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Graphical abstract Trace (A) shows a typical transient [Ca2+]i response induced by 50 mM K+ observed in an AVP-eGFP neurone (inset), Trace (B) shows the spontaneous [Ca2+]i oscillations observed in an AVP-eGFP neurone (inset). The corresponding changes in the fluorescence are shown in C and D, respectively., Highlights • Supraoptic fluorescent vasopressin (AVP-eGFP) and oxytocin (OT-mRFP1) neurones exhibit distinct spontaneous [Ca2+]i oscillations. • Vasopressin triggers [Ca2+]i oscillations, intensifies existing oscillations, and exceptionally stops oscillations. • Hyper- or hypo-osmotic stimuli have an intensifying or inhibitory effect on oscillations, respectively. • Nearly 90% of neurones from 3 or 5-day-dehydrated rats exhibit oscillations. • More than 80% of OT-mRFP1 neurones from 3 to 6-day-lactating rats are oscillatory vs. about 44% in virgins., The magnocellular vasopressin (AVP) and oxytocin (OT) neurones exhibit specific electrophysiological behaviour, synthesise AVP and OT peptides and secrete them into the neurohypophysial system in response to various physiological stimulations. The activity of these neurones is regulated by the very same peptides released either somato-dendritically or when applied to supraoptic nucleus (SON) preparations in vitro. The AVP and OT, secreted somato-dendritically (i.e. in the SON proper) act through specific autoreceptors, induce distinct Ca2+ signals and regulate cellular events. Here, we demonstrate that about 70% of freshly isolated individual SON neurones from the adult non-transgenic or transgenic rats bearing AVP (AVP-eGFP) or OT (OT-mRFP1) markers, produce distinct spontaneous [Ca2+]i oscillations. In the neurones identified (through specific fluorescence), about 80% of AVP neurones and about 60% of OT neurones exhibited these oscillations. Exposure to AVP triggered [Ca2+]i oscillations in silent AVP neurones, or modified the oscillatory pattern in spontaneously active cells. Hyper- and hypo-osmotic stimuli (325 or 275 mOsmol/l) respectively intensified or inhibited spontaneous [Ca2+]i dynamics. In rats dehydrated for 3 or 5 days almost 90% of neurones displayed spontaneous [Ca2+]i oscillations. More than 80% of OT-mRFP1 neurones from 3 to 6-day-lactating rats were oscillatory vs. about 44% (OT-mRFP1 neurones) in virgins. Together, these results unveil for the first time that both AVP and OT neurones maintain, via Ca2+ signals, their remarkable intrinsic in vivo physiological properties in an isolated condition.

The main long-range goal of this study is to analyse how electrical activity generated at somata is transformed into chemical signals at nerve terminals. We try to achieve this goal by examining, at the level of membrane and molecular mechanisms, the steps considered to be involved in stimulus-secretion coupling: how neurotransmitters are released in response to depolarisation of the nerve terminal membrane. We have demonstrated over several years the release of the neuroactive peptides, vasopressin and oxytocin and the role for Ca(2+), in the hypothalamic-neurohypophysial system (HNS) of the rat and also in cardiac tissues (from the brain to the heart). This study was performed using both a well-characterized preparation of pure, isolated neurohypophysial nerve terminals, a preparation of isolated hypothalamic magnocellular neurones and isolated cardiac myocytes. Furthermore, the intact HNS would affords the unique opportunity of comparing the somata and terminals of the same CNS neurones. This article plans to build on the this wealth of information already gathered on isolated, individual terminals/somata in order to analysis of the physiology of the whole, intact system in situ. We show some of the well established data to explain: i) why are different patterns of electrical activity (i.e. bursts) best for AVP vs. OT release in the intact HNS, ii) are there any other parameters, transmitters, messengers, hormones and drugs that could play an important role, iii) is Ca(2+) important to understand this physiology, and finally iv) what do we learn from the comparison to the cardiac system?