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Systematic review was conducted to determine the state of mushroom related indigenous knowledge in Southern Africa. Fifty-six edible species of mushrooms were recorded out of 266 macrofungi species in the region. Of this number of species, South Africa accounted for 221 species (not exclusive count), Zimbabwe (21), Mozambique (18), Namibia (14), Botswana (10), Zambia (7), and Mauritius (1). The most abundant genera based on the number of species were Agaricus, Lepiota, Cantherellus, Peziza, Termitomyces, Calvatia, Lycoperdon, Amanitia, Coprinus and Coprinellus. The most popular species used for food only (by ≥3 tribes/localities) included Amanita masasiensis, Amanita zambiana, Cantharellus cf. floridula, Cantherellus heimannianu), Cantharellus isabellinus, Cantherellus miomboensis, Cantharellus symoensii, Lactiflius edulis. Seven dual purpose or medicinal mushroom species: Agaricus bisporus, Hericium erinaceus, Kalaharituber pfeilii, Pleurotus ostreatus, Schizophyllum commune, Termitomyces schimperi and Volvariella volvocea were recorded. Only G. lucidum was used solely for medicinal purposes. Popular mushroom species (based on ≥3 countries) included Kalaharituber pfeilii, Pleurotus ostreatus, Schizophyllum commune and Terfezia pfeilii. Much work still needs to be done on these mushrooms.

Baseline data on the boreal jack pine associated chanterelle (Cantharellus cibarius Fr.) and pine mushrooms (Tricholoma magnivelare (Peck) Redhead) in the Boreal Plain Ecozone of Saskatchewan were collected in five ecosites of productive mushroom areas. It investigated hourly weather parameters correlated with daily purchase volumes over 4 yrs; yield data over 5 yrs; and varying age, tree density, and species for presence of mushrooms in over 100 stands. All plots fell within the lichen jack pine (jP) ecosite with an overstory entirely of jack pine. The understory was dominated by reindeer lichen, bearberry, and blueberry. Plots were well- to rapidly drained, subxeric to submesic, with low nutrient regime. Both chanterelle and pine mushrooms were present in jack pine stands of −1. Chanterelle cap diameter was a good predictor of fresh weight and proxy for yield. Pine mushroom was −1 over 4 yrs based on 5 days picking. With future climate predictions of warmer and moister conditions, timing of fruiting body appearance is anticipated to advance.

Introduction. Cantharellus cibarius L. is a wild mushroom that has been part of human diet for many centuries. However, there is little reliable information about its nutritional value, storage conditions, shelf life, and processing. The research objective was to study the nutritional value of C. cibarius growing in West Siberia, as well as to define its storage and processing conditions. Study objects and methods. The research featured fresh and processed (boiled and salted) wild chanterelles (C. cibarius) obtained from the forests of the Novosibirsk region. The mushrooms were tested for amino acids, fatty acids, nutrients, reducing sugars, trehalose, mannit, glycogen, fiber, mucus, squalene, ash, minerals, vitamins, trypsin inhibitor, chlorides, mesophilic and facultative anaerobes, etc. The samples also underwent sensory evaluation. Results and discussion. The samples of C. cibarius proved to have a high nutritional value. The samples contained 3.6% proteins, including essential amino acids; 3.9% carbohydrates, including sugars and dietary fiber; and 0.7% lipids, including saturated, monounsaturated, and polyunsaturated acids. In addition, C. cibarius appeared to be rich in biologically active substances. It contained trypsin inhibitors that reduce the absorption of protein compounds. Purchasing centers can be recommended to use 70–80% relative air humidity. At 0–2°C, the storage time was five days; at 5–10°C – three days; at 15–20°C – two days; at 20–30°C – one day. Before processing, the mushrooms were washed twice in non-flowing water. C. cibarius also proved to be a valuable raw material for boiled and salted semi-finished products. The optimal boiling time was 5–10 min. Lightly-, medium-, and strong-salted semi-finished mushrooms were ready for consumption after the fermentation was complete, i.e. after day 15, 10, and 3, respectively. Conclusion. Boiled and salted semi-finished products from Siberian C. cibarius demonstrated excellent sensory qualities and can become part of various popular dishes.

Lignocelulozni materiali so obnovljivi naravni vir, ki ga uporabljajo na različnih tehnoloških področjih. Težavna razgradnja lignina predstavlja oviro pri izkoriščanju ligninoceluloznega materiala. Kraljestvo gliv je bogat in neizkoriščen vir biotehnološko zanimivih encimov, med katerimi so tudi encimi, ki omogočajo razgradnjo lignina. Glavni encimi, ki sodelujejo pri razgradnji lignina so lignolitične oksidoreduktaze (lakaze, lignin peroksidaze, ter mangan peroksidaze). Cilj magistrske naloge je bil pripraviti vodne esktrakte 62 gliv nabranih v slovenskih gozdovih, ter s pomočjo testa oksidacije ABTS odkriti, ali vodni ekstrakti gliv vsebujejo encime, ki omogočajo razgradnjo lignina. Identificirali smo željene encimske aktivnosti v nekaterih vrstah gliv, ki še niso bile opisane v literaturi. Najvišje encimske aktivnosti smo določili pri glivah rodu Cantharellus, pri katerih smo podrobneje preverili tudi vpliv pH na encimsko aktivnost. Ugotovili smo, da imajo lakaze, lignin peroksidaze in mangan peroksidaze optimum delovanja večinoma v kislem in nevtralnem območju pH. Lignocelullosic material is renewable natural resource, that is used in different technological areas. Difficult degradation of lignin presents an obstacle for utilization of lignocelullosic material. The kingdom of fungi is rich and unexploited source of biotechnologically interesting enzymes, including lignin-degrading enzymes. Main lignin-degrading enzymes are ligninolytic oxidoreductases (laccases, lignin peroxidases, and manganese peroxidases). The aim of this master thesis was to prepare water extracts of 62 fungi from Slovenian forests and using ABTS oxidation test discover potential lignin-degrading enzymes. In certain fungal species we identified desired enzymatic activities, that were not yet described in the literature. We determined the highest enzymatic activities in fungi from the genus Cantharellus, for which we investigated the effect of pH on enzymatic activity in more detail. We found out that laccase, lignin peroxidase and manganese peroxidase have optimum activities in the acidic and neutral pH range.

Mushrooms are appreciated not only for their nutritional value and bioavailability, but also for their medicinal properties. There are a growing number of the studies focused on investigating medical preparation base on natural compounds, including those isolated from a number of fungi species of fungi species. The aim of this study is to compare anti-inflammatory, analgesic and antiulcer effects of extracts of shiitake mushrooms and chanterelle mushrooms in an experiment on rats. Materials and methods. The experiment involved 30 white female rats weighing 170-210 g., divided into 5 groups: group 1 included intact animals; group 2 included animals with modelled pathology; group 3 included animals, which received chanterelle extract; group 4 involved animals, which received shiitake mushroom extract, and group-5 rats received the reference drug. Observation of changes over the inflammatory process in the model of carrageenan-induced inflammation in the paw of the rats revealed an anti-exudative effect of varying intensity that made up 36.84% for the of chanterelle mushroom extract and 15.79% for shiitake mushroom extract. The dynamics of trypsin-induced inflammatory process showed moderate anti-inflammatory activity, 32.02%, during the courses of the therapy with chanterelle mushroom extract. According to the indicator of analgesic effects produced by the studied extracts, the highest indicator was reached by the chanterelle mushroom extract (33.53%) that exceeded the analgesic effect of the shiitake mushroom extract (17.65%). Therapeutic and prophylactic administration of the chanterelle mushroom extract in a dose of 10 mg / kg in the model of aspirin-induced gastric ulcer in rats showed a pronounced anti-ulcer effect which made up 66.7% compared with that by shiitake mushroom extract (33.4%) and the reference drug “Altan” (100%). Conclusion. The obtained data have demonstrated the promise of using the extracts of chanterelle and shiitake mushrooms for the therapeutic and prophylactic purposes.

The radioactive isotope, 40K, of naturally occurring potassium (0.012%) is present in the Earth’s crust in a low percentage of all potassium, leading to its presence in almost all foodstuffs. The impact of 40K activity concentrations was assessed in wild and cultivated edible mushrooms and in growing substrates. Samples were analysed by gamma spectroscopy. In the wild mushroom species, the average activity concentration of 40K was 1291 Bq kg−1 dry weight (dw), approximately 140 Bq kg−1 fresh weight (fw), with a range of average values per species from 748 in Lactarius deliciosus to 1848 Bq kg−1 dw in Tricholoma portentosum. The cultivated species presented an average value of 1086 Bq kg-1 dw; and the soils, compost of cultivation and wood of substrate are 876, 510 and 59.4 Bq kg−1 dw, respectively. The total K content reached a maximum of 59,935 mg kg−1 dw in T. portentosum. The transfer factors (TF > 1) suggested that mushrooms preferentially bioconcentrated 40K. Cantharellus cibarius, Craterellus tubaeformis, Hydnum repandum and T. portentosum by most TF could be considered as bioindicators of 40K. Taking into account that the annual radiation dose of 40K due to the average consumption of mushrooms analysed (0.15 μSv/year) is very low, it can be concluded that the consumption of these mushrooms does not represent a toxicological risk for human health. Finally, according to the total K content, from the nutritional point of view, these mushrooms could be considered as a potential source of potassium for the human diet.

