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Background: Considering the role of calcium in the replication and morphogenesis of rotaviruses, it is hypothesized that decreased cytosolic calcium levels by using calcium channel blockers can subsequently interfere with rotavirus replication. Objective: The present study investigated the effects of two calcium ion channel blockers, amlodipine and diltiazem, against human rotavirus infection. Method: Cytotoxic effects of the drugs on MA-104 cells were evaluated using the neutral red assay. The effects of amlodipine and diltiazem at non-toxic concentrations on human rotavirus were examined using cytopathic effect inhibition, TCID50, and real-time PCR assays. Results: The highest inhibitory effect was obtained at concentrations of 0.5 μg/ml of amlodipine and 3 μg/ml of diltiazem, leading to 4.6 and 5.5 logarithmic reductions in infectious rotavirus titer and four- and a five-fold increase in the Ct values compared to the virus control, respectively (p-value Conclusion: Our study suggests that in addition to cardiovascular diseases, calcium channel blockers at their optimal doses may also be used to treat gastroenteritis caused by rotavirus infection

Background: Peritoneal adhesions (PA) are a common complication of abdominal operations. Previous studies indicate that inhibition of inflammation and fibrosis at sites of peritoneal damage may prevent the development of intra-abdominal adhesions. Zingiber officinalis Roscoe (ginger) and Panax ginseng (P. ginseng) are herbal products with antioxidant and anti-inflammatory effects that can have restorative properties. Objective: This research aimed to examine the impact of ginger and p. ginseng on prevention of PA in a rat model after surgery. Methods: Following a laparotomy, the wall of the cecum was rubbed to induce intra-abdominal adherence in Wistar rats. Ginger (400mg/kg) and P. ginseng (500mg/kg) were orally adminstered to the animals. The animals were sacrificed on the 10th day after surgery, and the Nair and Leach scoring system was used to assess adhesion. The microscopic histology of the induced cecal adhesions was evaluated. An enzyme-linked immunosorbent assay (ELISA) was used to determined tissue levels of transforming growth factor-beta (TGF-β) on homogenized PA tissue. Real-time PCR was performed to quantify the mRNA expression of IL-1β, TNF-α, Col 1a1, and Col 3a1 in rat tissue. Result: The adhesion score and histopathological rating based on the Nair and Leach scoring criteria showed lower adhesion scores in the group of rats treated with P. ginseng compared to the control group (p Conclusion: The study's outcome recommends that P. ginseng could be an effective agent for preventing the PA and inflammation during the post-operative stage.

Objective: Hepatocellular carcinoma is one of the leading causes of cancer-related death worldwide. Experimental studies reported that some plants in the genus of Psoralea (Fabaceae family) show anticancer potential. The present study aimed to evaluate the effects of Psoralea drupacea extract (PDE) on HepG2 liver cancer cells. Methods: The proliferation, cell cycle, and migration of HepG2 cells were determined by thiazolyl blue tetrazolium bromide test, propidium iodide staining, and scratch assay, respectively. The effects of PDE on the activity of matrix metalloproteinases (MMPs) and angiogenesis were evaluated by the gelatin zymography method and chicken chorioallantoic membrane model, respectively. Results: The culture of HepG2 cells in the presence of PDE (24 hr and 48 hr) significantly reduced their viability (at a concentration of ≥ 50 µg/mL) and increased the percentage of cells in the sub-G1 stage. PDE also increased the antiproliferative and proapoptotic activities of doxorubicin (3 and 6 µg/mL). The extract significantly decreased the generation of reactive oxygen species and lipid peroxidation in the cells. Moreover, PDE (25 and 50 µg/mL) significantly suppressed the migration ability of HepG2 cells, which was associated with inhibition in the activity of MMP2 and MMP9 (50 µg/mL). Furthermore, treatment with PDE significantly reduced the number and diameter of vessels in the chick chorioallantoic membrane. Conclusion: PDE decreased the survival and cell cycle progression of liver cancer cells through a mechanism other than oxidative stress. This extract also showed an anti-angiogenesis effect and diminished the migration ability of HepG2 cells by inhibiting MMP activity

