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Radiation cystitis is a complication of radiation treatment given for pelvic malignancies. Radiation cystitis can present as mild symptoms of dysuria, frequency, and hematuria to severe life-threatening complications like intractable hematuria, bladder perforation and contracted non-functional urinary bladder, fistula etc. Treatment is tailored according to the severity of symptoms and available treatment modalities sequentially. Initial treatment of hematuria is intravenous fluids, blood transfusions, cystoscopy and fulguration, irrigation with intravesical agents like alum, hyperbaric oxygen therapy, angioembolisation, urinary diversion and surgery (cystectomy). Radiation cystitis is associated with short- and long-term complications in pelvic malignancies patients. Early diagnosis, follow up, treatment is crucial for decreasing morbidity and mortality in these patients. In this review article we describe the current protocol in diagnosing and managing radiation cystitis. Keywords Pelvis, Radiation, Hematuria, intravesical treatments, Alum, formalin

Wireless body area networks (WBANs) have seen an increase in popularity in recent years. Electromagnetic waves created by the body have the capacity to connect nodes all over the epidermis and throughout the body. If the gadget does not cause discomfort or harm, it can be linked to or implanted in the body. This is something that is currently being worked on. Other factors influence an individual’s genuine mobility and the ease with which they can use something. Participating in social networks may enhance the lives of members. WBANs equipped with sensors can monitor a user’s heart rate and communicate that information to the user’s physician. WBAN has been shown to be a dependable electronic health solution. WBAN technology allows you to follow your patient’s data no matter where they are, when they are, or what they are doing. However, because it runs in an open Wi-Fi environment and can conceal users’ physiological data, it is more vulnerable to assault. To deal with resource-constrained WBAN sensors and devices, a cryptographic solution that is both very efficient and extremely secure is required. Our primary priority will be the safeguarding of the WBAN network. WBAN contains several significant security weaknesses that must be addressed immediately. WBANs might benefit from certificateless signature encryption that uses a hyperelliptic curve and works over a secure channel. We are outpaced by the opposition by 4.58 milliseconds.

Mullerianosis of the urinary bladder is a rare bladder lesion with fewer than 30 cases reported in the literature. It describes the ectopic presence of endocervical, endometrium, and endosalpingial tissues inside the urinary bladder. It is diagnosed when at least two of three Mullerian tissues, endometriosis, endocervicosis, and endosalpingiosis, are identified. Mullerianosis presents in females of the reproductive age group, especially after pelvic surgery. Treatment involves resection of the bladder mass and ruling out malignant pathology. Follow-up of Mullerianosis is vital, as recurrence is common. Medical therapy is also indicated when a diagnosis is established after the histopathological examination of the tumor.We report a 31-year-old female presenting with recurrent lower abdominal pain. Cystoscopy showed a mass of 3 × 3 cm in the urinary bladder. Transurethral resection of bladder tumor was resected, and histopathology suggested a combination of endometriosis, endocervicosis, and endosalpingiosis. Pain was resolved after surgery, but the lesion recurred at 3 months, which was subsequently resected.

Data analysis involves the deployment of sophisticated approaches from data mining methods, information theory, and artificial intelligence in various fields like tourism, hospitality, and so on for the extraction of knowledge from the gathered and preprocessed data. In tourism, pattern analysis or data analysis using classification is significant for finding the patterns that represent new and potentially useful information or knowledge about the destination and other data. Several data mining techniques are introduced for the classification of data or patterns. However, overfitting, less accuracy, local minima, sensitive to noise are the drawbacks in some existing data mining classification methods. To overcome these challenges, Support vector machine with Red deer optimization (SVM-RDO) based data mining strategy is proposed in this article. Extended Kalman filter (EKF) is utilized in the first phase, i.e., data cleaning to remove the noise and missing values from the input data. Mantaray foraging algorithm (MaFA) is used in the data selection phase, in which the significant data are selected for the further process to reduce the computational complexity. The final phase is the classification, in which SVM-RDO is proposed to access the useful pattern from the selected data. PYTHON is the implementation tool used for the experiment of the proposed model. The experimental analysis is done to show the efficacy of the proposed work. From the experimental results, the proposed SVM-RDO achieved better accuracy, precision, recall, and F1 score than the existing methods for the tourism dataset. Thus, it is showed the effectiveness of the proposed SVM-RDO for pattern analysis.

