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Two central bearded dragons ( Pogona vitticeps), a 3-y-old male and a 5-y-old female, were diagnosed with different manifestations of lymphoma at the Kansas State Veterinary Diagnostic Laboratory between 2019 and 2020. The 3-y-old male was presented for postmortem evaluation and was in poor body condition. Microscopically, nearly all examined organs contained variable numbers of neoplastic round cells. Neoplastic cells in the stomach and liver had moderate immunoreactivity to CD3 consistent with multicentric T-cell lymphoma, and non-neoplastic lymphocytes infiltrating the stomach mass had strong immunoreactivity to Pax5. The 5-y-old female had an ulcerated oral mass located in the right lingual gingiva submitted as an excisional biopsy. Microscopically, the mass was composed of large numbers of neoplastic round cells in the epithelium and connective tissue that were strongly and diffusely positive for CD3 and frequently positive for Pax5, consistent with a dual-positive, localized, epitheliotropic T-cell lymphoma. Neoplastic and non-neoplastic lymphocytes did not stain with CD20 or CD79a. Neoplasms are increasingly reported as a cause of morbidity and mortality in reptiles. Our 2 cases illustrate various presentations of T-cell lymphoma and the effectiveness of CD3 and Pax5 immunohistochemistry in bearded dragons.

Objectives The GnRH analogue, deslorelin, is commonly used for hormonal suppression in a wide range of species. Case Description A 5-year-old mixed breed spayed female pet rabbit (Oryctolagus cuniculus) presented for evaluation of behavioral changes including inappropriate urination and sexual aggression. Abdominal ultrasonography revealed left adrenal enlargement with a nodule located on the cranial pole. Increased concentrations of serum testosterone suggested adrenal pathology and hyperandrogenism. Subcutaneous placement of a 4.7 mg deslorelin implant intrascapularly resulted in lower testosterone levels, suppressed clinical signs, and decreased adrenal gland size. As of the submission of this paper (13 months later), presenting clinical signs have not returned and testosterone levels continue to remain in the normal reference range. Conclusion This implant was successful at suppressing presenting clinical signs and lowering serm testosterone levels. Case Relevance The clinical experience from this case suggests that this deslorelin implant may be used to manage clinical signs in similar cases where adrenalectomy may not be an option. The use of this implant in pet rabbits should be considered where it is ethically and legally allowed.

OBJECTIVE To evaluate the effects of a dexmedetomidine-midazolam-ketamine (DMK) combination administered IM to captive red-footed tortoises (Chelonoidis carbonaria). ANIMALS 12 healthy adult red-footed tortoises. PROCEDURES In a prospective experimental study, DMK (0.1, 1.0, and 10 mg/kg, respectively) was administered IM as separate injections into the right antebrachium. Atipamezole (0.5 mg/kg, IM) and flumazenil (0.05 mg/kg, SC) were administered into the left antebrachium 60 minutes later. Times to the first treatment response and maximal treatment effect after DMK administration and time to recovery after reversal agent administration were recorded. Vital signs and reflexes or responses to stimuli were assessed and recorded at predetermined intervals. RESULTS DMK treatment produced deep sedation or light anesthesia for ≥ 20 minutes in all tortoises. Induction and recovery were rapid, with no complications noted. Median times to first response, maximum effect, and recovery were 4.5, 35, and 14.5 minutes, respectively. Two tortoises required additional reversal agent administration but recovered < 20 minutes after the repeated injections. Mean heart and respiratory rates decreased significantly over time. All animals lost muscle tone in the neck and limbs from 35 to 55 minutes after DMK injection, but other variables including palpebral reflexes, responses to mild noxious stimuli (eg, toe pinching, tail pinching, and saline ([0.9 NaCl] solution injection), and ability to intubate were inconsistent. CONCLUSIONS AND CLINICAL RELEVANCE DMK administration produced deep sedation or light anesthesia with no adverse effects in healthy adult red-footed tortoises. At the doses administered, deep surgical anesthesia was not consistently achieved. Anesthetic depth must be carefully evaluated before performing painful procedures in red-footed tortoises with this DMK protocol.

Urolithiasis represents a common, potentially life-threatening condition in pet guinea pigs (Cavia porcellus). The purpose of this study is to determine the potential association between signalment, packed cell volume, total solids, selected biochemical parameters, and imaging evidence of urolithiasis in client-owned guinea pigs presented to two veterinary teaching hospitals. Medical record databases from two veterinary teaching hospitals were searched for cases of client-owned guinea pigs that had both a plasma biochemistry panel and an imaging study performed during one medical event. Other parameters including age, sex, weight, packed cell volume, and total solids were also recorded. Of the 81 guinea pigs that met the inclusion criteria, 32 animals (40%) had evidence of urolithiasis present on at least one of their imaging modalities. The odds for the imaging presence of urolithiasis increased with a decreasing packed cell volume, an increasing plasma creatinine concentration, and with a decreasing plasma phosphorus concentration. While these variables were statistically significant and associated with imaging presence of urolithiasis, they had low predictive value to discriminate between guinea pigs with and without imaging evidence of urolithiasis. Given the relatively high prevalence of urolithiasis in guinea pigs in this study, imaging is strongly recommended for every animal with suspected affliction.