In this study, dry matter, total ash, total phenolic content, antioxidant capacities, antimicrobial activity, and phenolic acid contents of nine different mushrooms (Lactarius deliciosus, Agaricus bisporus, Lentinus edodes, Pleurotus ostreatus, Ganoderma lucidum, Hericium erinaceus, Morchella spp., Boletus edulis, Cantharellus cibarius) from Turkey were determined. Water (W), methanol (M), and water-methanol mixture (W/M, 50:50 v/v) were used to extract phenolic substances from dried mushroom samples. Generally, W extracts showed higher values in total phenolic content and antioxidant capacity values. W extracts of B. edulis (17.11 mg/g), Morchella spp. (14.19 mg/g). Moreover, P. ostreatus (10.85 mg/g) had the highest phenolic contents (p < 0.05). The lowest antioxidant capacity values were found in W/M extracts of C. cibarius (20.22 mu mol/g), and L. deliciosus (28.77 mu mol/g) and M extract of P. ostreatus (31.61 mu mol/g) mushrooms (p < 0.05). W and W/M extracts were more efficient in inhibiting Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, and Staphylococcus aureus than M extracts. Besides, B. edulis and C. cibarius species were found to have the strongest antimicrobial activity. Besides, B. edulis and P. ostreatus have been found to contain the highest phenolic acid compounds (p < 0.05), supporting the high content of total phenolic contents and antioxidant properties. This study revealed that these mushrooms have the potential to be used in the food and pharmaceutical industries.

Two new species of yellow Cantharellus and a new record of Cantharellus tabernensis associated with tropical species of Quercus are presented, based on the taxonomic study of fresh specimens and in a phylogenetic analysis of transcription elongation factor 1-alpha (tef-1α) and the large subunit of the ribosome (nLSU) sequences. One of the new species proposed here, corresponds to a choice edible mushroom, which, in our molecular phylogeny, resulted in it being related to the group of species around C. lateritius and sister with Craterellus confluens type specimen. This latter is here formally transferred to Cantharellus and consequently a new name, Cantharellus furcatus, is proposed to replace the homonym Cantharellus confluens (Schwein.) Schwein. 1834 a later synonym of Byssomerulius corium. Detailed macroscopic and microscopic descriptions accompanied with illustrations and a taxonomic discussion are presented for each species.

Cantharellus macrocarpus, a member of Cantharellus subgenus Cantharellus, is described as a new species from Hainan Province, China. It is characterized by large basidioma with a bright yellow-orange pileus and a well-developed, rugulose hymenophore, cream to pale yellowish stipe, noticeably thickened hyphal walls in the pileipellis, presence of clamp connections in all parts of the basidiomata, and a distribution in tropical Asia. A phylogenetic analysis of DNA sequences from part of the 28S gene and the translation elongation factor 1-a gene (TEF1) confirmed that it forms an independent lineage within subgenus Cantharellus. A detailed description, color photos of fresh basidiomata and line drawings of micromorphological features are presented.

In this third contribution involving new Cantharellus species from South Korea, two new species are introduced. In addition, we document a first report of the recently described Japanese Cantharellus anzutake outside of Japan based on identical ITS sequence data. Cantharellus citrinussp. nov. is introduced as a new member of subgenus Cinnabarini, to which the closely related Korean C. albovenosus and Chinese C. phloginus also belong. Cantharellus curvatussp. nov. is introduced as a new member of subgenus Parvocantharellus, in which the Korean C. koreanus was recently placed. The respective placements of the new taxa are significantly supported by a phylogenetic analysis of sequences from the transcription elongation factor (tef-1).

A new species, Cantharellus albus, was collected from southwestern China and described with both morphological and molecular characters. It differs from other species of Cantharellus by its smooth white pileus, white hymenophore, and white stipe, the latter turning yellow after bruising. The phylogenetic tree inferred from the combined dataset of three DNA fragments, namely large subunit ribosomal RNA gene (LSU), RNA polymerase II second largest subunit (rpb2), and translation elongation factor 1-alpha gene (tef1), indicated that the new species is distinct from other species of Cantharellus and is a member of sect. Flavobrunnei within the subgenus Parvocantharellus.

Fungal species of Cantharellales order are found worldwide including Europe, Africa, Asia, and North America. This clade includes two smaller, separate, but closely related genera Cantharellus and Craterellus. Cantharellus was firstly identified in the middle of the 17th century. Besides traditional classification based on morphology, molecular phylogeny of the nuclear ribosomal large subunit gene (nrLSU) can be an effective tool to provide more information during species identification for this group. In this current report, a dataset of the nrLSU gene including 60 sequences of Cantharellus species and 1 sequence belonging to Craterellus (outgroup), was obtained from Genbank. Phylogenetic analyses by MEGA 6.0 showed a monophyletic relationship between the six well-established clades, namely Cantharellus (Clade 1), Rubrinus (Clade 2), Cinnabarinus (Clade 3), Parvocantharellus (Clade 4), Pseudocantharellus (Clade 5) and subgenus Afrocantharellus (Clade 6) similar to that of morphological classification. Therefore, this dataset is efficient to support the identification of the fungal samples, collected at the Langbian Mountain, Lam Dong.

The paper analyzes plant communities with Cantharellus cibarius Fr. in southern taiga and sub-taiga forests in the Kirov Region. Golden chanterelle occurs mostly in pure pine forests and pine forests mixed with spruce and birch of green-moss, lichen and cowberry types in young to maturing stands; the crown density is low or medium. Species richness of herbaceous-shrub storey in the studied communities varied from 5 to 29. The ecological preferences of C. cibarius, defined with Ellenberg (1974) scales, allow to characterize the species in the following way: temperate climate species, shade-resistant, rarely found in conditions of total shading; mesophyte regarding soil humidity, prefers acidic soils with low nitrogen, but, as an exception, marked on neutral soils rich in nitrogen. The differences in the species ecological preferences in conditions of southern taiga and sub-taiga are insignificant. The hemeroby index varied from 0,05 to 0,33 for the studied communities. The average share of species tolerant to human impact was 13,23%, and the share of anthropophobic species – 86,77%. These data characterise C. cibarius as the species capable of tolerating moderate human impact in southern taiga subzone, but being less tolerant to human impact in sub-taiga areas and coniferous-broadleaved forests.

For the accurate quantitation of kokumi-enhancing and bitter-tasting octadecadien-12-ynoic and octadecadienoic acids in chanterelles (Cantharellus cibarius Fr.), a sensitive ultra-high-performance liquid chromatography-differential ion mobility spectrometry-tandem mass spectrometry method was developed. On the basis of these quantitative data and the taste thresholds, dose-over-threshold factors were calculated to determine the contribution of these sensometabolites to the kokumi and bitter taste of chanterelles; e.g., 14,15-dehydrocrepenynic acid (3) and (9Z,15E)-14-oxooctadeca-9,15-dien-12-ynoic acid (7) were identified as key kokumi substances in raw chanterelles. Quantitative profiling of these compounds in various mushroom species demonstrated a unique accumulation of octadecadien-12-ynoic acids in Cantharellus. Furthermore, storage experiments highlighted dynamic processes, including the biosynthesis of these substances as a result of lipid peroxidation mechanisms.

The aim of the study was to calculate the content of non-freezing water (NFW) as well as amount of water associated with the evaporation process (Nev) in the chosen species of fungi. The study focused on lyophilized in vitro mycelium and commonly cultivated mushrooms Agaricus bisporus, Boletus badius and Cantharellus cibarius. Both NFW and Nev were examined by means of differential scanning calorimetry (DSC). The above-mentioned types of water are important components of the state diagram that is a kind of functional graph helping in identifying food stability during storage and selecting suitable conditions of temperature and moisture content for processing. The content of non-freezing water in all examined samples ranged between 0.19 and 0.31 g g−1. The estimated amount of water associated with the evaporation process was found to be between 0.05 and 0.13 g g−1. The obtained results were variable and highly dependent on fungus species, origin (in vitro mycelium vs cultivated) and content of accumulated metals. The influence of these factors is discussed. In order to determine statistical significance, selected samples were measured at least 5 times; the relative standard of deviation (%RSD) did not exceed 4.45 of measured enthalpies. In the group of mycelium from in vitro cultures, the DSC method was used for the first time. The publication also compared the NFW and Nev values with those obtained for naturally grown fruiting bodies of the same species.

