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Humans have been intrigued by phytochemicals from the Syzygium genus (Myrtaceae) for thousands of years due to their diverse applications. The biological activity of plants is varied, including anti-inflammatory, diuretic, antifungal, antibacterial, and antimicrobial effects. There have been no reports on the phytochemical profiling of the Southern Ghats endemic, Syzygium laetum. This study aimed to examine the phytoconstituents of S. laetum leaves. The GC-MS analysis of S. laetum leaves chromatograms revealed the presence of eleven peaks including Methyl 2-hydroxy-octadeca-9,12,15-trienoate, 2,2-Dimethyl-3-vinyl-bicyclo[2.2.1]heptane, Lupeol, Cholest-4-en-3-one, and 3-Pentadecylphenol. Phytochemical analysis reveals the existence of several pharmacologically active bioactive compounds, such as alkaloids, tannins, glycosides, phenolics, carbohydrates, essential oil, reducing sugar, and saponins, and that might be responsible for their bioactivity.

Chemical properties of red petiole Curry leaf (Murraya koenigii) by GCMS analysis

Chinese betel leaf (Perperomia pellucida) is generally widely used as a medicinal plant. Many people use Chinese betel leaf as a traditional medicine based on natural ingredients which are believed to be able to treat various diseases with fewer side effects than synthetic drugs. In general, China betel leaf has several secondary metabolites, generally flavonoids, steroids, alkaloids and terpenoids. However, in testing for secondary metabolites, there is still no clear description of the plant parts taken. So this study aims to determine the secondary metabolites in the young leaves of Chinese betel (Peperromia pellucida) by gas chromatography. The results obtained based on the GCMS analysis contained 18 secondary metabolites. Phytol, 2,3-BIS (4-(CA RBOXYMETHYL) PHENYL) BENZO [B] FURAN, and 6-ALLYL-4,5 -DIMETHOXY- 1,3-BENZOD IOXOLE are the 3 highest bioactive com pounds based on the percentage of content.

Saat ini senyawa penanda yang diperlukan sulit diperoleh di peredaran karena termasuk senyawa dilarang di Indonesia. Penelitian ini dilakukan untuk mengembangkan alternatif metode tanpa baku pembanding dengan pendekatan profil kandungan senyawa penanda menggunakan spektrometer massa. Spektrometri massa dilakukan baik menggunakan gas kromatografi maupun liquid chromatography high resolution mass spectrometry (LC-HRMS). Kedua metode tersebut dikembangkan untuk uji konfirmasi identitas senyawa penanda pada tanaman kratom. Uji spektrometri massa dilakukan setelah senyawa penanda diekstraksi dari tanaman. Uji gas kromatografi dilakukan menggunakan mode selected ion monitoring (SIM) pada m/z 214, 397, 383, dan 269. Sistem kromatografi LC-HRMS menggunakan modifier ion asam formiat 0.1% dalam air dan asetonitril sebagai fase gerak. Analisis dilakukan menggunakan spektrometer massa quadrupole time of flight (QToF) sebagai mass analyzer, mode ionisasi ESI+ dan mode scan. Analisis kualitatif menggunakan data waktu retensi (Rt) dan fragmen ion senyawa penanda tanaman kratom. Diperoleh 3 (tiga) senyawa penanda tanaman kratom, yaitu speciociliatine, speciogynine, dan mitragynine. Uji spesifisitas dilakukan dengan membandingkan waktu retensi yang dikonfirmasi dengan nilai Retention Index (RI) secara GCMS serta nilai m/z senyawa penanda secara LC-HRMS QToF. Hasil analisis menunjukkan bahwa 3 (tiga) senyawa penanda utama dapat digunakan dalam identifikasi tanaman kratom. Metode ini dapat diaplikasikan sebagai uji konfirmasi kandungan tanaman kratom dalam obat tradisional dimana metode LC-HRMS QToF merupakan meotode yang lebih sensitif dibandingkan dengan GCMS dengan nilai LOD 149.2 µg/g dan 67.6 mg/g sehingga dapat digunakan sebagai metode konfirmasi setelah sampel diekstraksi dan diisolasi secara KLT.

Climate change is one of the leading issues affecting river basins due to its direct impacts on the cryosphere and hydrosphere. General circulation models (GCMs) are widely applied tools to assess climate change but the coarse spatial resolution of GCMs limit their direct application for local studies. This study selected five CMIP5 GCMs (CCSM4, HadCM3, GFDL-CM3, MRI-CGCM3 and CanESM2) for performance evaluation ranked by Nash–Sutcliffe coefficient (NSE) and Kling–Gupta Efficiency (KGE). CCSM4 and HadCM3 large-scale predictors were favored based on ranks (0.71 and 0.68, respectively) for statistical downscaling techniques to downscale the climatic indicators Tmax, Tmin and precipitation. The performance of two downscaling techniques, Statistical Downscaling Methods (SDSM) and Long Ashton Research Station Weather Generator (LARS-WG), were examined using the Mean Absolute Error (MAE), Root Mean Square Error (RMSE), bias, NSE and KGE with weights (Wi) for the validation period. The results of statistical measures proved SDSM more efficient (0.67) in comparison to the LARS-WG (0.51) for the validation time for the Jhelum River basin. The findings revealed that the SDSM simulation for Tmax and Tmin are more comparable to the reference data for the validation period except simulation of extreme events by precipitation. The 21st century climatic projections exhibited a significant rise in Tmax (2.37–4.66 °C), Tmin (2.47–4.52 °C) and precipitation (7.4–11.54%) for RCP-4.5 and RCP-8.5, respectively. Overall, the results depicted that winter and pre-monsoon seasons were potentially most affected in terms of warming and precipitation, which has the potential to alter the cryosphere and runoff of the Jhelum River basin.

Tandan kosong sawit (TKS) adalah limbah hasil dari proses pengolahan minyak sawit yang terdiri dari serat dan kulit yang masih mengandung minyak. TKS dapat digunakan sebagai bahan bakar pembangkit listrik atau dalam proses pirolisis untuk menghasilkan bio-oil, biogas, dan biochar. Penelitian ini adalah untuk menganalisis komposisi kimia dan menentukan profil karakteristik tandan kosong sawit menggunakan metode FT-IR dan Pirolisis-GCMS. Ini membantu untuk memahami lebih baik tentang kualitas tandan kosong sawit dan potensinya sebagai sumber bahan baku. Hasil uji menunjukkan kandungan kimia TKS yang beragam dan gugus-gugus fungsional seperti hidrokarbon, alkohol, aldehyde, asam, hidroksi, karboksilat, keton, klorida, nitrat, nitril, amina, fenol, hidrogen sulfida, sulfur, dan oksigen. Nilai kalor TKS bervariasi antara 4.000 - 4.400 kkal atau 16,6 - 18,48 kJ per metrik ton. Pirolisis pada suhu 400oC menghasilkan senyawa yang memiliki gugus hidrokarbon alifatik dan asiklik, serta kandungan metana yang lebih tinggi.

We present four 140-yr wind-wave climate simulations (1961–2100) forced with surface wind speed and sea ice concentration from two CMIP6 GCMs under two different climate scenarios: SSP1–2.6 and SSP5–8.5. A global three-grid system is implemented in WAVEWATCH III to simulate the wave–ice interactions in the Arctic and Antarctic regions. The models perform well in comparison with global satellite altimeter and in situ buoys climatology. The comparison with traditional trend analyses demonstrates the present GCM-forced wave models’ ability to reproduce the main historical climate signals. The long-term datasets allow a comprehensive description of the twentieth- and twenty-first-century wave climate and yield statistically robust trends. Analysis of the latest IPCC ocean climatic regions highlights four regions where changes in wave climate are projected to be most significant: the Arctic, the North Pacific, the North Atlantic, and the Southern Ocean. The main driver of offshore wave climate change is the wind, except for the Arctic where the significant sea ice retreat causes a sharp increase in the projected wave heights. Distinct changes in the wave period and the wave direction are found in the Southern Hemisphere, where the poleward shift of the Southern Ocean westerlies causes an increase in the wave period of up to 5% and a counterclockwise change in wave direction of up to 5°. The new CMIP6 forced wave models improve in performance compared to previous CMIP5 forced wave models, and will ultimately contribute to a new CMIP6 wind-wave climate model ensemble, crucial for coastal adaptation strategies and the design of future marine offshore structures and operations. Significance Statement The purpose of this study is to advance the understanding of ocean wind-wave climate evolution over the twentieth and twenty-first centuries and to effectively communicate the long-term impacts of climate change in diverse wind-wave climatic regions across the globe. The 140-yr continuous model results produced in this work are crucial to studying changes in extreme sea states and investigating the relationship between interdecadal periodic oscillations and long-term climate trends. The dataset produced can be used to gain further insight into the substantial long-term changes of the polar wind-wave climate caused by the rapid decrease of sea ice coverage, and the evolution of the directional changes in the sea states triggered by climate change.

