25 results on '"Elena Porumb-Andrese"'
Search Results
2. Skin Dialogues in Atopic Dermatitis
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Elena Porumb-Andrese, Claudia Florida Costea, Andrei Cucu, Gabriela Rusu-Zota, Daciana Elena Braisteanu, Vlad Porumb, Mihaela Monica Scutariu, Alexandra Maria Dorobanțu, and Ramona Gabriela Ursu
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Clinical Biochemistry - Abstract
Atopic dermatitis (AD) is a chronic skin disorder associated with significant quality-of-life impairment and increased risk for allergic and non-allergic comorbidities. The aim of this review is to elucidate the connection between AD and most common comorbidities, as this requires a holistic and multidisciplinary approach. Advances in understanding these associations could lead to the development of highly effective and targeted treatments. more...
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- 2022
Catalog
3. Differences and Similarities in Epidemiology and Risk Factors for Cutaneous and Uveal Melanoma
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Daciana Elena Brănișteanu, Elena Porumb-Andrese, Alexandra Stărică, Anca Catalina Munteanu, Mihaela Paula Toader, Mihail Zemba, Vlad Porumb, Mihai Cozmin, Andreea Dana Moraru, Alin Codruț Nicolescu, and Daniel Constantin Brănișteanu more...
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General Medicine - Abstract
Both cutaneous melanoma (CM) and uveal melanoma (UM) represent important causes of morbidity and mortality. In this review, we evaluate the available knowledge on the differences and similarities between cutaneous melanoma and uveal melanoma, focusing on the epidemiological aspects and risk factors. Uveal melanoma is a rare condition but is the most prevalent primary intra-ocular malignant tumor in adults. Cutaneous melanoma, on the other hand, is significantly more common. While the frequency of cutaneous melanoma has increased in the last decades worldwide, the incidence of uveal melanoma has remained stable. Although both tumors arise from melanocytes, they are very distinct entities biologically, with complex and varied etiologies. Both conditions are encountered more frequently by individuals with a fair phenotype. ultraviolet-radiation is an important, well-documented risk factor for the development of CM, but has shown not to be of specific risk in UM. Although cutaneous and ocular melanomas seem to be inherited independently, there are reported cases of concomitant primary tumors in the same patient. more...
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- 2023
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4. The Influence of Oncogenic Viruses in Renal Carcinogenesis: Pros and Cons
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Bianca Manole, Costin Damian, Simona-Eliza Giusca, Irina Draga Caruntu, Elena Porumb-Andrese, Catalina Lunca, Olivia Simona Dorneanu, Luminita Smaranda Iancu, and Ramona Gabriela Ursu
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Microbiology (medical) ,Infectious Diseases ,General Immunology and Microbiology ,Immunology and Allergy ,Molecular Biology - Abstract
Viral infections are major contributors to the global cancer burden. Recent advances have revealed that known oncogenic viruses promote carcinogenesis through shared host cell targets and pathways. The aim of this review is to point out the connection between several oncogenic viruses from the Polyomaviridae, Herpesviridae and Flaviviridae families and renal carcinogenesis, highlighting their involvement in the carcinogenic mechanism. We performed a systematic search of the PubMed and EMBASE databases, which was carried out for all the published studies on RCC in the last 10 years, using the following search algorithm: renal cell carcinoma (RCC) and urothelial carcinoma, and oncogenic viruses (BKPyV, EBV, HCV, HPV and Kaposi Sarcoma Virus), RCC and biomarkers, immunohistochemistry (IHC). Our analysis included studies that were published in English from the 1st of January 2012 to the 1st of May 2022 and that described and analyzed the assays used for the detection of oncogenic viruses in RCC and urothelial carcinoma. The virus most frequently associated with RCC was BKPyV. This review of the literature will help to understand the pathogenic mechanism of the main type of renal malignancy and whether the viral etiology can be confirmed, at a minimum, as a co-factor. In consequence, these data can contribute to the development of new therapeutic strategies. A virus-induced tumor could be efficiently prevented by vaccination or treatment with oncolytic viral therapy and/or by targeted therapy. more...
