34 results on '"Chinatsu Mori"'
Search Results
2. Impact of the COVID-19 Pandemic on the Management and End-of-life Care of Unresectable Pancreatic Cancer
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Akiyoshi Kasuga, Masanori Nojima, Takeshi Okamoto, Takahiro Ishitsuka, Manabu Yamada, Hiroki Nakagawa, Shohei Udagawa, Chinatsu Mori, Takafumi Mie, Takaaki Furukawa, Yuto Yamada, Tsuyoshi Takeda, Masato Matsuyama, Takashi Sasaki, Masato Ozaka, and Naoki Sasahira
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Internal Medicine ,General Medicine - Abstract
Objective The coronavirus disease (COVID-19) pandemic has altered the delivery of medical care. The present study evaluated the impact of COVID-19 on the outcomes of unresectable pancreatic cancer (PC) patients who received end-of-life care. Methods We retrospectively compared the management of PC patients during the COVID-19 pandemic (from April 2020 to March 2021) to the preceding year, which was unaffected by the pandemic (from April 2019 to March 2020), based on a prospectively maintained institutional database. Results A total of 178 patients were included in the COVID-19-exposed group and 201 patients were included in the COVID-19-unexposed group. The median overall survival was similar between the groups (exposed vs. unexposed: 12.6 vs. 11.9 months, p = 0.174). Treatment regimens and relative dose intensities and the progression-free survival of GnP (gemcitabine in combination with nab-paclitaxel) and mFOLFIRINOX as first- and second-line chemotherapy did not differ significantly between the two groups. Only 9.0% of patients died at home in the COVID-19-unexposed group, compared to 32.0% in the COVID-19-exposed group (p0.001). A multivariate analysis revealed that death during the COVID-19 exposed period was independently associated with home death (odds ratio: 4.536, 95% confidence interval: 2.527-8.140, p0.001). Conclusions While the COVID-19 pandemic did not seem to influence chemotherapeutic treatment for PC patients at our institution, it had a large impact on end-of-life care. These findings may promote discussion about end-of-life care in Japan.
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- 2022
3. Effect of alcohol on productivity and quality of adeno-associated virus 2 in HEK293 cells
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Shunsuke Shiina, Nobuyoshi Nagao, Junichi Hasegawa, Tori Sato, Chinatsu Mori, Kaya Ohtaki, Kiyomi Kubodera, Yuri Yamashita, Kana Tanabe, Yasuhiro Kawano, and Hideki Aoyagi
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HEK293 Cells ,Ethanol ,Proteome ,Genetic Vectors ,Humans ,Bioengineering ,Dependovirus ,Kidney ,Applied Microbiology and Biotechnology ,Biotechnology - Abstract
Investigation of enhancers to improve recombinant adeno-associated virus 2 (rAAV2) productivity by human embryonic kidney 293 cells (HEK293) suspension culture showed that the addition of ethanol improved the productivity and packaged genome integrity of rAAV2. Further optimization showed that adding ethanol in the range of 0.09%-1.11% (v/v) during rAAV2 production effectively improved rAAV2 productivity and quality. In addition, ethanol addition improved cell viability. Furthermore, proteome and pathway analysis of the cells during rAAV2 production showed that the addition of ethanol resulted in the upregulation of pathways related to intercellular signaling, gene expression, cell morphology, intercellular maintenance, and others. In contrast, pathways related to cell death, immunity, and reactions to infection were downregulated. These changes in pathway regulation were responsible for the improvement in rAAV2 productivity, packaged genome integrity, and cell viability during rAAV2 production. The results of this study can be applied to the production of viral vectors for in vivo gene therapy in an inexpensive and safe manner.
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- 2022
4. Diagnostic Yield of Serial Pancreatic Juice Aspiration Cytologic Examination With Brush Cytology for Pancreatic Ductal Stenosis
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Takafumi Mie, Takashi Sasaki, Tsuyoshi Takeda, Takeshi Okamoto, Chinatsu Mori, Takaaki Furukawa, Yuto Yamada, Akiyoshi Kasuga, Masato Matsuyama, Masato Ozaka, and Naoki Sasahira
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Endocrinology ,Hepatology ,Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
Serial pancreatic-juice aspiration cytologic examination (SPACE) is useful for diagnosis of pancreatic ductal stenosis. This study investigates the utility of adding brush cytology to SPACE during the same procedure.We retrospectively analyzed consecutive patients who underwent SPACE with brush cytology for pancreatic ductal stenosis between February 2014 and July 2020 in our hospital.Thirty-four patients were included. Eleven had lesions in the pancreatic head lesions, and 23 had lesions in the pancreatic body or tail. Malignancies were found in 22 patients. Endoscopic ultrasound, computed tomography, and magnetic resonance imaging showed distal pancreatic duct dilation in 81.8% to 90.6% of cases, with a sensitivity of 63.0% to 65.5%. The sensitivity and diagnostic accuracy of SPACE, brush cytology, and SPACE with brush cytology were 63.6%, 50.0%, and 77.3% (P = 0.19) and 73.5%, 67.6%, and 82.4% (P = 0.42), respectively. No significant differences in diagnostic yield were observed for either pancreatic head lesions or pancreatic body/tail lesions. Post-endoscopic retrograde cholangiopancreatography pancreatitis was observed in 4 cases (11.8%).The utility of adding brush cytology to SPACE was limited.