The antioxidant properties of Boletus edulis, Cantharellus cibarius and Craterellus cornicupioides decoctions and the effect of mushroom addition on the total phenolic content and the degree of secondary oxidative changes on lipids in frankfurters was studied. Moderate antioxidant activity was obtained by DPPH assay when mushroom decoctions were tested in vitro. Using the conjugated diene method, moderate antioxidant activity was achieved with Boletus edulis and Craterellus cornicupioides decoction, while with Cantharellus cibarius decoction, antioxidant activity was low. Constant amounts of phenolic acid were obtained in frankfurters fortified with Boletus edulis, while lipid oxidation on each tested day was several times less than in the control group of frankfurters, throughout two months of refrigerated storage. Generally, these mushrooms could be used as natural antioxidants to interfere with the chemical deterioration of food products and specifically, to extend the shelf life of cooked pork sausages.

Angiogenesis is a complex physiological process that cannot be treated with single agent therapy. Several edible fungi have been known to encompass bioactive compounds, and are promising sources of multi-component drugs. One such widely consumed edible fungi is Cantharellus cibarius, which has been explored for its biological activities. The present study focused on assessing the anti-angiogenic activity of petroleum ether and ethanol extracts of C. cibarius using chick chorioallantoic membrane (CAM) assay. Both the extracts showed a dose-dependent response which was compared with the anti-angiogenic activity of the positive controls silibinin, and lenalidomide. The extracts were also studied for their lipoxygenase (LOX) inhibitory potential and compared to ascorbic acid as the positive control. The IC50 values of the petroleum ether extract, ethanol extract, and ascorbic acid for LOX inhibition assay were 135.4, 113.1, and 41.5 µg/mL, respectively. Although both the extracts showed similar responses in CAM assay, ethanol extract proved to be more potent in LOX inhibition assay. Finally, the extracts were investigated for their chemical composition using GC-MS. A correlation between LOX inhibition and anti-angiogenic potential was established at the molecular level. A meticulous literature search was carried out to correlate the biochemical composition of the extracts to their anti-angiogenic activity.

In this study, variation of some chemical components such as anthocyanin, β-carotene, lycopene, phenolic, nitrate, soluble protein, proline, glucose, sucrose and total carbohydrate level ad PAL activity in some wild and cultivated edible mushrooms was examined. For this, four different mushroom species (Agaricus campestris L., Cantharellus cibarius Fr., Hericium erinaceus (Bull.) Pers., Lactarius piperatus L. Pers) were supplied from local market, named Kuzeykent Semt Bazaar, in Kastamonu province of Turkey. Mushroom samples were collected from Araç, Daday, Devrekani and Tosya locations of Kastamonu. According to findings, the highest anthocyanin value and PAL activity were obtained from A. campestris collected from Arac location with 0.107 mg g-1 and 6.99 EU, respectively. The amount of β-carotene (2.297 mg g-1) and lycopene (0.644 mg g-1) was the highest in C. cibarius collected from Tosya location, however; proline, soluble protein, nitrate and glucose level were the maximum in A. campestris collected from Devrekani location with 149.61 µmol g-1, 55.49 mg, 159.963 mg g-1 and 29.36 µg g-1, respectively. While total carbohydrate was the highest in H. erinaceus collected from Araç location with 80.97 µg g-1, sucrose concentration was the maximum with 39.22 mg g-1 in H. erinaceus collected from Daday location. As a result, A. campestris collected from Devrekani location exhibited the highest nutrient in terms of chemicals analysed except anthocyanin and it was followed by H. erinaceus collected from Daday location. However, C. cibarius and H. erinaceus collected from Araç location had lower chemical components. It can be said that these mushroom species are valuable and important as major food sources and non-wood products for Kastamonu province.

Mushrooms are able to accumulate toxic trace elements. This study investigates the content of cadmium (Cd) and lead (Pb) in selected species of fungi (Boletus badius, Boletus edulis, and Cantharellus cibarius) from the northeastern part of Poland and estimates their edible safety. The amount of Cd and Pb was determined by flameless atomic spectrometry using the iCE 3000 Series-Thermo. The mean content of Cd in analyzed mushrooms ranged from 0.370 to 2.151 mg/kg d.w., while Pb was found at the level of 0.243–0.424 mg/kg d.w. Boletus edulis was characterized by the highest content of Cd, whereas Cantharellus cibarius contained the biggest amount of Pb. Estimated exposure to the Cd intake expressed as percentage share in TWI (Tolerable Weekly Intake) was at the highest level in Boletus edulis (30.87%), which could be associated with the risk of excessive Cd accumulation in the body.

The genus Cantharellus, commonly known as chanterelles, has recently been divided into six subgenera, however, wider sampling approaches are needed to clarify the relationships within and between these groups. A phylogenetic overview of Cantharellus subgenus Parvocantharellus in China was inferred based on the large subunit nuclear ribosomal RNA gene (nrLSU), the DNA-directed RNA polymerase II subunit 2 (rpb2), and the transcription elongation factor 1-alpha (tef1). A total of nine species from China were assigned to the subgenus, including seven novel species, namely Cantharellusaurantinus, C. austrosinensis, C. galbanus, C. luteolus, C. luteovirens, C. minioalbus, and C. sinominior, and two known species, namely C. albus and C. zangii. The detailed descriptions and illustrations were provided based on the newly obtained data, with the comparisons to closely related species. C. zangii was restudied based on the paratype specimens and multiple new collections from the type locality. Futhermore, the Indian species C. sikkimensis was identified as a synonym of C. zangii based on the morphological and molecular analyses. A key to the Chinese species belonging to the subg. Parvocantharellus is also provided.

Edible mushrooms have been recognized as a highly nutritional food for a long time, thanks to their specific flavor and texture, as well as their therapeutic effects. This study proposes a new, simple approach based on FT-IR analysis, followed by statistical methods, in order to differentiate three wild mushroom species from Romanian spontaneous flora, namely, Armillaria mellea, Boletus edulis, and Cantharellus cibarius. The preliminary data treatment consisted of data set reduction with principal component analysis (PCA), which provided scores for the next methods. Linear discriminant analysis (LDA) managed to classify 100% of the three species, and the cross-validation step of the method returned 97.4% of correctly classified samples. Only one A. mellea sample overlapped on the B. edulis group. When kNN was used in the same manner as LDA, the overall percent of correctly classified samples from the training step was 86.21%, while for the holdout set, the percent rose to 94.74%. The lower values obtained for the training set were due to one C. cibarius sample, two B. edulis, and five A. mellea, which were placed to other species. In any case, for the holdout sample set, only one sample from B. edulis was misclassified. The fuzzy c-means clustering (FCM) analysis successfully classified the investigated mushroom samples according to their species, meaning that, in every partition, the predominant species had the biggest DOMs, while samples belonging to other species had lower DOMs.

White chanterelles (Basidiomycota), lacking the orange pigments and apricot-like odour of typical chanterelles, were found recently in the Canadian provinces of Québec (QC) and Newfoundland & Labrador (NL). Our phylogenetic analyses confirmed the identification of all white chanterelles from NL and QC as Cantharellus enelensis; we name these forma acolodorus. We characterized carotenoid pigments, lipids, phenolics, and volatile compounds in these and related chanterelles. White mutants of C. enelensis lacked detectable β-carotene, confirmed to be the primary pigment of wild-type, golden-orange individuals, and could also be distinguished by their profiles of fatty acids and phenolic acids, and by the ketone and terpene composition of their volatiles. We detected single base substitutions in the phytoene desaturase (Al-1) and phytoene synthase (Al-2) genes of the white mutant, which are predicted to result in altered amino acids in their gene products and may be responsible for the loss of β-carotene synthesis in that form.

Cantharellus cibarius is a widely distributed, popular, edible fungus with high nutritional and economic value. However, significant challenges persist in the microbial ecology and artificial cultivation of C. cibarius. Based on the 16S rRNA sequencing data, this study analyzed bacterial community structures and diversity of fruit bodies and rhizomorph parts of C. cibarius and mycosphere samples (collected in the Wudang District, Guiyang, Guizhou Province, China). It explored the composition and function of the core bacterial taxa. The analyzed results showed that the rhizomorph bacterial community structure was similar to mycosphere, but differed from the fruit bodies. Members of the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium complex had the highest abundance in the fruit bodies. However, they were either absent or low in abundance in the rhizomorphs and mycosphere. At the same time, members of the Burkholderia-Caballeronia-Paraburkholderia complex were abundant in the fruit bodies and rhizomorphs parts of C. cibarius, as well as mycosphere. Through functional annotation of core bacterial taxa, we found that there was an apparent trend of potential functional differentiation of related bacterial communities in the fruit body and rhizomorph: potential functional groups of core bacterial taxa in the fruit bodies centered on nitrogen fixation, nitrogen metabolism, and degradation of aromatic compounds, while those in rhizomorphs focused on aerobic chemoheterotrophy, chemoheterotrophy, defense against soil pathogens, decomposition of complex organic compounds, and uptake of insoluble inorganic compounds. The analysis of functional groups of bacteria with different structures is of great significance to understand that bacteria promote the growth and development of C. cibarius.