OBJECTIVE To determine the agreement between plasma total solids (TS) concentration as measured by refractometry and plasma total protein (TP) concentration as measured by biuret assay in pet rabbits and ferrets. SAMPLE 253 and 146 blood samples from 146 and 121 ferrets and rabbits, respectively, with results of CBC and plasma biochemical analyses. PROCEDURES Data were collected from medical records regarding plasma TS and TP concentrations, PCV, plasma biochemical values, plasma appearance, and patient signalment. Agreement was determined between refractometer and biuret assay (reference method) values for plasma TS and TP concentration. Other variables were examined for an impact on this agreement. RESULTS Mean ± SD plasma TP and TS concentrations were 6.4 ± 0.8 mg/dL and 6.6 ± 0.8 mg/dL, respectively, for rabbits and 6.3 ± 1.2 mg/dL and 6.4 ± 1.1 mg/dL for ferrets. On average, refractometer values overestimated plasma TP concentrations as measured by biuret assay. Plasma cholesterol, glucose, and BUN concentrations and hemolysis and lipemia had significant effects on this bias for ferrets; only BUN concentration had an effect on bias for rabbits given the available data. Other variables had no influence on bias. The limits of agreement were wider than the total allowable analytic error, and > 5% of the data points were outside acceptance limits, indicating that the 2 methods were not in clinical agreement. CONCLUSIONS AND CLINICAL RELEVANCE Refractometer measurements of plasma TS concentration failed to provide a good estimation of biuret assay measurements of plasma TP concentration in rabbits and ferrets, suggesting that these 2 analytic methods and the results they yield cannot be used interchangeably in these species.

A 4-year-old female spayed domestic ferret (Mustela putorius furo) presented with a history of vomiting over 24 hours. On physical examination, a significantly enlarged, firm spleen was palpated. Abdominal radiographs and abdominal ultrasound were suggestive of a splenic torsion or splenic infarction. An exploratory laparotomy confirmed the initial diagnosis and splenectomy was performed using a vessel sealing device. Histologic evaluation and culture of the spleen were consistent with primary torsion without evidence of infection or neoplasia. The patient recovered from surgery without complications. Based on a literature search, this is the first report of the clinical diagnosis and successful surgical treatment of a primary splenic torsion in a ferret. Although it appears to be a rare and potentially life-threatening disease in ferrets, splenic torsion should be considered as a differential diagnosis in ferrets that present with non-specific signs and a palpably enlarged spleen.

OBJECTIVE To evaluate and compare the anesthetic effects of alfaxalone-ketamine-midazolam (AKM) and alfaxalone-ketamine-dexmedetomidine (AKD) in black-tailed prairie dogs (Cynomys ludovicianus). ANIMALS 9 male black-tailed prairie dogs. PROCEDURES Prairie dogs were anesthetized with AKM (6 mg/kg alfaxalone, 30 mg/kg ketamine, and 1.5 mg/kg midazolam) and AKD (6 mg/kg alfaxalone, 30 mg/kg ketamine, and 0.15 mg/kg dexmedetomidine) in a prospective, complete cross-over study. Atipamezole (1.5 mg/kg) after AKD or flumazenil (0.1mg/kg) after AKM was administered 45 minutes after induction of anesthesia. Onset of general anesthesia, physiologic parameters, depth of anesthesia, and time to recovery after reversal administration were evaluated for each treatment. RESULTS Both AKM and AKD produced a deep plane of anesthesia in black-tailed prairie dogs that varied in duration. The median induction times for AKM and AKD were 82 and 60 seconds, respectively. The median recovery times for AKM and AKD were 27 and 21 minutes, respectively. There were no significant differences between protocols for induction (P = .37) and recovery (P = .51) times. All measured reflexes were absent in all animals at 5 minutes postinduction, with hindlimb reflexes returning prior to forelimb reflexes. Heart rate was lower but respiratory rate was higher in the AKD treatment. Body temperature decreased significantly for both protocols (P < .001) and was significantly lower with AKM than AKD (P < .001). CLINICAL RELEVANCE Both AKM and AKD produced a deep plane of anesthesia in black-tailed prairie dogs. For both protocols, heat support and oxygen support are indicated.

American flamingos (Phoenicopterus ruber) are commonly kept in zoological collections and are important in research, conservation awareness, and education. Hematology and biochemistry parameters are reported to change with age in many long-lived species. A retrospective analysis of recorded data (147 samples of PCV/TS and plasma biochemistry) from n = 16 (F = 7, M = 9) zoo-kept flamingos (1–16 years) was performed. Blood samples were collected as part of routine annual health evaluation over the course of 11 years. The effect of aging and sex on biochemical analytes in individuals over time was assessed. Most analytes did not differ between sexes. Males had higher TS, and lower sodium, chloride, and ALT concentrations when controlling for age. The only variable with a significant sex × age interaction was ALP, which only changed with age in females. Most analytes did not differ with age. PCV, UN, and Na:K ratio increased, while potassium, ALP, and uric acid concentrations decreased with each year of life. While aging influenced select biomarkers in the current study, the clinical significance has yet to be determined, and further studies including a larger age range are recommended.

A 10-week-old, intact male miniature potbellied pig (Sus scrofa) presented for intense pruritus that had started approximately 2 weeks earlier. Following microscopic identification of multiple stages of the Sarcoptes scabiei, var. suis from its skin,the pig was treated with ivermectin (300-400 μg/kg subcutaneously), three times at 10-day intervals.