Abstract: Membrane technology as one of the most efficient and cost-effective separation methods has been mainly considered for different applications. But as with any method, there are some special drawbacks in membranes such as fouling, concentration polarization, and durability, which can limit the membrane performance in terms of permeate flux and solute rejection. Superamphiphilic polymers have different applications in different fields which include liquid-liquid separation membranes, self-healing, antifouling, self-cleaning, and adsorbent coatings. Recently, these kinds of coatings were applied on different membrane systems and the effects of coating on the membrane performance were evaluated. As a review, an overview on the most common methods for manufacturing membranes with superamphiphilic surface, a general review of the explored practical applications of such coatings in membranes, conclusions about the current situation of research, and commercial applications of superamphiphilic membranes are presented in this study.

Objective: Reactive oxygen species (ROS) are produced during cryopreservation of human sperm and impair sperm function. Antioxidant compounds, such as fennel and purslane, reduce the damaging effects of ROS. This study aimed to evaluate motility parameters, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), intracellular ROS, and DNA damage to determine the optimum concentrations of hydroalcoholic extracts of fennel and purslane for human spermatozoa cryopreservation.Methods: Twenty human sperm samples were used and divided into seven equal groups consisting of fennel hydroalcoholic extract (5, 10, and 15 mg/L), purslane hydroalcoholic extract (25, 50, and 100 mg/L), and no additive.Results: Supplementation of 25 mg/L and 50 mg/L purslane extract and 10 mg/L fennel extract in cryopreservation extender significantly increased the motility and PMI of sperm with a significant reduction in intracellular ROS compared to control groups (pp

Background: Curcumin has been isolated from the rhizomes of Curcuma longa. Over the years, it has shown outstanding therapeutic potential in various human disorders, including cancers. Objective: The aim is to study curcumin’s effects on the apoptosis signaling pathway in the head and neck squamous cell carcinoma (HNSCC) cell line HN5. Methods: The cytotoxicity of curcumin on HN5 cells were assessed. In addition, HN5 cells were also treated with curcumin to evaluate its effect on the caspase-8, -9, Bcl-2, Bax, and Stat3 gene expressions. Results: The results exhibited that cell viability reduced following curcumin treatment in a concentration- dependent manner. Curcumin treatment caused decreased expression of Bcl2, with simultaneous upregulation of the Bax/Bcl2 ratio. Curcumin increased caspase-9 expression, did not affect caspase-8, and decreased Stat3 expression. The induction of the mitochondria-dependent apoptosis pathway of curcumin happened by modulating the expression of Bcl2 and Bax genes, resulting in the caspase-9 activation. Furthermore, curcumin decreased the expression of the Stat3 in HN-5 cells. Conclusions: In conclusion, curcumin showed marked anticancer effects in the HN-5 cell line by modulating Stat-3; Bax/Bcl-2 expression in vitro.

Android is a highly fragmented platform with a diverse set of devices and users. To support the deployment of apps in such a heterogeneous setting, Android has introduced dynamic delivery —a new model of software deployment in which optional, device- or user-specific functionalities of an app, called Dynamic Feature Modules (DFMs) , can be installed, as needed, after the app’s initial installation. This model of app deployment, however, has exacerbated the challenges of properly testing Android apps. In this article, we first describe the results of an extensive study in which we formalized a defect model representing the various conditions under which DFM installations may fail. We then present DeltaDroid —a tool aimed at assisting the developers with validating dynamic delivery behavior in their apps by augmenting their existing test suite. Our experimental evaluation using real-world apps corroborates DeltaDroid ’s ability to detect many crashes and unexpected behaviors that the existing automated testing tools cannot reveal.

