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El presente trabajo de investigación se realizó con el objetivo de elaborar e implementar un plan de mejoras, dirigido a impulsar el desarrollo económico de los emprendedores ubicados en las proximidades del río Tabiazo, Esmeraldas – Ecuador, quienes han manifestado la necesidad de mejorar sus productos e ingresos. La investigación tiene un enfoque mixto de tipo descriptivo, que requiere el trabajo de campo y la revisión bibliográfica para la obtención de información real, y para la puesta en marcha del plan de mejoras. Los resultados obtenidos al término del presente trabajo son de suma importancia puesto que evidencian que los emprendimientos del sector son empíricos, y que los ingresos que perciben podrían incrementar a corto plazo con la puesta en práctica de los conocimientos compartidos. Se llega a la conclusión que para ayudar a las distintas comunidades aisladas del país a mejorar su calidad de vida y los ingresos que perciben, es indispensable que puedan contar con un plan de mejoras dirigido a aprovechar los recursos del sector y a potenciar las actividades y destrezas de los pobladores, respetando sus costumbres y cultura.

The thermoinhibition of lettuce seed germination causes important losses for producers, who do not have thermotolerant commercial cultivars. One of the obstacles has been the scarcity of optimizing techniques capable of efficiently discriminating thermotolerant and thermosensitive cultivars. The aim of this work was to evaluate the use of neural networks to discriminate different levels of thermoinhibition in lettuce seeds. Seeds of 18 cultivars were evaluated for thermoinhibition considering the characteristics of the first and last germination count and germination speed index, in seeds subjected to temperatures of 20, 25, 30 and 35 °C. The remaining seeds, which did not germinate, were subjected to the tetrazolium test. Analyses were performed immediately after seed harvesting and repeated after six months of storage. Discriminant analysis was performed and the Kohonen’s Self-Organizing Map (SOM) was created using Artificial Neural Networks (ANNs). Neural networks discriminate lettuce cultivars and organizes them in terms of seed thermoinhibition tolerance through Kohonen’s Self-Organizing Map. Discriminant analysis consistently identifies the Everglades and Luiza genotypes as tolerant to thermoinhibition. Resumo: A termoinibição causa perdas importantes para os produtores, os quais não dispõem de cultivares comerciais com sementes termotolerantes. Há escassez de técnicas otimizadoras capazes de discriminar cultivares termotolerantes e termosensíveis com eficiência. Objetivou-se avaliar o uso de redes neurais para discriminar diferentes níveis de termoinibição em sementes de alface. Foram avaliadas sementes de 18 cultivares quanto à termoinibição considerando às características de primeira e última contagem de germinação e índice de velocidade de germinação, em sementes submetidas às temperaturas de 20, 25, 30 e 35 °C. As sementes remanescentes, que não germinaram, foram submetidas ao teste de tetrazólio. As análises foram realizadas imediatamente após a colheita das sementes e repetidas após seis meses de armazenamento. Uma análise discriminante e o Mapa Auto-Organizável de Kohonen (SOM) por Redes Neurais Artificiais (RNA’s) foram realizados. As redes neurais discriminam as cultivares de alface e as organiza quanto a tolerância a termoinibição das sementes por meio do Mapa Auto-Organizável de Kohonen. A análise discriminante indentifica de maneira coerente o genótipo Everglades e Luiza como tolerantes a termoinibição.

O presente ensaio tem como objetivo realizar um estudo sobre dois Coletivos Culturais Cineclubistas da Baixada Fluminense, o Mate com Angu e Buraco do Getúlio, traçando um conjunto de panoramas sobre suas potencialidades educativas e de ocupação do espaço urbano através das relações entre os seus participantes, sujeitos periféricos (D’ANDREA, 2013), situados nos grupos que pluralizam e diversificam as juventudes contemporâneas (FEIXA, 2004; MARTÍN-BARBERO, 1997; DAYRELL, 2007). A partir deste estudo, são discutidos os conceitos de coletivo cultural e de cineclube, à luz da bibliografia disponível na plataforma de periódicos da CAPES e de pesquisa em documentos históricos, como revistas e estatutos que apresentam definições da prática cineclubista. Procurando refletir sobre a dualidade entre centro e periferia, a partir da Baixada Fluminense, contexto onde esses Coletivos se mobilizam, discutimos sobre o direito à cultura na cidade e a forma de atuação dos coletivos culturais através de entrevistas realizadas com integrantes dos Coletivos Culturais Cineclubistas. Por fim, apresentamos um conjunto de panoramas, refletindo sobre temas como a cotidianidade, as cidades, as culturas em sua estrita relação à juventude contemporânea, mais especificamente dos sujeitos periféricos.

Precision Agriculture considers soil attributes and production aspects to define management zones. Although there is little information, it is likely that seeds produced in different management zones have different quality after chemical treatment and storage. The aim was to evaluate the physiological quality of sorghum seeds produced in different management zones, after chemical treatment and storage. The management zones were defined from interpolated maps of soil and vegetation attributes with the aid of the Smart Map - SMP plugin, in a Qgis environment. After harvest, the seeds were treated with Thiamethoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxyl-M and a control treatment (water). Then, they were stored for 0, 60 and 120 days. The seeds were evaluated for their quality through the following tests: moisture, germination, dry mass of seedlings, electrical conductivity, and accelerated aging. The design was completely randomized in a 4 × 3 factorial scheme, with the management zones being analyzed independently. Sorghum seed treatments with insecticides cause greater phytotoxicity during storage in both management zones, unlike the fungicide treatment. Sorghum seeds produced in high-management zones have high physiological quality and less deterioration during storage. Sorghum seeds produced in low-management zones treated with insecticides, as the storage time increases, show lower vigor due to deterioration and phytotoxicity. Resumo: A Agricultura de Precisão considera atributos de solo e aspectos produtivos para elaboração de zonas de manejo. Apesar de poucas informações, é provável que sementes produzidas em diferentes zonas de manejo tenham qualidade distinta após o tratamento químico e armazenamento. Objetivou-se avaliar a qualidade fisiológica de sementes de sorgo produzidas em diferentes zonas de manejo, após o tratamento químico e o armazenamento de sementes. As zonas de manejo foram definidas a partir de mapas interpolados de atributos de solo e vegetação com auxílio do plugin Smart Map - SMP, em ambiente Qgis. Após a colheita, as sementes foram tratadas com Thiametoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxil-M e um tratamento controle (água). Em seguida foram armazenadas por 0, 60 e 120 dias. As sementes foram avaliadas quanto a sua qualidade por meio dos testes: de umidade, germinação, massa seca de plântulas, condutividade elétrica e envelhecimento acelerado. O delineamento foi inteiramente casualizado em esquema fatorial 4 × 3, sendo as zonas de manejo analisadas de forma independente. Os tratamentos de sementes de sorgo com inseticidas causam maior fitotoxidez no armazenamento em ambas as zonas de manejo ao contrário do tratamento fungicida. Sementes de sorgo produzidas em zonas de alto manejo possuem alta qualidade fisiológica e com menor deterioração no armazenamento. Sementes de sorgo produzidas em zonas de baixo manejo tratadas com inseticidas mediante aumento dos períodos de armazenamento apresentam menor vigor devido a deterioração e fitotoxidez das sementes.

Precision Agriculture considers soil attributes and production aspects to define management zones. Although there is little information, it is likely that seeds produced in different management zones have different quality after chemical treatment and storage. The aim was to evaluate the physiological quality of sorghum seeds produced in different management zones, after chemical treatment and storage. The management zones were defined from interpolated maps of soil and vegetation attributes with the aid of the Smart Map - SMP plugin, in a Qgis environment. After harvest, the seeds were treated with Thiamethoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxyl-M and a control treatment (water). Then, they were stored for 0, 60 and 120 days. The seeds were evaluated for their quality through the following tests: moisture, germination, dry mass of seedlings, electrical conductivity, and accelerated aging. The design was completely randomized in a 4 × 3 factorial scheme, with the management zones being analyzed independently. Sorghum seed treatments with insecticides cause greater phytotoxicity during storage in both management zones, unlike the fungicide treatment. Sorghum seeds produced in high-management zones have high physiological quality and less deterioration during storage. Sorghum seeds produced in low-management zones treated with insecticides, as the storage time increases, show lower vigor due to deterioration and phytotoxicity.

We reported a case of upper eyelid necrosis initially misdiagnosed as a preseptal cellulitis following a hordeolum externum resulting in great damage to the upper eyelid (anterior lamella). The infection was successfully treated with surgical cleansing, drainage, and endovenous antibiotics. Early treatment may avoid severe complications such as eyelid deformity, systemic involvement, and blindness.

A colheita do café é uma das atividades mais onerosas e impacta diretamente no custo de produção e qualidade, portanto é uma etapa que merece muita ateção. Porém, uma das limitações da colheita, seja no sistema manual ou mecanizado, é a desuniformidade de maturação, que prejudica o desempenho operacional e a qualidade do produto final, gerando perdas econômicas aos produtores. A presente pesquisa foi desenvolvida na Fazenda Santa Rita, município de Machado - MG, e objetivou avaliar o tempo de secagem, a qualidade física e sensorial do café cereja descascado com e sem aplicação de etileno, submetidos a três manejos de secagem. O experimento instalado em esquema fatorial 3 x 2 (3 metodologias de secagem associado a sem e com uso de etileno) em blocos casualizados com 4 repetições para cada tratamento, totalizando 24 unidades experimentais, com 30 litros de café cada. Foram colhidos 5500 litros de café da gleba que foi submetida à aplicação de etileno, e da gleba que não foi submetida, processados até obter a porção cereja descascado dos lotes. Após a secagem, as amostras foram submetidas a um período de descanso de 30 dias, beneficiadas e enviadas para análises de qualidade. Foi analisado o tempo de secagem a qualidade através da prova de xícara pela metodologia SCAA por 3 classificadores de instituições credenciadas. Entre os métodos de secagem orientado terreiro concreto, normal terreio concreto e suspendo orientado recomenda-se o primeiro por proporcionar menor tempo de secagem e não haver diferenças na qualidade do café. A aplicação do etileno não interferiu nas características avaliadas.