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- 2022
5. Risk factors for recurrent stenosis after balloon dilation for benign hepaticojejunostomy anastomotic stricture
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Takafumi Mie, Takashi Sasaki, Takeshi Okamoto, Tsuyoshi Takeda, Chinatsu Mori, Yuto Yamada, Takaaki Furukawa, Akiyoshi Kasuga, Masato Matsuyama, Masato Ozaka, and Naoki Sasahira
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Gastroenterology ,Medicine (miscellaneous) ,Radiology, Nuclear Medicine and imaging - Published
- 2023
6. Long‐term outcomes of endoscopic double stenting using an anti‐reflux metal stent for combined malignant biliary and duodenal obstruction
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Takashi Sasaki, Tsuyoshi Takeda, Yuto Yamada, Takeshi Okamoto, Chinatsu Mori, Takafumi Mie, Akiyoshi Kasuga, Masato Matsuyama, Masato Ozaka, and Naoki Sasahira
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Hepatology ,Surgery - Abstract
To evaluate long-term outcomes of endoscopic double stenting using anti-reflux metal stents (ARMS) for combined malignant biliary and duodenal obstruction.Consecutive patients with advanced pancreatic cancer who received endoscopic double stenting with self-expandable metal stents (SEMS) for combined malignant biliary and duodenal obstruction at our institution between July 2014 and March 2021 were evaluated. Patients were divided into the ARMS group, endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) group, and covered metal stent-transpapillary (CMS-transpapillary) group. A Duckbill-type metal stent was used in all ARMS cases.Thirty-eight patients were enrolled: ARMS group (n = 16), EUS-HGS group (n = 13), and CMS-transpapillary group (n = 9). Overall survival among three groups were not significantly different. Recurrent biliary obstruction (RBO) rates of the ARMS, EUS-HGS, and CMS-transpapillary groups were 12.5%, 61.5%, and 88.9% (P .01) and median time to recurrent biliary obstructions (TRBOs) were not reached, 125 days, and 7 days (P .01). Median TRBOs of ARMS-choledochoduodenostomy and ARMS-transpapillary were not statistically different. Major causes of RBO were stent occlusion and symptomatic stent migration in the ARMS group, hyperplasia in the EUS-HGS group, and non-occlusion cholangitis in the CMS-transpapillary group.Endoscopic double stenting with ARMS might be an option for combined malignant biliary and duodenal obstruction.
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- 2022
7. Rotatable sphincterotome as a rescue device for endoscopic retrograde cholangiopancreatography cannulation: a single-center experience
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Takeshi Okamoto, Takashi Sasaki, Tsuyoshi Takeda, Takafumi Mie, Chinatsu Mori, Takaaki Furukawa, Yuto Yamada, Akiyoshi Kasuga, Masato Matsuyama, Masato Ozaka, and Naoki Sasahira
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Gastroenterology ,Medicine (miscellaneous) ,Radiology, Nuclear Medicine and imaging - Published
- 2023
8. Pancreatic follicular lymphoma: a report of two cases and literature review
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Chinatsu Mori, Masato Matsuyama, Manabu Takamatsu, Takafumi Mie, Noriko Nishimura, Takaaki Furukawa, Takashi Sasaki, Dai Maruyama, Naoki Sasahira, Akiyoshi Kasuga, Tsuyoshi Takeda, Yuto Yamada, Masato Ozaka, and Takeshi Okamoto
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Monoclonal antibody ,Male ,Bendamustine ,medicine.medical_specialty ,Follicular lymphoma ,Case Report ,Gastroenterology ,chemistry.chemical_compound ,Maintenance therapy ,immune system diseases ,Surgical oncology ,Obinutuzumab ,Endoscopic ultrasound-guided fine-needle aspiration ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,Pancreas ,Endoscopic Ultrasound-Guided Fine Needle Aspiration ,Lymphoma, Follicular ,Aged ,business.industry ,Malignant lymphoma ,Lymphoma, Non-Hodgkin ,General Medicine ,Hepatology ,medicine.disease ,Lymphoma ,Pancreatic Neoplasms ,medicine.anatomical_structure ,chemistry ,business ,medicine.drug - Abstract
Primary pancreatic lymphomas are extremely rare, accounting for 0.1-0.5% of malignant lymphomas and about 0.2% of primary pancreatic tumors. They occur most commonly in the pancreatic head of elderly males, with diffuse large B-cell lymphoma being the most common subtype. Primary pancreatic follicular lymphoma is the most commonly reported primary pancreatic indolent lymphoma, accounting for 9-14% of primary pancreatic lymphomas. We report two cases of primary pancreatic follicular lymphoma treated with obinutuzumab, a second-generation humanized anti-CD20 monoclonal antibody, and bendamustine. One was diagnosed by endoscopic ultrasound-guided fine-needle aspiration, while the other required laparoscopic lymph node sampling to reach a diagnosis. Both achieved complete response with induction therapy and opted for maintenance therapy with obinutuzumab. We also conducted a literature review of primary pancreatic follicular lymphoma cases reported over the last 30 years.
