Author: "Christodoulou A." / Database: OpenAIRE - Searchworks@Jio Institute Digital Library Search Results

10,570 results on '"Christodoulou A."'

1. Current knowledge and contemporary management of non-A non-B aortic dissections

Author: Konstantinos C Christodoulou, Dimos Karangelis, Gioultzan Memet Efenti, Panagiotis Sdrevanos, Jennifer R Browning, Fotis Konstantinou, Efstratios Georgakarakos, Fotios A Mitropoulos, and Dimitrios Mikroulis
Subjects: Cardiology and Cardiovascular Medicine
Published: 2023

2. Adult Cancer-Related Emergency Department Utilization: An Analysis of Trends and Outcomes From Emergency Departments in Maryland and New York

Author: Ilias Christodoulou, Benjamin Ukert, Michael A. Vavuranakis, Hye-Chung Kum, and Theodoros V. Giannouchos
Subjects: Oncology, Oncology (nursing), Health Policy
Abstract: PURPOSE To explore emergency department (ED) visits by adults with cancer and to estimate associations between inpatient admissions through the ED and mortality with sociodemographic and clinical factors within this cohort. METHODS We conducted a retrospective, pooled, cross-sectional analysis of the Healthcare Cost and Utilization State Emergency Department Databases and State Inpatient Databases for Maryland and New York from January 2013 to December 2017. We examined inpatient admissions through the ED and mortality using frequencies. Among patients with cancer, multivariable regressions were used to estimate sociodemographic and clinical factors associated with inpatient admissions and outpatient ED and inpatient mortality overall. RESULTS Among 22.7 million adult ED users, 1.3 million (5.7%) had at least one cancer-related diagnosis. ED visit rates per 100,000 population increased annually throughout the study period for patients with cancer and were 9.9% higher in 2017 compared with 2013 (2013: 303.5; 2017: 333.6). Having at least one inpatient admission (68.7% v 20.5%; P < .001) and inpatient or ED mortality (6.5% v 1.0%; P < .001) were higher among ED users with cancer compared with those without. Among patients with cancer, being uninsured (adjusted odds ratio, 0.52; 95% CI, 0.44 to 0.62) compared with having Medicare coverage and non-Hispanic Black (adjusted odds ratio, 0.86; 95% CI, 0.80 to 0.92) compared with non-Hispanic White were associated with decreased odds of inpatient admissions. In contrast, patients with cancer without health insurance, non-Hispanic Black patients, and residents of nonlarge metropolitan areas and of areas with lower household incomes had increased odds of mortality. CONCLUSION High inpatient admissions through the ED and mortality among adult patients with cancer, coupled with an increase in cancer-related ED visit rates and observed disparities in outcomes, highlight the need to improve access to oncologic services to contain ED use and improve care for patients with cancer.
Published: 2023

3. A novel conceptual framework for the functionality of the glymphatic system

Author: Kyriaki Astara, Christina Pournara, Edoardo Rosario de Natale, Heather Wilson, George D. Vavougios, Andreas S. Lappas, Marios Politis, and Nikos G. Christodoulou
Subjects: Physiology, General Neuroscience
Abstract: The glymphatic system is responsible for the clearance of the potentially harmful metabolic waste of the central nervous system. The prevalent theory is that the cerebrospinal fluid (CSF) circulates in the perivascular space (PVS) and through the astrocytes’ aquaporin-4 channels (AQ-4), and it is then drained by the lymphatic vessels after mixing with interstitial fluid (ISF). However, there is little evidence supporting this hypothesis. A deeper understanding of the physiology of the glymphatic system could transform the way we understand neuropathology and our approach to treating neurological and neuropsychiatric disorders. In this review, we introduce a new conceptual framework for the functionality of the glymphatic system, offering new directions for future research. We propose that CSF and ISF exchange flow depends on arterial pulsation, respiration, posture, and sleep. PVS changes due to disrupted cerebral autoregulation, alternations of intrathoracic pressure, venous flow, and body position can also influence the glymphatic flow. The role of respiration remains controversial due to the variety of parameters that interfere with glymphatic functionality. Slow-wave sleep is important for glymphatic clearance due to neuronal electromagnetic synchronization and expansion of the interstitial space. Therefore, sleep and vascular disorders, as well as aging, may hinder glymphatic flow and induce a noxious milieu of susceptibility to neurodegenerative disorders because of metabolic waste accumulation. We lastly introduce a new idea postulating that electromagnetic induction may constitute one of the propelling forces for the convectional current and mixing of CSF and ISF.
Published: 2023

4. TEFM variants impair mitochondrial transcription causing childhood-onset neurological disease

Author: Lindsey Van Haute, Emily O’Connor, Héctor Díaz-Maldonado, Benjamin Munro, Kiran Polavarapu, Daniella H. Hock, Gautham Arunachal, Alkyoni Athanasiou-Fragkouli, Mainak Bardhan, Magalie Barth, Dominique Bonneau, Nicola Brunetti-Pierri, Gerarda Cappuccio, Nikeisha J. Caruana, Natalia Dominik, Himanshu Goel, Guy Helman, Henry Houlden, Guy Lenaers, Karine Mention, David Murphy, Bevinahalli Nandeesh, Catarina Olimpio, Christopher A. Powell, Veeramani Preethish-Kumar, Vincent Procaccio, Rocio Rius, Pedro Rebelo-Guiomar, Cas Simons, Seena Vengalil, Maha S. Zaki, Alban Ziegler, David R. Thorburn, David A. Stroud, Reza Maroofian, John Christodoulou, Claes Gustafsson, Atchayaram Nalini, Hanns Lochmüller, Michal Minczuk, Rita Horvath, Van Haute, Lindsey [0000-0001-7809-1473], Polavarapu, Kiran [0000-0002-8879-6001], Hock, Daniella H [0000-0002-6940-4420], Bardhan, Mainak [0000-0002-4106-409X], Brunetti-Pierri, Nicola [0000-0002-6895-8819], Caruana, Nikeisha J [0000-0002-0817-1686], Helman, Guy [0000-0002-4784-7423], Houlden, Henry [0000-0002-2866-7777], Lenaers, Guy [0000-0003-2736-3349], Rius, Rocio [0000-0002-9871-3126], Rebelo-Guiomar, Pedro [0000-0002-5060-7519], Simons, Cas [0000-0003-3147-8042], Vengalil, Seena [0000-0002-0629-9221], Zaki, Maha S [0000-0001-7840-0002], Thorburn, David R [0000-0002-7725-9470], Stroud, David A [0000-0002-2048-3383], Christodoulou, John [0000-0002-8431-0641], Gustafsson, Claes [0000-0003-3531-8468], Minczuk, Michal [0000-0001-8242-1420], Horvath, Rita [0000-0002-9841-170X], Apollo - University of Cambridge Repository, Van Haute, Lindsey, O'Connor, Emily, Díaz-Maldonado, Héctor, Munro, Benjamin, Polavarapu, Kiran, Hock, Daniella H, Arunachal, Gautham, Athanasiou-Fragkouli, Alkyoni, Bardhan, Mainak, Barth, Magalie, Bonneau, Dominique, Brunetti-Pierri, Nicola, Cappuccio, Gerarda, Caruana, Nikeisha J, Dominik, Natalia, Goel, Himanshu, Helman, Guy, Houlden, Henry, Lenaers, Guy, Mention, Karine, Murphy, David, Nandeesh, Bevinahalli, Olimpio, Catarina, Powell, Christopher A, Preethish-Kumar, Veeramani, Procaccio, Vincent, Rius, Rocio, Rebelo-Guiomar, Pedro, Simons, Ca, Vengalil, Seena, Zaki, Maha S, Ziegler, Alban, Thorburn, David R, Stroud, David A, Maroofian, Reza, Christodoulou, John, Gustafsson, Clae, Nalini, Atchayaram, Lochmüller, Hann, Minczuk, Michal, and Horvath, Rita
Subjects: Transcription, Genetic, RNA, Mitochondrial, General Physics and Astronomy, 38/90, 13/106, 692/1807/1693, 14, DNA, Mitochondrial, General Biochemistry, Genetics and Molecular Biology, 38, 38/91, 82/80, Mitochondrial Proteins, 38/1, 692/420/2489/144, 692/617/375/374, 38/23, 38/22, Animals, Humans, Child, Zebrafish, 64, Multidisciplinary, 64/116, 692/308/2056, article, General Chemistry, 13/51, Mutation, 38/77, 692/700/139/422, Transcription Factors
Abstract: Funder: EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013)); doi: https://doi.org/10.13039/100011199; Grant(s): 309548, Funder: Lily Mae Foundation; doi: https://doi.org/10.13039/100012336, Mutations in the mitochondrial or nuclear genomes are associated with a diverse group of human disorders characterized by impaired mitochondrial respiration. Within this group, an increasing number of mutations have been identified in nuclear genes involved in mitochondrial RNA biology. The TEFM gene encodes the mitochondrial transcription elongation factor responsible for enhancing the processivity of mitochondrial RNA polymerase, POLRMT. We report for the first time that TEFM variants are associated with mitochondrial respiratory chain deficiency and a wide range of clinical presentations including mitochondrial myopathy with a treatable neuromuscular transmission defect. Mechanistically, we show muscle and primary fibroblasts from the affected individuals have reduced levels of promoter distal mitochondrial RNA transcripts. Finally, tefm knockdown in zebrafish embryos resulted in neuromuscular junction abnormalities and abnormal mitochondrial function, strengthening the genotype-phenotype correlation. Our study highlights that TEFM regulates mitochondrial transcription elongation and its defect results in variable, tissue-specific neurological and neuromuscular symptoms.
Published: 2023
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5. Endoscopic luminal stenting: Current applications and future perspectives

Author: Miltiadis Moutzoukis, Konstantinos Argyriou, Andreas Kapsoritakis, and Dimitrios Christodoulou
Subjects: Critical Care Nursing, Pediatrics
Published: 2023

