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The powder diameter affects the heat generation and further influences the quality of weldments when the MZA powder is used as laser-absorbents to carry out laser transmission welding (LTW). It is difficult to measure the temperature due to its extremely short duration. A mathematical model considering the multiple reflections of metal powder is established to predict heat generation. The mathematical model is verified and then used to describe the influence of line energy density and powder diameter on the temperature profile of the LTW process. The results demonstrate that the temperature from experiments and simulation are consistent. Therefore, the established model is suitable to predict the heat generation of metal powder laser-absorbents in the LTW process.

In this paper, we explore the capabilities of a number of deep neural network models in generating whole-brain 3T-like MR images from clinical 1.5T MRIs. The models include a fully convolutional network (FCN) method and three state-of-the-art super-resolution solutions, ESPCN [26], SRGAN [17] and PRSR [7]. The FCN solution, U-Convert-Net, carries out mapping of 1.5T-to-3T slices through a U-Net-like architecture, with 3D neighborhood information integrated through a multi-view ensemble. The pros and cons of the models, as well the associated evaluation metrics, are measured with experiments and discussed in depth. To the best of our knowledge, this study is the first work to evaluate multiple deep learning solutions for whole-brain MRI conversion, as well as the first attempt to utilize FCN/U-Net-like structure for this purpose., Comment: Accepted to ICMLA 2022

Yu Wang,1,2,* Fenghui Wang,1,2,* Jing He,1,2,* Juan Du,1,2 Huahua Zhang,1,2 Haiyan Shi,1,2 Yani Chen,1,2 Yameng Wei,1,2 Wanjuan Xue,1,2 Jing Yan,1,2 Yun Feng,1,2 Yi Gao,1,2 Dan Li,1,2 Jiming Han,1,2 Jing Zhang1,2 1Department of Clinical Medicine, Medical College of Yan’an University, Yan’an, Shaanxi 716000, People’s Republic of China; 2Yan’an Key Laboratory of Chronic Disease Prevention and Research, Yan’an, Shaanxi 716000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing ZhangDepartment of Clinical Medicine, Medical College of Yan’an University, No. 38, Guanghua Road, Yan’an 716000, People’s Republic of ChinaTel +86-911-2650158Email yadxzj@163.comPurpose: This study was doneto investigate the inhibition effects of miR-30a-3p on mitotic arrest deficient 2 like 1 (MAD2L1) expression and the proliferation of gastric cancer cells.Patients and methods: Cluster analysis and the TCGA database were used to screen the key genes highly expressed in gastric cancer. Based on the LinkedOmics website, the correlation between the miR-30a-3p and the cell cycle-related target gene MAD2L1 in gastric cancer was analyzed. The mRNA and protein expression levels were detected with the quantitative real-time PCR and Western blot analysis. The cell proliferation and cell cycle were also detected and analyzed.Results: Bioinformatics analysis showed that MAD2L1 was highly expressed in tumor tissues compared with normal tissues. Compared with normal tissues, the miR-30a-3p was significantly decreased in the gastric cancer tissues. Moreover, MAD2L1 was significantly negatively correlated with the miR-30a-3p expression. Furthermore, over-expression of miR-30a-3p decreased the expression of MAD2L1 at the protein level, which inhibited the proliferation of AGS and BGC-823 gastric cancer cells. In addition, the cell cycles of AGS and BGC-823 cells were arrested at the G0/G1 phase.Conclusion: MAD2L1 is a pro-oncogene which is up-regulated in gastric cancer. The miR-30a-3p can down-regulate the MAD2L1 expression, inhibiting the proliferation of gastric cancer cells and affect the cell cycle.Keywords: gastric cancer, bioinformatics analysis, MAD2L1, miR-30a-3p, cell proliferation

Purpose miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. Methods Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-luciferase reporter assay was used to analyze the direct binding between miR-497-5p and 3ʹ-untranslated region of CBX4. Cell viability was measured with MTT assay. Flow cytometry was performed to detect cell cycle distribution. Results Here, using bioinformatics methods we firstly found that miR-497-5p regulated cervical carcinoma proliferation by targeting polycomb chromobox4 (CBX4). Expression of miR-497-5p in cervical carcinoma tissues was negatively correlated with CBX4. A binding region of miR-497-5p in 3ʹ-untranslated region of CBX4 was predicted. Further experiments confirmed that miR-497-5p directly targeted CBX4. Besides, RNA interference of CBX4 inhibited cervical cancer cell proliferation, arrested cells at S phase and reduced the expression of CDK2 and Cyclin A2 proteins. The use of miR-497-5p inhibitor compromised CBX4 interference RNAs induced cycle arrest of cervical cancer cells. Cells co-transfected with miR-497-5p inhibitors and CBX4 interference RNAs had a higher proliferation rate than CBX4 inference RNA-transfected cells. Conclusion All together, the present study demonstrates that miR-497-5p inhibits cervical cancer cells proliferation by directly targeting CBX4.