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- 2021
9. Molecular basis for thermal stability and affinity in a VHH: Contribution of the framework region and its influence in the conformation of the CDR3
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Seisho Kinoshita, Makoto Nakakido, Chinatsu Mori, Daisuke Kuroda, Jose M.M. Caaveiro, and Kouhei Tsumoto
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Molecular Conformation ,Amino Acid Sequence ,Single-Domain Antibodies ,Molecular Biology ,Biochemistry ,Complementarity Determining Regions - Abstract
The camelid single domain antibody, referred to VHH or Nanobody, is considered a versatile tool for various biotechnological and clinical applications because of its favorable biophysical properties. To take advantage of these characteristics and for its application in biotechnology and therapy, research on VHH engineering is currently vigorously conducted. To humanize a camelid VHH, we performed complementarity determining region (CDR) grafting using a humanized VHH currently in clinical trials, and investigated the effects of these changes on the biophysical properties of the resulting VHH. The chimeric VHH exhibited a significant decrease in affinity and thermal stability and a large conformational change in the CDR3. To elucidate the molecular basis for these changes, we performed mutational analyses on the framework regions revealing the contribution of individual residues within the framework region. It is demonstrated that the mutations resulted in the loss of affinity and lower thermal stability, revealing the significance of bulky residues in the vicinity of the CDR3, and the importance of intramolecular interactions between the CDR3 and the framework-2 region. Subsequently, we performed back-mutational analyses on the chimeric VHH. Back-mutations resulted in an increase of the thermal stability and affinity. These data suggested that back-mutations restored the intramolecular interactions, and proper positioning and/or dynamics of the CDR3, resulting in the gain of thermal stability and affinity. These observations revealed the molecular contribution of the framework region on VHHs and further designability of the framework region of VHHs without modifying the CDRs.
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- 2022
10. Treatment outcomes of erlotinib plus gemcitabine as late-line chemotherapy in unresectable pancreatic cancer
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Chinatsu Mori, Masato Ozaka, Naoki Sasahira, Takashi Sasaki, Yuto Yamada, Takeshi Okamoto, Masato Matsuyama, Takafumi Mie, Tsuyoshi Takeda, Akiyoshi Kasuga, and Takaaki Furukawa
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0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,FOLFIRINOX ,medicine.medical_treatment ,Deoxycytidine ,Erlotinib Hydrochloride ,03 medical and health sciences ,0302 clinical medicine ,Carcinoembryonic antigen ,Internal medicine ,Pancreatic cancer ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Retrospective Studies ,Chemotherapy ,Performance status ,biology ,business.industry ,General Medicine ,medicine.disease ,Gemcitabine ,Chemotherapy regimen ,Pancreatic Neoplasms ,Treatment Outcome ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Erlotinib ,business ,medicine.drug - Abstract
Objective With the introduction of modified FOLFIRINOX and gemcitabine plus nab-paclitaxel therapy for unresectable pancreatic cancer, erlotinib plus gemcitabine therapy is now occasionally used as late-line therapy. This study investigates outcomes of treatment with erlotinib plus gemcitabine for unresectable pancreatic cancer. Methods We retrospectively analysed consecutive patients with unresectable pancreatic cancer treated with erlotinib plus gemcitabine as the third or later-line chemotherapy between March 2014 and December 2020 in our hospital. Results A total of 56 patients were included (third line/fourth or later line = 42/14). All patients were previously treated with gemcitabine plus nab-paclitaxel and 45 patients were previously treated with modified FOLFIRINOX. The median progression-free survival (PFS) and overall survival (OS) were 1.6 and 4.6 months, respectively. The disease control rate was 21.4%. Performance status, modified Glasgow prognostic score and carcinoembryonic antigen level were independently associated with survival. Our prognostic model using these parameters could classify patients into good (n = 32) and poor (n = 24) prognostic groups. The median PFS and OS were longer in good than in poor prognostic group, but the difference in PFS was very small (PFS: 2.1 vs. 1.4 months, P = 0.01. OS: 6.8 vs. 2.4 months, P < 0.01). Interstitial pneumonia occurred in one patient (1.8%). Conclusions Benefits of erlotinib plus gemcitabine as late-line chemotherapy were limited, particularly with respect to PFS. Development of more effective third-line treatment options is desirable in the future.
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- 2021
11. Biophysical insight into protein-protein interactions in the Interleukin-11/Interleukin-11Rα/glycoprotein 130 signaling complex
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Chinatsu Mori, Satoru Nagatoishi, Ryo Matsunaga, Daisuke Kuroda, Makoto Nakakido, and Kouhei Tsumoto
- Abstract
Interleukin-11 (IL-11) is a member of the interleukin-6 (IL-6) family of cytokines. IL-11 is a regulator of multiple events in hematopoiesis, and IL-11-mediated signaling is implicated in inflammatory disease, cancer, and fibrosis. All IL-6 family cytokines signal through the signal-transducing receptor, glycoprotein 130 (gp130), but these cytokines have distinct as well as overlapping biological functions. To understand IL-11 signaling at the molecular level, we performed a comprehensive interaction analysis of the IL-11 signaling complex, comparing it with the IL-6 complex, one of the best-characterized cytokine complexes. Our thermodynamic analysis revealed a clear difference between IL-11 and IL-6. Surface plasmon resonance analysis showed that the interaction between IL-11 and IL-11 receptor α (IL-11Rα) is entropy driven, whereas that between IL-6 and IL-6 receptor α (IL-6Rα) is enthalpy driven. Our analysis using isothermal titration calorimetry revealed that the binding of gp130 to the IL-11/IL-11Rα complex results in entropy loss, but that the interaction of gp130 with the IL-6/IL-6Rα complex results in entropy gain. Our hydrogen-deuterium exchange mass spectrometry experiments suggested that the D2 domain of gp130 was not involved in n IL-6-like interactions in the IL-11/IL-11Rα complex. It has been reported that IL-6 interaction with gp130 in the signaling complex was characterized through the hydrophobic interface located in its D2 domain of gp130. Our findings suggest that unique interactions of the IL-11 signaling complex with gp130 are responsible for the distinct biological activities of IL-11 compared to IL-6.