6. Inherited and acquired errors of type I interferon immunity govern susceptibility to COVID-19 and multisystem inflammatory syndrome in children

Author: Giorgia Bucciol, Isabelle Meyts, Laurent Abel, Salah Al-Muhsen, Alessandro Aiuti, Fahd Al-Mulla, Evangelos Andreakos, Novelli Antonio, Andrés A. Arias, Sophie Trouillet-Assant, Alexandre Belot, Catherine M. Biggs, Ahmed A. Bousfiha, Alex Bolze, Alessandro Borghesi, Petter Brodin, John Christodoulou, Aurélie Cobat, Antonio Condino-Neto, Stefan Constantinescu, Clifton L. Dalgard, Sara Espinosa-Padilla, Jacques Fellay, Carlos Flores, José Luis Franco, Antoine Froidure, Guy Gorochov, Filomeen Haerynck, Rabih Halwani, Elena W.Y. Hsieh, Yuval Itan, Kai Kisand, Yu-Lung Lau, Davood Mansouri, Trine H. Mogensen, Lisa F.P. Ng, Luigi D. Notarangelo, Giuseppe Novelli, Satoshi Okada, Tayfun Ozcelik, Rebeca Perez de Diego, Carolina Prando, Aurora Pujol, Lluis Quintana-Murci, Laurent Renia, Igor Resnick, Lucie Roussel, Carlos Rodríguez-Gallego, Vanessa Sancho-Shimizu, Mohammed Shahrooei, Pere Soler-Palacín, András N. Spaan, Ivan Tancevski, Stuart G. Tangye, Ahmad Abou Tayoun, Şehime Gülsün Temel, Pierre Tiberghien, Jordi Perez Tur, Stuart E. Turvey, Furkan Uddin, Mohammed J. Uddin, Mateus Vidigal, Donald C. Vinh, Mayana Zatz, Keisuke Okamoto, David S. Perlin, Graziano Pesole, Christian Thorball, Diederik van de Beek, Roger Colobran, Joost Wauters, Shen-Ying Zhang, Qian Zhang, Helen C. Su, and Jean-Laurent Casanova
Subjects: Settore MED/03, SARS-CoV-2, Immunology, Immunology and Allergy, COVID-19, type I interferon, multisystem inflammatory syndrome in children
Abstract: Since the beginning of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/coronavirus disease 2019 (COVID-19) pandemic, global sequencing efforts have led in the field of inborn errors of immunity, and inspired particularly by previous research on life-threatening influenza, they have revealed that known and novel inborn errors affecting type I interferon immunity underlie critical COVID-19 in up to 5% of cases. In addition, neutralizing autoantibodies against type I interferons have been identified in up to 20% of patients with critical COVID-19 who are older than 80 years and 20% of fatal cases, with a higher prevalence in men and individuals older than 70 years. Also, inborn errors impairing regulation of type I interferon responses and RNA degradation have been found as causes of multisystem inflammatory syndrome in children, a life-threatening hyperinflammatory condition complicating otherwise mild initial SARS-CoV-2 infection in children and young adults. Better understanding of these immunologic mechanisms can aid in designing treatments for severe COVID-19, multisystem inflammatory syndrome in children, long COVID, and neuro-COVID.
Published: 2023

7. A cryptic pathogenic <scp> NDUFV1 </scp> variant identified by <scp>RNA</scp> ‐seq in a patient with normal complex I activity in muscle and transient magnetic resonance imaging changes

Author: Sharmila Kiss, John Christodoulou, David R. Thorburn, Jeremy L. Freeman, Andrew J. Kornberg, Simone Mandelstam, Alison G. Compton, Beryl Cummings, Lynn Pais, Joy Yaplito‐Lee, and Susan M. White
Subjects: Genetics, Genetics (clinical)
Published: 2023

8. Australian Genomics: Outcomes of a 5-year national program to accelerate the integration of genomics in healthcare

Author: Zornitza Stark, Tiffany Boughtwood, Matilda Haas, Jeffrey Braithwaite, Clara L. Gaff, Ilias Goranitis, Amanda B. Spurdle, David P. Hansen, Oliver Hofmann, Nigel Laing, Sylvia Metcalfe, Ainsley J. Newson, Hamish S. Scott, Natalie Thorne, Robyn L. Ward, Marcel E. Dinger, Stephanie Best, Janet C. Long, Sean M. Grimmond, John Pearson, Nicola Waddell, Christopher P. Barnett, Matthew Cook, Michael Field, David Fielding, Stephen B. Fox, Jozef Gecz, Adam Jaffe, Richard J. Leventer, Paul J. Lockhart, Sebastian Lunke, Andrew J. Mallett, Julie McGaughran, Linda Mileshkin, Katia Nones, Tony Roscioli, Ingrid E. Scheffer, Christopher Semsarian, Cas Simons, David M. Thomas, David R. Thorburn, Richard Tothill, Deborah White, Sally Dunwoodie, Peter T. Simpson, Peta Phillips, Marie-Jo Brion, Keri Finlay, Michael CJ. Quinn, Tessa Mattiske, Emma Tudini, Kirsten Boggs, Sean Murray, Kathy Wells, John Cannings, Andrew H. Sinclair, John Christodoulou, Kathryn N. North, Stark, Zornitza, Boughtwood, Tiffany, Haas, Matilda, Braithwaite, Jeffrey, Scott, Hamish S, and North, Kathryn N
Subjects: Genetics, Genetics (clinical)
Abstract: Refereed/Peer-reviewed Australian Genomics is a national collaborative partnership of more than 100 organizations piloting a whole-of-system approach to integrating genomics into healthcare, based on federation principles. In the first five years of operation, Australian Genomics has evaluated the outcomes of genomic testing in more than 5,200 individuals across 19 rare disease and cancer flagship studies. Comprehensive analyses of the health economic, policy, ethical, legal, implementation and workforce implications of incorporating genomics in the Australian context have informed evidence-based change in policy and practice, resulting in national government funding and equity of access for a range of genomic tests. Simultaneously, Australian Genomics has built national skills, infrastructure, policy, and data resources to enable effective data sharing to drive discovery research and support improvements in clinical genomic delivery.
Published: 2023

9. Serving decision makers and their employees simultaneously: Adopting a balanced approach

Author: Rick Laguerre, Jennifer Bragger, Edileide Cavalcanti, Christina Christodoulou, Sara Stavely, and Morgan Russell
Subjects: Social Psychology, Applied Psychology
Published: 2023

10. Somatic symptoms associated with mental distress during the COVID-19 pandemic: a systematic review

Author: Andreas Theocharis, Victor Antonopoulos, and Nikos G Christodoulou
Subjects: Psychiatry and Mental health
Abstract: Objective The primary objective of this article was to systematically review the prevalence of somatic symptoms in patients, healthcare workers and general population during the COVID-19 pandemic. The secondary objectives were to record the association between somatic symptoms and indices of mental distress and to record the qualitative characteristics of such somatic complaints. Methods Following the PRISMA methodology we systematically searched PubMed, Scopus and Google Scholar for primary research reporting on somatic symptoms in association with mental distress. We specifically sought papers reporting on bodily distress disorder, somatic symptoms disorder or medically unexplained symptoms. Results 1569 papers were retrieved, which yielded 36 relevant studies after screening. 15 studies referred to healthcare workers, 13 to general population, 5 to COVID-19 patients, 2 compared medical with non-medical professionals and 1 was studying COVID-19 patients some of which were also healthcare workers. The reported prevalence of somatic symptoms ranged from 7.4% to 67.8%. Commonly seen symptoms were shortness of breath, dyspnea, palpitations and pain in arms, legs and joints. Conclusions Our systematic review suggests that somatic symptoms are very frequent during the pandemic and are often linked to mental distress. All three population groups are affected.
Published: 2023

11. The phenotypic spectrum of pathogenic ATP1A1 variants expands: the novel p.P600R substitution causes demyelinating Charcot–Marie–Tooth disease

Author: Feride Cinarli Yuksel, Paschalis Nicolaou, Kerri Spontarelli, Maike F. Dohrn, Adriana P. Rebelo, Pantelitsa Koutsou, Anthi Georghiou, Pablo Artigas, Stephan L. Züchner, Kleopas A. Kleopa, and Kyproula Christodoulou
Subjects: Neurology, Neurology (clinical)
Abstract: Background Charcot–Marie–Tooth disease (CMT) is a genetically and clinically heterogeneous group of inherited neuropathies. Monoallelic pathogenic variants in ATP1A1 were associated with axonal and intermediate CMT. ATP1A1 encodes for the catalytic α1 subunit of the Na+/ K+ ATPase. Besides neuropathy, other associated phenotypes are spastic paraplegia, intellectual disability, and renal hypomagnesemia. We hereby report the first demyelinating CMT case due to a novel ATP1A1 variant. Methods Whole-exome sequencing on the patient’s genomic DNA and Sanger sequencing to validate and confirm the segregation of the identified p.P600R ATP1A1 variation were performed. To evaluate functional effects, blood-derived mRNA and protein levels of ATP1A1 and the auxiliary β1 subunit encoded by ATP1B1 were investigated. The ouabain-survival assay was performed in transfected HEK cells to assess cell viability, and two-electrode voltage clamp studies were performed in Xenopus oocytes. Results The variant was absent in the local and global control datasets, falls within a highly conserved protein position, and is in a missense-constrained region. The expression levels of ATP1A1 and ATP1B1 were significantly reduced in the patient compared to healthy controls. Electrophysiology indicated that ATP1A1p.P600R injected Xenopus oocytes have reduced Na+/ K+ ATPase function. Moreover, HEK cells transfected with a construct encoding ATP1A1p.P600R harbouring variants that confers ouabain insensitivity displayed a significant decrease in cell viability after ouabain treatment compared to the wild type, further supporting the pathogenicity of this variant. Conclusion Our results further confirm the causative role of ATP1A1 in peripheral neuropathy and broaden the mutational and phenotypic spectrum of ATP1A1-associated CMT.
Published: 2023