O presente artigo apresenta algumas das ações de um programa de extensão da UFRJ no período da pandemia de Covid-19. Durante o funcionamento regular das instituições de ensino, o grupo concentra seus esforços em promover aulas de iniciação ao cinema em escolas e hospitais públicos da cidade do Rio de Janeiro. Nesse sentido, foi necessário adaptar a proposta extensionista, respeitando as regras impostas no ano atípico de 2020, inventando e reinventando modos de viabilizar a continuidade das ações, de forma a manter o previsto na Resolução Nº 07, de 18 de dezembro de 2018 (que prevê a indissociabilidade do tripé universitário – pesquisa, ensino, extensão – e afirma a obrigatoriedade de cumprimento de pelo menos 10% do total da carga horária dos cursos de graduação em extensão). Durante a pandemia, diversas ações foram realizadas, entre estas um Curso de Extensão para vinte e cinco estudantes de graduação, além de encontros semanais com leituras de textos, visando a produção de materiais e a divulgação científica através das redes sociais do programa. Ainda durante esse período, o programa tem realizado minicursos e divulgado materiais em eventos acadêmicos universitários, tanto na UERJ quanto na UFRJ, além de organizar uma mostra virtual na Cinemateca do Museu de Arte Moderna do Rio.

Western blotting is a standard laboratory method used to detect proteins and assess their expression levels. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader’s ability to evaluate or reproduce western blot results. While several groups have studied the prevalence of image manipulation or provided recommendations for improving western blotting, data on the prevalence of common publication practices are scarce. We systematically examined 551 articles published in the top 25% of journals in neurosciences (n=151) and cell biology (n=400) that contained western blot images, focusing on practices that may omit important information. Our data show that most published western blots are cropped and blot source data are not made available to readers in the supplement. Publishing blots with visible molecular weight markers is rare, and many blots additionally lack molecular weight labels. Western blot methods sections often lack information on the amount of protein loaded on the gel, blocking steps and antibody labeling protocol. Important antibody identifiers like source, catalog number or RRID were omitted frequently for primary antibodies, and regularly for secondary antibodies. We present detailed descriptions and visual examples to help scientists, peer reviewers and editors to publish more informative western blot figures and methods. Additional resources include a toolbox to help scientists produce more reproducible western blot data, teaching slides in English and Spanish and an antibody reporting template.

The recent rise of right-wing populism has given new momentum to the discussion about the meaning of populism around the world and the covid-19 pandemic added yet another layer to the issue. Considering the growing circulation of the term “populism” in public discourse, as well as the volatility of its meaning, this paper seeks to identify the different constructions of meaning around this phenomenon by Brazilian media during the covid-19 pandemic. Through quantitative and qualitative analysis, in which we used the perspective of media framings, we examine 170 articles from 17 Brazilian media outlets published between March 1st and October 1st, 2020. The results allow us to conclude that there are circumstantial meanings of populism that were incorporated during the pandemic, especially related to an antiscientific approach. RESUMO A recente ascensão do populismo de direita tem dado um novo impulso à discussão sobre o significado do populismo em todo o mundo e a pandemia da covid-19 acrescentou uma camada complementar à questão. Considerando a crescente circulação do termo “populismo” no discurso público, bem como a volatilidade de seu significado, este artigo busca identificar as diferentes construções de sentido em torno deste fenômeno nos meios de comunicação brasileiros durante a pandemia da covid-19. Por meio de análises quantitativas e qualitativas, nas quais mobilizamos a perspectiva dos enquadramentos midiáticos, examinamos 170 artigos de 17 veículos de comunicação brasileiros publicados entre 1º de março e 1º de outubro de 2020. Os resultados permitem concluir que há sentidos circunstanciais do populismo que são incorporados durante a pandemia, especialmente relacionados a uma abordagem anticientífica. RESUMEN El reciente aumento del populismo de derecha ha dado un nuevo impulso a la discusión sobre el significado del populismo en todo el mundo y la pandemia de covid-19 agregó una capa más al problema. Considerando la creciente circulación del término “populismo” en el discurso público, así como la volatilidad de su significado, este trabajo busca identificar las diferentes construcciones de significado en torno al fenómeno por los medios brasileños durante la pandemia de covid-19. A través de un análisis cuantitativo y cualitativo, en el que utilizamos la perspectiva de los encuadres mediáticos, examinamos 170 artículos de 17 medios brasileños publicados entre el 1 de marzo y el 1 de octubre de 2020. Los resultados brindan elementos que nos permiten concluir que durante la pandemia se incorporaron significados circunstanciales del populismo, especialmente relacionados con un enfoque anticientífico.

Made available in DSpace on 2022-04-29T08:46:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Background: Patients hospitalised with COVID-19 are at risk for thrombotic events after discharge; the role of extended thromboprophylaxis in this population is unknown. Methods: In this open-label, multicentre, randomised trial conducted at 14 centres in Brazil, patients hospitalised with COVID-19 at increased risk for venous thromboembolism (International Medical Prevention Registry on Venous Thromboembolism [IMPROVE] venous thromboembolism [VTE] score of ≥4 or 2–3 with a D-dimer >500 ng/mL) were randomly assigned (1:1) to receive, at hospital discharge, rivaroxaban 10 mg/day or no anticoagulation for 35 days. The primary efficacy outcome in an intention-to-treat analysis was a composite of symptomatic or fatal venous thromboembolism, asymptomatic venous thromboembolism on bilateral lower-limb venous ultrasound and CT pulmonary angiogram, symptomatic arterial thromboembolism, and cardiovascular death at day 35. Adjudication was blinded. The primary safety outcome was major bleeding. The primary and safety analyses were carried out in the intention-to-treat population. This trial is registered at ClinicalTrials.gov, NCT04662684. Findings: From Oct 8, 2020, to June 29, 2021, 997 patients were screened. Of these patients, 677 did not meet eligibility criteria; the remaining 320 patients were enrolled and randomly assigned to receive rivaroxaban (n=160 [50%]) or no anticoagulation (n=160 [50%]). All patients received thromboprophylaxis with standard doses of heparin during hospitalisation. 165 (52%) patients were in the intensive care unit while hospitalised. 197 (62%) patients had an IMPROVE score of 2–3 and elevated D-dimer levels and 121 (38%) had a score of 4 or more. Two patients (one in each group) were lost to follow-up due to withdrawal of consent and not included in the intention-to-treat primary analysis. The primary efficacy outcome occurred in five (3%) of 159 patients assigned to rivaroxaban and 15 (9%) of 159 patients assigned to no anticoagulation (relative risk 0·33, 95% CI 0·12–0·90; p=0·0293). No major bleeding occurred in either study group. Allergic reactions occurred in two (1%) patients in the rivaroxaban group. Interpretation: In patients at high risk discharged after hospitalisation due to COVID-19, thromboprophylaxis with rivaroxaban 10 mg/day for 35 days improved clinical outcomes compared with no extended thromboprophylaxis. Funding: Bayer. Science Valley Research Institute Hospital e Maternidade Christóvão da Gama Grupo Leforte Santo André Unidade de Medicina Interdisciplinar em Cardiologia Instituto do Coração Hospital das Clínicas HCFMUSP Faculdade de Medicina Universidade de São Paulo Zucker School of Medicine at Hofstra/Northwell and the Feinstein Institutes for Medical Research Department of Obstetrics and Gynecology I M Sechenov First Moscow State Medical University Universidade Estadual Paulista Hospital das Clínicas de Ribeirão Preto São Paulo University Medical School Ribeirão Preto Hospital do Rocio, Campo Largo Instituto Couto Maia Salvador Hospital Municipal de Barueri São Paulo State Public Women's Health Reference Center Hospital Israelita Albert Einstein Instituto do Coração Hospital das Clínicas HCFMUSP Faculdade de Medicina Universidade de São Paulo Institute of Teaching and Research Hapvida Hospital Nossa Senhora das Graças Department of Statistics Institute of Mathematics and Statistics University of São Paulo Northshore University Health System Hemostasis and Thrombosis Research Laboratories at Loyola University Medical Center Duke Clinical Research Institute Duke University School of Medicine Universidade Estadual Paulista