The unremitting COVID-19 pandemic is evoking anxiety, fear, panic, and socio-occupational stress among human population all around the globe. This cross-sectional study was planned to assess the fear and anxiety because of COVID-19 pandemic among health care professionals, patients, and general public in IGMC, Shimla. A cross-sectional, web-based survey was conducted in month of December 2020. COVID-19 fear questionnaire was used to assess fear among participants. A total of 162 responses were received. Ninety-five (41.35%) respondents were males and 67(58.64%) were females. Average total score of COVID-19 fear based on questionnaire was 17.71 among the participants. The majority of respondents 67(41.4%) were afraid of COVID-19, 53(32.7%) were neutral, whereas 42 (25.9%) were not afraid of COVID-19. Sixty-two (38.3%) responders were uncomfortable thinking about COVID-19, whereas, 67(41.4%) were not uncomfortable. Only 22 (13.6%) participants felt their hands clammy when they thought of COVID-19, 78 (48.15%) respondents were afraid of losing their life, 78 (48.15%) respondents disagreed with the question of being nervous or anxious to hear news and stories of COVID-19. Thirteen (8%) participants were informed of sleeplessness, and 22 (13.5%) reported palpitations due to COVID-19 fear. COVID-19 fear was evident in 65.15% of all males and 55.8% of all females’ respondents, respectively. Seventy percent of participants of age more than 40 years were afraid of COVID-19, whereas only 54.83% participants of less than 40 years had COVID-19 fear. COVID-19 fear was more evident among non-HCW (74.42%) as compared to HCW in whom it was seen in 53.03% participants. Rampaging Pandemic has ingrained fear and anxiety among non-HCW as well as health care workers. But undeterred by COVID-19 fear, majority of participants didn’t fear death and didn’t have other effects of fear. Despite (can be used) surging pandemic when fear of COVID-19 is soaring, HCW and non-HCW could work, think, and live.

Abortions in dairy animals can be caused by several infectious agents. Identification of the actual causal agent(s) is important for formulating suitable control strategies. A 3-year (2016–2018) longitudinal study was conducted in a dairy farm following an abortion storm in the mid- to late gestations. The investigation focused on the seven major infectious abortifacient in cattle, viz. bovine alphaherpesvirus-1 (BoHV-1), bovine viral diarrhoea virus (BVDV), Neospora caninum, Brucella abortus, Coxiella burnetii, Leptospira Hardjo, and Listeria monocytogenes. High seroprevalence was observed for BVDV (79.4%), Leptospira (70.5%), BoHV-1 (53.5%), and Brucella (45.0%) at the beginning of the investigation (August 2016). The incidence proportion increased for BVDV, Leptospira, and Brucella in the following years of the investigation. A strong association of Brucella seropositivity with history of abortion (OR = 3.27) was recorded. Incidence of BoHV-1 reduced during the period of study coincident with systematic IBR inactivated marker vaccination of the herd. Sixty-four abortion cases were investigated for the identification of causative agent(s) by microbial culture, serological (ELISA), and molecular detection (PCR/ real-time PCR). Antibodies to BVDV, Brucella, BoHV-1, Leptospira, Neospora, and Coxiella were detected in 63, 61, 56, 35, 5, and 6 aborting cattle, respectively. Real-time PCR/PCR of clinical specimens detected DNA of Brucella, BoHV-1, Coxiella, Leptospira, and Listeria in 34, 13, 12, 9, and 4 abortion cases, respectively. BVDV and Neospora were not detected in any specimen samples. Brucella abortus isolated from the farm was determined as ST1 by multi-locus sequence typing (MLST). DNA of multiple agents were detected in 21 of the 64 cases (43.75%). Overall, the data suggests, Brucella was the major causative agent, although multiple causative agents circulated in the farm. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-020-00414-x.

India is the largest milk producer in the world with 22 percent of the global share and engages around 6.3 crore rural households. The cost and availability of animal health services for small dairy holders in a country like India has always been a cause of concern. There is a growing apprehension about the presence of drug residues in milk and milk products as well emergence of drug resistant bacteria and its associated health risks due to indiscriminate use of these products. The cost of therapeutic medicines, antimicrobial resistance and the residues in milk, meat and other animal products have forced the animal health sector to look for alternate forms of therapy to overcome these deficiencies. The new alternatives should be affordable, farmer friendly and lead to minimal or no residues in milk and other products. Practice of Ethno-Veterinary Medicine (EVM) is an approach that fulfils the above criteria. However, animal health service providers have very limited knowledge about EVM which in India is largely based on Veterinary Ayurveda. EVM has been used throughout the world by traditional animal rearing communities. EVM has been used for most common ailments in almost all domestic animal species. While there are efforts to document these practices, such documents are not widely available. Only recently, scientific scrutiny of these treatments for efficacy, mode of action and active ingredients has been initiated. National Dairy Development Board (NDDB) along with Sabarkantha District Cooperative Milk Producers Union, Sabarkantha, Gujarat and Trans-Disciplinary University, Bengaluru, evaluated the use of EVM initially for management of bovine mastitis and subsequently for management of other common ailments of dairy animals. It was observed that EVM was helpful in successfully managing mastitis (85%), diarrhoea (86%), fever (85%), indigestion (87%), anoestrus (77%), worm infestation (89%), wound healing (79%) and ecto-parasite infestation (87%) with high clinical cure rate. It was economical compared to conventional allopathic treatment. A reduction in residues of common antibiotics (quinolones, tetracycline, sulphonamide, s-lactam) in milk was observed in the area of operation. Drug resistant bacteria ( S. aureus including methicillin resistant S. aureus – MRSA; and E. coli including Extended Spectrum Beta Lactamase producing – E. coli - ESBL), were isolated from the milk of bovines suffering from mastitis. EVM was able to cure even these animals. However, awareness about EVM is limited among animal health service providers and other stakeholders. EVM needs to be given adequate focus and mainstreamed. This would entail concerted efforts and initiatives in policy, research and development and infrastructure.

