargassum spp

Author

Miriam Rocío Estevez-González, Rodrigo Alonso Esparza Muñoz, Marlen Alexis González-Reyna, José Luis López-Miranda, and Gustavo Andrés Molina Labastida

Subjects
Cultural Studies, History, Literature and Literary Theory
Abstract

El presente trabajo tiene la finalidad de dar a conocer el desarrollo de un prototipo de sensor para la medición de mercurio (Hg2+) en solución acuosa sobre una plataforma electroquímica basada en electrodos serigrafiados de nanotubos de carbonos (CNT-SPE) en conjunto con nanopartículas de oro (NPs-Au) realizadas a través de síntesis verde, utilizando extracto acuoso de Sargassum spp. Para esto, se realizó la caracterización de las NPs-Au por espectroscopía ultravioleta-visible (UV-Vis), infrarrojo (TF-IR), difracción de rayos X (DRX) y microscopía electrónica de barrido (SEM); obteniendo nanopartículas entre 80-100 nm las cuales poseen un recubrimiento orgánico que les confiere una sinergia al momento de ser conjugadas con CNT-SPE oxidadas/activadas. Posteriormente, las NPs-Au se utilizaron para modificar el CNT-SPE a través del método drop-casting y así ensamblar un novedoso sensor electroquímico para la medición de Hg2+, cuya validación y habilidad para medir fue demostrada utilizando voltametría diferencial de pulso (DPV). Se obtuvieron límites de detección de 2.38 uM y cuantificación de 3 uM. Los resultados demuestran que la plataforma desarrollada es una alternativa rápida, eficiente y portátil para la medición de Hg2+.

Published
2023

34. Assessment of Purity, Functionality, Stability, and Lipid Composition of Cyclofos-nAChR-Detergent Complexes from Torpedo californica Using Lipid Matrix and Macroscopic Electrophysiology

Author

Orestes Quesada, Joel E. González-Nieves, José Colón, Rafael Maldonado-Hernández, Carol González-Freire, Jesús Acevedo-Cintrón, Irvin D. Rosado-Millán, and José A. Lasalde-Dominicci

Subjects
Physiology, Biophysics, Cell Biology
Abstract

The main objective of the present study was to find detergents that can maintain the functionality and stability of the Torpedo californica nicotinic acetylcholine receptor (Tc-nAChR). We examined the functionality, stability, and purity analysis of affinity-purified Tc-nAChR solubilized in detergents from the Cyclofos (CF) family [cyclofoscholine 4 (CF-4), cyclofoscholine 6 (CF-6), and cyclofloscholine 7 (CF-7)]. The functionality of the CF-Tc-nAChR-detergent complex (DC) was evaluated using the Two Electrode Voltage Clamp (TEVC) method. To assess stability, we used the florescence recovery after photobleaching (FRAP) in Lipidic Cubic Phase (LCP) methodology. We also performed a lipidomic analysis using Ultra-Performance Liquid Chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI–MS/MS) to evaluate the lipid composition of the CF-Tc-nAChR-DCs. The CF-4-Tc-nAChR-DC displayed a robust macroscopic current (− 200 ± 60 nA); however, the CF-6-Tc-nAChR-DC and CF-7-Tc-nAChR-DC displayed significant reductions in the macroscopic currents. The CF-6-Tc-nAChR and CF-4-Tc-nAChR displayed higher fractional florescence recovery. Addition of cholesterol produced a mild enhancement of the mobile fraction on the CF-6-Tc-nAChR. The lipidomic analysis revealed that the CF-7-Tc-nAChR-DC displayed substantial delipidation, consistent with the lack of stability and functional response of this complex. Although the CF-6-nAChR-DC complex retained the largest amount of lipids, it showed a loss of six lipid species [SM(d16:1/18:0); PC(18:2/14:1); PC(14:0/18:1); PC(16:0/18:1); PC(20:5/20:4), and PC(20:4/20:5)] that are present in the CF-4-nAChR-DC. Overall, the CF-4-nAChR displayed robust functionality, significant stability, and the best purity among the three CF detergents; therefore, CF-4 is a suitable candidate to prepare Tc-nAChR crystals for structural studies. Graphical abstract