12. Safety of COVID-19 vaccines in multiple sclerosis: A systematic review and meta-analysis

Author: Maria-Ioanna Stefanou, Lina Palaiodimou, Aikaterini Theodorou, Maria Veatriki Christodoulou, John S Tzartos, Dimitrios Tzanetakos, Dimitrios Kitsos, Maria Chondrogianni, Vasiliki Zouvelou, Efthimios Dardiotis, Elias Tzavellas, Ekaterini Syrigou, Vassiliki Benetou, George P Paraskevas, Sotirios Tsiodras, Georgios Tsivgoulis, and Sotirios Giannopoulos
Subjects: Neurology, Neurology (clinical)
Abstract: Background Data are sparse regarding the safety of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines in patients with multiple sclerosis (MS). Objective To estimate (1) the pooled proportion of MS patients experiencing relapse among vaccine recipients; (2) the rate of transient neurological worsening, adverse events, and serious adverse events; (3) the previous outcomes of interest for different SARS-CoV-2 vaccine types. Methods Systematic review and meta-analysis of pharmacovigilance registries and observational studies. Results Nineteen observational studies comprising 14,755 MS patients who received 23,088 doses of COVID-19 vaccines were included. Mean age was 43.3 years (95% confidence interval (CI): 40–46.6); relapsing-remitting, secondary-progressive, primary-progressive MS and clinically isolated syndrome were diagnosed in 82.6% (95% CI: 73.9–89.8), 12.6% (95% CI: 6.3–20.8), 6.7% (95% CI: 4.2–9.9), and 2.9% (95% CI: 1–5.9) of cases, respectively. The pooled proportion of MS patients experiencing relapse at a mean time interval of 20 days (95% CI: 12–28.2) from vaccination was 1.9% (95% CI: 1.3%–2.6%; I2 = 78%), with the relapse risk being independent of the type of administered SARS-CoV-2-vaccine ( p for subgroup differences = 0.7 for messenger RNA (mRNA), inactivated virus, and adenovector-based vaccines). After vaccination, transient neurological worsening was observed in 4.8% (95% CI: 2.3%–8.1%) of patients. Adverse events and serious adverse events were reported in 52.8% (95% CI: 46.7%–58.8%) and 0.1% (95% CI: 0%–0.2%) of vaccinations, respectively. Conclusion COVID-19 vaccination does not appear to increase the risk of relapse and serious adverse events in MS. Weighted against the risks of SARS-CoV-2-related complications and MS exacerbations, these safety data provide compelling pro-vaccination arguments for MS patients.
Published: 2023

13. Sleep quality of medical students and relationships with academic performances

Author: N, Christodoulou, J, Maruani, M-P, d'Ortho, M, Lejoyeux, and P A, Geoffroy
Subjects: Psychiatry and Mental health, Arts and Humanities (miscellaneous)
Abstract: Sleep health is a major public health concern because of its correlation with physical and mental health, and it may be particularly altered in medical students. This study aims: i) to examine the sleep characteristics of French medical students and their knowledge about basic sleep hygiene rules and; ii) to examine the correlations between sleep quality and academic performances, as well as between sleep quality and sleep knowledge.Students from 4th, 5th and 6th years of medicine, of the Faculty of Paris Diderot, voluntarily responded to an online questionnaire including PSQI and multiple-choice quizzes (MCQ) about basic sleep hygiene rules.From the 177 participants, 49.7% had a poor sleep (PSQI5). Regarding sleep latency, 44.6% needed30min to fall asleep at least once a week, 26.5% slept 6 hours or less by night, 42.4% of them qualified their sleep quality as bad or very bad. A serious lack of knowledge about basic sleep hygiene rules was observed, with an average score at the MCQ of 6.61/10, and only 31% of medical students were aware of basic good sleep habits. Significant correlations were observed between sleep efficiency and all academic mean scores (both regarding the morning, afternoon, and pooled mean scores), and between sleep disturbances and the morning mean score.French medical students have a poor sleep quality, correlating with academic performances, and present a poor knowledge of basic sleep rules. These findings are a call to improve medical training schedules and to develop prevention and training programs.
Published: 2023

14. Magnetic Shielding for Electric Car Power Cables

Author: T. Damatopoulou, S. Angelopoulos, C. Christodoulou, I. Gonos, A. Kladas, and E. Hristoforou
Subjects: Electrical and Electronic Engineering, Electronic, Optical and Magnetic Materials
Published: 2023

15. Affinity-enhanced RNA-binding domains as tools to understand RNA recognition

Author: Chaves-Arquero, Belén, Collins, Katherine M, Abis, Giancarlo, Kelly, Geoff, Christodoulou, Evangelos, Taylor, Ian A, Ramos, Andres, Medical Research Council (UK), University College London, Francis Crick Institute, Cancer Research UK, Wellcome Trust, Chaves-Arquero, Belén, Abis, Giancarlo, Kelly, Geoff, Christodoulou, Evangelos, Taylor, Ian A., and Ramos, Andrés
Subjects: Model organisms, Chemical Biology & High Throughput, Transient protein-RNA interactions, Fragile X, RNA recognition, Method, Infectious Disease, RNA-binding proteins, FMRP, NMR, Imaging, Structural Biology & Biophysics
Abstract: 13 p.-4 fig.-1 graph. abst., Understanding how the RNA-binding domains of a protein regulator are used to recognize its RNA targets is a key problem in RNA biology, but RNA-binding domains with very low affinity do not perform well in the methods currently available to characterize protein-RNA interactions. Here, we propose to use conservative mutations that enhance the affinity of RNA-binding domains to overcome this limitation. As a proof of principle, we have designed and validated an affinity-enhanced K-homology (KH) domain mutant of the fragile X syndrome protein FMRP, a key regulator of neuronal development, and used this mutant to determine the domain’s sequence preference and to explain FMRP recognition of specific RNA motifs in the cell. Our results validate our concept and our nuclear magnetic resonance (NMR)-based workflow. While effective mutant design requires an understanding of the underlying principles of RNA recognition by the relevant domain type, we expect the method will be used effectively in many RNA-binding domains., This work was supported by the UK Medical Research Council (MC_PC_13051, U117574558, and MR/S000305/1) and the UK BBRSC research grant S014438/1. It was also supported by University College London and by the Francis Crick Institute, which receives its core funding from Cancer Research UK (CC2029), the UK Medical Research Council (CC2029), and the Wellcome Trust (CC2029). NMR spectra were recorded at the MRC Biomedical NMR Facility Francis Crick Institute UK, which is funded by Cancer Research UK (CC1078), the UK Medical Research Council (CC1078), and the Wellcome Trust (CC1078) and at UCL NMR facilities.
Published: 2023

16. Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Author: Global Health Research Unit on Global Surgery, National Institute for Health and Care Research, Adisa, Adewale, Bahrami-Hessari, Michael, Bhangu, Aneel, George, Christina, Ghosh, Dhruv, Glasbey, James, Haque, Parvez, Ingabire, J C Allen, Kamarajah, Sivesh Kathir, Kudrna, Laura, Ledda, Virginia, Li, Elizabeth, Lillywhite, Robert, Mittal, Rohin, Nepogodiev, Dmitri, Ntirenganya, Faustin, Picciochi, Maria, Simões, Joana Filipa Ferreira, Booth, L, Elliot, R, Kennerton, A S, Pettigrove, K L, Pinney, L, Richard, H, Tottman, R, Wheatstone, P, Wolfenden, John W D, Smith, A, Sayed, A Emad, Goswami, Aakansha Giri, Malik, Aamer, Mclean, Aaron Lawson, Hassan, Abbas, Nazimi, Abd Jabar, Aladna, Abdallah, Abdelgawad, Abdalrahman, Saed, Abdel, Abdelmageed, Abdelfatah, Ghannam, Abdelilah, Mahmoud, Abdelrahman, Alvi, Abdul, Ismail, Abdulaziz, Adesunkanmi, Abdulhafiz, Ebrahim, Abdulla, Al-Mallah, Abdullah, Alqallaf, Abdullah, Durrani, Abdullah, Gabr, Abdullah, Kirfi, Abdullahi Musa, Altaf, Abdulmalik, Almutairi, Abdulmjeed, Sabbagh, Abdulrahman J, Ajiya, Abdulrazak, Haddud, Abdurrahman, Alnsour, Abed Alfattah Mahmoud, Singh, Abhinav, Mittal, Abhishek, Semple, Abigail, Adeniran, Abiodun, Negussie, Abraham, Oladimeji, Abraham, Muhammad, Abubakar Bala, Yassin, Abubaker, Gungor, Abuzer, Tarsitano, Achille, Soibiharry, Adaiah, Dyas, Adam, Frankel, Adam, Peckham-Cooper, Adam, Truss, Adam, Issaka, Adamu, Ads, Adel Mohamed, Aderogba, Adeleke Akeem, Adeyeye, Ademola, Ademuyiwa, Adesoji, Sleem, Adham, Papa, Adrian, Cordova, Adriana, Appiah-Kubi, Adu, Meead, Adullah, Nacion, Aeris Jane D, Michael, Afieharo, Forneris, Agustin Albani, Duro, Agustin, Gonzalez, Agustin Rodriguez, Altouny, Ahmad, Ghazal, Ahmad, Khalifa, Ahmad, Ozair, Ahmad, Quzli, Ahmad, Haddad, Ahmad, Othman, Ahmad Faidzal, Yahaya, Ahmad Shuib, Elsherbiny, Ahmed, Nazer, Ahmed, Tarek, Ahmed, Abu-Zaid, Ahmed, Al-Nusairi, Ahmed, Azab, Ahmed, Elagili, Ahmed, Elkazaz, Ahmed, Kedwany, Ahmed, Nuhu, Ahmed Mohammed, Sakr, Ahmed, Shehta, Ahmed, Shirazi, Ahmed, Mohamed, Ahmed Mohamed Ibrahim, Sherif, Ahmed Elshawadfy, Awad, Ahmed K, Abbas, Ahmed M, Abdelrahman, Ahmed Saber, Ammar, Ahmed Siddique, Azzam, Ahmed Y, Ciftci, Ahmet Burak, Dural, Ahmet Cem, Sanli, Ahmet Necati, Rahy-Martín, Aida Cristina, Tantri, Aida Rosita, Khan, Aimal, Al-Touny, Aiman, Tariq, Aiman, Gmati, Aimen, Costas-Chavarri, Ainhoa, Auerkari, Aino, Landaluce-Olavarria, Aitor, Puri, Ajay, Radhakrishnan, Ajay, Ubom, Akaninyene Eseme, Pradhan, Akhilesh, Turna, Akif, Adepiti, Akinfolarin, Kuriyama, Akira, Kassam, Al-Faraaz, Hassouneh, Ala, El-Hussuna, Alaa, Habeebullah, Alaa, Ads, Alaa Mohamed, Mousli, Alaa, Biloslavo, Alan, Hoang, Alan, Kirk, Alan, Santini, Alasdair, Melero, Alba Vazquez, Calvache, Albaro José Nieto, Baduell, Albert, Chan, Albert, Abrate, Alberto, Balduzzi, Alberto, Sánchez, Alberto Cabañero, Navarrete-Peón, Alberto, Porcu, Alberto, Brolese, Alberto, Barranquero, Alberto G, Saibene, Alberto Maria, Adam, Albushra Altayeb, 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Subjects: general surgery, acute care surgery, Settore MED/18 - CHIRURGIA GENERALE, climate change, trauma, Settore MED/40 - Ginecologia E Ostetricia, environmental impact, surgery, Settore MED/31 - Otorinolaringoiatria, None, Medicine and Health Sciences, Surgery, operating theatre, environment
Abstract: Background: Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods: This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low-middle-income countries. Results: In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of 'single-use' consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low-middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion: This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high- and low-middle-income countries.
Published: 2023