Endoparasites, namely, Ascaris sp., Taenia sp., Haemonchus contortus, Ancylostoma duodenale etc. are of serious concern since they can lead to financial loss if farm animals are attacked by these parasites. Finding out cost-effective natural remedies for these infections is an area of research in the field of veterinary sciences. The current study was undertaken with a view to explore the anthelmintic property of banana peel along with its other bioactive properties. The fruit is available abundantly throughout the year and the peel is often discarded as waste. This study has shown that the extracts of the Nendran variety of Musa paradisiaca L. fruit peel have potent anthelmintic, antibacterial and antifungal activities. Preliminary cytotoxicity studies have also given positive results. GCMS analysis has revealed the major phytochemicals responsible for the bioactive properties of the peel. Since the United Nations has urged countries to align research and development with a thrust on sustainable development, these kinds of natural alternatives are best suited in place of synthetic drugs.

This paper presents a cost-effective and validated multi residue modified and miniaturized method for the determination of 220 chemically different groups of pesticides and their isomers. This determination method is performed with single Quaid Gas Chromatography Mass Spectrometry -Total Ion Chromatogram GCMS-TIC. Two methods was experimented and modified with different GCMS parameters to analyses most common used pesticide and their residues in the standers solution and can be applied for real environmental samples. The results showed by single Quaid GCMS-TIC it can analyze 220 pesticides including their isomers within 49.6 min and low detection limit by using modified method 2 as described in this research. Limit of detection (LOD) was ranged from 0.78 to 14.74 ng/ml (ppb) with good separation and resolution. Limit of quantification (LOQ) was ranged between 2.34 and 44.22 ng/ml (ppb). Method 2 was more accurate, shorter, and clear separation rather than method 1. This method can be successfully applied in real environmental samples proven to be a good option for routine analysis of pesticide within the maximum residue limits (MRL) referenced to European commission especially with the most common GCMS-TIC which exists in most of labs and low income countries.

Suhu kolom Gas Chromatography-Mass Spectrometry (GCMS) merupakan salah satu variabel yang harus diperhatikan pada analisis senyawa kimia, agar senyawa terpisahkan dengan baik. Penelitian ini bertujuan untuk menentukan suhu terprogram optimum pada analisis asam lemak menggunakan instrumen GCMS. Sampel asam lemak yang digunakan berasal dari hasil ekstrak mikroalga Chlorella. Ekstrasi mikroalga Chlorella dilakukan dengan cara sonikasi selama 60 menit menggunakan pelarut n-heksana. Variasi suhu terprogram GCMS yang dilakukan, yaitu kenaikan suhu masing-masing pada 5 OC/menit, 7 OC /menit, 9 OC /menit, 12 OC /menit dengan suhu awal 80 OC dan suhu akhir 270 OC. Jumlah sampel yang diinjeksikan sebanyak 0,2 µL. Data hasil analisis diolah dengan cara membandingkan waktu retensi dan lama waktu analisis. Ditemukan, kondisi optimum suhu terprogram pengukuran, yaitu suhu awal 80 OC dan kenaikan 9 OC/menit sampai suhu 270 OC dengan lama waktu analisis 21,1 menit. Asam lemak yang terkandung dalam ekstrak mikroalga Chlorella yaitu asam palmitat, asam oleat dan asam stearat. Dengan menggunakan metode pengukuran ini waktu analisis menjadi lebih singkat dan senyawa yang diharapkan dapat teridentifikasi dengan baik. ; ; Kata kunci: Analisis, GCMS, suhu terprogram, asam lemak, mikroalga Chlorella.

Pyrolysis gas chromatography-mass spectrometry (Py-GCMS) is a simple, rapid, and reliable analytical technique to assay lignocellulose biomass. This study aims to analyze the chemical content of various ages of platinum fast-grown teak wood using PyGCMS coupled with interdependence multivariate analysis. Fifty-eight peaks were shown in the pyrogram originating from carbohydrates, lignin, and extractive derivatives. Along with age, platinum fast-grown teak wood showed higher carbohydrate derivatives in heartwood than sapwood. Compared to teak wood grown in conventional (between 36.72-45.69%), platinum fast-grown teak wood has a higher content of carbohydrates (42.82-48.04%). A substantial amount of G-unit lignin was detected in the sapwood, while the S-unit lignin dominated the heartwood. The extractive content of 10-years-old teak wood heartwood was 4.82%, higher than 10- and 20-years-old heartwood from conventional wood (2.23% and 8.88%, respectively). Multivariate analysis of the chemical compound showed that Py-GCMS could be utilized to classify platinum fast-grown and conventional teak wood. 2-methyl anthraquinone (MAQ) content of 10-year-old fast-grown teak wood was 2.5 times higher than 20-year-old conventional teak wood. Based on the study, platinum fast-grown teak wood is promising as alternative wood material to fulfill the market demand for conventional teak wood.

This study was conducted to investigate the bioactive and chemical components, ethnobotany and pharmacological activities of Calotropis procera leaves and fruits with a focus on its antimicrobial effect on isolated bacteria. To prepare the plant extract, methanol was used as the solvent, for evaluation of the bioactive compounds and their concentration in the plant extracts hence, qualitative and quantitative phytochemical analysis was conducted respectively. The GCMS analysis was conducted for further determination of the chemical composition of the plant parts. The antimicrobial impacts of the plant leaves and fruits methanolic extracts were carried out against assay organisms which were bacterial group and they include Standard and clinical strains of Bacillus subtilis ATCC 6633, Bacillus subtilis clinical, Staphylococcus aureus β-haemolytic strain and clinical, Staphylococcus aureus, Salmonella typhi clinical, Klebsiella pneumonia clinical, Escherichia coli and Proteus sp. Clinical strain. This study showed the presences of some phytochemical compounds such as tannin, alkaloid, saponin, flavonoid etc. in C. procera leaves and fruits at varying concentration. The GCMS analysis revealed that Pentadecanoic acid and n-Hexadecanoic acid were detected in both leaves and fruits of C. procera extracts. Furthermore, methanolic extract of the plant leaves had antibacterial effect potency towards Bacillus subtilis ATCC 6633, while the fruit extract had no antibacterial effect against the assay organism.

Climate change significantly impacts the natural systems, accelerating the global water cycle, and impacting various ecosystem services. However, the expected effects of climate change on the frequency and severity of extreme events on hydrological systems vary significantly with location. The present study investigates the uncertainties engulfed in hydrological predictions for the Tungabhadra River Basin. The ensemble streamflow projections were generated using four hydrological models, five climate models, and four climate scenarios to illustrate the associated uncertainties. The uncertainty in hydrological components such as streamflow (QQ), water availability (WA), and potential evapotranspiration (PET) was analysed in the future period (2015–2100). The results suggest that, in the monsoon period, precipitation projections increase by about 10.43–222.5%, whereas QQ projections show an increment between 34.50 and 377.7%. The analysis of variance (ANOVA) technique is used to further quantify the contribution of different sources to the total uncertainty. Furthermore, the ensemble spread is optimized using quantile regression forests (QRF), and the post-processed flows are likely to decrease up to 7% in June and increase up to 70% in September. This study is envisaged to give insights into the quantification of uncertainties in the prediction of future streamflow for rational and sustainable policymaking.

In order to analyze the potential bioactive constituents of leaf extracts of Prosopis Cineraria/ Khejri- National tree of Rajasthan, a plant with enormous Ayurvedic properties, Fourier Transform Infrared Spectroscopy (FTIR) along with Ultra Violet Spectroscopy (UV) and added Field Emission Scanning Emission Microscope with Energy Dispersive X-ray analysis (FESEM-EDAX) were carried out. The authors too demonstrated cell line studies via MTT Assay for MOLT 4, blood cancer in different concentrations and found Khejri as toxic with validated IC50. Further, Gas Chromatography-Mass Spectroscopy (GC-MS) and High-Performance Liquid Chromatography (HPLC) were tested and certain compounds gave spectrum with few major peaks concluded by other results, most of which are bioactive compounds which may act as antimicrobial, anti-cancerous, antioxidant, antiviral and anti-inflammatory agents. On an all, Khejri/Sami leaves of Mimosaceae family of Spunge tree is having anti cancerous properties for blood cancer that may repair punctured blood vessels if being treated properly. The authors have demonstrated the testing over country eggs with treated/ untreated group of eggs and found the formation of blood vessels that gesture how effective the drug is, being composed of Khejri extract. It is not only confined to cancer diseases but acts as a very good agent for inflammation, digestion and conjunctivitis. On an all, this natural compound so extracted is found to be useful for blood cancer with IC50 as 57.11 that simply reflect that the medicine composed from the same will be highly beneficial for cancer patients - let it be Ayurvedic, homoeopathic or allopathic.