Published
2023

35. Sexismo en estudiantes de educación física de Chile (Sexism in physical education students in Chile)

Author

Fernando Maureira Cid, Elizabeth Flores Ferro, Jessica Ibarra Mora, Patricia González Flores, Marcelo Barrera Bolados, Cristian Cuevas González, Joaquín Matamala Muñoz, and Francisca Sepúlveda Ormeño

Subjects
Orthopedics and Sports Medicine, Physical Therapy, Sports Therapy and Rehabilitation, Education
Abstract

El sexismo corresponde a un conjunto de creencias de las características que poseen hombres y mujeres que producen desigualdades, generalmente hacia las mujeres. Los objetivos del presente estudio fueron conocer las propiedades psicométricas del Inventario de Sexismo Ambivalente en una muestra de estudiantes de Educación Física de Chile y estudiar las diferencias del sexismo según sexo y año cursado en la carrera. La muestra estuvo constituida por 265 estudiantes de Educación Física de dos Universidades de Chile. Del total, 106 fueron mujeres (40.0%). Los resultados muestran que el inventario posee niveles adecuados de validez y confiabilidad en la muestra estudiada. El sexismo benevolente y hostil presenta mayores puntajes en los hombres (p=.000) y en los cursos de primer y segundo año en comparación con cuarto y quinto (p=.000). Se concluye que el instrumento es válido y confiable para ser aplicado a este tipo de muestras y que los hombres que estudian educación física y los dos primeros años de la carrera presentan los mayores niveles de sexismo. Palabras claves: sexismo, educación física, universitarios. Abstract. Sexism corresponds to a set of beliefs of the characteristics that men and women possess that produce inequalities, generally towards women. The objectives of this study were to know the psychometric properties of the Inventory of Ambivalent Sexism in a sample of Physical Education students in Chile and to study the differences in sexism according to sex and year of study. The sample consisted of 265 physical education students from two Chilean universities. Of the total, 106 were women (40.0%). The results show that the inventory has adequate levels of validity and reliability in the sample studied. Benevolent and hostile sexism has higher scores in men (p=.000) and in first and second year courses compared to fourth and fifth (p=.000). It is concluded that the instrument is valid and reliable to be applied to this type of samples and that men who study physical education and the first two years of the career present the highest levels of sexism. Keywords: sexism, physical education, university

Published
2023

36. Assessing three industrially produced fungi for the bioremediation of diclofenac

Author

María Fernanda Blanco-Orta, Ramón Fernando García-de la Cruz, Luz María Teresita Paz-Maldonado, Diana Alejandra Pedraza-González, Mariana Montserrat Morales-Avila, Víctor Emmanuel Balderas-Hernández, Omar González-Ortega, and Ana Silvia Pérez-Martínez

Subjects
Environmental Engineering, General Medicine
Published
2023

37. Salivary gland ultrasound in clinical practice: What is its real usefulness?

Author

Karen Carpio Astudillo, Fernando Montero Reyes, Iustina Janta, Juan Molina Collada, Ana M. Anzola, Liz R. Caballero Motta, Belén Serrano-Benavente, Julia Martínez-Barrio, Alfonso Ariza Lapuente, Javier Rivera Redondo, Carlos González-Fernández, Indalecio Monteagudo, José María Álvaro-Gracia, and Juan Carlos Nieto-González

Subjects
Rheumatology, General Medicine
Published
2023

38. Connexin 43 expression in tooth germ and benign odontogenic tumors

Author

Felipe Martins Silveira, Nelly Molina-Frechero, Sandra López-Verdín, Natalia González Umpiérrez, Marcelo Gómez Palacio-Gastellum, Oscar Almeda-Ojeda, Rogelio González-González, Manoela Domingues Martins, Estefania Sicco, Vanesa Pereira-Prado, and Ronell Bologna-Molina

Subjects
Radiology, Nuclear Medicine and imaging, Dentistry (miscellaneous), Surgery, Oral Surgery, Pathology and Forensic Medicine
Published
2023

40. Resonancia magnética para portadores de dispositivos cardiovasculares. Consenso SEC-GT CRMTC/SEC-Asociación del Ritmo Cardiaco/SERAM/SEICAT