17. Functional correlation of genome-wide DNA methylation profiles in genetic neurodevelopmental disorders

Author: Michael A. Levy, Raissa Relator, Haley McConkey, Erinija Pranckeviciene, Jennifer Kerkhof, Mouna Barat‐Houari, Sara Bargiacchi, Elisa Biamino, María Palomares Bralo, Gerarda Cappuccio, Andrea Ciolfi, Angus Clarke, Barbara R. DuPont, Mariet W. Elting, Laurence Faivre, Timothy Fee, Marco Ferilli, Robin S. Fletcher, Florian Cherick, Aidin Foroutan, Michael J. Friez, Cristina Gervasini, Sadegheh Haghshenas, Benjamin A. Hilton, Zandra Jenkins, Simranpreet Kaur, Suzanne Lewis, Raymond J. Louie, Silvia Maitz, Donatella Milani, Angela T. Morgan, Renske Oegema, Elsebet Østergaard, Nathalie R. Pallares, Maria Piccione, Astrid S. Plomp, Cathryn Poulton, Jack Reilly, Rocio Rius, Stephen Robertson, Kathleen Rooney, Justine Rousseau, Gijs W. E. Santen, Fernando Santos‐Simarro, Josephine Schijns, Gabriella M. Squeo, Miya St John, Christel Thauvin‐Robinet, Giovanna Traficante, Pleuntje J. van der Sluijs, Samantha A. Vergano, Niels Vos, Kellie K. Walden, Dimitar Azmanov, Tugce B. Balci, Siddharth Banka, Jozef Gecz, Peter Henneman, Jennifer A. Lee, Marcel M. A. M. Mannens, Tony Roscioli, Victoria Siu, David J. Amor, Gareth Baynam, Eric G. Bend, Kym Boycott, Nicola Brunetti‐Pierri, Philippe M. Campeau, Dominique Campion, John Christodoulou, David Dyment, Natacha Esber, Jill A. Fahrner, Mark D. Fleming, David Genevieve, Delphine Heron, Thomas Husson, Kristin D. Kernohan, Alisdair McNeill, Leonie A. Menke, Giuseppe Merla, Paolo Prontera, Cheryl Rockman‐Greenberg, Charles Schwartz, Steven A. Skinner, Roger E. Stevenson, Marie Vincent, Antonio Vitobello, Marco Tartaglia, Marielle Alders, Matthew L. Tedder, Bekim Sadikovic, Human genetics, Amsterdam Reproduction & Development (AR&D), Pediatrics, Levy M.A., Relator R., McConkey H., Pranckeviciene E., Kerkhof J., Barat-Houari M., Bargiacchi S., Biamino E., Palomares Bralo M., Cappuccio G., Ciolfi A., Clarke A., DuPont B.R., Elting M.W., Faivre L., Fee T., Ferilli M., Fletcher R.S., Cherick F., Foroutan A., Friez M.J., Gervasini C., Haghshenas S., Hilton B.A., Jenkins Z., Kaur S., Lewis S., Louie R.J., Maitz S., Milani D., Morgan A.T., Oegema R., Ostergaard E., Pallares N.R., Piccione M., Plomp A.S., Poulton C., Reilly J., Rius R., Robertson S., Rooney K., Rousseau J., Santen G.W.E., Santos-Simarro F., Schijns J., Squeo G.M., John M.S., Thauvin-Robinet C., Traficante G., van der Sluijs P.J., Vergano S.A., Vos N., Walden K.K., Azmanov D., Balci T.B., Banka S., Gecz J., Henneman P., Lee J.A., Mannens M.M.A.M., Roscioli T., Siu V., Amor D.J., Baynam G., Bend E.G., Boycott K., Brunetti-Pierri N., Campeau P.M., Campion D., Christodoulou J., Dyment D., Esber N., Fahrner J.A., Fleming M.D., Genevieve D., Heron D., Husson T., Kernohan K.D., McNeill A., Menke L.A., Merla G., Prontera P., Rockman-Greenberg C., Schwartz C., Skinner S.A., Stevenson R.E., Vincent M., Vitobello A., Tartaglia M., Alders M., Tedder M.L., Sadikovic B., Levy, Michael A, Relator, Raissa, Mcconkey, Haley, Pranckeviciene, Erinija, Kerkhof, Jennifer, Barat-Houari, Mouna, Bargiacchi, Sara, Biamino, Elisa, Bralo, María Palomare, Cappuccio, Gerarda, Ciolfi, Andrea, Clarke, Angu, Dupont, Barbara R, Elting, Mariet W, Faivre, Laurence, Fee, Timothy, Ferilli, Marco, Fletcher, Robin S, Cherick, Florian, Foroutan, Aidin, Friez, Michael J, Gervasini, Cristina, Haghshenas, Sadegheh, Hilton, Benjamin A, Jenkins, Zandra, Kaur, Simranpreet, Lewis, Suzanne, Louie, Raymond J, Maitz, Silvia, Milani, Donatella, Morgan, Angela T, Oegema, Renske, Østergaard, Elsebet, Pallares, Nathalie Ruiz, Piccione, Maria, Plomp, Astrid S, Poulton, Cathryn, Reilly, Jack, Rius, Rocio, Robertson, Stephen, Rooney, Kathleen, Rousseau, Justine, Santen, Gijs W E, Santos-Simarro, Fernando, Schijns, Josephine, Squeo, Gabriella Maria, John, Miya St, Thauvin-Robinet, Christel, Traficante, Giovanna, van der Sluijs, Pleuntje J, Vergano, Samantha A, Vos, Niel, Walden, Kellie K, Azmanov, Dimitar, Balci, Tugce B, Banka, Siddharth, Gecz, Jozef, Henneman, Peter, Lee, Jennifer A, Mannens, Marcel M A M, Roscioli, Tony, Siu, Victoria, Amor, David J, Baynam, Gareth, Bend, Eric G, Boycott, Kym, Brunetti-Pierri, Nicola, Campeau, Philippe M, Campion, Dominique, Christodoulou, John, Dyment, David, Esber, Natacha, Fahrner, Jill A, Fleming, Mark D, Genevieve, David, Heron, Delphine, Husson, Thoma, Kernohan, Kristin D, Mcneill, Alisdair, Menke, Leonie A, Merla, Giuseppe, Prontera, Paolo, Rockman-Greenberg, Cheryl, Schwartz, Charle, Skinner, Steven A, Stevenson, Roger E, Vincent, Marie, Vitobello, Antonio, Tartaglia, Marco, Alders, Marielle, Tedder, Matthew L, Sadikovic, Bekim, Human Genetics, General Paediatrics, Graduate School, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, ARD - Amsterdam Reproduction and Development, ANS - Cellular & Molecular Mechanisms, ANS - Complex Trait Genetics, and ACS - Pulmonary hypertension & thrombosis
Subjects: DNA methylation, clinical diagnostics, Syndrome, DNA methylation, clinical diagnostics, episignatures, neurodevelopmental syndromes, neurodevelopmental syndromes, Epigenesis, Genetic, Neurodevelopmental Disorders, Genetics, Humans, CpG Islands, DNA, Intergenic, episignatures, Episignature, Genetics (clinical)
Abstract: An expanding range of genetic syndromes are characterized by genome-wide disruptions in DNA methylation profiles referred to as episignatures. Episignatures are distinct, highly sensitive and specific biomarkers that have recently been applied in clinical diagnosis of genetic syndromes. Episignatures are contained within the broader disorder-specific genome-wide DNA methylation changes which can share significant overlap amongst different conditions. In this study we performed functional genomic assessment and comparison of disorder-specific and overlapping genome-wide DNA methylation changes related to 65 genetic syndromes with previously described episignatures. We demonstrate evidence of disorder-specific and recurring genome-wide differentially methylated probes (DMPs) and regions (DMRs). The overall distribution of DMPs and DMRs across the majority of the neurodevelopmental genetic syndromes analyzed showed substantial enrichment in gene promoters and CpG islands, and under-representation of the more variable intergenic regions. Analysis showed significant enrichment of the DMPs and DMRs in gene pathways and processes related to neurodevelopment, including neurogenesis, synaptic signaling and synaptic transmission. This study expands beyond the diagnostic utility of DNA methylation episignatures by demonstrating correlation between the function of the mutated genes and the consequent genomic DNA methylation profiles as a key functional element in the molecular etiology of genetic neurodevelopmental disorders. This article is protected by copyright. All rights reserved.
Published: 2022