The current study was conducted to examine the in vitro anticancer potential of Cordia dichotoma (bark, leaves, pulp and seed). The plant material was collected from UT of J&K and methodical bioassays were carried out on ten human cancer cell lines (Michigan Cancer Foundation-7 (MCF-7), M.D. Anderson-Metastatic Breast (MDA-MB-231), Neuroblastoma-2a (N2A), SH-SY5Y, U-251, HCT-116, SW-620, A-549, MIA PaCa-2, Panc-1) from five different origins (breast, CNS, colon, lung, pancreas) respectively. Methanolic extracts were produced and fractions were then obtained from the extracts and evaluated for cytotoxicity. Mechanistic assays, HPLC, and GCMS profiling were performed on the highest active fraction. The Sulforhodamine B (SRB) assay determined the in vitro cytotoxicity. The findings revealed that the bark portion had in vitro cytotoxicity against the A-549 human lung cancer cell line. To our knowledge, this is the first study to show that the plant’s bark has anticancer properties and induced chromatin condensation, confirmed cell death via ROS generation, and significantly decreased colony formation in A-549 cell line from lung origin in a dose-dependent manner. Furthermore, HPLC and GCMS investigations indicated the presence of a number of bioactive molecules such as gallic acid (144,969.86) uV*sec, caffeic acid (104.26) uV*sec, ferulic acid (472.87) uV*sec, vanillic acid (13,775.39) uV*sec, palmitic acid (18.34%), cis vaccenic acid (28.81%), etc. and one of the compounds was reported for the first time from the bark. As a result of its promising efficacy, it may become an essential cancer chemopreventive or chemotherapeutic medication for patients with lung carcinoma.

Complex matrices, such as wine, provide a challenge in the quantification of compounds. There exists a high likelihood of co-elution in these matrices, thereby artificially increasing the observed concentration. This can often lead to confusing data where compounds are above aroma detection thresholds, but are not detected by olfactory analysis. Additionally, the lack of sensitivity in assays can lead to the non-detection of sub-aroma threshold concentrations and contrasting data when olfactory analysis detects these aromas. To eliminate these pitfalls and gain a better understanding of the role that methoxypyrazines impart green character to wine, a quantitative method using headspace solid-phase microextraction coupled to heart-cutting multidimensional gas chromatography mass spectrometry was developed. The method can quantitate the three common methoxypyrazines found in wine at the picogram per liter level while resolving co-eluting compounds. The proposed method was validated using model wine and wine solutions and was ultimately used for the comparative analysis of white, ros&eacute, and red wines.

Culex quinquefasciatus (C. quinquefasciatus) is found in tropical and warm temperate regions and can transmit several viral diseases like Elephantiasis or Filariasis, West Nile fever, St. Louis encephalitis, and Japanese encephalitis causes millions of deaths every year. Long term use of synthetic pesticides to control mosquitoes, has led to the emergence of insecticide resistant vector, ecological imbalance, and harm to mammals, water contamination, toxicity to non-target organisms and residual effects. Therefore, plant based products are being searched and evaluated all over the world as an alternative control. The present study evaluated the efficacy of essential oil of Tagetes erecta (T. erecta) and Cymbopogon nardus (C. nardus) as a larvicidal, ovicidal, ovipositional deterrency and adulticidal agent against C. quinquefasciatus. Various bioassays against the IVth instar larvae, eggs and adults of C. quinquefasciatus were carried out. Five different concentrations of 10, 20, 40, 80,160, 320, 640 ppm was prepared for T. erecta whereas 20, 40, 80,160,320, 640, 1280 ppm concentrations were prepared for C. nardus essential oil to get LC50. Mortality data was calculated by Logprobit method of Finney using SPSS 16 (SPSS 2010) following Abbott method. The GCMS analysis of the essential oil of T. erecta and C. nardus was also conducted to estimate the presence of bioactive compunds in the essential oils. The essential oil of T. erecta and C.nardus, showed LC50 at 42.02 ppm and 160.29 ppm respectively after 24 hours. Whereas LC50 after 48 hrs is recorded at 39.25 ppm in T. erecta and 94.39 ppm in C. nardus against C. quinquefasciatus. At 40 ppm, T.erecta showed ovicidal activity with 40.99%, ovipositional activity with 57.80% and adulticidal activity with 88.00%. On the other hand, C. nardus at 160.29 ppm exhibited ovicidal activity with 77.63%, ovipositional activity with 73.33% and adulticidal activity with 73.6%. Many active phytochemicals was also found in the essential oil of T. erecta and C. nardus. It is concluded that the essential oil of both T. erecta and C. nardus are very effective mosquitocidal agent against C. quinquefasciatus. However T. erecta shows more effective results in comparision to C. nardus.

Background: It is a practice to use traditional medicine for curing various illnesses in West Africa. Acacia hebecladoides, Acacia albida and Gmelina arborea were selected based on their traditional belief for treating various ailments such as: hallucinations, inflammation and tuberculosis. The study sought to validate the antitubercular activity, antioxidant properties and phytochemical components of extracts and fractions of the leaves of selected plants. Method: The antitubercular activities of the plants were evaluated against Mycobacterium tuberculosis (MTB), multidrug-resistant Mycobacterium tuberculosis (MDR-TB) and non-mycobacterium tuberculosis (NTM) using the Lowenstein Jensen (LJ) proportion method. Gas chromatography mass spectrometer (GCMS) assay and the antioxidant assays (1,1- diphenyl -2-picryhydrazyl (DPPH) radical assay, total phenolic content (TPC), β-carotene bleaching activity and nitric oxide scavenging activity) were all carried out. Results: All three plant extracts used in the study inhibited Mycobacterium tuberculosis with G. arborea showing the lowest MIC value of 31.25μg/ml. Also, G. arborea inhibited MDR-TB with an MIC value of 125μg/ml. The most active plant, G. arborea was fractionated into four partitions, three (aqueous, n-hexane and ethyl acetate) fractions showed inhibitory activity against MTB with an MIC value of 62.5μg/ml. Also n-hexane and chloroform fractions of G. arborea showed inhibitory activities against MDR-TB with the lowest MIC value of 31.25μg/ml. The nitric oxide activity of the plant fractions showed that ethyl acetate fraction of G. arborea had high nitric oxide antioxidant activity. The ethanolic extract A. hebecladoides and G. arborea, while fractions of G. arborea (aqueous, ethyl acetate and chloroform) showed DPPH scavenging activity. The ethanolic extract A. hebecladoides and A. albida, while aqueous fraction of G. arborea had high total phenolic content. The ethanolic extract G. arborea and its fractions (aqueous and n hexane) showed β-carotene bleaching inhibition. Twelve compounds were found in the n-hexane fraction of G. arborea as 2,3-dihydro-3,5,-6-methy 4H-Pyran-4-one and 5-hydroxymethylfurfural were abundant with 35.81% and 19.03% respectively, followed by 3,5-dihydroxy-2-methy-4H-Pyran-4-one (9.82%) and n-Hexadecanoic acid (8.45%). Conclusions: The study carried out showed that ethanolic extract and fractions of G. arborea possess inhibitory activities against MTB and MDR-TB. Also ethyl acetate and aqueous fractions of G. arborea showed high antioxidant activities.

Marine algae are one of the largest sources for biogenic compounds. They produce a wide variety of chemically active metabolites. They are found to possess significant antioxidant, and phytochemical activities. Macro algae provide a great variety of metabolites and natural bioactive compounds with antimicrobial activity, such as polysaccharides, polyunsaturated fatty acids, phlorotannins and other phenolic compounds, and carotenoids. Several species of brown algae have been investigated for their bioactive natural products. In the present study the phytochemical, GCMS and Antioxidant assays such as ABTS assay and DPPH assays were carried out for Padina tetrastromatica using two different solvents ethanol and acetone. The phytochemical analysis showed the presence of alkaloids, steroids, and glycoside. GC-MS analysis of ethanol and acetone extracts of Padina tetrastromatica showed the presence of 35 compounds. For antioxidant activity, DPPH radical assay and ABTS radical cation scavenging assay were carried out.

