1. Dysregulation of NEUROG2 plays a key role in focal cortical dysplasia
- Author
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Avansini, Simoni H., Torres, Fábio R., Vieira, André S., Dogini, Danyella B., Rogerio, Fabio, Coan, Ana C., Morita, Marcia E., Guerreiro, Marilisa M., Yasuda, Clarissa L., Secolin, Rodrigo, Carvalho, Benilton S., Borges, Murilo G., Almeida, Vanessa S., Araújo, Patrícia A. O. R., Queiroz, Luciano, Cendes, Fernando, and Lopes‐Cendes, Iscia
- Subjects
Adult ,Male ,Neurons ,rho GTP-Binding Proteins ,Drug Resistant Epilepsy ,Epilepsy ,Adolescent ,TOR Serine-Threonine Kinases ,Infant ,Nerve Tissue Proteins ,Focal Dermal Hypoplasia ,Malformations of Cortical Development ,Young Adult ,Child, Preschool ,embryonic structures ,Basic Helix-Loop-Helix Transcription Factors ,Humans ,Female ,Child ,Research Articles ,Research Article ,Transcription Factors - Abstract
Objective Focal cortical dysplasias (FCDs) are an important cause of drug‐resistant epilepsy. In this work, we aimed to investigate whether abnormal gene regulation, mediated by microRNA, could be involved in FCD type II. Methods We used total RNA from the brain tissue of 16 patients with FCD type II and 28 controls. MicroRNA expression was initially assessed by microarray. Quantitative polymerase chain reaction, in situ hybridization, luciferase reporter assays, and deep sequencing for genes in the mTOR pathway were performed to validate and further explore our initial study. Results hsa‐let‐7f (p = 0.039), hsa‐miR‐31 (p = 0.0078), and hsa‐miR34a (p = 0.021) were downregulated in FCD type II, whereas a transcription factor involved in neuronal and glial fate specification, NEUROG2 (p
- Published
- 2018