A 27-year-old female African grey parrot (

Providing safe anesthetic events in ferrets can be achieved if fundamental principles in anesthesia are followed. Each phase of the anesthetic, event including preanesthetic, maintenance, and postanesthetic phase, have certain considerations. The anesthetic supervisor or veterinarian providing management should have a firm understanding of the species-specific anatomy, physiology, and common indications of general anesthesia along with perspective of their own experience with ferrets. Ensuring these guidelines are followed will facilitate safe administration of general anesthesia in this species.

To evaluate the effects of a dexmedetomidine-midazolam-ketamine (DMK) combination administered IM to captive red-footed tortoises (12 healthy adult red-footed tortoises.In a prospective experimental study, DMK (0.1, 1.0, and 10 mg/kg, respectively) was administered IM as separate injections into the right antebrachium. Atipamezole (0.5 mg/kg, IM) and flumazenil (0.05 mg/kg, SC) were administered into the left antebrachium 60 minutes later. Times to the first treatment response and maximal treatment effect after DMK administration and time to recovery after reversal agent administration were recorded. Vital signs and reflexes or responses to stimuli were assessed and recorded at predetermined intervals.DMK treatment produced deep sedation or light anesthesia for ≥ 20 minutes in all tortoises. Induction and recovery were rapid, with no complications noted. Median times to first response, maximum effect, and recovery were 4.5, 35, and 14.5 minutes, respectively. Two tortoises required additional reversal agent administration but recovered20 minutes after the repeated injections. Mean heart and respiratory rates decreased significantly over time. All animals lost muscle tone in the neck and limbs from 35 to 55 minutes after DMK injection, but other variables including palpebral reflexes, responses to mild noxious stimuli (eg, toe pinching, tail pinching, and saline ([0.9 NaCl] solution injection), and ability to intubate were inconsistent.DMK administration produced deep sedation or light anesthesia with no adverse effects in healthy adult red-footed tortoises. At the doses administered, deep surgical anesthesia was not consistently achieved. Anesthetic depth must be carefully evaluated before performing painful procedures in red-footed tortoises with this DMK protocol.

A 1-year-old, female intact crested gecko was evaluated because of skin discoloration on her ventrum. Full-thickness biopsies were taken and evaluated histologically. Multifocally and regularly, there were collections of birefringent, granular material, localized primarily to the most superficial dermis subjacent to the overlying epithelium, throughout the length of examined sections. Polarized illumination revealed birefringent iridophores. This case either represents a benign aggregation of iridophores or simply regions of variable depigmentation, which may be considered as differential diagnoses when geckos are presented with similar clinical signs.

Background: Ventral abdominal herniation (with or without oviduct involvement) in avian species is most commonly seen in reproductively active females. Case Description: A 2.5-year-old female budgerigar (Melopsittacus undulatus) presented for a ventral tail, 2.0 cm in diameter, mass extending between the cloaca and the tail base. Upon surgical evaluation, the mass was determined to be a herniation of the oviduct and later confirmed with histopathology. A salpingohysterectomy and closure of the body wall defect was performed, and the patient recovered well. Postoperative radiography revealed no evidence of retained egg or other obvious pathology to determine an underlying cause. Conclusions and Case Relevance: Lateral body wall herniations with involvement of the oviduct has been reported, but an undertail body wall herniation involving the oviduct has not been previously reported based on the literature search, and should be considered in birds presented with similar lesions in this anatomical location.

Two newly acquired, one-month-old, intact male pet domestic rats (Rattus norvegicus) that were housed together presented for a wellness examination. One of the rats had malodorous, green-colored, soft feces. A pooled microscopic fecal examination demonstrated presence of Eimeria oocysts. Both rats were treated individually with ponazuril (30 mg/kg p.o.) administered in two treatments 48 hours apart. Three weekly pooled fecal samples following treatment were negative, suggesting clearing of the infection. No adverse clinical signs were noted. Most reported anticoccidian treatments in rodents describe prolonged treatment protocols or administration of treatment in the water, which can result in unnecessary stress and handling, or ineffective and uncontrolled level of drug administration, respectively. This is the first report of the favorable clinical experience of this treatment protocol, suggesting it is efficacious, easy to administer, and safe when treating similar infections in rats.

OBJECTIVE To evaluate the utility of commercially available reagent test strips for estimation of BUN concentration and detection of azotemia in pet rabbits (Oryctolagus cuniculus) and ferrets (Mustela putorius furo). SAMPLE 65 blood samples from 53 rabbits and 71 blood samples from 50 ferrets of various health statuses. PROCEDURES BUN concentrations were measured with a clinical laboratory biochemical analyzer and estimated with a reagent test strip. Results obtained with both methods were assigned to a BUN category (range, 1 to 4; higher categories corresponded to higher BUN concentrations). Samples with a biochemical analyzer BUN concentration ≥ 27 mg/dL (rabbits) or ≥ 41 mg/dL (ferrets) were considered azotemic. A test strip BUN category of 3 or 4 (rabbits) or 4 (ferrets) was considered positive for azotemia. RESULTS Test strip and biochemical analyzer BUN categories were concordant for 46 of 65 (71%) rabbit blood samples and 58 of 71 (82%) ferret blood samples. Sensitivity, specificity, and accuracy of the test strips for detection of azotemia were 92%, 79%, and 82%, respectively, for rabbit blood samples and 80%, 100%, and 96%, respectively, for ferret blood samples. CONCLUSIONS AND CLINICAL RELEVANCE Test strips provided reasonable estimates of BUN concentration but, for rabbits, were more appropriate for ruling out than for ruling in azotemia because of false-positive test strip results. False-negative test strip results for azotemia were more of a concern for ferrets than rabbits. Testing with a biochemical analyzer remains the gold standard for measurement of BUN concentration and detection of azotemia in rabbits and ferrets.