Tomatoes are consumed worldwide as fresh vegetables because of their high contents of essential nutrients and antioxidant-rich phytochemicals. Tomatoes contain minerals, vitamins, proteins, essential amino acids (Leucine, Threonine, Valine, Histidine, Lysine, Arginine), monounsaturated fatty acids (Linoleic and Linolenic Acids), Carotenoids (Lycopene and β-Carotenoids) and Phytosterols (β-Sitosterol, Campesterol and Stigmasterol). GCMS analysis revealed the presence of 3-methylheptane, Ethylcyclohexane, 2-Methyl-4,6-octadiyn-3-one, 5,6-Dimethylundecane, (3E)3-Hexen-2-one, 2,2-Dimethylbutane, 1,2-Diphenyl-1-butanone, Isopropylbenzene (2-phenylpropane), 3,5-Dimethyloctane, 2-Phenyl-3-buten-1-ol, 2,4,4-Trimethylhexane, Benzoylcarboxaldehyde (Phenylglyoxal), Cis-3-Methyl-Endo-Tricyclo [5.2.1.0(2.6)] Decane, 2,4-Dimethylhexan-3-One, Benzene acetic acid,2-phenylethyl ester, Cyclopentacycloheptene, 2,3-Heptanedione, 1,6-Methano[10] annulene, 1-Naphthaleneacetic acid, methyl ester, N,N-Dimethylmethanesulfonamide, Methyl tridecanoate, Cis 9-Octadecanoic acid, Methyl 15-methylheptadecanoate, 9-Octadecenoic acid, Methyl (Z)-octadec-9-enoate, (Z)-octadec-9-enamide, Methyl 2-ethyl-2-methylicosanoate, 1,2,3,4,4a,5,6,7,8,9,10,11,12,12a-tetradecahydrobenzo [10] annulene Caffeic acid, Catechin, Chlorogenic acid, Chrysin, Cinnamic acid, Epicatechin, Ferulic acid, Kaempferol, Luteolin, Lycopene, Naringenin, P-coumaric acid, Phloretic acid, Quercetin, Resveratrol, Rutin, Sinapic acid, Vanillic acid. Lycopene, the main dietary carotenoid in tomato and tomato-based food products and lycopene consumption by humans has been reported to protect against Cancer, Cardiovascular Diseases, Cognitive function and Osteoporosis. Among phenolic compounds present in tomato, Quercetin, Kaempferol, Naringenin, Caffeic Acid and Lutein are the most common. These compounds have significant antioxidant properties and are effective in protecting human body against oxidative stress-related diseases. Keywords: Solanum lycopersicum; Phytochemical Screening; GCMS; FTIR

The current study aimed to conduct phytochemical screening, FTIR, and GCMS analysis in squash (Cucurbita pepo L.,) also known as a yellow and white selected pumpkin. It’s one of the dicotyledonous vegetables consumed in daily diets that imparts high inhibitor properties of inflammation, cancer, and diabetes. Traditionally it is used as an anti-helminthic remedy. The phytochemical characterization can facilitate seeking out the substance with a therapeutic property. The peel, flesh, and seed sample of each pumpkin variety were used as sources and extracted consecutively with ethyl acetate and acetonitrile using the maceration method. Phytochemical screening and quantification were carried out by standard analytical methods. The functional groups of the sample extracts were analyzed using FT-IR methods. Further, phytochemical profiling was carried out utilizing the GCMS technique to identify the therapeutically important chemicals contained in the sample. Phytochemical analysis of ethyl acetate and acetonitrile extracts showed the presence of major components like alkaloids, phenol, carbohydrate, and proteins. The farthest alkaloid, phenol, carbohydrate, and protein varied consequently for different parts like peel, flesh, and seed. The FT-IR analysis of each extract in the peel, flesh, and seed revealed that the ethyl acetate extract had the most functional groups. The major peak was characterized at wavelength 3004.24 to 3421.05 nm which indicates O-H functional group. Further quantification and GC-MS analysis were performed in ethyl acetate extract. Remarkably, GC-MS analysis of yellow and white pumpkin ethyl acetate extracts showed the utmost 6 - 8 compounds within the flesh part. Further, employing these compounds for anti-inflammatory and anti-microbial assays may aid in the discovery of new drugs for therapeutic applications.

Moringa oleifera has been reported to be the store-house of wide range of bioactive compounds. Most commonly used plant part has been the leaves which are reported to be rich in Vitamins, Carotenoids, Polyphenols, Phenolic Acids, Flavonoids Alkaloids, Glucosinolates, Isocyanides, Tannins and Saponins. Moringa leaves are used as Keerai while, green pods are commonly used as vegetable in the traditional preparation of Sambar in South-India. MO is gaining popularity because of its nutrient-rich root, leaves, flowers and fruits, having immense traditional medicinal uses and proved pharmacological properties. Not much of work has been carried out on analysis of bioactive compounds present in the pods. In the present study an attempt has been made to screen and analyze the range of bioactive compounds present in Moringa oleifera seed removed ripened natural dried pods. Phytochemical screening and GCMS analysis revealed the presence of 12 compounds namely - 7-Octadecyne, 2-methyl- (C19H36); 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (C20H40O); 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (C20H40O); 6,9,12,15-Docosatetraenoic acid, me (C23H38O2); Cyclohexanol, 5-methyl-2-(1-methylethyl)- (C10H20O); 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (C20H40O); Palmitic acid vinyl ester (C18H34O2); .gamma.-Tocopherol (C28H48O2); Vitamin E (C29H50O2); Cholesta-7,9(11)-dien-3-ol, 4,4-dim (C29H48O); gamma.-Sitosterol (C29H50O); Stigmasta-5,24(28)-dien-3-ol, (3.beta.,24Z)- (C29H48O). Further, in-silico ADMET analysis is expected to provide in-death physiochemical and biomolecular details of these molecules in order to exploit them for production of novel drugs for the pharma market with wide array of bio medical applications. Keywords: Bioactive Compounds; GCMS; Phytochemical Screening; MOPME; Plant Based Natural Products

Alternaria porri causing purple blotch disease of onion is a destructive phytopathogen which causes severe loss in productivity. The present study aimed to unravel the antagonistic potential and efficacy of volatile organic compounds produced by various Trichoderma spp. against A. porri causing purple blotch disease of onion through Gas chromatography-mass spectrometry (GCMS) analysis. Ten isolates of Trichoderma species were isolated from the rhizospheric soil of healthy onion plants. Upon paired plate technique, the in vitro efficacy of ten Trichoderma isolates were tested against virulent isolate of Alternaria porri isolated from purple blotch disease infected onion plants. The Trichoderma isolate TIM2 showed 76.29 per cent inhibition on mycelial growth of pathogen. The effective Trichoderma isolate was identified as Trichoderma hamatum through the analysis of the rDNA of internal transcribed spacers (ITS) region and it was subjected to GC-MS analysis. The result of GCMS analysis indicated the highest peak area and retention time with major antimicrobial bioactive compounds like Tetradecane, 2,6,10-trimethy (20.327), (1.22) and Dodecane, 2-cyclohexyl (20.079), (2.14), Heptadecane (21.222), (9.50), Octadecane (22.379), (3.58), Eicosane, 9-cyclohexyl (22.578), (1.84), 2-Propenoic acid, pentadecyl ester (23.400), (10.37), 2,6,10,14-tetramethyl (23.567), (10.37), Eicosane (27.311), (2.34), Hexadecanoic acid, methyl ester (27.918), (4.43), n-Hexadecanoic acid (29.156), (3.59) and Tetrapentacontane, 1,54-dibromo (31.906), (3.33). These bioactive compounds identified through GCMS analysis from the crude extracts of Trichoderma hamatum exhibited a stronger antifungal activity against A. porri. Hence the application of T. hamatum for the management of purple blotch disease highly supress growth of the pathogen and reduce the disease incidence.