Author

Manuel Barreiro-Pérez, Beatriz Cabeza, David Calvo, José Luis Reyes-Juárez, Tomás Datino, Eliseo Vañó Galván, Alicia M. Maceira González, Carlos Delgado Sánchez-Gracián, Susanna Prat-González, Rosario J. Perea, Gorka Bastarrika, Marcelo Sánchez, Luis Jesús Jiménez-Borreguero, Covadonga Fernández-Golfín Lobán, José F. Rodríguez Palomares, José María Tolosana, José Alberto Hidalgo Pérez, Esther Pérez-David, Vicente Bertomeu-González, and Hug Cuéllar

Subjects
Radiology, Nuclear Medicine and imaging, Cardiology and Cardiovascular Medicine
Published
2023

42. Diagnosis on social impact measurement in the Matanzas science and technology park

Author

Yoandy Sergio Villalonga Arencibia, Lourdes Tarifa Lozano, María de Lourdes Artola Pimentel, Edith Adolfina González Palmira, Yudith Acosta González, and Leyda Finalé de la Cruz

Subjects
General Medicine
Abstract

The current Cuban context evidences a boom in research projects involving science, technology and innovation. Our National Innovation Management System is focused on the solution of the problems presented by our socialist social development economic model, with the use of knowledge and innovation. As part of the Informatization Policy of the country, the Scientific and Technological Parks (PCT) arise in Cuba, with which an accelerated development of R+D+i projects is propitiated in order to solve the fundamental problems of this model. At the express request of the country’s management, the projects generated by the PCTs in order to fulfill their mission and social transformation, it is essential to measure their social impact; however, the review of their design and a diagnosis (using methods such as surveys, interviews, documentary analysis) of the skills of the project managers working in the PCTCs, show marked weaknesses in measuring the social impact of their projects, which is the main objective of the present work: to show the results of the diagnosis carried out from the definition of indicators and measurement criteria for this purpose, as well as the authors’ analysis of the possible ways of solution.

Published
2023

44. Coronary Obstruction After Transcatheter Aortic Valve Replacement

Author

Soledad Ojeda, Rafael González-Manzanares, Pilar Jiménez-Quevedo, Pablo Piñón, Lluis Asmarats, Ignacio Amat-Santos, Eduard Fernández-Nofrerias, Raquel del Valle, Erika Muñoz-García, Maria-Cruz Ferrer-Gracia, Jose María de la Torre, Valeriano Ruiz-Quevedo, Ander Regueiro, Dario Sanmiguel, Sergio García-Blas, Jaime Elízaga, Jose Antonio Baz, Rafael Romaguera, Ignacio Cruz-González, José Moreu, Livia L. Gheorghe, Luisa Salido, Raúl Moreno, Cristóbal Urbano, Vicenc Serra, and Manuel Pan

Subjects
Cardiology and Cardiovascular Medicine
Published
2023

45. Valoración multidisciplinar de las secuelas al mes del alta hospitalaria por neumonía grave COVID-19, ¿existen diferencias en función de la terapia respiratoria empleada durante su ingreso en Cuidados Intensivos?

Author

A.M. Sánchez-García, P. Martínez-López, A.M. Gómez-González, J. Rodriguez-Capitán, R.J. Jiménez-López, J.M. García Almeida, E. Avanesi-Molina, N. Zamboschi, C. Rueda-Molina, V. Doncel-Abad, A.I. Molina-Ramos, E. Cabrera-César, I. Ben-Abdellatif, M. Gordillo-Resina, E. Pérez-Mesa, M. Nieto-González, P. Nuevo-Ortega, C. Reina-Artacho, P.L. Sánchez Fernández, M.F. Jiménez-Navarro, and M.A. Estecha-Foncea

Subjects
Critical Care and Intensive Care Medicine
Published
2023

46. Magnetic resonance in patients with cardiovascular devices. SEC-GT CRMTC/SEC-Heart Rhythm Association/SERAM/SEICAT consensus document

Author

Manuel Barreiro-Pérez, Beatriz Cabeza, David Calvo, José Luis Reyes-Juárez, Tomás Datino, Eliseo Vañó Galván, Alicia M. Maceira González, Carlos Delgado Sánchez-Gracián, Susanna Prat-González, Rosario J. Perea, Gorka Bastarrika, Marcelo Sánchez, Luis Jesús Jiménez-Borreguero, Covadonga Fernández-Golfín Lobán, José F. Rodríguez Palomares, José María Tolosana, José Alberto Hidalgo Pérez, Esther Pérez-David, Vicente Bertomeu-González, and Hug Cuéllar