Mitochondrial genes and genomes have patterns of inheritance that are distinctly different from those of nuclear genes and genomes. In nature, the mitochondrial genomes in eukaryotes are generally considered non-recombining and homoplasmic. If heteroplasmy and recombination exist, they are typically very limited in both space and time. Here we show that mitochondrial heteroplasmy and recombination may not be limited to a specific population nor exit only transiently in the basidiomycete Cantharellus cibarius and related species. These edible yellow chanterelles are an ecologically very important group of fungi and among the most prominent wild edible mushrooms in the Northern Hemisphere. At present, very little is known about the genetics and population biology of these fungia cross large geographical distances. Our study here analyzed a total of 363 specimens of edible yellow chanterelles from 24 geographic locations in Yunnan in southwestern China and six geographic locations in five countries in Europe. For each mushroom sample, we obtained the DNA sequences at two genes, one in the nuclear genome and one in the mitochondrial genome. Our analyses of the nuclear gene, translation elongation factor 1-alpha (tef-1) and the DNA barcode of C. cibarius and related species, suggested these samples belong to four known species and five potential new species. Interestingly, analyses of the mitochondrial ATP synthase subunit 6 (atp6) gene fragment revealed evidence of heteroplasmy in two geographic samples in Yunnan and recombination within the two new putative species in Yunnan. Specifically, all four possible haplotypes at two polymorphic nucleotide sites within the mitochondrial atp6 gene were found distributed across several geographic locations in Yunnan. Furthermore, these four haplotypes were broadly distributed across multiple phylogenetic clades constructed based on nuclear tef-1 sequences. Our results suggest that heteroplasmy and mitochondrial recombination might have happened repeatedly during the evolution of the yellow chanterelles. Together, our results suggest that the edible yellow chanterelles represent an excellent system from which to study the evolution of mitochondrial-nuclear genome relationships.

In this study, DPPH free radical activity, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of wild edible mushroom Cantharellus cibarius Fr. collected from Antalya (Turkey) province were determined. Ethanol (EtOH), methanol (MeOH) and dichloromethane (DCM) extracts of C. cibarius mushroom were obtained using soxhlet apparatus. TAS, TOS and OSI values were determined by using Rel Assay kits. Free radical scavenging activity was determined using DPPH method. As a result of the studies, TAS value of the mushroom was found as 5.268±0.059 mmol/L, TOS value was 6.380±0.256 μmol/L and OSI value was 0.121±0.005. DPPH free radical activity was determined as EtOH extract 70.52±0.50, MeOH extract 64.34±1.54 and DCM extract 61.72±0.59 in 1 mg/mL extract concentration of mushroom. As a result, edible C. cibarius mushroom could be a natural antioxidant source.

The article is devoted to assessing the level of pollution of edible mushrooms by lead, cadmium, zinc and copper in the conditions of the Right Bank Forest-Steppe of Ukraine. Studies were carried out in the forest areas of the Vinnitsa and Kalinovsky districts of the Vinnitsa region. The study of the concentration of heavy metals was carried out in the scientific-measuring agrochemical laboratory of the department of ecology and environmental protection of the agronomical faculty on the basis of Vinnitsa National Agrarian University. The concentration of heavy metals in the fruit bodies of the fungi studied was determined by atomic absorption spectrometry after dry mineralization. Analysis of the anthropogenic impact on the environment shows that the intensity of contamination by heavy metals of all components of the environment in some areas is growing rapidly, to a certain extent leads to contamination of natural food raw materials with these toxins. According to the study revealed an excess of the maximum permissible concentrations of cadmium in Gyroporus cyanescens (Lat.) by 1.6 times, Laetiporus sulphureus (Lat.) — by 1.5 times, Boletus regius (Lat.) — by 1.4 times, Xerocomus (Lat.) — by 1.7 times, Russula (Lat.) — by 6.5 times, Boletus edulis (Lat.) — by 1.7 times, Amanita rubescens (Lat.) — by 1.5 times, Leccinum scabrum (Lat.) — by 1.7 times, Leccinum aurantiacum (Lat.) — by 1.3 times and Armillaria mellea (Lat.) — by 1.7 times, while the concentration of lead, zinc and copper was lower than MPC. At the same time, it should be noted that the highest concentration of lead was found in Armillaria mellea (Lat.) compared to Cantharellus cibarius (Lat.), Gyroporus cyanescens (Lat.), Laetiporus sulphureus (Lat.), Boletus regius (Lat.), Xerocomus (Lat.), Russula (Lat.), Boletus edulis (Lat.), Amanita rubescens (Lat.), Leccinum scabrum (Lat.) and Leccinum aurantiacum (Lat.) by 1.4, 1.3, 1.07, 1.2, 1.03, 1.4, 1.2, 1.07, 1.1 and 1.3 times, respectively. The concentration of cadmium was the highest in Russula (Lat.), in comparison with Cantharellus cibarius (Lat.), Gyroporus cyanescens (Lat.), Laetiporus sulphureus (Lat.), Boletus regius (Lat.), Xerocomus (Lat.), Boletus edulis (Lat.), Amanita rubescens (Lat.), Leccinum scabrum (Lat.), Leccinum aurantiacum (Lat.) and Armillaria mellea (Lat.) by 36, 1.4, 14.4, 15.4, 12.7, 12.7, 14.4, 12.7, 16.6 and 12.7 times, respectively. The concentration of zinc was the highest in Boletus edulis (Lat.), in comparison with Cantharellus cibarius (Lat.), Gyroporus cyanescens (Lat.), Laetiporus sulphureus (Lat.), Boletus regius (Lat.), Xerocomus (Lat.), Russula (Lat.), Amanita rubescens (Lat.), Leccinum scabrum (Lat.), Leccinum aurantiacum (Lat.) and Armillaria mellea (Lat.) by 1.7, 1.6, 2.3, 1.03, 1.5, 1.02, 1.7, 2.7, 1.1 and 154 times, respectively. The concentration of copper was the highest in Armillaria mellea (Lat.), in comparison with Cantharellus cibarius (Lat.), Gyroporus cyanescens (Lat.), Laetiporus sulphureus (Lat.), Boletus regius (Lat.), Xerocomus (Lat.), Russula (Lat.), Boletus edulis (Lat.), Amanita rubescens (Lat.), Leccinum scabrum (Lat.) and Leccinum aurantiacum (Lat.) by 8.7, 4.4, 46.6, 15.5, 11.2, 4.4, 10.8, 17.5, 4 and 20 times, respectively.

Background The Guatemalan Highlands is a region of great but so far poorly known mycological diversity. People living in this area have long used wild fungi as a source of food and income. However, our knowledge of the ethnomycological practices of the Mayan peoples of Guatemala is still rudimental, especially if compared with information reported for the neighboring region of Mexico. Among the main indigenous groups of the Maya people inhabiting the highlands of Central Guatemala, stand the Kaqchikel, accounting for nearly 8% of the entire Guatemalan population. The main aim of this study was to record the traditional knowledge and use of edible wild mushrooms by inhabitants of the municipality of San Juan Sacatepéquez that lies at the heart of the Kaqchikel area in the central highlands of Guatemala, also describing the relevant selling practices and dynamics. A secondary aim was to compare the diversity and composition of the mushroom assemblage offered at the market with the macrofungal diversity of woods in the area. Methodology This study is the result of 4 years of ethnomycological research, conducted through continuous visits to the municipal market and focused interviews with collectors and vendors. Field sampling in pine-oak forested areas surrounding San Juan Sacatepéquez, from where the mushrooms sold at the market are foraged, were also conducted, in the presence of local collectors. Results The results show a significant richness of species sold in the market, a network of commerce of purchase, sale, and resale of several species, with relatively stable prices, and knowledge about edible and inedible species that is transmitted mainly within the family nucleus. The business of selling mushrooms in the market is an exclusive activity of women, who are supplied by collectors or by other vendors. Fungi are sold and bought only as food, while no consumption of hallucinogenic mushrooms or medicinal mushrooms was recorded. Several species of Amanita, Cantharellus, Boletus, Lactarius, and Russula were those most commercialized in the 4 years of the study, but we also spotted fungi never reported before as consumed in the country, including Gastropila aff. fumosa (= Calvatia fumosa) and several species of Cortinarius. Field sampling in nearby pine-oak forests confirmed an elevated local macrofungal diversity. Conclusion Our study unveiled the contemporary wealth of Kaqchikel culture for what concerns mushrooms, demonstrating that mushrooms continue to be culturally and economically important for these communities despite the erosion of traditional knowledge. Our results also confirmed the need to investigate in greater detail the Guatemalan mycodiversity that is vast and poorly known. Electronic supplementary material The online version of this article (10.1186/s13002-019-0310-7) contains supplementary material, which is available to authorized users.