Seven ELISA kits were evaluated for the fitness of purpose in diagnosing brucellosis among cattle and buffaloes in the endemic scenarios of India. The sera (675 numbers) for the study were sourced from brucellosis-free as well as infected herds. The diagnostic sensitivity (dsn) and specificity (dsp) of the kits were determined by three approaches: based on the results of the Rose Bengal test, history of the animals (sera from infected or naïve animals), and based on the results obtained from the 'majority of the tests'. The dsn and dsp ranged from 65.10% to 98.66%, and 98.04% to 100% respectively. The results and suitability of the kits for diagnostic application in various epidemiological situations were discussed.

Priapism is an uncommon urological emergency. Drugs are known causes of priapism. Self-medication and use of over-the-counter drugs are very common in India. Withania somnifera is common over the counter herb used in India for anxiety, depression, boosting immunity, infertility, etc. Despite the unprohibited and over-the-counter use of drug, the efficacy and adverse events data are not available. We report a patient of anxiety and depression self-medicating with W. somnifera and multivitamins presenting with ischemic priapism.

Bovine tropical theileriosis, a tick-borne disease, causes huge economic loss to the Indian dairy industry. Theileriosis in India is mainly caused by Theileria annulata, although the presence of T. orientalis has also been reported. The present study was undertaken to investigate the deaths of cross-bred Holstein Friesen (CBHF) cows on a farm in the state of Telangana, India. Deceased animals had recently calved and prior to death had developed high fever (107 °F) and anaemia. Infected cows were infested with ticks (Hyalomma species). Theileria piroplasms were noticed in the Giemsa stained blood smears. PCR assays further confirmed the presence of Theileria in the blood samples of the infected cows. Partial Tams1 gene sequences from the infected animals shared 99.87% to 100% identity scores with the sequences of Sri Lankan isolates recently proposed as a novel Theileria species (provisionally designated as Theileria sp. Yokoyama). To the best of our knowledge, this is the first report of the novel species of Theileria from India. Infected animals were effectively treated with buparvaquone and oxytetracycline. The introduction of new animals into the farm without risk assessment was found to be a major cause of the outbreak.

Bovine anaplasmosis is one of the most important tick borne disease in ruminants causing huge economic loss to the dairy industry. A cross-sectional study was carried out to detect serum antibodies to Anaplasma infection in cattle and buffaloes housed in 14 organized herds located at various climatic zones spreading over 9 different states in India. A total of 911 serum samples, collected from 667 cattle and 244 buffaloes, were subjected to a competitive enzyme linked immune-sorbent assay detecting an epitope of major surface protein 5 (MSP5) of Anaplasma. The overall true prevalence was 48.72% (95% CI 45.13–52.32%). The prevalence rate was higher in cattle (51.58%) than buffaloes (40.89%) and the difference was statistically significant (p

This paper presents different Data Mining Algorithms to be used to access data patterns in Tourism Industry and their applications using Data Mining Tool like WEKA which is a popular software, and useful patterns can be found efficiently. Data Mining, as the name implies is the science by which hidden and interesting information can be found from large datasets analogous to Gold Miners who extract precious metal sieving through a lot of raw material. Normal day to day business operations create huge voluminous data which is needed to be utilized properly to find information to make efficient, effective, and prompt decisions otherwise businesses can not sustain in competitive environment. There are some techniques and algorithms used in data mining for determining interesting hidden patterns. data mining algorithms are becoming popular day by day in various applications like Travel and Tourism. Nowadays, a number of data mining applications are available for Public use. Therefore, we have to judge which particular algorithm or application suits a problem domain. The idea behind applying data mining is very straight forward, it is similar to a human being becoming intelligent from learning by examples and experience. Similarly, in data mining the behavior of given system (training dataset) is learned by application, and certain rules are developed. Then these rules are used to evaluate the behavior/result for the given situation (test dataset). The overall goal of this paper is to classify some data mining algorithms and applications, draw a comparative study that which one is better for tourism industry and under what circumstances.