Yameng Wei,1,* Pengfei Liu,2,* Qian Li,3,* Juan Du,1 Yani Chen,1 Yu Wang,1 Haiyan Shi,1 Yanfeng Wang,1 Huahua Zhang,1 Wanjuan Xue,1 Yi Gao,1 Dan Li,1 Yun Feng,1 Jing Yan,1 Jiming Han,1 Jing Zhang11Department of Clinical Medicine, Medical College of Yan’an University, Yan’an 716000, People’s Republic of China; 2Department of Gastroenterology, Yan’an University Affiliated Hospital, Yan’an 716000, People’s Republic of China; 3Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, People’s Republic of China*These authors contributed equally to this workPurpose: Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a tetramethylfolate dehydrogenase enzyme involved in folate metabolism. The aim of this study is to determine the effect of MTHFD2 on the proliferation and metastasis of colorectal cancer (CRC).Patients and methods: MTHFD2 was silenced or overexpressed in CRC cells. qRT-PCR and Western blotting were used to analyze mRNA and protein expression, respectively. The MTT assay and colony forming assay were used to detect cell proliferation and colony formation ability. The cycle and apoptosis changes were detected by flow cytometry. Transwell experiments were used to analyze the migration ability of CRC cells.Results: The expression of MTHFD2 in 31 kinds of cancers was analyzed by bioinformatics, and MTHFD2 was found highly expressed in various cancer cells including CRC cells. Silencing the expression of MTHFD2 resulted in inhibition of the proliferation of CRC cells, weakening of the migration ability, blocking of the cell cycle in G0/G1-S phase, and promotion of the apoptosis of CRC cells. On the contrary, overexpression of MTHFD2 in CRC cells resulted in enhancement of the proliferation and migration ability, promotion of cell cycle progression and inhibition of cell apoptosis.Conclusion: MTHFD2 is positively related with colorectal cancer and the MTHFD2 gene is a tumor promoting gene in CRC cells.Keywords: methylenetetrahydrofolate dehydrogenase 2, colorectal cancer, oncogene

The role of the nucleotide-binding domain and leucine-rich repeat containing receptor NLRP10 in disease is incompletely understood. Using three separate mouse strains lacking the gene encoding NLRP10, only one of which had a coincidental mutation in DOCK8, we documented a role for NLRP10 as a suppressor of the cutaneous inflammatory response to Leishmania major infection. There was no evidence the enhanced local inflammation was due to enhanced inflammasome activity. NLRP10/DOCK8 dually deficient mice harbored lower parasite burdens at the cutaneous site of inoculation than wild-type controls, whereas singly NLRP10 deficient had similar parasites loads to controls, suggesting that DOCK8 promotes local growth of parasites in the skin whereas NLRP10 does not. NLRP10-deficient mice developed vigorous adaptive immune responses, indicating there was not a global defect in development of antigen specific cytokine production. Bone marrow chimeras showed the anti-inflammatory role of NLRP10 was mediated by NLRP10 expressed in resident cells in the skin, rather than bone marrow-derived cells. These data suggest a novel role for NLRP10 in the resolution of local inflammatory responses during L. major infection.

Correction for ‘Enabling room-temperature processed highly efficient and stable 2D Ruddlesden–Popper perovskite solar cells with eliminated hysteresis by synergistic exploitation of additives and solvents’ by Shuang Yu et al., J. Mater. Chem. A, 2019, 7, 2015–2021.