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- 2022
12. Multiple Gastric Metastases after Distal Pancreatectomy for Pancreatic Cancer
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Yuto Yamada, Takashi Sasaki, Tsuyoshi Takeda, Takeshi Okamoto, Manabu Takamatsu, Chinatsu Mori, Takafumi Mie, Takaaki Furukawa, Akiyoshi Kasuga, Masato Matsuyama, Masato Ozaka, Yoshinori Igarashi, and Naoki Sasahira
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Pancreatic Neoplasms ,Lung Neoplasms ,Pancreatectomy ,Stomach ,Internal Medicine ,Humans ,Female ,General Medicine ,Aged - Abstract
A 67-year-old woman underwent distal pancreatectomy for pancreatic cancer. Recurrence in the form of lung metastasis was discovered eight months after surgery, and chemotherapy was initiated. Two years after the surgery, she was admitted for the evaluation of melena. Esophagogastroduodenoscopy revealed multiple subepithelial lesions with ulceration from the gastric body to the fornix. The histopathology of biopsy specimens was consistent with ductal adenocarcinoma, which appeared similar to the resected pancreatic cancer. The patient was diagnosed with multiple gastric metastases of pancreatic cancer. We herein report a case of pancreatic cancer with multiple gastric metastases that occurred after surgery for pancreatic tail cancer.
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- 2022
13. Early-onset pancreatic cancer: Clinical characteristics and survival outcomes
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Tsuyoshi Takeda, Takashi Sasaki, Yosuke Inoue, Takeshi Okamoto, Chinatsu Mori, Takafumi Mie, Takaaki Furukawa, Yuto Yamada, Akiyoshi Kasuga, Masato Matsuyama, Masato Ozaka, Yu Takahashi, Akio Saiura, and Naoki Sasahira
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Cohort Studies ,Pancreatic Neoplasms ,Hepatology ,Endocrinology, Diabetes and Metabolism ,Antineoplastic Combined Chemotherapy Protocols ,Smoking ,Gastroenterology ,Humans ,Neoadjuvant Therapy ,Retrospective Studies - Abstract
Early-onset pancreatic cancer (≤50 years, EOPC) is uncommon. This study aims to characterize the clinical and survival characteristics of EOPC in comparison to late-onset pancreatic cancer (50 years, LOPC).We retrospectively investigated consecutive PC patients treated at our institution between 2010 and 2019. We analyzed and compared clinicopathological characteristics, treatments, and outcomes of EOPC and LOPC.Of 1646 PC patients identified (768 resectable/borderline resectable; 248 locally advanced; 630 metastatic), 127 (8%) had EOPC. Current smoking and heavy drinking were associated with EOPC. EOPC presented at a more advanced stage and had higher neutrophil-to-lymphocyte ratios than LOPC. Survival outcomes were similar between the two groups, both in the entire cohort and in each resectability group. In patients undergoing resection, EOPC tended to have a higher N stage (p = 0.099) and had a higher pathological stage (stage IV, 20% vs. 7%, p = 0.005) and a lower rate of macroscopically curative resection (80% vs. 93%, p = 0.006). Liver recurrence was more commonly observed in EOPC (42% vs. 23%, p = 0.015). In the metastatic cohort, combination chemotherapy regimens were more frequently administered in EOPC as first-line treatment (79% vs. 64%, p = 0.028). Both median PFS (4.4 vs. 5.3 months, p = 0.647) and OS (11.5 vs. 9.5 months, p = 0.183) were not significantly different between the two groups.EOPC presented with a more aggressive tumor biology. Survival outcomes were similar to LOPC due to more intensive treatment.
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- 2022
14. A case of necrotic enteritis during neoadjuvant chemotherapy with gemcitabine and S-1 for resectable pancreatic ductal adenocarcinoma
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Naoki Sasahira, Fumihiro Kawano, Takaaki Furukawa, Manabu Takamatsu, Akiyoshi Kasuga, Takeshi Okamoto, Takashi Sasaki, Masato Matsuyama, Takafumi Mie, Masato Ozaka, Yoshihiro Ono, Yuto Yamada, Chinatsu Mori, Tsuyoshi Takeda, and Yu Takahashi
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Male ,medicine.medical_specialty ,medicine.medical_treatment ,Adenocarcinoma ,Gastroenterology ,Deoxycytidine ,Enteritis ,Dihydropyrimidine dehydrogenase deficiency ,Surgical oncology ,Internal medicine ,Pancreatic cancer ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Aged ,Chemotherapy ,business.industry ,General Medicine ,Hepatology ,Middle Aged ,medicine.disease ,Gemcitabine ,Colorectal surgery ,Neoadjuvant Therapy ,Pancreatic Neoplasms ,business ,medicine.drug - Abstract
Pancreatic cancer is the fourth leading cause of cancer-related death in Japan. Pancreatic cancer is categorized as resectable, borderline resectable, or unresectable based on the degree of adjacent vascular invasion and the presence of distant metastases. Neoadjuvant chemotherapy with gemcitabine and S-1 (NAC-GS) has recently become a standard option for resectable pancreatic cancer in Japanese patients. According to previous reports, GS is considered to be relatively safe and feasible treatment for Japanese patients, including the elderly. However, NAC-GS is occasionally associated with severe adverse events which may ultimately render the patient unfit for surgery. A 60-year-old man with resectable pancreatic cancer suffered from severe necrotic enteritis during NAC-GS, which required surgical resection. Considering the time course and histological findings of the resected bowel, S-1 was believed to be the causative agent. The low urinary dihydrouracil to uracil ratio also suggested possible dihydropyrimidine dehydrogenase deficiency, which may have hindered the metabolism of S-1 and contributed to the development of necrotic enteritis. Life-threatening enteritis occurs in approximately 0.3% of all patients who receive S-1. As initial symptoms are non-specific, patients should be instructed to lower the hurdle for contacting the hospital during NAC-GS.