Bovine anaplasmosis is one of the most important tick borne disease in ruminants causing huge economic loss to the dairy industry. A cross-sectional study was carried out to detect serum antibodies to Anaplasma infection in cattle and buffaloes housed in 14 organized herds located at various climatic zones spreading over 9 different states in India. A total of 911 serum samples, collected from 667 cattle and 244 buffaloes, were subjected to a competitive enzyme linked immune-sorbent assay detecting an epitope of major surface protein 5 (MSP5) of Anaplasma. The overall true prevalence was 48.72% (95% CI 45.13–52.32%). The prevalence rate was higher in cattle (51.58%) than buffaloes (40.89%) and the difference was statistically significant (p

Infectious bovine rhinotracheitis (IBR) caused by bovine alphaherpesvirus 1 (BoHV-1) is an economically important disease of cattle and buffaloes. Following acute infection, the virus usually attains latency in the sensory neurons. Stress-induced reactivation of latency can cause the infected animals to intermittently shed the virus in body secretions including semen. A longitudinal analysis was carried out to study BoHV-1 shedding in the semen of IBR seropositive cattle and buffaloes. The study involved data generated from the screening of 119,850 extended frozen semen (EFS) batches, collected from 1,229 IBR seropositive bulls, over a period of four years (April 2015 to March 2019). A TaqMan based real-time PCR assay was employed to detect the gB gene BoHV-1 DNA in the EFS batch samples. Each sample was tested in duplicate and amplification in any of the replicates at or below the threshold cycle (Ct ≤ 40) was considered positive. The overall positivity of BoHV-1 in EFS batches was 1.18%. About 41% of the bulls (509 of 1,229) were found to have excreted the virus in semen at least once during the study period. The frequency of viral shedding in buffaloes (0.96%) was significantly lower than that of cattle (1.3%) (p 0.001). No significant difference was noted in the rate of shedding between the first and the second ejaculates collected on the same day (p = 0.607). The rate of shedding also did not vary among various breeds of cattle (p = 0.454) or with the age of the bulls (p = 0.054). No significant variation in the shedding rate was observed in cattle across different seasons (p = 0.101); while in buffaloes, the rate was higher in autumn (1.2%) than in winter (0.7%) (p = 0.037). The difference in positivity among semen stations was statistically significant (p 0.001). Analysis of data revealed that ≥100 EFS batch samples/bull were screened from 361 of the 1,229 bulls included in the study. None of the EFS batches screened from 39 of these 361 bulls were found positive during the four years, suggesting they were non-shedders. Further research is warranted to delineate the underlying features of the seropositive non-shedders; following which an adequate risk assessment may be made for the maintenance of infected but non-shedding bulls in semen production.