Mephedrone, a synthetic derivative of cathinone, is a commonly used psychoactive substance. Our previous study showed that exposure to mephedrone during pegnancy induced antiproliferative and pro-apoptotic effects in hippocampus of mice delivered pups. However, its effects on neural stem/progenitor cells (NS/PC) remain unexplored. The aim of this study is to investigate the effects of mephedrone exposure on the proliferation, differentiation, and apoptosis of rat embryonic NS/PC. NS/PC were isolated from rat fetal ganglionic eminence region at embryonic day 14.5. The effects of mephedrone on cell proliferation, neurosphere formation (colonies of NS/PC), neuronal differentiation, and apoptosis of NS/PC were assessed using MTT, immunocytochemistry, and flow cytometry. Mephedrone at concentrations of 20–640 µM significantly decreased the proliferation of NS/PC, induced cell cycle arrest, and enhanced the percent of apoptotic and necrotic cells. Neurosphere assays revealed a significant reduction in the number and diameter of neurosphere-forming cells. In addition, mephedrone significantly decreased the expressions of DCX and NeuN neuronal markers. Taken together, our results suggeste that exposure to mephedrone decreases the viability and neuronal differentiation of embryonic NS/PC. This study showed that mephedrone exposure during fetal or neonatal life may impair neurogenesis and subsequent brain development.

Infertility in women can be caused by various female reproductive diseases such as premature ovarian failure (POF), polycystic ovary syndrome (PCOS), endometriosis and Asherman syndrome that affect couples' quality of life and lead to mental, emotional, and physical problems. In recent years, clinical researchers have sought infertility treatments using new methods that are more effective and non-invasive than the old methods. Today, stem cell-based therapy has been introduced as a promising method and an alternative to the old strategy of infertility treatment. Understanding the main features and functional perspective of mesenchymal stem cells (MSCs) in the future of infertility by physicians is crucial. Mesenchymal stem cells (MSCs) are multipotent stem cells with a high proliferation range, abundant source and multidirectional differentiation potential. They have high potential for the treatment of injured tissues in regenerative medicine through cell homing, secretion of active factors, and participation in immune regulation. At present, due to fewer ethical restrictions on the use of mesenchymal stem cells compared to embryonic stem cells, more attention has been paid to these cells as a new treatment for gynecological disorders. In this paper, we first review the various type of female reproductive disorders along with their common treatment methods, then we evaluate the recent advances in the application of MSCs in the diseases related to infertility and improve the reproductive health of women worldwide.

Aim: We aimed to investigate the associated risk factors of deep vein thrombosis (DVT) among COVID-19 patients. Materials & methods: In this cross-sectional study the demographical data and clinical characteristics of 382 COVID-19 patients were collected and analyzed. Results: The DVT was observed in 53 patients (14.1%). The rate of death was significantly associated with the incidence of DVT, 48.1 versus 32.2% in non-DVT cases; p = 0.034). Also, BMI (p = 0.0001), renal failure (p = 0.001), lower-limb edema (p = 0.0001) and intubation (p = 0.004) were associated with the risk of DVT. Conclusion: COVID-19 patients with a higher BMI, renal failure, lower-limb edema and need for intubation were at a higher risk of DVT.

Due to the increasing expansion of renewable energy, especially the widespread installation of solar power plants worldwide, to better exploit and increase the efficiency and quality of power generation, we need to closely monitor the performance of important components of the plant. In this study, using an innovative smart monitoring system and electronic sensors, we monitored components such as power in photovoltaic (PV) arrays in real time, including the phenomenon of hot spots as an example of power loss in PV panels. Detection of hot spots is very difficult to deal with in a large power plant. The results also demonstrated smart monitoring that increased detection speed and increased the efficiency rate per supervisory technician by ≤36% compared with the previous efficiency rate, which was 10% per technical staff, and increased the quality of the operation of each solar power plant. In this paper, meteorological data were coordinated with research data to validate the research. Furthermore, to compare the results of using the smart monitoring method with the conventional observation method, a complete diagram of a 5-kW solar system in MATLAB® 2018 was simulated and output diagrams were presented. Finally, to provide a comprehensive validation, our research results were compared with technical data obtained from a local 5-kW solar power plant located in Sari, Iran (36°33ʹ48″N, 53°03ʹ36″E) with an average annual irradiation of 1490 kWh/m2. When the simulation results and research are analysed, it is clear that the smart and real-time monitoring approach brings various benefits to solar power plants.