18. Sodium Glucose Cotransporter-2 Inhibitor Empagliflozin Reduces Infarct Size Independently of Sodium Glucose Cotransporter-2

Author: Sha Chen, Qian Wang, Andriana Christodoulou, Nikolaos Mylonas, Diane Bakker, Rianne Nederlof, Markus W. Hollmann, Nina C. Weber, Ruben Coronel, Vincent Wakker, Vincent M. Christoffels, Ioanna Andreadou, Coert J. Zuurbier, Anesthesiology, Graduate School, ACS - Microcirculation, APH - Quality of Care, ACS - Heart failure & arrhythmias, ACS - Atherosclerosis & ischemic syndromes, ACS - Diabetes & metabolism, AII - Infectious diseases, AII - Inflammatory diseases, Amsterdam Cardiovascular Sciences, Experimental Cardiology, Medical Biology, and Amsterdam Reproduction & Development (AR&D)
Subjects: Physiology (medical), Cardiology and Cardiovascular Medicine
Published: 2023

19. Designing and Implementing Effective Policies to Promote Dual career through Sports Schools

Author: Diana Christodoulou
Abstract: Evaluations of educational policies continue to be a critical issue since they must provide the authorities with a clear understanding of the procedures and practices, they need to develop to become more successful. The purpose of this article was the assessment of the evaluation process of the sports school in Cyprus taking into account the objective model. The study concluded with possible implications for other countries related to policy design and emphasized that evaluating the effectiveness of a policy is crucial and undoubtedly, the objective model might lead to an effective implementation.
Published: 2023

20. MOGS-CDG: Quantitative analysis of the diagnostic Glc(3) Man tetrasaccharide and clinical spectrum of six new cases

Author: Merel A. Post, Isis de Wit, Fokje S. M. Zijlstra, Udo F. H. Engelke, Arno van Rooij, John Christodoulou, Tiong Yang Tan, Anna Le Fevre, Danqun Jin, Joy Yaplito‐Lee, Beom Hee Lee, Karen J. Low, Andrew A. Mallick, Katrin Õunap, James Pitt, William Reardon, Mari‐Anne Vals, Saskia B. Wortmann, Hans J. C. T. Wessels, Melissa Bärenfänger, Clara D. M. van Karnebeek, and Dirk J. Lefeber
Subjects: All institutes and research themes of the Radboud University Medical Center, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], Genetics, Metabolic Disorders Radboud Institute for Molecular Life Sciences [Radboudumc 6], Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3], Genetics (clinical)
Abstract: Contains fulltext : 290862.pdf (Publisher’s version ) (Open Access) Congenital disorders of glycosylation (CDG) are a clinically and biochemically heterogeneous subgroup of inherited metabolic disorders. Most CDG with abnormal N-glycosylation can be detected by transferrin screening, however, MOGS-CDG escapes this routine screening. Combined with the clinical heterogeneity of reported cases, diagnosing MOGS-CDG can be challenging. Here, we clinically characterize ten MOGS-CDG cases including six previously unreported individuals, showing a phenotype characterized by dysmorphic features, global developmental delay, muscular hypotonia, and seizures in all patients and in a minority vision problems and hypogammaglobulinemia. Glycomics confirmed accumulation of a Glc(3) Man(7) GlcNAc(2) glycan in plasma. For quantification of the diagnostic Glcα1-3Glcα1-3Glcα1-2Man tetrasaccharide in urine, we developed and validated a liquid chromatography-mass spectrometry method of 2-aminobenzoic acid (2AA) labeled urinary glycans. As an internal standard, isotopically labeled (13) C(6) -2AA Glc(3) Man was used, while labeling efficiency was controlled by use of (12) C(6) -2AA and (13) C(6) -2AA labeled laminaritetraose. Recovery, linearity, intra- and interassay coefficients of variability of these labeled compounds were determined. Furthermore, Glc(3) Man was specifically identified by retention time matching against authentic MOGS-CDG urine and compared with Pompe urine. Glc(3) Man was increased in all six analyzed cases, ranging from 34.1 to 618.0 μmol/mmol creatinine (reference
Published: 2023

21. PathIN: an integrated tool for the visualization of pathway interaction networks

Author: George Minadakis, Kyproula Christodoulou, George Tsouloupas, and George M. Spyrou
Subjects: Structural Biology, Genetics, Biophysics, Biochemistry, Computer Science Applications, Biotechnology
Abstract: PathIN is a web-service that provides an easy and flexible way for rapidly creating pathway-based networks at several functional biological levels: genes, compounds and reactions. The tool is supported by a database repository of reference pathway networks across a large set of species, developed through the freely available information included in the KEGG, Reactome and Wiki Pathways database repositories. PathIN provides networks by means of five diverse methodologies: (a) direct connections between pathways of interest, (b) direct connections as well as the first neighbours of the given pathways, (c) direct connections, the first neighbours and the connections in between them, and (d) two additional methodologies for creating complementary pathway-to-pathway networks that involve additional (missing) pathways that interfere in-between pathways of interest. PathIN is expected to be used as a simple yet informative reference tool for understanding networks of molecular mechanisms related to specific diseases.
Published: 2023

22. Source detection with multi-label classification

Author: Jayakrishnan Vijayamohanan, Arjun Gupta, Oameed Noakoasteen, Sotirios K. Goudos, and Christos G Christodoulou
Subjects: Signal Processing
Published: 2023

23. Exploring the gene pools of Cypriot oaks: no evidence of intersectional hybridization

Author: Charalambos Neophytou, Simon Jansen, Ralf Hand, Georgia Chrysostomou, Konstantinos Iosif, and Charalambos Christodoulou
Subjects: Genetics, Forestry
Abstract: In our study, we performed a population genetic analysis in all three native oak species of Cyprus and addressed whether two intermediate individuals arose from intersectional hybridization between Quercus coccifera ssp. calliprinos and Q. infectoria ssp. veneris. For this purpose, we successfully tested chloroplast and nuclear microsatellites initially developed for other oak species. We identified a set of markers that can be efficiently used for species identification and study of hybridization. Haplotypes based on the chloroplast microsatellites could resolve different maternal lineages and distinguish between the two sections represented in the study area. Using the nuclear microsatellites, we described patterns of genetic diversity across species with the endemic Q. alnifolia exhibiting reduced genetic diversity. Additionally, we employed a multivariate analysis, which could clearly differentiate among the three species. The two intermediate individuals clustered within the point cloud of Q. infectoria ssp. veneris and also possessed a chloroplast haplotype typical for this species. Therefore, we rejected the hypothesis of intersectional hybridization and interpreted their phenotypic appearance as the result of high phenotypic plasticity within Q. infectoria ssp. veneris.
Published: 2023

24. Semantic models and services for conservation and restoration of cultural heritage: A comprehensive survey

Author: Efthymia Moraitou, Yannis Christodoulou, and George Caridakis
Subjects: Computer Networks and Communications, Computer Science Applications, Information Systems
Abstract: Over the last decade, the Cultural Heritage (CH) domain has gradually adopted Semantic Web (SW) technologies for organizing information and for tackling interoperability issues. Several semantic models have been proposed which accommodate essential aspects of information management: retrieval, integration, reuse and sharing. In this context, the CH subdomain of Conservation and Restoration (CnR) exhibits an increasing interest in SW technologies, in an attempt to effectively handle the highly heterogeneous and often secluded CnR information. This paper investigates semantic models relevant to the CnR knowledge domain. The scope, development methodology and coverage of CnR aspects are described and discussed. Furthermore, the evaluation, deployment and current exploitation of each model are examined, with focus on the types and variety of services provided to support the CnR professional. Through this study, the following research questions are investigated: To what extent the various aspects of CnR are covered by existing CnR models? To what extent existing CnR models incorporate models of the broader CH domain and of relevant disciplines (e.g., Chemistry)? In what ways and to what extent services built upon the reviewed models facilitate CnR professionals in their various tasks? Finally, based on the findings, fields of interest that merit further investigation are suggested.
Published: 2022

25. Stapled diaphragm resection: A new approach to diaphragmatic cytoreductive surgery for advanced-stage ovarian cancer

Author: Daniela Huber, Ian Fournier, Michel Christodoulou, Stéphanie Seidler, Valérie Besse, Marie-Pierre Mathey, James Nef, and Yannick Hurni
Subjects: Ovarian Neoplasms, Reproductive Medicine, Diaphragm, Humans, Obstetrics and Gynecology, Female, Cytoreduction Surgical Procedures, Carcinoma, Ovarian Epithelial, Retrospective Studies
Abstract: To evaluate a novel technique for diaphragmatic full-thickness resection (DFTR) using a vascular stapler to perform cytoreductive surgeries in patients with advanced ovarian cancer.Single-center retrospective analysis of consecutive patients with advanced-stage ovarian cancer undergoing stapled diaphragmatic full-thickness resections (S-DFTRs) as part of cytoreductive surgeries between January 2018 and June 2022, according to the IDEAL recommendations.Fifteen patients underwent cytoreductive surgeries with S-DFTRs. The median operative time was 300 (114-547) minutes. Cytoreduction was considered complete in all cases. All S-DFTRs were performed on the right diaphragm. Concomitant left diaphragmatic peritoneal stripping was performed in 5 cases (33.3%) and was associated with a conventional DFTR in 1 case (6.7%). Prophylactic intraoperative tube thoracostomy was never required. Four patients (26.7%) were admitted to the intensive care unit. Pleural effusion was observed in 9 patients (60.0%), and 4 (26.7%) required a postoperative pigtail catheter thoracostomy. Three patients (20.0%) required catheter placement on the right hemithorax (ipsilaterally to the S-DFTR) and 2 patients (13.3%) required catheters on the left hemithorax (contralaterally to the S-DFTR). Pneumothorax requiring tube thoracostomy was observed in 1 case (6.7%) on the left hemithorax (contralaterally to the S-DFTR). Pulmonary embolism and pneumonia were both observed once (6.7%). The median hospitalization length was 14 (5-36) days. During the follow-up, 6 patients (40.0%) had a recurrence, but none involved the pleura or the diaphragm. According to the IDEAL classification, this study could be ranked as stage 2a (development).This technique appears to be a fast and safe method for performing diaphragmatic cytoreductive surgeries and could reduce postoperative complications.
Published: 2022