Made available in DSpace on 2020-12-10T17:04:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Charles T. McCord Jr Chair in Petroleum Geology at Louisiana State University Ministry of Earth Sciences, Government of India National Centre for Polar and Ocean Research (NCPOR) National Research Foundation of Korea CSIR-National Institute of Oceanography, India A giant mass-transport complex was recently discovered in the eastern Arabian Sea, exceeding in volume all but one other known complex on passive margins worldwide. The complex, named the Nataraja Slide, was drilled by International Ocean Discovery Program (IODP) Expedition 355 in two locations where it is similar to 300 m (Site U1456) and similar to 200 m thick (Site U1457). The top of this mass-transport complex is defined by the presence of both reworked microfossil assemblages and deformation structures, such as folding and faulting. The deposit consists of two main phases of mass wasting, each consisting of smaller pulses, with generally fining-upward cycles, all emplaced just prior to 10.8 Ma based on biostratigraphy. The base of the deposit at each site is composed largely of matrix-supported carbonate breccia that is interpreted as the product of debris-flows. In the first phase, these breccias alternate with well-sorted calcar-enites deposited from a high-energy current, coherent limestone blocks that are derived directly from the Indian continental margin, and a few clastic mudstone beds. In the second phase, at the top of the deposit, muddy turbidites dominate and become increasingly more siliciclastic. At Site U1456, where both phases are seen, a 20-m section of hemipelagic mudstone is present, overlain by a similar to 40-m-thick section of calcarenite and slumped interbedded mud and siltstone. Bulk sediment geochemistry, heavy-mineral analysis, clay mineralogy, isotope geochemistry, and detrital zircon U-Pb ages constrain the provenance of the clastic, muddy material to being reworked, Indus-derived sediment, with input from western Indian rivers (e.g., Narmada and Tapti rivers), and some material from the Deccan Traps. The carbonate blocks found within the breccias are shallow-water limestones from the outer western Indian continental shelf, which was oversteepened from enhanced clastic sediment delivery during the mid-Miocene. The final emplacement of the material was likely related to seismicity as there are modern intraplate earthquakes close to the source of the slide. Although we hypothesize that this area is at low risk for future mass wasting events, it should be noted that other oversteepened continental margins around the world could be at risk for mass failure as large as the Nataraja Slide. Louisiana State Univ, Dept Geol & Geophys, E253 Howe Russell Kniffen Geosci Complex, Baton Rouge, LA 70803 USA Texas A&M Univ, Int Ocean Discovery Program, 1000 Discovery Dr, College Stn, TX 77845 USA Woods Hole Oceanog Inst, Dept Geol & Geophys, Woods Hole, MA 02543 USA UCL, Dept Earth Sci, Gower St, London WC1E 6BT, England Univ Toulouse 3 Paul Sabatier, Geosci Environm Toulouse, 14 Ave Edouard Belin, F-31400 Toulouse, France GeoSep Serv, 1521 Pine Cone Rd, Moscow, ID 83843 USA Univ Queensland, Sch Earth & Environm Sci, St Lucia, Qld 4072, Australia Natl Ctr Polar & Ocean Res, Vasco Da Gama 403804, Goa, India Univ Milano Bicocca, Dept Earth & Environm Sci, Piazza Sci 4, I-20126 Milan, Italy Purdue Univ, Dept Earth Atmospher & Planetary Sci, 550 Stadium Mall Dr, W Lafayette, IN 47907 USA Univ Birmingham, Sch Geog Earth & Environm Sci, Birmingham B15 2TT, W Midlands, England Macquarie Univ, Fac Sci & Engn, Dept Earth & Planetary Sci, N Ryde, NSW 2109, Australia Ohio State Univ, Sch Earth Sci, 275 Mendenhall Lab,125 South Oval Mall, Columbus, OH 43210 USA Birbal Sahni Inst Palaeosci, 53 Univ Rd, Lucknow 226007, Uttar Pradesh, India Univ Bremen, MARUM, Leobener Str, D-28359 Bremen, Germany Kochi Univ, Dept Nat Environm Sci, 2-5-1 Akebono Cho, Kochi 7808520, Japan Pusan Natl Univ, Div Earth Environm Syst, Busan 60973, South Korea Wadia Inst Himalayan Geol, 33 GMS Rd, Dehra Dun 248001, Uttrakhand, India Natl Inst Ocean Technol, Marine Biotechnol Dept, Velacheiy Tambaram Main Rd, Chennai 600100, Tamil Nadu, India Columbia Univ, Ctr Climate Syst Res, 2880 Broadway, New York, NY 10025 USA Nanjing Univ, Sch Geog & Oceanog Sci, 163 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China Oregon State Univ, Coll Earth Ocean & Atmospher Sci, 104 CEOAS Adm Bldg, Corvallis, OR 97331 USA Natl Ctr Earth Sci Studies, Geosci Div, Aakkulam Trivandrum 695031, India Natl Inst Oceanog, Geol Oceanog Div, Panaji 403004, Goa, India Oil & Nat Gas Commiss, 11 High,Bandra Sion Link Rd, Mumbai 400017, Maharashtra, India Univ Estadual Paulista, Inst Geociencias & Ciencias Exatas, 1515 Ave 24-A, BR-13506900 Rio Claro, SP, Brazil Kumaun Univ, Dept Geol, Naini Tal 263002, India Banaras Hindu Univ, Dept Geol, Varanasi 221005, Uttar Pradesh, India Xiamen Univ, Dept Geol Oceanog, Xiamen 361102, Fujian, Peoples R China Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China Hokkaido Univ, Grad Sch Environm Sci, Kita Ku, N10W5, Sapporo, Hokkaido 0600810, Japan Scripps Inst Oceanog, 9500 Gilman Dr, La Jolla, CA 92093 USA Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China Univ Estadual Paulista, Inst Geociencias & Ciencias Exatas, 1515 Ave 24-A, BR-13506900 Rio Claro, SP, Brazil National Centre for Polar and Ocean Research (NCPOR): 90/2018 National Research Foundation of Korea: 2016R1A2B4008256 National Research Foundation of Korea: 2019R1A2C1007701