A petrophysical model describing spectral induced polarization has been developed for clay-rocks accounting for the Maxwell Wagner polarization. It is also used to connect the complex conductivity to the relative permeability of the material. This model is applied to the Callovo-Oxfordian (COx) clay-rock of the Paris Basin (France) where the Meuse/Haute-Marne Underground Research Laboratory (URL) is located. Laboratory experiments are performed using 8 clay-rock cores to study the effect of desiccation on their spectral induced polarization response. The measurements are performed along the foliation plane. Complex conductivity spectra are performed over the frequency range 1 mHz to 45 kHz. These spectra are fitted with a double Cole Cole model to extract the evolution of the Cole Cole parameters with the saturation during the desiccation process. The low-frequency Cole Cole model corresponds to induced polarization phenomena while the high-frequency Cole Cole model corresponds to the Maxwell-Wagner contribution. We obtain the value of the first and second Archie’s exponents and we check the relationship between the surface conductivity and the cation exchange capacity of the clay-rocks. We are also able to connect the relative permeability curve to the second (saturation) Archie’s exponent. The monitoring of the complex conductivity can be used to predict how the permeability of the clay-rock formation changes with the water content.

This article contributes to the debate on the effect of social media usage on political attitudes. Our research question is: Does the use of social media affect citizens’ political trust? We test this research question using Egypt’s Arab Barometer Wave V country data (2018–2019). We expect social media usage to increase political distrust among citizens through the three mediating variables: corruption perceptions, perceptions of poor government performance, and perceptions of missing guaranteed freedoms. Our quantitative study results confirm these mediating relationships; that is, social media usage significantly increases political distrust among Egyptian citizens by increasing corruption perceptions, perceptions of poor government performance, and perceptions of a lack of guaranteed freedoms.

Purpose The purpose of this study is to investigate heat transfer and electrokinetic non-Newtonian flow in a rectangular microchannel in the developed and transient states. Design/methodology/approach The Carreau–Yasuda model was considered to capture the non-Newtonian behavior of the fluid. The dimensionless forms of governing equations, including the continuity equation for the Carreau–Yasuda fluid, are numerically solved by considering the volumetric force term of electric current (DC). Findings The impact of pertinent parameters such as electrokinetic diameter (R), Brinkman number and Peclet number is examined graphically. It is observed that for increasing R, the bulk velocity decreases. The velocity of the bulk fluid reaches from the minimum to the maximum state across the microchannel over time. At the electrokinetic diameter of 400, the maximum velocity was obtained. Temperature graphs are plotted with changes in the various Brinkman number (0.1 < Br < 0.7) at different times, and local Nusselt are compared against changes in the Peclet number (0.1 < ℘e < 0.5). The results of this study show that by increasing the Brinkman number from 0.25 to 0.7, the temperature along the microchannel doubles. It was observed that increasing the Peclet number from 0.3 to 0.5 leads to 200% increment of the Nusselt number along the microchannel in some areas along the microchannel. The maximum temperature occurs at Brinkman number of 0.7 and the maximum value of the local Nusselt number is related to Peclet number 0.5. Over time in the transient mode, the Nusselt number also decreases along the microchannel. By the increasing of time, the temperature increases at given value of Brinkman, which is insignificant at Brinkman number of 0.1. The simulation results have been verified by Newtonian and non-Newtonian flows with adequate accuracy. Originality/value This study contributes to discovering the effects of transient flow of electroosmotic flow for non-Newtonian Carreau–Yasuda fluid and transient heat transfer through rectangular microchannel. To the authors’ knowledge, the said investigation is yet not available in existing literature.