A 3-year-old, male intact, pet inland bearded dragon (Pogona vitticeps) presented with a history of diarrhea, progressive inappetence and weight loss. A palpable cranial celomic mass was identified on physical examination and confirmed to be hepatic in origin by celomic ultrasonography. Hematologic and biochemical abnormalities were mild and consistent with inflammation, regenerative anemia, and hepatocellular injury. Fine needle aspiration of the liver masses was suggestive of amoebiasis and the patient was humanely euthanized. PCR and Sanger DNA sequencing of liver aspirates were supportive of Entamoeba infection, although definitive speciation was not possible. Pathogenic amoebiasis due to infection by E. invadens has been reported in a wide range of reptiles and is an important cause of morbidity and mortality in these species.

OBJECTIVE To evaluate efficacy and safety of anesthesia with dexmedetomidine-ketamine-midazolam (DKM) in five-striped palm squirrels (Funambulus pennantii). ANIMALS 8 male squirrels. PROCEDURES Squirrels were anesthetized with DKM (dexmedetomidine, 0.1 mg/kg; ketamine hydrochloride, 30 mg/kg; and midazolam, 0.75 mg/kg) administered IM. Atipamezole (0.15 mg/kg) and flumazenil (0.1 mg/kg) were administered IM 40 minutes after induction of anesthesia. Vital signs and responses were recorded every 5 minutes during anesthesia. RESULTS Anesthetic induction and recovery from anesthesia were rapid and without complications in all squirrels. Median anesthetic induction time was 67.5 seconds (interquartile [25th to 75th percentile] range, 5.5 seconds), and mean ± SD recovery time after drug reversal was 147 ± 79 seconds. Heart rate, respiratory rate, and rectal temperature significantly decreased during the anesthetic period. All squirrels became hypothermic by 40 minutes after induction. The righting reflex was absent during the 40-minute anesthetic period in all squirrels, with variable responses for the palpebral reflex, jaw tone, forelimb withdrawal reflex, and hind limb withdrawal reflex. Only 2 of 8 squirrels had loss of the limb withdrawal reflex in both the forelimbs and hind limbs from anesthetic induction to 25 minutes after induction. CONCLUSIONS AND CLINICAL RELEVANCE DKM appeared to provide safe and effective anesthesia in five-striped palm squirrels, but oxygen and thermal support were indicated. At the doses administered, deep surgical anesthesia was not consistently achieved, and anesthetic depth of individual squirrels must be determined before surgical procedures are performed in palm squirrels anesthetized with this drug combination.

OBJECTIVE To compare anesthetic effects of alfaxalone-ketamine-dexmedetomidine (AKD) and alfaxalone-butorphanol-midazolam (ABM) in naked mole-rats (Heterocephalus glaber). ANIMALS 20 naked mole-rats. PROCEDURES Naked mole-rats received AKD (alfaxalone, 2 mg/kg; ketamine, 20 mg/kg; and dexmedetomidine, 0.02 mg/kg; n = 10) or ABM (alfaxalone, 2 mg/kg; butorphanol, 2 mg/kg; and midazolam, 1 mg/kg; 9) IM; 1 animal was removed from the study. Atipamezole (I mg/kg) and flumazenil (0.1 mg/kg) were administered 40 minutes after anesthetic induction (defined as loss of the righting reflex) with AKD and ABM, respectively. Heart rate, respiratory rate, oxygen saturation, and reflexes were recorded every 5 minutes. RESULTS The ABM group had significantly longer median times for induction and recovery than the AKD group. Administration of ABM resulted in significantly lower respiratory rates than administration of AKD from time of anesthetic induction to 10 minutes after induction. Respiratory rate significantly decreased in the AKD group from I0 minutes after induction through the end of the anesthetic period but did not change over time in the ABM group. Males had higher respiratory rates in both groups. Loss of the righting reflex was still evident 40 minutes after induction in both groups. In the AKD group, all tested reflexes were absent from I0 to 40 minutes after induction; the ABM group had variable reflexes that recovered within individual animals over time. CONCLUSIONS AND CLINICAL RELEVANCE Both AKD and ABM provided effective immobilization in naked mole-rats, but AKD appeared to provide more consistent and deeper anesthesia, compared with administration of ABM.

A domestic rabbit was diagnosed with a grade III out of IV medial patellar luxation. A trochlear wedge recession and lateral imbrication were performed to replace and stabilize the patella in the trochlear groove. The rabbit recovered well from anesthesia and was held in strict confinement for 8 weeks after surgery to allow the surgical site to heal. At 8 weeks, the rabbit was deemed healed and released to normal activity. At four postoperative months, the patella was stable and maintained appropriate anatomical positioning. At 27 postoperative months, the joint had normal range of motion and the rabbit had normal activity level. This case report details a successful long-term surgical correction of a medially-luxated patella in a pet rabbit.