Made available in DSpace on 2022-04-29T08:46:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 BACKGROUND: The devastating Coronavirus disease (COVID-19) pandemic is associated with a high prothrombotic state. It is unclear if the coagulation abnormalities occur because of the direct effect of SARS-CoV-2 or indirectly by the cytokine storm and endothelial damage or by a combination of mechanisms. There is a clear indication of in-hospital pharmacological thromboprophylaxis for every patient with COVID-19 after bleed risk assessment. However, there is much debate regarding the best dosage regimen, and there is no consensus on the role of extended thromboprophylaxis. DESIGN: This study aims to evaluate the safety and efficacy of rivaroxaban 10 mg once daily for 35 ± 4 days versus no intervention after hospital discharge in COVID-19 patients who were at increased risk for VTE and have received standard parenteral VTE prophylaxis during hospitalization. The composite efficacy endpoint is a combination of symptomatic VTE, VTE-related death, VTE detected by bilateral lower limbs venous duplex scan and computed tomography pulmonary angiogram on day 35 ± 4 posthospital discharge and symptomatic arterial thromboembolism (myocardial infarction, nonhemorrhagic stroke, major adverse limb events, and cardiovascular death) up to day 35 ± 4 posthospital discharge. The key safety outcome is the incidence of major bleeding according to ISTH criteria. SUMMARY: The MICHELLE trial is expected to provide high-quality evidence around the role of extended thromboprophylaxis in COVID-19 and will help guide medical decisions in clinical practice.1. Science Valley Research Institute, Santo André, São Paulo, Brazil; Hospital e Maternidade Christóvão da Gama, Grupo Leforte, Santo André, São Paulo, Brazil Science Valley Research Institute Interdisciplinary Medicine in Cardiology Unit Heart Institute (InCor)- University of São Paulo Medical School Universidade Estadual Paulista (UNESP) Hospital das Clínicas de Ribeirão Preto São Paulo University Medical School (USP) Irmandade da Santa Casa da Misericórdia de Santos Hospital Ana Neri Hospital Municipal de Barueri São Paulo State Public Women's Health Reference Center Hospital Israelita Albert Einstein Heart Institute (InCor)- University of São Paulo Medical School Institute of Teaching and Research Hapvida CE Hospital Nossa Senhora das Graças Department of Statistics Institute of Mathematics and Statistics University of Sao Paulo Northshore University Health System Zucker School of Medicine at Hofstra/Northwell and the Feinstein Institutes for Medical Research, Manhasset, NY Duke Clinical Research Institute Duke University School of Medicine Universidade Estadual Paulista (UNESP)

International audience; Je suis partie d'une première publication, celle du colloque organisé à l'IHEAL sur « Filiation, polyphonie et théâtralité en Amérique latine », dont les communications ont été diffusées sur le site de l'ADAL, et certaines dans la revue Mots n° 93, 2010 : Figures et filiation dans le discours politique latino-américain. Ce numéro était marqué par la montée au pouvoir de présidents « de gauche » en Amérique latine, présidents qui s'inscrivaient dans les dires de « figures politiques tutélaires » : Chávez citant Bolívar au Venezuela, mais avant lui Castro citant Martí, qui n'était pas marxiste, mais qui renaît en héros fondateur d'un éthos révolutionnaire, que Castro réinsère dans « l'imaginaire politique ». C'est donc tout naturellement que les premiers colloques internationaux organisés par l'ADAL portent sur les ”Représentations et imaginaires dans les discours politiques en Amérique latine” et sur « Les leaders et les leaderships présidentiels » . Depuis dix ans, dans les travaux de l'ADAL, des concepts et des notions sont ainsi convoqués, et « re-travaillés » par les auteurs à des fins d'analyse des discours politiques et institutionnels, participant ainsi à une meilleure compréhension de l'histoire politique des pays latino-américains. On y trouve des analyses minutieuses, certaines « outillées », de mots du discours et de leurs cotextes et contextes, proches des travaux actuels d'une sémantique de discours pratiquée en sciences du langage (Moirand 2019a), mais on resterait aux frontières du discours politique si on ne croisait pas ces résultats aux concepts englobants venus de la philosophie ou de la sociologie politiques, et bien sûr à l'Histoire. La visée, à la fois rétrospective et prospective, des travaux de l'ADAL permet de s'interroger aujourd'hui sur le fonctionnement et le devenir des démocraties représentatives, voire des démocraties « participatives » en Amérique latine, ainsi que sur les formes nouvelles de surgissement de certains acteurs dans la scène politique. C'est donc un voyage à travers les notions rencontrées dans les colloques de l'ADAL depuis dix ans que l'on propose dans une première partie, qui se prolonge en deuxième partie par une réflexion sur l'occupation de l'espace par de nouveaux acteurs jusque-là peu visibles, et ses conséquences pour l'étude des discours de/sur l'Amérique latine.

This paper presents the development of a new methodology for evaluation and distribution of patent applications to the examiners at the Brazilian Patent Office considering a specific technological field, represented by classification of the application according to the International Patent Classification (IPC), and the variables corresponding to the volume of data of the application and its complexity for the examination process. After identifying the most relevant variables, such as the Specific Areas of Expertise (ZAE) of the examiners, a mathematical model was developed, including: (a) application of the principal component analysis (PCA) method; (b) calculation of a General Complexity Ratio (IGC); (c) classification into five classes (very light, light, moderate, heavy and very heavy) according to IGC average ranges and standard deviations; (d) implementation of a logic of distribution, compensating very heavy applications with very light ones, and light applications with heavy ones; and (e) calculation of a Distribution Balancing Ratio (IBD), considering the differences between the samples’ medians. The model was validated using a sample of patent applications including, in addition to the identified variables, the time for substantive examination by the examiner. Then, a correlation analysis of the variables with time and a comparison of the classifications according to the time and the IGC generated by the model were carried out. The results obtained showed a high correlation of the IGC with time, above 80%, as well as correct IGC classes in more than 80% of applications. The model proposed herein suggests that the three main relevant variables are: total number of pages, total number of claims, and total number of claim pages.

Removal of lettuce basal leaves is a technique used by the seed industry; however, the effects on seed production and physiological potential are unknown. Genetic dissimilarity is fundamental in the identification of individuals in terms of traits of interest, although it is rarely considered in relation to seeds that are tolerant to thermoinhibition. The aim of this study was to ascertain the relationship between defoliation of lettuce plants and seed yield/physiological quality, as well as the genetic dissimilarity among genotypes with regard to seed thermoinhibition. We used 35 lines of biofortified lettuce, the cultivars Uberlândia 10000, Belíssima, UFU MC BIOFORT1, and Everglades (tolerant to thermoinhibition), and Grand Rapids and Verônica (susceptible to thermoinhibition). The seed yield and physiological quality of the genotypes with and without defoliation were evaluated, artificially aged, and germinated at four temperatures in a factorial arrangement. The genetic dissimilarity was estimated by Tocher graphing and the UPGMA clustering method, based on the Mahalanobis generalized distance (D2ii). Four UFU genotypes and Grand Rapids had high seed yields. With removal of basal leaves, there was higher seed yield per plant and there was an effect on their physiological quality. The genotypes exhibited genetic variability for thermoinhibition, with UFU-86#2#1#1 and Everglades showing similar performance. Removal of lettuce basal leaves is a technique used by the seed industry; however, the effects on seed production and physiological potential are unknown. Genetic dissimilarity is fundamental in the identification of individuals in terms of traits of interest, although it is rarely considered in relation to seeds that are tolerant to thermoinhibition. The aim of this study was to ascertain the relationship between defoliation of lettuce plants and seed yield/physiological quality, as well as the genetic dissimilarity among genotypes with regard to seed thermoinhibition. We used 35 lines of biofortified lettuce, the cultivars Uberlândia 10000, Belíssima, UFU MC BIOFORT1, and Everglades (tolerant to thermoinhibition), and Grand Rapids and Verônica (susceptible to thermoinhibition). The seed yield and physiological quality of the genotypes with and without defoliation were evaluated, artificially aged, and germinated at four temperatures in a factorial arrangement. The genetic dissimilarity was estimated by Tocher graphing and the UPGMA clustering method, based on the Mahalanobis generalized distance (D2ii). Four UFU genotypes and Grand Rapids had high seed yields. With removal of basal leaves, there was higher seed yield per plant and there was an effect on their physiological quality. The genotypes exhibited genetic variability for thermoinhibition, with UFU-86#2#1#1 and Everglades showing similar performance.

Western blotting is a standard laboratory method used to detect proteins and assess their expression levels. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader’s ability to evaluate or reproduce western blot results. While several groups have studied the prevalence of image manipulation or provided recommendations for improving western blotting, data on the prevalence of common publication practices are scarce. We systematically examined 551 articles published in the top 25% of journals in neurosciences (n = 151) and cell biology (n = 400) that contained western blot images, focusing on practices that may omit important information. Our data show that most published western blots are cropped and blot source data are not made available to readers in the supplement. Publishing blots with visible molecular weight markers is rare, and many blots additionally lack molecular weight labels. Western blot methods sections often lack information on the amount of protein loaded on the gel, blocking steps, and antibody labeling protocol. Important antibody identifiers like company or supplier, catalog number, or RRID were omitted frequently for primary antibodies and regularly for secondary antibodies. We present detailed descriptions and visual examples to help scientists, peer reviewers, and editors to publish more informative western blot figures and methods. Additional resources include a toolbox to help scientists produce more reproducible western blot data, teaching slides in English and Spanish, and an antibody reporting template.

The objective of this work was to investigate the efficiency of the accelerated aging and controlled deterioration tests in the evaluation of lentil seeds’ vigor. Water content was determined and the physiological quality was evaluated using germination and vigor tests (first count, seedling emergence, indices of germination speed and emergence, accelerated aging test with traditional method and with NaCl saturated solution at 41oC and 45oC for 24, 48, 72 hours and controlled deterioration with 20% and 24% water content for 24, 48 and 72 hours at 45oC) in seven lots of lentil seeds cv Silvina. The use of traditional accelerated aging test and the NaCl saturated solution method at 41oC for 48 hours were efficient to classify lentil seeds’ lots. The combination of 20% and 24% water for 48 hours exposure and of 20% water for 72 hours exposure were efficient to classify the lentil seed lots by the controlled deterioration test.