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- 2022
5. The impact of COVID-19 infection of patients with autoimmune blistering diseases of the skin
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Dumitrița-Lenuța Nichitean, Elena Porumb-Andrese, Ioana Alina Grajdeanu, Ioana Adriana Popescu, Laura Stătescu, Laura Gheucă Solovăstru, and Dan Vâță
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medicine.medical_specialty ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Medicine ,General Materials Science ,business ,Dermatology - Published
- 2020
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6. 'Update on pathophysiology of senile pruritus and its impact on quality of life '
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Ioana Adriana Popescu, Dan Vâță, Elena Porumb-Andrese, Laura Gheucă Solovăstru, Diana Aniculaesa, and Alina Ioana Halip
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medicine.medical_specialty ,Quality of life (healthcare) ,business.industry ,medicine ,General Materials Science ,Intensive care medicine ,business ,medicine.disease ,Pathophysiology ,Senile pruritus - Published
- 2020
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7. Inflammatory marker alteration in response to systemic therapies in psoriasis
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Elena Porumb-Andrese, Alina Ioana Grăjdeanu, Adriana Ionela Pătrașcu, Laura Gheucă Solovăstru, Cristina Grechin, and Dan Vâță
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0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,medicine.disease_cause ,Fibrinogen ,Gastroenterology ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,immune dysregulation ,Internal medicine ,Psoriasis ,medicine ,medicine.diagnostic_test ,business.industry ,Acute-phase protein ,Cancer ,General Medicine ,Articles ,psoriasis ,Immune dysregulation ,medicine.disease ,inflammatory markers ,030104 developmental biology ,biological therapy ,030220 oncology & carcinogenesis ,Erythrocyte sedimentation rate ,Methotrexate ,business ,genetic predisposition ,medicine.drug - Abstract
Substantial research has focused on the presence of biomarkers involved in both the pathogenesis of psoriasis and its comorbidities. The identification of these biomarkers has a crucial role in establishing the diagnosis and prognosis, in understanding the physiopathological mechanism and in determining the therapeutic response. The aim of this study was to emphasize the alteration in inflammatory markers in response to systemic therapies in psoriasis. Evolution of inflammatory marker alteration was studied in 194 patients with psoriasis, aged between 7 and 87 years. Two groups were set up: the first comprised of patients treated with methotrexate (n=51), while the second comprised patients treated with biological therapy (n=143). Each group was evaluated for blood values of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and fibrinogen before and after treatment, the fluctuation of these values according to the treatment, the interrelation between inflammatory markers and inflammatory activity of the disease and the evolution of the disease after treatment. In group I, 46 out of 51 patients had elevated levels of acute phase reactants before treatment. After treatment with methotrexate 7.5 mg/week, 12 out of 46 patients had elevated blood levels of ESR and 18 out of 46 patients of CRP and fibrinogen. Before treatment with biological therapy, 138 patients out of 143 presented abnormal high range for acute phase reactants. After treatment with biological therapy, 18 patients out of 138 had elevated blood levels of ESR and 37 patients out of 138 had elevated CRP and fibrinogen. A favorable evolution was noted in 98 patients out of 138. It was concluded that the systemic treatment with both methotrexate and biological therapy showed a marked decline in the patients with abnormal values of CRP, ESR and fibrinogen, indirectly showing a decline in the inflammatory activity of psoriasis. more...
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- 2020
8. Diagnostics of HNSCC Patients: An Analysis of Cell Lines and Patient-Derived Xenograft Models for Personalized Therapeutical Medicine
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Ramona Gabriela Ursu, Ionut Luchian, Costin Damian, Elena Porumb-Andrese, Nicolae Ghetu, Roxana Gabriela Cobzaru, Catalina Lunca, Carmen Ripa, Diana Costin, Igor Jelihovschi, Florin Dumitru Petrariu, and Luminita Smaranda Iancu more...
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stomatognathic diseases ,stomatognathic system ,Clinical Biochemistry ,otorhinolaryngologic diseases ,neoplasms - Abstract
Head and neck squamous cell carcinomas (HNSCC) are very frequent worldwide, and smoking and chronic alcohol use are recognized as the main risk factors. For oropharyngeal cancers, HPV 16 infection is known to be a risk factor as well. By employing next-generation sequencing, both HPV-positive and negative HNSCC patients were detected as positive for PI3K mutation, which was considered an optimal molecular target. We analyzed scientific literature published in the last 5 years regarding the newly available diagnostic platform for targeted therapy of HNSCC HPV+/−, using HNSCC-derived cell lines cultures and HNSCC pdx (patient-derived xenografts). The research results are promising and require optimal implementation in the management of HNSCC patients. more...