Machine-aided geological interpretation provides an opportunity for rapid and data-driven decision-making. In disciplines such as geostatistics, the integration of machine learning has the potential to improve the reliability of mineral resources and ore reserve estimates. In this study, inspired by existing geostatistical approaches that use radial basis functions to delineate domain boundaries, we reformulate the problem into a machine learning task for automated domain boundary delineation to partition the orebody. We use an actual dataset from an operating mine (Driefontein gold mine, Witwatersrand Basin in South Africa) to showcase our new method. Using various machine learning algorithms, domain boundaries were created. We show that based on a combination of in-discipline requirements and heuristic reasoning, some algorithms/models may be more desirable than others, beyond merely cross-validation performance metrics. In particular, the support vector machine algorithm yielded simple (low boundary complexity) but geologically realistic and feasible domain boundaries. In addition to the empirical results, the support vector machine algorithm is also functionally the most resemblant of current approaches that makes use of radial basis functions. The delineated domains were subsequently used to demonstrate the effectiveness of domain delineation by comparing domain-based estimation versus non-domain-based estimation using an identical automated workflow. Analysis of estimation results indicate that domain-based estimation is more likely to result in better metal reconciliation as compared with non-domained based estimation. Through the adoption of the machine learning framework, we realized several benefits including: uncertainty quantification; domain boundary complexity tuning; automation; dynamic updates of models using new data; and simple integration with existing machine learning-based workflows. Funder: Department of Science and Innovation (DSI)-National Research Foundation (NRF) Thuthuka Grant (UID: 121973); DSI-NRF CIMERA; Wits Mining Institute (WMI)

Abstract: Non-coding RNAs (ncRNAs) are emerging as important regulators in various pathological conditions, including human cancers. NcRNAs exert potentially crucial effects on cell cycle progression, proliferation, and invasion in cancer cells by targeting various cell cycle-related proteins at transcriptional and post-transcriptional levels. As one of the key cell cycle regulatory proteins, p21 is involved in various processes, including the cellular response to DNA damage, cell growth, invasion, metastasis, apoptosis, and senescence. P21 has been shown to have either a tumor-suppressive or oncogenic effect depending on the cellular localization and posttranslational modifications. P21 exerts a significant regulatory effect on both G1/S and G2/M checkpoints by regulating the function of cyclin-dependent kinase enzymes (CDKs) or interacting with proliferating cell nuclear antigen (PCNA). P21 has an important effect on the cellular response to DNA damage by separating DNA replication enzymes from PCNA and inhibiting DNA synthesis resulting in G1 phase arrest. Furthermore, p21 has been shown to negatively regulate the G2/M checkpoint through the inactivation of cyclin-CDK complexes. In response to any cell damage caused by genotoxic agents, p21 exerts its regulatory effects by nuclear preservation of cyclin B1-CDK1 and preventing their activation. Notably, several ncRNAs, including lncRNAs and miRNAs, have been shown to be involved in tumor initiation and progression through the regulation of the p21 signaling axis. In this review, we discuss the miRNA/lncRNA-dependent mechanisms that regulate p21 and their effects on gastrointestinal tumorigenesis. A better understanding of the regulatory effects of ncRNAs on the p21 signaling may help to discover novel therapeutic targets in gastrointestinal cancer.

Background: The COVID-19 pandemic has had a significant impact on people's psychological functioning, including how the cope with anxiety. This study aimed to assess the role of coping styles on the relationship of COVID-19 anxiety and Washing Obsessive-Compulsive Disorder (W-OCD) symptoms. Methods: A cross-sectional study was performed on 420 people living in Kashan city (Iran) from March to April 2020. Participants were selected by the convenience sampling method due to the difficulties brought about by COVID-19 and completed the contamination subscale of Padua Inventory, Covid-19 anxiety inventory, and coping strategies scale. Data was analyzed by structural equation modeling (SEM) using AMOS- 22. Results: The results revealed that emotion focused, somatization and social support coping strategies were significantly associated with W-OCD symptoms. Also, there was a significant correlation between the Covid-19 anxiety with the W-OCD symptoms. SEM result revealed that emotion focused and somatization coping strategies positively mediated the relationship between COVID-19 and W-OCD symptoms. Conclusion: Emotion focused and somatization coping strategies increase W-OCD symptoms following COVID-19 anxiety. Psycho education interventions addressing COVID-19's physical and psychological impacts on health, discriminating the rational and adaptive behavior and obsessive and compulsive behaviors, restricting the information gathering from numerous sources which may lead to increased negative emotions might be helpful.