OBJECTIVE To evaluate the effects of injectable dexmedetomidine-ketamine-midazolam (DKM) and isoflurane inhalation (ISO) anesthetic protocols on selected ocular variables in captive black-tailed prairie dogs (Cynomys ludovicianus; BTPDs). ANIMALS 9 zoo-kept BTPDs. PROCEDURES The BTPDs received dexmedetomidine hydrochloride (0.25 mg/kg, IM), ketamine hydrochloride (40 mg/kg, IM), and midazolam hydrochloride (1.5 mg/kg, IM) or inhalation of isoflurane and oxygen in a randomized complete crossover design (2-day interval between anesthetic episodes). Pupil size, globe position, tear production, and intraocular pressure measurements were recorded at 5, 30, and 45 minutes after induction of anesthesia. For each BTPD, a phenol red thread test was performed in one randomly selected eye and a modified Schirmer tear test I was performed in the other eye. Intraocular pressure was measured by rebound tonometry. RESULTS Compared with findings for the DKM protocol, pupil size was smaller at all time points when the BTPDs underwent the ISO protocol. Globe position remained central during anesthesia with the DKM protocol, whereas it varied among central, ventromedial, and ventrolateral positions during anesthesia with the ISO protocol. Tear production and intraocular pressure decreased significantly over time when the BTPDs underwent either protocol. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that ophthalmic examination findings for anesthetized BTPDs can be influenced by the anesthetic protocol used. The DKM protocol may result in more consistent pupil size and globe position, compared with that achieved by use of the ISO protocol. Tear production and intraocular pressure measurements should be conducted promptly after induction of anesthesia to avoid the effect of anesthetic episode duration on these variables.

A 9-y-old, zoo-housed, male red panda ( Ailurus fulgens) became progressively lethargic and inappetent over a 1-wk period. Physical examination was unremarkable. A complete blood count showed mild normocytic, normochromic, non-regenerative anemia with the presence of trypomastigote organisms, consistent with a Trypanosoma sp. The organism was confirmed later as Trypanosoma cruzi lineage TcI via PCR and genome sequencing. The panda was initially treated supportively; however, its clinical status within 24 h from presentation deteriorated, and euthanasia was elected. Autopsy showed severe systemic T. cruzi infection with the presence of amastigotes in the heart, brain, peripheral nerves, skeletal muscles, tongue, liver, and testes. We used genome sequencing and serology in identifying the agent.

A 40-year-old male double yellow-headed Amazon parrot (Amazona ochrocephala oratrix) was evaluated for a history of chronic recurrent subcutaneous emphysema. Whole-body radiographs and computed tomography imaging revealed significant subcutaneous emphysema in the right cervical area that was suggestive of a chronic ruptured cervical air sac. Surgical exploration of the right cervical area revealed a 4cm defect in the right cervical air sac. The defect was sutured in a double-layer closure first of the thickened air sac tissue, followed by the subcutaneous layer with 5-0 polydioxanone. Following closure of the skin, the remaining air was aspirated from the subcutaneous space. The patient healed without complication, and on last follow-up approximately 5 months postsurgery, there was no sign of recurrence of subcutaneous emphysema, demonstrating successful primary closure of the chronic ruptured cervical air sac. Surgical management of a chronic air sac rupture was successful in resolving recurrent subcutaneous emphysema in an Amazon parrot. The clinical findings, diagnostic approach, anesthesia, surgical technique, and postoperative outcome are described in this clinical report and can aid clinicians presented with cases of chronic, recurrent subcutaneous emphysema that are refractory to other treatments.

In this pilot study, the pharmacokinetics of terbinafine were determined in six apparently healthy red-eared slider turtles (Trachemys scripta elegans) after a single PO administration. Terbinafine suspension (15 mg/kg, once) was administered via gavage tube to all turtles. Blood samples were collected immediately before (time 0) and at 1, 2, 4, 8, 24, and 48 h after drug administration. Plasma terbinafine concentrations were quantified by ultra-performance liquid chromatography-mass spectrometry, and noncompartmental pharmacokinetic analysis was performed. None of the animals showed any adverse responses following terbinafine administration. Mean area under the curve from time 0 to 24 h was 1,213 h × ng/ml (range 319-7,309), mean peak plasma concentration was 201.5 ng/ml (range 45.8-585.3), mean time to maximum plasma concentration was 1.26 h (range 1-4), mean residence time was 7.71 h (range 3.85-14.8), and mean terminal half-life was 5.35 h (range 2.67-9.83). The administration of terbinafine (15 mg/kg, PO) may be appropriate for treatment of select fungal organisms with low minimum inhibitory concentrations in red-eared slider turtles but may require q12h administration even for organisms with low minimum inhibitory concentrations. Multiple-dose studies as well as clinical studies are needed to determine ideal dosages and efficacy.

