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Bioinformatics is a noteworthy in appreciative the composite mechanisms of the cell and it is very beneficial for biomedical investigators to examination the clinical samples. Bioinformatics includes the usage of methods comprising useful mathematics, computer science, informatics, artificial intelligence (AI), statistics, chemistry, and biochemistry to resolve biological difficulties frequently on the molecular level. Bioinformatics tools provide significant benefits for nutrition discovery agendas such as cost-saving, time-to-market, and in-sight. The practical feature of bioinformatics is to recognize the code of life that is to decode the evidence that exists in the nucleotide sequence. Chemo-informatics is the application of computer and informational methods to challenge chemical problems, which highlight the manipulation of chemical structural information. Bioinformatics having numerous applications such as molecular medicine, personalized medicine, preventive medicine, gene therapy, etc. Several software packages are significant and free, and secure by well-known organizations. These consist of databanks like ChEMBL and SwissSidechain, software tools like 3D visualization, UCSF Chimera, and provides platform for software designers fascinated in structural biology, SwissSimilarity for virtual screening, Swiss-Bioisostere for ligand design, SwissTarget.

In recent years, herbal drugs have flourished as the method of rehabilitation of choice in numerous parts of the world and most of populations consider them as alternative conventional medicaments. However, most of the herbal drugs show poor solubility and bioavailability, which ultimately leads to diminished efficacy and frequency of administration. Consequently, self-emulsifying drug delivery system has been highlighted for poor water-soluble herbal medicaments. Self-emulsifying drug delivery system is an isotropic mixture of phytoconstituents, oil, surfactant, and cosurfactant, and subsequent to oral administration spontaneously endures self-emulsification and forms without emulsion as soon as it comes in contact gastric fluid. Most of the researchers and industrialists are attracted towards this novel and emerging technology as it offers various advantages. The chapter focuses on the mechanism of self-emulsifying, benefits of herbal SEDDS, factors affecting herbal SEDDS with formulation and characterisation of it.

A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of tizanidine HCl and valdecoxib in tablet formulation. The separation was achieved by Luna C 18 column and acetonitrile: phosphate buffer pH 3.5 (50:50 v/v) as eluent, at a flow rate of 0.5 ml/min. Detection was carried out at 227 nm. Etoricoxib was used as an internal standard. The retention time of tizanidine and valdecoxib was found to be 4.43 and 16.60 min, respectively. The method was validated for linearity, accuracy and precision. Linearity for tizanidine and valdecoxib were in the range 0.4-2.0 µg/ml and 4-20 µg/ml, respectively. The developed method was found to be accurate, precise and selective for simultaneous estimation of tizanidine and valdecoxib in tablets.

A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of paracetamol and valdecoxib in tablet formulation. The separation was achieved by Luna C 18 column and methanol: phosphate buffer pH 3.5 (60:40 v/v) as eluent, at a flow rate of 1.0 ml/min. Detection was carried out at 242 nm. Etoricoxib was used as an internal standard. The retention time of PAR and VAL was found to be 3.01 and 8.51 min, respectively. The method was validated for linearity, accuracy and precision. Linearity for paracetamol and valdecoxib were in the range 25-150 µg/ml and 1-6 µg/ml, respectively. The developed method was found to be accurate, precise, and selective for simultaneous estimation of paracetamol and valdecoxib in tablets.