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi. As sementes de maxixe geralmente apresentam qualidade fisiológica variável. Empresas produtoras de sementes de hortaliças necessitam de testes rápidos e confiáveis para a avaliação da qualidade das sementes. O teste de germinação é o método oficial de análise, porém a tecnologia de sementes tem procurado aprimorar testes de vigor para a avaliação do potencial fisiológico da semente. Nesse sentido, objetivou-se avaliar procedimentos para a condução do teste de envelhecimento acelerado e determinar o potencial fisiológico e sanitário de sementes de maxixe. Para isso, utilizaram-se quatro lotes de sementes da cultivar Liso Calcutá. Para a avaliação da qualidade fisiológica inicial determinou-se o teor de água e utilizou-se os testes de germinação, emergência e índices de velocidade de germinação e emergência. O teste de envelhecimento acelerado foi conduzido pelos procedimentos tradicional e com solução salina saturada, nos períodos de 48, 72 e 96 horas, nas temperaturas de 41 oC e 45 oC. Após o envelhecimento, determinou-se o teor de água e as sementes foram submetidas ao teste de germinação e sanidade. O delineamento foi inteiramente casualizado em esquema fatorial 4x3x2 (lotes x períodos de envelhecimento x temperaturas). O teste de envelhecimento acelerado conduzido no método tradicional e com solução salina saturada a 41 ºC por 96 horas é eficiente para avaliar o vigor de sementes de maxixe. A solução salina saturada proporciona absorção de água e deterioração uniforme das sementes de maxixe permitindo a diferenciação dos lotes de sementes em diferentes níveis de vigor. O teste de sanidade após o envelhecimento acelerado com solução salina, proporciona diminuição da incidência de alguns fungos.

Croisant les apports des sciences de l’information et de la communication, de la sociologie professionnelle et de la science politique, cette thèse interroge les enjeux démocratiques de la professionnalisation de la communication politique gouvernementale en France et au Brésil. Indissociable de l’activité politique et fondement des sociétés démocratiques, la communication est une composante des régimes représentatifs. Néanmoins, les transformations de la visibilité publique des acteurs et institutions politiques et des manières de communiquer des gouvernements posent la question sur les effets de son développement comme spécialité dans le champ politique sur le fonctionnement des systèmes démocratiques contemporains. À l’appui d’une enquête menée auprès de 22 conseillers en communication ayant travaillé pour des Présidents et Ministres français et brésiliens entre 1995 et 2019 et 1985 et 2017, respectivement, ce travail vise ainsi à identifier, à caractériser et à analyser les modèles de professionnalisation de la communication politique gouvernementale dans ces deux pays. De surcroît, nous mettons en exergue les rapports entre les processus de professionnalisation s’opérant dans ces espaces particuliers de la vie collective et le sentiment de complexification de la politique dans les démocraties contemporaines. En privilégiant la perspective comparative et l’approche compréhensive des discours des responsables de la communication politique au gouvernement en France et au Brésil, cette étude met l’accent sur le rôle, les motivations et les conceptions de communication orientant le travail de ces acteurs dans les deux pays. Ce travail montre que, en dépit des différences structurant ce milieu en France et au Brésil, la constitution de ce groupe d’acteurs présente un caractère cloisonné, peu propice au renouvellement et soumis à des impératifs politiques et économiques. Combinando as contribuições das ciências da informação e da comunicação, da sociologia profissional e da ciência política, esta tese questiona as implicações para a democracia da profissionalização da comunicação política governamental na França e no Brasil. Inseparável da atividade política e fundamento das sociedades democráticas, a comunicação é uma constitutiva dos regimes representativos. No entanto, as transformações da visibilidade pública dos atores e instituições políticas e das formas de comunicar dos governos jogam luz sobre os efeitos do seu desenvolvimento como especialidade dentro do campo político para o funcionamento dos sistemas democráticos contemporâneos. Com base em uma pesquisa realizada com 22 assessores de comunicação que trabalharam para presidentes e ministros franceses e brasileiros de 1995 a 2019 e de 1985 a 2017, respectivamente, este trabalho objetiva identificar, caracterizar e analisar os modelos de profissionalização da comunicação política do governo nesses dois países. Além disso, destacamos as relações entre os processos de profissionalização que ocorrem nesses espaços particulares da vida coletiva e o sentimento de complexificação da política nas democracias contemporâneas. Ao privilegiar a perspectiva comparativa e a abordagem compreensiva dos discursos dos responsáveis pela comunicação política do governo na França e no Brasil, este estudo enfatiza o papel, as motivações e as concepções de comunicação que norteiam o trabalho desses atores em ambos os países. Este trabalho mostra que, apesar das diferenças que estruturam esse meio na França e no Brasil, a constituição desse grupo de atores apresenta um caráter hermético, pouco favorável à renovação e submetido a imperativos políticos e econômicos. By crossing the contributions of information and communication sciences, professional sociology and political science, this thesis questions the democratic challenges of the professionalization of political government communication in France and Brazil. As an integral part of the political activity and as the foundation of democratic societies, the spread of communication as a specialty linked to politics leads to consider it as a component of the transformations of contemporary representative regimes. However, the reconfiguration of the public visibility of political actors and institutions, as well as the ways of communicating with governments raise the question of the effects of such transformations on the functioning of democratic systems. Using the interviews of 22 communication advisers who worked for French and Brazilian Presidents and Ministers respectively between 1995 and 2019 and 1985 and 2017, this work aims to identify, characterize and analyze the models of professionalization of political government communication in these two countries. In addition, we highlight the relationships between the professionalization processes taking place in these particular areas of collective life and the feeling of complexification of politics in contemporary democracies. With a special emphasis on the comparative perspective and the comprehensive approach of the speeches of those responsible for political communication to the government in France and Brazil, this study emphasizes the role, motivations and conceptions of communication guiding the work of these actors in the two countries. This work shows that, in spite of the differences structuring this environment in France and in Brazil, the constitution of this group of actors shows a compartmentalization. While this effect is subjected to political and economic imperatives, it is also shown to hinder a certain form of renewal.

Brazil is a privileged country in relation to their mineral resources, but have problems in diagnosis the situation about the surface water and groundwater, because the quality and the quantity of water. Questions about physico-chemical parameters as high as salinity or low hydraulic conductivity can affect the use of this underground water resource. The study of procedures for diagnostic directly or indirectly in salinity in groundwater can assist the selection of sites for acquisition. In this direction, this study describes a comparative analysis between geochemical analyzes and tests on geophysical study of saline free aquifer, in an attempt to establish correlations between data, whether direct or indirect, in addition to providing an analysis of the limitations of the DC Resistivity geophysical method as indicator tool of degrees of salinity in an indirect way. The geological site consist in clay soils settled on siltstone and argillites of the Corumbataí Formation, of the Basin of Paraná Sedimentary Basin in Brazil. This unit represents an aquiclude regional, the occurrence of level free aquifer in the contact soil/rock characterized by variable salinity and is widely used as a source of supply for rural properties. The physical-chemical parameters measured in samples collected in wells were: total dissolved solids (TDS), electrical conductivity (CE), in addition to measures of hydraulic conductivity (k). The data of TDS and CE were widely variable, but resulted in a direct correlation. The comparison between the data from electrical resistivity with TDS and electrical resistivity with hydraulic conductivity do not result in satisfactory correlations perchance the complexity geological and a high variable in salinity. Factors that control electrical resistivity in the geological environment.

En esta publicación se presentan los resultados obtenidos de la implementación de una infraestructura para mejorar los servicios tecnológicos de correo electrónico, entorno virtual de aprendizaje, repositorio digital y biblioteca virtual en la Escuela Superior Politécnica Agropecuaria de Manabí, ESPAM, mediante la utilización de mecanismos de alta disponibilidad y virtualización para ofrecer recursos de mayor confiablidad. La virtualización es una tecnología potenciadora de vanguardia que está transformado el funcionamiento de servicios tecnológicos, pero, implica un cambio de paradigma en las tecnologías de la información orientada a los servicios y la computación en la nube. Para ejecutar cada uno de los procesos, se empleó, como estrategia, la metodología ciclo en V. Los servicios de virtualización fomentan que las empresas e instituciones transformen su forma de operar para estar a la vanguardia en innovación de sus servicios como solución tecnológica, es así que la implementación de tecnología redundante en la ESPAM, le ha permitido que sus servicios tecnológicos estén siempre operativos, en beneficio de la comunidad universitaria, ya que si se presentaren fallos en el sistema principal o los servicios, se habilitarán rápidamente los respaldos permitiendo que los sistemas entren en operación de inmediato.

Os testes de vigor foram desenvolvidos para compensar as deficiências do teste de germinação. Objetivou-se avaliar a utilização do teste de condutividade elétrica na avaliação do potencial fisiológico de sementes de grão de bico. Quatro lotes de sementes da cultivar BRS Aleppo, foram submetidos a testes de germinação e vigor (primeira contagem da germinação, emergência das plântulas, índice de velocidade de emergência e envelhecimento acelerado). Para o teste de condutividade elétrica, foram estudadas variações no volume de água (50 e 75mL), temperatura (25 e 30ºC), número de sementes (25 e 50) e tempo de embebição (1, 2, 4, 6, 12, 18 e 24 horas). O teste de condutividade elétrica foi eficiente para a avaliação do vigor de sementes de grão de bico, com o tempo de embebição podendo ser reduzido para quatro horas, porém a utilização de 50 sementes não permitiu diferenciar os lotes. O volume de 25mL em temperatura de 30°C foi a condição mais favorável para a ordenação consistente dos lotes quanto ao vigor, de forma que a condição mais adequada para o teste de condutividade elétrica em sementes de grão de bico é a utilização de 25 sementes em 50mL de água por quatro horas a 30oC., Revista de Ciências Agrárias, Vol 42 No 3 (2019)

