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Additional file 1: Table S1. Patients’ liver type-fatty acid-binding protein immunohistochemical data and TNM state.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also known as coronavirus disease-2019 (COVID-19), is a pandemic disease that has been declared as modern history&rsquo, s gravest health emergency worldwide. Until now, no precise treatment modality has been developed. The angiotensin-converting enzyme 2 (ACE2) receptor, a host cell receptor, has been found to play a crucial role in virus cell entry, therefore, ACE2 blockers can be a potential target for anti-viral intervention. In this study, we evaluated the ACE2 inhibitory effects of 10 essential oils. Among them, geranium and lemon oils displayed significant ACE2 inhibitory effects in epithelial cells. In addition, immunoblotting and qPCR analysis also confirmed that geranium and lemon oils possess potent ACE2 inhibitory effects. Furthermore, the gas chromatography-mass spectrometry (GC&ndash, MS) analysis displayed 22 compounds in geranium oil and 9 compounds in lemon oil. Citronellol, geraniol, and neryl acetate were the major compounds of geranium oil and limonene that represented major compound of lemon oil. Next, we found that treatment with citronellol and limonene significantly downregulated ACE2 expression in epithelial cells. The results suggest that geranium and lemon essential oils and their derivative compounds are valuable natural anti-viral agents that may contribute to the prevention of the invasion of SARS-CoV-2/COVID-19 into the human body.

The 21st century is a generation of the knowledge economy that emphasizes technology and creativity. "Creativity" is seen as a new direction of education (Chen Longan, 1998; Shen Zhongwei, 2004). Time is slowly advancing. Nowadays, children receive education and accept the past. Multimedia teaching has added different novel teaching methods, such as video teaching and computer software-assisted teaching. When smart products are rapidly emerging, children are affected by the way these multimedia devices are affected.This study will use the school-age children in the scientific age as the research background, with the theme of "creative thinking and research", using Williams to create a power table, showing the innovative characteristics and causes of modern education. Suitable for school-age children. You can learn more about S-generation children to develop appropriate responses, such as job ability choices, weakness education guidance, and potential issues.

The QFD quality function is used to discuss the teaching of different courses in the display design field. Introducing the characteristics of quality function into teaching practice, emphasizing the need to consider customer needs from a professional level, transforming corresponding technological achievements, increasing understanding of consumer needs, improving design quality, achieving more realistic design practices, and replacing existing theories Courses to improve the effectiveness of the course teaching.In the Merchandising Presentation course, it is usually the theoretical design of the merchandise arrangement and display arrangement. However, there are many stores in the industry, the store style has begun to change differently, and the sales presentations have begun to have different definitions.Use the course to select your own product research. A relatively weak female accessory in the fashion industry is an example. In addition to high-priced jewelry, the accessories industry is often difficult to define value, consumers can only guess the value of goods from products and display forms. However, the display of accessories in hypermarkets, due to the variety of accessories, sales presentations often present a chaotic form, how to use sales demonstrations to stimulate the sale of budget women's accessories in hypermarkets as the focus of research.

Additional file 2: Figure S1. OPN induces EndoMT. A, mRNA levels of VE-cadherin, CD31, and α-SMA in the HUVECs pre-incubated 16 h with 2% FBS-containing M199 medium and then added with PBS or 0.3 μg/ml of OPN for further 24 h. We have previously reported that OPN induced EndoMT of HUVECs and immortalized endothelial cell line EC-RF24 (12). Consistently, the present data shows that OPN induced down-regulation of endothelial cell marker genes (VE-cadherin and CD31) but up-regulation of mesenchymal cell marker gene α-SMA. B, mRNA levels of VE-cadherin, Tie1, Tie2, CD31, α-SMA, and fibronectin in mouse immortalized endothelial cell line 3B-11 pre-incubated 16 h with 1% FBS-containing RPMI 1640 medium and then added with PBS or 0.3 μg/ml of OPN for further 24 h. The data revealed that OPN also induced EndoMT of 3B-11 cells. Figure S2. Using TGF-β-induced EndoMT model to confirm EndoMT CM-induced marophage M2-polarization. EndoMT CM and control medium (CTRL) were prepared as described in the Methods section except 20 ng/ml of TGF-β was used instead of OPN. THP-1-derived macrophages were treated with CTRL or EndoMT CM for 24 h. Relative mRNA levels of IL-1β, TNF-α, iNOS, CD163, CD204, IL-10, TGF-β, and Arg1 were assessed by qPCR analyses. #, P < 0.001 when compared with CTRL. Figure S3. eHSP90α enhances the physical association of CD91 with TLR4. PLAs showed red fluorescent dots in PBS or rHSP90α-treated macrophages by using the antibody combination detecting the physical interaction of CD91–TLR4. The level of red fluorescent dots was increased upon rHSP90α treatment.

Additional file 1: Table S1. List of the antibodies used in this study. Table S2. The primers and PCR conditions adopted in this study. All reactions started at 95°C for 5 min and terminated at 72°C for 7 min.

This study utilizes the Taiwan colonial household registration data to study the cause of uxorilocal marriage in Taiwan during the period of Japanese colonial rule and, simultaneously, to compare the prevalence of uxorilocal marriage in urban (Taipei) and rural (XinChu) communities. The socioeconomic status of the bride and groom’s family, and ethnic and urban–rural differences are the main issues in this research. In addition, the viewpoints of Wolf and Huang and Pasternak will be rechecked vis-à-vis regional differences. The results of this study will be presented by statistic analysis and geographic information system. In Taiwan, compared to major and minor marriage, uxorilocal marriage had a higher ratio in Taipei and XinChu. In uxorilocal marriage, the age of brides and grooms is older in Taipei than in XinChu. In addition, our research indicates either daughters or adopted daughters, if they had fewer siblings or no brothers, had a higher probability of entering into uxorilocal marriages. Moreover, uxorilocal marriages were found to be less prevalent in later birth cohorts while ethnic groups and parent’s marriage types had no direct effect on uxorilocal marriage.