Different temperature zones have significant impact on the population dynamics of Plutella xylostella. Effective management of P. xylostella requires the knowledge of temperature tolerance by different life stages. In the current study, fitness parameters of diamondback moth were reported by using age-stage, two-sex life table traits at four constant temperatures (15, 20, 25 and 30 °C). The life cycle of P. xylostella was significantly longer at 15 °C. The 20 °C level of temperature was found optimal for fecundity, gross reproductive rate (51.74 offspring) and net reproductive rate (44.35 offspring per individual). The adult pre-oviposition period was statistically at par at all four level of temperatures. However, the survival was maximum at 20 °C as compared to other three temperature ranges. Based on the current study, it was concluded that temperature has a great role in population build-up of P. xylostella and effective management tactics should be applied to prevent significant damage to cabbage and other cruciferous crops when the temperature in the field is near 20 °C. Keywords: Age-stage, Plutella xylostella, Lepidoptera, Temperature, Life table

Highlights • Retrieval of BoHV-1 DNA from extended semen spotted paper matrix was optimized. • The method was repeatable in inter-assay and intra-assay precision testing. • Viral DNA was stable in the matrix for at least 28 days when stored at 4°–37 °C. • The analytical sensitivity was equivalent to the OIE approved Chelex based method. • The dsn and dsp was 83% and 93% respectively in comparison to Chelex based method., The extended frozen semen (EFS) batches produced from infectious bovine rhinotracheitis (IBR) sero-positive cattle and buffalo bulls housed in various semen stations in India are transported to the testing laboratory in liquid nitrogen (LN2) for screening bovine herpesvirus-1 (BoHV-1). This procedure is laborious and poses LN2 related hazards. An alternative logistics for transportation of samples was investigated. Use of Flinders Technology Associates (FTA®) elute card was evaluated for transportation of extended bovine semen to screen BoHV-1 DNA by real-time PCR targeting gB gene and the method was compared with the OIE approved Chelex resin based method. A protocol for extraction of BoHV-1 DNA from FTA® card spotted with extended semen was optimized. The viral DNA was found to be stable on FTA® card for at least 28 days when the cards are stored at 4°–37 °C. The analytical sensitivity for the assay was determined using variable dilutions of BoHV-1 spiked semen and positive plasmid harbouring gB gene (97bp) spotted onto FTA® card and it was found to be 100.8 TCID50/ml or 100 copies respectively in real-time PCR. The test could detect as low as 100.008 TCID50/ml or 1 copy of positive plasmid when more number of replicates (n = 6) of the same sample were tested. This sensitivity was found to be comparable to Chelex method and both the methods demonstrated a very strong correlation (r = 0.9774; 95% CI: 0.9620–0.9860) in terms of Ct value (p

Bovine alphaherpesvirus-1 (BoHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is an economically important viral pathogen affecting cattle and buffaloes. Serological assays are mostly used for detection of the antibodies, but variation has been detected in the diagnostic performances of the individual assay. In the present study, four commercially available ELISA kits {two indirect ELISA (kits A and B) and two blocking ELISA (kits C and D)} were evaluated for the detection of antibodies against BoHV-1 in Indian cattle and buffaloes (fitness of purpose). The diagnostic sensitivity (dsn) and specificity (dsp) of these kits were determined by three ways; considering virus neutralization test (VNT) as gold standard test, using pre-test information of the samples, and majority of tests. Screening of 200 known negative sera (124 cattle, 76 buffaloes) sourced from IBR free farms revealed gB based ELISA kits are more specific than the indirect ELISA kits. Testing of 125 known positive sera (81 cattle, 44 buffaloes) suggests kit B be most sensitive followed by kit C, A and D. Interestingly, kit D was found to be most sensitive for detection of vaccination-induced BoHV-1 antibodies followed by kit B. Similar trend were also observed in the limit of dilution experiment performed using known infected and vaccinated sera. VNT was found to be the most specific test and its use as the gold standard test revealed all kits to have more than 99 % sensitivity. All the ELISA kits could detect BoHV-1 specific antibodies in the IBR vaccinated calves as early as 11 days post-vaccination. In Kappa statistics, an almost perfect agreement between the ELISA kits was recorded. The overall performance of the kits in serodiagnosis of IBR as determined by the area under curve in ROC analysis was good.

A duplex real‑time PCR was developed and validated for the simultaneous detection of Brucella and bovine alphaherpesvirus‑1 (BoHV‑1) from bovine clinical specimens. The bcsp31 gene of Brucella and gB gene of BoHV‑1 were used as targets in the assay. The limit of detection for BoHV‑1 was 0.03 TCID50 of virus and 10 plasmid copies containing the target gene while for Brucella it was 4.1 × 101 CFUs. Intra‑assay and inter‑assay values showed high repeatability and reproducibility of the assay. The diagnostic sensitivity (dsn) and diagnostic specificity (dsp) of the duplex assay were determined by screening 443 clinical specimens and comparing the results with the respective individual assays. The dsn and dsp for detection of Brucella were found to be 95.24% and 95.65%, respectively whereas for BoHV‑1, the dsn (100%) and dsp (99.47%) were slightly higher. The duplex assay had a very good degree of agreement with the respective individual real‑time PCR test {kappa value 0.97 for Brucella and 0.95 for BoHV‑1}. The results of the current study suggest that the duplex assay would be a cost‑effective and time‑saving alternative for the individual real‑time PCR assay for the detection of Brucella and BoHV‑1.