26. LARS2 variants can present as premature ovarian insufficiency in the absence of overt hearing loss

Author: Anne Sophie Neyroud, Joëlle Rudinger-Thirion, Magali Frugier, Lisa G. Riley, Maud Bidet, Linda Akloul, Andrea Simpson, David Gilot, John Christodoulou, Célia Ravel, Andrew H. Sinclair, Marc-Antoine Belaud-Rotureau, Elena J. Tucker, Sylvie Jaillard, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CHU Pontchaillou [Rennes], Architecture et Réactivité de l'ARN (ARN), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), The University of Sydney, Clinique mutualiste La Sagesse, La Trobe University [Melbourne], Charles Darwin University [Australia], Oncogenesis, Stress, Signaling (OSS), Université de Rennes (UR)-CRLCC Eugène Marquis (CRLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Melbourne, Murdoch Children's Research Institute (MCRI), and This work was supported by a CHU Rennes grant (Appel à Projets Innovations 2019 to SJ), an Australian NHMRC program grant (1074258 to AHS), NHMRC fellowships (1054432 to EJT, 1062854 to AHS) and an Australian Mito Foundation grant (EJT). Part of this work was performed under the Rare Diseases Functional Genomics program, supported by the Luminesce Alliance—Innovation for Children’s Health, a not for profit cooperative joint venture between the Sydney Children’s Hospitals Network, the Children’s Medical Research Institute, and the Children’s Cancer Institute. It has been established with the support of the NSW Government to coordinate and integrate pediatric research. Luminesce Alliance is also affiliated with the University of Sydney and the University of New South Wales Sydney (LGR). The research conducted at the Murdoch Children’s Research Institute was supported by the Victorian government’s operational infrastructure support program. The Chair in Genomic Medicine awarded to JC is generously supported by The Royal Children’s Hospital Foundation.
Subjects: [SDV.GEN]Life Sciences [q-bio]/Genetics, Genetics, Genetics (clinical)
Abstract: International audience; Premature ovarian insufficiency (POI) affects 1 in 100 women and is a leading cause of female infertility. There are over 80 genes in which variants can cause POI, with these explaining only a minority of cases. Whole exome sequencing (WES) can be a useful tool for POI patient management, allowing clinical care to be personalized to underlying cause. We performed WES to investigate two French sisters, whose only clinical complaint was POI. Surprisingly, they shared one known and one novel likely pathogenic variant in the Perrault syndrome gene, LARS2. Using amino-acylation studies, we established that the novel missense variant significantly impairs LARS2 function. Perrault syndrome is characterized by sensorineural hearing loss in addition to POI. This molecular diagnosis alerted the sisters to the significance of their difficulty in following conversation. Subsequent audiology assessment revealed a mild bilateral hearing loss. We describe the first cases presenting with perceived isolated POI and causative variants in a Perrault syndrome gene. Our study expands the phenotypic spectrum associated with LARS2 variants and highlights the clinical benefit of having a genetic diagnosis, with prediction of potential co-morbidity and prompt and appropriate medical care, in this case by an audiologist for early detection of hearing loss.
Published: 2022

27. A low-cost pavement-rating system, based on machine learning, utilising smartphone sensors

Author: Charalambos Kyriakou and Symeon E Christodoulou
Subjects: General Medicine
Abstract: One of the key innovations of this study is the development of a cost-efficient approach for pavement monitoring. This work aims to develop a system that evaluates roadway pavement surface conditions with improved frequency by utilising unsupervised machine learning algorithms and smartphone sensors. The evaluation of roadways utilising complex contemporary data sets is currently conducted periodically because of the high cost of collection methods. For this purpose, the study presents a data-driven framework on the use of a vehicle, a smartphone, an on-board diagnostic device and machine learning for the rating of pavement surfaces, while statistical features are considered in both time- and frequency-domain forms. The selection of features is performed utilising unsupervised classification algorithms. Moreover, the proposed system architecture has been field-tested for the detection of pavement anomalies and the classification of five rating categories. Furthermore, the proposed system may provide daily information on roadway pavement surface conditions, which can be used by agencies for automating the planning of pavement maintenance operations and for improving driving safety.
Published: 2022

28. Tomorrow’s History in the Museum. Collecting Testimonies of Current Events for the House of European History

Author: Perikles Christodoulou, Simina Bădică, Raluca Bem Neamu, and Jitka Mlsová Chmelíková
Abstract: Contemporary collecting relies on the expertise of museum professionals. They identify, acquire, and safeguard current objects and testimonies that can illustrate and challenge history writing in the future. This paper presents the contemporary collecting endeavours of the House of European History—a museum that opened its doors in Brussels in 2017, as an academically independent project of the European Parliament. It contributes to the current discussion on best practices in collecting materials relevant to present-day society and documenting history in the making. In particular, it stresses the importance for a history museum to document significant aspects of the present and focuses on two recent collecting actions of the House, which were undertaken to document the COVID-19 pandemic and war in Ukraine.
Published: 2022

29. Splenic Artery Infarct Requiring Surgery: A Rare Complication of COVID-19 Infection

Author: Ioannis Dimitriou, Nikolaos Christodoulou, Kleanthis Chatzimargaritis, Aristidis Kaikis, Eirini Kasti, and Georgios Triantos
Subjects: Pharmacology (medical)
Abstract: Introduction. Coronavirus disease (COVID-19) from SARS-CoV-2 infection is linked to a hypercoagulable state, leading to arterial and venous thrombotic events, of which pulmonary embolism is the most frequent. However, arterial thromboembolisms may also occur as visceral infracts in unusual sites, such as the renal, splenic, and intestinal arteries. Case Report. A 46-year-old unvaccinated male with a COVID-19 infection was admitted to the COVID-19 isolation ward with symptoms of respiratory infection. He complained of epigastric pain and fever for several days; radiological imaging of the abdomen revealed complete splenic arterial occlusion due to a large infarct. He was treated with low molecular weight heparin (enoxaparin) in therapeutic doses, resulting in minimal improvement. However, the pain worsened, and eventually, a laparotomy and splenectomy were performed. He was hospitalized for another 36 days before he was discharged in good condition. A second surgery was performed to remove a noninfected encapsulated hematoma from the subdiaphragmatic space. The patient remained healthy afterward, with no relapses. Discussion. Although rare, the number of cases of visceral infarcts in COVID-19 patients has increased. Splenic artery infarct is an exceptional case of acute abdominal pain that can be treated successfully with anticoagulant medication. Splenectomy may be required to manage refractory pain after failure of conservative management.
Published: 2022

30. Towards the Internet of MetaMaterial Things: Software Enablers for User‐Customizable Electromagnetic Wave Propagation

Author: Christos Liaskos, Georgios G. Pyrialakos, Alexandros Pitilakis, Ageliki Tsioliaridou, Michail Christodoulou, Nikolaos Kantartzis, Sotiris Ioannidis, Andreas Pitsillides, and Ian F. Akyildiz
Published: 2022

31. Risk and resilience correlates of reading among adolescents with language-based learning disabilities during COVID-19

Author: Rebecca A. Marks, Rachel T. Norton, Laura Mesite, Annie B. Fox, and Joanna A. Christodoulou
Subjects: Speech and Hearing, Linguistics and Language, Neuropsychology and Physiological Psychology, Education
Abstract: Students with language-based learning disabilities (LBLD) can face elevated socio-emotional well-being challenges in addition to literacy challenges. We examined the prevalence of risk and resilience factors among adolescents with LBLD (N = 93), ages 16–18, and the association with reading performance during the COVID-19 pandemic. Data were collected at the start and end of the first fully remote academic year of COVID-19 (2020–2021). Participants completed standardized word and text reading measures, as well as self-report surveys of executive functions (EF), and socio-emotional skills associated with resilience (grit, growth mindset, self-management, self-efficacy, and social awareness) or risk (anxiety, depression, COVID-19 related PTSD, and perceived COVID-19 impact). Survey data at the start of the school year (Time 1) captured three underlying factors associated with socioemotional risk, socioemotional resilience, and regulation (i.e., EF). Path analyses revealed that students’ Time 2 oral reading scores were significantly and uniquely predicted by socioemotional resilience, even when controlling for word-level reading at Time 1. Socioemotional risk, EF, and perceived COVID-19 impact were not directly related to Time 2 oral reading scores; however, students’ resilience mediated the associations between risk and reading outcomes. These results demonstrate that adolescents’ mental health concerns, self-regulatory ability, and socioemotional resilience were all associated with their experiences of the COVID-19-related stress. However, despite the high-risk context of the pandemic, and socio-emotional challenges faced by students with LBLD, our findings indicate that resilience directly predicts end-of-year reading outcomes and mediates the impact of socioemotional risk on achievement.
Published: 2022

32. Identification of Risk Factors of Recurrence Among Patients With Vulvar Paget Disease Treated With Conservative Surgery