This study characterized the faecal and caecal microbiota of free-ranging black-tailed prairie dogs in KS, USA, with consideration to their sex and geographic location., Black-tailed prairie dogs (Cynomys ludovicianus) are keystone species within their grassland ecosystems; their population stability affects a multitude of other species. The goals of this study were to explore, describe and compare the bacterial communities in caecal and hard faecal samples from free-ranging black-tailed prairie dogs (n = 36) from KS, USA, using high-throughput sequencing of the V4 region of the 16S rRNA gene and to compare sex and geographic locations. A total of 22 paired faecal and caecal samples were collected post-mortem from free-ranging black-tailed prairie dogs from 5 different geographical locations. The results revealed that the microbiota of both faecal and caecal samples were dominated by the phylum Firmicutes (genera belonging to the Clostridiales order). There was significantly greater richness in faecal compared with caecal samples. There were significant differences between the 5 different geographic regions (P

In this pilot study, the pharmacokinetics of terbinafine were determined in six apparently healthy red-eared slider turtles (

OBJECTIVES To determine the association between signalment, selected haematologic and biochemical parameters and referral centre in pet rabbits with imaging evidence of urolithiasis presented to two veterinary teaching hospitals in North America. MATERIALS AND METHODS The medical record database of two veterinary teaching hospitals was searched from 2009 to 2019 for records of pet rabbits that received both imaging studies and plasma biochemistry profiles. Information regarding signalment, bodyweight, packed cell volume, total solids, and plasma biochemistry profiles was obtained. Univariable and multivariable logistic regression models were performed to identify statistically significant parameters associated with imaging evidence of urolithiasis. RESULTS Of the 324 examined rabbits, 33 (10.2%) had confirmed evidence of urolithiasis on imaging. Increasing plasma calcium and sodium concentrations and referral centre were significantly associated with the presence of urolithiasis on the univariable logistic regression model. However, only plasma calcium concentration and the referral centre demonstrated significant associations on the multivariable logistic regression model. CLINICAL SIGNIFICANCE Results indicate that urolithiasis in pet rabbits that receive imaging is associated with mildly increasing plasma calcium concentration and referral centre. The association with referral centre may indicate there are geographic influences on urolithiasis or on imaging. However, the identified associations have low predictive value for the diagnosis of urolithiasis, indicating the need for additional diagnostic modalities.

In this study, adult intact male and female (n = 10) naked mole rats (Heterocephalus glaber) were anesthetized by using a combination of ketamine (20 mg/kg IM), and alfaxalone (4.0 mg/kg IM). Induction and recovery times were recorded. Vital parameters, including heart rate, respiratory rate, and reflexes, were monitored every 5 min during the anesthetic period. Anesthetic induction was smooth and rapid. Induction time was significantly longer in male rats (median, 325 s; range, 180 to 385 s) than in females (median, 145 s; range, 118 to 180 s). In addition, overall duration of loss of righting reflex was shorter in male mole rats (median, 50 min; range, 36 to 65 min) than females (median, 70 min; range, 60 to 85 min). Males largely had intact withdrawal reflexes, whereas females showed variable loss of both forelimb and hindlimb withdrawal reflexes. Neither recovery time (mean ± 1 SD, 16 ± 13 min) nor vital parameters differed between sexes. None of animals showed any anesthesia-related adverse responses. According to these findings, intramuscular AK is a safe and effective protocol that provides brief, light anesthesia in male naked mole rats and deeper anesthesia in females. We recommend adding analgesics when this AK protocol is used for pain-inducing or invasive procedures, and further studies evaluating higher doses and different combinations are indicated.

The black-tailed prairie dog (Cynomys ludovicianus) is a squirrel-like rodent (Sciuridae) that is used as an animal model for human gallbladder disease, as well as research on several viral and bacterial diseases. Prairie dogs are also prone to develop hepatobiliary diseases that can impair liver function. As a result, monitoring blood urea nitrogen (BUN) concentration or azotemia via low-volume blood samples with immediate test results can be clinically beneficial. In this study, blood samples were collected from captive black-tailed prairie dogs. The BUN analysis was performed in duplicate using a veterinary biochemistry analyzer and commercial reagent test strips. The indication of azotemia by the reagent test strips, when compared to plasma BUN results of prairie dogs with and without confirmed azotemia, had a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 95%, 71%, and 100%, respectively. Based on the results of this study, the reagent test strips provided a moderately accurate method of estimating the BUN concentration and high accuracy reporting both normal and azotemic black-tailed prairie dogs. Continued investigation into alternative point-of-care testing for prairie dogs is warranted, but standard blood chemistry analyzers should still be considered the most accurate method of BUN testing and azotemia determination for this species.

OBJECTIVE To investigate effects of storage duration and temperature on biochemical analytes in plasma from red-eared sliders (Trachemys scripta elegans). ANIMALS 8 red-eared sliders. PROCEDURES Blood samples were collected. Plasma was harvested and analyzed at room temperature (approx 23°C; time = 1 hour) and then fractioned into 0.1-mL aliquots that were stored at room temperature or were refrigerated (4°C) or frozen (−20°C). Biochemical analysis of stored samples was performed at 4 (room temperature), 8 (4°C), 24 (4°C), 48 (4° and −20°C), and 72 (−20°C) hours and at 7 days (−20°C). For each time point for each storage temperature, bias was calculated by subtracting values from the value obtained at 1 hour. Bias was modeled by use of a linear mixed model. RESULTS Storage temperature had a significant effect on several plasma biochemical analytes. In general, aspartate aminotransferase activity and uric acid, total protein, and potassium concentrations increased after storage at 4° and −20°C. Differences in values after storage were mostly within the acceptable range for allowable total error, except for calcium and potassium concentrations for samples stored at −20°C. Both storage temperatures increased variability of measurement results. Results for samples stored at room temperature for 4 hours did not differ significantly from values at 1 hour. Results differed significantly between refrigerated and frozen samples stored for 48 hours. CONCLUSIONS AND CLINICAL RELEVANCE Short-term storage conditions influenced results for some biochemical analytes. These effects should be considered when performing biochemical analyses of plasma samples obtained from red-eared sliders.