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- 2022
9. Emerging Oncogenic Viruses in Head and Neck Cancers from Romanian Patients
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Ionut Luchian, Elena Porumb-Andrese, Victor Vlad Costan, Ovidiu Stamatin, Ramona Gabriela Ursu, Costin Damian, Octavian Dragos Palade, Luminita Smaranda Iancu, and Nicolae Ghetu
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Technology ,QH301-705.5 ,QC1-999 ,viruses ,criteria for carcinogenicity ,clinical utility ,medicine.disease_cause ,Herpesviridae ,oncogenic virus ,Multiplex polymerase chain reaction ,medicine ,General Materials Science ,Biology (General) ,Papillomaviridae ,Head and neck ,QD1-999 ,Instrumentation ,Genotyping ,Fluid Flow and Transfer Processes ,biology ,business.industry ,Physics ,Process Chemistry and Technology ,screening ,General Engineering ,Cancer ,Engineering (General). Civil engineering (General) ,biology.organism_classification ,medicine.disease ,Virology ,Computer Science Applications ,Polyomaviridae ,Chemistry ,TA1-2040 ,business ,Oncovirus - Abstract
(1) Background: Head and neck squamous cell carcinomas (HNSCCs) are some of the most frequent malignancies globally. Oncogenic viruses MCPyV, EBV and HPVs are recognized to be related to HNSCCs and skin cancers. There are no data from Romania regarding the involvement of herpes viruses and polyomaviruses in these types of cancer. We aim to evaluate the association of oncogenic viruses from Papillomaviridae, Herpesviridae, and Polyomaviridae families in HNSCCs and skin cancers. (2) Methods: A total of 26 fresh tumors (6/26 women) were tested for 67 viral agents using a multiplex PCR genotyping assay. (3) Results: A total of 23/26 (88.5%) samples were positive for one or more viruses. All the tested tumors were negative for any HPV (alpha or beta types). In total, we detected as positive samples: 16 (61.63%) EBV1, 12 (46.15%) HHV7, 8 (30.76%) MCV, 6 (23.07%) CMV and HHV6, 2 (7.69%) HHV8, 1 (3.8%) HPyV6 and EBV2. (4) Conclusions: We detected HPV-negative cases that are HPyV and HHV positive. In these fractions of HPV-negative HNSCCs cases, other oncogenic viruses may be involved, such as EBV1, MCV or CMV. Additional research is required for clarifying the natural history of these viruses in HNSCCs, as virus detection would have a decisive impact on diagnostic and decisional algorithms. more...
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- 2021
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10. The BRAF V600E Mutation Detection by quasa Sensitive Real-Time PCR Assay in Northeast Romania Melanoma Patients
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Elena Porumb-Andrese, Dan Ferariu, Luminita Smaranda Iancu, Ramona Gabriela Ursu, Delia Ciobanu, Costin Damian, Cristina Terinte, Irina Draga Căruntu, Iuliu Ivanov, and Vlad Porumb
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Oncology ,medicine.medical_specialty ,Technology ,QH301-705.5 ,QC1-999 ,Nodular melanoma ,Internal medicine ,medicine ,melanoma ,General Materials Science ,Target therapy ,Biology (General) ,Instrumentation ,neoplasms ,QD1-999 ,Fluid Flow and Transfer Processes ,business.industry ,BRAF V600E ,target therapy ,Process Chemistry and Technology ,Melanoma ,Physics ,General Engineering ,medicine.disease ,Engineering (General). Civil engineering (General) ,DNA extraction ,Computer Science Applications ,Chemistry ,Real-time polymerase chain reaction ,frequency ,Cutaneous melanoma ,Mutation (genetic algorithm) ,mutation ,TA1-2040 ,business - Abstract
Background: The prevalence of melanoma in Romanian patients is underestimated. There is a need to identify the BRAF V600E mutation to accurately treat patients with the newest approved BRAF inhibitor therapy. This is a pilot study in which we first aimed to choose the optimal DNA purification method from formalin fixation and paraffin embedding (FFPE) malignant melanoma skin samples to assess the BRAF mutation prevalence and correlate it with clinical pathological parameters. Methods: 30 FFPE samples were purified in parallel with two DNA extraction kits, a manual and a semi-automated kit. The extracted DNA in pure and optimum quantity was tested for the BRAF V600E mutation using the quantitative allele-specific amplification (quasa) method. quasa is a method for the sensitive detection of mutations that may be present in clinical samples at low levels. Results: The BRAF V600E mutation was detected in 60% (18/30) samples in patients with primary cutaneous melanoma of the skin. BRAFV600E mutation was equally distributed by gender and was associated with age >, 60, nodular melanoma, and trunk localization. Conclusions: The high prevalence of BRAF V600E mutations in our study group raises awareness for improvements to the national reporting system and initiation of the target therapy for patients with malignant melanoma of the skin. more...
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- 2021
11. The Optimal Management of Neisseria gonorrhoeae Infections
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Ramona Gabriela Ursu, Ionut Luchian, Costin Damian, Elena Porumb-Andrese, Roxana Gabriela Cobzaru, Giorgio Nichitean, Carmen Ripa, Diana Costin, Darius Sandu, Ioana-Maria Andrioaie, and Luminita Smaranda Iancu more...