The search for vigor tests that offer fast and reliable information about the physiological potential of seeds is fundamental in quality control. The present study aimed to evaluate the effect of different temperatures associated with the reduction of soybean imbibition period in the electrical conductivity test. Seven seed lots of the cultivar TMG 7062 IPRO were used in this assessment. For the initial characterization of the lots, we determined the water content, the first and the final count of the germination test, the emergence, the accelerated aging, and the traditional electrical conductivity. The other method used for the electrical conductivity test consisted of 20 replicates of 10 seeds which were placed in plastic cups with 25ml of deionized water, and soaked in 10 min, 30 min, 60 min, and 120 min at temperatures of 30oC, 35oC, 40oC, and 45oC. The experimental design was completely randomized. We were able to reduce the imbibition period since its results were similar to those of the traditional methodology. The electrical conductivity test performed using the 30 minutes imbibition period at temperatures of 30oC or 35oC provides consistent information that allows the identification of lots of soybean seeds with different levels of vigor., Revista de Ciências Agrárias, vol. 42 n.º 2 (2019)

A cultura da mandioca e a sua cadeia produtiva necessita de grande aporte de recursos, direcionados a verificar variedades produtivas, com raízes de boa qualidade e que atendam a demanda do processamento mínimo. Objetivou-se com este trabalho avaliar os aspectos agronômicos, a qualidade de raízes e o processamento mínimo de variedades de mandioca de mesa. Foram cultivadas seis variedades de mandioca (Amarelinha, Amarela, Cacau, Sabará, Vassourinha e Assentamento Estrela do Norte) em delineamento de blocos ao acaso, com quatro repetições. Na colheita aos a10 meses, foram avaliadas a produtividade, índice de colheita, número de raízes por planta, peso médio de raízes por planta e a matéria seca das raízes. Em seguida, o material foi minimamente processado e conservado a 5° ± 2°C e 90 ± 5% de umidade relativa por 15 dias. Foram avaliados: o rendimento agroindustrial, teor de umidade, sólidos solúveis, pH e tempo de cozimento. O delineamento foi inteiramente casualizado, em esquema fatorial 6 x 6, referentes as variedades de mandioca e períodos de armazenamento (0, 3, 6, 9, 12 e 15 dias), com 4 repetições. O rendimento agroindustrial, foi calculado apenas ao final do processamento mínimo. A variedade Vassourinha possui alta produtividade (79,24 t ha-1), peso médio de 4,0 Kg e elevada porcentagem de matéria seca de raízes, com rendimento agroindustrial acima de 70%. Mantém também a qualidade das raízes ao longo de 15 dias de armazenamento, sendo, portanto, sugerida ao processamento mínimo. O tempo de cocção desta variedade de mandioca é de 9 minutos. The cassava crop and its production chain require a large investment of resources, directed to check productive varieties with good roots and that meet the minimum processing chain demand. Aimed with this work to evaluate the agronomic aspects, the quality of roots and minimal processing of cassava varieties. Six cassava varieties were grown (Amarelinha, Amarela, Cacau, Sabará, Vassourinha e Assentamento Estrela do Norte) in design of a randomized block design with four replications. At harvest the a10 months, were assessedproductivity, harvest index, number of roots per plant, average weight of roots per plant and root dry matter. Then, the material was minimally processed and stored at 5 ± 2 ° C and 90 ± 5% relative humidity for15 days. They were evaluated: performance in industrial processing, moisture content, soluble solids, pH and cooking time. The design was completely randomized in a factorial 6 x 6, regarding the varieties of cassava and storage periods (0, 3, 6, 9, 12 and 15 days), with 4 repetitions. The performance in industrial processing was calculated only at the end of the minimum processing. The Vassourinha variety has high yield (79.24 t ha-1), average weight of 4.0 kg and high percentage of dry matter of roots, with performance in industrial processing above 70%. also maintains the quality of the roots over 15 days of storage, and therefore suggested to minimal processing. The cooking time of this variety of cassava is 9 minutes.

Este trabalho trata de um estudo teórico sobre o MMPI. Inicialmente, apre-sentamos um levantamento bibliográfico sobre o MMPI evidenciando os seus aspectos construtivos, seu funcionamento, suas características operacionais e suas possíveis aplicações. Em seguida, elaboramos um modelo físico fundamentado nas teorias do Motor de Indução Duplamente Alimentado (MIDA) e do Motor de Histerese (MH), o qual agrega as principais características operacionais do MMPI e reproduz o processo dinâmico de inscrição de pólos na periferia do rotor. A partir deste modelo físico propomos um modelo matemático, baseado na teoria dos circuitos magneticamente acoplados, constituído pelo equacionamento do MIDA, do MH e das fontes de alimentação do estator e do rotor. Deduzimos teoricamente todos os parâmetros necessários para a simulação computacional. Executamos a sua simulação computacional no software Matlab/SimulinkTM, cujos resultados ilustram o comportamento dinâmico das suas tensões, correntes, fluxos e mecânica. Comparando-se os resultados obtidos na simulação computacional com testes laboratoriais executados pelo CE-PEL, concluímos que o modelo matemático proposto e os parâmetros deduzidos podem ser considerados validados. Finalizando, sugerimos um motor monofásico de pólos lisos a imãs permanentes como uma alternativa mais simples do ponto de vista construtivo, porém com características operacionais semelhantes às do MMPI. This work deals with a theoretical study on Written-PoleTM single-phase motor. Initially, we present the results from the bibliographical research on the Written-PoleTM motor, evidencing its constructional features, operation process, operational characteristics and some possible applications. Afterwards, we elaborate a physical model based in the theories of the doubly fed induction motor and the hysteresis motor, which incorporates the operational characteristic of Written-PoleTM motor and reproduces the written-pole dynamic process. Starting from this physical model we propose a mathematical model, based on the theory of the magnetically coupled circuits, constituted by the set of differential equations deduced from the doubly fed induction motor, the hysteresis motor and the stator and rotor voltage sources. We deduced theoretically all the computational simulation necessary parameters. We perform its computational simulation in the software Matlab/SimulinkTM, whose results illustrate the dynamic behavior of its voltages, currents, fluxes, torques and speed. Comparing the results obtained from the computational simulation with the CEPEL\'s test results, we concluded that the proposed mathematical model and the deduced parameters can be considered validated. Finally, we suggest a permanent-magnet smooth-pole single-phase motor as an alternative to Written-PoleTM motor. This alternative, even being simpler from the constructional point-of-view, presents operational characteristics similar to the Written-PoleTM single-phase motor.

How likely is an invention to turn into an innovation in the short, medium, and long term? Do patent examiners have the power to influence the direction technology takes in a given sector or industry? This study discusses possible impacts arising from the interpretation of the concept of inventive step on the potential for innovation in the electricity area. It also aims to identify any indications of subjectivity in the concept of “inventive step,” of the examiners’ decision-making power, and the influence of the examiners’ evaluations on the direction of technology in the light of the concepts of conformity of action by agents to institutional rules and room for deviation as proposed by Dequech (2013). A case study is presented, comparing the content of first instance examinations and appeal examinations of patent claims in the area of electricity from 2015 to 2017, showing the levels of divergence in the appraisals of inventive step and recommendations for initial decisions to be reversed.

In reducing the cost of production, one alternative is the use of asexual propagation when obtaining tomato seedlings. Use of the appropriate substrate can also result in better rooting and development of the cuttings. The aim of this experiment therefore, was to evaluate the effect of different substrates and means of propagation in the production of tomato seedlings. The experiment was carried out in a greenhouse with cuttings collected from the side shoots of healthy mother plants, sorted into two sizes and hybrid tomato seeds. The seeds and cuttings were grown in two commercial substrates. The experimental design was completely randomised in a 3x2 factorial scheme (propagation x substrate), with four replications, giving a total of 24 lots. There was interaction between the means of propagation and types of substrate for the following characteristics: root fresh weight, shoot fresh weight, length of main root, final length of shoots, root dry weight, shoot dry weight, total fresh weight and total dry weight. Rooting was affected by the substrate, and main root length by the means of propagation. The cuttings of 11 cm in length stood out for making the vegetative propagation of the tomato viable. Using coconut fibre as a substrate promoted better root development = Uma alternativa para diminuir o custo de produção do tomateiro é o uso de propagação assexuada para a obtenção de mudas, além disso, a utilização de um substrato adequado pode promover um melhor enraizamento e desenvolvimento das estacas. Assim, objetivou-se com este trabalho avaliar o efeito de diferentes formas de propagação e substratos na produção de mudas de tomateiro. Realizou-se o experimento em casa de vegetação com estacas coletadas a partir de brotos laterais de plantas matrizes sadias, padronizadas em dois tamanhos e sementes híbridas de tomateiro. As estacas e sementes foram cultivadas em dois substratos comerciais, em delineamento experimental inteiramente casualizado, em esquema fatorial 3x2 (propagação x substratos), quatro repetições, totalizando 24 parcelas. Houve interação entre as formas de propagação e os tipos de substratos para as seguintes características: massa fresca das raízes, massa fresca da parte aérea, comprimento da raiz principal, comprimento final da parte aérea, massa seca da raiz, massa seca da parte aérea, massa fresca total e massa seca total. O enraizamento foi afetado pelo substrato, enquanto o comprimento da raiz principal pelas formas de propagação. As estacas de 11 cm de comprimento se destacaram, tornando a propagação vegetativa do tomateiro viável. A utilização do substrato de fibra de coco promoveu melhor desenvolvimento radicular