- Published
- 2021
15. Application of nozzleless electrostatic atomization to encapsulate soybean oil with solid substances
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Atsuko Shimosaka, Yoshiyuki Shirakawa, Chinatsu Mori, Kazunori Kadota, Mikio Yoshida, and Yuichi Tozuka
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chemistry.chemical_classification ,Materials science ,food.ingredient ,Single process ,food and beverages ,04 agricultural and veterinary sciences ,040401 food science ,Wall material ,Soybean oil ,law.invention ,03 medical and health sciences ,0404 agricultural biotechnology ,0302 clinical medicine ,food ,Chemical engineering ,chemistry ,law ,Oil phase ,Emulsion ,030221 ophthalmology & optometry ,Crystallization ,Microscale chemistry ,Food Science ,Polyunsaturated fatty acid - Abstract
Many oil-encapsulation techniques, in which particles are used to improve the stability of polyunsaturated fatty acids (PUFAs) against oxidation and the handling of oils, have been reported. We developed techniques for encapsulating oil within powders with a liquid–liquid interfacial crystallization by using nozzleless electrostatic atomization. This process was used to prepare fine spherical encapsulated soybean oil particles with a microscale single process. An inexpensive soybean oil containing PUFAs was chosen as the oil phase. W/O emulsion systems were synthesized via the electrostatic atomization process. After the W/O emulsions were prepared, glycine and taurine well provided in supplements were used as the wall material for encapsulation. The soybean oil content of the encapsulated particles and their stability at high temperatures were evaluated. The oxidative stability of the soybean oil during high-temperature storage was improved for the encapsulation.
- Published
- 2019
16. P75-4 Nanoliposomal irinotecan plus 5-fluorouracil, leucovorin as post-gemcitabine-based therapy in pancreatic cancer
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Chinatsu Mori, Masato Ozaka, Takafumi Mie, Takaaki Furukawa, Yuto Yamada, Takeshi Okamoto, Tsuyoshi Takeda, Akiyoshi Kasuga, Masato Matsuyama, Takashi Sasaki, and Naoki Sasahira
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Oncology ,Hematology - Published
- 2022
17. Molecular Features and Clinical Management of Hereditary Pancreatic Cancer Syndromes and Familial Pancreatic Cancer
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Akiyoshi Kasuga, Takeshi Okamoto, Shohei Udagawa, Chinatsu Mori, Takafumi Mie, Takaaki Furukawa, Yuto Yamada, Tsuyoshi Takeda, Masato Matsuyama, Takashi Sasaki, Masato Ozaka, Arisa Ueki, and Naoki Sasahira
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QH301-705.5 ,hereditary pancreatic cancer syndrome ,familial pancreatic cancer ,Catalysis ,Inorganic Chemistry ,Neoplastic Syndromes, Hereditary ,Biomarkers, Tumor ,Humans ,Genetic Predisposition to Disease ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Germ-Line Mutation ,Spectroscopy ,Carcinoma ,Organic Chemistry ,Disease Management ,General Medicine ,Computer Science Applications ,Pancreatic Neoplasms ,Chemistry ,PARP inhibitor ,germline mutation ,surveillance ,loss of heterozygosity - Abstract
Hereditary pancreatic cancers are caused by several inherited genes. Familial pancreatic cancer is defined as pancreatic cancer arising in a patient with at least two first-degree relatives with pancreatic cancer in the absence of an identified genetic cause. Hereditary pancreatic cancer syndromes and familial pancreatic cancers account for about 10% of pancreatic cancer cases. Germline mutations in BRCA1, BRCA2, ATM, PALB2, CDKN2A, STK11, and TP53 and mismatch repair genes (MLH1, MSH2, MSH6, PMS2, and EPCAM) are among the well-known inherited susceptibility genes. Currently available targeted medications include poly (ADP-ribose) polymerase inhibitors (PARP) for cases with mutant BRCA and immune checkpoint inhibitors for cases with mismatch repair deficiency. Loss of heterozygosity of hereditary pancreatic cancer susceptibility genes such as BRCA1/2 plays a key role in carcinogenesis and sensitivity to PARP inhibitors. Signature 3 identified by whole genome sequencing is also associated with homologous recombination deficiency and sensitivity to targeted therapies. In this review, we summarize molecular features and treatments of hereditary pancreatic cancer syndromes and surveillance procedures for unaffected high-risk cases. We also review transgenic murine models to gain a better understanding of carcinogenesis in hereditary pancreatic cancer.