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Microbiology (medical) ,Virology ,Microbiology - Abstract
Neisseria gonorrhoeae is one of the most frequent etiologic agents of STDs (sexually transmitted diseases). Untreated asymptomatic gonococcal infection in women can lead to spreading of the infection in the sexually active population and could lead to late consequences, such as sterility or ectopic pregnancies. One important issue about N. gonorrhoeae is its increasing resistance to antibiotics. This paper summarized the newest molecular antimicrobial resistance (AMR) detection assays for Neisseria gonorrhoeae connected with the latest therapeutic antimicrobials and gonococcal vaccine candidates. The assays used to detect AMR varied from the classical minimal inhibitory concentration (MIC) detection to whole-genome sequencing. New drugs against multi drug resistant (MDR) N. gonorrhoeae have been proposed and were evaluated in vivo and in vitro as being efficient in decreasing the N. gonorrhoeae burden. In addition, anti-N. gonorrhoeae vaccine candidates are being researched, which have been assessed by multiple techniques. With the efforts of many researchers who are studying the detection of antimicrobial resistance in this bacterium and identifying new drugs and new vaccine candidates against it, there is hope in reducing the gonorrhea burden worldwide. more...
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- 2022
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12. Sentinel Lymph Node Biopsy in Cutaneous Melanoma, a Clinical Point of View
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Daciana Elena Brănişteanu, Mihai Cozmin, Elena Porumb-Andrese, Daniel Brănişteanu, Mihaela Paula Toader, Diana Iosep, Diana Sinigur, Cătălina Ioana Brănişteanu, George Brănişteanu, Vlad Porumb, Alin Constantin Pînzariu, Sorin Liviu Băilă, and Alin Codruț Nicolescu more...
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Skin Neoplasms ,Sentinel Lymph Node Biopsy ,Humans ,Prospective Studies ,General Medicine ,Sentinel Lymph Node ,Melanoma ,Neoplasm Staging - Abstract
Sentinel lymph node biopsy (SLNB) is a surgical procedure that has been used in patients with cutaneous melanoma for nearly 30 years. It is used for both staging and regional disease control with minimum morbidity, as proven by numerous worldwide prospective studies. It has been incorporated in the recommendations of national and professional guidelines. In this article, we provide a summary of the general information on SLNB in the clinical guidelines for the management of cutaneous malignant melanoma (American Association of Dermatology, European Society of Medical Oncology, National Comprehensive Cancer Network, and Cancer Council Australia) and review the most relevant literature to provide an update on the existing recommendations for SLNB. more...
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- 2022
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13. Psoriasis—A Cancer Risk Factor?
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Laura Gheuca-Solovastru, Alina Ioana Halip, Andreea Cozma, Elena Porumb-Andrese, Dan Vâţă, and Adriana Ionela Patrascu
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Technology ,medicine.medical_specialty ,QH301-705.5 ,Colorectal cancer ,QC1-999 ,Prostate cancer ,Breast cancer ,Internal medicine ,Psoriasis ,medicine ,cancer ,General Materials Science ,Biology (General) ,Prospective cohort study ,QD1-999 ,Instrumentation ,risk ,Fluid Flow and Transfer Processes ,Bladder cancer ,business.industry ,Physics ,Process Chemistry and Technology ,General Engineering ,Cancer ,Odds ratio ,psoriasis ,Engineering (General). Civil engineering (General) ,medicine.disease ,Computer Science Applications ,Chemistry ,TA1-2040 ,business - Abstract
Psoriasis is not considered a strictly skin condition, but a complex disease with multisystem involvement due to the frequent associated comorbidities. We conducted a retrospective database study of 10,986 patients admitted in the interval January 2008–January 2019 to the Dermatology Clinic of the Iasi County “St. Spiridon” Emergency Hospital. Of the 10,986 patients admitted, 1288 were diagnosed with psoriasis. The association of malignancies was found in 40 of the psoriasis group cases and 399 of the control group cases that included various dermatological conditions. The calculation of Odds Ratios allowed us to determine if the patients with psoriasis could be at risk for certain malignancies. Thus, an association was suggested between psoriasis and central nervous system (CNS), upper aerodigestive tract cancer, endocrine cancer, bladder cancer, lung cancer, prostate cancer, breast cancer, or colorectal cancer. It is the first study of its kind in the northeastern region of Romania and can be the starting point for future long-term prospective cohort studies that will allow a more accurate data collection and a better understanding of the psoriasis–cancer relationship. more...