Abstract: Type 2 diabetes (T2D), which affects many people around the world, is one of the diseases that is on the rise. Various studies have revealed that insulin resistance and lessened insulin production have been associated with T2D, and they also show that this disease can have a genetic origin and is associated with different genes, such as KCNQ1, PPAR-γ, calpain-10, ADIPOR2, TCF7L2, which can be utilized as therapeutic targets. Different therapeutic approaches and strategies such as exercise and diet, pharmacological approaches, and utilization of nanoparticles in drug delivery and gene therapy can be effective in the treatment and control of T2D. Glucagon-like peptide 1 (GLP-1) and sodiumglucose cotransporter-2 (SGLT2) have both been considered as drug classes in the treatment of T2D and T2D-related diseases such as cardiovascular disease and renal disease, and have considerable influences such as diminished cardiovascular mortality in individuals with T2D, ameliorated postprandial glycaemia, ameliorated fasting glycaemia, and diminished bodyweight on disease treatment and improvement process. In the present review article, we have attempted to explore the risk factors, genes, and diseases associated with T2D, therapeutic approaches in T2D, the influences of drugs such as dapagliflozin, metformin, acarbose, Januvia (sitagliptin), and ertugliflozin on T2D in clinical trials and animal model studies. Research in clinical trials has promising results that support the role of these drug approaches in T2D prophylaxis and ameliorate safety even though additional clinical research is still obligatory.

Funding Information: We want to thank all cooperation associates in the framework of this manuscript. Further, we especially thank the Institut Laue-Langevin for the synthesis of the TMPT materials and Gabriele Es-Samlaoui for the preparation of TEM samples used in this work. This work was supported by the German Science Foundation (DFG), project CRC 1279 (project number 316249678), projects KR 4866/8-1, and the collaborative research center “Chemistry of Synthetic 2D Materials” SFB-1415-417590517. This work was supported by a project funded by the Carl Zeiss foundation. The computational support from the Technical University of Dresden computing cluster (TAURUS) and High-Performance Computing Center (HLRS) in Stuttgart is gratefully appreciated. M.K.K. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG) within the project DFG: KI 2546/1-1. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society. Quasi-two-dimensional (2D) manganese phosphorus trisulfide, MnPS3, which exhibits antiferromagnetic ordering, is a particularly interesting material in the context of magnetism in a system with reduced dimensionality and its potential technological applications. Here, we present an experimental and theoretical study on modifying the properties of freestanding MnPS3 by local structural transformations via electron irradiation in a transmission electron microscope and by thermal annealing under vacuum. In both cases we find that MnS1-xPx phases (0 ≤ x < 1) form in a crystal structure different from that of the host material, namely that of the α- or γ-MnS type. These phase transformations can both be locally controlled by the size of the electron beam as well as by the total applied electron dose and simultaneously imaged at the atomic scale. For the MnS structures generated in this process, our ab initio calculations indicate that their electronic and magnetic properties strongly depend on both in-plane crystallite orientation and thickness. Moreover, the electronic properties of the MnS phases can be further tuned by alloying with phosphorus.

Background: Intranasal oxytocin can be used as a promising moiety for the treatment of sexual disorders. Objective: This study was carried out to systematically review the effect of intranasal oxytocin on sexual function in men and women Methods: We systematically searched databases (e.g., Cochrane Central Register of Controlled Trials Library, MEDLINE, Web of Science, Scopus, ProQuest, Google Scholar and Persian databases). All types of published clinical trials comparing different doses of intranasal oxytocin sprays with placebo sprays were included in the study. The primary outcome was sexual function and secondary outcomes were endocrine and cardiovascular measures and also side effects Results: A total of six studies were ultimately eligible for inclusion in the study. Though intranasal oxytocin improves various parameters of sexual function in men and women, according to the sexual response cycle, these changes are not statistically meaningful compared to the control group. Only one study revealed a meaningful impact on orgasm parameters and after orgasm, especially in men. In all studies, intranasal oxytocin administration has significantly and transiently increased plasma concentrations of oxytocin with no meaningful effect on other endocrine hormones. A study showed that the heartbeat is increased transiently during the arousal and orgasm stages, and such increase is meaningfully higher in men than in women. Conclusion: Intranasal oxytocin administration fails to meaningfully affect the classical parameters of sexual response, but it improves the orgasmic and post-orgasmic dimensions, especially in men. To evaluate the effects of intranasal oxytocin administrations, we need more long-term clinical trials.