1. In-vitro test system for the evaluation of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) inhibitors based on a single HPLC run with UV detection using bovine aortic coronary endothelial cells (BAECs)
- Author
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G. Dannhardt and H. Ulbrich
- Subjects
Blotting, Western ,Immunology ,Drug Evaluation, Preclinical ,Aorta, Thoracic ,In Vitro Techniques ,High-performance liquid chromatography ,Lipoxygenase ,Diclofenac ,medicine ,Animals ,Cyclooxygenase Inhibitors ,Lipoxygenase Inhibitors ,IC50 ,Chromatography, High Pressure Liquid ,Pharmacology ,Cyclooxygenase 2 Inhibitors ,biology ,Chemistry ,Molecular biology ,Isoenzymes ,Kinetics ,Meloxicam ,Biochemistry ,Cyclooxygenase 2 ,Prostaglandin-Endoperoxide Synthases ,Polyclonal antibodies ,Cyclooxygenase 1 ,biology.protein ,Tetradecanoylphorbol Acetate ,Aceclofenac ,Cattle ,Spectrophotometry, Ultraviolet ,Endothelium, Vascular ,Cyclooxygenase ,medicine.drug - Abstract
Objective and Design: The aim of this study was to develop a new, whole-cell test system which is easy to handle and requires a standard equipment for the parallel screening of COX-1 and COX-2 inhibitors.¶Materials: Bovine aortic endothelial cells (BAECs).¶Treatment and methods: Unstimulated bovine aortic coronary endothelial cells (BAECs) were used as a source of COX-1 and BAECs pretreated with ASA (100 μM) and activated with phorbol myristate acetate (PMA) were used as a source of COX-2. The time- and concentration-dependent induction of COX-2 expression in the BAECs was evaluated by a kinetic profile (HPLC analysis) and detected by Western-Blot analysis using polyclonal antibodies against COX-1 and COX-2.¶Results: In BAECs, diclofenac and meloxicam showed balanced inhibition of COX-1 (IC50: 0.01/0.4 μM) and COX-2 (IC50: 0.03/0.6 μM). Indomethacin inhibited COX-1 more potently than COX-2 (IC50: 0.008/0.04 μM). Aceclofenac inhibited COX-2 more potently than COX-1 (IC50: 3.0/7.3 μM). DFU and Cl-SC57666 [16] inhibited COX-2 (IC50: 0.04/0.001 μM) highly selectively but did not inhibit COX-1 (IC50: >100 μM).¶Conclusions: In summary an assay has been developed, for the determination of IC50-values for inhibitors of COX-1/2 on cells of the same origin, in line with values in the literature. Moreover, new insights have been gained into the relationship of COX-1/2 and lipoxygenase pathways in BAECs by detecting 15- and 12-HETE: Inhibition of COX-1 by the NSAIDs mostly resulted in an enhancement of 15-HETE and 12-HETE release. In contrast inhibition of COX-2 decreased 15-HETE release.¶ more...
- Published
- 2001
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