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- 2021
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14. Comprehensive review on the pathophysiology, clinical variants and management of pemphigus (Review)
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Cristina Popa, Elena Porumb-Andrese, Mădălina Florina Hâncu, Victor-Vlad Costan, and Mihaela Paula Toader
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Cancer Research ,medicine.medical_specialty ,pemphigus vulgaris ,Review ,paraneoplastic pemphigus ,Desmoglein ,Immunology and Microbiology (miscellaneous) ,immune system diseases ,medicine ,IgA pemphigus ,pemphigus foliaceus ,skin and connective tissue diseases ,Pemphigus herpetiformis ,Pemphigus foliaceus ,integumentary system ,business.industry ,Pemphigus vulgaris ,General Medicine ,pemphigus vegetans ,medicine.disease ,Dermatology ,Pemphigus ,Paraneoplastic pemphigus ,pemphigus herpetiformis ,Pemphigus vegetans ,business - Abstract
Pemphigus represents a group of chronic inflammatory disorders characterized by autoantibodies that target components of desmosomes, leading to the loss of intercellular adhesion between keratinocytes and causing intraepithelial blistering. The pemphigus group consists of four main clinical types with several variants: pemphigus vulgaris (with pemphigus vegetans and pemphigus herpetiformis as variants), pemphigus foliaceus, paraneoplastic pemphigus and IgA pemphigus (with two clinical variants: intraepidermal neutrophilic IgA dermatosis and subcorneal pustular dermatosis). Genetic factors are involved in the pathogenesis, with HLA-DR4 (DRB1*0402) and HLA-DRw6 (DQB1*0503) allele more common in patients with pemphigus vulgaris, HLA class II DRB1*0344 and HLA Cw*1445 correlated with paraneoplastic pemphigus, and HLA-DRB1*04:01, HLA-DRB1*04:06, HLA-DRB1*01:01, HLA-DRB1*14, associated with a higher risk of developing pemphigus foliaceus. Autoantibodies are conducted against structural desmosomal proteins in the skin and mucous membranes, mainly desmogleins, desmocollins and plakins. Cell-mediated immunity may also play a role, especially in paraneoplastic pemphigus. Patients may present erythema, blisters, erosions, and ulcers that may affect the skin, as well as mucosal surfaces of the oral cavity, eyes, nose, leading to severe complaints including pain, dysphagia, and fetor. Oral mucosal postbullous erosive lesions are frequently the first sign of disease in pemphigus vulgaris and in paraneoplastic pemphigus, without skin involvement, making the diagnosis difficult. Treatment options classically include immunosuppressive agents, such as corticosteroids and corticosteroid-sparing agents such as azathioprine, mycophenolate mofetil, cyclophosphamide, methotrexate or dapsone. Newer therapies focus on blocking cell signaling events induced by pathogenic autoantibodies and/or targeting specific autoantibodies. The disease evolution is conditioned by the treatment with maximum doses of corticosteroids and the side effects associated with long-term immunosuppressive therapy, which is why patients need a multidisciplinary approach in following the treatment. In this review, we provide a comprehensive overview of the epidemiology, pathophysiology, clinical aspect, diagnosis and management of the main intraepidermal blistering diseases from the pemphigus group. more...
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- 2021
15. 'Psycho-affective Implications in Dermatoaesthetics '
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Adriana Ionela Pătrașcu, Elena Porumb-Andrese, Ioana Adriana Popescu, Mihaela Paula Toader, Alina Ioana Halip, Laura Gheucă Solovăstru, Teona Asavei, and Dan Vâță
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Psychotherapist ,General Materials Science ,Psychology - Published
- 2019
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16. 'Pemphigus vulgaris triggered by psychological stress '
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Ioana Adriana Popescu, Elena Porumb-Andrese, Dan Vâță, Adriana Ionela Pătrașcu, Laura Gheucă Solovăstru, Alina Ioana Halip, Teona Asavei, and Mihaela Paula Toader
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medicine.medical_specialty ,business.industry ,Pemphigus vulgaris ,medicine ,Psychological stress ,General Materials Science ,business ,medicine.disease_cause ,medicine.disease ,Dermatology - Published
- 2019
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17. Host mRNA Analysis of Periodontal Disease Patients Positive for Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Tannerella forsythia
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Ramona Gabriela Ursu, Luminita Smaranda Iancu, Elena Porumb-Andrese, Costin Damian, Roxana Gabriela Cobzaru, Giorgio Nichitean, Carmen Ripa, Darius Sandu, and Ionut Luchian
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Inorganic Chemistry ,Organic Chemistry ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
Periodontal disease is a frequent pathology worldwide, with a constantly increasing prevalence. For the optimal management of periodontal disease, there is a need to take advantage of actual technology to understand the bacterial etiology correlated with the pathogenic mechanisms, risk factors and treatment protocols. We analyzed the scientific literature published in the last 5 years regarding the recent applications of mRNA analysis in periodontal disease for the main known bacterial species considered to be the etiological agents: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Tannerella forsythia. We identified new pathogenic mechanisms, therapeutic target genes and possible pathways to prevent periodontal disease. The mRNA analysis, as well as the important technological progress in recent years, supports its implementation in the routine management of periodontal disease patients. more...