Subjects
General Earth and Planetary Sciences, General Medicine, General Environmental Science
Abstract

Magnetic resonance has become a first-line imaging modality in various clinical scenarios. The number of patients with different cardiovascular devices, including cardiac implantable electronic devices, has increased exponentially. Although there have been reports of risks associated with exposure to magnetic resonance in these patients, the clinical evidence now supports the safety of performing these studies under specific conditions and following recommendations to minimize possible risks. This document was written by the Working Group on Cardiac Magnetic Resonance Imaging and Cardiac Computed Tomography of the Spanish Society of Cardiology (SEC-GT CRMTC), the Heart Rhythm Association of the Spanish Society of Cardiology (SEC-Heart Rhythm Association), the Spanish Society of Medical Radiology (SERAM), and the Spanish Society of Cardiothoracic Imaging (SEICAT). The document reviews the clinical evidence available in this field and establishes a series of recommendations so that patients with cardiovascular devices can safely access this diagnostic tool. Full English text available from: www.revespcardiol.org/en.

Published
2023

48. Invaginaciones intestinales en el adulto: localización, etiología, sintomatología y manejo terapéutico

Author

R. García Figueiras, S. Baleato-González, S. Sánchez Bernal, J.D. García Palacios, C. González-Carrero Sixto, M. Bravo González, and S. Junquera Olay

Subjects
Gynecology, medicine.medical_specialty, business.industry, Medicine, Radiology, Nuclear Medicine and imaging, Intestinal intussusception, business
Abstract

Resumen Objetivo Las invaginaciones intestinales en adultos son de dificil diagnostico debido a la inespecificidad de los sintomas. Sin embargo, la mayoria tienen una causa estructural que requiere tratamiento quirurgico. El objetivo de este estudio es revisar sus caracteristicas epidemiologicas, hallazgos en imagen y manejo terapeutico. Materiales y metodos Estudio retrospectivo de las invaginaciones intestinales que precisaron ingreso hospitalario diagnosticadas en nuestro hospital entre 2016 y 2020. De un total de 73 casos fueron excluidos errores de codificacion (n = 6) y pacientes menores de 16 anos (n = 46), resultando 21 invaginaciones en adultos. Resultados La edad media fue de 57 anos, y el dolor abdominal fue la manifestacion clinica mas frecuente en el 38% de los casos (n = 8). El diagnostico mediante tomografia computarizada (TC), con la presencia “del signo de la diana”, alcanzo una sensibilidad del 100%, siendo la region ileocecal la localizacion mas frecuente en un 38% de los pacientes (n = 8). Un 85,7% de los casos (n = 18) tenian una causa estructural y el 81% (n = 17) requirio cirugia. Los resultados anatomopatologicos fueron concordantes con la TC en un 94,1%, siendo la etiologia mas frecuente la neoplasica: 35,3% benignas (n = 6) y 64,7% malignas (n = 9). Conclusiones La TC es la prueba de eleccion en el diagnostico de las invaginaciones intestinales y resulta determinante a la hora de identificar la etiologia y decidir el manejo terapeutico.

Published
2023

50. Potencial turístico de la antigua hacienda el detalle de Mario Moreno Cantinflas en Ciudad Valles, San Luis Potosí

Author

Alma Rafaela Bojórquez Vargas, Herman Oscey González Domínguez, Carlos Pedraza Gómez, Gisela Beatriz Hernández González, and Romano Gino Segrado Pavón

Subjects
Conservación del Patrimonio, Patrimonio Edificado, Haciendas Turísticas, General Engineering, Vocación Turística, Potencial Turístico
Abstract

El objetivo consistió en analizar la preferencia de la demanda respecto a las estrategias potenciales de aprovechamiento de la antigua hacienda; así como, analizar las condiciones físico arquitectónicas de la misma. Metodología: se aplicaron cuestionarios semiestructurados, observación directa y entrevistas a profundidad a habitantes del ejido. Resultados: la opción de usarla como Hacienda turística fue la más demandada La hacienda presenta deterioro principalmente por intemperismo y el saqueo por abandono, pero no se pierde el interés por ser visitada.

Published
2023

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