This study was designed to apply the highly sophisticated biological and chemical characterization techniques–(GC-MS) Gas chromatography-mass spectrometry and FTIR spectroscopy to screen for bioactive phytocompounds present in ethanol and methanol extracts of U. lobata .The leaves of the plant were collected from a farm in Umuode village in Osisioma Ngwa local government area of Abia state, Nigeria. Ethanol and methanol extracts of the leaves were prepared and analyzed using Buck M910 Gas chromatography system with HP-5MS column (30m in length x250µm in diameter x 0.25µm in thickness film). Analysis of functional groups in the phytocompounds were done using Buck scientific M530 USA FTIR.12 vibrational functional groups were revealed by the FTIR screening in the ethanol and methanol extracts of U. lobata leaves. Some of the functional groups were indicative of alcohols, phenols, aromatic compounds, unsaturated hydrocarbons, vinyl ethers, amines, isonitriles and aliphatic compounds. GC-MS analysis of the ethanol leaf extract revealed 41 bioactive compounds with the following being most abundant, 9-octadecenoic acid(16.8%), dodecenoic acid(13.43%),n-hexadecanoic acid(11.73%), octadecanoic acid (9.78%), 1-docosene(9.57%) while the methanol extract revealed 47 bioactive compounds with the following having the highest abundance, n-hexadecanoic acid (26.65%) and (9.11%), dodecanoic acid(6.89%), 1-docosene(6.06%), erucic acid(4.09%).These phytocompounds and many others present in the leaf have been reported to possess multiple therapeutic activities. This therefore explains the use of this leaf in ancient medicine to treat numerous disease conditions. It further implies that the leaf could be exploited for the formulation of therapeutic molecules. Keywords: Urena lobata, GC-MS, FTIR, Spectroscopy, Phytocompounds, Functional group, Phytoconstituents

Global warming has amplified the frequency of temperature extremes, especially in hot dry countries, which could have serious consequences for the natural and built environments. Egypt is one of the hot desert climate regions that are more susceptible to climate change and associated hazards. This study attempted to project the changes in temperature extremes for three Shared Socioeconomic Pathways (SSPs), namely, SSP1-2.6, SSP2-4.5, and SSP5-8.5 and two future periods (early future: 2020-2059 and late future: 2060-2099) by using daily maximum (T

Fusarium oxysporum f. sp. cepae causing basal rot disease of onion is a destructive phytopathogen resulting in 30-50% yield loss and remains as a major constraint in onion productivity. The management of disease through application of fungicide is not feasible and economically viable. Hence, the present study is focused on investigation of effective Trichoderma sp. and identifying the effective volatile organic compounds produced by it against the basal rot pathogen in onion. A total of ten Trichoderma spp. were isolated from rhizospheric soil of healthy onion plants and tested against virulent Fusarium oxysporum f. sp. cepae isolate FCIM1. The Trichoderma isolate (TIM2) showed 77.40% inhibition on mycelial growth of pathogen followed by the isolate (TIV1) with 70.36% inhibition. The molecular identification of effective Trichoderma isolate through the analysis of the rDNA of Internal Transcribed Spacers (ITS) region revealed isolate TIM2 as Trichoderma hamatum. The GC-MS analysis of Trichoderma hamatum unravelled the important volatile organic compounds like Methyl stearate, n-Hexadecanoic, Eicosane, 9-cyclohexy, Heptadecane, Dodecane, 2-cyclohexyl, to 2H-Pyran-2-one, 6-pentyl, 5-Hydroxymethylfurfural, Tetrapentacontane, 1-Dodecanol, 2-Propenoic acid, pentadecyl ester, Benzene, (2-methylbutyl) and 1,2-Dimethyltryptamine with peak area and retention time. These bioactive compounds exert a strong antifungal activity against Fusarium oxysporum f. sp. cepae. The scanning electron micrographs of Fusarium paired with effective Trichoderma (TIM2) showed the swollen hyphae with cell wall damage which is clear evident of antagonistic interaction of volatile compounds produced by Trichoderma hamatum.

To perform the TLC and GCMS analysis of ethanolic extract of seed Momordica charantia. The seeds ofMomardicacharantia was extracted with ethanol and tested for TLC and GCMS by standard procedure as per the guidelines of WHO.The phytochemical and TLC studies showed the presence of alkaloids, cardiac glycosides, phenols, steroids, terpenoids and proteins.The GCMS analysis of seed Momardicacharantiashowed five major compounds were found to be n-hexadeconic acid, 9,12-Octadecadinoic acid,Octadecanoic acid,Hexadecanoic acid-2-hydroxy-1-hydroxy methyl ethyl ester and Gamma-sitosterol.The Therapeutic activity may be due to the major compounds identified by GCMS and further have to be evaluated.Mostly cucurbitaceaeplant possesses the highest nutritive value and also a good source of carbohydrates, proteins, fibers, vitamins, and minerals. Fruits are composed of sufficient of water,protein and lipids respectively.In addition to this MC seeds can represent a good source of lipids, such as polyunsaturated fatty acids and they are among the few foods containing conjugated linolenic acid, being as eleostearic acid. The essential oil, obtained from drought seeds, contains sesquiterpenes, phenylpropanoids and monoterpenes. Other bioactive compounds, such as tocopherols and polyphenols have been reported in general. The pericarp, the aril, the stem and the leaves of the plant are also a good source of phenolic compounds, which can be useful to protect from oxidative damage by acting directly on reactive oxygen species and to induce endogenous defense systems. &nbsp

The frequency of atmospheric blocking has been largely underestimated by general circulation models (GCMs) participating in the Coupled Model Intercomparison Project (CMIP). Errors in the onset, persistence, barotropicity, geographical preference, seasonality, intensity, and moving speed of global blocking were diagnosed in 10 Geophysical Fluid Dynamics Laboratory (GFDL) GCMs for recent CMIP5 and CMIP6 using a detection approach that combines zonal eddies and the reversal of zonal winds. The blocking frequency, similar at 500 and 250 hPa, is underestimated by 50% in the Atlantic–Europe region during December–February but is overestimated by 60% in the Pacific–North America region during that season and by 70% in the southwest Pacific during July–August. These blocking biases at 500 hPa were investigated in the five CMIP6 models that showed improvements over the CMIP5 versions. The Atlantic–Europe underestimate corresponds to lower instantaneous blocking rates, lower persistent blocking rates, and higher persistent stationary ridge rates; the number of blocks with a duration of 4–5 days is only 40%–65% of that in observations. In contrast, the overestimate consists of excessive blocks with a duration longer than 12 days in the Pacific–North America and up to twice as many 4–6-day events in the southwest Pacific. Simulated December–February blocks up to 12 days in the Pacific–North America region tend to be stronger and to move more slowly than those in observations. Diagnostic sensitivity tests indicated that the zonal mean and zonal eddy components of the mean state play a key role, as replacing each with that of observations substantially reduced many of the outstanding biases in these GCMs.

Objective: Andrographis paniculata was an ancient medicinal herb recorded in Ayurvedic system of medicine from long times. The present study is to determine the bioactive phytocomponents present in Andrographis paniculata seed, extraction done using ethanol and Human Liver Cancer (HepG2) cells are used to prove the anticancer activity. Methods: Gas Chromatography-Mass Spectroscopy (GCMS) analysis is used for determining the bioactive constituents of the Andrographis paniculata seed. To prove the in vitro anticancer activity, the MTT assay was done on Human Liver Cancer (HepG2) cells. Results: GCMS analysis of Andrographis paniculata seed extract result determined the presence of 15 active bio components. These various potent bioactive compounds have been proved to possess various pharmacological activities, viz., antimicrobial, antidiabetic, antioxidant, anti-inflammatory and anticancer properties, which were supported by previous findings. The natural compounds with therapeutic effects on traditional medicine have paved the way for evaluating the Andrographis paniculata seeds for cytotoxic activity on HepG2 cell lines. The ethanol extract of Andrographis paniculata seeds was used on HepG2 to evaluate the cytotoxicity; Cisplatin (15 µg/ml) is used as standard with various concentration like 200, 100, 50 and 25 µg/ml were used. The bioactive compounds present in Andrographis paniculata seed extract revealed significant cytotoxicity activity against HepG2 cells with IC50 value of 103.03µg/ml. Conclusion: The cytotoxic effect was found to be concentration-dependent and increased concentration of Andrographis paniculata seed extract showed increased cytotoxicity. The result suggest Andrographis paniculata seed extract have bioactive agents which when treated on hepatocellular carcinoma cells poses excellent anticancer activity.