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- 2022
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18. Kaposi Sarcoma, a Trifecta of Pathogenic Mechanisms
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Gabriela Rusu-Zota, Oana Mădălina Manole, Cristina Galeș, Elena Porumb-Andrese, Otilia Obadă, and Cezar Valentin Mocanu
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Clinical Biochemistry - Abstract
Kaposi’s sarcoma is a rare disease with four known variants: classic, epidemic, endemic and iatrogenic (transplant-related), all caused by an oncogenic virus named Human Herpes Virus 8. The viral infection in itself, along with the oncogenic properties of HHV8 and with immune system dysfunction, forms the grounds on which Kaposi’s Sarcoma may develop. Infection with HHV8 occurs through saliva via close contacts, blood, blood products, solid organ donation and, rarely, vertical transmission. Chronic inflammation and oncogenesis are promoted by a mix of viral genes that directly promote cell survival and transformation or interfere with the regular cell cycle and cell signaling (of particular note: LANA-1, v-IL6, vBCL-2, vIAP, vIRF3, vGPCR, gB, K1, K8.1, K15). The most common development sites for Kaposi’s sarcoma are the skin, mucocutaneous zones, lymph nodes and visceral organs, but it can also rarely appear in the musculoskeletal system, urinary system, endocrine organs, heart or eye. Histopathologically, spindle cell proliferation with slit-like vascular spaces, plasma cell and lymphocyte infiltrate are characteristic. The clinical presentation is heterogenic depending on the variant; some patients have indolent disease and others have aggressive disease. The treatment options include highly active antiretroviral therapy, surgery, radiation therapy, chemotherapy, and immunotherapy. A literature search was carried out using the MEDLINE/PubMed, SCOPUS and Google Scholar databases with a combination of keywords with the aim to provide critical, concise, and comprehensive insights into advances in the pathogenic mechanism of Kaposi’s sarcoma. more...
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- 2022
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19. Merkel Cell Polyoma Virus and Cutaneous Human Papillomavirus Types in Skin Cancers: Optimal Detection Assays, Pathogenic Mechanisms, and Therapeutic Vaccination
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Ramona Gabriela Ursu, Costin Damian, Elena Porumb-Andrese, Nicolae Ghetu, Roxana Gabriela Cobzaru, Catalina Lunca, Carmen Ripa, Diana Costin, Igor Jelihovschi, Florin Dumitru Petrariu, and Luminita Smaranda Iancu more...
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Microbiology (medical) ,Infectious Diseases ,General Immunology and Microbiology ,Immunology and Allergy ,Molecular Biology - Abstract
Oncogenic viruses are recognized to be involved in some cancers, based on very well-established criteria of carcinogenicity. For cervical cancer and liver cancer, the responsible viruses are well-known (e.g., HPV, HBV); in the case of skin cancer, there are still many studies which are trying to identify the possible viral etiologic agents as principal co-factors in the oncogenic process. We analysed scientific literature published in the last 5 years regarding mechanisms of carcinogenicity, methods of detection, available targeted therapy, and vaccination for Merkel cell polyomavirus, and beta human papillomavirus types, in relation to skin cancer. This review is targeted at presenting the recent findings which support the involvement of these viruses in the development of some types of skin cancers. In order to optimize the management of skin cancer, a health condition of very high importance, it would be ideal that the screening of skin cancer for these two analysed viruses (MCPyV and beta HPV types) to be implemented in each region’s/country’s cancer centres’ molecular detection diagnostic platforms, with multiplex viral capability, optimal sensitivity, and specificity; clinically validated, and if possible, at acceptable costs. For confirmatory diagnosis of skin cancer, another method should be used, with a different principle, such as immunohistochemistry, with specific antibodies for each virus. more...
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- 2022
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20. Use of imaging techniques for melanocytic naevi and basal cell carcinoma in integrative analysis (Review)
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Ioana Adriana Popescu, Laura Gheucă Solovăstru, Ioana-Alina Grajdeanu, Elena Porumb-Andrese, Alina Stîncanu, Dan Vata, Laura Stătescu, Maria Crisan, Tatiana Taranu, and Adriana Ionela Patrascu
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Reflectance confocal microscopy ,Cancer Research ,Melanocytic naevi ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,videodermoscopy ,Early detection ,Cancer ,General Medicine ,Review ,skin tumors ,medicine.disease ,imaging techniques ,Immunology and Microbiology (miscellaneous) ,Biopsy ,medicine ,Basal cell carcinoma ,high frequency ultrasonography ,Radiology ,Skin cancer ,Ultrasonography ,business ,integrative analysis - Abstract
Early detection of skin cancer is essential in order to obtain an improved prognosis. Clinicians need more objective and non-invasive examination methods to support their decision whether to biopsy or not tumoral lesions. These may include several imaging techniques such as dermoscopy, videodermoscopy, also known as sequential digital dermoscopy (SDD), computer-aided diagnosis (CAD), total body photography, imaging and high-frequency ultrasonography (HFUS), reflectance confocal microscopy, multiphoton tomography, electrical impedance spectroscopy, Raman spectroscopy, stepwise two-photon-laser spectroscopy and quantitative dynamic infrared. This review summarizes the current developments in the field of melanocytic lesions, such as naevi and basal cell carcinoma (BCC) imaging techniques. The aim was to collect and analyze data concerning types, indications, advantages and disadvantages of modern imaging techniques for in vivo skin tumor diagnosis. Two main methods were focused on, namely videodermoscopy and HFUS, which can be included in daily dermatologists' practice. In skin tumors HFUS allows the assessment of tumoral lesions with depth smaller than 1.5 cm, being described a correlation between ultrasonographic depth and the histologic index. more...