Author: Vasilios Pergialiotis, Theano Christodoulou, Loukas Ferousis, Nikolaos Thomakos, Alexandros Rodolakis, and Dimitrios Haidopoulos
Subjects: Obstetrics and Gynecology, General Medicine
Abstract: Vulvar Paget disease (VPD) is a benign disease with high recurrence rates. Standard treatment involves conservative surgery with wide local excision of the lesion. The purpose of the present study is to identify factors that increase the risk of relapse.We conducted a retrospective study and included patients treated with conservative surgery for noninvasive VPD. Cox regression analysis was carried out to assess the independent effect of age, presence of positive margins, tumor size greater than 4 cm, bilateral lesions, and compositive morbidity and pathology on recurrence free survival. Post hoc power analysis was performed with the G-power tool using an α error of 0.05.Overall, 39 patients were included with a median age of 70 years (46-85 years). Of those, 19 patients relapsed within a median duration of 30.5 months (5-132 months). Twelve patients (63%) experienced at least a second relapse. The presence of composite comorbidity significantly affected the interval to recurrence (30.09 vs 71.80 months, p = .032). Univariate Cox regression analysis revealed that the presence of composite pathology features was indicative of a higher risk of recurrence (hazard ratio = -3.71, p = .024). The sample size did not allow for adequate power for this latter finding. Microscopically involved tumor margins and tumor size greater than 4 cm did not predict patients at risk of experiencing relapsing disease.Patients with noninvasive VPD experience high relapse rates. The presence of concurrent benign vulvar pathology may increase these rates, although larger sample sizes are needed to ascertain our findings.
Published: 2022

33. Thermal transitions, interfacial interactions, and molecular mobility in nanocomposites based on poly(l,d-lactic acid) and fumed silica nanoparticles

Author: Panagiotis A. Klonos, Evi Christodoulou, Theodora C. Katsika, Chrysanthi Papoulia, Konstantinos Chrissafis, Apostolos Kyritsis, and Dimitrios N. Bikiaris
Subjects: Physical and Theoretical Chemistry, Condensed Matter Physics
Published: 2022

34. <scp>MR</scp> Multitasking‐based multi‐dimensional assessment of cardiovascular system ( <scp>MT‐MACS</scp> ) with extended spatial coverage and <scp>water‐fat</scp> separation

Author: Zhehao Hu, Jiayu Xiao, Xianglun Mao, Yibin Xie, Alan C. Kwan, Shlee S. Song, Michael W. Fong, Alison G. Wilcox, Debiao Li, Anthony G. Christodoulou, and Zhaoyang Fan
Subjects: Radiology, Nuclear Medicine and imaging
Abstract: To extend the MR MultiTasking-based Multidimensional Assessment of Cardiovascular System (MT-MACS) technique with larger spatial coverage and water-fat separation for comprehensive aortocardiac assessment.MT-MACS adopts a low-rank tensor image model for 7D imaging, with three spatial dimensions for volumetric imaging, one cardiac motion dimension for cine imaging, one respiratory motion dimension for free-breathing imaging, one T2-prepared inversion recovery time dimension for multi-contrast assessment, and one T2*-decay time dimension for water-fat separation. Nine healthy subjects were recruited for the 3T study. Overall image quality was scored on bright-blood (BB), dark-blood (DB), and gray-blood (GB) contrasts using a 4-point scale (0-poor to 3-excellent) by two independent readers, and their interreader agreement was evaluated. Myocardial wall thickness and left ventricular ejection fraction (LVEF) were quantified on DB and BB contrasts, respectively. The agreement in these metrics between MT-MACS and conventional breath-held, electrocardiography-triggered 2D sequences were evaluated.MT-MACS provides both water-only and fat-only images with excellent image quality (average score = 3.725/3.780/3.835/3.890 for BB/DB/GB/fat-only images) and moderate to high interreader agreement (weighted Cohen's kappa value = 0.727/0.668/1.000/1.000 for BB/DB/GB/fat-only images). There were good to excellent agreements in myocardial wall thickness measurements (intraclass correlation coefficients [ICC] = 0.781/0.929/0.680/0.878 for left atria/left ventricle/right atria/right ventricle) and LVEF quantification (ICC = 0.716) between MT-MACS and 2D references. All measurements were within the literature range of healthy subjects.The refined MT-MACS technique provides multi-contrast, phase-resolved, and water-fat imaging of the aortocardiac systems and allows evaluation of anatomy and function. Clinical validation is warranted.
Published: 2022

35. Integrating the Global Agenda of Social Work and Social Development in the Republic of Cyprus

Author: Panagiotis Pentaris, Panayiota Christodoulou, Koulla Erotocritou, Stavros Parlalis, Demetris Hadjiharalambous, and Sue Hanna
Subjects: Sociology and Political Science, Social Sciences (miscellaneous)
Published: 2022

36. Ambient carbonaceous aerosol levels in Cyprus and the role of pollution transport from the Middle East

Author: Aliki Christodoulou and Christodoulou, Aliki
Abstract: These files contain NR-PM1, BC timeseries andOA source apportionmentresultsobtained from an ACSM deployed in the urban background station of Nicosiain the periodDecember 2018-October 2019.
Published: 2023
Full Text: View/download PDF

37. Factors Affecting Cement Suspensions Strength for Soil Formations Improvement – A Review

Author: Christodoulou Dimitrios
Subjects: History, Algebra and Number Theory, Literature and Literary Theory, Materials Science (miscellaneous), General Chemical Engineering, Organic Chemistry, Geology, General Chemistry, General Medicine, Biochemistry, Computational Theory and Mathematics, Geochemistry and Petrology, Economic Geology, Pharmacology (medical), Geometry and Topology, Physical and Theoretical Chemistry, General Agricultural and Biological Sciences
Abstract: Grouting is a common technical method with many applications, e.g., it is used for soil stabilization and strengthening, for reduction for water ingress to underground facilities or of the water loss through a dam foundation, etc. Grouts comprise several constituents, which are combined in many ways depending on the in-situ conditions and the outcome desired. The use of very fine cement grouts for injections into fine-to-medium sands has been proposed to circumvent problems associated with the permanence and toxicity of chemical grouts and the inability of ordinary cement grouts to permeate soil formations finer than coarse sand. The strength of a suspension is the property of probably most interest in cases where the main objective of a soak injection program is to improve the mechanical properties of the soil. The object of this paper is to investigate the effect of cement physical properties, water-to-cement ratio, additives and chemical improvers on the strength of cement suspensions.
Published: 2023

38. Novel de novo DNMT1 gene mutation associated with hereditary sensory and autonomic neuropathy 1E (HSAN1E)

Author: Dimitrios Parissis, Kyproula Christodoulou, and Kleopas A. Kleopa
Subjects: Psychiatry and Mental health, Neurology (clinical), Dermatology, General Medicine
Published: 2023

39. A brief review of Valsalva retinopathy

Author: Petros Asteris, Dimitrios Kalogeropoulos, George Moussa, Ioannis Iraklis Vitos, Aikaterini Christodoulou, and Chris Kalogeropoulos
Abstract: Background: Valsalva retinopathy is a specific type of retinopathy that appears following an acute rise in intrathoracic or intra-abdominal pressure. This review focuses on current literature and future directions for the diagnosis and management of Valsalva retinopathy. Methods: In this brief review, the literature was searched to provide an extensive general and updated description of Valsalva retinopathy, focusing on its management and prognosis. Selected articles are summarized to present the etiology, general pathology, pathophysiology, symptoms, signs, diagnosis, differential diagnosis, treatment, and prognosis of Valsalva retinopathy. Results: The main symptom of Valsalva retinopathy is loss of visual acuity following exertion. This is caused by the rupture of small superficial vessels in the macula and perimacular areas, leading to extravasation of blood. No link between age, sex, or race has been found, although fundus vessel abnormalities pose some predisposition to the disease. During fundoscopy, Valsalva retinopathy appears most frequently as well-defined preretinal hemorrhages confined to the sub-internal limiting membrane (ILM) or subhyaloid space. If ILM rupture occurs, hyaloid hemorrhage can appear. Diagnosis is based on the patient’s medical history and a standard examination. Usually, only observation of the patient is required, with the hemorrhage resolving within weeks to months. In cases of large premacular hemorrhage or large sub-ILM/subhyaloid hemorrhage, vitrectomy or Nd:YAG krypton laser membranotomy can be performed. Conclusions: Of all the aspects of Valsalva retinopathy that might trouble the physician, the most challenging features are differential diagnosis and the choice of optimal treatment. Therapeutic strategies for Valsalva retinopathy can be either conservative or based on Nd:YAG laser membranotomy and/or vitrectomy. All methods seem to have good outcomes. However, physicians should not be afraid of advancing beyond conservative therapy, especially in the event of persistent premacular hemorrhage, which could lead to permanent retinal damage if valuable time is lost, even after vitrectomy.
Published: 2022

40. A novel network pharmacology approach for leukaemia differentiation therapy using Mogrify®

Author: Lin Ming Lee, Eleni G. Christodoulou, Pavithra Shyamsunder, Bei Jun Chen, Kian Leong Lee, Tsz Kan Fung, Chi Wai Eric So, Gee Chuan Wong, Enrico Petretto, Owen J. L. Rackham, and S. Tiong Ong
Subjects: Leukemia, Myeloid, Acute, Cancer Research, Leukemia, Promyelocytic, Acute, Oncogene Proteins, Fusion, Genetics, Humans, Tretinoin, Cell Differentiation, Network Pharmacology, Molecular Biology, Transcription Factors
Abstract: Acute myeloid leukaemia (AML) is a rapidly fatal blood cancer that is characterised by the accumulation of immature myeloid cells in the blood and bone marrow as a result of blocked differentiation. Methods which identify master transcriptional regulators of AML subtype-specific leukaemia cell states and their combinations could be critical for discovering novel differentiation-inducing therapies. In this proof-of-concept study, we demonstrate a novel utility of the Mogrify ® algorithm in identifying combinations of transcription factors (TFs) and drugs, which recapitulate granulocytic differentiation of the NB4 acute promyelocytic leukaemia (APL) cell line, using two different approaches. In the first approach, Connectivity Map (CMAP) analysis of these TFs and their target networks outperformed standard approaches, retrieving ATRA as the top hit. We identify dimaprit and mebendazole as a drug combination which induces myeloid differentiation. In the second approach, we show that genetic manipulation of specific Mogrify ®-identified TFs (MYC and IRF1) leads to co-operative induction of APL differentiation, as does pharmacological targeting of these TFs using currently available compounds. We also show that loss of IRF1 blunts ATRA-mediated differentiation, and that MYC represses IRF1 expression through recruitment of PML-RARα, the driver fusion oncoprotein in APL, to the IRF1 promoter. Finally, we demonstrate that these drug combinations can also induce differentiation of primary patient-derived APL cells, and highlight the potential of targeting MYC and IRF1 in high-risk APL. Thus, these results suggest that Mogrify ® could be used for drug discovery or repositioning in leukaemia differentiation therapy for other subtypes of leukaemia or cancers.
Published: 2022

