1. Hyposoter didymator uses a combination of passive and active strategies to escape from the Spodoptera frugiperda cellular immune response
- Author
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Serge Urbach, Eric Wajnberg, Anne-Nathalie Volkoff, Véronique Jouan, Marc Ravallec, Tristan Dorémus, Patrick Wincker, François Cousserans, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 2 - Sciences et Techniques (UM2), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Genoscope ('Polydnavirus' projet), ANR-09-BLAN-0243-01, Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), ANR-09-BLAN-0243,PARATOXOSE(2009), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)
- Subjects
0106 biological sciences ,Proteome ,oviduct ,Physiology ,système immunitaire ,ichnovirus ,Wasps ,mass spectrum analysis ,Egg protein ,venom ,parasitisme des oeufs ,apolipoprotéine ,Polymerase Chain Reaction ,01 natural sciences ,follicule ovarien ,ichneumonidae ,Tandem Mass Spectrometry ,spectrométrie de masse ,hyposoter didymator ,symbiote ,fluid ,Immunity, Cellular ,0303 health sciences ,Polydnavirus ,fluide ,mode de vie ,Cell biology ,symbiont ,protéine ,Larva ,Insect Proteins ,Female ,polydnavirus ,hymenoptera ,protéine de synthèse ,Braconidae ,food.ingredient ,particule virale ,apolipoprotein ,venin ,Spodoptera ,Biology ,sepals ,Calyx ,braconidae ,évasion immune ,Viral Proteins ,03 medical and health sciences ,life styles ,Immune system ,food ,calice ,Animals ,oviducte ,Ovum ,030304 developmental biology ,Egg Proteins ,Virion ,protéines de surface ,biology.organism_classification ,Virology ,010602 entomology ,ovarian follicle ,Polydnaviridae ,Insect Science ,encapsulation ,réponse de l'hôte ,Ichnovirus ,aphidiidae ,endoparasite ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,protein - Abstract
An endoparasitic life style is widespread among Hymenoptera, and various different strategies allowing parasitoids to escape from the host encapsulation response have been reported. Species carrying polydnaviruses (PDVs), such as the ichneumonid Hyposoter didymator, generally rely on the viral symbionts to evade host immune responses. In this work, we show that H. didymator eggs can evade encapsulation by the host in the absence of calyx fluid (containing the viral particles), whereas protection of the larvae requires the presence of calyx fluid. This evasion by the eggs depends on proteins associated with the exochorion. This type of local passive strategy has been described for a few species carrying PDVs. Immune evasion by braconid eggs appears to be related to PDVs or proteins synthesized in the oviducts being associated with the egg. We report that in H. didymator, by contrast, proteins already present in the ovarian follicles are responsible for the eggs avoiding encapsulation. Mass spectrometry analysis of the egg surface proteins revealed the presence of host immune-related proteins, including one with similarities with apolipophorin-III, and also the presence of three viral proteins encoded by IVSPERs (Ichnovirus Structural Protein Encoding Regions).
- Published
- 2013
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