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- 2020
21. Understanding the Pattern of Oropharyngeal Cancers from North-East Romanian Patients
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Ramona Ursu, Simona Giusca, Irene Spiridon, Bianca Manole, Mihai Danciu, Victor Costan, Dragos Palade, Nicolae Ghetu, Paula Toader, Mădălina Vlad, Costin Damian, Elena Porumb-Andrese, Ionut Luchian, and Luminița Iancu more...
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Fluid Flow and Transfer Processes ,HPV ,Technology ,QH301-705.5 ,viruses ,Physics ,QC1-999 ,Process Chemistry and Technology ,General Engineering ,biomarkers ,Engineering (General). Civil engineering (General) ,survival ,Computer Science Applications ,Chemistry ,cancer ,early detection ,General Materials Science ,TA1-2040 ,Biology (General) ,QD1-999 ,Instrumentation - Abstract
Background: Human papilloma virus (HPV) is acknowledged as a risk factor for oropharyngeal squamous cellular cancers (OPSCC), of which the dominant types are tonsillar (TSCC) and base of tongue cancer (BOTSCC). Objective: To assess the role of HPV in selected OPSCC cases, from Romanian patients by sensitive and complementary molecular assays. Material and Methods: Fifty-four formalin fixed paraffin embedded (FFPE) OPSCC samples were analyzed for HPV DNA by a PCR-based bead-based multiplex-assay. Thirty-four samples were tested for HPV RNA and for overexpression of p16INK4a by immunohistochemistry. Twenty samples were evaluated by Competitive Allele-Specific Taqman PCR (CAST-PCR) for fibroblast growth factor receptor 3 protein (FGFR3) status. Results: A total of 33.3% (18/54) OPSCC samples were positive for HPV DNA. HPV16 was the most frequent type (30%, 16/54); followed by HPV18 (3.7%, 2/54); and 1 sample (1.8%) was positive for both HPV16 and 18. HPV18 E6*I was detected in a HPV18 DNA-positive oropharynx tumor. Four samples positive for HPV16 were also positive for p16INK4a. All the tested samples were negative for FGFR3. Conclusions: The increased HPV16 prevalence is in line with similar studies and is a new confirmation that HPV16 is the most prevalent type in our country; supporting the potential benefit of prophylactic vaccines. Overall, there is no concordance between DNA and any of the two other analytes that are considered being markers of HPV-driven cancers. There is a need to explore novel screening strategies that could be broadly used in the clinical routine to initiate preventive measures. more...
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- 2021
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22. Molecular Profile of Skin Cancer
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Elena Porumb-Andrese, Ramona Gabriela Ursu, Ioana Mârţu, Mihaela Monica Scutariu, Thomas Gabriel Schreiner, Cosmin Gabriel Popa, Darius Sandu, Vlad Porumb, and Ionut Luchian
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squamous cell carcinoma ,Technology ,QH301-705.5 ,QC1-999 ,Gene mutation ,Molecular level ,Immune system ,basal cell carcinoma ,melanoma ,medicine ,General Materials Science ,Basal cell carcinoma ,Biology (General) ,QD1-999 ,Instrumentation ,gene mutations ,Fluid Flow and Transfer Processes ,integumentary system ,business.industry ,Physics ,Process Chemistry and Technology ,Melanoma ,General Engineering ,Engineering (General). Civil engineering (General) ,medicine.disease ,Computer Science Applications ,Chemistry ,Cancer research ,Molecular Profile ,TA1-2040 ,Skin cancer ,business - Abstract
Neoplasia occurs as a result of genetic mutations. Research evaluating the association between gene mutations and skin cancer is limited and has produced inconsistent results. There are no established guidelines for screening skin cancer at molecular level. It should also be noted that the combinations of some mutations may play a role in skin tumors’ biology and immune response. There are three major types of skin cancer, and the originality of this study comes from its approach of each of them. more...