Resumo: Este artigo propõe-se a analisar a construção de personagens de ficção biográfica em torno da figura pública do escritor João Guimarães Rosa, com base na confrontação de três matérias jornalísticas versando sobre a morte enigmática do romancista, ocorrida em 19 de novembro de 1967. Cabe ressaltar que, em 2017, celebram-se 50 anos desse evento marcante da cultura brasileira, ainda por se decifrar em todo seu alcance e significação. Por esse viés, o presente estudo parte igualmente do fato de que Grande Sertão: Veredas é qualificado, por seu próprio autor, como uma “autobiografia irracional”: trata-se, de maneira inédita na história da literatura, de contar uma vida (e uma morte), para em seguida vivê-la. Por esse viés, a análise centra-se na dimensão narrativa do texto jornalístico, no plano das imbricações entre universo fático e universo ficcional (MOTTA, 2005). Com esteio em um corpus constituído por três artigos publicados no O Globo, O Estado de São Paulo e Folha de São Paulo, discorre-se sobre os índices semântico-lexicais e as condicionantes editoriais (CHARAUDEAU, 2010) que induzem a consolidação de diferentes relatos proto-biográficos em torno da imagem de Guimarães Rosa, romancista que, por sua vez, é artífice e demiurgo consciente de suas próprias personas (no sentido junguiano do termo), ou suas personagens de autoficção biográfica – categoria poética que solicita o aprofundamento de reflexões teórico-críticas. No presente caso, a notícia biográfica constrói-se com apoio em recursos poéticos, por meio de um discurso em que, articulando-se mutuamente, convergem literatura e jornalismo. Interroga-se, por fim, o papel do jornalismo enquanto construtor de personagens, em sua dimensão de representações sociais, como no caso da imagem de pessoas públicas, assim como as formas indiciais de indução ficcional que certos sujeitos podem exercer sobre os jornalistas e seus veículos, como forma de ampliar e consolidar a difusão de uma obra literária por meio de um personagem biográfico que seduz a atenção do leitor para muito além do último capítulo. Abstract: This article proposes to analyze the construction of characters of biographical fiction around the public figure of the writer João Guimarães Rosa, based on the confrontation of three journalistic articles dealing with the enigmatic death of the novelist, that occurred on November 19, 1967. It is worth mentioning that, in 2017, 50 years of this remarkable event of Brazilian culture are celebrated, still to be deciphered in all its scope and significance. From this bias, the present study also starts from the fact that Grande Sertão: Veredas is qualified, by its own author, as an "irrational autobiography": it is unprecedented in the history of literature to tell a life (and a death), and then live it. By this bias, the analysis focuses on the narrative dimension of the journalistic text, on the level of imbrications between the factual universe and the fictional universe (MOTTA, 2005). With a corpus of three articles published in O Globo, O Estado de São Paulo and Folha de São Paulo, the semantic-lexical indexes and the editorial constraints (CHARAUDEAU, 2010) are presented, which induce the consolidation of different protobiographical reports around the image of Guimarães Rosa, a novelist who, in turn, is a conscious artist and demiurge of his own personas (in the Jungian sense of the term), or his characters of biographical autofiction—a poetic category that calls for a deepening of theoretical-critical reflections. In the present case, the biographical news is constructed with support in poetic resources, through a discourse in which, by articulating each other, literature and journalism converge. Finally, the role of journalism as a character builder is questioned, in its dimension of social representations, as in the case of the image of public persons, as well as the indicial forms of fictional induction that certain subjects can exert on journalists and their vehicles as a way to broaden and consolidate the diffusion of a literary work through a biographical character that seduces the reader's attention well beyond the last chapter.

The objective of this study was to evaluate physiological and biochemical alterations during the development and storage of habanero pepper seeds with a view toward determining the time of harvest. Seeds were manually extracted from the fruit at three stages of development: E1 (fruit with first signs of yellowing), E2 (mature fruit) and E3 (mature fruit submitted to seven days of rest). After drying, seeds with 8% water content were stored at 10°C for 0, 4 and 8 months, and their quality evaluated by means of germination and vigor tests. Activities of the enzymes α-amylase, endo-β-mannanase, esterase, Superoxide Dismutase (SOD), malate dehydrogenase (MDH) and alcohol dehydrogenase (ADH) were evaluated during germination at 0, 48, 96 and 144 h after seeding. A randomized block design was used in a 3 × 3 factorial design (stages of development × storage) with 4 replications. Lower germination and vigor values were observed for the E1 stage seeds at all storage periods. In recently stored seeds, greater germination and vigor values were observed for the E3 stage seeds. Dormancy was observed principally in recently stored seeds and this was overcome at four months of storage. In summary, the physiological tests and activity of the enzymes evaluated indicated that the habanero pepper should be harvested at the E3 stage for a higher seed quality. Key words: Capsicum chinense, seed maturation, seed quality.

highlights Malnutrition during periods in development may have long-term effects in the adult. We examine parasitological behavior and histopathological. For the first time, this study describes the changes of the renal parenchyma. Induced by postnatal malnutrition associated with infection caused by Schistosoma mansoni. graphical a bstract abstract Effects of maternal malnutrition during lactation on the kidneys in mice infected with Schistosoma man- soni. Kidneys from programmed infected mice and their respective controls fed a normal diet (23% pro- tein), a protein-restricted group (PR) (8% protein) and a caloric-restricted group (CR) (according to the PR group intake) evaluated by biometry, morphometry and histopathology. Both PR and CR groups showed a reduction in the number of glomeruli when compared with the control group (CR: 29% vs C; PR: 41% vs C; p < 0.05) as well as infected mice (ICR: 32% vs IC; IPR: 47% vs IC; p < 0.05). Among infected mice, ICR group showed higher kidney weights (+18% vs IC and +12% vs IPR; p < 0.01). The ICR and IPR groups showed largest perimeter and area when compared to the corresponding uninfected group (ICR vs CR: +26%; IPR vs PR: +21%, p < 0.05) and area (ICR vs CR: +95%; IPR vs PR: +50%, p < 0.05). The ICR group showed an increase of within Bowman (CR vs ICR: +56%, p < 0.05), whereas Bowman's space was reduced (PR vs IPR, 61%; p < 0.05). Conclude that malnutrition during lactation programmed the metabolic state of the host, resulting in the evolution of the histology of the renal parenchyma.

Objetivou-se com este trabalho avaliar a qualidade sanitária e fisiológica de quatorze variedades de sementes de milho crioulas em pré e pós-armazenamento em embalagens de polietileno tereftalato (PET). Houve efeito das variedades e das épocas de avaliações sobre a incidência de fungos e germinação. Dentre a micobiota observada, Fusarium moniliforme (88%), Penicillium spp. (6,9%) e Aspergillus spp. (2,1%) tiveram a maior porcentagem de incidência. Após o armazenamento, houve decréscimo de F. moniliforme (69%) e aumento de Penicillium spp. (35%) e Aspergillus spp. (22%). A incidência desses fungos não comprometeu a qualidade fisiológica das sementes, que apresentaram elevadas porcentagens de vigor e germinação. The objective of this study was to evaluate the sanitary and physiological quality of fourteen varieties of creole corn seeds in pre-and post-storage in packaging of polyethylene terephthalate (PET). Among the mycobiota observed Fusarium moniliforme (88%), Penicillium spp. (6,9%) and Aspergillus spp. (2,1%) had the highest incidence. After storage there was a slight decrease in the percentage of F. moniliforme (69%) and a increase of Penicillium spp. (35%) and Aspergillus spp. (22%). The incidence of these fungi did not compromise the physiological quality of seeds that showed high percentages of vigor and germination.

Objetivou-se com este trabalho avaliar a qualidade sanitária e fisiológica de quatorze variedades de sementes de milho crioulas em pré e pós-armazenamento em embalagens de polietileno tereftalato (PET). Houve efeito das variedades e das épocas de avaliações sobre a incidência de fungos e germinação. Dentre a micobiota observada, Fusarium moniliforme (88%), Penicillium spp. (6,9%) e Aspergillus spp. (2,1%) tiveram a maior porcentagem de incidência. Após o armazenamento, houve decréscimo de F. moniliforme (69%) e aumento de Penicillium spp. (35%) e Aspergillus spp. (22%). A incidência desses fungos não comprometeu a qualidade fisiológica das sementes, que apresentaram elevadas porcentagens de vigor e germinação.The objective of this study was to evaluate the sanitary and physiological quality of fourteen varieties of creole corn seeds in pre-and post-storage in packaging of polyethylene terephthalate (PET). Among the mycobiota observed Fusarium moniliforme (88%), Penicillium spp. (6,9%) and Aspergillus spp. (2,1%) had the highest incidence. After storage there was a slight decrease in the percentage of F. moniliforme (69%) and a increase of Penicillium spp. (35%) and Aspergillus spp. (22%). The incidence of these fungi did not compromise the physiological quality of seeds that showed high percentages of vigor and germination.

Objective: To assess whether there has been any improvement in the quality and quantity of randomized controlled trials (RCTs) in nationally published journals through the application of standardized and validated scores. Methods: We electronically selected all RCTs published in the two indexed Brazilian journals that focus on orthopedics, over the period 2000-2009: Acta Ortopedica Brasileira (AOB) and Revista Brasileira de Ortopedia (RBO). These RCTs were identified and scored by two independent researchers in accordance with the Jadad scale and the Cochrane Bone, Joint and Muscle Trauma Group score. The studies selected were grouped as follows: 1) publication period (2000-2004 or 2004-2009); 2) journal of publication (AOB or RBO). Results: Twenty-two papers were selected: 10 from AOB and 12 from RBO. No statistically significant differences were found between the proportions (nRCT/nTotal of published papers) of RCTs published in the two journals (p = 0.458), or in the Jadad score (p = 0.722) and Cochrane score (p = 0.630). Conclusion: The relative quality and quantity of RCTs in the journals analyzed were similar. There was a trend towards improvement of quality, but there was no increase in the number of RCTs between the two periods analyzed.