41. The strategic role of middle managers in the formulation and implementation of digital transformation projects

Author: Ioannis P. Christodoulou, Jahangir Wasim, Robert J. Reinhardt, and Kirill Ivanov
Subjects: General Business, Management and Accounting, Finance
Published: 2022

42. Evaluation of cyclodextrin‐ and cyclofructan‐based chiral selectors for the enantioseparation of psychoactive substances in capillary electrophoresis

Author: Katerina A. Ioannou, Atalanti Christou, Ioannis J. Stavrou, Martin G. Schmid, and Constantina P. Kapnissi‐Christodoulou
Subjects: Cyclodextrins, Amphetamine, Sulfates, Clinical Biochemistry, Electrophoresis, Capillary, Stereoisomerism, Biochemistry, Analytical Chemistry
Abstract: During this study, a simple and easy-to-prepare electrophoretic method was developed for the enantioseparation of amphetamine and cathinone derivatives. Different types of β-cyclodextrin and cyclofructan-based chiral selectors (CSs), both native and derivatized, were utilized, and the most effective ones, in terms of resolution and analysis time, were identified. In addition, several electrophoretic parameters, such as background electrolyte concentration and pH, and CS concentration, were examined to optimize the separation conditions. Under the optimal electrophoretic conditions, 10 psychoactive substances were enantiomerically separated using 1 mM sulfated cyclofructan-6 (SCF-6) for the amphetamine derivatives and 1 mM sulfated cyclofructan-7 (SCF-7) for the cathinone derivatives dissolved in an aqueous solution of 20-mM monobasic sodium phosphate at pH 2.5, a temperature of 25°C, and an applied voltage of 25 kV. In addition, the method was validated by estimating the intra- and interday precision.
Published: 2022

43. Dual Emission in the Near-Infrared and Visible Regions from a Mixed Cyanido-Bridged EuIII/NdIII(4-OHpy)-CoIII Layered Material

Author: Konstantinos Karachousos-Spiliotakopoulos, Vassilis Tangoulis, Anastasios Tasiopoulos, Nikos Panagiotou, Elefhteria Charalambous, Vassilis Nastopoulos, and Sotirios Christodoulou
Subjects: Inorganic Chemistry, Physical and Theoretical Chemistry
Published: 2022

44. Free‐breathing 3D CEST MRI of human liver at 3.0 T

Author: Pei Han, Karandeep Cheema, Tianle Cao, Hsu‐Lei Lee, Fei Han, Nan Wang, Hui Han, Yibin Xie, Anthony G. Christodoulou, and Debiao Li
Subjects: Imaging, Three-Dimensional, Liver, Humans, Radiology, Nuclear Medicine and imaging, Protons, Magnetic Resonance Imaging, Amides
Abstract: To develop a novel 3D abdominal CEST MRI technique at 3 T using MR multitasking, which enables entire-liver coverage with free-breathing acquisition.k-Space data were continuously acquired with repetitive steady-state CEST (ss-CEST) modules. The stack-of-stars acquisition pattern was used for k-space sampling. MR multitasking was used to reconstruct motion-resolved 3D CEST images of 53 frequency offsets with entire-liver coverage and 2.0 × 2.0 × 6.0 mmBoth APT-CEST and glycoCEST signals showed high sensitivity between post-fasting and post-meal acquisitions. APT-CEST and glycoCEST MTRasym signals from post-mean scans were significantly increased (APT-CEST: -0.019 ± 0.017 in post-fasting scans, 0.014 ± 0.021 in post-meal scans, p 0.01; glycoCEST: 0.003 ± 0.009 in post-fasting scans, 0.027 ± 0.021 in post-meal scans, p 0.01).The proposed 3D abdominal steady-state CEST method using MR multitasking can generate CEST images of the entire liver during free breathing.
Published: 2022

45. <scp>MR</scp> multitasking‐based dynamic imaging for cerebrovascular evaluation ( <scp>MT‐DICE</scp> ): Simultaneous quantification of permeability and leakage‐insensitive perfusion by dynamic T1/T2* mapping

Author: Zhehao Hu, Anthony G. Christodoulou, Nan Wang, Yibin Xie, Mark S. Shiroishi, Wensha Yang, Gabriel Zada, Frances E. Chow, Ashley S. Margol, Benita Tamrazi, Eric L. Chang, Debiao Li, and Zhaoyang Fan
Subjects: Radiology, Nuclear Medicine and imaging
Published: 2022

46. Mathematical Modeling and Optimization to Inform Impurity Control in an Industrial Active Pharmaceutical Ingredient Manufacturing Process

Author: Samir Diab, Charalampos Christodoulou, George Taylor, and Philip Rushworth
Subjects: Organic Chemistry, Physical and Theoretical Chemistry
Published: 2022

47. MENTAL HEALTH PROMOTION

Author: George N Christodoulou
Abstract: Background: Having the person at the center of health, both in person-centered clinical care and people-centered public health [1] are a guiding principle in health promotion. Mental health is an integral part of each person’s health and well-being. This is an absolute necessity always and more so during the years of the coronavirus pandemic and the post-pandemic “new normal”. Objective: To discuss the basic concepts of health, mental health, health promotion and mental health promotion, focusing on peace and justice, two key target areas and goals of mental health promotion [2,3]. Method: The holistic approach, the need to reduce stigma and discrimination, and the person- and people-centered approach in mental health promotion were reviewed. Results: The importance of identifying target populations and using paradigms of mental health promotion initiatives, like the Athens Mental Health Promotion Initiative, was noted, as well as the positive role played by celebrities who have excelled despite their mental disorders (“recovery”). The relevance of mental health promotion guidance and effectiveness of existing mental health promotion strategies have to be updated by further research in order to meet the need for intensification of mental health promotion efforts during the COVID pandemic. Conclusion: In the post-COVID era a “new normal” requires an update of policies for an intensified and different person-centered approach delivered by different providers to different recipients of services.
Published: 2022

48. Biallelic pathogenic variants in COX11 are associated with an infantile‐onset mitochondrial encephalopathy

Author: Rocio Rius, Neal K. Bennett, Kaustuv Bhattacharya, Lisa G. Riley, Zafer Yüksel, Luke E. Formosa, Alison G. Compton, Russell C. Dale, Mark J. Cowley, Velimir Gayevskiy, Saeed M. Al Tala, Abdulrahman A. Almehery, Michael T. Ryan, David R. Thorburn, Ken Nakamura, and John Christodoulou
Subjects: Genetics, Genetics (clinical)
Abstract: Primary mitochondrial diseases are a group of genetically and clinically heterogeneous disorders resulting from oxidative phosphorylation (OXPHOS) defects. COX11 encodes a copper chaperone that participates in the assembly of complex IV and has not been previously linked to human disease. In a previous study, we identified that COX11 knockdown decreased cellular adenosine triphosphate (ATP) derived from respiration, and that ATP levels could be restored with coenzyme Q
Published: 2022

49. Degradation Mechanism of Metal–Organic Framework Drug Nanocarriers Studied by Solid-State Nuclear Magnetic Resonance and X-ray Absorption Near-Edge Structure Spectroscopy

Author: Mai Dang Le Vuong, Ioanna Christodoulou, Marianna Porcino, Si-Thanh Dong, Benedikt Lassalle-Kaiser, Mohamed Haouas, Ruxandra Gref, and Charlotte Martineau-Corcos
Subjects: General Chemical Engineering, Materials Chemistry, General Chemistry
Published: 2022

50. The effect of preharvest applications with biostimulants on qualitative properties and postharvest performance of loquat fruit

Author: Pavlou, Andrea, Efstathiou, E., Christodoulou, S. E., Stylianou, S., and Manganaris, George A.
Subjects: Biostimulants, Eriobotrya japonica, Agricultural Sciences, Horticulture, Sugars, Acids, Yield efficiency, Developmental stages
Abstract: Loquat (Eriobotrya japonica L.) cultivation is one of the most promising crops under sub-tropical areas of the Mediterranean basin due to the fact that fruit ripening occurs in a period when there is no competition from other fresh fruits. The present work studied the effect of commercial formulations of biostimulants on qualitative attributes and postharvest performance of loquat fruit. Fifteen trees of ‘Karantoki’ with similar vigour from a commercial orchard were segregated into five groups of three trees and received the following treatments: 1) control, 2) kelp, 3) Folicist, 4) Fylloton, and 5) a combination of Kriss and Sunred formulations at the dosages recommended by the manufacturers. The fruits of each group were harvested on three consecutive dates (H1, H2 and H3) and analyzed after maintenance at room temperature (shelf life) for 1 and 5 days, respectively. Notably, kelp formulation led to a significant advancement of on-tree ripening that is a desirable feature due to the higher prices the fruit receives on the market as an “off-season” product, as well as to a significant increment on fruit size. Furthermore, kelp- and Folicist-treated fruits were characterized by higher firmness retention and reduced weight loss compared to the rest treatments after shelf life periods. No beneficial effect was registered when Fylloton, Kriss and Sunred formulations were applied. The incorporation of biostimulants to the spray program of loquat trees need to be further dissected and accompanied by a cost/benefit analysis.
Published: 2022

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