ABSTRACT This study examined the use of Fast Blue B Salt (FBBS) spray as a semi-confirmed colorimetric test in the urine examination of suspected Methamphetamine (MA) and Methylenedioxymethamphetamine (MDMA) narcotic suspects. The application of spray of Fast Blue B Salt with a concentration of 1000 ppm on urine extract which was spotted on an HPTLC silica gel 60 F254 plate was carried out at room temperature with eluent of Ethyl acetate: Methanol: Ammonia (8.5: 1: 0.5) with solvent of Chloroform: Isopropanol (1: 1) by using CRM MA and CRM MDMA comparison. The presence of MA and MDMA in the suspect's urine was identified by the appearance of a color on the TLC (Thin Layer Chromatography) plate, namely a yellowish orange color for positive MA with an Rf of 0.63 { on trak1 (urine 9416) and trak3 (urine 9577)} and a dark yellowish violet color for MDMA positive with Rf 0.67 { on track 2 (urine 9768A1) and track 4 (urine 9768B)} where the color and Rf of the compound are the same as the comparison color for track 5 (CRM MA) and track 6 (CRM MDMA). The selectivity of the new FBBS colorimetric test method for the color of MA and MDMA compounds on TLC plates was confirmed using HPTLC (High Performance Thin Layer Chromatography) and GCMS (Gas Chromatography Mass Spectroscopy). HPTLC and GCMS confirmation results on TLC plates that were previously Ma positive (urine 9416 and urine 9577) were confirmed to be positive for MA at 215 nm and 260 nm and Rt 3.6 MS m/z (149, 134, 91,58), as well as previously positive for MDMA (urine 9768A1 and urine 9768B) confirmed to be positive for MDMA at λ 215 nm, 240 nm and 280 nm and Rt 6.3 MS m/z (193, 178, 135, 89, 58). The occurrence of specific colors of MA compounds with yellowish orange color and dark violet-yellow MDMA by a new colorimetric test method (Fast Blue B Salt spray) can be tested for selectivity by TLC, HPTLC and GCMS. ABSTRAK Penelitian ini tentang penggunaan spray Fast Blue B Salt (FBBS) sebagai uji kolorimetri semi konfirmasi dalam pemeriksaan urine tersangka pengguna narkotika jenis Metamfetamina (MA) dan Metilendioksimetamfetamina (MDMA). Pengaplikasian spray Fast Blue B Salt konsentrasi 1000 ppm pada ekstrak urine yang ditotolkan pada plat HPTLC silica gel 60 F254 dilakukan pada suhu kamar dengan eluen Etil asetat : Metanol : Amoniak (8,5: 1 : 0,5) dengan pelarut Kloroform: Isopropanol (1 : 1) dengan menggunakan pembanding CRM MAdan CRM MDMA. Adanya MA dan MDMA didalam urine tersangka diidentifikasi dengan munculnya warna pada plat TLC (Thin Layer Chromatography) yaitu warna orange kekuningan untuk positif MA dengan Rf 0,63 { pada trak1 (urine 9416) dan trak3 (urine 9577)} dan warna violet tua kekuningan untuk positif MDMA dengan Rf 0,67 { pada trak 2 (urine 9768A1) dan trak 4 ( urine 9768B)} dimana warna dan Rf senyawa tersebut sama dengan warna pembanding trak 5 (CRM MA) dan trak 6 (CRM MDMA). Keselektivan metode baru uji kolorimetri FBBS terhadap warna senyawa MA dan MDMA pada plat TLC diuji konfirmasi dengan menggunakan HPTLC (High Performance Thin Layer Chromatography) dan GCMS (Gas Chromatography Mass Specrtroscopy). Hasil konfirmasi HPTLC dan GCMS terhadap plat TLC yang sebelumnya positif Ma (urine 9416 dan urine 9577) terkonfirmasi benar positif MA pada λ 215 nm dan 260 nm dan Rt 3,6 MS m/z ( 149, 134, 91,58), begitupun sebelumnya positif MDMA (urine 9768A1 dan urine 9768B) terkonfirmasi benar positif MDMA pada λ 215 nm, 240 nm dan 280 nm dan Rt 6,3 MS m/z ( 193, 178, 135, 89, 58 ). Munculnya warna spesifik senyawa MA warna orange kekuningan dan MDMA warna violet tua kekuningan oleh metode baru uji kolorimetri (spray Fast Blue B Salt) dapat diuji keselektivannya dengan TLC, HPTLC dan GCMS.

Momordica charantia, commonly called bitter melon, is a plant belonging to Cucurbitaceae family known for centuries for its nutritional properties. This plant have been used as folklore medicine for the management of hypertensions, cold, cough, and diabetes. The aim of the present study was to characterize the chemical constituents of ripe M. charantia from Lombok, Indonesia by Gas Chromatography Mass Spectrometry (GCMS). The method that used is maseration with ethanol as solvent. The ethanol extract of ripe M. charantia was characterized by gas chromatography-flame ionization detection and gas chromatography coupled with mass spectrometry analyses. The chemical constituent of ripe M. charantia that followed by GCMS were 2,4-Pentanediol (23,61 %), Eucalyptol (1,8-Cineole) (20,41 %), Trans (Beta)-Caryopyllene (10,07 %), 3-Decyn-2-ol (9.54 %), Epiglobulol (7.82 %), Methyleugenol (7.15%), Beta-Selinene (5.80 %), Alpha-Selinene (5.43 %), and Alpha-Humulene (5.29 %). This is the first report on chemical screening of ethanol extract of ripe M. charantia and GC-MS analysis shows the presence of compounds which have medicinal properties.

In the Eastern Mediterranean countries, groundwater contained in coastal aquifers is the predominant water source for supplying water. The Mediterranean Gaza coastal aquifer in Palestine is showing alarming signs of depletion due to climate change and human-caused influences that substantially impact the hydraulic performance of the Gaza coastal aquifer. The climate statistical modeling and the downscaling of the ensemble global climate model under the representative concentration pathway (RCP) scenarios of RCP 2.60, 4.50, and 8.50 refer to a future decreasing trend in the precipitation and an increasing trend in the temperature. The stochastic model refers to an average decrease of −5.2% in the rainfall every 20 years that coincides with the RCP scenarios that show a decrease in precipitation between 0 and −5%. The rate of temperature increase over the next 20 years is defined at +1 °C, which closely matches the RCP results. As a result of human fast-paced activities, groundwater consumption is expected to rise by nearly 55% by the end of 2040, to around 193 million cubic meters, with a substantial withdrawal trend in southern provinces of the Gaza Strip. Consequently, the aquifer model predicts that subsurface water levels might fall at a rate of −2.50% per year, reaching a level of around −27.77 m below the mean sea level (MSL) by 2040. Alternative water supplies, such as desalinated seawater and treated wastewater, with annual maximum volumes of 110 and 16.5 million cubic meters, respectively, are expected to perfectly recharge groundwater resources of the Gaza coastal aquifer at an annual rate of about +3.65% to meet a groundwater table level of around −5 m below the MSL by 2040.

Extreme weather events are more detrimental to human culture and ecosystems than typical weather patterns. A multimodel ensemble (MME) of the top-performing global climate models (GCMs) to simulate 11 precipitation extremes was selected using a hybrid method to project their changes in Pakistan. It also compared the benefits of using all GCMs compared to using only selected GCMs when projecting precipitation extremes for two future periods (2020–2059) and (2060–2099) for four shared socioeconomic pathways (SSPs), SSP1-2.6, SSP2-4.5, SSP3-7.0 and SSP5-8.5. Results showed that EC-Earth3-Veg, MRI-ESM2-0 and NorESM2-MM performed best among GCMs in simulating historical and projecting precipitation extremes. Compared to the MME of all GCMs, the uncertainty in future projections of all precipitation indices using the selected GCMs was significantly smaller. The MME median of the selected GCMs showed increased precipitation extremes over most of Pakistan. The greater increases were in one-day maximum precipitation by 6–12 mm, five-day maximum precipitation by 12–20 mm, total precipitation by 40–50 mm, 95th percentile precipitation events by greater than 30 mm, 99th percentile precipitation events by more than 9 mm, days when precipitation ≥ 4 mm by 0–4 days, days when precipitation ≥ 10 mm by 2–6 days, days when precipitation ≥ 20 mm by 1–3 days, and precipitation intensity by 1 mm/day, consecutive wet days by one day, consecutive dry days by 0–4 days in the northern high elevated areas for SSP5-8.5 in the late future. These results emphasize the greater influence of climate change on precipitation extremes in the northern, high-elevation areas, which provide the majority of the country's water. This emphasizes the necessity to adopt suitable climate change mitigation strategies for sustainable development, particularly in the country's northern regions.

Data Assimilation in paleoclimatology (PaleoDA) is a method that has been used in several climate reconstructions for the last millennium. By fusing information from both climate proxies and general circulation models (GCMs), PaleoDA provides statistical estimates of climate fields that are dynamically consistent. However, existing reconstructions mostly rely on calibrated tree ring data and assimilate proxy records on a single, annual time scale. Ice cores and speleothems, which record past variations in the oxygen isotope ratio of precipitation, often have a lower and irregular time resolution, but reliably record climate variations on decadal to centennial time scales. Here, we implemented a computationally efficient DA algorithm that enables the assimilation of proxy records on multiple timescales. The algorithm has been applied to speleothem and ice core records from the SISALv2 and Iso2k database and five isotope-enabled GCMs. Reconstructions of global mean temperature changes during the last millennium compare well in both amplitude and uncertainty to recent studies. The potential of incorporating speleothems is shown with a reconstruction of hydroclimatic changes in tropical South America, where speleothems represent the most abundant type of hydroclimate archive. The experiments performed suggest an increased reconstructed decadal to centennial variability by using proxy records on multiple timescales. Making use of different climate models shows the influence of model biases on the reconstructions. Future PaleoDA reconstructions could be improved from more proxy records and the multiple time scale approach to provide a globally complete picture of past climate changes.