Os objetivos deste trabalho foram avaliar a qualidade física, fisiológica e sanitária de sementes de 17 variedades de milho crioulo antes e após armazenagem em garrafas PET e comparar os resultados aos padrões já estabelecidos para comercialização de cultivares comerciais de milho. Foram realizados os testes de pureza física, retenção de peneiras, massa de 1000 sementes, grau de umidade, germinação, emergência em canteiro, índices de velocidade de germinação e emergência das sementes, além de exame de sementes infestadas. Pelos resultados obtidos, é possível concluir que as sementes de milho crioulo das diferentes variedades apresentam alta porcentagem de pureza física e germinação mesmo após o armazenamento, com valores acima dos padrões mínimos exigidos para comercialização de sementes de milho (98 e 85%, respectivamente). O grau de umidade das sementes das diferentes variedades de milho crioulo antes e após o armazenamento permaneceu abaixo de 13%, destacando-se a aptidão da região norte de Minas Gerais para a produção e conservação de sementes de qualidade. Três variedades apresentaram porcentagem de infestação superior ao padrão máximo recomendado (3%), sendo, portanto, necessário aprimorar o controle de pragas de grãos armazenados. São necessárias melhorias para uniformização de classificação das sementes por peneiras, já que as amostras de três variedades não alcançaram o padrão mínimo de 94% em uma mesma peneira, exigido pela legislação.The objective of this research was to evaluate the physical, physiological and sanitary quality of corn seeds of 17 maize varieties (landraces) before and after storage in PET bottles. The seeds were submitted to the following tests: physical purity, retention on sieves, weight of 1000 seeds, germination, seed moisture content, emergence in flowerbed, infested seed exam and seed health (deep-freezing method). Germination and emergence velocity index were determined. Maize landraces seeds of different varieties presented a great percentage of physical purity and germination, which were over the minimum standards values required for commercialization of corn seeds (98 and 85%, respectively), even after storage. Seed moisture content remained below 13% before and after storage, standing out the potential of Nothern Minas Gerais for production and conservation of high-quality seeds. Three varieties presented infestation higher than the maximum values accepted for corn seeds commercialization (3%), indicating the need of better pests control during storage. Samples of three varieties have not reached satisfy standard values of retention on the same sieve (94%), indicating the need of better standardization on size classification of some seed lots.

OBJETIVO: Verificar se houve melhora da qualidade e quantidade dos ensaios clínicos randomizados (ECRs) publicados na literatura nacional, por meio da aplicação de escores estruturados e validados na literatura. MÉTODOS: Selecionamos, eletronicamente, todas as edições das duas revistas indexadas e de escopo ortopédico da literatura nacional - Acta Ortopédica Brasileira (AOB) e Revista Brasileira de Ortopedia (RBO), no período de 2000-2009, todos os ECRs publicados. Dois pesquisadores independentes identificaram e pontuaram os ECRs de acordo com as escalas de JADAD e do grupo de trauma musculoesquelético da Colaboração Cochrane. Os trabalhos selecionados foram agrupados: 1) pelo período de publicação 2000-2004 e 2004-2009; 2) periódico de publicação (AOB e RBO). RESULTADOS: Vinte e dois trabalhos foram selecionados, sendo10 da AOB e 12 da RBO. Não houve diferença entre o número proporcional (nECR/nTotal de trabalhos publicados) de ensaios clínicos randomizados publicados nos dois periódicos (p = 0,458), assim como para os escores de JADAD (p = 0,722) e da Colaboração Cochrane (p = 0,630). CONCLUSÃO: A qualidade e quantidade relativa de ensaios clínicos randomizados nos periódicos analisados foi semelhante. Há uma tendência de melhora da qualidade; contudo, não há acréscimo da quantidade de ensaios clínicos randomizados nos dois períodos analisados. OBJECTIVE: To assess whether there was an improvement in the quality and quantity of randomized controlled trials (RCTs) in nationally published journals through an application of standardized and validated scores. METHODS: We selected, electronically, for the period of 2000-2009, all RCTs published at the two indexed, orthopaedics-focused Brazilian journals: Acta Ortopédica Brasileira(AOB) and Revista Brasileira de Ortopedia (RBO). These RCTs were identified and scored by two independent researchers according to the JADAD scale and Cochrane Bone, Joint and Muscle Trauma Group score. The selected studies were grouped: 1) by publication period (2000-2004 and 2004-2009); 2) journal of publication (AOB and RBO). RESULTS: Twenty-two papers were selected, 10 from AOB and 12 from RBO. No statistically significant differences were found between the proportion (nRCT/nTotal of published papers) of RCTs published in the two journals (p=0.458), as well as for the JADAD score (p=0.722) and Cochrane score (p=0.630). CONCLUSION: The quality and quantity of randomized clinical trials in the period was similar in the journals analyzed. There is a trend of improvement of quality, yet there was no increase in the number of randomized clinical trials in both periods. Universidade Federal de São Paulo (UNIFESP) Departamento de Ortopedia e Traumatologia Universidade Federal de São Paulo (UNIFESP) Departamento de Ortopedia e Traumatologia Setor de Ombro e Cotovelo da Disciplina de Mão e Membro Superior UNIFESP, Depto. de Ortopedia e Traumatologia UNIFESP, Depto. de Ortopedia e Traumatologia Setor de Ombro e Cotovelo da Disciplina de Mão e Membro Superior SciELO

CONTEXTO: O uso do pericárdio bovino como remendo na endarterectomia de carótida é uma alternativa à veia safena magna. As vantagens do pericárdio incluem facilidade de obtenção, menor tempo operatório e principalmente menor índice de ruptura. OBJETIVO: Avaliar a resistência tensional do pericárdio bovino tratado com glutaraldeído e compará-la com a da veia safena magna. MÉTODOS: Os remendos de pericárdio bovino (grupo I, n = 20) e de veia safena magna (grupo II, n = 20) foram recortados em dimensões iguais (50 x 5 mm) e preparados de modo habitual a sua utilização. Os grupos foram submetidos a ensaio de tração e comparados em relação a força de ruptura, força máxima e tensão de ruptura utilizando-se o teste t de Student. A correlação da espessura do remendo com a força de ruptura também foi analisada utilizando-se o coeficiente de correlação linear de Pearson. RESULTADOS: Os parâmetros força de ruptura e força máxima foram significativamente maiores no grupo dos remendos de pericárdio bovino: 1,97 versus 1,36 kgf (p = 0,001230) e 2,27 versus 1,51 kgf (p = 0,0001087), respectivamente. A tensão de ruptura média para o material pericárdio bovino também foi maior (193,99±43,05 versus 49,19±22,96 kgf/cm², p = 7,603e-16) do que na veia safena. A correlação entre a espessura e a força de ruptura foi considerada moderada (r = 0,5032993) para o pericárdio bovino e baixa (r = 0,3062166) para o grupo da veia safena. CONCLUSÃO: Os autores concluem que a resistência do pericárdio bovino à ruptura foi considerada adequada neste estudo, e é significativamente maior que a da veia safena magna, retirada da região da coxa. Além disso, a espessura do remendo em ambos os grupos não apresenta boa correlação com sua resistência a ruptura.BACKGROUND: Carotid endarterectomy using bovine pericardium is an acceptable alternative to great saphenous vein patch. Bovine pericardium is easily obtained and provides a shorter operative time and lower rupture rate. OBJECTIVE: To evaluate rupture resistance of glutaraldehyde-treated bovine pericardium patch in comparison with great saphenous vein patch. METHODS: The sample was divided into two groups: bovine pericardium patch (group I, n = 20) and great saphenous vein patch (group II, n = 20). Both bovine pericardium and saphenous vein patches were prepared in the same dimensions (50 mm x 5 mm) and tested using standard procedures. The patches were tested in the longitudinal axis until the point of material failure. The following parameters were addressed: failure force, ultimate force and failure stress. Statistical analysis was conducted using the Student t test and Pearson's linear correlation. RESULTS: Failure force and ultimate force parameters were significantly higher in the bovine pericardium patch group: 1.97 vs. 1.36 kgf (p = 0.001230) and 2.27 vs. 1.51 kgf (p = 0.0001087), respectively. Mean failure stress in the bovine pericardium patch group was also significantly higher than that in the great saphenous vein group (193.99±43.05 vs. 49.19±22.96 kgf/cm², p = 7.603e-16). The correlation between thickness and failure force was considered moderate (r = 0.5032993) for the bovine pericardium group and low (r = 0.3062166) for the great saphenous vein group. CONCLUSION: The failure stress related to the bovine pericardium group was considered appropriate in this study, and was significantly higher than that observed in the great saphenous vein group. In addition, patch thickness in both groups did not show a good correlation with rupture resistance.