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- 2021
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23. Pemphigus vulgaris - approach and management (Review)
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Laura Stătescu, Ioana-Alina Grajdeanu, Elena Porumb-Andrese, Dan Vata, Laura Gheucă Solovăstru, Adriana Ionela Patrascu, and Ioana Adriana Popescu
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0301 basic medicine ,Cancer Research ,integumentary system ,medicine.diagnostic_test ,business.industry ,Pemphigus vulgaris ,Autoantibody ,Azathioprine ,General Medicine ,medicine.disease ,Desmoglein ,03 medical and health sciences ,Pemphigus ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Immunology and Microbiology (miscellaneous) ,030220 oncology & carcinogenesis ,Immunology ,Biopsy ,medicine ,Rituximab ,Oral mucosa ,business ,medicine.drug - Abstract
The place of pemphigus vulgaris (PV) among autoimmune bullous dermatoses is well known. In pemphigus, IgG autoantibodies are directed against desmogleins 1 and 3, which are part of the cadherin family of cell-cell adhesion molecules. These structures are responsible for maintaining the intercellular adherence in stratified squamous epithelia, such as the skin and oral mucosa. The incidence of autoimmune bullous dermatoses is steadily increasing, being associated with a high degree of morbidity. The pathophysiology of these dermatoses is very well understood, complemented by recent genetic studies. The gold standard for the diagnosis of pemphigus vulgaris is the detection of autoantibodies or complement component 3 by direct immunofluorescence microscopy of a perilesional biopsy. Early diagnosis and initiation of treatment are necessary in order to achieve a favorable prognosis. Although the first line of treatment is corticotherapy, there are no clear guidelines on dosing regimens, and long-term adverse effects are important. Corticosteroid-sparing adjuvant therapies have been employed in the treatment of PV, aiming to reduce the necessary cumulative dose of corticosteroids. In addition, therapies with anti-CD20 antibodies are used, but antigen-specific immune suppression-based treatments represent the future. more...
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- 2019
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24. Association between personality type, affective distress profile and quality of life in patients with psoriasis vs. patients with cardiovascular disease
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Laura Stătescu, Ioana Adriana Popescu, Elena Porumb-Andrese, Dan Vâță, Roxana Postolică, Cristian Stătescu, Alina-Ioana Grăjdeanu, Adriana Ionela Pătrașcu, and Laura Gheucă Solovăstru
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0301 basic medicine ,Cancer Research ,media_common.quotation_subject ,Dysfunctional family ,Disease ,Emotional Instability ,03 medical and health sciences ,0302 clinical medicine ,Quality of life (healthcare) ,Immunology and Microbiology (miscellaneous) ,Psoriasis ,Medicine ,Personality ,media_common ,business.industry ,Articles ,psoriasis ,General Medicine ,affective distress profile ,medicine.disease ,cardiovascular diseases ,030104 developmental biology ,quality of life ,Personality type ,personality ,030220 oncology & carcinogenesis ,Anxiety ,medicine.symptom ,business ,Clinical psychology - Abstract
Common chronic conditions, such as cardiovascular diseases and psoriasis, are associated with increased psychological distress. Health-related quality of life and personality type in patients with these two diseases were evaluated, including the patient's ability to respond truthfully or his/her tendency to be introverted or extroverted. The subjects (n=63) responded to questionnaires including: SF-36 questionnaire, Eysenck test and the questionnaire of the affective distress profile designed to assess the subjective dimension of functional negative emotions, dysfunctional negative emotions and positive emotions. Psoriasis patients had significantly higher average scores of physical functions and limitations brought on by emotional problems. Emotional instability was common for both types of patients and anxiety was associated with a low level of education. Overall, we believe that it is necessary to include items evaluating the patients' affective profile and personality in the assessment protocols of these diseases. These items could help the clinician in identifying possible factors that signal worsening of the disease or of non-response to treatment. more...
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- 2019
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25. Design and Testing of an Experimental Steam-Induced Burn Model in Rats
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Vlad Porumb, Elena Porumb-Andrese, Alexandru Florentin Trandabăț, Mihail Gabriel Dimofte, Cristina Terinte, Irina Draga Căruntu, and Dragos Pieptu
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medicine.medical_specialty ,Hot Temperature ,Article Subject ,lcsh:Medicine ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Humans ,Rats, Wistar ,Process engineering ,Wound Healing ,General Immunology and Microbiology ,business.industry ,lcsh:R ,030208 emergency & critical care medicine ,General Medicine ,Surgery ,Rats ,Disease Models, Animal ,Steam ,030220 oncology & carcinogenesis ,Environmental science ,business ,Burns ,Research Article - Abstract
Background. Most of the current models for experimental burns pose difficulties in ensuring consistency and standardization.Aim of Study. We aimed to develop an automated, reproducible technique for experimental burns using steam-based heat transfer.Methods. The system developed for steam exposure was based on a novel, integrated, computer-controlled design. Three groups of rats were exposed to steam for 1, 3, and 7 seconds. The lesions were evaluated after 20 minutes, 48 hours, and 72 hours after burn induction.Results. One-second steam application produced a superficial second-degree burn; three-second application induced deep second-degree burn; and seven-second application led to a third-degree burn.Conclusion. The high level of automation of our integrated, computer-controlled system makes the difference between our system and other models, by ensuring the control of the duration of exposure, temperature, and pressure and eliminating as many potential human generated errors as possible. The automated system can accurately reproduce specific types of burns, according to histological assessment. This model could generate the reproducible data needed in the study of burn pathology and in order to assess new treatments. more...
- Published
- 2017
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