Global climate models (GCMs) represent invaluable instrument for various purposes, most prominently studying climate system dynamics, evolution of past climates and climate change projections. The newest set of GCM simulations has been produced under CMIP6 initiative coordinated by the the World Climate Research Programme’s (WCRP) Working Group on Coupled Modelling. Due to their coarse spatial resolution, some kind of downscaling is necessary for applications of GCM outputs on regional and local scales, with dynamical downscaling using regional climate models (RCMs) being a common solution of this issue. Clearly, the outputs of RCMs are influenced by the boundary conditions provided at the lateral boundaries of the integration domain. The magnitude of this influence is subject of ongoing research and depends on various aspects including the geographical region, temporal scale, climatic variable etc. Nevertheless, previous studies proved that an analysis of boundary conditions is needed for for proper RCM evaluation. Especially with regard to potential error propagation. In present study we evaluate the simulation of CNRM-ESM2-1, one of the CMIP6 GCMs, and compare it to other CMIP6 ensemble members. The CNRM-ESM2-1 is being used as driving model for convective-permitting simulation of Aladin-CLIMATE RCM within Czech national project PERUN aimed at creation of updated climate change scenarios for the Czech Republic. We concentrate on historical GCM simulations in the period of 1990-2014, and use reanalysis ERA5 as the reference. The analysis is conducted over the boundaries of Aladin-CLIMATE/CZ intergration domain, which covers Central Europe. The deviations of the driving GCM (CNRM-ESM2-1) from reanalysis are compared to other CMIP6 GCMs. We also evaluate uncertainty arising from natural climate variability using perturbed initial conditions ensemble of CNRM-ESM2-1.

Cloud fraction significantly affects the short- and long-wave radiation. Its realistic representation in general circulation models (GCMs) still poses great challenges in modeling the atmosphere. Here, we present a neural network-based diagnostic scheme that uses the grid-mean temperature, pressure, liquid and ice water mixing ratios, and relative humidity to simulate the sub-grid cloud fraction. The scheme, trained using CloudSat data with explicit consideration of grid sizes, realistically simulates the observed cloud fraction with a correlation coefficient (r) > 0.9 for liquid-, mixed-, and ice-phase clouds. The scheme also captures the observed non-monotonic relationship between cloud fraction and relative humidity and is computationally efficient, and robust for GCMs with a variety of horizontal and vertical resolutions. For illustrative purposes, we conducted comparative analyses of the 2006-2019 climatological-mean cloud fractions among CloudSat, and simulations from the new scheme and the Xu-Randall scheme (optimized the same way as the new scheme). The network-based scheme improves not only the spatial distribution of the total cloud fraction but also the cloud vertical structure (r > 0.99). For example, the biases of too-many high-level clouds over the tropics and too-many low-level clouds over regions around 60{\deg}S and 60{\deg}N in the Xu-Randall scheme are significantly reduced. These improvements are also found to be insensitive to the spatio-temporal variability of large-scale meteorology conditions, implying that the scheme can be used in different climate regimes.

In Titan, the two major gases nitrogen (N2) and methane (CH4) are ionized and/or photolyzed at high altitudes by the sunlight and the energetic particles from Saturn's magnetosphere, resulting in rich atmospheric chemistry and a wide variety of carbon and nitrogen-bearing atmospheric compounds. In the present work, we focus on studying the vertical profiles of trace species in the lower atmosphere to obtain a better insight into the atmospheric processes taking place on Titan. To do so, we reanalyzed the data from the Gas Chromatograph Mass Spectrometer (GCMS) on the Huygens probe which executed its mission on 14th January 2005. The GCMS instrument sampled for nearly three and a half hours from a height of 146 km. It recorded data for two and a half hours in the atmosphere of Titan, then landed on the surface and kept on recording for another hour, after which the signal was lost. We analyzed the measurements made by direct sampling of the atmosphere. These mass spectra obtained at different altitudes and pressure levels have been recalibrated to account for deadtime and saturation corrections to the measurements and considered ion cross-section and transfer cross-section measurements from Cassini-Ion and Neutral Mass Spectrometer calibrations. We then analyzed the corrected mass spectra using Monte-Carlo deconvolution simulations. The simulations allow us to vary the peak intensities of fragmentation patterns of known species, which usually bears uncertainties on this kind of data, and then use a least-square fitting to deconvolve the mixed signals. This is the first time mixing ratios of high-altitude trace gases could be quantified using this GCMS data. Currently, we are working with 10 species and developing their vertical profiles in the atmosphere. In the future, we plan to extend this study to develop a sub-surface model of Titan which will help us understand the outgassing of methane that was observed by the probe upon its touchdown on the surface.

Over centuries, Moringa oleifera has been used as an integral part of Ayurveda, Siddha and Unani systems of medicine, besides this miracle tree has a wide range of nutritional and bioactive compounds, including proteins, essential amino acids, carbohydrates, lipids, fiber, vitamins, minerals, phenolic compounds, phytosterols and others. The miracle tree is endowed with a wide range of pharmacological properties, including anti-diabetic, anti-inflammatory, anti-carcinogenic, antioxidant, cardioprotective, antimicrobial and hepatoprotective activities. However, deeper dimensions of authentic data on plant based natural products in relation to drug discovery, pharmacokinetics properties of biomolecules is far lacking. ADMET prospecting is eventually expected to contribute to success of MO based lead candidates in drug design, development program besides saving time and money. This study is the first of its kind reporting summative ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicology) properties of all bioactive compounds Moringa oleifera methanolic pod extract (MOMPE) as a potential source of natural drug lead using Swiss ADME. A total of 12 compounds namely - 7-Octadecyne, 2-methyl- (C19H36); 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (C20H40O); 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (C20H40O); 6,9,12,15-Docosatetraenoic acid, me (C23H38O2); Cyclohexanol, 5-methyl-2-(1-methylethyl)- (C10H20O); 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (C20H40O); Palmitic acid vinyl ester (C18H34O2); .γ.-Tocopherol (C28H48O2); Vitamin E (C29H50O2); Cholesta-7,9(11)-dien-3-ol, 4,4-dim (C29H48O); γ.-Sitosterol (C29H50O); Stigmasta-5,24(28)-dien-3-ol, (3.β.,24Z)- (C29H48O). Most of the MOPBNPs are non-substrate for both P-gp (P-glycoprotein) and CYP (Cytochrome P-450 isoenzymes). All the compounds were evaluated for properties viz., GI absorption, BBB permeant, Pgp substrate, CYP1A2, CYP2C19, CYP2C9, CYP2D6, CYP3A4 inhibitors, Lipinski, Ghose, Veber, Egan, Muegge, rule and Bioavailability Score to provide baseline information on PBNPs in MONRD pod. Keywords: PBNPs; NGDDT; ADMET; Moringa oleifera; Secondary Metabolites MONRD pod