CASE DESCRIPTION A 2-year-old male bearded dragon (Pogona vitticeps) was evaluated because of a traumatic mandibular fracture. CLINICAL FINDINGS An open comminuted fracture of the rostral aspect of the right mandible was evident, with a fragment of bone exposed and dorsally displaced. Whole-body radiography revealed no evidence of additional injury. Other findings were unremarkable, except for moderate anemia (PCV, 19%). TREATMENT AND OUTCOME The fracture fragments were stabilized with 2 crossed 36-gauge interfragmentary wire loops. An external fixator device was fashioned from four 25-gauge needles inserted at alternating angles through the fracture fragments; plastic IV fluid line tubing filled with dental acrylic was used as a connecting bar. One day after surgery, the lizard had regained its typical activity level and appetite. Body weight was measured and the external fixator was inspected 1 week after surgery and monthly thereafter. Three months after initial injury, the fracture was stable, radiography revealed bony callus formation at the fracture site, and the external fixator was removed. Recheck radiography performed 5.5 months after initial injury revealed complete osseous union of the fracture fragments, and the interfragmentary wires were removed. CLINICAL RELEVANCE Surgical management of the traumatic comminuted mandibular fracture in this bearded dragon by means of a combination of internal and external fixation resulted in complete healing of the mandible and restoration of function. Management of this complicated fracture was achieved with the aid of readily available and inexpensive supplies in a clinical setting, which may be useful to other clinicians in the management of similar cases.

A 6-year-old, 1.9-kg female spayed lop-eared European rabbit ( Oryctolagus cuniculus ) was evaluated for acute hematuria and inappropriate urination and defecation of less than 12-hours duration. Abdominal ultrasonography revealed a suspected bladder mass. Ultrasound-guided cystocentesis was performed and the results of the urinalysis were unremarkable. An abdominal exploratory surgery revealed a nodular cystic mass at the cranial ventral apex of the bladder wall which measured 0.5cm x 0.5cm. Based on histopathologic evaluation, excision of the neoplasm was complete and the mass was diagnosed as transitional cell carcinoma (TCC) of the bladder. This case suggests that urinary bladder TCC should be considered in pet rabbits presenting with similar clinical signs.

Myiasis is a major disease condition in human and veterinary medicine. Domestic, free-ranging, and zoo-housed animals can be severely affected by myiasis. Depending on case severity, multiple treatment episodes may be indicated and can lead to recurrent capturing, handling stress, and anesthetics, all of which increase the risk of adverse responses (including death) individually and also in the herd. As an insecticide, ivermectin is often used for larval control. A total of 28 individual myiasis cases were retrospectively evaluated, out of which 11 cases were also treated using an ivermectin sustained-release varnish (SRV). The clinical outcome of all cases was assessed and the results suggest that the use of a topical ivermectin SRV (with or without concurrent injectable ivermectin) can reduce handling and treatments, has no adverse effects, and has minimal recurrence of the disease when compared with cases treated without it.

Hepatocellular carcinomas are the most commonly reported neoplasm of black-tailed prairie dogs (Cynomys ludovicianus). In several other closely related Sciuridae species, infection with species-specific hepadnaviruses is associated with the development of these tumours, but such a hepadnavirus has not yet been identified in any prairie dog species, although its presence has been hypothesized previously. An adult prairie dog was humanely destroyed due to progressive illness and the identification of a cranial abdominal mass that was determined on histopathology to be a hepatocellular carcinoma. Deep sequencing of the tumour tissue identified the presence of a hepadnavirus, similar in its genetic structure to woodchuck hepatitis virus. Electron microscopy showed the presence of viral particles similar in structure to other hepadnaviral particles. This report suggests that a hepadnavirus may be associated with the development of hepatocellular carcinomas in the prairie dog.

In both human and veterinary medicine, urine specific gravity (USG) is commonly measured by refractometry to indirectly reflect the osmolality of urine to thereby evaluate the kidney's ability to concentrate or dilute urine according to physiologic need and certain disease conditions. However, for accurate interpretation of the significance of any value, knowledge of the expected USG for the healthy species in question is required. It is generally believed that fruit bats, and Egyptian fruit bats (Rousettus aegyptiacus) in particular, are unable to highly concentrate their urine. In this study, the USG was determined using a handheld urine refractometer in 43 free-living Egyptian fruit bats of both sexes. The calculated nonparametric 90% confidence interval for Egyptian fruit bats in this study was 1.006–1.050, with no association with capture site, sex, weight, or packed cell volume and total solids. Results suggest that free-living Egyptian fruit bats are able to highly concentrate their urine.