Tuberculosis (TB) in domestic and wild ruminants is mostly caused by Mycobacterium bovis (MB). However, the present paper describes the first report of TB of antelopes (Antelope cervicapra) and chinkara (Gazella bennettii) due to Mycobacterium tuberculosis (MT) in India. These wild hosts may represent a vehicle for the dissemination of MT infection to domestic livestock or human. MT was isolated by culture employing the MGITTM BACTECTM 960 (Becton Dickinson, BD) system and Middlebrook 7H10 Agar enriched with oleic albumin dextrose catalase (OADC) and Lowenstein-Jensen (LJ) medium supplemented with glycerol, but not sodium pyruvate. The isolates were confirmed as a member of the Mycobacterium tuberculosis complex (MTC) by using a commercial nested PCR that targeted the IS6110 sequence. Further confirmation of the isolates as MT strains was achieved by employing commercial line probe assay genotyping kits (Hain Lifescience, Germany) that specifically identifies MT within the MTC group.

Made available in DSpace on 2022-04-28T19:05:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-21 In the Arabian Sea, South Asian monsoon (SAM)-induced high surface water productivity coupled with poor ventilation of intermediate water results in strong denitrification within the oxygen minimum zone (OMZ). Despite the significance of denitrification in the Arabian Sea, we have no long-term record of its evolution spanning the past several million years. Here, we present the first record of denitrification evolution since Late Miocene (∼10.2 Ma) in the Eastern Arabian Sea, where the SAM generates moderate surface water productivity, based on the samples retrieved during the International Ocean Discovery Program (IODP) Expedition 355. We find that (i) the SAM was persistently weaker from ∼10.2 to 3.1 Ma; it did not intensify at ∼8 Ma in contrast to a few previous studies, (ii) on tectonic timescale, both the SAM and the East Asian Monsoon (EAM) varied synchronously, (iii) the first evidence of denitrification and productivity/SAM intensification was at ∼3.2-2.8 Ma that coincided with Mid-Pliocene Warm Period (MPWP), and (iv) the modern strength of the OMZ where denitrification is a permanent feature was attained at ∼1.0 Ma. National Centre for Antarctic and Ocean Research Department of Oceanography Pusan National University Department of Geology and Geophysics Louisiana State University, E253 Howe-Russell-Kniffen, Geoscience Complex International Ocean Discovery Program Texas A&M University, 1000 Discovery Drive College Department of Earth and Environmental Sciences University of Milano Bicocca, Piazza della Scienza 4 School of Geography Earth and Environmental Sciences University of Birmingham, Edgbaston Department of Earth and Planetary Sciences Faculty of Science and Engineering Macquarie University Level 2, The Australian Hearing Hub, 16 University Avenue Department of Earth and Environmental Sciences University of Texas at Arlington, Geosciences Building, 500 Yates Street Manipal Centre for Natural Sciences Manipal University, Dr. T.M.A. Pai Planetarium Building MARUM University of Bremen, Leobener Strasse Center for Advanced Marine Core Research/Natural Science Cluster Kochi University, 2-5-1 Akebono-cho Wadia Institute of Himalayan Geology, 33 GMS Road Marine Biotechnology Department National Institute of Ocean Technology, Velachery-Tambaram Main Road Department of Earth Sciences University of Southern California, 3651 Trousdale Parkway School of Geographical and Oceanographical Sciences Nanjing University, 163 Xianlin Avenue College of Earth Ocean and Atmospheric Sciences Oregon State University, 104 CEOAS Administration Building Geosciences Division National Centre for Earth Science Studies University College London, Gower Street Geological Oceanography Division National Institute of Oceanography ONGC 11 High, Bandra-Sion Link Road Instituto de Geociências e Ciências Exatas Universidade Estadual Paulista, 1515 Avenida 24-A Department of Geology Kumaun University Department of Geology Banaras Hindu University Department of Geological Oceanography College of Ocean and Earth Sciences Xiamen University, Xiping Building, Xiang'an South Road Graduate School of Environmental Science Hokkaido University, N10W5, Kita-ku Scripps Institution of Oceanography, 9500 Gilman Drive Key Laboratory of Marine Geology and Environment Institute of Oceanology Chinese Academy of Sciences, 7 Nanhai Road Laboratoire Géosciences Paris-Sud (GEOPS UMR8148-CNRS) Université de Paris-Sud (Orsay), Bâtiment 504 Instituto de Geociências e Ciências Exatas Universidade Estadual Paulista, 1515 Avenida 24-A

This paper presents a record of radiolarian species from the sections (1H1-1H2) Leg 119 Site 738B of Southern Ocean region. Well diversified taxa of typical Antarctic assemblage are present in the sections. Thirty species were identified and illustrated from twenty-nine samples. On the basis of appearance, disappearance and abundance of taxa, viz. Omega and Psi zones are established and systematics of stratigraphically important species is described. These zones are equivalent to NR1 and NR2 of Upper Quaternary.