Background: Following the publication of a prospective randomized trial (Endovascular Aneurysm Repair Trial 2 - EVAR2) that questioned the benefits of endovascular repair of abdominal aortic aneurysms (AAA) in high-surgical-risk patients, we decided to analyze our initial and long-term results with endovascular AAA repair in this patient population. Objective: To evaluate the operative mortality, long-term survival, frequency of secondary operations, outcome of the aneurysm sac, primary and secondary patency rates, and rupture rate after aortic stent-graft placement in high-surgical-risk patients. Methods: From April 2002 to February 2008, 40 high-surgical and anesthetic risk patients with an AAA managed by a bifurcated aortic endograft were entered in a prospective registry. Data concerning diagnosis, operative risk, treatment and follow-up were analyzed in all patients Results: Twenty-four Excluder® and 16 Zenith® stent-grafts were successfully implanted. Thirty patients (75%) were classed ASA III and 10 (25%) were ASA IV. Mean aneurysm diameter was 64 mm. Operative mortality was 2.5%. Two patients required reintervention during the mean follow-up of 28.5 months. Survival rate at 3 years was 95%. There were four endoleaks, one case of endotension, and one endograft limb occlusion. Primary and secondary patency rates at 3 years were 97.5 and 100%, respectively. There were no ruptures. Conclusions: Initial and long-term results with endovascular treatment of AAA in high-surgical-risk patients were satisfactory, and appear to justify such approach for this patient population.Contexto: Após a publicação de um estudo prospectivo e randomizado (Endovascular Aneurysm Repair Trial 2 - EVAR2) que questionou o benefício do tratamento endovascular de aneurismas da aorta abdominal (AAA) em pacientes de alto risco cirúrgico, decidimos avaliar nossos resultados iniciais e tardios neste grupo de pacientes. Objetivo: Avaliar a mortalidade perioperatória, a sobrevivência tardia, a freqüência das reintervenções, o comportamento dos sacos aneurismáticos, as patências primária e secundária e a incidência de rotura após o tratamento endovascular de AAA em pacientes de alto risco cirúrgico. Métodos: Entre abril de 2002 e fevereiro de 2008, 40 pacientes de alto risco anestésico-cirúrgico portadores de AAA foram submetidos ao implante de endopróteses bifurcadas de aorta e incluídos num registro prospectivo. Os dados a respeito do diagnóstico, risco operatório, tratamento e seguimento foram analisados em todos os pacientes. Resultados: 24 endopróteses Excluder® e 16 Zenith® foram implantadas com sucesso. Trinta pacientes (75%) foram classificados como ASA III e 10 (25%) como ASA IV. O diâmetro médio dos AAA era de 64 mm. A mortalidade perioperatória foi de 2,5%. Dois pacientes necessitaram de reintervenção durante o seguimento médio de 28,5 meses. A taxa de sobrevivência aos 3 anos foi de 95%. Houve quatro endoleaks, um caso de endotensão, e uma oclusão de ramo em uma endoprótese. As patências primária e secundária aos 3 anos foram de 97,5 e 100%, respectivamente. Não houve nenhuma rotura. Conclusões: Nossos resultados iniciais e tardios do tratamento endovascular de AAA em pacientes de alto risco são satisfatórios e parecem justificar a indicação deste tratamento neste grupo de pacientes.

Contexto: A arteriografia com contraste (AC) tem sido o exame tradicional de avaliação de pacientes com suspeita de doença oclusiva aorto-ilíaca (DOAI). Recentemente, métodos menos invasivos, como a eco-Doppler, têm sido usados com a mesma finalidade. Objetivo: Comparar prospectivamente a eco-Doppler com a AC e eventual manometria arterial direta (MAD) na avaliação pré-operatória de pacientes com suspeita de DOAI. Métodos: Foram submetidos a eco-Doppler e a AC 125 pacientes internados para tratamento de doença arterial oclusiva dos membros inferiores, avaliando comparativamente 552 segmentos da aorta infrarrenal e das artérias ilíacas comum e externa. As lesões encontradas foram classificadas em cinco categorias: 1) normal e estenose leve (0 a 19%); 2) estenose moderada (20 a 49%); 3) estenose significativa (50 a 79%); 4) estenose crítica (80 a 99%); e 5) oclusão total. A MAD foi usada em 19 segmentos de 15 pacientes para classificar lesões limítrofes entre duas categorias. Foram calculados índices de validade (sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e acurácia) para distinguir lesões hemodinamicamente significativas de não-significativas e para distinguir estenoses críticas de oclusões. O padrão-ouro foi AC, complementado pela MAD. Foram também calculados coeficientes de correlação kappa entre arteriografias e eco-Doppler para o conjunto dos segmentos aorto-ilíacos. Resultados: Lesões clinicamente relevantes (estenoses de 50 a 99% e oclusões totais) foram observadas na eco-Doppler em 163 segmentos (29,5%) e na AC em 158 segmentos (28,6%). A eco-Doppler mostrou altos índices de validade para distinguir lesões hemodinamicamente significativas de lesões não-significativas em todos os segmentos (acurácia = 92%; kappa = 0,81) e para diferenciar estenoses críticas de oclusões (acurácia = 86%; kappa = 0,73). Os índices de correlação entre os resultados das eco-Doppler e das AC foram ótimos em todos os segmentos aorto-ilíacos. Conclusão: A eco-Doppler apresenta elevados índices de validade e ótimos coeficientes de correlação com a AC na avaliação de pacientes com suspeita de DOAI.Background: Contrast arteriography (CA) has been the traditional method of evaluation of patients with suspected aortoiliac occlusive disease (AIOD). Recently, less invasive methods, such as Doppler ultrasonography, have been used for the same purpose. Objective: The present study prospectively compares Doppler ultrasonography with CA and direct arterial manometry (DAM) in the preoperative evaluation of patients with suspected AIOD. Methods: A total of 125 patients admitted for treatment of arterial occlusive disease of the lower extremities underwent Doppler ultrasonography and CA, comparatively evaluating 552 aortic, common iliac e external iliac segments. The lesions found were classified into five categories: 1) normal and mild stenosis (0-19%); 2) moderate stenosis (20-49%); 3) significant stenosis (50-79%); 4) critical stenosis (80-99%); and 5) total occlusion. DAM was used in 19 segments of 15 patients to classify borderline lesions. Validity indexes (sensitivity, specificity, positive predictive value, negative predictive value and overall accuracy) were calculated to distinguish hemodynamically significant from non-significant stenosis and to discriminate critical stenosis from total occlusions. The gold standard was CA, supplemented by DAM. Correlation coefficients (kappa statistics) between arteriography and Doppler ultrasonography were also calculated for the whole sample of aortoiliac segments. Results: Clinically relevant lesions (stenoses between 50-99% and total occlusions) were observed on Doppler ultrasonography in 163 segments (29.5%) and on CA in 158 segments (28.6%). Doppler ultrasonography showed high validity indexes to distinguish hemodynamically significant from non-significant lesions (overall accuracy = 92%; kappa = 0.81) and an overall accuracy of 86% (kappa = 0.73) to distinguish critical stenosis from total occlusion. Optimal correlation coefficients between the results of Doppler ultrasonography and CA were observed for all aortoiliac segments. Conclusion: Doppler ultrasonography has high validity indexes and optimal correlation coefficients with CA in the evaluation of patients with suspected AIOD.

Este trabalho parte da consideração do envelhecimento humano como experiência heterogênea. Busca pôr em diálogo o grupo de sala de espera, recorrendo ao construcionismo social, para descrevê-lo como prática discursiva. Problematizando essa modalidade de intervenção psicológica, nesse estudo objetivamos descrever o processo de produção de sentidos sobre o envelhecimento humano nesse contexto. Dez grupos de sala de espera, gravados e transcritos, foram pré-analisados favorecendo a escolha de um deles para análise extensiva. Essa análise apontou que, nesse processo conversacional, a vivência do envelhecimento como experiência individual vai sendo paulatinamente reconstruída pelos interlocutores como de natureza sócio-interacional, apontando para posições mais ativas na produção de qualidade de vida no envelhecimento. Assim, os participantes em co-autoria, falando de seus cotidianos, abrem possibilidades de novas descrições sobre si mesmos enquanto pessoas idosas, nesse contexto interativo. This research question the human aging while universal experience. Using the social construcionism, the waiting room group is understood as a discursive production, having the language as a main point of social practices and focalizing a relational perspective in the producing process of the world meanings. Thinking about this psychological intervention, we have the purpose of describing the meaning production process about the human aging in this context. Ten waiting room groups, recorded and transcribed, were analyzed, what made possible the selection of one of them for extensive analyses. Their analysis show that in this conversation the aging experience like an individual experience is being changed by the speakers as social interaction, pointing to actives positions on healthy life quality aging production. The speakers construct several meanings about aging, talk about themselves in their daily relationships, creating the possibility for new descriptions about themselves as elders in this interactive context.

O objetivo deste trabalho foi avaliar os padrões eletroforéticos das proteínas resistentes ao calor e a atividade da enzima endo-β-mananase durante a germinação de sementes de alface, em alta temperatura. Sementes de oito cultivares de alface foram submetidas aos testes de germinação, primeira contagem e emergência em duas temperaturas, 20 e 35ºC. Foram calculados o índice de velocidade de germinação (IVG) e o índice de velocidade de emergência (IVE). Avaliou-se, também, a expressão das proteínas resistentes ao calor e da enzima endo-β-mananase, para todos os tratamentos. Utilizou-se o delineamento experimental inteiramente casualizado, em arranjo fatorial 2x8, com duas temperaturas e oito cultivares. A maior germinação é observada a 35ºC na cultivar Everglades, considerada termotolerante. Os padrões de proteínas resistentes ao calor em sementes de alface apresentam bandas específicas na cultivar Everglades, a 35ºC. A atividade da enzima endo-β-mananase é maior na cultivar Everglades, nessa temperatura. Essa cultivar tem potencial para utilização em programas de melhoramento de alface com vistas à tolerância a altas temperaturas durante a germinação. The objective of this work was to evaluate the electrophoretic patterns of heat-tolerant proteins and the activity of the endo-β-mannanase enzyme during lettuce seed germination, at high temperature. Seeds of eight lettuce cultivars were subjected to germination, first count, and emergence tests at two temperatures, 20 and 35ºC. The index of germination speed (IVG) and the emergence rate index (IVE) were calculated. The expression of heat-tolerant proteins and of the endo-β-mannanase enzyme was also evaluated for all treatments. The experimental design was completely randomized, in a 2x8 factorial arrangement, with two temperatures and eight cultivars. The highest germination is observed at 35ºC in the Everglades cultivar, considered thermotolerant. The patterns of heat-tolerant proteins in lettuce seeds present specific bands in the Everglades cultivar, at 35ºC. The activity of the endo-β-mannanase enzyme is higher in the Everglades cultivar, at this temperature. This cultivar has potential for use in lettuce breeding programs aiming for tolerance to high temperatures during germination.