- Published
- 2022
18. 6-Hydroxydopamine induces secretion of PARK7/DJ-1 via autophagy-based unconventional secretory pathway
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Mayu Ando, T Yamamoto, Keito Konno, Noriko Noguchi, Shohei Yashirogi, Yasuomi Urano, Yoshiro Saito, Chinatsu Mori, and Ayano Fuji
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0301 basic medicine ,Protein Deglycase DJ-1 ,Biology ,medicine.disease_cause ,Mice ,03 medical and health sciences ,Autophagy ,medicine ,Animals ,Humans ,Secretion ,Oxidopamine ,Molecular Biology ,Cells, Cultured ,Secretory pathway ,Hydroxydopamine ,Secretory Pathway ,PARK7 ,AMPK ,Cell Biology ,Up-Regulation ,Cell biology ,Oxidative Stress ,HEK293 Cells ,030104 developmental biology ,Reactive Oxygen Species ,Oxidative stress ,Signal Transduction - Abstract
PARK7/DJ-1 is a Parkinson disease- and cancer-associated protein that functions as a multifunctional protein involved in gene transcription regulation and anti-oxidative defense. Although PARK7 lacks the secretory signal sequence, it is secreted and plays important physiological and pathophysiological roles. Whereas secretory proteins that lack the endoplasmic reticulum-targeting signal sequence are secreted from cells by way of what is called the unconventional secretion mechanism, the specific processes responsible for causing PARK7 to be secreted across the plasma membrane have remained unclear. In the present study, we found that PARK7 secretion was increased by treatment with 6-OHDA via the unconventional secretory pathway in human neuroblastoma SH-SY5Y cells and MEF cells. We also found that 6-OHDA-induced PARK7 secretion was suppressed in Atg5-, Atg9-, or Atg16l1-deficient MEF cells or ATG16L1 knockdown SH-SY5Y cells, indicating that the autophagy-based unconventional secretory pathway is involved in PARK7 secretion. We moreover observed that 6-OHDA-derived electrophilic quinone induced oxidative stress as indicated by a decrease in glutathione levels, and that this was suppressed by pretreatment with antioxidant NAC. We further found that NAC treatment suppressed autophagy and PARK7 secretion. We also observed that 6-OHDA-induced autophagy was associated with activation of AMPK and ULK1 via a pathway which was independent of MTOR. Collectively these results suggest that electrophilic 6-OHDA quinone enhances oxidative stress, and that this is followed by AMPK-ULK1 pathway activation and induction of secretory autophagy to produce unconventional secretion of PARK7.6-OHDA: 6-hydroxydopamine; AMPK: AMP-activated protein kinase; ATG: autophagy related; CAV1: caveolin 1; ER: endoplasmic reticulum; FN1: fibronectin 1; GSH: glutathione; IDE: insulin degrading enzyme; IL: interleukin; LDH: lactate dehydrogenase; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MEF: mouse embryonic fibroblast; MTOR: mechanistic target of rapamycin kinase; NAC: N-acetyl-L-cysteine; PARK7/DJ-1: Parkinsonism associated deglycase; PD: Parkinson disease; RPS6KB1/p70S6K: ribosomal protein S6 kinase B1; RPN1: ribophorin I; ROS: reactive oxygen species; ULK1: unc-51 like autophagy activating kinase 1; WT: wild-type.
- Published
- 2018
19. The effect of 'Hamamidori mat ' to improve growth environment of plants in coastal sandy land
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Satomi Okamura, Chinatsu Mori, Kenji Tanaka, and Hiroyuki Kumata
- Published
- 2017
20. A Powderization Process for Encapsulating with Functional Biomaterials Using Nozzleless Electrostatic Atomization
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Atsuko Shimosaka, Mikio Yoshida, Chinatsu Mori, Yoshiyuki Shirakawa, and Kazunori Kadota
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0303 health sciences ,food.ingredient ,Materials science ,030309 nutrition & dietetics ,Drug Compounding ,Static Electricity ,Biocompatible Materials ,04 agricultural and veterinary sciences ,040401 food science ,Wall material ,Soybean oil ,Taylor cone ,Soybean Oil ,03 medical and health sciences ,0404 agricultural biotechnology ,food ,Chemical engineering ,Oil content ,Emulsion ,Particle-size distribution ,Particle size ,Particle Size ,Powders ,Hydrophobic and Hydrophilic Interactions ,Food Science - Abstract
Powderization of oils has been used as a method to enhance the stability of polyunsaturated fatty acids. Previously, we successfully powderized soybean oil via nozzleless electrostatic atomization. The process of nozzleless electrostatic atomization process was applied to the one-step process of encapsulating oil in wall materials. The encapsulation of oils in powder is dependent on the wall materials. The present study aimed to resolve the behavior of oil encapsulated in particles using a novel method of electrostatic atomization, and to investigate the effect of wall materials on the oil content in the encapsulated formulations. The size of particles surrounding oil was dependent on the type of wall materials used for encapsulation, and the oil content within the encapsulation decreased with increase in particle size. Furthermore, wall materials with higher hydrophobicity increased the oil content within the encapsulation, as more hydrophobic particles could absorb the oil more effectively. PRACTICAL APPLICATION: Nozzleless electrostatic atomization is a new method for preparing encapsulation of oil using various wall materials.