Isoflurane gas anesthesia is often used for immobilization of prairie dogs in field studies, laboratory research, and veterinary clinical purposes. The goals of this prospective study were to evaluate the effects of sternal versus right lateral recumbency on trends of selected physiologic parameters during isoflurane anesthesia in black-tailed prairie dogs ( Cynomys ludovicianus ). Fourteen adult, zoo-housed black-tailed prairie dogs were tested during the study. Animals were anesthetized using isoflurane and randomly placed in either sternal or right lateral recumbency to evaluate changes in trends of physiologic parameters, measured selectively every 30 min throughout a 60-min anesthesia period. Results were analyzed using linear mixed modeling. Right lateral recumbency resulted in a decrease in anion gap of about 4.6 mEq/L (95% confidence interval [95% CI]: 3.1-6.0, P0.001), whereas sternal recumbency resulted in a lower decrease of 2.1 mEq/L (95% CI: 0.7-3.6, P = 0.02). However, the absolute values at the beginning and at the end of the anesthesia time were not significantly different between the right lateral and sternal recumbency (all P0.57). Body position did not have any effect on any other variables, and most of the observed physiologic changes were due to the duration of anesthesia. Our results indicate no significant effect on trends of selected physiologic parameters between sternal recumbency and right lateral recumbency during 1 hr of isoflurane anesthesia in black-tailed prairie dogs.

A female North American porcupine ( Erethizon dorsatum ) was evaluated for a unilateral pedal crusting and alopecic dermatopathy. Fungal culture and histopathology testing revealed Microsporum gypseum dermatophytosis. Treatment with topical miconazole was initiated and then discontinued after 9 days and changed to oral terbinafine. Twenty-eight days after initial examination, clinical signs were improving, and fungal cultures of the front foot, muzzle, and noninfected area along the dorsum were negative for M. gypseum. Visual exams were conducted on a regular basis. Eighty-three days after initial evaluation, clinical signs had completely resolved and repeat fungal cultures were negative. One of the animal's keepers was suspected to have acquired a dermal fungal infection 3 days after contact with this porcupine, and lesions had resolved after treatment with topical ketoconazole. To the authors' knowledge, this is the first report of M. gypseum diagnosed and treated in a captive North American porcupine. Veterinary staff and zookeepers should be aware of this potentially zoonotic infection.

Green iguanas with gastrointestinal, hepatic, and reproductive disease may present to the veterinary clinic displaying non-specific clinical signs, necessitating a thorough clinical evaluation. A 15-year-old intact female green iguana (Iguana iguana) presented with a 3-week history of decreased appetite and coelomic distension. A complete blood count and plasma biochemistry analysis showed anaemia and leukopenia with an absolute lymphopenia; decreased sodium and chloride concentrations; and increased phosphorus, potassium, aspartate transaminase, and uric acid concentrations. Whole body radiographs revealed a large, round, homogenous soft tissue opacity structure in the coelom. Ultrasonography confirmed a thin-walled cystic structure located in the cranial coelomic cavity. An exploratory coeliotomy revealed a large hepatic cyst, diffusely abnormal hepatic parenchyma, and cysts on the right ovary. The right ovary and hepatic cyst were surgically excised, however the iguana died under general anesthesia. Histopathology provided a diagnosis of multifocal cholangiocarcinoma and ovarian papillary adenocarcinoma. This case report alerts the clinician to the clinical presentation, anaesthetic protocol, surgical intervention, and challenges associated with the diagnosis of a large cystic cholangiocarcinoma in a pet green iguana.

This study was conducted to determine plasma thyroxine (T4) concentrations in zoo-kept black-tailed prairie dogs (Cynomys ludovicianus). Thirty-one healthy prairie dogs of both sexes were studied as part of their annual clinical health evaluation, performed under general isoflurane anesthesia. Each animal underwent a complete physical examination, complete blood count, plasma biochemistry, and venous blood gas analysis. Heparinized venous blood samples were collected individually and processed for plasma T4 analysis using a veterinary biochemistry analyzer. The median plasma T4 concentration for the prairie dogs in this study was 4.1 μg/dl (minimum = 0.6 μg/dl; maximum = 8.0 μg/dl). The mean ± standard deviation plasma T4 concentration was 4.49 ± 2.39 μg/dl. No significant differences were found for varying ages, sexes, weights, or housing systems. The data presented in this report can promote better physiologic understanding and improve clinical management of this rodent species.

To evaluate the utility of commercially available reagent test strips for estimation of BUN concentration and detection of azotemia in pet rabbits (65 blood samples from 53 rabbits and 71 blood samples from 50 ferrets of various health statuses.BUN concentrations were measured with a clinical laboratory biochemical analyzer and estimated with a reagent test strip. Results obtained with both methods were assigned to a BUN category (range, 1 to 4; higher categories corresponded to higher BUN concentrations). Samples with a biochemical analyzer BUN concentration ≥ 27 mg/dL (rabbits) or ≥ 41 mg/dL (ferrets) were considered azotemic. A test strip BUN category of 3 or 4 (rabbits) or 4 (ferrets) was considered positive for azotemia.Test strip and biochemical analyzer BUN categories were concordant for 46 of 65 (71%) rabbit blood samples and 58 of 71 (82%) ferret blood samples. Sensitivity, specificity, and accuracy of the test strips for detection of azotemia were 92%, 79%, and 82%, respectively, for rabbit blood samples and 80%, 100%, and 96%, respectively, for ferret blood samples.Test strips provided reasonable estimates of BUN concentration but, for rabbits, were more appropriate for ruling out than for ruling in azotemia because of false-positive test strip results. False-negative test strip results for azotemia were more of a concern for ferrets than rabbits. Testing with a biochemical analyzer remains the gold standard for measurement of BUN concentration and detection of azotemia in rabbits and ferrets.