Background Traumatic amputation of the penis is a rare surgical emergency. Although repair techniques have been well described in literature, failure of replantation and its causes are poorly understood and reported. Herein, we report the case of a 9 year old boy who underwent replantation of his amputated penis with delayed failure of the surgery, along with a discussion of recent advances in the management of this condition. Case Presentation A 9-year-old boy was referred to our hospital for traumatic amputation of the penis. Papaverine aided microsurgical replantation of the severed part was performed, but by 48 h, the glans became discoloured and necrosis set in by 4 days. Unfortunately, by day 12 two thirds of the re-implanted penis was lost along with overlying skin. Conclusion Replantation of an amputated penis in a pediatric patient is a daunting task even for experienced surgeons. The vasodilatory effect of papaverine for vascular anastomosis is well described, but the use of a paediatric cannula for identification and instillation of papaverine into penile vasculature, has not been described for the repair of penile amputation. Despite its apparent failure, we believe this technique may be valuable to surgeons who might encounter this rare event in their surgical practice, especially in resource limited settings like ours.

The ODP Site 738B Leg 119 is located on the base of southern slope of Kerguelen Plateau. Twenty nine samples from two sections viz., 1H1-1H2 were used to carry out the present study. The polycystine radiolaria Cycladophora davisiana Ehrenberg first appears in upper Pliocene sediments in North Pacific at approximately 2.7 Ma. Peaks and curves of C. davisiana abundance are used as stratigraphic and Paleoceanographic markers indicating glacial period during the Quaternary and over the last 425 Kyrs. The two zones were established viz. Omega and Psi and which are equivalent to NR1 and NR2 of upper Quaternary. On the basis of abundances, C. davisiana having one peak in omega while eight in the Psi zone. Further, it is interpreted that in the Psi zone more cooling or glacial periods were present compared to Omega.

This paper presents a detailed record of radiolarian species from the Leg 119 Site 745 (sections 1H to 4H) in the southern ocean region. Forty five species were identified and illustrated from forty six samples. The taxonomy, two radiolarian zones namely NR1 and NR2, morphological variations and distribution of taxa have been made from these sections. Twelve new species are identified but they are not formally named. The study provides the detailed Antarctic Pleistocene radiolarian data, proposed the placement of NR1 and NR2 zonal boundary at a shallow depth of 6.63 mbsf and data is used for comparative studies with other parts of the Antarctic region.

This paper presents first comprehensive account of radiolarian taxa from the Pleistocene sequence of the Antarctic region. On the basis of 54 samples from the piston core KH94-4-AMR-2PC, 71 well-preserved radiolarian species were identified and illustrated. Their systematics, morphological variability and distribution in this Southern Ocean region are given and also compared with similar data available from other regions. Ten new taxa are described in detail but are yet to be formally named. This study provides a more extensive database and new taxa for comparative studies of other parts of the ocean sectors of the Antarctic region.

The Indian (southwest) summer monsoon is one of the most intense climatic phenomena on Earth. Its long-term development has been linked to the growth of high topography in South and Central Asia. The Indian continental margin, adjoining the Arabian Sea, offers a unique opportunity to investigate tectonic–climatic interactions and the net impact of these processes on weathering and erosion of the western Himalaya. During International Ocean Discovery Program Expedition 355, two sites (U1456 and U1457) were drilled in Laxmi Basin in the eastern Arabian Sea to document the coevolution of mountain building, weathering, erosion, and climate over a range of timescales. In addition, recovering basement from the eastern Arabian Sea provides constraints on the early rifting history of the western continental margin of India with special emphasis on continental breakup between India and the Seychelles and its relationship to the plume-related volcanism of the Deccan Plateau. Drilling and coring operations during Expedition 355 recovered sediment from Sites U1456 and U1457 in the Laxmi Basin, penetrating 1109.4 and 1108.6 m below seafloor (mbsf), respectively. Drilling reached sediment dated to 13.5–17.7 Ma (late early to early middle Miocene) at Site U1456, although with a large hiatus between the lowermost sediment and overlying deposits dated to