- Published
- 2019
21. Real-time Nonlinear Signal Processing Super Resolution of 8K Endoscope Cameras
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Hiromasa Yamashita, Kenkichi Tanioka, Seiichi Gohshi, and Chinatsu Mori
- Subjects
8K resolution ,Endoscope ,Computer science ,business.industry ,Nonlinear signal processing ,Endoscopic surgery ,Computer vision ,Thread (computing) ,Artificial intelligence ,business ,Superresolution - Abstract
Presently, 8K is the highest resolution of video systems. Originally, 8K research started only for broadcasting services. However, aside from broadcasting, 8K resolution video systems have also an important application in medical field, and endoscopic surgery is in its practical stage. In endoscopic surgery, a 0.02 mm thread is used. This 0.02 mm thread is not visible when using a 4K endoscope. However, when an 8K endoscope is employed, the thread is visible; however, fine focus is still necessary. Moreover, adjusting the focus of 8K by using the common tools only, such as view finders or small monitors, is very difficult. Hence, commercial HD/4K cameras are equipped with auto-focus functions; however, the central areas are not always the focus points. The focus is very deep and the focus points change during endoscopic surgeries. Owing to these reasons, a surgeon should manually control the endoscope focus. It is always very difficult to adjust the focus accurately. Super resolution (SR) has been proposed to sharpen out-of-focus images. However, a real-time SR technology is necessary for the 8K endoscope. In this study, a nonlinear signal processing super resolution (NLSP) is introduced to improve the resolution of 8K endoscope cameras. NLSP can enhance the 8K endoscope images and improve the camera’s focus depth.
- Published
- 2019
22. Real-Time Noise Reduction Algorithm for Video with Non-linear FIR Filter
- Author
-
Chinatsu Mori and Seiichi Gohshi
- Subjects
Finite impulse response ,business.industry ,Computer science ,Image quality ,Noise reduction ,02 engineering and technology ,Noise ,Filter (video) ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,Computer vision ,Recursive filter ,Artificial intelligence ,business ,Infinite impulse response ,SIMPLE algorithm - Abstract
Noise is an essential issue for images and videos. Recently, a range of high-sensitivity imaging devices have become available. Cameras are often used under poor lighting conditions for security purposes or night time news gathering. Videos shot under poor lighting conditions are afflicted by significant noise which degrades the image quality. The process of noise removal from videos is called noise reduction (NR). Although many NR methods are proposed, they are complex and are proposed as computer simulations. In practical applications, NR processing of videos occurs in real-time. The practical real-time methods are limited and the complex NR methods cannot cope with real-time processing. Video has three dimensions: horizontal, vertical and temporal. Since the temporal relation is stronger than that of horizontal and vertical, the conventional real-time NR methods use the temporal infinite impulse response (IIR) filter to reduce noise. This approach is known as the inter-frame relation, and the noise reducer comprises a temporal recursive filter. Temporal recursive filters are widely used in digital TV sets to reduce the noise affecting images. Although the temporal recursive filter is a simple algorithm, moving objects leave trails when it reduces the high-level noise. In this paper, a novel NR algorithm is introduced. The proposed method uses finite impulse response (FIR) filter. The FIR filter does not suffer from this trail issue and shows better performance than NR using temporal recursive filters is proposed.
- Published
- 2019
23. Image Quality of a Smartphone Display with Super-Resolution
- Author
-
Chinatsu Mori and Seiichi Gohshi
- Subjects
Computer science ,Image quality ,business.industry ,Materials Science (miscellaneous) ,Computer vision ,Artificial intelligence ,business ,Superresolution - Published
- 2016
24. Mo1704 USEFULLNESS OF COLONIC STENTING FOR PALLIATIVE TREATMENT OF MALIGNANT COLONIC OBSTRUCTION
- Author
-
Maki Tobari, Chinatsu Mori, Motoyasu Kan, Takayoshi Nishino, and Tetsuya Hamano
- Subjects
Colonic obstruction ,medicine.medical_specialty ,Palliative treatment ,business.industry ,Internal medicine ,Gastroenterology ,medicine ,Radiology, Nuclear Medicine and imaging ,business - Published
- 2020
25. Subjective Assessment of Video Noise Reduction with Non-recursive Temporal Filter
- Author
-
Aya Kubota, Seiichi Gohshi, and Chinatsu Mori
- Subjects
010302 applied physics ,Signal processing ,Reducer ,Image quality ,business.industry ,Computer science ,media_common.quotation_subject ,020207 software engineering ,02 engineering and technology ,01 natural sciences ,Peak signal-to-noise ratio ,Reduction (complexity) ,Temporal filter ,0103 physical sciences ,0202 electrical engineering, electronic engineering, information engineering ,Contrast (vision) ,Computer vision ,Noise (video) ,Artificial intelligence ,business ,media_common - Abstract
Imaging devices always cause noise. In videos, a noise reducer (NR) can be used; however, a conventional NR also decreases resolution and recursive NRs cause blur and trailing in motion areas. Previously, one of the authors proposed a non-recursive NR algorithm; however, a quantitative comparison of the proposed and conventional NRs has not been performed. There are two methods to evaluate image quality. They are an objective assessment and a subjective assessment. Although the objective assessment, such as peak signal to noise ratio (PSNR) is easy to conduct, the assessment result does not always fit our feelings. In contrast, the subjective assessment requires observers, but the results are conforming to human vision. Thus, in this study, a subjective assessment was conducted to confirm the performance of the non-recursive NR. The results demonstrate that the proposed NR algorithm retains resolution and reduces noise.