Background and Objective: Inflammation is a common disease which can induce many diseases. There are unique advantages of Traditional Chinese Medicine (TCM) to anti-inflammation. Tieguanyin (TGY) is a well-known beverage; the quality is determined by aroma, taste, liquor color, and shape. The volatile compounds produce the flavor of tea, which can be lost with the increase of storage time. TGY has an excellent antiinflammatory effect; its volatile compounds also have an anti-inflammatory impact that is unclear. This study aimed to identify volatile compounds and anti-inflammatory mechanisms within the validity period (TGY1) and the out-of-date (TGY2). Methods: The volatile compounds of TGY1 and TGY2 were analyzed with headspace solid-phase microextraction (HS-PME) and identified by Gas chromatography-mass spectrometry (GC-MS). The percentage of volatile compounds was calculated by the peak area normalization method. The compounds of the targets were obtained from the Traditional Chinese Medicine Systems Pharmacology (TCMSP), PubChem Database, and Swiss Target Prediction database. Next, the disease potential targets were screened by the GeneCards database, Online Mendelian Inheritance in Man (OMM) database, and Therapeutic Target Database (TTD). Furthermore, core targets were screened by the Search Tool for the Retrieval of Inter-acting Genes/Proteins (STRING) database. Then, Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of core targets was performed by the ClueGo plugin Cytoscape 3.7.1 software. At last, Autodock vina software performed molecular docking between the main compounds and core targets. Results: Significant differences in volatile compounds and percentage contents in TGY were observed; the 61 volatile compounds in TGY1 and 57 volatile compounds in TGY2 were identified. After excluding the unidentified compounds, a total of 47 volatile compounds were obtained from TGY1 and TGY2. With the use of network pharmacology, 34 core targets and 23 signaling pathways from TGY1, 28 core targets, and 19 signaling pathways from TGY2 were screened. The main common core targets of TGY1 and TGY2 contained MAPK3, TNF, MAPK1, SRC, etc., while the main different core targets included PTGS2, CAT, etc. A total of 12 biological processes are shared by TGY1 and TGY2, among which the cellular response to oxidative stress is the primary biological process. The different biological processes of TGY1 and TGY2 include cellular response to lipopolysaccharide, androgen receptor signaling pathway, etc. There were 14 common signaling pathways in TGY1 and TGY2, among which the thyroid hormone signaling pathway is the main common signaling pathway. The differential signaling pathways in TGY1 and TGY2 included the erbB signaling pathway, Chagas disease, etc. Molecular docking results showed that the ordinand and differential volatile compounds of TGY1 and TGY2 had different binding forces with the core targets. Conclusion: The GC-MS experiment showed significant differences in volatile compounds and percentage contents in TGY1 and TGY2. Network pharmacology indicated that they have anti-inflammatory effects. Besides, they were different in core targets, biological processes, and signaling pathways but shared similar anti-inflammatory mechanisms. Molecular docking results showed that the binding force of the TGY1 compounds to the core target is greater than that of the TGY2. Therefore, expired TGY affects volatile compounds, resulting in differences in the anti-inflammatory mechanism. The study provided a theoretical framework for further development and application of used medicinal and edible species. In addition, the application of expired TGY under safe conditions can also have anti-inflammatory effects. These results shed new light on the rational use of resources.

The projected temperature, rainfall and solar radiation derived from four General Circulation Models namely CSIRO-Mk3-6-0, FIO-ESM, IPSL-CM5A-MR and Ensemble model under four RCP (Representative Concentration Pathways) scenarios were analyzed on annual basis for four agro-climatic zones of Punjab. The inherent bias in the simulated data was corrected by the difference method at monthly scale for maximum temperature, rainfall and at daily scale for solar radiation. In general, the temperatures are expected to increase linearly in future while the solar radiation and rainfall would decrease under the four RCP scenarios. Amongst the four models, the maximum temperature is predicted to increase from the baseline during mid/end century by 0.5 to 1.2/1.6 to 2.6 °C and minimum temperature is predicted to increase from the baseline during mid/end century by 1.1 to 2.6/2.7 to 4.1 °C by CSIRO-Mk3-6-0, respectively followed by IPSL-CM5A-MR model in decreasing order. The solar radiation predicted by four models is predicted to decrease from baseline during mid/end century by 0.5 to 1.1/0.3 to 1.2 W/m2 and rainfall during mid/end century by 96 to 558/59 to 530 mm by CSIRO-Mk3-6-0, respectively followed by FIO-ESM model in decreasing order. The highest/lowest maximum temperatures were predicted by four models under four RCP scenarios were 32.9 °C (Bathinda)/30.5 °C (Ludhiana), highest/lowest minimum temperature were 20.8 °C (Patiala)/17.9 °C (Amritsar), highest/lowest solar radiation 15.9 W/m2 (Amritsar and Bathinda)/14.9 W/m2 (Amritsar) and highest/lowest rainfall 659 mm (Amritsar)/346 mm (Abohar), respectively.

Water hyacinth is a weed in Rawa Pening because of its rapid growth. A handling effort is a very crucial thing and must be done immediately. This study aims to characterize organic compounds in liquid smoke from dried water hyacinth. ­­­­Characterization of organic compounds from dried water hyacinth was carried out on water hyacinth liquid smoke which was pyrolyzed at 200°C and 600°C. Pyrolysis times were run at 2 hours and 6 hours. Liquid smoke from dried water hyacinth was distilled before testing its chemical composition using GCMS brand Shimadzu type QP 2010S. The results of liquid smoke characterization are used to decide that liquid smoke can be utilized as a food preservative. The test results report that the liquid smoke produced had an acid percentage ranging from 29.63% to 37.23%, phenol from 1.04 to 6.11%, and the remaining carbonyl compounds from 55.99% to 68.90%. The highest component value was obtained at 600°C pyrolysis and 6 hours. The conclusion is that liquid smoke from dried water hyacinth can be used as a food preservative because it contains acid, phenol, and carbonyl and is free of Benzo (a) pyrene, which can cause cancer. So that the requirement for liquid smoke as a preservative can be achieved, whereas acid is a food preservative. This study generates the acquisition of natural food preservatives to utilize weeds. The research helps us utilize weeds to resist the Rawa Pening environment and manufacture food preservatives.

Marine algae are one of the largest sources for biogenic compounds. They produce a wide variety of chemically active metabolites. They are found to possess significant antioxidant, and phytochemical activities. Macroalgae provide a great variety of metabolites and natural bioactive compounds with antimicrobial activity, such as polysaccharides, polyunsaturated fatty acids, phlorotannins other phenolic compounds and carotenoids. In the present study the phytochemical, amylase activity, GCMS and Antioxidant assays such as ABTS assay and DPPH assays were carried out for Saccharina japonica using two different solvents, petroleum ether and ethyl acetate. For antioxidant activity, DPPH radical assay and ABTS radical cation scavenging assay were carried out.

Seeds of cumin (Cuminum cyminum L.) are widely used as a spice for their distinctive aroma. C. cyminum have been used in traditional medicine to treat a variety of diseases. Literature presents ample evidence for biomedical activities of cumin which is attributed to its bioactive secondary metabolites - terpenes, phenols, and flavonoids. Besides, health effects of cumin seeds have been experimentally validated through phytochemical analysis depicting the presence of a wide array of bioactive secondary metabolites (BASMs) viz., alkaloid, coumarin, anthraquinone, flavonoid, glycoside, protein, resin, saponin, tannin and steroid. Pharmacological studies indicate that BASMs in seeds of C. cyminum exert antimicrobial, insecticidal, anti-inflammatory, analgesic, antioxidant, anticancer, antidiabetic, anti-platelet-aggregation, hypotensive, bronchodilatory, immunological, contraceptive, anti-amyloidogenic, anti-osteoporotic, aldose reductase, α-glucosidase and tyrosinase inhibitory effects. In the present study phytochemical screening, GCMS, FTIR profile of bioactive natural products from C. cyminum has been envisaged. Phytochemical screening revealed the presence of alkaloids, anthraquinones, carbohydrates, coumarins, flavonoids, glycosides, proteins, quinones, saponins, steroids, tannins and terpenoids. GC-MS analysis revealed the presence of 21 compounds, of which Cuminaldehyde was prominent. FTIR analysis showed the presence of a strong peak value for 15 compounds and medium peak value for 6 compounds. Many of the compounds in the list could be ADMET bioprospected for biomedical applications as natural drug leads. Keywords: Cuminum cyminum; Cuminaldehyde; Bioactive Natural Products; GCMS; FTIR

Plant Based Natural Products (PBNPs) have been subject of interest since ancient time due to their use in food, industrial and biomedical applications. Research attention has further augmented to explore their phytochemical composition, properties, and potential application in the post-COVID era. In the present study phytochemical screening has been carried out with Methanolic Leaf Extracts of Moringa oleifera (MLEMO) followed by Gas Chromatography-Mass Spectrometry (GCMS) analysis. Phytochemical analysis of MLEMO revealed the presence of Alkaloids, Carbohydrates, Coumarins, Flavonoids, Glycosides, Phenol, Proteins, Quinones, Saponins, Steroids, Tannins and Terpenoids. Further, GCMS analysis revealed the presence of 41 compounds of which Dihydroxyacetone; Monomethyl malonate; 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl; 1,3-Propanediol, 2-ethyl-2-(hydroxymethyl); Propanoic acid, 2-methyl-, octyl ester; 3-Deoxy-d-mannoic lactone; Sorbitol; Inositol; Cyclohexanemethanol, alpha-methyl-4-(1-methylethyl), Hexadecanoic acid, Methyl palmitate; n-Hexadecanoic acid (Palmitic acid); 9-Octadecenoic acid, methyl ester; Phytol; 9,12,15-Octadecatrienoic acid; Octadecanoic acid; 9-Octadecenamide were prominent. Most of the compounds in the list are bioactive and possess medicinal properties that are expected to serve as a baseline lead for the development of therapeutic agents. Keywords: Phytochemical screening; GCMS; Bioactive Natural Products; Moringa oleifera; MLEMO; Biomedical application