Purpose Mushrooms have always been treasured and appreciated, thanks to their nutritional as well as medicinal properties. The purpose of this paper is to reveal these properties such as antioxidant activities and enzyme inhibition potentials of three mushroom species from Ordu, Turkey. Furthermore, phenolic profiles which are believed to be source of these properties are investigated. Design/methodology/approach The assays followed to determine total phenolic, flavonoid and antioxidant contents, antioxidative and enzyme inhibition activities are spectrophotometric methods. Phenolic profiles of mushroom samples were investigated by using the HPLC system. Findings The highest phenolic content was found for Lactarius deliciosus. Pyrogallol was the major phenolics in all three samples. Total phenolic content values supported that finding. But, total flavonoid content was the maximum for Cantharellus cibarius. The magnitude of the antioxidant activities revealed by the different tests varied between species. Furthermore, inhibition potential degrees on acetylcholinesterase, butrylcholinesterase, tyrosinase and urease activities were worth mentioning. Lactarius pyrogalus had anti-tyrosinase activity with a 26.229 percent inhibition rate. IC50 values for urease inhibition effect of mushroom samples were in the range of 0.37–0.90 mg/mL. Originality/value Enzyme inhibitory potentials for L. pyrogalus was revealed first time. On the other hand, studies on anti-tyrosinase activity of mushrooms are very rare, while almost no studies have been conducted on anti-urease activity. So, the results of present study are very valuable. Thus, the obtained data not only increase the ecological importance of the region but also provide significant contributions to the literature in terms of new sources rich in antioxidants.

The aim of the study was to determine 16 elements by FAAS and ICP-AES in ca. 1500 samples of 22 species of mushrooms collected from different regions of Poland and the area around Umea in Sweden. Chemometric techniques were applied to differentiate samples with respect to their geographical origin and interspecies differentiation. Samples of Cantharellus cibarius (Fr.), Boletus edulis (Bull.) and Leccinum scabrum (Bull.) Gray from Morąg, Augustow, the Zaborski Landscape Park, Tarnobrzeg and Umea were discriminated by factor 1 and factor 2. Some species, i.e. Cantharellus cibarius, Boletus edulis, Boletus pinophilus (Pilat & Dermek), Leccinum aurantiacum (Bull.) Gray, Leccinum scabrum and Leccinum versipelle (Fr. & Hok) Snell from one region of Poland (Augustow or Morąg) were discriminated by K, Na, Mg, Ca, Fe, Zn, Cu, Mn and Cd. The results enabled an assessment of the hypothetical percentage realisation of the recommended dietary intake (RDA) for the bio-elements in question and of provisional tolerable weekly intakes (PTWI) of toxic metals from the consumption of 100 g of mushrooms. The most abundant element in all the mushroom samples was K, especially in Gomphidius glutinosus (Schaeff. ex Fr.) (Umea - Sweden) and Cantharellus cibarius (Poland - Morąg). Lycoperdon perlatum (Pers.) from Poland and Sweden tended to accumulate the highest levels of Mg, Fe, Zn and Cu. The highest percentage of RDA was obtained for K, Mg and Cu. Based on the estimated PTWI, it can be concluded that no health hazard is associated with the consumption of these mushrooms.

This article is the tenth series of the Fungal Diversity Notes, where 114 taxa distributed in three phyla, ten classes, 30 orders and 53 families are described and illustrated. Taxa described in the present study include one new family (viz. Pseudoberkleasmiaceae in Dothideomycetes), five new genera (Caatingomyces, Cryptoschizotrema, Neoacladium, Paramassaria and Trochilispora) and 71 new species, (viz. Acrogenospora thailandica, Amniculicola aquatica, A. guttulata, Angustimassarina sylvatica, Blackwellomyces lateris, Boubovia gelatinosa, Buellia viridula, Caatingomyces brasiliensis, Calophoma humuli, Camarosporidiella mori, Canalisporium dehongense, Cantharellus brunneopallidus, C. griseotinctus, Castanediella meliponae, Coprinopsis psammophila, Cordyceps succavus, Cortinarius minusculus, C. subscotoides, Diaporthe italiana, D. rumicicola, Diatrypella delonicis, Dictyocheirospora aquadulcis, D. taiwanense, Digitodesmium chiangmaiense, Distoseptispora dehongensis, D. palmarum, Dothiorella styphnolobii, Ellisembia aurea, Falciformispora aquatic, Fomitiporia carpinea, F. lagerstroemiae, Grammothele aurantiaca, G. micropora, Hermatomyces bauhiniae, Jahnula queenslandica, Kamalomyces mangrovei, Lecidella yunnanensis, Micarea squamulosa, Muriphaeosphaeria angustifoliae, Neoacladium indicum, Neodidymelliopsis sambuci, Neosetophoma miscanthi, N. salicis, Nodulosphaeria aquilegiae, N. thalictri, Paramassaria samaneae, Penicillium circulare, P. geumsanense, P. mali-pumilae, P. psychrotrophicum, P. wandoense, Phaeoisaria siamensis, Phaeopoacea asparagicola, Phaeosphaeria penniseti, Plectocarpon galapagoense, Porina sorediata, Pseudoberkleasmium chiangmaiense, Pyrenochaetopsis sinensis, Rhizophydium koreanum, Russula prasina, Sporoschisma chiangraiense, Stigmatomyces chamaemyiae, S. cocksii, S. papei, S. tschirnhausii, S. vikhrevii, Thysanorea uniseptata, Torula breviconidiophora, T. polyseptata, Trochilispora schefflerae and Vaginatispora palmae). Further, twelve new combinations (viz. Cryptoschizotrema cryptotrema, Prolixandromyces australi, P. elongatus, P. falcatus, P. longispinae, P. microveliae, P. neoalardi, P. polhemorum, P. protuberans, P. pseudoveliae, P. tenuistipitis and P. umbonatus), an epitype is chosen for Cantharellus goossensiae, a reference specimen for Acrogenospora sphaerocephala and new synonym Prolixandromyces are designated. Twenty-four new records on new hosts and new geographical distributions are also reported (i.e. Acrostalagmus annulatus, Cantharellus goossensiae, Coprinopsis villosa, Dothiorella plurivora, Dothiorella rhamni, Dothiorella symphoricarposicola, Dictyocheirospora rotunda, Fasciatispora arengae, Grammothele brasiliensis, Lasiodiplodia iraniensis, Lembosia xyliae, Morenoina palmicola, Murispora cicognanii, Neodidymelliopsis farokhinejadii, Neolinocarpon rachidis, Nothophoma quercina, Peroneutypa scoparia, Pestalotiopsis aggestorum, Pilidium concavum, Plagiostoma salicellum, Protofenestella ulmi, Sarocladium kiliense, Tetraploa nagasakiensis and Vaginatispora armatispora).

Many mushroom species from the Kichevo valley (Republic of Macedonia) are consumed by the native human population regardless of the heavy metal content and uptake in the human body. The aim of this study was to evaluate the impact of the thermal power plant Oslomej on the extent of heavy metal accumulation in the fruiting bodies of selected fungi. The material of fruiting bodies of 15 species of wild fungi and soil samples were collected from 11 localities at three distances (0.5–0.75, 2.5–3.5, and 6–8 km, respectively) from the thermal power plant Oslomej in the period April 2012 to May 2014. We analyzed the content of seven heavy metals (Ni, Cu, Zn, Fe, Mn, Cd, and Pb) by atomic absorption spectroscopy in the fruiting bodies that were not rinsed before drying. Significant correlation between the distance and content of certain heavy metals was found in 10 fungal species, especially in the cases of Ni and Cu and less in the cases of Cd, Pb, and Fe. Such correlations between heavy metal content in soils (total and extractable) and fruiting bodies were found mostly in the cases of Mn, Zn, Fe, Cd, and Cu. Also, the canonical discriminant analysis showed the impact of thermal power plant Oslomej on heavy metal patterns in Boletus aestivalis, Russula cyanoxantha, Cantharellus cibarius, and Hypholoma fasciculare. It can be concluded that both the pollution from the thermal power plant Oslomej and soil composition determine heavy metal content in the fruiting bodies of wild fungi.