- Published
- 2018
26. Real-Time Non-linear Noise Reduction Algorithm for Video
- Author
-
Chinatsu Mori and Seiichi Gohshi
- Subjects
Nonlinear system ,Computer science ,Noise reduction algorithm ,Algorithm - Published
- 2018
27. A Case of Curative Resection of Early α-Fetoprotein-Producing Gastric Cancer by Endoscopic Submucosal Dissection
- Author
-
Hidemi Unozawa, Kae Hashimoto, Yutaka Mitsunaga, Motoyasu Kan, Hideki Shinmura, Chinatsu Mori, Takashi Ooide, Tetusya Hamano, Maki Tobari, Takayoshi Nishino, and Osamu Goto
- Subjects
Curative resection ,medicine.medical_specialty ,business.industry ,Mechanical Engineering ,medicine ,Energy Engineering and Power Technology ,Cancer ,Endoscopic submucosal dissection ,Management Science and Operations Research ,medicine.disease ,business ,Surgery - Published
- 2018
28. Study on greening and zoning methods for bare lands by seed dispersal simulations
- Author
-
Tomoaki Ino, Chinatsu Mori, Ryoichi Ohno, Ryuji Nakajima, and Tsuyoshi Ishii
- Subjects
Greening ,Geography ,Agroforestry ,Seed dispersal ,Forestry ,Zoning - Published
- 2015
29. Subjective Assessment Method for Multiple Displays with and without Super Resolution
- Author
-
Chinatsu Mori and Seiichi Gohshi
- Subjects
business.industry ,Computer science ,Assessment methods ,Computer vision ,Artificial intelligence ,business ,Superresolution - Published
- 2017
30. Greening experiments of coastal dunes in Niigata by utilizing used growing-medium of mushrooms and humus
- Author
-
Tomiichi Yamada, Kazuki Mami, Kenji Tanaka, and Chinatsu Mori
- Subjects
Greening ,Environmental science ,Forestry ,Humus ,Coastal dunes - Published
- 2014
31. Super resolution image reconstruction and imaging device
- Author
-
Seiichi Gohshi, Kenkichi Tanioka, and Chinatsu Mori
- Subjects
Computer science ,business.industry ,020208 electrical & electronic engineering ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Super resolution image reconstruction ,Digital imaging ,Process (computing) ,02 engineering and technology ,Superresolution ,Signal ,Aliasing ,0202 electrical engineering, electronic engineering, information engineering ,Nyquist–Shannon sampling theorem ,020201 artificial intelligence & image processing ,Computer vision ,Artificial intelligence ,business - Abstract
Super resolution (SR) studies started in the 1990s and many papers were issued in the 2000s. Super resolution image reconstruction (SRR) is one of the most common SR methods. SRR reconstructs a high-resolution image (HRI) using multiple low-resolution images (LRIs). However, it requires that certain conditions are met for it to work based on the SRR algorithm. Aliasing in LRIs is one of the essential conditions for SRR. Aliasing occurs when the signal is sampled with specific frequencies not satisfying the Nyquist sampling theorem. In the beginning of this century, analog cameras were replaced with digital cameras. The digital cameras are equipped with digital imaging devices, such as complementary metal oxide semiconductors (CMOS), and charge-coupled devices (CCD), for imaging process. The imaging devices have imaging cells with finite sizes and areas. The cells generate aliasing in accordance with their fill factors. The ability of SRR is deeply related to the performance of imaging devices. In this paper, the relationship is discussed theoretically.
- Published
- 2016
32. Subjective assessment of super-resolution
- Author
-
Hirohisa Takeshita, Chinatsu Mori, Masaki Sugie, and Seiichi Gohshi
- Subjects
Assessment data ,Computer science ,business.industry ,Assessment methods ,Nonlinear signal processing ,Paired comparison ,Computer vision ,Artificial intelligence ,Scheffé's method ,business ,Superresolution - Abstract
Results of a subjective assessment of superresolution (SR) technology for 4K TV sets are reported. 4K TV sets are currently available in the market, and some are equipped with SR functionality. Recently, SR technology that uses nonlinear signal processing (NLSP) and can work in real time has been proposed. The proposed method can enhance images without enlargement. The subjective assessment was performed by comparing 4K videos with and without NLSP on 4K TV sets. The assessment method was a combination of Scheffe’s paired comparison and ITU-R BT.500. Assessment data were statistically analyzed, and the results proved that NLSP is superior in resolution.
- Published
- 2015
33. Efficient Total Synthesis of Bastadin 6, an anti-Angiogenic Brominated Tyrosine-Derived Metabolite from Marine Sponge
- Author
-
Atsushi Hiramatsu, Hiroaki Tsujita, Naoyuki Kotoku, Noriko Koizumi, Chinatsu Mori, and Motomasa Kobayashi
- Subjects
biology ,Chemistry ,Stereochemistry ,Metabolite ,Organic Chemistry ,Total synthesis ,General Medicine ,biology.organism_classification ,Biochemistry ,Sponge ,chemistry.chemical_compound ,Tetramer ,Ianthella basta ,Yield (chemistry) ,Drug Discovery ,Organic chemistry ,Oxidative coupling of methane ,Tyrosine - Abstract
An efficient total synthesis of bastadin 6 ( 1 ), a cyclic tetramer of brominated tyrosine derivatives from the marine sponge, Ianthella basta , with selective anti-angiogenic activity, was accomplished. We developed a novel Ce(IV)-mediated oxidative coupling reaction of 2,6-dibromophenols to give the diaryl ether derivatives, the characteristic segment of 1 . Condensation of two segments and subsequent intramolecular macrocyclization gave bastadin 6 ( 1 ) in nine steps, 26% overall yield.
- Published
- 2005
34. Study of Limitation of Super Resolution Image Reconstruction and Image Quality Assessment of Displays with Super Resolution[博士論文]
- Author
-
Chinatsu, Mori
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