In the frame of the intense research on electronics beyond CMOS, molecular electronics offers the versatility of organic chemistry in order to tailor new functions combining optical and electronic specifications, while accessing the quantum confined regime intrinsic to small molecules. As 1D conductors, carbon nanotubes bridge the gap between small molecules electronics and semiconductor technology with great promises while being a playground for organic chemistry. Beyond miniaturization, they offer the opportunity to design new devices from accurate sensors to optoelectronic and quantum devices. However most studies focus on sensor or photovoltaic applications and thus involve a macroscopic assembly of nanotubes. This averages the excitation transfers, which prevents access to their fundamental mechanisms. This requires the design of individual carbon nanotube based functional devices. For this issue double wall carbon nanotubes have many advantages over simple SWNTs. In general, they exhibit higher stability, which can be a substantial help in high-current and high-field experiments. They realize a core-shell system: their concentric structure suggests its use for independent doping or functionalization of inner and outer tubes.In this PhD project, we demonstrate field effect transistors based on hybrid systems of individual double wall carbon nanotubes and optically sensitive molecule.We first introduce the method for making individual double wall carbon nanotube field effect transistors (DWNT FETs), which are then characterized both optically and electrically. We also studied the electron phonon coupling in the DWNT system by Raman spectroscopy with electrostatic gating. The inner metallic tube is also affected by the electrostatic gate and shows dramatic changes of the overall Raman signature.We then functionalized non covalently two kinds of optically sensitive molecules to DWNT and graphene FETs (Terpyridine Osmium complex and Zinc(II) metalloporphyrin). The hybrids are characterized both optically and electrically. Charge transfer between DWNTs and molecules plays as a chemical gating which can be detected by Raman spectroscopy as well as electrical transport measurements, which indicates that the DWNT FETs can be utilized for molecular sensing. Light excitation of the molecules leads to doping of the hybrids and reveals the coupling between the nanotube walls.Moreover, we realized wavelength dependent optical gating on the hybrid device, detected by both Raman spectroscopy and electrical transport measurements at both room temperature and helium temperature. The optical control of the hybrids’ electronic behavior will be elucidated in terms of photo-induced charge transfer between the grafted molecules and the DWNT component. As a consequence, this hybrid FETs can be used as an optically controlled memory down to single electron transfers at low temperature., Dans le cadre de la recherche sur les dispositifs post-CMOS, l'électronique moléculaire bénéficie de la polyvalence de la chimie organique,qui offre de nouvelles fonctions alliant spécificités optiques et électroniques, tout en accédant au régime de confinement quantique intrinsèque aux petites molécules. Conducteurs 1D, les nanotubes de carbone font le lien entre l’électronique des petites molécules émergente et la technologie des semi-conducteurs, tout en tirant parti de la chimie organique. Au-delà de la miniaturisation, ils offrent la possibilité de concevoir de nouveaux dispositifs pour des capteurs, l’optoélectronique et l’électronique quantique. Cependant, la plupart des études se concentrent sur leurs applications aux capteurs ou pour le photovoltaïque qui impliquent un ensemble macroscopique de nanotubes. Dans ce cas, les transferts d'excitation sont moyennés sur un ensemble statistique, ce qui empêche l'accès à leurs mécanismes fondamentaux. Il est donc nécessaire de concevoir des dispositifs fonctionnels à base de nanotubes de carbone individuels. Pour cela, les nanotubes double paroi ont de nombreux avantages sur les monoparois. En général, ils présentent une stabilité plus élevée, qui peut être d’une aide substantielle dans des expériences à haute intensité et à fort champ. Ils réalisent un système cœur-coquille: leur structure concentrique suggère leur utilisation pour réaliser indépendamment un dopage ou une fonctionnalisation des tubes intérieur et extérieur.Dans ce projet de thèse, nous étudions des transistors à effet de champ basés sur des systèmes hybrides nanotubes individuels double paroi / chromophore.Nous présentons d'abord le procédé de fabrication de transistors à effet de champ de nanotubes de carbone à paroi individuels (DWFET), qui sont ensuite caractérisés à la fois par des techniques optiques et électriques. Nous avons notamment étudié le couplage électron-phonon par spectroscopie Raman sous dopage électrostatique. Le tube métallique interne apparaît également affecté par la grille électrostatique et montre des changements significatifs de la signature Raman.Nous avons ensuite fonctionnalisé les DWFETde façon non covalente avec deux types de molécules optiquement sensibles (terpyridine d'osmium et complexe de zinc (II) métalloporphyrine). Les hybrides sont caractérisés à la fois en optique et en transport électronique. Il apparaît un transfert de charge entre les molécules et le DWNT qui joue le rôle d’une grille chimique détectable par spectroscopie Raman et transport électrique, ce qui indique que les DWFET peuvent être utilisés pour la détection de molécules. L'excitation lumineuse des molécules conduit à un dopage des hybrides et permet de plus de révéler le couplage entre les parois des nanotubes.De plus, nous avons réalisé des expériences de grille optique à longueur d'onde variable sur les dispositifs hybrides, couplant à la fois la spectroscopie Raman et des mesures de transport électrique de la température ambiante jusqu’à la température de l'hélium. Le contrôle optique du comportement électronique des hybrides est expliqué en termes de transfert de charge photo-induit entre les molécules greffées et le DWNT. Par conséquent, nos FET hybrides peuvent être utilisés comme mémoire à commande optique jusqu’au régime de transfert d'électrons uniques.