Species of fleshy yellow Cantharellus are known as chanterelles, which are among the most popular wild edible mycorrhizal mushrooms in the world. However, pure culture isolates of Cantharellus are rare. We report an efficient isolation technique of the Japanese golden chanterelle, Cantharellus anzutake, from its ectomycorrhizal root tips. Field-sampled fresh ectomycorrhizal root tips of C. anzutake on various hosts such as pines, spruce, and oaks were vortexed with 0.005% Tween 80 solution, surface sterilized with 1% calcium hypochlorite solution, rinsed with sterilized distilled water, and placed on modified Norkrans’ C (MNC) agar plate medium. Most ectomycorrhizal root tips of C. anzutake produced yellowish mycelial colonies within a few months. In contrast, tissue isolation from basidiomata provided limited cultures of C. anzutake but much contamination of bacteria and molds, even on media that contained antibiotics. The established C. anzutake cultures had clamp connections on the hyphae and contained intracellular oily droplets. These cultured isolates were identified as C. anzutake by sequence analysis of the rRNA internal transcribed spacer (ITS) region and translation elongation factor EF1-alpha (tef-1) genes.

Fascioliasis is a disease of livestock which is now recognized as an emerging disease in humans. Cantharellus cibarius and Ganoderma applanatum are known for their medicinal properties. The use of ethanolic extracts of these macrofungi against the eggs and miracidia of Fasciola spp. is a promising method to break the parasite transmission cycle. The aim of the study is to evaluate the inhibitory effects of ethanolic extracts of the mushrooms on eggs and miracidia of Fasciola spp. Concentrated eggs and miracidia of Fasciola spp. were exposed to different concentrations (1-8 mg/ml) of extracts of Ganoderma applanatum (GEE) and Cantharellus cibarius (CEE) at different time intervals. GEE showed superior antiparasitic activities when compared to CEE at all concentrations tested. Significant positive correlations were observed between the concentration of GEE and mortality in miracidia (r=0.980, P P G. applanatum and C. cibarius have ovicidal and miracicidal activities. While G. applanatum showed excellent activities, activities in C. cibarius were moderate. Therefore, these mushroom extracts can be regarded as promising sources of bioactive compounds that could be developed into ovicides and miracicides.

Antibiotic resistance has become a global concern and threatens the clinical efficacy of many drugs, leading to increased screening of several sources of potential antimicrobial substances. Mushrooms have long been recognized as a valuable source of nutritive and pharmacologically active compounds. Our previous studies showed that the acetone, ethanol, methanol, and cold water crude extracts of various mushrooms-Amanita and Cantharellus species, Ganoderma lucidum, and Lactarius kabansus-exhibited high antibacterial activity against Salmonella typhi. The objective of this study was to isolate, characterize, and identify antibacterial compounds from these crude mushroom extracts. The crude extracts were separated by preparative thin-layer chromatography. Fractions (n = 99) were obtained and screened for antimicrobial activity against S. typhi by using the MTT assay. Of the isolated components, 13 exhibited high inhibitory activity against the growth of S. typhi, with half-maximal inhibitory concentrations ranging from 206 to 619 µg/mL. Some of the highly potent antibacterial compounds were identified by using liquid chromatography-mass spectrometry. Terpenoids (lucidenic acid M and cavipetin D), a phospholipid (C16 sphinganine), and fatty acid amines (stearamide and palmitic amide) were some of the compounds found to be responsible for the antibacterial activity observed. The importance of local mushrooms as sources of antibacterial compounds was revealed. The high inhibitory activity of some mushroom extracts strongly suggests that the mushrooms contain compounds that have great potential for use in developing therapeutic agents against infections caused by S. typhi.

The purpose of this paper was discovering the diversity of macromycetes in the territory of Batocina (Serbia). Field studies, which lasted more than a year, revealed the presence of 200 species of macromycetes. The identified species belong to phyla Basidiomycota (191 species) and Ascomycota (9 species). The biggest number of registered species (100 species) was from the order Agaricales. Among the identified species was one strictly protected - Phallus hadriani and seven protected species: Amanita caesarea, Marasmius oreades, Cantharellus cibarius, Craterellus cornucopiaodes, Tuber aestivum, Russula cyanoxantha and R. virescens; also, several rare and endangered species of Serbia. This paper is a contribution to the knowledge of the diversity of macromycetes not only in the territory of Batocina, but in Serbia, in general.

A total of 82 species and 2 variations of medicinal and edible macrofungi (Basidiomycota and Ascomy-cota) have been collected and identified in Mazandaran Province of Northern Iran. Among these species, 58 possess medicinal and culinary properties, whereas 21 possess only medicinal properties (antiinflammatory, antimicrobial, an-timutagenic, antioxidant, antitumor, antiplatelet, hypoglycemic, hypocholesterolemic, neurotropic, hypotensive, insec-ticidal, immunomodulating, mitogenic/regenerative, spasmolytic, etc.). No medicinal effect has yet been reported for 4 species (Cantharellus infundibuliformis, Mycena inclinata, Suillus collinitus, Xerocomus porosporus) and 2 variations (Pluteus cervinus var. albus, Russula cyanoxantha var. variata) of edible species. Among the listed species, 15 (such as Auricularia auricula-judae, Hericium erinaceus, Fomes fomentarius, Flammulina velutipes, Ganoderma lucidum, Lyophyllum decastes, Pleurotus ostreatus, Tremella mesenterica) are currently used for manufacturing organic cosmetic products. The well-known mushrooms with medicinal properties (e.g., Auricularia auricula-judae, Flammulina velutipes, Hericium erinaceus, Ganoderma lucidum, G. tsugae, G. adspersum, Trametes versicolor) and excellent edibility (e.g., Boletus edulis, Cantharellus cibarius, Morchella esculenta, Pleurotus ostreatus) are widely distributed in the studied area. The biological resources of macrofungi growing in Mazandaran Province of Northern Iran possess medicinal, nutritional, and cosmetic values and could be further used for biotechnological exploitation to develop mushroom-derived pharmaceuticals, nutriceuticals, nutraceuticals, and cosmeceuticals.

Cantharellus cibarius Fr. (Figs 5-6) In Systema Mycologicum (Lundae) 1: 318 (1821). Agaricus chantarellus L., Species Plantarum 2: 1171 (1753). — Merulius chantarellus (L.) Scop., Flora Carniolica, ed. 2, 2: 461 (1772) ‘‘ cantharellus’’. — Cantharellus flavescens Lam., Encyclopedie 1 (2): 694 (1785) [nom. nov. based on Agaricus chantarellus L.]. — Cantharellus edulis Pers., Neues Magazin für die Botanik 1: 106 (1794) [nom. illeg. Art. 52.1]. — Cantharellus vulgaris Gray, Natural Arrangement of British Plants 1: 636 (1821) [nom. illeg. Art. 52.1]. — Craterellus cibarius (Fr.: Fr.) Quél., Flore mycologique de la France et des pays limitrophes: 37 (1888). — Alectorolophoides cibarius (Fr.: Fr.) Earle, Bulletin of the New York Botanical Garden 5: 407 (1909). — Cantharellus cibarius f. pallidus R. Schulz in Michaël, Führer für Pilzfreunde 1: no. 82 (1923). — Cantharellus cibarius var. inodorus Velen., Novitates Mycologicae: 36 (1939). — Cantharellus cibarius var. atlanticus Romagn., Docums Mycology 25 (98-100): 421 (1995). — Cantharellus parviluteus Fern. Sas., Pérez-de-Greg. & Eyssart., Bulletin de la Société mycologique de France 119 (3-4): 262 (2003). MYCOBANK. — 201371. NEOTYPE. — United Kingdom, England, Devon, Bovey Tracey, Tradbere Down, on the ground near Betula, 3.X.1992, N. W. Legon, K (M)22132. (Olariaga et al. 2016). MATERIAL EXAMINED. — Tuan County, Jilin Prov., China. Changbai Mountain Natural Reserve, 7.VIII.2010, Wang XH2580, (HKAS [HKAS 58234]). HABIT, HABITAT AND DISTRIBUTION. — Single to caespitose, forming ectomycorrhizae with Pinus koraiensis or Quercus mongolica. Present in Jilin Province, northeastern China. DESCRIPTION OF THE CHINESE COLLECTION Basidiomata Fleshy, medium-sized. Pileus Convex-planar, later becoming more or less planar with age; margin incurved or straight, regular or folded, smooth, thin; with surface smooth to minutely pubescent, orange-yellow (3A7, 3A8) to bright yellow at the margin (1A7, 2A6). Context Solid. Hymenophore With well-defined gill-like folds, up to 3 mm, decurrent, forking and anastomosing only near the cap margin, pale yellow to light yellow (3A2, 3A3). Stipe Solid, subcylindrical tapering downwards, concolorous with the hymenophore with a yellow tint (2A6). Odor Mild, agreeable, sometimes fruity. Taste Mild, agreeable. Spore print No color. Basidiospores Oblong to reniform, smooth, colorless, and hyaline, thinwalled, 8-10 × 5-6 µm. Xm= 9.14 × 5.08 µm, Q = 1.60-2.0. Basidia 80-105 × 7-9 (-10) µm, long and slender, 5-6 sterigmata. Cystidia Absent. Hymenophoral trama Composed of intermingled filamentous hyphae 3.0-5.0 µm diam., colorless, thin-walled in an irregular arrangement. Pileipellis Mainly of the cutis type, in some areas of the surface, the terminal hyphae may be erect, forming an intricate trichoderm and cluster of hyphae. Hyphae of the pileipellis 4.0-6.0 µm diam., intermingled in a compact (cutis) or slightly loose (trichoderm) arrangement, cylindrical, hyaline to yellowish, often with light yellow contents in some, decidedly light yellow-colored, slightly thick-walled (Pileus trama Composed of cylindrical to inflated hyphae, 4-6 µm diam., slightly thick-walled (Clamp connections Present in all tissues examined., Published as part of Shao, Shi-Cheng, Liu, Pei-Gui, Wei, Tie-Zheng & Herrera, Mariana, 2021, New insights into the taxonomy of the genus Cantharellus in China: epityfication of C. yunnanensis W. F. Chiu and the first record of C. cibarius Fr., pp. 25-37 in Cryptogamie, Mycologie 20 (3) on pages 32-34, DOI: 10.5252/cryptogamie-mycologie2021v42a3, http://zenodo.org/record/7815227, {"references":["OLARIAGA I., MORENO G., MANJON J. L., SALCEDO I., HOFSTET- TER V., RODRIGUEZ D. & BUYCK B. 2016. - Cantharellus (Cantharellales, Basidiomycota) revisted in Europe through a multigene phylogeny. Fungal Diversity 83: 263 - 292. https: // doi. org / 10.1007 / s 13225 - 016 - 0376 - 7"]}

