Search

Quantitation and morphometric parameters of bone marrow MK in rats during acute haemorrhagic shock were analysed. The results were compared with group of normal rats and rats after surgical manipulation. In examining animals statistical significant differences were observed in localization, size, form factor, N/C ratio, forming cluster forms and phenomena of emperipolesis. These disturbances of MK were often observed in rats after surgical manipulation. The results indicate that the changes of MK have just observed after 60 min. from indication of haemorrhagic shock.

The authors presented the morphometrical evaluation of the bone marrow MK in rats in the acute stage of experimental hemorrhagic shock. The experiments used a semiautomatic computer programme. The total number of MK per 1 mm2 of the bone marrow was analysed with regard to various morphological forms of MK, their surface area, shape disorders, nuclear-cytoplasmic ratio and cluster forms. A considerable shift was observed in the MK parameters examined, suggesting a significant effect of hemorrhagic shock upon megakaryocytopoiesis.

The activity of rat peritoneal cells were assessed by the phorbol mirystinian acetate (PMA)-induced luminol chemiluminescence (LCL). Results in control groups (0 - no manipulation, and I - carotid artery cannulation) were compared with those in the untreated hemorrhagic shock (group II), in the shock treated with the standard polyelectrolyte solution (PES) (group III), and in shock treated with PAF receptor-antagonist BN 52021 + PES (group IV). The maximal and the most rapid LCL was observed in the group treated with BN 52021 (group IV), while chemiluminescent response in the the untreated shock (group II) and in shock treated with PES was minimally expressed and late. The findings indicate for a rapid activation of peritoneal cells during ca 1 hour of hemorrhagic shock. This leads to exhausting their ability to the superoxide anion generation 15 minutes later. Peritoneal cells obtained from the group treated with the BN 52021 revealed a preserved ability to the respiratory burst. It can be concluded that BN 52021 effectively inhibits activation of the PC during hemorrhagic shock.