Yellow chanterelles are one of the most common edible mushrooms found in Yunnan Province, China. Previous studies identified the species of these mushrooms as Cantharellus cibarius Fr. based on morphology alone. However, the diversity of yellow chanterelles comprises many species within the C. cibarius complex, including C. yunnanensis W.F. Chiu. We hypothesized that the latter species is widely marketed in Yunnan Province and does not actually belong to C. cibarius. Hence, to confirm the species identity, taxonomic re-evaluation using integrated taxonomic approaches is required. Herein, samples collected from different markets and from near the type locality were subjected to morphological and molecular analyses. We generated tef-1α sequences and analyzed them using the maximum likelihood and Bayesian inference methods. The study revealed that the specimens previously identified as C. cibarius with a wide distribution in southwestern China are indeed C. yunnanensis. Our morphological assessment was markedly influenced by the revision of the type specimen (C. yunnanensis) and its original description, as some characteristics of the specimen did not match the description (e.g., the size and shape of the spores). Similarly, our single-locus phylogenetic analysis strongly supported the identification of the marketed species in Southwestern China (Guizhou and Yunnan Provinces) as C. yunnanensis instead of C. cibarius. Following our findings, we epitypified C. yunnanensis. Additionally, we collected, identified, and confirmed the presence of C. cibarius in northeastern China.

Cancer incidence rates are on the increase worldwide. The most common brain cancer in adults is glioblastoma. Currently available treatment modalities are limited and natural products such as mushrooms could enhance them. Apart from nutritional value, mushrooms are an excellent source of bioactive compounds and therefore could be used to treat various disorders. The aim of the study was to assess the anti-glioma potential of selected mushrooms on U87MG, LN-18 glioblastoma and SVGp12 normal human astroglial cell lines. The materials were Cantharellus cibarius, Coprinus comatus, Lycoperdon perlatum and Lactarius delicious. Aqueous, 70 % ethanol or 95 % ethanol extracts from mushrooms were used for analysis including assessment of antioxidant activity by DPPH assay, cell viability by MTT assay, DNA biosynthesis by thymidine incorporation assay, activity of metalloproteinase by gelatin zymography and cell cycle assay by flow cytometry. Mushroom extracts influenced the viability and DNA biosynthesis of cancer cells. Activity of ethanol mushroom extracts was stronger than that of aqueous extracts. Anti-glioma mechanism consisted in inhibition of cancer cell proliferation and induction of apoptosis associated with arrest of cells in subG1 or G2/M phase of cell cycle, and inhibition of metalloproteinases activity. Among investigated mushrooms, L. deliciosus and C. comatus showed the greatest anti-glioma potential.

Although DDT, γ-HCH and PCBs have been almost completely withdrawn from world production and use, they are still present in the environment. Mushrooms are eagerly collected and consumed local raw material in north-eastern part of Poland. The aim of the study was to determine the content of DDT, γ-HCH and PCB residues in popular species (Boletus badius, Boletus edulis, Cantharellus cibarius) and to estimate the human exposure of uptaking of these compounds with mushrooms. The content of γ-HCH, DDT and PCB was determined using gas chromatography. The presence of analyzed compounds was observed in all the species under study. The highest content of γ-HCH and ΣDDT was determined in C. cibarius − 7.19 and 180.37 µg/kg of lipids, respectively, whereas the highest amount of ΣPCB was observed in B. edulis − 20.89 µg/kg of lipids. The contents were low and did not pose a threat to human health.

The authors discuss several African Cantharellus Adans.: Fr. species with lilac-purplish tinges on the pileus or stipe surface collected in the Central African rain forest or West African gallery forests. The morphology of C. longisporus Heinem. is discussed on the basis of new Central African collections and found profoundly different from two previously described subspecies from Madagascar which are here recombined as C. littoralis (Buyck & Randrianjohany) Buyck, comb. et stat. nov., and C. isaloensis (Buyck & Eyssartier) Buyck, comb. et stat. nov. Cantharellus xanthocyaneus Ndolo Ebika & Buyck, sp. nov., a new look-alike of C. cyanoxanthus R.Heim ex Heinem. with considerably smaller basidiospores is described, as well as a new chanterelle species from Togo, viz. C. violaceoflavescens De Kesel, Guelly, L.A.Parra & Buyck, sp. nov., that in the field is reminiscent of C. littoralis (Buyck & Randrianjohany) Buyck, comb. et stat. nov. A multigene phylogeny places both new species Cantharellus xanthocyaneus Ndolo Ebika & Buyck, sp. nov., and Cantharellus violaceoflavescens De Kesel, Guelly, L.A.Parra & Buyck, sp. nov., in the subgenera Parvocantharellus Eyssart. & Buyck and Pseudocantharellus Eyssart. & Buyck respectively, and Cantharellus littoralis (Buyck & Randrianjohany) Buyck, comb. et stat. nov., in the subgenus Cinnabarini Buyck & V.Hofst.

Increasing evidence suggest that bacteria form diverse communities in various eukaryotic hosts, including fungi. However, little is known about their succession and the functional potential at different host development stages. Here we examined the effect of fruiting body parts and developmental stages on the structure and potential function of fungus-associated bacterial communities. Using high-throughput sequencing, we characterized bacterial communities and their associated potential functions in fruiting bodies from ten genera belonging to four major mushroom-forming orders and three different developmental stages of a model host species Cantharellus cibarius. Our results demonstrate that bacterial community structure differs between internal and external parts of the fruiting body but not between inner tissues. The structure of the bacterial communities showed significant variation across fruiting body developmental stages. We provide evidence that certain functional groups, such as those related to nitrogen fixation, persist in fruiting bodies during the maturation, but are replaced by putative parasites/pathogens afterwards. These data suggest that bacterial communities inhabiting fungal fruiting bodies may play important roles in their growth and development.

The golden chanterelle represents one of the commonly found, edible mushrooms that is highly valued in various cuisines. The present study focused on assessing the requirements of Cantharellus cibarius such as pH, temperature, as well as the carbon and nitrogen sources for mycelial growth. Optimization of the growth parameters was carried out by one-factor-at-a-time method. The optimal pH and temperature were determined to be 6.0 and 22.5 °C, respectively. Among the various carbon sources studied, sucrose at a concentration of 2% gave maximum mycelial growth and proved to be the most suitable one. Amongst the nitrogen sources studied, peptone, ammonium sulphate, and sodium nitrate, gave the maximum mycelial growth at an optimized concentration of 0.5%. In the presence of beef extract and yeast extract, a change in colony pigmentation from yellow to dark grey was observed. Finally, the carbon to nitrogen ratio of 2:0.5 proved to be optimal for mycelial growth. This study is the first report on the optimisation of in vitro growth requirements of C. cibarius.

The concept of cultural significance and its quantitative evaluation are useful to recognize which species (both edible and toxic) are the most relevant within a specific community. This work lists the most culturally significant edible and toxic species for the Tseltal groups in the Highlands of Chiapas. It also evaluates whether the composition and significance of these is different between the different Tseltal communities, proving the hypothesis that the edible and toxic species are not the same nor do they have the same level of significance in different settlements. One hundred and ninety-three interviews were carried out with Tseltal participants regarding edible and toxic mushrooms. The information was analyzed both qualitatively and quantitatively, using multivariate methods. People recognized 25 edible taxa whose names differ across the seven communities. The most frequently mentioned and consumed taxa are Amanita complex. caesarea, Cantharellus complex. cibarius and Agaricus spp. The classification analysis based on the relative frequency of mention for edible species, shows a variation pattern explained by the geographic, cultural and linguistic variation between Tseltal groups. For toxic mushrooms, people recognized and assigned names to 17 taxa. Only 17% of the interviewees assign at least one name to these species. The results show that Tseltal peoples from the Highlands of Chiapas are highly mycophilic. Furthermore, clear differences within the seven Tseltal groups are clear, not only with regards to lexical variations, but also regarding the number of known species, the species that are known and their degree of significance.

Release of bioelements and phenolic compounds from edible mushrooms (Agaricus bisporus, Cantharellus cibarius, and Lentinula edodes) enriched with zinc, selenium, l -phenylalanine, alone and as a mixture was examined using a simulated human gastrointestinal digestion method. Due to the extensive amount of data obtained, in order to interpret them more precisely in the work, the methods of chemometric analysis (Cluster Analysis—CA and Principal Compenent Analysis—PCA) were additionally applied. The results showed mycelium of L. edodes has the best health-promoting properties and addition of mixture to the media increased significantly the synthesis of p-hydroxybenzoic and protocatechuic acid (267 and 16.3 mg/100 g d.w.). After extraction into artificial digestive juices, 97.4 mg/100 g d.w. p-hydroxybenzoic acid and 15.6 mg/100 g d.w. of protocatechuic acid were released. The greatest amounts of Se and Zn were extracted from enriched A. bisporus mycelium (32.3 and 342 mg/100 g d.w., respectively). This study confirmed that mycelium might prevent nutritional deficiencies in the diet through use of functional foods.

Serpentine soil contains highly heavy metals, such as manganese, chromium, cobalt, and nickel,which could bean inappropriate growthmediaofmostplants. Someplants thatfound able to grow optimally on South Kalimantan serpentine soil have been known to do association with ectomycorrhizal fungi. This research aimed to obtain and characterize mushrooms assumed as ectomycorrhizal fungi indigenous South Kalimantan serpentine soil. This study used field exploration of fungal fruiting bodies and identified the genus based on morphological characters of fruiting bodies such as shape, size, and ornamentation, which are unique for the genus identification, then compared the characteristics on mushroomexpert.com. The mushrooms were also confirmed of genera assumed as ectomycorrhizal fungi based on mycorrhizas.info. Seven fruiting bodies were obtained and classified as Cantharellus (Ct), Chlorophyllum (Ch1 and Ch2), Lycoperdon (Ly), Ramaria (Rm1 and Rm2), and Thelephora (Tp). The results showed that all of those fruiting bodies belong to Basidiomycetes. There were 4 genera of Cantharellus, Lycoperdon, Ramaria, and Thelephora, assumed as ectomycorrhizal fungi. But Chlorophyllum genus was never reported as ectomycorrhizal fungus

This chapter contains updated information on the diversity of ectomycorrhizal mushrooms, especially those edible that exist in Guatemala, their hosts, and distribution. The country has many different environments and vegetation types that provide habitats for a large diversity of mushrooms, particularly ectomycorrhizal associated with Abies, Alnus, Arbutus, Pinus, and Quercus, depending on the altitude and geographic region where these fungi grow. It also makes reference to species that seem to have an origin in western North America and not only from the Eastern region, what supposes novel information, in accordance with recent fungal findings and with the geological and natural history of the country. New species and complexes of species are cited, especially for some edible mushrooms that belong to the genera Amanita, Boletus, Cantharellus, and Lactarius, as well as updated ethnomycological information that includes mushroom stones and commerce of local edible species.

Agaricus bisporus, Cantharellus cibarius, Imleria badia, and Lentinula edodes are among the most popular species of edible mushrooms in Poland. These edible mushrooms are an important source of biologically active substances exhibiting beneficial (e.g., antioxidant, antitumor, antimicrobial, anti-inflammatory) effects on the human body. The fruiting bodies of edible mushrooms are also a valuable source of lovastatin, which belongs to a group of compounds, called statins, commonly used as cholesterol-lowering drugs. Due to the presence of lovastatin, edible mushrooms can be useful in the prevention of hypercholesterolemia. Therefore, the aim of this study was to determine the content of lovastatin in the selected species of edible mushrooms and to evaluate its release into artificial digestive juices. This study was the first to determine the release of lovastatin into digestive juices after the extraction of materials obtained from edible mushrooms. The largest amount of lovastatin was found in the fruiting bodies of C. cibarius (67.89 mg/100 g d.w.), and the smallest in those of L. edodes (0.95 mg/100 g d.w.). The amount of lovastatin released from the extracts of the examined species into digestive juices was found to be relatively low. The highest content after incubation in artificial gastric juice was detected for the fruiting bodies of L. edodes (0.02 mg/100 g d.w.) and after incubation in the intestinal juice for the mycelium from the in vitro cultures of L. edodes (0.51 mg/100 g d.w.). Thus, the results of the present study showed that due to its ability to accumulate lovastatin from culture medium, L. edodes mycelium can be used to obtain a product with increased hypolipidemic activity.

The taxa designated as macrofungi represent a wealth of health-promising fungi rich in a wide spectrum of mycochemicals of nutraceutical and therapeutic value (Wasser 2011; Sanico et al. 2014; Rahi and Malik 2016; Hoeksma et al. 2019). However, only a small fraction out of a vast diversity of fungi in millions (nearly 3–5.1 millions) has been explored for nutrients and therapeutic components. In the last few decades, massive research has been carried out and continues to grow incessantly to explore the biochemistry of macrofungi (De Silva et al. 2013; Flores Jr et al. 2014; Diaz-Godinez 2015; Badalyan 2016; Kalac 2016; Kivrak et al. 2016; Rathore et al. 2017; Elkhateeb et al. 2019a, b). This is necessary in order to give scientific valorization to the traditional practices of macrofungi especially the medicinal uses. The medicinal species largely belong to the genera Auricularia, Cantharellus, Ganoderma, Pleurotus, Lentinus, Trametes, Tremella, Amanita, etc. and possess a number of high and low molecular weight bioactive constituents responsible for their pharmacological potential (Diksha et al. 2018). Many members of Basidiomycota as well as some of the Ascomycota are rich in antitumor, antimicrobial, antioxidant, hepatoprotective, hypoglycemic, hypolipidemic, immunomodulating as well as prebiotic substances (Lindequist et al. 2010; Chang and Wasser 2012; Giavasis 2013; Mizuno and Nishitani 2013; Wasser 2014; Singh et al. 2017; Chaturvedi et al. 2018; Fernando et al. 2018; Schuffler 2018; Liu et al. 2020). The major bioactive components present in higher fungi are polysaccharides (homo- and hetero-polysaccharides), terpenes (monoterpenoids, sesquiterpenoids, diterpenoids, and triterpenoids), proteins/peptides, glycoproteins, alkaloids, phenolics, tocopherols, ergosterols, and various fatty acids accounting for their biological activities. These are collectively described as “biological response modifiers” because of their multiple biological impacts which trigger the immune response and elevate the curative properties of the human body (Khatua et al. 2013; Barreira et al. 2014; Kumar 2015; Heleno et al. 2015a; Ruthes et al. 2016; Tel-Cayan et al. 2017; Ma et al. 2018; Phan et al. 2018). The crude extracts and isolated bioactive components from sporocarps and/or mycelial biomass of macrofungi have been appraised for their pharmacological activities. These exhibit anticancer, antidiabetic, anti-inflammatory, antimicrobial, antioxidant, immunomodulatory, hepatoprotective, neuroprotective activities through various metabolic pathways (Thu et al. 2020). However, only a few clinical studies have been performed and need much attention for authentication of the medicinal uses of macrofungi to treat human disorders (Wasser 2017).

Almost all world-famous species of edible mycorrhizal mushrooms (EMMs), or closely related species, are growing naturally in China. These include black truffles in the Tuber indicum complex (sister species to T. melanosporum), and T. sinoaestivum (closely related to T. aestivum), Tricholoma matsutake, Boletus edulis, the Amanita hemibapha complex (closely related to A. caesarea), Cantharellus cibarius, Lyophyllum shimeji, Lactarius deliciosus, and Lactarius hatsudake (closely related to L. sanguifluus). In addition, China has her own unique EMM species. Tuber panzhihuanense, for example, a good edible white truffle belonging to the T. borchii group, has been recently described and commercialized in China. Thelephora ganbajun is one of the most likeable and expensive EMMs in Yunnan. Scleroderma yunnanense, possibly the world’s only edible species in this genus, is extensively harvested for food and trading in Yunnan. In China, EMMs are delicacies and important sources of livelihood for farmers. This chapter gives a brief introduction to Chinese EMMs.